Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Comparative Genomic Hybridization: A method for comparing two sets of chromosomal DNA by analyzing differences in the copy number and location of specific sequences. It is used to look for large sequence changes such as deletions, duplications, amplifications, or translocations.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.DNA, Ribosomal Spacer: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).Gene Dosage: The number of copies of a given gene present in the cell of an organism. An increase in gene dosage (by GENE DUPLICATION for example) can result in higher levels of gene product formation. GENE DOSAGE COMPENSATION mechanisms result in adjustments to the level GENE EXPRESSION when there are changes or differences in gene dosage.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Oligonucleotides, Antisense: Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Sequence Analysis: A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.RNA, Complementary: Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed)RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Microarray Analysis: The simultaneous analysis, on a microchip, of multiple samples or targets arranged in an array format.Fixatives: Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Genetic Variation: Genotypic differences observed among individuals in a population.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.DNA Copy Number Variations: Stretches of genomic DNA that exist in different multiples between individuals. Many copy number variations have been associated with susceptibility or resistance to disease.Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.Software: Sequential operating programs and data which instruct the functioning of a digital computer.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Nucleic Acid Amplification Techniques: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.Expressed Sequence Tags: Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Models, Statistical: Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.Data Interpretation, Statistical: Application of statistical procedures to analyze specific observed or assumed facts from a particular study.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.RNA, Neoplasm: RNA present in neoplastic tissue.Genes, Neoplasm: Genes whose abnormal expression, or MUTATION are associated with the development, growth, or progression of NEOPLASMS.Chromosome Aberrations: Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.Genes, Bacterial: The functional hereditary units of BACTERIA.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Cell Line: Established cell cultures that have the potential to propagate indefinitely.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Oligoribonucleotides: A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Quality Control: A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Genome, Plant: The genetic complement of a plant (PLANTS) as represented in its DNA.Gene Duplication: Processes occurring in various organisms by which new genes are copied. Gene duplication may result in a MULTIGENE FAMILY; supergenes or PSEUDOGENES.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Cell Line, Tumor: A cell line derived from cultured tumor cells.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Bacterial Proteins: Proteins found in any species of bacterium.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.DNA, Neoplasm: DNA present in neoplastic tissue.Phosphorothioate Oligonucleotides: Modified oligonucleotides in which one of the oxygens of the phosphate group is replaced with a sulfur atom.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Oligodeoxyribonucleotides, Antisense: Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Protein Array Analysis: Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Thionucleotides: Nucleotides in which the base moiety is substituted with one or more sulfur atoms.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Oligoribonucleotides, Antisense: Short fragments of RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Mice, Inbred C57BLMolecular Weight: The sum of the weight of all the atoms in a molecule.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).Genes, Viral: The functional hereditary units of VIRUSES.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Sequence Homology: The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species.Kinetics: The rate dynamics in chemical or physical systems.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Sequence Analysis, RNA: A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Molecular Probe Techniques: The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.Nucleic Acid Denaturation: Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.RNA, Ribosomal: The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Consensus Sequence: A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.

*  Increased dietary intake of vitamin A promotes aortic valve calcification in vivo.
Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Osteogenesis /...
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*  Role of the POZ zinc finger transcription factor FBI-1 in human and murine adipogenesis.
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*  Hereditary evaluation of multiple developmental abnormalities in the Havanese dog breed.
Novel statistical analysis methods were used to identify four ... -specific microarray was used to identify changes in gene ... Gene Expression Oligonucleotide Array Sequence Analysis Phenotype. From...
http://biomedsearch.com/nih/Hereditary-evaluation-multiple-developmental-abnormalities/17621585.html
*  URMC Research Network -
Molecular Sequence Data. Models, Genetic. Models, ... Models, Biological. Oligonucleotide Array Sequence Analysis. Protein Array Analysis. Disorders Thalassemia. Genetic...
https://urmc.rochester.edu/profiles/display/138107/network/researchareas/categories
*  Browsing BioMedCentral SWORD Deposits by Subject
physiology oligonucleotide array sequence analysis protein...
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*  Estradiol induces cytochrome P450 2B6 expression at high concentrations: implication in estrogen-med
/ metabolism Oligonucleotide Array Sequence Analysis Oxidoreductases...
http://biomedsearch.com/nih/Estradiol-induces-cytochrome-P450-2B6/22484313.html
*  ORBi: Arijs I. - Mucosal gene signatures to predict response to infliximab in patients with ulcerati
; Middle Aged ; Oligonucleotide Array Sequence Analysis/methods ;...
http://orbi.ulg.ac.be/handle/2268/38707
*  Pseudomonas aeruginosa twitching motility-mediated chemotaxis towards phospholipids and fatty acids:
lengths. Analysis of mutants that are deficient in ... Molecular Structure Oligonucleotide Array Sequence Analysis Phospholipids...
http://biomedsearch.com/nih/Pseudomonas-aeruginosa-twitching-motility-mediated/18390654.html
*  GeneChip® Human Genome U133 Plus 2.0 Array | AffymetrixCatalogNav
GeneChip Human Genome U133 Plus 2.0 Array. Affymetrix. Affymetrix Microarray Brands. USB Molecular Biology Reagents. eBioscience Immunology Reagents. Community. Affymetrix Microarray Solutions. GeneChip-compatible Software Providers. About Affymetrix. Contact Us. Products Microarray Solutions Microarray Solutions View all DNA Analysis Solutions Genome-Wide Genotyping for Human Disease Research. RNA Analysis Solutions 3' IVT Expression Analysis. miRNA Analysis. Services, Support, and Training MyGeneChip Custom Array Services. Molecular Pathology Molecular Pathology View all Services and Support Assay Services / Assay Development. eBioscience Immunology Reagents eBioscience Immunology Reagents View all Clinical Antibodies. PCR PCR View all End Point PCR DNA Polymerases. PCR Purification. PCR Clean Up View all products >. qPCR/qRT-PCR DNA Polymerases. RT-PCR DNA Polymerases. PCR Purification. Molecular Biology Enzymes Molecular Biology Enzymes View all Binding Proteins. DNA Polymerases. Molecular Biology Kit...
http://affymetrix.com/estore/browse/products.jsp?productId=131455&categoryId=35760
*  Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets i
... n TOR pathway. Broad Institute of MIT and Harvard. Partnerships Philanthropy Careers Contact Us. What is Broad. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. Home. News & Publications:Scientific Publications Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway. Recent Broad Publications A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Read More / View Supplemental Materials An integrated map of structural variation in 2,504 human genomes. Read More / View Supplemental Materials Somatic mutation in single human neurons tracks developmental and transcriptional history. Rea...
http://broadinstitute.org/publications/broad3659
*  Human OneArray® Gene Expression Microarrays | Phalanx Biotech
Human OneArray Gene Expression Microarrays. Phalanx Biotech. Home Products Genome Microarrays. Human Microarray. Mouse Microarray. Rat Microarray. Rice Microarray. Yeast Microarray. microRNA Microarrays. Human Microarray. Mouse Rat Microarray. Plant Microarray. Hybridization Chambers Diagnostic Products. Chromosomal Microarray. HCC Diagnostic. Documentation Download. Microarray Protocol. Information Sheet. Technical Note - MAQC Performance. Technical Note - Probe Design. REQUEST A QUOTE. Published literature 361. Human Gene Expression Microarrays. Human OneArray v6 Advantages. Highly competitive pricing Outstanding technical support Over 30,000 human genome probes Strong correlation with real-time PCR Excellent sensitivity and dynamic range Compatible with common microarray scanners Competes head-to-head with other microarray platforms Purchase now at our online store. Recent Human OneArray Publications. Heng-hsiung Wu, Wendy W. Hwang-verslues, Wen-hsin Lee, Chun-kai Huang, Pei-chi Wei, Chia-lin Chen, Jin-yuh...
http://phalanxbiotech.com/products/HOA.php
*  DNA microarray experiment
... This is an example of a 'DNA microarray experiment', detailing a particular case to better explain DNA microarray experiments, while enumerating possible alternatives. The nucleic acid of interest is purified: this can be all RNA for expression profiling, DNA for comparative hybridization, or DNA/RNA bound to a particular protein which is immunoprecipitated ChIP-on-chip for epigenetic or regulation studies. In this example total RNA is isolated total as it is nuclear and cytoplasm ic by Guanidinium thiocyanate-phenol-chloroform extraction e.g. The RNA is reverse transcribed with either polyT primers which amplify only mRNA or random primers which amplify all RNA, most of which is rRNA ; miRNA microarrays ligate an oligonucleotide to the purified small RNA isolated with a fractionator, which is then reverse transcribed and amplified. For two-channel arrays, the coupling stage occurs before hybridization, using aminoallyl uridine triphosphate aminoallyl-UTP, or aaUTP and NHS amino-reactive dyes such as cya...
https://en.wikipedia.org/wiki/DNA_microarray_experiment
*  Exploration of global gene expression in human liver steatosis by high-density oligonucleotide micro
For full functionality of ResearchGate it is necessary to enable JavaScript. Here are the instructions how to enable JavaScript in your web browser . Article Exploration of global gene expression in human liver steatosis by high-density oligonucleotide microarray Franck Chiappini Franck Chiappini Remove suggestion Alain Barrier Alain Barrier Remove suggestion Raphaël Saffroy Raphaël Saffroy Remove suggestion Marie-Charlotte Domart Marie-Charlotte Domart Francis Crick Institute Message author Remove suggestion Nicolas Daguès Nicolas Daguès SOLVAY, Lyon, France Message author Remove suggestion Daniel Azoulay Daniel Azoulay Assistance Publique – Hôpitaux de Paris Message author Remove suggestion [more] Inserm 602, Service de Biochimie et Biologie Moléculaire, Hôpital Universitaire Paul Brousse, Université Paris XI, Villejuif Cedex, France. Laboratory Investigation (Impact Factor: 3.68). 03/2006; 86(2):154-65. DOI: 10.1038/labinvest.3700374 Source: PubMed ABSTRACT Understanding the molecular mechanisms underlying...
http://researchgate.net/publication/7424932_Exploration_of_global_gene_expression_in_human_liver_steatosis_by_high-density_oligonucleotide_microarray
*  BaseClear Joins Roche NimbleGen’s Certified Service Provider Program | GEN News Highlights | GE
BaseClear Joins Roche NimbleGen’s Certified Service Provider Program. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Market & Tech Analysis. Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints The Use of High-Content Screening as... GEN News Highlights More » Apr 26, 2011 BaseClear Joins Roche NimbleGen’s Certified Service Provider Program Page 1 of 1 BaseClear has become the first company in Europe and the second provider worldwide to win certification under Roche NimbleGen s certified service provider program for use of the NimbleGen SeqCap EZ Library solution phase workflow for next-generation sequencing. Roche NimbleGen's first CSP in the U.S., Ambry Gene...
http://genengnews.com/gen-news-highlights/baseclear-joins-roche-nimblegen-s-certified-service-provider-program/81245063/?kwrd=BaseClear
*  genetics - What information do microarray image convey? - Biology Stack Exchange
genetics - What information do microarray image convey. - Biology Stack Exchange. Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. What information do microarray image convey. OK I have read that a cell generates 4 kinds of digital to be precise discrete data namely DNA, RNA, Protein sequences that can be encoded as string sequences of nucleotides/ amino acids and micro array image. I would like to know what information do DNA micro array image convey. Sample micro array image:. genetics dna rna gene-expression microarray share. What is the relation of micro array image with gene expression. en.m.wikipedia.org/wiki/DNA_microarray In the context of an expression level experiment, each spot represents a unique oligonucleotide probe. The relative colour intensity of each spot tel...
http://biology.stackexchange.com/questions/17360/what-information-do-microarray-image-convey
*  .. Ambry Genetics to Provide Entire Roche NimbleGen Array Portfolio ..
send to printer gen news highlights apr ambry genetics to provide entire roche nimblegen array portfolio certification adds cgh chip chip dna methylation and gene expression products to ambry s coverage ambry genetics has officially become a certified service provider csp for nimblegen comparative genomic hybridization cgh chromatin immunoprecipiation on chip chip chip dna methylation and gene expression the firm roche nimblegen s first csp in the u s is now certified on all roche nimblegen applications the first csp to attain this status a cap accredited clia certified commercial clinical laboratory ambry genetics will process customer samples for copy number variation dna methylation analysis gene expression analysis and dna protein interactions and chromatin structure the company will use the complete nimblegen dual and single color microarray workflow which includes the high resolution ms microarray scanner ambry was initiated as a roche nimblegen csp in the u s during september the company became a suppl...
http://genengnews.com/keywordsandtools/print/4/22807/
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 4
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Porcine V2 Gene Expression Microarray, 4x44 Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rice Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Salmon Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Tobacco Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Mo...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-4/?viewAll=1&pageSize=25&u=B4747604-32F8-4DE0-9DD1-C94C162B30BF
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 3
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Porcine V2 Gene Expression Microarray, 4x44 Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rabbit Gene Expression Microarray, 4x44K Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rice Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Salmon Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Mod...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-3/?viewAll=1&pageSize=25&sortBy=r&u=5F1CA907-40A1-4813-823E-975369A60CFF
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 4
Read more Articles Videos. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Rabbit Gene Expression Microarray, 4x44K Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rice Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Tobacco Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Tomato Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides resear...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-4/?viewAll=1&pageSize=25&u=BEC53F06-84DA-4036-8F88-B5D217053C55
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 3
Read more Articles Videos. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Porcine V2 Gene Expression Microarray, 4x44 Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rabbit Gene Expression Microarray, 4x44K Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rice Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Sheep Gene Expression Microarray, 8x15k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides resea...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-3/?viewAll=1&pageSize=25&sortBy=r&u=4DC0E1C7-8E7E-4844-AE95-A31AABE1317C
*  Different microarray systems more alike than previously thought ( A multicenter comparison of equipm
Different microarray systems more alike than previously thought A multicenter comparison of equipment t... Home. Biology. Medicine. Technology. Products. News. Definition. Dictionary. Movies. Links. Tags. Search. RSS Navigation Links Biology News Medicine News Biology Products Medicine Products Biology Definition Medicine Definition Biology Technology Medicine Technology Biology Dictionary Medicine Dictionary. HOME >> BIOLOGY >> NEWS. Different microarray systems more alike than previously thought A multicenter comparison of equipment that can analyze the expression ...Published in the May 2005 issue of Nature Methods the study provides ... Biologists occasionally ask me what is the best platform on which to ...The impact of such a finding is not insignificant experts say becaus...For the study 10 laboratories in the Washington D.C./Baltimore area ... http://www.bio-medicine.org/inc/biomed/biology-news.asp http://feeds.bio-medicine.org/latest-biology-news. A multicenter comparison of equipment that can analyz...
http://bio-medicine.org/biology-news/Different-microarray-systems-more-alike-than-previously-thought-515-1/
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 3
Read more Articles Videos. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Porcine V2 Gene Expression Microarray, 4x44 Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rabbit Gene Expression Microarray, 4x44K Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Rice Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Sheep Gene Expression Microarray, 8x15k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides resea...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-3/?viewAll=1&pageSize=25&sortBy=r&u=1733685C-B6FB-48E5-9612-D958C9602799
*  Next Generation Sequencing Products, Reviews and Suppliers on SelectScience - page 2
Read more Articles Videos. DNA and RNA Purification. Compare or Request Pricing for Selected Product s Yeast Genome Microarray, 1x244k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Yeast Genome Microarray, 4x44K Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Arabidopsis V4 Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers with clear insight into species research through traditional chromatin immunop... Compare or Request Pricing for Selected Product s Canine V2 Gene Expression Microarray, 4x44k Agilent Technologies Agilent™s Model Organism ChIP-on-chip Microarrays provides researchers w...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/page-2/?viewAll=1&pageSize=100&sortBy=r&u=E619A817-CCE1-483F-A853-3EAA9ADE9774
*  JCVI: Use of RNA and Genomic DNA References for Inferred Comparisons In DNA Microarray Analyses
... Home. About. Research. Sustainable Lab. Publications. Education. Giving. Press. Careers. Contact. Listing. About. . Publications. Citation. Kim, H., Zhao, B., Snesrud, E. C., Haas, B. J., Town, C. D, Quackenbush, J. Use of RNA and Genomic DNA References for Inferred Comparisons In DNA Microarray Analyses. Biotechniques. 2002 Oct 01; 33 4 : 924-30. PubMed Citation. Abstract. In most microarray assays, labeled cDNA molecules derived from reference and query RNA samples are co-hybridized to probes arrayed on a glass surface. Gene expression profiles are then calculated for each gene based on the relative hybridization intensities measured between the two samples. The most commonly used reference samples are typically isolates from a single representative RNA source RNA-0 or pooled mixtures of RNA derived from a plurality of sources RNA-p. Genomic DNA offers an alternative reference nucleic acid with a number of potential advantages, including stability, reproducibility, and a potentially uniform representat...
http://jcvi.org/cms/publications/listing/abstract/article/use-of-rna-and-genomic-dna-references-for-inferred-comparisons-in-dna-microarray-analyses/
*  JN | Abstract | Microarray-based method for screening of immunogenic proteins from bacteria
JN. Abstract. Microarray-based method for screening of immunogenic proteins from bacteria. jnanobiotechnology.com/article/10.1186/1477/3155/10/12. Bottom,Top. BioMed Central. Journals. Gateways. . 4.12. Search JN BioMed Central for. Go. Advanced search. Home. Articles. Authors. Reviewers. About this journal. My JN. Journal of Nanobiotechnology. Volume 10. Viewing options. Abstract. Full text. PDF 2.2MB. Additional files. Associated material. PubMed record. Article metrics. Open Badges. Readers' comments. Related literature. Cited by. Google blog search Other articles by authors. on Google Scholar. Hoppe S. Bier FF. von Nickisch-Rosenegk M. on PubMed. Hoppe S. Bier FF. von Nickisch-Rosenegk M. Related articles/pages. on Google. on Google Scholar. on PubMed. Tools. Download references. Download XML. Order reprints. Post a comment. Download to ... Papers. Mendeley. Download to ... Papers. Mendeley. Share this article. Tweet. More options... Citeulike. LinkedIn. Del.icio.us. Email. ....
http://jnanobiotechnology.com/content/10/1/12/abstract
*  .. ChipDX Finds 163-Gene Signature that Predicts Early-Stage Colon Cancer Progres
Affymetrix microarray-based gene-expression technology was used. ChipDX reports the discovery and validation of a genetic signature for early-stage colon cancer. Currently, ChipDX is making the algorithm available for research use only through an online gene-expression analysis platform. ChipDX developed its prognostic algorithm by analyzing data from a 2009 study appearing in Gastroenterology of 232 U.S.-based colon cancer patients. Gene-expression data analysis was performed to identify genes significantly associated with recurrence independent of the patient s age at diagnosis, tumor grade, or disease stage. By combining measurements of these genes, performed with Affymetrix GeneChip technology, and a robust predictive algorithm, we were able to predict which individuals were at the greatest risk of recurrence within a five-year follow-up period. The studies in Gastroenterology and Clinical Cancer Research both utilized Affymetrix GeneChip Human Genome U133 Plus 2.0 Array profiles of colon cancer patients....
http://genengnews.com/keywordsandtools/print/4/21367/
*  Gene chip analysis
Data processing and quality control Replicates Normalization Quality control Filtering of flagged data Filtering of noisy replicates Filtering of non-significant genes Statistical analysis Clustering Hierarchical clustering K-means clustering Gene ontology studies Pathway analysis References. Data processing and quality control. Data processing includes data normalization, flagging of the data, averaging the intensity ratio for replicate s, clustering of similarly expressed genes, etc. Quality control. Filtering of noisy replicates. Filtering of noisy replicates is a crucial part of quality control. Filtering of non-significant genes. Filtering of non-significant genes is done so that analysis can be done on selected genes. Hierarchical clustering. Hierarchical clustering is a statistical method for finding relatively homogeneous clusters. After calculation of the initial distance matrix, the hierarchical clustering algorithm either A joins iteratively the two closest clusters starting from single data points...
https://en.wikipedia.org/wiki/Gene_chip_analysis
*  Microarray Probeset Mapping
... Ensembl Loading. BLAST/BLAT. BioMart. Tools. Downloads. Help Documentation. Blog. Mirrors. More. Search terms. Search all species. Search Ensembl genomes. Search Vega. Search EMBL-EBI. Search Sanger. Using. Annotation. Data. API. About. Using this website. Annotation and prediction. Data access. API software. About us. Select a species. In this section Ensembl stable IDs. Stable ID Version Increments in Ensembl. Gene Annotation. Genome Assemblies. 2x genomes. Genome Annotation. Ig/Tcr Segment Genes. Non-Coding RNAs. RNASeq Gene Models. The Consensus CDS Project. Variation. Data Description. Predicted Data. Detailed species data count. Import VCF script. Phenotype Sources. Variant Sources. Comparative Genomics. EPO Pipeline. Family and GeneTree stable ID. Gene Families. Protein trees and orthologies. Sequence-based analyses. Statistics on ncRNA trees. Statistics on protein trees. ncRNA trees and homologies. Microarray Probeset Mapping. Regulation. Regulatory Build. Regulation Sources. Regulatory Segmentat...
http://ensembl.org/info/genome/microarray_probe_set_mapping.html
*  microarray after overexpression and silencing - Macroarray and Microarray - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Forums. Forums. BioForum. Protocols and Techniques Forums. Macroarray and Microarray. Javascript Disabled Detected You currently have javascript disabled. microarray after overexpression and silencing Started by. Fahad, Oct 18 2011 05:03 AM microarray. Please log in to reply. 2 replies to this topic #1. My negative control was an empty vector amplified and purified in the same way. My negative control was an unspecific universal negative siRNA control. In both experiment, RNA was extracted for microarray analysis to explore which genes have been affected by the overexpression and the silencing. My questions: In case of the overexpression experiments, I noticed that the viability of cells transfected with the empty vector negative control was always reduced suggesting the presence of bacterial DNA contaminants. So, how can I exclude the unspeci...
http://protocol-online.org/forums/topic/23136-microarray-after-overexpression-and-silencing/
*  [BioC] remove microarray batch effects using Limma
remove microarray batch effects using Limma. remove microarray batch effects using Limma Xie, Zhi NIH/NHLBI zhi.xie at nih.gov. Mon Oct 25 20:36:35 CEST 2010. Previous message: rtracklayer -- how to hide some default tracks. fine-grained control of track view mode pack, full, dense, squish... Next message: remove microarray batch effects using Limma Messages sorted by:. Hi everyone, I have some microarray data files containing two sets of samples in normal and disease condition. I have tested that the data also contain significant batch effects with hybridization time. However, the positive hits I obtained using the following approaches are very different using the same cutoff value in decideTests function. I think I am supposed to use the first approach but I am surprised to see a big difference between the two approaches. could anyone help figure out the reasons. Thanks, Zhi Xie NIH/NHLBI Here eset is the expression dataset after RMA function. Approach 1: # Consider batch effects in the ...
https://stat.ethz.ch/pipermail/bioconductor/2010-October/036039.html
*  Oligonucleotide Arrays
... next cdna microarrays up dna chips microarrays previous oligo fingerprinting oligonucleotide arrays the basic idea in these chips is similar the difference is that these chips contain the oligos and not the targets the length of the oligos used depends on the application but they are usually no longer than bases since oligos are usually shorter the density is much higher in these chips for instance a chip that is cm by cm can easily contain oligos the chips are used in a similar manner they are exposed to a solution containing many copies of the target dna hybridization occurs between oligos and matching dna and then the chip can be heated in order to repeat the experiment figure shows a schematic description of such an experiment while the basic idea is the same the chips are obviously intended for different experiments this kind of chips is most useful in situations where we have relatively few targets but a large number of oligos we wish to test another advantage is that we may want to hybridize targe...
http://cs.tau.ac.il/~rshamir/algmb/00/scribe00/html/lec11/node10.html
*  Affymetrix Santa Clara California Reports Fourth Quarter and Fiscal Year 2012 Results
... Employer:. Login. Post Jobs. Job Seeker:. Login. Sign Up. Home. News. Jobs. Career Resources. Hotbeds. Career Fairs. Events. Company Profiles. Investors. Search Life Sciences Jobs. Search Job Title Only. Radius: Miles. Km 80.5. News. News By Subject. News by Disease. News By Date. Search News. Get Our FREE Industry eNewsletter email:. Affymetrix Santa Clara, California. AFFX Reports Fourth Quarter and Fiscal Year 2012 Results. Tweet. 2/1/2013 6:38:43 AM Affymetrix, Inc., NASDAQ: AFFX today reported its operating results for the three and twelve months ended December 31, 2012. Results for the three months ended December 31, 2012: • Total revenue was $84.4 million, which excluding eBioscience of $18.1 million, represented a 2% increase from the fourth quarter of 2011. Read at BioSpace.com. Related News Hamilton Robotics Builds Affymetrix Santa Clara, California AFFX ' CytoScan Automated Target Preparation Solution on Microlab NIMBUS. Pfizer Inc. PFE Q4 Profit Brings Plan to Reshape R&D Budget Back on Track...
http://biospace.com/News/affymetrix-santa-clara-california-reports-fourth/286374/source=MoreNews
*  www.genomebiology.com - Additional file 1
www genomebiology com additional file additional file supplemental figures s to s and supplemental table s figure s procedure and plant photographs of split root experiments figure s mycorrhization phenotype of wild type nts and nts in the split root experiments ii and iii figure s mycorrhization phenotype of wild type nts and nts in dependence on phosphate and nitrate fertilization figure s putative nark response genes identified by affymetrix genechip analysis figure s additional putative nark response genes in soybean plants days after inoculation figure s putative nark response genes in week old soybean plants figure s affymetrix gene expression data of putative annexins days after inoculation figure s sequence information of the putative ccaat binding tf genes targeted by gmaaffx s at figure s affymetrix gene expression data of other putative nf y genes in soybean plants days after inoculation figure s transcripts...
http://genomebiology.com/2013/14/6/R62/suppl/S1
*  .. Introduction .. Calendar
introduction biological arrays are nothing more than an ordered set of compounds affixed to a solid surface by applying a sample solution to the array it is possible to assay the interaction of the sample with each of the compounds on the array microarrays are a powerful research tool because they enable massively parallel assays of biological samples the most common application of microarrays is gene expression analysis in this case the interaction between labeled mrna in a biological sample and complementary dna probes which are affixed to the array allows rapid quantification of the entire transcriptome many other types of arrays are possible nucleic acid arrays can also be used for genotyping and antibody arrays can be used for proteomics the affymetrix genechip system http www affymetrix com products arrays index affx is a commercial platform that enables rapid reproducible and accurate microarray analysis on a genome wide scale the affymetrix genechip platform can be used for gene express...
http://bumc.bu.edu/microarray/introduction/
*  Target-Enrichment Field Continuing to Expand | GEN Magazine Articles | GEN
Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Page 2 of 2 Barcoded Samples We started off at a time when in solution target enrichment was not yet available, and relied, initially, on array-based enrichment, says Edwin Cuppen, Ph.D., professor of genome biology and human genetics at the Hubrecht Institute, KNAW. Gibbs, Ph.D., professor and director of the human genome sequencing center at Baylor College of Medicine, together with colleagues from Roche NimbleGen published one of the first reports on the use of solid-based hybridization based enrichment of human genomic regions by programmable custom high-density oligonucleotide microarrays. To expand the regions examined during target enrichment, the investigators included two new reagents. Gibbs and his team conducted the first genome-wide targeted capture analysis of a diverse set of biologically relevant genomic elements, and revealed decreased capture of variants ...
http://genengnews.com/gen-articles/target-enrichment-field-continuing-to-expand/3878/?kwrd=DNA&page=2
*  DNA Microarrays
... About Microbiology. About Microbiology What is a Microbe. Types of Microbes. Interesting Facts. Careers in Microbiology. Types of Careers. How to Get Started in Microbiology. Tools of the Trade. DNA Microarrays. History of Microbiology. Podcasts & Videos. Microbes After Hours. Click for more " Microbes After Hours " videos. Careers in Microbiology. Tools of the Trade. DNA Microarrays. DNA Microarrays. If a microbiologist is studying bacteria that bioremediate, or break down, toxic wastes and wants to know which specific genes are active when that bacterium is degrading, say, PCBs, he would likely use a tool called the DNA microarray. All microarrays also called DNA chips or gene chips work on the basic principle that complementary nucleotide sequences in DNA and RNA match up like the two halves of a piece of Velcro coming together. A bacterium’s entire genetic make-up can be contained on a single gene chip. There can be thousands or tens of thousands of these tiny spots on a single slide. He would first ...
http://microbeworld.org/careers/tools-of-the-trade/genetic-tools-and-techniques/dna-microarrays
*  DNA Chips Provide Key to Human Genome Analysis | GEN Magazine Articles | GEN
DNA Chips Provide Key to Human Genome Analysis. FDA Nominee Driven by "Data, Data, Data". FDA Nominee Driven by "Data, Data, Data". Market & Tech An... DNA Chips Provide Key to Human Genome Analysis Microarray Market Is Growing as Applications for Diagnostics and Research Are Realized Nina Flanagan. Although DNA microarrays are just beginning to be investigated for diagnostics, they are becoming key tools for preclinical and clinical research. Gene-expression analysis continues to provide information on what is occurring on a molecular level and is crucial to discovering new drug candidates. “We try to fuse the advantage of the microarray being a multiplex analysis with the high data quality of the PCR reaction,” explained Staehler. There are currently two kits available, the ULS Small RNA labeling kit and the miRACULS miRNA kit. Streamlined Genomic Labeling Invitrogen www.invitrogen.com recently launched the BioPrime® Total Genomic DNA Labeling System for use in array comparative genomic hybridization applic...
http://genengnews.com/gen-articles/dna-chips-provide-key-to-human-genome-analysis/2140/
*  Array Techniques
... prev next top macro language guide variables array techniques the source insight macro language does not support array variables however file buffers can be used as dynamic arrays each line in a buffer can represent an array element furthermore record variables can be stored in each line to give you record arrays buffer functions are described in a following section some useful functions are newbuf and closebuf for creating and destroying buffers and the buffer line functions getbufline putbufline insbufline appendbufline delbufline and getbuflinecount you can also call newwnd to put the array buffer in a window so you can see the array contents this example creates a buffer array of records hbuf newbuf rec name joe smith age experience guru appendbufline hbuf rec rec name mary x age experience intern appendbufline hbuf rec hbuf now has records in it rec getbufline hbuf retrieve th element closebuf hbuf in this section the if statement the while statement break and continue conditional evaluation prev ne...
http://sourceinsight.com/docs35/ag922220.htm
*  Validation of microarray data by qPCR - PCR, RT-PCR and Real-Time PCR - BioForum
... Google Sign in options. Remember me This is not recommended for shared computers Sign in anonymously Don't add me to the active users list Privacy Policy. Sign In. Forums. BioWiki. Quotes. BioVideo. Forums. BioWiki. Quotes. Contact Us. BioForum. Protocols and Techniques Forums. PCR, RT-PCR and Real-Time PCR. Javascript Disabled Detected You currently have javascript disabled. Validation of microarray data by qPCR Started by. vértigo, Jan 11 2012 03:12 AM selecting genes. qPCR. 1 reply to this topic #1. vértigo. vértigo. 1 posts. Posted 11 January 2012 - 03:12 AM Hi! I have to validate my microarray data performed in Affymetrix chips by qPCR SYB green and I have some questions about selecting genes. How many genes do I need to validate. So, do I also need to perform qPCR for down-expressed genes. Is there any biological or technical difference regarding validation between up- and down-regulated genes. I ve found in literature that array and qPCR have lower correlation for down-expressed genes because ...
http://protocol-online.org/forums/topic/23977-validation-of-microarray-data-by-qpcr/
*  NCI Assists Establishment of DNA Microarray Facilities - 09/21/1999
... NATIONAL INSTITUTES OF HEALTH. National Cancer Institute. FOR IMMEDIATE RELEASE Tuesday, September 21, 1999. Contact: NCI Press Office 301 496-6641. NCI Assists Establishment of DNA Microarray Facilities. The National Cancer Institute NCI announced today that it will spend $4.1 million to help 24 cancer research centers throughout the country purchase equipment needed to launch DNA microarray facilities. DNA microarray technology is a powerful, but technically challenging, new research tool that allows scientists to assess the level of expression of a large subset of the 100,000 human genes in a cell or tissue. The new facilities will offer state-of-the-art technological support to scientists who study the molecular causes of cancer. They also will help to ensure that this important new technology is broadly available to the cancer research community, and that the technology's tremendous potential is rapidly realized by ensuring that it is applied to a broad spectrum of critical problems in cancer resear...
http://nih.gov/news/pr/sept99/nci-21.htm
*  Microarray Technology Moves to the Fore | GEN Magazine Articles | GEN
Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. FDA Nominee Driven by "Data, Data, Data". More GEN Exclusives ». Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. FDA Nominee Driven by "Data, Data, Data". More GEN Exclusives ». Market & Tech Analysis. GEN Exclusives More. Page 1 of 3 Microarray technology has come of age as a powerful approach to revealing the genome and its dynamic expression through the application of gene-hybridization or gene-expression microarrays. Because gene-expression microarrays display the transcriptional activity of the tissue from which they are derived, they have the potential to reveal new and unanticipated gene functions, including unforeseen disease subtypes, new diagnostic markers, and mechanisms determining disease susceptibility. Almac Group, a Powered by Affymetrix partner, enables the transfer of biomarkers from research to the clinic with proprietary arrays using Affymetrix microarray technology, including the Xcel™ for biomarke...
http://genengnews.com/gen-articles/microarray-technology-moves-to-the-fore/3463/
*  GeneChip® Mouse Genome 430 2.0 Array | AffymetrixCatalogNav
GeneChip Mouse Genome 430 2.0 Array. Affymetrix. Quick Order. 0 Items. Welcome, Guest. Login. Register. Site. Product Catalog. Quick Order. jp cn. CatalogNav Home. Products. Products. Products Listed A-Z. Promotions. Brands. Affymetrix Microarray Brands. USB Molecular Biology Reagents. eBioscience Immunology Reagents. Community. Scientific Communities. Researchers. Support. Affymetrix Microarray Solutions. USB. Partners Programs. Overview. OwnerChip Program. GeneChip-compatible Software Providers. Service Providers. Developers' Network. Consortia Program. Core Lab Program. Powered by Affymetrix Partners. About Affymetrix. Overview. News & Events. Investors. Corporate Governance. Contact Us. Careers. Overview. Job Search. NetAffx. Expression Array Comparison Tool. NetAffx Analysis Center. Products Microarray Solutions Microarray Solutions View all DNA Analysis Solutions Genome-Wide Genotyping for Human Disease Research. Targeted Genotyping Analysis for Human Disease Research. Agrigenomics Solutions. Mo...
http://affymetrix.com/estore/browse/products.jsp?navMode=34000&productId=131477&navAction=jump&aId=productsNav
*  .. All Entries Tagged With: "custom microarray" .. microRNA Profiling Using a High Performance, Fl
all entries tagged with custom microarray microrna profiling using a high performance flexible âµparafloâ biochip platform abstract an advanced microfluidic biochip system designed to produce high quality data stay current with the rapidly evolving microrna field and perform diverse small rna discovery experiments is presented this technology s unique flexibility allows for mirbase synchronicity and design of customized biochips adapted to each researcher s specific needs applications featuring disease marker discovery drug treatment admin jan comments full story categories bio mems lab on a chip mems microfabrication microfluidics nanotechnology optical mems moems sensors actuators system on chip documentaries events featured mems help archives select month october september august may april march february january december october july june may april march february january december november october september august july june may april march february january december november october september august july jun...
http://memsuniverse.com/tag/custom-microarray
*  Result Content View
... Frequency of Chromosome 3 LOH and Partial Loss in Uveal Melanoma by Genomic Microarray Analysis of Frozen Tissue Eugen C Minca, Raymond R Tubbs, Bryce P Portier, Zhen Wang, Christopher Lanigan, Charles Biscotti, Mary Beth Aronow, Pierre Triozzi, Arun Singh, James R Cook, Yogen Saunthararajah, Lynn Schoenfield, Blake C Baliff, Roger A Schultz. Cleveland Clinic Foundation, Cleveland, OH; Signature Genomics, Spokane, WA Background: Molecular cytogenetic alterations, especially monosomy of chromosome 3, are strongly correlated with metastases and death in uveal melanoma UVM. Here we assessed the frequency of chromosome 3 LOH and partial loss by combined genomic and SNP array analysis of DNA extracted from frozen tissue FZT procured from a consecutive series of enucleations performed for UVM. DNA was fluorescently labeled Roche and microarray analysis was performed using a custom designed Roche NimbleGen array OncoChip v2 with oligonucleotide coverage biased to >2400 cancer features and backbone SNP coverage ...
http://abstracts2view.com/uscap13/view.php?nu=USCAP13L_1755
*  [BioC] Help with Oligo Package: Extracting and Graphing
Help with Oligo Package: Extracting and Graphing. Help with Oligo Package: Extracting and Graphing Tobias Straub tstraub at med.uni-muenchen.de. Wed Nov 11 15:08:09 CET 2009. Previous message: Help with Oligo Package: Extracting and Graphing Next message: How to save the RMA result in .RData file oligo package. Messages sorted by:. Hi Noah, did you contact Benedikt, the author of the Starr package. the department he's working in is performing tiling array analysis in S. cerevisiae most likely on exactly the same array as yours and i am pretty sure they can provide not only the proper pd file but also a 'working' gff file. i guess that the Starr package would provide most of the functions you are asking for. best t. On Nov 10, 2009, at 8:39 PM, Noah Dowell wrote: Hello All, I am completely new to using Bioconductor and R so please excuse the simplistic questions. I have combed through this message board and the general web for help over the past several weeks before posting, but I may have missed the answers t...
https://stat.ethz.ch/pipermail/bioconductor/2009-November/030461.html
*  Cytogenetic Research Only Products | PerkinElmer
Cytogenetic Research Only Products. PerkinElmer. Certificate of Analysis. Reagents. Products. Assays Reagents. Alpha Technology. Clinical Diagnostics Reagents. Clinical MSMS Reagents. Cytogenetic Diagnostic Products. Cytogenetic Research Only Products. In Vivo Imaging Reagents. Maternal Fetal Health Reagents. Newborn Screening Reagents. Chromatography Systems. Elemental Analysis. Food Test Kits. Labeling & Detection. Liquid Handling. Live Cell Imaging. Microplates. Molecular Spectrometers. Newborn Screening Systems. Preclinical In Vivo Imaging Accessories. Prenatal Screening Systems. Sample Preparation. Thermal Analysis. Automation & Liquid Handling. Chromatography Systems. Elemental Analysis. High Content Screening. In Vivo Imaging & Analysis. Molecular Spectroscopy. Newborn Screening Systems. Prenatal Screening Systems. Quantitative Pathology Imaging & Analysis. Scintillation & Gamma Counters. Thermal Analysis. Cellular Imaging Analysis. In Vivo Imaging Software. Dedicated to bringing the newest molecular t...
http://perkinelmer.com/catalog/category/id/cytogenetics products for research
*  www.molecularcytogenetics.org - Figure
www molecularcytogenetics org figure resolution standard high figure oligonucleotide microarray analysis of a mosaic q deletion shaded blue region the zoomed in microarray plot shows a single copy loss of probes from q approximately mb in size chr based on ucsc hg assembly probes are ordered on the x axis according to physical mapping positions with the most proximal q probes to the left and the most distal q probes to the right neill et al molecular cytogenetics doi download authors original image...
http://molecularcytogenetics.org/content/3/1/11/figure/F2
*  /gnuradio-core/src/lib - Repository - GNU Radio - gnuradio.org
... Sign in Register. Home Projects Help. Search :. Overview Activity Roadmap Issues News Wiki Files Repository. Statistics. Branch:. android maint master next Tag:. 3.3git 3.5.1git 3.5.2git 3.5git 3.6.0git 3.6.1git 3.6.2git 3.6.3git 3.6git 3.7.0git 3.7.2git 3.7.3git 3.7.5git 3.7.6git 3.7.7git 3.7.8git 3.7.9git 3.7git distcheck-master maint-3.3.0 maint-3.4.0 maint-3.4.1 maint-3.4.2 maint-3.5.1 maint-3.5.2 maint-3.5.3 maint-3.6.0 maint-3.6.1 maint-3.6.2 v3.3.0 v3.3.0-rc0 v3.3.0-rc1 v3.3.0-rc2 v3.3.0-rc3 v3.3.1 v3.3.1git v3.4.0 v3.4.0git v3.4.1 v3.4.1git v3.4.2 v3.4.2git v3.4git v3.5.0 v3.5.0git v3.5.0rc0 v3.5.1 v3.5.2 v3.5.2.1 v3.5.3 v3.5.3.1 v3.5.3.2 v3.5.3git v3.6.0 v3.6.1 v3.6.2 v3.6.3 v3.6.3.1 v3.6.3rc0 v3.6.4 v3.6.4.1 v3.6.4.2 v3.6.4git v3.6.5 v3.6.5.1 v3.7.0 v3.7.0.1 v3.7.0rc0 v3.7.1 v3.7.1.1 v3.7.2 v3.7.2.1 v3.7.2.2 v3.7.3 v3.7.4 v3.7.4.1 v3.7.4git v3.7.5 v3.7.5.1 v3.7.5.2 v3.7.6 v3.7.6.1 v3.7.6.2 v3.7.7 v3.7.7.1 v3.7.7.2 v3.7.8 v3.7.8rc1 Revision:. gnuradio / gnuradio-core / src / lib @ 0cbaf1b3. Name...
http://gnuradio.org/redmine/projects/gnuradio/repository/revisions/0cbaf1b350c5ef6fc25f429430f1c892e7844e01/show/gnuradio-core/src/lib
*  PHP: array pop - Manual
array pop PHP 4, PHP 5 array pop Liefert und entfernt das letzte Element eines Arrays. array pop. array. function array trim end $array { $num=count $array ; $num=$num-1; unset $array ; return $array; }. $arrayOfArrays ; foreach $compareArray AS $key = $valueArray { foreach $arrayOfArrays AS $subArray = $contents { if. $compareArray, $contents { unset $compareArray ; } } } return $compareArray ; }. array shift requires a re-index process on the array, so it has to run over all the elements and index them. It cuts the given index value off of the array, but without the shift, if the 'index' value isn't given, it cuts off the end value. Hi, Here is a simple function which delete one element from the array with value : ?php /* * This function deletes the given element from a one-dimension array * Parameters: $array: the array in/out * $deleteIt: the value which we would like to delete * $useOldKeys: if it is false then the function will re-index the array from 0, 1, ... * if it is true: the function will keep th...
http://php.net/manual/de/function.array-pop.php
*  PHP: Arrays - Manual
A map is a type that associates values to keys. ?php $array = array "foo" = "bar", "bar" = "foo", ; // as of PHP 5.4 $array = ;. PHP arrays can contain integer and string keys at the same time as PHP does not distinguish between indexed and associative arrays. ?php $array = array "foo" = "bar", "bar" = "foo", 100 = - 100, - 100 = 100, ; var dump. ?php $array = array "foo", "bar", "hello", "world" ; var dump. array 4 { = string 3 foo = string 3 bar = string 5 hello = string 5 world }. "\n" ; echo "Bad: { $array } \n" ; echo "Good: { $array } \n" ; }. " ; // Hello apple // With one exception: braces surrounding arrays within strings allows constants // to be interpreted print "Hello { $arr } " ; // Hello carrot print "Hello { $arr } " ; // Hello apple // This will not work, and will result in a parse error, such as: // Parse error: parse error, expecting T STRING' or T VARIABLE' or T NUM STRING' // This of course applies to using superglobals in strings as well print "Hello $arr " ; print "Hello $ GET " ; // Co...
https://secure.php.net/manual/it/language.types.array.php
*  BioMicroCenter:News - OpenWetWare
... BioMicroCenter:News From OpenWetWare Difference between revisions Jump to: navigation, search Revision as of 22:44, 7 January 2009 view source Bill Flanagan Talk. contribs → Previous Newsletters ← Previous diff. Revision as of 22:49, 7 January 2009 view source Bill Flanagan Talk. contribs → PREVIOUS NEWSLETTERS Next diff →. Line 22:. Line 22:. - - {{BioMicroCenter:News/2008/Content}}. {{BioMicroCenter:News/2008/Content}}. - !--. + !-- Remove Comments when Jan 2009 Newsletter is ready. - - ' ]'. ' ]'. - - {{BioMicroCenter:News/2009/Content}}. {{BioMicroCenter:News/2009/Content}}. - --. + Remove Comments when Jan 2009 Newsletter is ready --. } } } } Revision as of 22:49, 7 January 2009. BIOMICRO News Staff Research Internal Site GENOMICS Illumina Sequencing Sample Prep Microarrays Chromatin IP HIGH-THROUGHPUT Robotics RT-PCR BioAnalyzer Advanced Analytical Plate Reading COMPUTING Bioinformatics Software Computational Resources TOOLS Resources Protocols The Idea Center QUICK LINKS FAQ Sample Submission Form...
http://openwetware.org/index.php?title=BioMicroCenter:News&diff=275733&oldid=275724
*  Arrays - HaskellWiki
... Personal tools Home. Log in. Views Page. Discussion. View source. History. Arrays From HaskellWiki Revision as of 14:34, 20 May 2006 by Bulatz. Talk. contribs. diff ← Older revision. Latest revision diff. Newer revision → diff. Jump to: navigation, search Haskell'98 supports just one array constructor type, namely Array, which gives you immutable boxed arrays. "Immutable" means that these arrays, like any other pure functional data structure, have contents fixed at construction time. You can't modify them, only query. There are "modification" operations, but they just return new array and don't modify the original one. This makes possible to use Arrays in pure functional code along with lists. "Boxed" means that array elements are just ordinary Haskell lazy values, which are evaluated on demand, and can even contain bottom undefined value. You can learn how to use these arrays at http://haskell.org/tutorial/arrays.html and I recommend that you read this before proceeding to rest of this page. Nowadays ma...
https://wiki.haskell.org/index.php?title=Arrays&oldid=4142
*  Cellular microarray
The support, sometimes called a "chip", is spotted with varying materials, such as antibodies, proteins, or lipids, which can interact with the cells, leading to their capture on specific spots. Combinations of different materials can be spotted in a given area, allowing not only cellular capture, when a specific interaction exists, but also the triggering of a cellular response, change in phenotype, or detection of a response from the cell, such as a specific secreted factor. Reverse transfection cell microarrays. Sabatini's laboratory developed reverse-transfection cell microarrays at the Whitehead Institute, publishing their work in 2001. Chen DS, Davis MM 2006 Molecular and functional analysis using live cell microarrays. Curr Opin Chem Biol 10:28-34 Chen DS, Soen Y, Stuge TB, Lee PP, Weber JS, Brown PO, Davis MM 2005 Marked Differences in Human Melanoma Antigen-Specific T Cell Responsiveness after Vaccination Using a Functional Microarray. PLoS Med 2: 10: e265 http://medicine.plosjournals.org/perlserv/?r...
https://en.wikipedia.org/wiki/Cellular_microarray
*  Data.Array.Vector
Array classes The pure and mutable array types Streaming pure arrays Conversions to/from lists Basic operations on pure arrays Introducing and eliminating UArrs Basic interface Transforming UArrs Reducing UArr s folds. Logical operations Arithmetic operations Building UArrs Scans Accumulating UArrs Generating UArrs Subarrays Breaking arrays Searching Arrays Searching by equality Searching with a predicate Indexing UArr Zipping and unzipping Enumerations Low level conversions Low level conversions Copying arrays Packing CStrings and pointers Using UArrs as CStrings I/O with UArr s Permutations Searching Arrays of pairs Random arrays I/O Operations on mutable arrays Strict pairs and sums Injection and projection functions Currying Strict Maybe Lazy wrapper Class of hyperstrict types Description. data MUArr e :: * - *. class UA a = UIO a where. Basic operations on representation types ----------------------------------------- This type class determines the types that can be elements immutable unboxed arrays. Yie...
http://hackage.haskell.org/package/uvector-0.1.0.3/docs/Data-Array-Vector.html
*  c++ - Difference between std::tr1::array and boost::array - Stack Overflow
... Stack Overflow. Meta Stack Overflow. Stack Overflow Careers. Stack Exchange. stack overflow careers. Stack Overflow Questions. Stack Overflow is a question and answer site for professional and enthusiast programmers. Difference between std::tr1::array and boost::array. I was under the impression that std::tr1::array was the same as the boost::array in that it would throw an exception when accessing an index out of bounds. #include exception #include iostream #include string #include tr1/array #include boost/array.hpp int main { // boost::array std::string, 3 arr; std::tr1::array std::string, 3 arr; try { arr.at 0 = "one"; arr.at 1 = "two"; arr.at 2 = "three"; arr.at 3 = "nogood"; } catch const std::exception e { std::cout "exception: " e.what std::endl; } return 0; }. c++ boost arrays std tr1 share. Here's what GCC does for your code on my system Linux x86-64 : $ g++-4.1.2 test.cpp -o test $ ./test exception: array:: M at $ g++-4.3.5 test.cpp -o test $ ./test exception: array::at $ g++-4.4.4 test.cpp -o ...
http://stackoverflow.com/questions/3101544/difference-between-stdtr1array-and-boostarray
*  Fujifilm develops the micro-needle array
... News and Press Release Distribution, Since 1995. Deliver Your News to the World. Sign In Create a Free Account. Home. News. How We Work. Compare Services. FAQ. All. WebPost. WebPost PPC. WebRelease. Targeted Media. Wire Service. Fujifilm develops the micro-needle array. WEBWIRE. Wednesday, November 14, 2012 A new method for delivering the drug into the body just by attaching it onto the skin Also enables mass production of arrays with projections of different length and form FUJIFILM Corporation President: Shigehiro Nakajima has developed the micro-needle array which is a new drug administration method that has gained attention for delivering the drug into the body just by attaching it onto the skin. A micro-needle array is a sheet arrayed with projections of 100-2000 micrometers. When the sheet is placed on the skin surface, the drug is injected into the skin from the projections and delivered into the body. The penetration of the projections of a micro-needle array will not cause pain like an injection...
http://webwire.com/ViewPressRel.asp?aId=164902
*  Data.Array.Repa.Repr.ByteString
... Source. Contents. Index repa-3.1.4.2: High performance, regular, shape polymorphic parallel arrays. Safe Haskell Safe-Infered. Data.Array.Repa.Repr.ByteString. Synopsis. data B. data family Array r sh e. fromByteString :: Shape sh = sh - ByteString - Array B sh Word8. toByteString :: Array B sh Word8 - ByteString. Documentation. data B Source. Strict ByteStrings arrays are represented as ForeignPtr buffers of Word8. Instances. Repr B Word8. Read elements from a ByteString. Combine B Word8 D b. Read sh = Read Array B sh Word8. Show sh = Show Array B sh Word8. data family Array r sh e Source. Arrays with a representation tag, shape, and element type. Use one of the type tags like D, U and so on for r, one of DIM1, DIM2 ... for sh. fromByteString :: Shape sh = sh - ByteString - Array B sh Word8 Source. O 1. Wrap a ByteString as an array. toByteString :: Array B sh Word8 - ByteString Source. O 1. Unpack a ByteString from an array. Produced by Haddock version 2.10.0....
http://hackage.haskell.org/package/repa-3.1.4.2/docs/Data-Array-Repa-Repr-ByteString.html
*  JCVI: Assessing Unmodified 70-mer Oligonucleotide Probe Performance on Glass-slide Microarrays
... Publications. Education. Careers. Listing. Publications. Citation. Wang, H. Y., Malek, R. S., Luu, T. V., Behbahani, B., Frank, B., Quackenbush, J., Lee, N. H Assessing Unmodified 70-mer Oligonucleotide Probe Performance on Glass-slide Microarrays. Genome Biol. 2003 Jan 16; 4 1 : R5. PubMed Citation. Abstract. BACKGROUND: Long oligonucleotide microarrays are potentially more cost- and management-efficient than cDNA microarrays, but there is little information on the relative performance of these two probe types. The feasibility of using unmodified oligonucleotides to accurately measure changes in gene expression is also unclear. RESULTS: Unmodified sense and antisense 70-mer oligonucleotides representing 75 known rat genes and 10 Arabidopsis control genes were synthesized, printed and UV cross-linked onto glass slides. Printed alongside were PCR-amplified cDNA clones corresponding to the same genes, enabling us to compare the two probe types simultaneously. Hybridization signal intensity did not correlat...
http://jcvi.org/cms/publications/listing/abstract/article/assessing-unmodified-70-mer-oligonucleotide-probe-performance-on-glass-slide-microarrays/
*  Molecular beacon
thumb|Structure of molecular beacons in their native conformations top or hybridized with a DNA strand bottom 'Molecular beacons' are oligonucleotide hybridization probe s that can report the presence of specific nucleic acid s in homogenous solutions. The term more often used is 'molecular beacon probes'. Molecular beacon probes Synthesis Alternative homogeneous assay technologies Applications of molecular beacons References. Molecular beacon probes. The middle 15 nucleotides are complementary to the target DNA or RNA and do not base pair with one another, while the five nucleotides at each terminus are complementary to each other rather than to the target DNA. A typical molecular beacon structure can be divided in 4 parts: 1 loop, an 18–30 base pair region of the molecular beacon that is complementary to the target sequence; 2 stem formed by the attachment to both termini of the loop of two short 5 to 7 nucleotide residues oligonucleotides that are complementary to each other; 3 5' fluorophore at the 5' end...
https://en.wikipedia.org/wiki/Molecular_beacon
*  Allele-specific oligonucleotide
... An 'allele-specific oligonucleotide ASO ' is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. These probes can usually be designed to detect a difference of as little as 1 base in the target's genetic sequence, a basic ability in the assay of single-nucleotide polymorphism s SNPs, important in genotype analysis and the Human Genome Project. To test for the presence of the mutation in a DNA sample, an ASO probe would be synthesized to be complementary to the altered sequence, Studencki AB, Conner BJ, Impraim CC, Teplitz RL, and Wallace RB "Discrimination among the human beta A, beta S, and beta C-globin genes using allele-specific oligonucleotide hybridization probes." Am J Hum Genet vol. Each ASO is fully complementary to its target sequence and will bind strongly, but has a single mismatch against its non-target allele leading to weaker interaction. Schematic of dot-blots using the "A" or "S" ASO probes. A segment of the beta-hemoglobin genes in the sample DNA s wou...
https://en.wikipedia.org/wiki/Allele-specific_oligonucleotide
*  ARS | Publication request: MOLECULAR BEACONS TO SELECT FOR SCN RESISTANCE AT RHG1 AND RHG4 ARS : Res
ARS. Publication request: MOLECULAR BEACONS TO SELECT FOR SCN RESISTANCE AT RHG1 AND RHG4 ARS : Research. ARS Home. About ARS. Search for This. National Programs. International Programs. Trending Research Topics. Scientific Software/Models. Find an office at headquarters. Careers. Careers at ARS Info. Site Map. Related Topics Programs and Projects. ARS National Programs. ARS Office of International Research Programs. ARS Office of International Research Programs Regional Contacts. ARS International Research Partnerships. Title: MOLECULAR BEACONS TO SELECT FOR SCN RESISTANCE AT RHG1 AND RHG4 Authors. Arelli, P - ARS USDA-ARS TENN. Submitted to: Plant and Animal Genome Conference Proceedings Publication Type: Abstract Only Publication Acceptance Date: December 20, 2002 Publication Date: N/A. Single nucleotide polymorphisms SNPs were identified that are closely associated with rhg1 and rhg4, two important loci controlling resistance to SCN. In this study we examine the use of molecular beacons and locked nucleic...
http://ars.usda.gov/research/publications/publications.htm?seq_no_115=142273
*  Federal probe targets halal food supplier in Iowa | Newton Daily News
Federal probe targets halal food supplier in Iowa. Newton Daily News. . Subscribe to Newton Daily News. News. News Home. Local. State. Nation and World. Sports. Sports Home. Local. Professional. Chicago Football. Records. Records Home. Obituaries. Police Reports. Corrections. Jasper Life. Opinion. Opinion Home. Guest Columnists. Editorials. Letters. Columnists. Lifestyle. Lifestyle. Food. Home Garden. Tech. Events. Upcoming Events. Submit Your Event. Video. News Video. Live Video. Share. . Birth Announcements. Birthday Announcements. Campus Report. Engagement Announcement. Press Release. Letters to the Editor. Subscribe to the Newton Daily News. Temporary Subscription Stop. Wedding Announcement. More. About Us. Business Showcase. Contact. Coupons. Daily Deals. e-Edition. Follow Us. Forms. Place an Ad. Printing. Special Sections. Subscribe. Subscriber Services. Text Alerts. Today's Ads. Weather. News. Sports. Records. Opinion. Lifestyle. Events. Video. Photos. Contests. Share. More. TextAlerts. Classified. Job...
http://newtondailynews.com/2012/11/26/federal-probe-targets-halal-food-supplier-in-iowa/aviuxql/
*  probeBase
... newsletter Get a list of all new probes when probeBase has been updated. probeBase comprises currently 2788 probes, 175 primer, 16 microarrays, and 499 references. Search Search probeBase for target organisms, probe names, primers, microarrays, target sites, probe accession numbers, references, etc. Match Match your 16S rRNA sequence s against probeBase and find all published probes targeting your sequence s. Lists View lists of probes according to probe categories, coverage of group-specific probes, microarrays, references, etc. Submission Submit new or missing probes to probeBase. Evaluation of primer pairs using probeBase and SILVA TestPrime Direct submission of primer sequences from probeBase to the. SILVA TestPrime tool facilitates evaluation of specificity and coverage of primer combinations. probeCheck uses an extendable list of established sequence databases including the rRNA databases Silva, RDP-II, and Greengenes, and the functional gene databases of FGPR. help-volv2 Rickettsial endosymbionts ...
http://131.130.66.201/probebase/
*  Xmap
... xmind workbook file extension xmap xmap multi analyte profiling technology is a multiplex assay format patented by luminex corporation using a panel of microspheres internally doped with two fluorescent dyes to produce up to different bead identities that can be used in various applications from hla genotyping by oligonucleotide probe hybridization to serological profiling e g antibody diversity profiling antibody identification or histocompatibility screen etc...
https://en.wikipedia.org/wiki/Xmap
*  Re: how abt ao-40 beacon on mode-S?
re how abt ao beacon on mode s date index thread index author index re how abt ao beacon on mode s subject re how abt ao beacon on mode s from achim vollhardt avollhar xxxxxxxxxxxxxxx date wed nov hi folks seems the mode s beacon for testing idea wasn t so bad at all live telemetry ut announces the mode s general beacon to be turned on between ma and ma so anybody with mode s equip start listening but beware of doppler which will be huge at ma calculations for my qth say about khz down the announced mhz happy hunting s achim dh va via the amsat bb mailing list at amsat org courtesy of amsat na to unsubscribe send unsubscribe amsat bb to majordomo amsat org follow ups re how abt ao beacon on mode s from jon ogden prev by date what about doppler correction next by date re how abt ao beacon on mode s prev by thread re how abt ao beacon on mode s next by thread re how abt ao beacon on mode s index es date thread amsat home...
http://amsat.org/amsat/archive/amsat-bb/200011/msg01546.html
*  Gene expression profiling reveals early cellular responses to intracellular magnetic labeling with s
... uperparamagnetic iron oxide nanoparticles. - PubMed - NCBI Warning: The NCBI web site requires JavaScript to function. NCBI. BLAST Basic Local Alignment Search Tool. BLAST Stand-alone. BLAST Basic Local Alignment Search Tool. BLAST Stand-alone. Conserved Domain Search Service CD Search. Conserved Domain Search Service CD Search. All Genes Expression Resources... PubMed. PubMed Central PMC. Search database All Databases Assembly BioProject BioSample BioSystems Books ClinVar Clone Conserved Domains dbGaP dbVar Epigenomics EST Gene Genome GEO DataSets GEO Profiles GSS GTR HomoloGene MedGen MeSH NCBI Web Site NLM Catalog Nucleotide OMIM PMC PopSet Probe Protein Protein Clusters PubChem BioAssay PubChem Compound PubChem Substance PubMed PubMed Health SNP SRA Structure Taxonomy ToolKit ToolKitAll ToolKitBook UniGene Search term. 1 selected item: 20373404 Format Summary Summary text Abstract Abstract text MEDLINE XML PMID List. Gene expression profiling reveals early cellular responses to intracellular magnetic ...
http://ncbi.nlm.nih.gov/pubmed/20373404
*  High Throughput Gene Expression/Biomarker Core Laboratory | UMASS Medical School
High Throughput Gene Expression/Biomarker Core Laboratory. UMASS Medical School. Main Menu Home Page. Meet the Staff. Instrumentation. Possible Applications. Sample Submission. Rates. Why This Core. Utility Links. Open/Close Menu. Search. Advanced Search. Search. Advanced Search. HT Gene Expression. Skip Navigation. Home Page. Meet the Staff. Instrumentation. Possible Applications. Sample Submission. Rates. Why This Core. . Contact Us Co-Director: Kahraman Tanriverdi, PhD Phone: 508-856-6962 kahraman.tanriverdi@umassmed.edu www.umassmed.edu/htcore. High Throughput Gene Expression/Biomarker Core Laboratory. The High Throughput Gene Expression/Biomarker Core Laboratory run by Drs. Tanriverdi and Freedman provides High Throughput/Rapid Gene Expression and Complete miRNA profiling analysis by using gold standard qRT-PCR with the combination of custom Integrated Fluidic Circuit IFC technology from Fluidigm South San Francisco, CA. Also, high-throughput SNP and multi-plate immunoassay services are available. The co...
http://umassmed.edu/htcore
*  Gene expression profiling reveals reproducible human lung adenocarcinoma subtypes in multiple indepe
... ndent patient cohorts. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. News & Publications:Scientific Publications Gene expression profiling reveals reproducible human lung adenocarcinoma subtypes in multiple independent patient cohorts. Read More / View Supplemental Materials. Scientific Publications Back to Publications Gene expression profiling reveals reproducible human lung adenocarcinoma subtypes in multiple independent patient cohorts. Tumor subtypes were distinguishable by many hundreds of genes, and lists generated in one cohort were predictive of tumor subtypes in the two other cohorts. Most notably, bronchioid tumors were correlated with improved survival in early-stage disease, whereas sq...
http://broadinstitute.org/publications/broad837
*  Genome-wide gene expression profiling and a forward genetic screen show that differential expression
... of the sodium ion transporter Ena21 contributes to the differential tolerance of Candida albicans and Candida dubliniensis to osmotic stress. . Home. Submit. Login. View Item. TARA. School of Dental Sciences. Dental Science. Dental Science Scholarly Publications. View Item. TARA. School of Dental Sciences. Dental Science. Dental Science Scholarly Publications View Item. JavaScript is disabled for your browser. Some features of this site may not work without it. Search TARA. This Collection. Advanced Search. Browse. All of TARA. Academic/Research Units Collections Titles Authors Date of Publication Subjects Sponsor This Collection. Titles Authors Date of Publication Subjects Sponsor. Statistics. View Google Analytics Statistics. Genome-wide gene expression profiling and a forward genetic screen show that differential expression of the sodium ion transporter Ena21 contributes to the differential tolerance of Candida albicans and Candida dubliniensis to osmotic stress. File Type:. PDF. Item Type:. Journal A...
http://tara.tcd.ie/xmlui/handle/2262/29626
*  ARS | Publication request: WHOLE-GENOME EXPRESSION PROFILING DEFINES THE HRPL REGULON OF PSEUDOMONAS
Publication request: WHOLE-GENOME EXPRESSION PROFILING DEFINES THE HRPL REGULON OF PSEUDOMONAS SYRINGAE PV. TOMATO DC3000, ALLOWS DE NOVO RECONSTRUCTION OF THE HRP CIS ELEMENT, AND IDENTIFIES NOVEL CO-REGULATED GENES ARS : Research. ARS Office of International Research Programs. Title: WHOLE-GENOME EXPRESSION PROFILING DEFINES THE HRPL REGULON OF PSEUDOMONAS SYRINGAE PV. TOMATO DC3000, ALLOWS DE NOVO RECONSTRUCTION OF THE HRP CIS ELEMENT, AND IDENTIFIES NOVEL CO-REGULATED GENES Authors. Whole-genome expression profiling defines the hrpl regulon of pseudomonas syringae pv. tomato dc3000, allows de novo reconstruction of the hrp cis element, and identifies novel co-regulated genes. tomato DC3000 is a model bacterial pathogen of tomato and Arabidopsis that deploys a type III secretion system a highly sophisticated secretion system used to introduce specific bacterial proteins called effectors into the plant host cells to disrupt plant cell defenses during pathogenesis. Accordingly, elucidation of the regulatory ...
https://ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=196114
*  High Throughput Gene Expression | Possible Application | UMASS Medical School
High Throughput Gene Expression. Meet the Staff. Advanced Search. Advanced Search. HT Gene Expression. Meet the Staff. HT Gene Expression. Contact Us Co-Director: Kahraman Tanriverdi, PhD Phone: 508-856-6962 kahraman.tanriverdi@umassmed.edu www.umassmed.edu/htcore. HT Gene Expression. Possible Applications High-Throughput Gene Expression Analysis. Ninety-six samples can be analyzed for 96 gene expression assays in under 3 hours. Standard TaqMan SNP assays or your own primers can be used. Users may also choose to submit their genes for DELTAgene assay design service provided by Fluidigm. High-Throughput miRNA Profiling. Ninety-six or more samples can be analyzed for any miRNA assay or entire miRNA assay library 1,801 miRNA Assays in 1-5 business days. Qiagen miScript miRNA assays are provided by core. High-Throughput miRNA Inhibitor Mimic Transfection. Up to 96 miRNA inhibitor or mimic at a time can be transfected to desired cell line and up to 96 gene expression level can be analyzed in a few days with low co...
http://umassmed.edu/htcore/possible_applications/
*  High Throughput Gene Expression | Instrumentation | UMASS Medical School
High Throughput Gene Expression. Instrumentation. Meet the Staff. Instrumentation. Advanced Search. Advanced Search. HT Gene Expression. Meet the Staff. Instrumentation. HT Gene Expression. Instrumentation. Instrumentation. Contact Us Co-Director: Kahraman Tanriverdi, PhD Phone: 508-856-6962 kahraman.tanriverdi@umassmed.edu www.umassmed.edu/htcore. HT Gene Expression. Instrumentation. Instrumentation MagNa Lyser Instrument This instrument simplifies labor-intensive sample preparation. QIAcube Instrument The QIAcube uses QIAGEN spin columns isolation kits, enabling seamless integration of automated, low-throughput sample preparation. JANUS Automated Workstation JANUS Automated Workstation is a liquid handling robot that provides rapid pipetting with superior QC. AB 7900HT Real-Time PCR Instrument In addition to providing fast 96 well, 384 well blocks, the TaqMan Low Density Array TLDA attachment is available for pre-picked gene expression analyses. BioMark Real-Time PCR System The BioMark System provides the a...
http://umassmed.edu/htcore/instrumentation/
*  Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma
... subclasses. What is Broad. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. News & Publications:Scientific Publications Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses. Read More / View Supplemental Materials Somatic mutation in single human neurons tracks developmental and transcriptional history. Read More / View Supplemental Materials Linking RNA biology to lncRNAs. Read More / View Supplemental Materials Ethanol Enhances TGF-β Activity by Recruiting TGF-β Receptors from Intracellular Vesicles/Lipid Rafts/Caveolae to Non-lipid Raft Microdomains. Read More / View Supplemental Materials. Scientific Publications Back to Publications Clas...
https://broadinstitute.org/publications/broad902
*  Browse < ArrayExpress < EMBL-EBI
Browse ArrayExpress EMBL-EBI. Skip to local navigation. Skip to EBI global navigation menu. Skip to expanded EBI global navigation menu includes all sub-sections. Services. Training. Industry. ArrayExpress. Examples: E-MEXP-31, cancer, p53, Geuvadis Advanced. About ArrayExpress. Login. Filter search results By organism: All organisms loading options By experiment type: All assays by molecule loading options. All technologies loading options By array: All arrays loading options. E-GEOD-24551. Exon level expression profiling of colorectal cancer tissue samples. transcription profiling by array. Homo sapiens. - E-GEOD-24550. Exon level expression profiling of colorectal cancer tissue samples validation sample series. transcription profiling by array. Homo sapiens. - E-GEOD-24549. Exon level expression profiling of colorectal cancer tissue samples test sample series. transcription profiling by array. Homo sapiens. - E-GEOD-24030. ChIP-seq, transcription profiling by array. Mus musculus. - E-GEOD-24029. Genome-wid...
http://ebi.ac.uk/arrayexpress/experiments/browse.html?page=1523&pagesize=25
*  Digital Gene-Expression Profiling | GEN Magazine Articles | GEN
Digital Gene-Expression Profiling. Analysis of the RNA-Seq and SCA Publication Landscape. FDA Nominee Driven by "Data, Data, Data". More GEN Exclusives ». Analysis of the RNA-Seq and SCA Publication Landscape. FDA Nominee Driven by "Data, Data, Data". More GEN Exclusives ». Advertorial: OriGene Technologies. Market & Tech An... Jobs. Login/Register. GEN Exclusives More. Analysis of the RNA-Seq and SCA Publication Landscape RNA-Seq and SCA Could Have a... Microarray-based gene-expression profiling technology hardly seems like it has been around long enough for a new approach to come along and challenge its market dominance. Yet, there is a growing sense that digital gene-expression profiling, a fully quantitative approach for gene-expression analysis, will replace microarrays in this application area. Within this emerging market sector for digital RNA counting, the methodologies and technologies are evolving so rapidly that traditional sequencing-based serial analysis of gene-expression approaches are being ch...
http://genengnews.com/gen-articles/digital-gene-expression-profiling/2844/?page=1
*  Use of global gene expression patterns in mechanistic studies of oestrogen action in MCF7 human bre
... ast cancer cells - CentAUR. Use of global gene expression patterns in mechanistic studies of oestrogen action in MCF7 human breast cancer cells. Wordle Link RDF+XML BibTeX RIOXX2 XML RDF+N-Triples JSON Dublin Core Atom Simple Metadata Refer METS HTML Citation with IDs HTML Citation ASCII Citation OpenURL ContextObject EndNote OpenURL ContextObject in Span MODS MPEG-21 DIDL EP3 XML Dublin Core Reference Manager RDF+N3. D 2009 Use of global gene expression patterns in mechanistic studies of oestrogen action in MCF7 human breast cancer cells. Global gene expression profiling has shown that oestrogen action in MCF7 cells involves the coordinated regulation of hundreds of genes across a wide range of functional groupings and that more genes are down regulated than upregulated. Adaptation to long-term oestrogen deprivation, which results in loss of oestrogen-responsive growth, involves alterations to gene patterns not only at early time points 0-4 weeks but continuing through to later times 20-55 weeks, and eve...
http://centaur.reading.ac.uk/9692/
*  BioSpace - A Strategy for Full Interrogation of Prognostic Gene Expression Patterns: Exploring th
... e Biology of Diffuse Large B Cell Lymphoma. Post Jobs. Jobs. A Strategy for Full Interrogation of Prognostic Gene Expression Patterns: Exploring the Biology of Diffuse Large B Cell Lymphoma Published: Thursday, August 04, 2011 Author: Lisa M. LeBlanc Background Gene expression profiling yields quantitative data on gene expression used to create prognostic models that accurately predict patient outcome in diffuse large B cell lymphoma DLBCL. Often, data are analyzed with genes classified by whether they fall above or below the median expression level. We sought to determine whether examining multiple cut-points might be a more powerful technique to investigate the association of gene expression with outcome. Methodology/Principal Findings We explored gene expression profiling data using variable cut-point analysis for 36 genes with reported prognostic value in DLBCL. We plotted two-group survival logrank test statistics against corresponding cut-points of the gene expression levels and smooth estimates of ...
http://biospace.com/PLos_Article.aspx?id=1582
*  DNASTAR - Gene Panel Assembly and Analysis Software | DNASTAR
DNASTAR - Gene Panel Assembly and Analysis Software. DNASTAR. DNASTAR Lasergene. Molecular Biology. Next-Gen Sequencing. Structural Biology. Clinical Research. Molecular Biology. Sanger Sequence Assembly. Sequence Alignment. Structural Biology. Epitope Prediction. Protein Sequence & Structure. Protein Structure Prediction. Structural Alignment. Next-Gen Sequencing. Automated Genome Closure. Cancer Genomics. ChIP-Seq Analysis. De Novo Genome Assembly. De Novo Transcriptome Assembly. Exome Alignment. Gene Panels. Reference Guided Genome Alignment. RNA-Seq Alignment. SNP Validation Control. Variant Analysis. Clinical Research. Cancer Genomics. Gene Panels. SUPPORT. Help. FAQs. Request Support. Supported File Formats. Technical Requirements. Tutorials. NGS Support Materials. #1 in NGS Accuracy. Genome Template Packages. Supported Sequencing Technologies. Genome Assembly. Structure Prediction. Download Free Trial. Request Educational Software. Request Quote. Request Quote. Free Trial. Gene Panel Assembly and Analy...
http://dnastar.com/(F(xcJ6eIahsrvMbyo101SABuX-0UTBU7RcCbYkgxfp9wCWmbsWP_0Ab5AQPhHv5ghQj5SZebDsyBsbZ5LRMeH6cUxWaJ-XPhL0YUuRhsVxkxDHmhL-Oe5wrJf5CEHHebR80CX61c1r-EbJ3ZtpCSvP4GCOfCErUGC81aWh_Ocz3zqUqaw5FuOytOxHc8UOCBHnf-AlqjzaP2mgZRGzUYySPxMZJTU1))/t-sub-solutions-genome-solutions-gene-panels.aspx
*  Multiclass cancer diagnosis using tumor gene expression signatures | Broad Institute of MIT and Harv
Multiclass cancer diagnosis using tumor gene expression signatures. Partnerships Philanthropy Careers Contact Us. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. News & Publications:Scientific Publications Multiclass cancer diagnosis using tumor gene expression signatures. Read More / View Supplemental Materials Modeling Linkage Disequilibrium Increases Accuracy of Polygenic Risk Scores. Read More / View Supplemental Materials. Scientific Publications Back to Publications Multiclass cancer diagnosis using tumor gene expression signatures. To determine whether the diagnosis of multiple common adult malignancies could be achieved purely by molecular classification, we subjected 218 tumor samples, spanning ...
https://broadinstitute.org/publications/broad900
*  PLOS Pathogens: Genome-Wide mRNA Expression Correlates of Viral Control in CD4+ T-Cells from HIV-1-I
... nfected Individuals. Journal Archive. Other Article Types. Article-Level Metrics. Journal Information. View. Sum of PLOS and PubMed Central page views and downloads. Open Access Peer-reviewed Research Article. Genome-Wide mRNA Expression Correlates of Viral Control in CD4+ T-Cells from HIV-1-Infected Individuals. Published: February 26, 2010 DOI: 10.1371/journal.ppat.1000781. Article. 2010 Genome-Wide mRNA Expression Correlates of Viral Control in CD4+ T-Cells from HIV-1-Infected Individuals. doi:10.1371/journal.ppat.1000781 Editor: Michael Emerman, Fred Hutchinson Cancer Research Center, United States of America Received: June 2, 2009; Accepted: January 20, 2010; Published: February 26, 2010 Copyright: © 2010 Rotger et al. Transcriptome analysis in CD4+ T cells from HIV-infected untreated individuals. In total, 260 genes are differentially expressed at adjusted p 0.01 in association with viral load in CD4+ T cells during in vivo HIV-1 infection. Among genes under differential expression during HIV-1 infe...
http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000781
*  A Randomized Phase II Trial Comparing Therapy Based on Tumor Molecular Profiling Versus Conventional
... Therapy in Patients With Refractory Cancer - Full Text View - ClinicalTrials.gov. A Randomized Phase II Trial Comparing Therapy Based on Tumor Molecular Profiling Versus Conventional Therapy in Patients With Refractory Cancer SHIVA This study is currently recruiting participants. If a molecular abnormality is identified for which an approved targeted agent is available, patients are randomized randomized between two arms: Targeted therapy based on the molecular profile Conventional therapy based on investigator's choice. Drug: Targeted therapy based on molecular profiling : Imatinib Procedure: Tumor biopsy Drug: Standard Chemotherapy Drug: Targeted therapy based on molecular profiling : Everolimus Drug: Targeted therapy based on molecular profiling : Vemurafenib Drug: Targeted therapy based on molecular profiling : Sorafenib Drug: Targeted therapy based on molecular profiling : Erlotinib Drug: Targeted therapy based on molecular profiling : Lapatinib + Trastuzumab Drug: Targeted therapy based on molecula...
https://clinicaltrials.gov/ct2/show/NCT01771458
*  Gene expression profiling
... Expression profiling experiments often involve measuring the relative amount of mRNA expressed in two or more experimental conditions. The simplest form of class discovery would be to list all the genes that changed by more than a certain amount between two experimental conditions. Other experiments, such as a Western blot of some of the protein products of differentially expressed genes, make conclusions based on the expression profile more persuasive, since the mRNA levels do not necessarily correlate to the amount of expressed protein. Statistical analysis. Different from the analysis on differentially expressed individual genes, another type of analysis focuses on differential expression or perturbation of pre-defined gene sets and is called gene set analysis. While the statistics may identify which gene products change under experimental conditions, making biological sense of expression profiling rests on knowing which protein each gene product makes and what function this protein performs. Having i...
https://en.wikipedia.org/wiki/Gene_expression_profiling
*  KEGG PATHWAY: Cytokine-cytokine receptor interaction - Canis familiaris (dog)
KEGG PATHWAY: Cytokine-cytokine receptor interaction - Canis familiaris dog. Cytokine-cytokine receptor interaction - Canis familiaris dog. [ Pathway menu Organism menu Pathway entry. Download KGML Show description User data mapping ]. Cytokines are soluble extracellular proteins or glycoproteins that are crucial intercellular regulators and mobilizers of cells engaged in innate as well as adaptive inflammatory host defenses, cell growth, differentiation, cell death, angiogenesis, and development and repair processes aimed at the restoration of homeostasis. Cytokines are released by various cells in the body, usually in response to an activating stimulus, and they induce responses through binding to specific receptors on the cell surface of target cells. Cytokines can be grouped by structure into different families and their receptors can likewise be grouped....
http://genome.jp/kegg-bin/show_pathway?cfa04060
*  Gene signature
... a gene signature is a group of gene s in a cell whose combined expression pattern itadani h mizuarai s kotani h can systems biology understand pathway activation gene expression signatures as surrogate markers for understanding the complexity of pathway activation curr genomics aug pmid is uniquely characteristic of a biological phenotype or medical condition liu j campen a huang s peng sb ye x palakal m dunker ak xia y li s identification of a gene signature in cell cycle pathway for breast cancer prognosis using gene expression profiling data bmc med genomics sep pmid the phenotypes that may theoretically be defined by a gene expression signature range from those that are used to differentiate between different subtypes of a disease those that predict the survival or prognosis of an individual with a disease to those that predict activation of a particular pathway ideally gene signatures can be used to select a group of patients wouters bj löwenberg b erpelinck verschueren ca van putten wl valk pj delw...
https://en.wikipedia.org/wiki/Gene_signature
*  Center for Genomics and Public Health - Gene Expression Profiling in Early-Stage Breast Cancer - Gen
... e Expression Profiling in Early-Stage Breast Cancer Risk-Benefit Analysis Case Study. University of Washington Center for Genomics and Public Health. Home Training. Genetic Awareness. Genomic Applications for Public Health Video Series. Policy & Health Economics Videos. Confounding by Population Stratification. Nutrigenomics. Genetic Epidemiology Methods Video Series. Resources. Tools for Public Health Professionals. Publications. Spotlight Factsheets. Links to Other Resources. In the News. Health Topics. DNA Day. Genetics. Genomics versus Genetics. Genetic Testing: what is it. A Public Health Issue. Evidence-Based Review. EGAPP. Glossary. Links to Genetic Testing Resources. Family History & Genomics. BRFSS. BRFSS Timeline. Compilations. Links and Resources. Risk Benefit for Genetics. Risk Benefit Case Studies and Models. About Us. Director's Page. Future of Public Health Genetics. Technical Assistance. Personnel. Contact Us. Partners. The Gene Expression Profiling in Early-Stage Breast Cancer Risk-Benefi...
http://depts.washington.edu/cgph/riskCaseGeneExprBreastCancer
*  .. Boosting Gene Expression Profiling .. !--h2>
Their research program initially focused on using the high-density Affymetrix Human Exon 1.0 ST array platform for monitoring changes in the expression levels of genes and alternative splicing events of hematopoietic cells, both at the gene and exon levels. Her team was interested in looking at the pattern and gene expression modulations in lineage-specific differentiation of CD34+ cells in vitro, noted Dr. Whole transcriptome and exome analysis on both microarrays and sequencing platforms to identify genes that are significantly up- or downregulated during cell differentiation allowed us to discover novel alternatively spliced transcripts, she further explained. Bowcock, Ph.D., professor, applied deep sequencing techniques in identifying major modifications in the changes in mRNA expression profiles of psoriasis patients. In the case of skin differentiation, miRNA gene expression analysis could reveal dramatic changes in global microRNA expression, reflecting defects in keratinocytes, immune cells, and vascu...
http://genengnews.com/keywordsandtools/print/1/29363/
*  Diagnosis of metastatic neoplasms: Molecular approaches for identification of tissue of origin - Res
... earchGate. Article Diagnosis of metastatic neoplasms: Molecular approaches for identification of tissue of origin. Federico Alberto Monzon. Impact Factor: 2.84. Molecular tests for tissue-of-origin determination in metastatic tumors are available and have the potential to significantly impact patient management. Cost-Effectiveness of Gene-Expression Profiling for Tumor-Site Origin. ABSTRACT: Gene-expression profiling GEP reliably supplements traditional clinicopathological information on the tissue of origin TOO in metastatic or poorly differentiated cancer. The average increase in quality-adjusted life-months was 2.7 months 95% CI 1.5-4.3, and average third-party payer costs per patient increased by $10,360 95% CI $2,982-$19,192. DOI:10.1016/j.jval.2012.09.005 3.28 Impact Factor Source Available from: Torik Ayoubi. "Several studies have already demonstrated that it is possible to use genome-wide gene expression analysis using micro-array technology in the determination of the tissue of origin of CUPs Mon...
http://researchgate.net/publication/41397041_Diagnosis_of_metastatic_neoplasms_Molecular_approaches_for_identification_of_tissue_of_origin
*  Gene Ontology Classifications
... Genes. Human Disease. Expression. Genes. Genes Markers Query. Batch Query. Mouse Genome Browser. Human Disease OMIM Browser. Human Disease. Expression. Gene Expression Data Query. Gene Expression Literature Query. Mouse Developmental Anatomy Browser. GO Browser. Genes Markers Query. Batch Query. Sequences. Mouse Genome Browser. Batch Query. Gene Expression Literature Query. GO Browser. Human Disease OMIM Browser. Mouse Developmental Anatomy Browser. Adult Mouse Anatomy Browser. Batch Query. Mouse Genome Browser. Batch Query. Genes Markers. Sequence Data. Gene Ontology Data. Gene Expression. Mouse Resources & Model Organism Links. Phenotypes Mutants Community Resources. Nucleic Acids Research Journal Database Summary. Tabular View GO Graph GO curators for mouse genes have assigned the following annotations to the gene product of Gm2a. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamine...
http://informatics.jax.org/go/marker/MGI:95762
*  statistics - A mathematician's confusion regarding parametric $t$ tests for gene expression data - B
statistics - A mathematician's confusion regarding parametric $t$ tests for gene expression data - Biology Stack Exchange. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. A mathematician's confusion regarding parametric $t$ tests for gene expression data. I'm a mathematician trying to test some things on gene expression data, and I'm thus skimming over various articles such as Sotiriou et. gene-expression statistics share. asked Oct 25 '12 at 15:10. 5 add a comment. 2 Answers 2 active oldest votes. As a computational biologist I would advise you to look specifically at bioinformatics papers if you are looking into developing new methods, since the analysis in "pure biology" papers can often be lacking and would not give you a good perspective of state-of-the-art analysis metho...
http://biology.stackexchange.com/questions/5010/a-mathematicians-confusion-regarding-parametric-t-tests-for-gene-expression-d?answertab=active
*  .. Axela Takes Over Xceed for Ziplex Automated Gene-Expression Analysis Platform
. Send to printer. GEN News Highlights : Jul 26, 2010. Axela Takes Over Xceed for Ziplex Automated Gene-Expression Analysis Platform. Firms claim combined technologies will help speed introduction of new biomarker diagnostics. Axela has acquired Toronto-based Xceed Molecular to get its hands on the latter s Ziplex gene-expression analysis platform and assays. The combined firm will retain the Axela name but move its headquarters to Xceed s facilities in Toronto. Axela says the combination of both companies technologies will provide clinical researchers with a growing suite of products for multiplexing DNA, RNA, and protein biomarker analysis, and help speed the introduction of new diagnostics. Xceed s Ziplex platform has been developed as an integrated and fully automated hybridization system for multiplex gene-expression signatures. The system hinges on Xceed s Flow-Thru Chip FTC technology for multi-analyte analysis, which is embodied in the single-use TipChip consumables that feature self-regulating fluidi...
http://genengnews.com/keywordsandtools/print/4/19944/
*  The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and diseas
... e. Partnerships Philanthropy Careers Contact Us. Our Approach Areas of Focus History Leadership Who is Broad Partner Institutions Artist-in-Residence Media Center. Press Room News from the Broad Photos for Journalists Spotlight: Ebola Spotlight: CRISPR BroadMinded Blog Video Library For the Scientific Community. Scientific Publications Science Data Software. Scientific Publications Science Data Software. News & Publications:Scientific Publications The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease. Read More / View Supplemental Materials Ethanol Enhances TGF-β Activity by Recruiting TGF-β Receptors from Intracellular Vesicles/Lipid Rafts/Caveolae to Non-lipid Raft Microdomains. Read More / View Supplemental Materials American Diabetes Association and JDRF Research Symposium: Diabetes and the Microbiome. Read More / View Supplemental Materials. Scientific Publications Back to Publications The Connectivity Map: using gene-expression signatures to connect sm...
http://broadinstitute.org/publications/broad3612
*  .. Identifying New Gene-Expression Patterns .. Analyzing Xpress
Identifying New Gene-Expression Patterns. Analyzing XpressWay Human Expression Data with OmniViz Visual Analytics Software. This evaluation is greatly aided by the provision of high-quality gene-expression data and sophisticated software tools for identifying targets with interesting expression patterns. In this article, we describe the combination of Asterand s XpressWay human gene-expression data with BioWisdom s visual analytics software, OmniViz. XpressWay gene-expression data is provided with BioWisdom annotation around gene synonyms, disease, and process information. The inclusion of disease and process information provides context to the gene-expression data, and allows the user to search for interesting targets by virtue of their functional role. Using OmniViz to analyze XpressWay data, it is possible to get an overview of the gene-expression patterns of all ~2,000 genes Figure 1, and then to quickly identify those gene-expression profiles that possess features of interest. For example, if you are int...
http://genengnews.com/keywordsandtools/print/1/12859/
*  BioSpace - Integrated Genomic and Gene Expression Profiling Identifies Two Major Genomic Circuits
... in Urothelial Carcinoma. Post Jobs. Jobs. Career Resources. Career Fairs. Company Profiles. Search Life Sciences Jobs. Integrated Genomic and Gene Expression Profiling Identifies Two Major Genomic Circuits in Urothelial Carcinoma Published: Thursday, June 07, 2012 Author: David Lindgren et al. by David Lindgren, Gottfrid Sjödahl, Martin Lauss, Johan Staaf, Gunilla Chebil, Kristina Lövgren, Sigurdur Gudjonsson, Fredrik Liedberg, Oliver Patschan, Wiking Månsson, Mårten Fernö, Mattias Höglund Similar to other malignancies, urothelial carcinoma UC is characterized by specific recurrent chromosomal aberrations and gene mutations. However, the interconnection between specific genomic alterations, and how patterns of chromosomal alterations adhere to different molecular subgroups of UC, is less clear. We applied tiling resolution array CGH to 146 cases of UC and identified a number of regions harboring recurrent focal genomic amplifications and deletions. Several potential oncogenes were included in the amplifi...
http://biospace.com/PLos_Article.aspx?id=16203
*  This Week in Cancer Research | GenomeWeb
This Week in Cancer Research. GenomeWeb. Skip to main content. RSS Feeds Twitter LinkedIn. Business Policy. Business News Research Funding Policy Legislation Regulatory News Reimbursement. Genetic Research Gene Expression Research Epigenetics Research Proteomics Protein Research Cell Biology Research. Clinical. Molecular Diagnostics Companion Diagnostics Biomarker Discovery Validation Drug Discovery Development Clinical Sequencing Clinical Proteomics. Disease Areas. Cancer Infectious Disease Cardiovascular Disease Neurological Psychological Disease Metabolic Disease Autoimmune Disease Inherited Disease Reproductive Health. Home » This Week in Cancer Research. This Week in Cancer Research Aug 01, 2012. Premium. In Cancer Research this week, a team led by researchers at Laval University in Canada describe the molecular signature found in the lung tissue of smokers. The team carried out genome-wide gene expression profiling on normal lung tissue from 853 lung cancer patients and compared gene expression between ...
https://genomeweb.com/week-cancer-research-20
*  Systems Biology Core Laboratory - New York Medical College
Preparatory techniques: - DNA, RNA and protein extraction, purification, - RNA reverse transcription and fluorescent labeling - DNA and proteins fluorescent labeling - DNA, RNA and protein concentration NanoDrop - DNA, RNA, proteins concentration and QC Bioanalyzer - Western blotting - SpeedVac concentration of proteins, DNAs and RNAs - Controlled PCR amplification B. Hybridization of Agilent microarrays: - Whole Genome Gene Expression microarrays for 30+ species - Exon microarrays human, mouse, rat - miRNA microarrays - CGH Comparative Genomic Hybridization microarrays - CGH+SNP Single Nucleotide Polymorphism microarrays - CNV Copy Number Variant microarrays - ChIP-on-chip Chromatin ImmunoPrecipitation microarrays - DNA methylation CpG islands microarrays C. Hybridization of Illumina beadchips: - Whole Genome Expression BeadChips: human, mouse, rat - Infinium iSelect HD custom genotyping BeadChips for virtually all species - Infinium HumanMethylation450 BeadChip - Infinium HumanCytoSNP-FFPE-12 BeadChips - In...
https://nymc.edu/Research/CoreFacilitiesAndResources/SystemsBiologyCore.html
*  RNAlater® Solution Around the World | FACS Into RNAlater® Solution for Gene Profiling | Thermo Fis
RNAlater® Solution Around the World. FACS Into RNAlater® Solution for Gene Profiling. Thermo Fisher Scientific. Order Support. Custom Products & Projects. Cell Analysis. DNA & RNA Purification. Gene Expression Analysis & Genotyping. PCR. Real-Time PCR. Thermo Scientific. Thermo Scientific. Services. New Products. CaptureSelect™ Products & Services. Diagnostic Products & Services. Thermo Scientific. Air Quality & Water Analysis. Optimized Biopsy Preservation for Clinical Gene Expression and Profiling. Real-Time RT-PCR Directly from Cell Lysates: A Complete Workflow without RNA Purification. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded Tissue. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis. The Ambion RNA later Tissue Collection: RNA Stabilization Solution is an aqueous tissue storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue samples. RNA later Solution eliminates the need to process tissue samples immediately or fr...
https://thermofisher.com/us/en/home/references/ambion-tech-support/rna-isolation/tech-notes/facs-into-rnalater-solution-for-gene-profiling.html
*  Carel A van Baalen - ResearchGate
... For full functionality of ResearchGate it is necessary to enable JavaScript. Here are the instructions how to enable JavaScript in your web browser. Carel A van Baalen. Erasmus MC, Rotterdam, South Holland, Netherlands. Are you Carel A van Baalen. Claim your profile. Publications 36 155.85 Total impact. Article:. DC immunotherapy in HIV-1 infection induces a major blood transcriptome shift. Anna L de Goede. Arno C Andeweg. Henk-Jan van den Ham. Maarten A Bijl. Fatiha Zaaraoui-Boutahar. Wilfred F J van IJcken. Sofie Wilgenhof. Joeri L Aerts. Rob A Gruters. Albert D M E Osterhaus. Sabine D Allard Carel A van Baalen. Brenda De Keersmaecker. Marchina E van der Ende. Kris Thielemans. Patrick Lacor. ABSTRACT: This study aimed to evaluate the effect of dendritic cell DC vaccination against HIV-1 on host gene expression profiles. Longitudinal PBMC samples were collected from participants of the DC-TRN trial for immunotherapy against HIV. Microarray-assisted gene expression profiling was performed to evaluate the...
http://researchgate.net/researcher/38566550_Carel_A_van_Baalen
*  Anti -DAB2, CT (DOC-2, Differentially-expressed Protein 2, Disabled Homolog 2, DOC2) (Biotin) - Unit
Anti -DAB2, CT DOC-2, Differentially-expressed Protein 2, Disabled Homolog 2, DOC2 Biotin - United States Biological. Login. Email. Password. Forgot your password. New User. Remember me. Home. Distributors. Technical. About. Register. Antibodies. Biochemicals. Culture Media. Custom Services. Growth Factors. Bioassay Kits. Lectins. Molecular Biology. Serum, Tissues. Protocols. Newsletter. About Us. Contact. . You are here: Home. Antibodies. Antibodies-Tumor Suppressors. Anti -DAB2, CT DOC-2, Differentially-expressed Protein 2, Disabled Homolog 2, DOC2 Biotin Anti -DAB2, CT DOC-2, Differentially-expressed Protein 2, Disabled Homolog 2, DOC2 Biotin. Pricing For pricing information, USA customers sign in. Outside USA. Please contact your distributor for pricing. Specifications Clone. Host. Grade Applications Polyclonal Rabbit Affinity Purified E B. DAB2 is the component of the CSF-1 signal transduction pathway. Catalog # D0900-19C-Biotin. Applications Suitable for use in ELISA and Western Blot. Other applications...
http://usbio.net/item/D0900-19C-Biotin

Cellular microarray: A cellular microarray is a laboratory tool that allows for the multiplex interrogation of living cells on the surface of a solid support. The support, sometimes called a "chip", is spotted with varying materials, such as antibodies, proteins, or lipids, which can interact with the cells, leading to their capture on specific spots.Allele-specific oligonucleotide: An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay.Gene signature: A gene signature is a group of genes in a cell whose combined expression patternItadani H, Mizuarai S, Kotani H. Can systems biology understand pathway activation?DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Abscription: During normal transcription, RNA polymerase transcribes a number of short nonproductive oligonucleotides, and this process is called abortive transcription. The trapped RNAPs have been named abscriptases and the synthesis of specific length oligonucleotides called abscription.Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Coles PhillipsThermal cyclerProtein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Generalizability theory: Generalizability theory, or G Theory, is a statistical framework for conceptualizing, investigating, and designing reliable observations. It is used to determine the reliability (i.Community Fingerprinting: Community fingerprinting refers to a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community. Rather than directly identifying or counting individual cells in an environmental sample, these techniques show how many variants of a gene are present.Copy number analysis: Copy number analysis usually refers to the process of analyzing data produced by a test for DNA copy number variation in patient's sample. Such analysis helps detect chromosomal copy number variation that may cause or may increase risks of various critical disorders.Mature messenger RNA: Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, it consists exclusively of exons, with all introns removed.Assay sensitivity: Assay sensitivity is a property of a clinical trial defined as the ability of a trial to distinguish an effective treatment from a less effective or ineffective intervention. Without assay sensitivity, a trial is not internally valid and is not capable of comparing the efficacy of two interventions.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Clonal Selection Algorithm: In artificial immune systems, Clonal selection algorithms are a class of algorithms inspired by the clonal selection theory of acquired immunity that explains how B and T lymphocytes improve their response to antigens over time called affinity maturation. These algorithms focus on the Darwinian attributes of the theory where selection is inspired by the affinity of antigen-antibody interactions, reproduction is inspired by cell division, and variation is inspired by somatic hypermutation.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Antisense therapy: Antisense therapy is a form of treatment for genetic disorders or infections. When the genetic sequence of a particular gene is known to be causative of a particular disease, it is possible to synthesize a strand of nucleic acid (DNA, RNA or a chemical analogue) that will bind to the messenger RNA (mRNA) produced by that gene and inactivate it, effectively turning that gene "off".YjdF RNA motifEukaryotic transcription: Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Chromosome regionsBorosilicate glass: Borosilicate glass is a type of glass with silica and boron trioxide as the main glass-forming constituents. Borosilicate glasses are known for having very low coefficients of thermal expansion (~3 × 10−6 /°C at 20 °C), making them resistant to thermal shock, more so than any other common glass.Fixative (drawing): In drawing, a fixative is a liquid, similar to varnish, which is usually sprayed over a finished piece of artwork, usually a dry media artwork, to better preserve it and prevent smudging.CpG OligodeoxynucleotideAlternative splicing: Alternative splicing is a regulated process during gene expression that results in a single gene coding for multiple proteins. In this process, particular exons of a gene may be included within or excluded from the final, processed messenger RNA (mRNA) produced from that gene.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.Genetic variation: right|thumbWGAViewer: WGAViewer is a bioinformatics software tool which is designed to visualize, annotate, and help interpret the results generated from a genome wide association study (GWAS). Alongside the P values of association, WGAViewer allows a researcher to visualize and consider other supporting evidence, such as the genomic context of the SNP, linkage disequilibrium (LD) with ungenotyped SNPs, gene expression database, and the evidence from other GWAS projects, when determining the potential importance of an individual SNP.Mac OS X Server 1.0Amplified Ribosomal DNA Restriction Analysis: Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic amplification using primers directed at the conserved regions at the ends of the 16s gene, followed by digestion using tetracutter Restriction enzymes.ParaHox: The ParaHox gene cluster is an array of homeobox genes (involved in morphogenesis, the regulation of patterns of anatomical development) from the Gsx, Xlox (Pdx) and Cdx gene families.PSI Protein Classifier: PSI Protein Classifier is a program generalizing the results of both successive and independent iterations of the PSI-BLAST program. PSI Protein Classifier determines belonging of the found by PSI-BLAST proteins to the known families.Ontario Genomics Institute: The Ontario Genomics Institute (OGI) is a not-for-profit organization that manages cutting-edge genomics research projects and platforms.The Ontario Genomics Institute OGI also helps scientists find paths to the marketplace for their discoveries and the products to which they lead, and it works through diverse outreach and educational activities to raise awareness and facilitate informed public dialogue about genomics and its social impacts.Polymerase-endonuclease amplification reaction: Polymerase-endonuclease amplification reaction (PEAR) is a DNA amplification technology for the amplification of oligonucleotides. A target oligonucleotide and a tandem repeated antisense probe are subjected to repeated cycles of denaturing, annealing, elongation and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI) cleavage releases monomeric duplex oligonucleotides.Sequence clustering: In bioinformatics, sequence clustering algorithms attempt to group biological sequences that are somehow related. The sequences can be either of genomic, "transcriptomic" (ESTs) or protein origin.Pituitary-specific positive transcription factor 1: POU domain, class 1, transcription factor 1 (Pit1, growth hormone factor 1), also known as POU1F1, is a transcription factor for growth hormone.Inverse probability weighting: Inverse probability weighting is a statistical technique for calculating statistics standardized to a population different from that in which the data was collected. Study designs with a disparate sampling population and population of target inference (target population) are common in application.Genetic imbalance: Genetic imbalance is to describe situation when the genome of a cell or organism has more copies of some genes than other genes due to chromosomal rearrangements or aneuploidy.CS-BLASTInfinite alleles model: The infinite alleles model is a mathematical model for calculating genetic mutations. The Japanese geneticist Motoo Kimura and American geneticist James F.DNA-binding proteinCertified reference materials: Certified Reference Materials (CRMs) are ‘controls’ or standards used to check the quality and metrological traceability of products, to validate analytical measurement methods, or for the calibration of instruments.Deletion (genetics)Phenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.GC box: In molecular biology, a GC box is a distinct pattern of nucleotides found in the promoter region of some eukaryotic genes upstream of the TATA box and approximately 110 bases upstream from the transcription initiation site. It has a consensus sequence GGGCGG which is position dependent and orientation independent.List of sequenced eukaryotic genomesExtracellular: In cell biology, molecular biology and related fields, the word extracellular (or sometimes extracellular space) means "outside the cell". This space is usually taken to be outside the plasma membranes, and occupied by fluid.Analytical quality control: Analytical quality control, commonly shortened to AQC refers to all those processes and procedures designed to ensure that the results of laboratory analysis are consistent, comparable, accurate and within specified limits of precision.analytical quality control (AQC) program to ensure the highest level of confidence in reported data Constituents submitted to the analytical laboratory must be accurately described to avoid faulty interpretations, approximations, or incorrect results.List of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.Gene duplication: Gene duplication (or chromosomal duplication or gene amplification) is a major mechanism through which new genetic material is generated during molecular evolution. It can be defined as any duplication of a region of DNA that contains a gene.Nucleic acid structure: Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Transfer-messenger RNA: Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1.Gene polymorphismTriparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.Interval boundary element method: Interval boundary element method is classical boundary element method with the interval parameters.
Lattice protein: Lattice proteins are highly simplified computer models of proteins which are used to investigate protein folding.Open reading frame: In molecular genetics, an open reading frame (ORF) is the part of a reading frame that has the potential to code for a protein or peptide. An ORF is a continuous stretch of codons that do not contain a stop codon (usually UAA, UAG or UGA).

(1/28523) A novel method for determining linkage between DNA sequences: hybridization to paired probe arrays.

Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two different probe sequences. Each of the two probes can hybridize independently to a different target sequence, but if the two target sequences are physically linked there is a cooperative increase in hybridization yield. The ability to create and control non-linear effects raises a host of possibilities for applications of oligonucleotide array hybridization. The method has been used to assign linkage in 50:50 mixtures of DNA containing single nucleotide polymorphisms (SNPs) separated by 17, 693, 1350 and 2038 bp and to reconstruct haplotypes. Other potential uses include increasing the specificity of hybridization in mutation detection and gene expression monitoring applications, determining SNP haplotypes, characterizing repetitive sequences, such as short tandem repeats, and aiding contig assembly in sequen-cing by hybridization.  (+info)

(2/28523) Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents.

The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.  (+info)

(3/28523) Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes.

Comparing patterns of gene expression in cell lines and tissues has important applications in a variety of biological systems. In this study we have examined whether the emerging technology of cDNA microarrays will allow a high throughput analysis of expression of cDNA clones generated by suppression subtractive hybridization (SSH). A set of cDNA clones including 332 SSH inserts amplified by PCR was arrayed using robotic printing. The cDNA arrays were hybridized with fluorescent labeled probes prepared from RNA from ER-positive (MCF7 and T47D) and ER-negative (MDA-MB-231 and HBL-100) breast cancer cell lines. Ten clones were identified that were over-expressed by at least a factor of five in the ER-positive cell lines. Northern blot analysis confirmed over-expression of these 10 cDNAs. Sequence analysis identified four of these clones as cytokeratin 19, GATA-3, CD24 and glutathione-S-transferase mu-3. Of the remaining six cDNA clones, four clones matched EST sequences from two different genes and two clones were novel sequences. Flow cytometry and immunofluorescence confirmed that CD24 protein was over-expressed in the ER-positive cell lines. We conclude that SSH and microarray technology can be successfully applied to identify differentially expressed genes. This approach allowed the identification of differentially expressed genes without the need to obtain previously cloned cDNAs.  (+info)

(4/28523) Identification of the genes responsive to etoposide-induced apoptosis: application of DNA chip technology.

DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of detecting 6591 unique human mRNA species. 12 (Nine induced and three repressed) of the etoposide-responsive genes were further studied by Northern analysis and an agreement rate of 92%, was reached. Among the 12 genes studied, two (WAF1/p21 and PCNA) are known p53 regulatory genes, two (glutathione peroxidase and S100A2 calcium-binding protein) appear to be the novel p53 target genes and the others appear to be p53-independent. Based upon these findings, the signalling pathways that possibly mediate etoposide-induced apoptosis are proposed.  (+info)

(5/28523) Development of an oligonucleotide-specific capture plate hybridization assay for detection of Haemophilus parasuis.

An oligonucleotide-specific capture plate hybridization assay has been developed to rapidly, specifically, and sensitively detect Haemophilus parasuis from nasal swabs. Several in vitro studies have been performed to determine the sensitivity and specificity of the test, and in vivo studies have validated this technique in pigs. Results suggest that the assay detects <100 colony-forming units/ml in a pure culture and gives a positive result when H. parasuis is present in a ratio of 1:10(3)-10(4) in a mixed culture, and the probe does not hybridize with other related species found in the upper respiratory tract. This assay is more sensitive than culture for detection of the microorganism from nasal swabs and lesions.  (+info)

(6/28523) Versatile derivatisation of solid support media for covalent bonding on DNA-microchips.

A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces. Bonding was found to be stable even after some 30 cycles of stripping. Due to a dendrimeric structure of the linker molecule, the loading can be modified in a controlled manner and increased beyond the capacity of glass without negative effects on hybridisation efficiency. Also, the chemistry warrants the modulation of other surface properties such as charge or hydrophobicity. Preferentially, attachment of nucleic acids takes place only via the terminal amino-group of amino-modified oligonucleotides or the terminal hydroxyl-group of unmodified molecules so that the entire molecule is accessible to probe hybridisation. This derivatisation represents a support chemistry versatile enough to serve nearly all current forms of DNA-arrays or microchips.  (+info)

(7/28523) Timely toxicology.

The ToxChip, a DNA microarray chip, allows the monitoring of the expression levels of thousands of different genes at a time, thereby condensing months of painstaking laboratory tasks into a day's work. For toxicology researchers in particular, this tool is important because it promises a more effective way to identify environmental hazards and their effects on DNA. The ToxChip, developed by NIEHS scientists J. Carl Barrett, Cynthia Afshari, and Emile F. Nuwaysir, could transform the way toxicologists approach environmental problems.  (+info)

(8/28523) DNA microarray technology: the anticipated impact on the study of human disease.

One can imagine that, one day, there will be a general requirement that relevant array data be deposited, at the time of publication of manuscripts in which they are described, into a single site made available for the storage and analysis of array data (modeled after the GenBank submission requirements for DNA sequence information). With this system in place, one can anticipate a time when data from thousands of gene expression experiments will be available for meta-analysis, which has the potential to balance out artifacts from many individual studies, thus leading to more robust results and subtle conclusions. This will require that data adhere to some type of uniform structure and format that would ideally be independent of the particular expression technology used to generate it. The pros and cons of various publication modalities for these large electronic data sets have been discussed elsewhere [12], but, practical difficulties aside, general depositing must occur for this technology to reach the broadest range of investigators. Finally, as mentioned at the beginning of this review, it is unfortunate that this important research tool remains largely restricted to a few laboratories that have developed expertise in this area and to a growing number of commercial interests. Ultimately the real value of microarray technology will only be realized when this approach is generally available. It is hoped that issues including platforms, instrumentation, clone availability, and patents [20] will be resolved shortly, making this technology accessible to the broadest range of scientists at the earliest possible moment.  (+info)


What is the nutritional analysis for 15% drained ground beef?


What is the nutritional analysis for 15% drained ground beef?
I've had this sort of problem before (though rarely); that is, finding basic info on something as ubiquitous as drained ground beef. But go ahead and google for nutritional analysis "drained ground beef". 9 entries! It's insane! 
So does anyone know what it is?
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The USDA does wonderful things and this is no exception:
http://www.fns.usda.gov/fdd/facts/schfacts/intro-schoolfacts.htm


How can i get blood drawn for a nutritional analysis without a doctor's order?


I am currently seeing a nutritionist , who wants me to get a nutritional analysis done.  I have the kit with vials and instructions to send in the test.  I am having a difficult time finding a nurse and/or phlebotionist to draw the blood without a doctors order.  In the state of Illinois a nutritionist is not considered a doctor.  Is there an online doctor that can order my blood drawn?  Any information will be greatly appreciated.  Thank You!!!
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You can't by law. If you come across a facility that actually draws blood with a needle without being properly certified or under a medical physician then you better run the other direction. They do, do blood stains but those are generally done by finger pricks and sometimes the patient or client does that themselves. Naturopathic physicians or counselors or Nutritionists can lead you in that direction. 

For starters don't over analyze what you are missing. Try detoxing by eliminating all inappropriate foods. Then start out right by eating fruits and veggies and then low fat proteins such as fat and chicken. It's common sense. Then follow with a multivitamin. People can try every technique possible to short cut for a healthy body, but it will always come down to proper eating.


How long does it take to get results from a semen analysis?


My husband had to do a semen analysis and I was wondering if anyone knows how long it takes to get the results back.  He did it two days ago.
----------

He should be able to call and get the results now.  They have to do the analysis almost immediately while the sperm are still alive and "fresh".  Some places only call if the results are abnormal, some wait until the two of you come in together on your next visit.  But they should have the results by now and he should be able to call and get them today.


What should be the sequence of exercise?


I watched some videos on youtube for exercise at home. Every trainer in those videos recommended to do them in the sequence shown. Well, I do a mix of excesaise shown in different videos so I want to know in which sequence I should do those exercises to get the best effect. Is there any sequence like first I should it for shoulders then biceps then triceps then chest, abs, legs and so on ?
----------

In general you can have a upper body exercise on one day and lower body exercise on another day. This gives sufficient relaxation to your muscle and also do not pain very hard. I usually follow exercise from upper to lower like shoulder, biceps, triceps, fore arms, etc.


What is the nutritional analysis for 15% drained ground beef?


What is the nutritional analysis for 15% drained ground beef?
I've had this sort of problem before (though rarely); that is, finding basic info on something as ubiquitous as drained ground beef. But go ahead and google for nutritional analysis "drained ground beef". 9 entries! It's insane! 
So does anyone know what it is?
----------

What for do u want to load urself with statistical info?

Eat only when u r hungry.

Eat nourishing food with plenty of fresh fruits and vegetables being part of it. Chew each morsel at least 32 times. This will activate signal to the brain as soon as u have had enough. Besides this change in eating style, take regular light exercises and brisk walks every day. U will be able to gain/shed all extra weight gracefully and in a reasonable time span. No food or drink in between the food and not more than three meals a day, unless u r diabetic.


What is the nutritional analysis for 15% drained ground beef?


What is the nutritional analysis for 15% drained ground beef?
I've had this sort of problem before (though rarely); that is, finding basic info on something as ubiquitous as drained ground beef. But go ahead and google for nutritional analysis "drained ground beef". 9 entries! It's insane! 
So does anyone know what it is?
----------

Eat only when u r hungry.

Eat nourishing food with plenty of fresh fruits and vegetables being part of it. Chew each morsel at least 32 times. This will activate signal to the brain as soon as u have had enough. Besides this change in eating style, take regular light exercises and brisk walks every day. U will be able to gain/shed all extra weight gracefully and in a reasonable time span. No food or drink in between the food and not more than three meals a day, unless u r diabetic.


How will i sequence these exercises in my strength building routine?


Currently i do exercises such as push ups, dips, sit ups, bicep curls and sometimes chin ups. I can do 3 sets of 20 push ups, 2 sets of 15 dips, 3 sets of 8 bicep curls, 1 set of 35 sit ups and barely 2 reps of chin ups. can anyone tell me how will i sequence these exercises as in what after what and how long break will i take between them? a proper comprehensive answer is what i want for the best answer.
----------

If these are the only exercises that you are doing, then i suggest you aim for more repetitions for your push ups and sit ups. That said, it seems like you're at the beginning stage of your strength training program, so what you are doing is alright, but not fantastic. 

The sequence that you should do the exercises that you are currently doing should all affect the same muscle groups (i.e. chest + triceps, back + biceps, and abs can be done separately after your main workouts). 

Chest + Triceps: 3 sets up push ups at a 1 minute rest interval. When you're done your push ups do your 2 sets of dips, also at a 1 minute rest interval. 

The same idea for your back + biceps: Chin ups and bicep curls. 

Chin ups/Pull ups are fantastic workouts, and they are perfect for training upper body strength, so do not skip out on doing them. If you're doing 2 normal reps now, I suggest you keep working on your chin ups/pull ups. In order to increase the amount of chin ups you can do, try doing negatives. 

Here's how to do a negative chin up: http://www.youtube.com/watch?v=Opr72q6NKTc


What is the best sequence for the following?


* Dinner
* Jogging
* Gym
* Swimming
* Steam Bath

I want to do all these activities in just one night -- Every night if possible.
My available time is from 7 pm onwards, do you think it's possible?

If you can arrange the sequence with precise explanation that would be great! :)

thanks everyone in advance!
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Gina is 100% correct!