Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Continental Population Groups: Groups of individuals whose putative ancestry is from native continental populations based on similarities in physical appearance.Nucleic Acid Denaturation: Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.School Health Services: Preventive health services provided for students. It excludes college or university students.Hospitals, Federal: Hospitals controlled by agencies and departments of the U.S. federal government.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Peptide Nucleic Acids: DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Methods: A series of steps taken in order to conduct research.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Parenteral Nutrition, Total: The delivery of nutrients for assimilation and utilization by a patient whose sole source of nutrients is via solutions administered intravenously, subcutaneously, or by some other non-alimentary route. The basic components of TPN solutions are protein hydrolysates or free amino acid mixtures, monosaccharides, and electrolytes. Components are selected for their ability to reverse catabolism, promote anabolism, and build structural proteins.TritiumTerahertz Imaging: The visualization of internal structure using TERAHERTZ RADIATION technologies.Triprolidine: Histamine H1 antagonist used in allergic rhinitis; ASTHMA; and URTICARIA. It is a component of COUGH and COLD medicines. It may cause drowsiness.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Scavenger Receptors, Class A: A family of scavenger receptors that mediate the influx of LIPIDS into MACROPHAGES and are involved in FOAM CELL formation.Pituitary Gland, Anterior: The anterior glandular lobe of the pituitary gland, also known as the adenohypophysis. It secretes the ADENOHYPOPHYSEAL HORMONES that regulate vital functions such as GROWTH; METABOLISM; and REPRODUCTION.RNA-Directed DNA Polymerase: An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Eicosanoids: A class of compounds named after and generally derived from C20 fatty acids (EICOSANOIC ACIDS) that includes PROSTAGLANDINS; LEUKOTRIENES; THROMBOXANES, and HYDROXYEICOSATETRAENOIC ACIDS. They have hormone-like effects mediated by specialized receptors (RECEPTORS, EICOSANOID).Hybridization, Genetic: The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.Micropore Filters: A membrane or barrier with micrometer sized pores used for separation purification processes.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Cell Line: Established cell cultures that have the potential to propagate indefinitely.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Nucleic Acid Probes: Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Plant Infertility: The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998)Platelet-Rich Plasma: A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Yttrium Radioisotopes: Unstable isotopes of yttrium that decay or disintegrate emitting radiation. Y atoms with atomic weights 82-88 and 90-96 are radioactive yttrium isotopes.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Kinetics: The rate dynamics in chemical or physical systems.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Goiter: Enlargement of the THYROID GLAND that may increase from about 20 grams to hundreds of grams in human adults. Goiter is observed in individuals with normal thyroid function (euthyroidism), thyroid deficiency (HYPOTHYROIDISM), or hormone overproduction (HYPERTHYROIDISM). Goiter may be congenital or acquired, sporadic or endemic (GOITER, ENDEMIC).RNA Probes: RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.

*  Draft:Hybridization assay
Hybridization assays involve labelled nucleic acid ... i.e. Sandwich hybridization assay Competitive hybridization assay Hybridization-ligation assay Dual ligation hybridization assay Nuclease hybridization assay...
https://en.wikipedia.org/wiki/Draft:Hybridization_assay
*  Hybridisation
... or hybridization may refer to hybridisation biology ... a hybrid nucleic acid hybridization the process of ... strands of nucleic acids rna dna or ... hybridisation hybridisation hybridisation or hybridization may refer to hybridisation biology the process of combining different varieties of organisms to create a hybrid nucleic acid hybridization the process of joining two complementary strands of nucleic acids rna dna or oligonucleotides in chemistry the mixing of atomic orbitals to form new orbitals suitable for bonding orbital hybridisation in evolutionary algorithm s the merging two or more optimization techniques into a single algorithm memetic algorithm a common template for hybridisation in linguistics the process of one variety blending with another variety the alteration of a vehicle into a hybrid electric vehicle in globalization theory the ongoing blending of cultures hybridization in political election campaign communication the combining of campaign techniqu...
https://en.wikipedia.org/wiki/Hybridisation
*  Hybridization probe
Hybridization_probe Hybridization_probe. In molecular biology , X '''hybridization probe''' is a fragment of DNA or RNA of ... to single-stranded nucleic acid DNA or RNA whose base sequence ... Hybridization probe Hybridization probe. In molecular biology, X 'hybridization probe' is a fragment of DNA or RNA of variable length usually 100-1000 bases long which is radioactively labeled. The probe thereby hybridizes to single-stranded nucleic acid DNA or RNA whose base sequence allows probe-target base pair ing due to complementarity between the probe and target. The labeled probe is first denatured by heating or under alkaline conditions such as exposure to sodium hydroxide into single stranded DNA ssDNA and then hybridized to the target ssDNA Southern blot ting or RNA Northern blot ting immobilized on a membrane or in situ. To detect hybridization of the probe to its target sequence, the probe is tagged or "labeled" with a molecular marker of either radioactive or more recently fluorescent molecules; commonl...
https://en.wikipedia.org/wiki/Hybridization_probe
*  Helicase
along a nucleic acid phosphodiester backbone , separating ... two annealed nucleic acid strands i.e., DNA , RNA , or RNA- ... to destabilize the nucleic acids, unwinding the double-helix at a ... They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands i.e., DNA, RNA, or RNA-DNA hybrid using energy derived from ATP hydrolysis. Function Activation barrier in helicase activity. RecQ family mutations. Activation barrier in helicase activity. In active helicases, 'B' < k B T, where the system lacks a significant barrier, as the helicase is able to destabilize the nucleic acids, unwinding the double-helix at a constant rate, regardless of the nucleic acid sequence. 'Superfamily 1 SF1 ': This superfamily can be further subdivided into SF1A and SF1B helicases. In this group helicases can have either 3’-5’ SF1A subfamily or 5’-3’ SF1B subfamily translocation polarity. This group is mainly composed of DEAD-box RNA helicases. 'Superfam...
https://en.wikipedia.org/wiki/Helicase
*  R-loop
is a three-stranded nucleic acid structure, composed of a DNA: RNA ... be created by the hybridization of mature mRNA with double-stranded DNA ... and resolution. “Hybridization of RNA to double-stranded DNA:...
https://en.wikipedia.org/wiki/R-loop
*  Nucleic acid double helix
... ... molecules of nucleic acid s such as DNA. 2 In B-DNA, the ... groove. 4 History Nucleic acid hybridization Base pair geometry ... Nucleic acid double helix Nucleic acid double helix. "Double helix". Double helix??. 1 refers to the structure formed by double-stranded molecules of nucleic acid s such as DNA. 2 In B-DNA, the most common double helical structure, the double helix is right-handed with about 10 10.5 base pairs per turn. The double helix structure of DNA contains a 'major groove' and 'minor groove'. In B-DNA the major groove is wider than the minor groove. 4 History Nucleic acid hybridization Base pair geometry Helix geometries Grooves. Non-double helical forms. Bending Persistence length/axial stiffness. The double helix makes one complete turn about its axis every 10.4-10.5 base pairs in solution. The narrowness of the minor groove means that the edges of the bases are more accessible in the major groove. Single-stranded nucleic acids do not adopt a helical formation, and are described...
https://en.wikipedia.org/wiki/Nucleic_acid_double_helix
*  Draft:Dual ligation assay
dual ligation hybridization assay is a ligand binding assay ... a type of hybridization assay used to quantify nucleic acids ... are quantified with hybridization assays in biological matrices such as...
https://en.wikipedia.org/wiki/Draft:Dual_ligation_assay
*  Patent US5110932 - Polysubstituted aryl acridinium esters - Google Patente
as immunoassays or nucleic acid hybridization assays. More ... as immunoassays or nucleic acid hybridization assays. More ... A luminescent polysubstituted aryl acridinium ester selected from the group having the following structure: ##STR5## where R 1 is an alkyl, alkenyl, alkynyl, or aryl group; R 2, R 3, R 5, or R 7 are a hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro, or halide group; R 4 or R 8 are an, alkoxyl, group; R 6 represents hydrogen or the following substituent: R 6 =-R 9 -R 10. 3 A luminescent polysubstituted aryl acridinium ester selected from the group having the following structure: ##STR8## where R 1 is an alkyl, alkenyl, alkynyl, or aryl group; R 2, R 3, R 5, or R 7 are a hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro, or halide group; R 4 or R 8 are an alkyl group; R 6 represents hydrogen; X is CH 3 SO 4 -, - OSO 2 F, a halide, - OSO 2 CF 3, - OSO 2 C 4 F 9, or ##STR9## and the R 5, R 6 and R 7 substituent positions on the phenoxy ring are interchangeable. Other luminescent...
http://google.de/patents/US5110932
*  Sol Spiegelman
the technique of nucleic acid hybridization, which helped to lay ... in St. Called nucleic acid hybridization originally developed ... Sol Spiegelman Sol Spiegelman. name = Sol Spiegelman image = SolSpiegelman.jpg image size = 130px. caption = Sol Spiegelman birth date =. birth place = Brooklyn, New York, U.S. nationality = American. death date = 'Sol Spiegelman' December 14, 1914 – January 20, 1983 was an American molecular biologist. He developed the technique of nucleic acid hybridization, which helped to lay the groundwork for advances in recombinant DNA technology. Spiegelman was born and educated in New York City, and earned a bachelor's degree in mathematics from the City College of New York in 1939. He began his graduate studies at Columbia University in 1940, looking into cellular physiology. He completed his graduate studies at Washington University in St. Called nucleic acid hybridization originally developed by Rich and Davies in 1956,. 1 it was the combination of viral DNA and viral RNA which he...
https://en.wikipedia.org/wiki/Sol_Spiegelman
*  Heteroduplex
molecule of nucleic acid originated through the genetic ... strand formed in hybridization processes usually for biochemistry ... natural analogs of nucleic acids are used to bind with nucleic acids these heteroduplexes result from ... heteroduplex heteroduplex a heteroduplex is a double stranded duplex molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources such as from different homologous chromosomes or even from different organisms one such example is the heteroduplex dna strand formed in hybridization processes usually for biochemistry based phylogenetic al analyses another example is the heteroduplexes formed when non natural analogs of nucleic acids are used to bind with nucleic acids these heteroduplexes result from performing antisense techniques using single stranded peptide nucleic acid o methyl phosphorothioate or morpholino oligos to bind with rna in meiosis the process of crossing over occurs between non...
https://en.wikipedia.org/wiki/Heteroduplex
*  Fluorescence in situ hybridization
... thumb|Multiplex RNA visualization in cells using ViewRNA FISH Assays Image:Bcrablmet.jpg 'Fluorescence in situ hybridization ' 'FISH' is a cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity. 2 FISH can also be used to detect and localize specific RNA targets mRNA, lncRNA and miRNA in cells, circulating tumor cells, and tissue samples. Probes – RNA and DNA Preparation and hybridization process – RNA. Preparation and hybridization process – DNA. Probes – RNA and DNA. thumb|ViewRNA detection of miR-133 green and myogenin mRNA red in C2C12 differentiating cells RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA,. For miRNA detection, the probes use proprietary chemistry for specific detection of miRNA and cover the entire miRNA sequence. Preparation and hybridization process – RNA. Preparation and hybridization process – DNA. The probe must be large enough to hybridize s...
https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization
*  Chlamydia trachomatis nucleic acid hybridization test
Health, Conditions Treatments. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Cervical cells/tissue: Written consent may be required for a Pap smear or colposcopy with cervical biopsy. Tell the healthcare worker if you have a medical condition or are using a medication or supplement that causes excessive bleeding. This will make it easier for the healthcare worker to see your cervix during the procedure, and may make the procedure more comfortable for you. Urethral cells/discharge: Before collection of urethral cells and/or urethral discharge for this test, you should not urinate during the hour prior to the test. An endocervical or urethral sample may be collected for this test. Cervical cells/tissue: For both a Pap smear and colposcopy with cervical biopsy, you will be asked to lie on your back with your legs spread and feet placed in stirrups. Urethral cells/discharge: A urethral culture procedure is used to collect cells samples and/or ureth...
http://allinahealth.org/CCS/doc/Consumer_Lab/49/150346.htm
*  PCR primers and hybridization probes
... linda j carter ljc at cas org mon sep est previous message flexibility sleep next message pcr primers and hybridization probes messages sorted by for those of you interested in pcr primers and hybridization probes i would really appreciate your opinions about their inclusion in biosequence databases some of my specific concerns are as follows do you feel primers and probes tend to clutter up a nucleic acid database and cause unnecessary retrievals when searching for sequences of much longer length because primers and probes are usually designed from known sequence information often already in the database is it still justifiable to include them as additional entries what applications of primers and probes should justify their inclusion in a biosequence database e g clinical diagnosis taxonomy evolution gene mapping or methods if you were to search for primers and probes in a biosequence database what would be most efficient for you would some type of descriptive information be helpful e g their applicati...
http://bio.net/bionet/mm/bioforum/1993-September/005951.html
*  Sequencing by hybridization
... 'Sequencing by hybridization' is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence. The binding of one strand of DNA to its complementary strand in the DNA double-helix aka hybridization is sensitive to even single-base mismatches when the hybrid region is short or if specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notable via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations. The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation as well as pore-based methods. However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, an...
https://en.wikipedia.org/wiki/Sequencing_by_hybridization
*  My dot blot hybridization doesn't work! - Molecular Biology
my dot blot hybridization doesn t work molecular biology biojob bioblog pubalert biotool bioproduct bioforum protocol home forum index home live discussion top forum archives molecular biology my dot blot hybridization doesn t work sep pages previous bacterial growth in these solutions would be bad because bacteria produce proteases which will break down your antibodies milk is a pretty good medium for any number of microorganisms phage quote phage oct am bacterial growth in these solutions would be bad because bacteria produce proteases which will break down your antibodies milk is a pretty good medium for any number of microorganisms ooo now i know about that thanks for the information on the other hand i would like to do direct spotting on membrane using non extracted sample which is suspended sample the protocol given is spot or microlitre of the suspended sample onto the membrane let it completely air dried and then incubate in blotting buffer x ssc m formaldehyde for minute in degree celcius after minut...
http://protocol-online.org/biology-forums/posts/30450more2.html
*  PCR primers and hybridization probes
... linda j carter ljc at cas org mon sep est previous message sequence data submissions next message hmmmmmm messages sorted by for those of you interested in pcr primers and hybridization probes i would really appreciate your opinions about their inclusion in biosequence databases some of my specific concerns are as follows do you feel primers and probes tend to clutter up a nucleic acid database and cause unnecessary retrievals when searching for sequences of much longer length because primers and probes are usually designed from known sequence information often already in the database is it still justifiable to include them as additional entries what applications of primers and probes should justify their inclusion in a biosequence database e g clinical diagnosis taxonomy evolution gene mapping or methods if you were to search for primers and probes in a biosequence database what would be most efficient for you would some type of descriptive information be helpful e g their application or their origin th...
http://bio.net/bionet/mm/embl-db/1993-September/000224.html
*  RNAi
... jayanta tarafdar via methods net bio net by jayanta tcbckv from gmail com fri dec est previous message race sequencing problem next message a follow up question regarding dissolving h o insoluable compounds in dmso messages sorted by dear subscribers methods digest we have been working on the backcross breeding of rice with transgenic rice carrying rnai gene against virus would anybody help me regarding use of non radioactive probe for analysis of the transcript i would appreciate if anybody can give short cut method in this regard and urgently waiting for the answer jayanta india previous message race sequencing problem next message a follow up question regarding dissolving h o insoluable compounds in dmso messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/2008-December/103735.html
*  PT100 RTD Probes with M12 Molded Connectors
... Temperature Probes. The PR-22 Molded M12 RTD sensor can be used in a wide variety of applications as-is, or with one or more of Omega's In-Stock complimentary products such as: Extension Cables with silicone, polyurethane or PVC cables and straight or right angled connectors. Miniature Programmable Temperature Transmitter 4 to 20mA Output with M12 Connections Qty. Miniature Programmable Temperature Transmitter 0 to 10VDC Output with M12 Connections Qty. PVC Extension Cable, 5 Meters Long with Straight Female M12 Connector One End Qty. Polyurethane Extension Cable, 2 Meters Long with Straight Female M12 Connector One End Qty. Polyurethane Extension Cable, 5 Meters Long with Straight Female M12 Connector One End Qty. Silicone Extension Cable, 3 Meters Long with Straight Female M12 Connector One End Qty. Silicone Extension Cable, 5 Meters Long with Straight Female M12 Connector One End Qty. 1 Element Specification select from: 100-A for Pt100 Class A 1000-A for Pt1000 Class A 100-B for Pt100 Class B 2 Probe...
http://omega.com/pptst/PR-22.html
*  SS DNA probe length
... from johnson teri hello again i have a researcher who has made a digoxygenin labeled single stranded dna probe that is bp i am having trouble getting it to work on tissue sections negative results we have done a dot blot test so we know that the dig biotinylated anti dig streptavidin ap and substrate are working could it be this is just too big to penetrate into the tissue sections we digested the tissue with proteinase k acetylated with acetic anhydride in tea buffer hybridized overnight at degrees c and used the innogenex universal ish kit for dig labeled probes for detection thanks in advance teri johnson managing director histology facility stowers institute for medical research e th st kansas city missouri tjj stowers institute org previous message next message...
http://histosearch.com/histonet/Apr03/SSDNAprobelength.html
*  Gram-Specific DNA probes
gram specific dna probes gram specific dna probes mark fuller mark fuller at qmgate anl gov fri jan est previous message u cell line next message gram specific dna probes messages sorted by dear colleagues is anyone aware of any oligonucleotide probes that are specific for gram negative and gram positive eubacteria respectively any suggestions would be much appreciated mark fuller ph d environmental research division er j argonne national laboratory argonne il phone fax email mark fuller at qmgate anl gov previous message u cell line next message gram specific dna probes messages sorted by more information about the microbio mailing list...
http://bio.net/bionet/mm/microbio/1996-January/004227.html
*  GeneChip Hybridization Oven 645 from Affymetrix : Get Quote, RFQ, Price or Buy
... News A-Z. Health A-Z. Clinical Diagnostics. Life Science Laboratory. Consumer Products. Medical News 'Tweets'. Health. News. Life Science Laboratory. Laboratory. Hybridization Ovens. GeneChip Hybridization Oven 645 from Affymetrix. The GeneChip Hybridization Oven 645 provides precise temperature and rotation control to ensure the successful hybridization of as many as 64 GeneChip -brand cartridges at one time. An ergonomic front-panel display contains system status indicators and buttons for adjusting the set point temperature and the rotisserie speed. This panel, in addition to a highly visible display and large window, provides immediate feedback of proper operation when using the oven. Array Capacity: The oven holds up to eight removable probe array carriers. Each carrier holds up to eight arrays, enabling up to 64 arrays to be processed at one time. Removable Probe Array Carriers: The removable probe array carriers allow for the addition or removal of sets of probe arrays with minimal disruption to t...
http://news-medical.net/GeneChip-Hybridization-Oven-645-from-Affymetrix
*  Angle Beam / Wedge Transducer Nondestructive Testing (NDT) Probes Datasheets | IHS Engineering360
Angle Beam / Wedge Transducer Nondestructive Testing NDT Probes Datasheets. IHS Engineering360. News & Analysis. Products & Suppliers. Standards Library. Reference Library. Engineering Community. Stay Informed. Free Registration. Products & Suppliers. News & Analysis. Products & Suppliers. Standards Library. Reference Library. Community. PRODUCTS SUPPLIERS. DATASHEETS. NONDESTRUCTIVE TESTING NDT PROBES. ANGLE BEAM / WEDGE TRANSDUCER. Other Nondestructive Testing NDT Probes. Application: Coating Thickness. Conductivity. Flaw / Crack Detection. Wall / Material Thickness. Weld Inspection. NDT Probe Technology: Eddy Current. Remote Field Testing. Ultrasonic. Array. Contact Transducer. Protected Element. Style / Type: Angled / Curved Tip. Dual Element. Pencil Probe. Surface Probe. TOFD / Diffracted Wave. Angle Beam / Wedge Transducer Nondestructive Testing NDT Probes Datasheets. View Datasheet. Angle Beam Probes -- 1.5L16-A4. from Olympus Scientific Solutions America Angle beam probes are used with a wedge to tran...
http://globalspec.com/ds/3074/areaspec/style_angle_beam
*  Putting DNA to Work - DNA Sequence - Probe the Sequence
... DNA Sequence. Inherited Diseases. DNA/Criminal Justice. DNA SEQUENCE. Probe The Sequence - Text Only Version The more letters in a probe, the fewer matches it will find in the genome. The goal in forensics, disease detection, and selecting desirable traits in crops is often to probe for the DNA sequences that make an organism, a specific trait, or an individual person unique. A variety of different techniques are used, but the goal is always the same: probe for specific DNA sequences. Finding a single, unique sequence of A's, T's, G's, and C's within an organism's genome often reveals something new about that organism. How can a unique sequence be found in a genome that is three billion letters long. Searching and comparing sequences is the answer. The more letters in a probe, the fewer matches it will find in the genome. In this activity the user is asked to enter a combination of 3 letters in a black box at the top of the screen and click Probe. The computer shows the number of matches. For example the...
http://koshland-science-museum.org/sites/all/exhibits/exhibitdna/seq03text.jsp
*  Putting DNA to Work - DNA Sequence - Probe the Sequence
... DNA Home. Introduction. DNA Sequence. Inherited Diseases. DNA/Criminal Justice. Improving Crops. Infectious Disease. DNA SEQUENCE. Probe The Sequence - Text Only Version The more letters in a probe, the fewer matches it will find in the genome. The goal in forensics, disease detection, and selecting desirable traits in crops is often to probe for the DNA sequences that make an organism, a specific trait, or an individual person unique. A variety of different techniques are used, but the goal is always the same: probe for specific DNA sequences. Finding a single, unique sequence of A's, T's, G's, and C's within an organism's genome often reveals something new about that organism. How can a unique sequence be found in a genome that is three billion letters long. Searching and comparing sequences is the answer. The more letters in a probe, the fewer matches it will find in the genome. In this activity the user is asked to enter a combination of 3 letters in a black box at the top of the screen and click Pro...
https://koshland-science-museum.org/sites/all/exhibits/exhibitdna/seq03text.jsp
*  Quintessence Publishing
... NEW Sign Up to Receive Quintessence Updates by Email. Quintessence Publishing : Journals : OBM. Oral Biosciences & Medicine. Edited by Birgitte Nauntofte, Jesper Reibel. Peter A. Reichart, Jim Sciubba, and Joanna M. Zakrzewska Official publication of the European Society for Oral Laser Applications ISSN 1742-3287. Publication: Oral Biosciences & Medicine Year 2005 Volume 2, Issue 2. Back. Pages: 209 - 213. Etiology of Oral Disease in View of Microbial Complexity. Tanner, Anne C. R./Izard, Jacques. The number of different microorganisms recognized in the oral cavity using molecular methods has more than doubled compared with the number isolated using cultural techniques. This finding necessitates a reevaluation of which species may be pathogens in dental infections. . These molecular methods have clarified and expanded the taxonomy of oral microbial species. Discrepancies between comprehensive molecular and cultural methods suggest that neither method alone can adequately evaluate associations of specific...
http://quintpub.com/journals/obm/archive_display_abstract.php3?journalArt=7471
*  cwg probe Exclusive: Latest News on cwg probe Exclusive, Read Breaking News on IBNLive.com
... . Read. Watch. movies. Cricket. Tech. Follow Us. News. cj. compare india. property. states. Updated 8:29 pm Oct 6, 2015. English hindi marathi. Read. Watch. movies. Cricket. Tech. Latest Bihar Elections Politics India Sports Football Lifestyle Live TV Buzz. Menu. politics. india. blogs. photos. movies. tech. videos. Cricket. World. footballnext. Auto. Business. Books. Buzz. Lifestyle. Shows. cwg probe. ALL. NEWS. PHOTOS. VIDEOS. Anytime. Last 24 Hours. Past 4 Days. Past Week. Past Month. Sort by :. Date. . October 12, 2011, 4:55 pm. UK pushes for speedy conclusion of CWG probe. July 26, 2011, 2:58 pm. Is Kalmadi faking dementia to duck CWG probe. May 29, 2011, 9:06 am. London businessman not helping in CWG probe. April 11, 2011, 11:01 am. Shunglu report: PMO seeks Home Min's response. March 31, 2011, 6:03 pm. Shunglu report: BJP wants Dikshit to resign. March 29, 2011, 9:21 am. Shunglu panel conducted CWG probe, blames Kalmadi. March 15, 2011, 8:54 pm. New leads in CWG probe; CBI questions Kalmadi. March...
http://ibnlive.com/newstopics/CWG-probe.html
*  [alsa-devel] [PATCHv2 1/3] ASoC: codec: Simplify ASoC probe code.
ASoC: codec: Simplify ASoC probe code. ASoC: codec: Simplify ASoC probe code. Li.Xiubo at freescale.com Li.Xiubo at freescale.com. Mon Mar 3 04:01:52 CET 2014. Previous message: ASoC: codec: Simplify ASoC probe code. Next message: ASoC: codec: Simplify ASoC probe code. Messages sorted by:. Just removing the set cache io call will not work for all drivers. There are some MFD child devices which use regmap from the parent device. So dev get regmap will return NULL for those. This is the sort of thing that I was referring to when talking about doing the non-boring drivers separately. As well as the warnings Lars mentioned there's a bisection issue here: - codec- control data = da7213- regmap; - ret = snd soc codec set cache io codec, 8, 8, SND SOC REGMAP ; - if ret 0 { - dev err codec- dev, Failed to set cache I/O: %d\n, ret ; - return ret; - } - /* Default to using ALC auto offset calibration mode. */ snd soc update bits codec, DA7213 ALC CTRL1, DA7213 ALC CALIB MODE MAN, 0 ; Unless the core sets up the I/O bef...
http://mailman.alsa-project.org/pipermail/alsa-devel/2014-March/073679.html
*  Tomographic atom probe
... redirect atom probe atom probe tomography apt...
https://en.wikipedia.org/wiki/Tomographic_atom_probe
*  Biology help: Viral DNA incorporated into host cell DNA is known as a(n)?
Biology help: Viral DNA incorporated into host cell DNA is known as a n. Biology help: Viral DNA incorporated into host cell DNA is known as a n. 2.Viral DNA incorporated into host cell DNA is known as a n a.capsid. 3.Which of the following statements about plant viruses is false. a.To infect a plant, a virus must first get past the plant s epidermis. 4.A friend accidentally sends an email to you that contains a computer virus from his computer. Without knowing it, you infect your computer with the virus when you open the email. 6.How would the shape of a DNA molecule change if adenine paired with guanine and cytosine paired with thymine. 1.Consider the following sentence: The dog did not eat. 2.Viral DNA incorporated into host cell DNA is known as a n d. 3.Which of the following statements about plant viruses is false. a.To infect a plant, a virus must first get past the plant s epidermis. 4.A friend accidentally sends an email to you that contains a computer virus from his computer. Without knowing it, you ...
http://superbhostingnow.com/biology-help-viral-dna-incorporated-into-host-cell-dna-is-known-as-an/
*  Virus
... es can infect all types of organisms, from animals and plants to bacteria and archaea. The range of host cells that a virus can infect is called its " host range". Proteins associated with nucleic acid are known as nucleoproteins, and the association of viral capsid proteins with viral nucleic acid is called a nucleocapsid. Property Parameters Nucleic acid DNA RNA Both DNA and RNA at different stages in the life cycle. A virus has either DNA or RNA genes and is called a DNA virus or a RNA virus, respectively. Plant viruses tend to have single-stranded RNA genomes and bacteriophages tend to have double-stranded DNA genomes. A viral genome, irrespective of nucleic acid type, is almost always either single-stranded or double-stranded. For most viruses with RNA genomes and some with single-stranded DNA genomes, the single strands are said to be either positive-sense called the plus-strand or negative-sense called the minus-strand , depending on whether or not they are complementary to the viral messenger RNA ...
http://schools-wikipedia.org/wp/v/Virus.htm
*  Differential Diagnosis For HIV/Viral RNA/Plasma copies/quantitative (Lab) - Increased
Differential Diagnosis For HIV/Viral RNA/Plasma copies/quantitative Lab - Increased. English. Fran ais. Espa ol. Sign-in. Register. Differential Diagnosis. Diseases. Drugs. Tips. Try building your search one term at a time, and be as specific as you can. Search term example: "chronic cough". Do not enter multiple findings such as "anemia, chronic cough, weight loss, vomiting" all at the same time. After selecting your term from the search results a list of possible diagnoses will be generated. If the list is too long, you will be able to narrow it down by entering additional terms. Clinical. Sign Symptoms. Lab Results. CT-Scan. Sign-in or register to check out the new features we've just launched. Differential Diagnosis For HIV/Viral RNA/Plasma copies/quantitative Lab - Increased. Ads. List of current finding s :. HIV/Viral RNA/Plasma copies/quantitative Lab - Increased. Add Another Finding. Suggest a Better Translation. Suggest a Reference or External Link. How Can We Make It Better. Print 9 possible diagnos...
http://en.diagnosispro.com/differential_diagnosis-for/hiv-viral-rna-plasma-copies-quantitative-lab-increased/12519-154.html
*  DNA condensation
... Many features of the DNA double helix contribute to its large stiffness, including the mechanical properties of the sugar-phosphate backbone, electrostatic repulsion between phosphate s DNA bears on average one elementary negative charge per each 0.17 nm of the double helix, stacking interactions between the bases of each individual strand, and strand-strand interactions. DNA condensation in viruses DNA condensation in bacteria DNA condensation in eukaryotes DNA condensation 'in vitro' Physics of DNA condensation DNA condensation and gene regulation References Further reading. DNA condensation in viruses. Double-stranded DNA is stored inside the capsid in the form of a spool, which can have different types of coiling leading to different types of liquid-crystalline packing. Bacterial DNA is packed with the help of polyamines and proteins. Protein-associated DNA occupies about 1/4 of the intracellular volume forming a concentrated viscous phase with liquid crystalline properties, called the nucleoid. Bact...
https://en.wikipedia.org/wiki/DNA_condensation
*  Psych Central - HIV inserts into human genome using a DNA-associated protein
... Quizzes News Experts Ask the Therapist Blogs Experts Daily News Research Updates World of Psychology Research Resources Find a clinical Trial Resources Forums Support Groups Find Help Ask the Therapist Drugs Medications Find a Therapist Psychotherapy 101 Forums Support Groups Take a Quiz Mood Tracker Pro. Home Conditions Quizzes Ask the Therapist Drugs Blogs News Research Resources Find Help Psychotherapy 101 Forums Support Groups Pro. HIV inserts into human genome using a DNA-associated protein. LEDGF, a human DNA-associated protein, is the first example of a cellular protein controlling the location of HIV integration in human cells. A human DNA-associated protein called LEDGF is the first such molecule found to control the location of HIV integration in human cells, according to a new study from researchers at the University of Pennsylvania School of Medicine. This study, published in this week's early online edition of Nature Medicine, describes the first clear target for modulating where viruses ins...
http://psychcentral.com/news/archives/2005-11/uops-hii112305.html
*  First Living Organism with ‘Artificial’ DNA | JD Journal
First Living Organism with Artificial DNA. JD Journal. JDJournal. Stay Connected. Newsletter Subscription. Enter your email address and start getting breaking law firm and legal news right now. Every Alert Alert once a day. Free Market Evaluation - Send us your resume and we will give you free feedback. Home. Breaking News Business News Crime Law School News Tech Science News World News. Suit Claims Man Fired for ‘Flatulence,’ Foul Stench. Peeple: The App No One Was Waiting For. Hillary Worker Violates Election Law with Trump Meme. Anti-Paparazzi Law Upheld In Justin Bieber Case. Jobs. Post Jobs. Career Know-How Career Resources Celebrity News Humor Lists World News. Home. Breaking News. Tech Science News. First Living Organism with Artificial DNA Legal Job Listings. Keyword:. Location:. See Job Result. First Living Organism with Artificial DNA View Count: 302. Scientists have created the first living organism containing artificial DNA. According to Fox News, the advance one day could lead to new antibiotics,...
http://jdjournal.com/2014/05/08/first-living-organism-with-artificial-dna/
*  Protein-DNA binding specificity predictions with structural models | The Baker Laboratory
Protein-DNA binding specificity predictions with structural models. The Baker Laboratory. Skip to Main Content Area. news research Members Past Members publications employment contact info Links. Protein-DNA binding specificity predictions with structural models. Title. Protein-DNA binding specificity predictions with structural models. Publication Type. Journal Article. Year of Publication. 2005. Authors. Morozov, A. V, Havranek J. J, Baker D., & Siggia E. D Journal. Nucleic acids research. Volume. 33. Issue. 18. Pagination. 5781-98. Date Published. 2005. ISSN. 1362-4962. Keywords. Base Sequence, Binding Sites, Collaborative Publication, Computational Biology, Consensus Sequence, DNA, DNA-Binding Proteins, Models, Chemical, Nucleic Acid Conformation, Protein Binding, Transcription Factors. Abstract. Protein-DNA interactions play a central role in transcriptional regulation and other biological processes. Investigating the mechanism of binding affinity and specificity in protein-DNA complexes is thus an impor...
http://bakerlab.org/static/node/97/
*  New life engineered with artificial DNA - CNN.com
... Breaking News. Watch Live TV. Edition. News. Health. Living. Travel. Watch Live TV. CNNgo. Must Watch Videos. Watch Live TV. TV. CNNgo. Faces of CNN Worldwide. Watch Live TV. Social Commentary. Watch Live TV. Investigations. CNN profiles A-Z. CNN Leadership. Watch Live TV. Investigations. CNN profiles A-Z. CNN Leadership. New life engineered with artificial DNA. Scientists put X-Y base pair in DNA They got bacteria to replicate with unnatural genetics This is a brand new way of storing genetic information. Now, for the first time, scientists have shown it is possible to alter that alphabet and still have a living organism that passes on the genetic information. The findings also suggest that DNA as we know it on Earth may not be the only solution to coding for life, Romesberg said. FDA considers three-parent DNA procedure. MUST WATCH. FDA considers three-parent DNA procedure 03:29. MUST WATCH. Artist creates faces from human DNA. MUST WATCH. Artist creates faces from human DNA 01:33. For their genetic ex...
http://cnn.com/2014/05/09/health/artificial-dna-life/index.html
*  dna size
... richard p grant r grant at see sig for address tue sep est previous message dna size next message dna size messages sorted by in article s aq nuq at journal concentric net akb abansal at concentric net wrote why should i use the average aa weight do most proteins have a somewhat even distribution of light and heavy aa becuase it s an average what if this protein were made of nothing but gly besides the beginning met that could change the calculation yah and it d never fold and if it did it sure as heck wouldn t be soluble what if the protein were made of nothing but a heavy aa like trp i used ditto this method because since i want the minimum dna size then why not assume it is some weird protein with massive aa however i am not sure if this is the correct approach to take should i use method richard s or to be honest it depends what you want you could always assume that they re all huge and then that they re all tiny and get a range why are you doing this r sig removed for cleaning we apologize for any i...
http://bio.net/bionet/mm/cellbiol/1999-September/011499.html
*  Gibson assembly
... is a dna assembly method which allows for the joining of multiple dna fragments in a single isothermal reaction it was invented in by daniel gibson while he was at the j craig venter institute jcvi process thumb upright gibson assembly overview the entire gibson assembly reaction requires a small number of components with very few manipulations the method can simultaneously combine numerous dna fragments based on sequence identity it requires that the dna fragments contain base pair overlap with adjacent dna fragments these dna fragments are mixed with a cocktail of three enzymes along with other buffer components the three required enzyme activities are exonuclease dna polymerase and dna ligase the exonuclease chews back dna from the end the resulting single stranded regions on adjacent dna fragments can anneal the dna polymerase incorporates nucleotides to fill in any gaps the dna ligase covalently joins the dna of adjacent segments thereby removing any nicks in the dna the entire mixture is incubated ...
https://en.wikipedia.org/wiki/Gibson_assembly
*  Now® Grapeseed Oil - NNF - GNC
Now Grapeseed Oil - NNF - GNC. Save 15% Now. My Lists. Store Locator. Help. Order Status. Shipping Info. Return Policy. My Account. Customer Service. Log In or Register 0 Shopping Cart. There are no products in your Shopping Cart. *Please enter keyword or item # to search. Vitamins. Shop By Category. All Multivitamins. . Vitamins A-Z. Fish Oil & Omegas. Minerals. Whole Food Supplements. Specialty Supplements. Amino Acids. Antioxidants. Children and Teens. Men's Health. Women's Health. Promotions. Buy One, Get One 50% Off Vitapak Programs. Buy One, Get One 50% Off Select GNC Fish Oil. Popular Brands. GNC Womens Multivitamins. GNC Triflex. GNC Mens Multivitamins. GNC milestones. Tools & Resources. Get A Customized Vitamin Plan. Find Your Multivitamin. Find Your Vitapak Program. See All Resources. See All Videos. Sports Nutrition. Browse Categories. New Arrivals. Protein. Bars. Drinks/Hydration. Pre-Workout. During Workout. Post-Workout. Hardcore Products. Mass Gainers. Nitric Oxide. Creatine. Recovery. Amino Ac...
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*  Buy Authentic Discount GEO Olive Circle lenses or Color Lenses - US$18.80
... Color Circle Lenses GEO CTS / Olive Circle Color lens series. GEO CTS / Olive Circle Color lens series. Model: GEOOLIVE Manufactured by: Geo Medical Co. Select 0.00 -0.50 -1.00 -1.25 -1.50 -1.75 -2.00 -2.25 -2.50 -2.75 -3.00 -3.25 -3.50 -3.75 -4.00 -4.25 -4.50 -4.75 -5.00 -5.25 -5.50 -6.00 -6.50 -7.00 -7.50 -8.00 -8.50 -9.00 -9.50 -10.00 8.40-8.60mm 14.00/14.20 Select HC 104 HC 105 OL101 Purple Wing OL102 Blue Wing OL104 Honey Wing OL105 Ash Wing 0 1 2 3 4 5 6 7 8 9 10 11 12. Select 0.00 -0.50 -1.00 -1.25 -1.50 -1.75 -2.00 -2.25 -2.50 -2.75 -3.00 -3.25 -3.50 -3.75 -4.00 -4.25 -4.50 -4.75 -5.00 -5.25 -5.50 -6.00 -6.50 -7.00 -7.50 -8.00 -8.50 -9.00 -9.50 -10.00 8.40-8.60mm 14.00/14.20 Select HC 104 HC 105 OL101 Purple Wing OL102 Blue Wing OL104 Honey Wing OL105 Ash Wing 0 1 2 3 4 5 6 7 8 9 10 11 12. Geo CTS Color Olive series Circle Lenses :. Note : CTS / Olive Series / Harricane series. CTS series has now replaced with Olive series. GEO CTS OliveSeries Circle Lenses - 2 Tone Geo CTS Color Olive series Cir...
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*  Hyperchromicity
... thumb|right|250px|Nucleic acid melting curve showing hyperchromicity as a function of temperature 'Hyperchromicity' is the increase of absorbance 'optical density' of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level. Heat denaturation of DNA, also called melting, causes the double helix structure to unwind to form single stranded DNA. When DNA in solution is heated above its melting temperature usually more than 80 °C, the double-stranded DNA unwinds to form single-stranded DNA. The bases become unstacked and can thus absorb more light. In their native state, the bases of DNA absorb light in the 260-nm wavelength region. When the bases become unstacked, the wavelength of maximum absorbance does not change, but the amount absorbed increases by 37%. A double strand DNA dissociating to sing...
https://en.wikipedia.org/wiki/Hyperchromicity
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pr%%>%%>%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pres%25%25%3e%25%25%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Prese%%>%>%%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presen>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Present%>%,Present%>%,Present%>%,Present%>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentat%2%>%>%>%>%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentation>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnp%%>%%>%>%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
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*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnp%>%%3%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublishe%%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%>%>%>%%>%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presresen%3e%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=http:.В  They%>%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=http:.Р’В  They%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=pr%252525%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=pres%%%>%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presen%%%>%>,presen%%%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presentationunp%%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presentationunp%%%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pr%%%3e%>%%%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pr%25%25%3e%25%25%25%3e%25%25%3e
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pre%%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pres%%,Presen%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Pres%>%>% %>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Prese%%%%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presen%%%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presen%>%>%>%>%>%%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presen%25%25%25%2cPresen%25%25%25%25%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentat,Presentat%%%%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentatio%%%>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentatio%>%>%%%>,pres%%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presentation%25%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnp%%3e%%3e%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnp%2525%253e%2525%25253%2525%2525%253e%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%%>%>%>%>%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%%3e%%3e
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%%3e%%3e%%3e%%%3e%%%3e
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%%>%>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%%%>%>%>%>%>%>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%>%>%>%%%>%>%>%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%>%>%>%>%>%%>%>%%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%>%>%2
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%2%>%>%>%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=PresentationUnpublished%25%3e%25%3e%25%3e%25%3e%252%25%3e%25
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=Presresen>%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=http%253a%2525%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=pr%252525%%%>%>%>%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presen%%%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presentati%%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/?WBCMODE=presentationunpublished%>%%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/overview.htm?WBCMODE=Pr%%3E,Pr%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/overview.htm?WBCMODE=Pres%%,Presen%
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/overview.htm?WBCMODE=PresentationUnp%%3e%%3e%>%>
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/overview.htm?WBCMODE=PresentationUnpublished%252
*  School Health Services
... MSDE Home. Overview. Special Education and Early Intervention. Student, Family, and School Support. Division of Student, Family, and School Support Overview. Youth Development. Student Services and Alternative Programs.  . Alternative Programs.  .  .  .  . HIV/AIDS Prevention.  .  .  .  . Maryland Safe and Supportive Schools Grant.  .  .  .  . School-Based Health Centers.  . School Counseling.  . School Health Services.  . Overview.  . School Health Services Guidelines.  . School Mental Health.  . School Psychological Services.  .  .  .  .  . Teen Pregnancy.  .  . Overview. Divisions > Student, Family, and School Support > Student Services and Alternative Programs > School Health Services. Overview. CMT Training Program for Schools Only. School Immunization Requirements. Back to School Child Health Tips for Parents. Click here. Click here. Staph Infections and Schools - Information from the Department ...
http://marylandpublicschools.org/MSDE/divisions/studentschoolsvcs/student_services_alt/school_health_services/overview.htm?WBCMODE=presentationunp%>%>%>%%%%>%>

DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Hyperchromicity: Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured.Nucleic acid secondary structure: The secondary structure of a nucleic acid molecule refers to the basepairing interactions within a single molecule or set of interacting molecules, and can be represented as a list of bases which are paired in a nucleic acid molecule.Q-FISH: Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.Allele-specific oligonucleotide: An allele-specific oligonucleotide (ASO) is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay.Biotin sulfoxideTransfer-messenger RNA: Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1.Parenteral nutrition: Parenteral nutrition (PN) is feeding a person intravenously, bypassing the usual process of eating and digestion. The person receives nutritional formulae that contain nutrients such as glucose, amino acids, lipids and added vitamins and dietary minerals.Tritium illumination: Tritium illumination is the use of gaseous tritium, a radioactive isotope of hydrogen, to create visible light. Tritium emits electrons through beta decay, and, when they interact with a phosphor material, fluorescent light is created, a process called radioluminescence.TriprolidineLigation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Scavenger receptor: Scavenger receptors are a group of receptors that recognize modified low-density lipoprotein (LDL) by oxidation or acetylation. This naming is based on a function of cleaning (scavenging): Scavenger receptors widely recognize and take up macromolecules that have a negative charge, like modified LDL.Anterior pituitary: A major organ of the endocrine system, the anterior pituitary (also called the adenohypophysis or pars anterior), is the glandular, anterior lobe that together with the posterior lobe (posterior pituitary, or the neurohypophysis) makes up the pituitary gland (hypophysis). The anterior pituitary regulates several physiological processes including stress, growth, reproduction and lactation.Restriction fragment: A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.Glutathionuria: Glutathionuria is the presence of glutathione in the urine, and is a rare inborn error of metabolism.Hybrid inviability: Hybrid inviability is a post-zygotic barrier, which reduces a hybrid's capacity to mature into a healthy, fit adult.Hybrid inviability.Lewis H. Brown: Lewis Brown}}Nucleic acid structure: Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar.YjdF RNA motifPolymethine: Polymethines are compounds made up from an odd number of methine groups (CH) bound together by alternating single and double bonds.Kachovski and Dekhtyar, Dyes and Pigments, 22 (1983) 83-97 Compounds made up from an even number of methine groups are known as polyenes.Mature messenger RNA: Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, it consists exclusively of exons, with all introns removed.Cytoplasmic male sterility: Cytoplasmic male sterility is total or partial male sterility in plants as the result of specific nuclear and mitochondrial interactions. Male sterility is the failure of plants to produce functional anthers, pollen, or male gametes.Platelet-poor plasma: Platelet-Poor Plasma (PPP) is blood plasma with very low number of platelets (< 10 X 103/μL). Traditionally, PPP was recommended for use in platelet aggregation studies to both adjust the Platelet-rich plasma concentration, and to serve as a control.Buoyant density ultracentrifugation: Buoyant density centrifugation uses the concept of buoyancy to separate molecules in solution. Usually a caesium chloride (CsCl) solution is used, but in the general case it's usually approximately the same density as the molecules that are to be centrifuged.Triparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.Assay sensitivity: Assay sensitivity is a property of a clinical trial defined as the ability of a trial to distinguish an effective treatment from a less effective or ineffective intervention. Without assay sensitivity, a trial is not internally valid and is not capable of comparing the efficacy of two interventions.Eukaryotic transcription: Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Selective internal radiation therapy: Selective internal radiation therapy (SIRT) is a form of radiation therapy used in interventional radiology to treat cancer. It is generally for selected patients with unresectable cancers, those that cannot be treated surgically, especially hepatic cell carcinoma or metastasis to the liver.Fishpaper: Fish paper or fishpaper is a strong, flexible, fibrous dielectric paper. It resists moderate heat and mechanical injury, and is often used for wrapping coils and insulating stove-top parts.Permissive temperature: The permissive temperature is the temperature at which a temperature sensitive mutant gene product takes on a normal, functional phenotype.http://www.Burst kinetics: Burst kinetics is a form of enzyme kinetics that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase".Michael A. EpsteinGoitreRiboprobe: Riboprobes are RNA probes that can be produced by in vitro transcription of cloned DNA inserted in a suitable plasmid downstream of a viral promoter. Some bacterial viruses code for their own RNA polymerases, which are highly specific for the viral promoters.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.

(1/18626) Identification and characterization of the human orthologue of yeast Pex14p.

Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders.  (+info)

(2/18626) The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells.

PINCH is a widely expressed and evolutionarily conserved protein comprising primarily five LIM domains, which are cysteine-rich consensus sequences implicated in mediating protein-protein interactions. We report here that PINCH is a binding protein for integrin-linked kinase (ILK), an intracellular serine/threonine protein kinase that plays important roles in the cell adhesion, growth factor, and Wnt signaling pathways. The interaction between ILK and PINCH has been consistently observed under a variety of experimental conditions. They have interacted in yeast two-hybrid assays, in solution, and in solid-phase-based binding assays. Furthermore, ILK, but not vinculin or focal adhesion kinase, has been coisolated with PINCH from mammalian cells by immunoaffinity chromatography, indicating that PINCH and ILK associate with each other in vivo. The PINCH-ILK interaction is mediated by the N-terminal-most LIM domain (LIM1, residues 1 to 70) of PINCH and multiple ankyrin (ANK) repeats located within the N-terminal domain (residues 1 to 163) of ILK. Additionally, biochemical studies indicate that ILK, through the interaction with PINCH, is capable of forming a ternary complex with Nck-2, an SH2/SH3-containing adapter protein implicated in growth factor receptor kinase and small GTPase signaling pathways. Finally, we have found that PINCH is concentrated in peripheral ruffles of cells spreading on fibronectin and have detected clusters of PINCH that are colocalized with the alpha5beta1 integrins. These results demonstrate a specific protein recognition mechanism utilizing a specific LIM domain and multiple ANK repeats and suggest that PINCH functions as an adapter protein connecting ILK and the integrins with components of growth factor receptor kinase and small GTPase signaling pathways.  (+info)

(3/18626) Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis.

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval.  (+info)

(4/18626) Single atom modification (O-->S) of tRNA confers ribosome binding.

Escherichia coli tRNALysSUU, as well as human tRNALys3SUU, has 2-thiouridine derivatives at wobble position 34 (s2U*34). Unlike the native tRNALysSUU, the full-length, unmodified transcript of human tRNALys3UUU and the unmodified tRNALys3UUU anticodon stem/loop (ASLLys3UUU) did not bind AAA- or AAG-programmed ribosomes. In contrast, the completely unmodified yeast tRNAPhe anticodon stem/loop (ASLPheGAA) had an affinity (Kd = 136+/-49 nM) similar to that of native yeast tRNAPheGmAA (Kd = 103+/-19 nM). We have found that the single, site-specific substitution of s2U34 for U34 to produce the modified ASLLysSUU was sufficient to restore ribosomal binding. The modified ASLLysSUU bound the ribosome with an affinity (Kd = 176+/-62 nM) comparable to that of native tRNALysSUU (Kd = 70+/-7 nM). Furthermore, in binding to the ribosome, the modified ASLLys3SUU produced the same 16S P-site tRNA footprint as did native E. coli tRNALysSUU, yeast tRNAPheGmAA, and the unmodified ASLPheGAA. The unmodified ASLLys3UUU had no footprint at all. Investigations of thermal stability and structure monitored by UV spectroscopy and NMR showed that the dynamic conformation of the loop of modified ASLLys3SUU was different from that of the unmodified ASLLysUUU, whereas the stems were isomorphous. Based on these and other data, we conclude that s2U34 in tRNALysSUU and in other s2U34-containing tRNAs is critical for generating an anticodon conformation that leads to effective codon interaction in all organisms. This is the first example of a single atom substitution (U34-->s2U34) that confers the property of ribosomal binding on an otherwise inactive tRNA.  (+info)

(5/18626) Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells.

Accumulation of mesangial matrix is a pivotal event in the pathophysiology of diabetic nephropathy. The molecular triggers for matrix production are still being defined. Here, suppression subtractive hybridization identified 15 genes differentially induced when primary human mesangial cells are exposed to high glucose (30 mM versus 5 mM) in vitro. These genes included (a) known regulators of mesangial cell activation in diabetic nephropathy (fibronectin, caldesmon, thrombospondin, and plasminogen activator inhibitor-1), (b) novel genes, and (c) known genes whose induction by high glucose has not been reported. Prominent among the latter were genes encoding cytoskeleton-associated proteins and connective tissue growth factor (CTGF), a modulator of fibroblast matrix production. In parallel experiments, elevated CTGF mRNA levels were demonstrated in glomeruli of rats with streptozotocin-induced diabetic nephropathy. Mannitol provoked less mesangial cell CTGF expression in vitro than high glucose, excluding hyperosmolality as the key stimulus. The addition of recombinant CTGF to cultured mesangial cells enhanced expression of extracellular matrix proteins. High glucose stimulated expression of transforming growth factor beta1 (TGF-beta1), and addition of TGF-beta1 to mesangial cells triggered CTGF expression. CTGF expression induced by high glucose was partially suppressed by anti-TGF-beta1 antibody and by the protein kinase C inhibitor GF 109203X. Together, these data suggest that 1) high glucose stimulates mesangial CTGF expression by TGFbeta1-dependent and protein kinase C dependent pathways, and 2) CTGF may be a mediator of TGFbeta1-driven matrix production within a diabetic milieu.  (+info)

(6/18626) Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20-prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.  (+info)

(7/18626) The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.

The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.  (+info)

(8/18626) Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively.

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)


The lac regulatory system is important to bacteria because the sugar lactose?


(continue)
The lac regulatory system is important to bacteria because the sugar lactose

a. cannot be made by bacteria unless the genes are turned on. 
b. is the most common source of food; enzymes are needed all the time. 
c. is only rarely available; producing enzymes all the time is costly. 
d. is incorporated into the nucleic acid of the bacteria. 
e. switches the system off and on whether lactose is present or not.
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b. is the most common source of food; enzymes are needed all the time.


How does the immune system remember specific pathogens ?


Do they recognise them by their protein coat or capsid or do they remember their nucleic acid material ie DNA or RNA ? Once they are broken down ?
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It's all in the antigens, my friend.


Why does the body catabolize fats and carbs before proteins?


It's a question for my bio assignment: 

"When an organism is deprived of food for a long time, it catabolizzes its bodily stores of fats, carbohydrates, proteins and nucleic acids for energy. Why are fats and carbohydrates used for this purpose before proteins and nucleic acids?"
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Energy; deprived of food, needs energy; gram of fat = 9 cal; carb and protein: 4.


What is low-acid coffee, how do they make it, and what are the best brands?


I've heard of low-acid coffee and I think it might help with my stomach problems. I'm wondering what it is exactly, how they make it, and which brands are considered to be the best. Thanks!
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When coffee beans are roasted, acid is extraced due to the heat inside the roasters themselves. One of the most popular ways to take the acid down, is to put the beans through a steaming process that eliminates most of the acid or by roasting the beans to be as dark as humanly possible without buring them (darker roasts tend to have less acid, believe it or not). Recently, there was a company that said it had the lowest acididity rate on the market called Puroast. I haven't hear any of their cupping reviews, however. 

Finding a good, low-acid coffee might be harder to find on the normal market, I fear. Roasters are just now starting to look at the growing numbers of people who need this kind of brew. From everything I have read, Brazilian and Sumatrian beans look like they are going to be able to deliver low-acid with great taste. You do have to realise, however, that this is still a work in progress for the coffee industry. You are going to need to give us some time. I do, however have a process you can do at home to lower the acidity in your coffee. The downside is that it is for iced coffee, and I only know how to make it in kind of a large dose (but it keeps for quite a while). Let me know if you want it!


How do I prepare Salicylic Acid in powder form to use as an acne cure?


I have purchased Salicylic Acid, but unfortunately has come with health warning and no instructions for how to use or what mix is necessary.  Please does anyone know what the spell/recipe is for acne?
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Salicylic acid is essentially aspirin. I have taken an aspirin, crushed it into the powder, added a little water to make a not too thick or not too thin paste, then put it on a blemish, top it with a "spot" bandage and let it work over night. It works fine and I have had no reactions, however some people with sensitive skin may react...it is on the acidic side of the pH scale, although not as strong as hydrochloric acid, I imagine it could cause some irritation. If you get irritation, try it during the day for only a few hours. Good luck!


How long does it take for acid reflux in babies to go away?


My 2 month old baby has acid reflux. He is on Prevacid and Mylanta. I burp him and hold him upright for 30 mins after each meal. Question for all parents whose babies have had acid reflux: How long did it take for your babies to get rid off their acid reflux ?
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My son has acid reflux.... He also has tracheomalacia (weakened trachea) He was on hypoallergenic formula... I didnt put him on zantac or prevacid or anything like that... the dr did suggest putting some cereal in the bottles, but I didnt want to do that either..

He is 6 months old now and is pretty much done with the whole acid reflux thing... He spits up sometimes, but not like he used to. We switched him back over to milk based formula when he started solids (around 5 months) and he has been good ever since.

I think it is really a baby by baby thing... each baby just has to grow out of it on their own.

Good luck.


What can sulfuric acid do to your skin after prolonged exposure?


I have a friend who spilled sulfuric acid on his arm at work and he won't go to the hospital. He now has a hole in his arm.
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Sulfuric acid is a strong Acid if undiluted (0 on ph scale) and would not just, "leave a pale spot" because it is extremely corrosive. I've learned from a man who worked in some plants It eats through the tissue until it loses surface tension of your appendage and falls to further eat through most floors until in safe containment, IE Metals that cannot be corroded away.


How does adding an acid to vegetable cooking water affect the vegetable?


I want to know what will happen when you add acid to different types of vegetables -- green, red, orange, white. Also, how can you artificially enhance or diminish the acid-pigment interaction in an aqueous (that's water) environment. Explain the science involved in detail.
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the only thing i can think of is an acid would help keep certain vegetables from oxidizing or changing color like eggplant. it also acts as a preservative for many vegetables depending on how much acid you use or mix with water u can go as far as pickling a vegetable.
it also keeps a veggey like fresh artichokes from turning brown in a solution of water and a little acid