Multilocus Sequence Typing: Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Molecular Epidemiology: The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Genetic Variation: Genotypic differences observed among individuals in a population.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Molecular Typing: Using MOLECULAR BIOLOGY techniques, such as DNA SEQUENCE ANALYSIS; PULSED-FIELD GEL ELECTROPHORESIS; and DNA FINGERPRINTING, to identify, classify, and compare organisms and their subtypes.Mycological Typing Techniques: Procedures for identifying types and strains of fungi.Campylobacter jejuni: A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.Bacterial Proteins: Proteins found in any species of bacterium.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Staphylococcal Infections: Infections with bacteria of the genus STAPHYLOCOCCUS.Campylobacter Infections: Infections with bacteria of the genus CAMPYLOBACTER.Campylobacter coli: A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic.Genes, Bacterial: The functional hereditary units of BACTERIA.Minisatellite Repeats: Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Carrier State: The condition of harboring an infective organism without manifesting symptoms of infection. The organism must be readily transmissible to another susceptible host.Alleles: Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Methicillin Resistance: Non-susceptibility of a microbe to the action of METHICILLIN, a semi-synthetic penicillin derivative.Pneumococcal Infections: Infections with bacteria of the species STREPTOCOCCUS PNEUMONIAE.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Drug Resistance, Bacterial: The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Neisseria meningitidis: A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.Streptococcal Infections: Infections with bacteria of the genus STREPTOCOCCUS.Electrophoresis, Starch Gel: Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Amplified Fragment Length Polymorphism Analysis: The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments.Meningococcal Infections: Infections with bacteria of the species NEISSERIA MENINGITIDIS.Methicillin-Resistant Staphylococcus aureus: A strain of Staphylococcus aureus that is non-susceptible to the action of METHICILLIN. The mechanism of resistance usually involves modification of normal or the presence of acquired PENICILLIN BINDING PROTEINS.beta-Lactamases: Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Cronobacter: A genus of gram-negative opportunistic foodborne pathogens.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Gram-Positive Bacterial Infections: Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method.Cross Infection: Any infection which a patient contracts in a health-care institution.Poultry: Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Ribotyping: RESTRICTION FRAGMENT LENGTH POLYMORPHISM analysis of rRNA genes that is used for differentiating between species or strains.Disease Outbreaks: Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.Achromobacter: A genus of gram-negative, strictly aerobic, non-spore forming rods. Soil and water are regarded as the natural habitat. They are sometimes isolated from a hospital environment and humans.Polymorphism, Genetic: The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.Leukocidins: Pore forming proteins originally discovered for toxic activity to LEUKOCYTES. They are EXOTOXINS produced by some pathogenic STAPHYLOCOCCUS and STREPTOCOCCUS that destroy leukocytes by lysis of the cytoplasmic granules and are partially responsible for the pathogenicity of the organisms.Enterococcus faecium: A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.Hospitals: Institutions with an organized medical staff which provide medical care to patients.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Sequence Homology: The degree of similarity between sequences. Studies of AMINO ACID SEQUENCE HOMOLOGY and NUCLEIC ACID SEQUENCE HOMOLOGY provide useful information about the genetic relatedness of genes, gene products, and species.Streptococcus agalactiae: A bacterium which causes mastitis in cattle and occasionally in man.Geography: The science dealing with the earth and its life, especially the description of land, sea, and air and the distribution of plant and animal life, including humanity and human industries with reference to the mutual relations of these elements. (From Webster, 3d ed)Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Genetics, Population: The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.Meningitis, Meningococcal: A fulminant infection of the meninges and subarachnoid fluid by the bacterium NEISSERIA MENINGITIDIS, producing diffuse inflammation and peri-meningeal venous thromboses. Clinical manifestations include FEVER, nuchal rigidity, SEIZURES, severe HEADACHE, petechial rash, stupor, focal neurologic deficits, HYDROCEPHALUS, and COMA. The organism is usually transmitted via nasopharyngeal secretions and is a leading cause of meningitis in children and young adults. Organisms from Neisseria meningitidis serogroups A, B, C, Y, and W-135 have been reported to cause meningitis. (From Adams et al., Principles of Neurology, 6th ed, pp689-701; Curr Opin Pediatr 1998 Feb;10(1):13-8)Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Burkholderia cepacia complex: A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.Community-Acquired Infections: Any infection acquired in the community, that is, contrasted with those acquired in a health care facility (CROSS INFECTION). An infection would be classified as community-acquired if the patient had not recently been in a health care facility or been in contact with someone who had been recently in a health care facility.Klebsiella pneumoniae: Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.Exotoxins: Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.Xylella: A genus of gram-negative, aerobic bacteria, in the family XANTHOMONADACEAE. It is found in the xylem of plant tissue.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.Spain: Parliamentary democracy located between France on the northeast and Portugual on the west and bordered by the Atlantic Ocean and the Mediterranean Sea.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.PortugalPoultry Diseases: Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild.Pets: Animals kept by humans for companionship and enjoyment, as opposed to DOMESTIC ANIMALS such as livestock or farm animals, which are kept for economic reasons.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Gene Transfer, Horizontal: The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).Campylobacter: A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.DNA, Concatenated: Head to tail array of covalently joined DNA sequences generated by concatenation. Concatenated DNA is attached end to end in contrast to CATENATED DNA which is attached loop to loop.Phylogeography: A field of study concerned with the principles and processes governing the geographic distributions of genealogical lineages, especially those within and among closely related species. (Avise, J.C., Phylogeography: The History and Formation of Species. Harvard University Press, 2000)Genomic Islands: Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands".Sinorhizobium: A genus of gram-negative, aerobic, nonsporeforming rods which usually contain granules of poly-beta-hydroxybutyrate. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Burkholderia pseudomallei: A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.Nerium: A plant genus of the family APOCYNACEAE. It is a very poisonous plant that contains cardioactive agents.Klebsiella Infections: Infections with bacteria of the genus KLEBSIELLA.Arcobacter: A genus of gram-negative, aerotolerant, spiral-shaped bacteria isolated from water and associated with diarrhea in humans and animals.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.beta-Lactam Resistance: Nonsusceptibility of bacteria to the action of the beta-lactam antibiotics. Mechanisms responsible for beta-lactam resistance may be degradation of antibiotics by BETA-LACTAMASES, failure of antibiotics to penetrate, or low-affinity binding of antibiotics to targets.Candida tropicalis: A species of MITOSPORIC FUNGI that is a major cause of SEPTICEMIA and disseminated CANDIDIASIS, especially in patients with LYMPHOMA; LEUKEMIA; and DIABETES MELLITUS. It is also found as part of the normal human mucocutaneous flora.Genes, Essential: Those genes found in an organism which are necessary for its viability and normal function.Bacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Animals, Domestic: Animals which have become adapted through breeding in captivity to a life intimately associated with humans. They include animals domesticated by humans to live and breed in a tame condition on farms or ranches for economic reasons, including LIVESTOCK (specifically CATTLE; SHEEP; HORSES; etc.), POULTRY; and those raised or kept for pleasure and companionship, e.g., PETS; or specifically DOGS; CATS; etc.Cattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Coxiellaceae: A family of gram-negative bacteria in the order Legionellales. It includes genera COXIELLA and Rickettsiella.Staphylococcus epidermidis: A species of STAPHYLOCOCCUS that is a spherical, non-motile, gram-positive, chemoorganotrophic, facultative anaerobe. Mainly found on the skin and mucous membrane of warm-blooded animals, it can be primary pathogen or secondary invader.Nose: A part of the upper respiratory tract. It contains the organ of SMELL. The term includes the external nose, the nasal cavity, and the PARANASAL SINUSES.Streptococcus suis: A species of STREPTOCOCCUS isolated from pigs. It is a pathogen of swine but rarely occurs in humans.Genetic Loci: Specific regions that are mapped within a GENOME. Genetic loci are usually identified with a shorthand notation that indicates the chromosome number and the position of a specific band along the P or Q arm of the chromosome where they are found. For example the locus 6p21 is found within band 21 of the P-arm of CHROMOSOME 6. Many well known genetic loci are also known by common names that are associated with a genetic function or HEREDITARY DISEASE.Methicillin: One of the PENICILLINS which is resistant to PENICILLINASE but susceptible to a penicillin-binding protein. It is inactivated by gastric acid so administered by injection.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.North AmericaLinkage Disequilibrium: Nonrandom association of linked genes. This is the tendency of the alleles of two separate but already linked loci to be found together more frequently than would be expected by chance alone.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Bacteremia: The presence of viable bacteria circulating in the blood. Fever, chills, tachycardia, and tachypnea are common acute manifestations of bacteremia. The majority of cases are seen in already hospitalized patients, most of whom have underlying diseases or procedures which render their bloodstreams susceptible to invasion.Mastitis, Bovine: INFLAMMATION of the UDDER in cows.Zoonoses: Diseases of non-human animals that may be transmitted to HUMANS or may be transmitted from humans to non-human animals.Random Amplified Polymorphic DNA Technique: Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.Neisseria meningitidis, Serogroup W-135: Strains of Neisseria meningitidis found mostly in Africa.Enterococcus faecalis: A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.Vibrio mimicus: A species of gram-negative bacteria in the genus VIBRIO, isolated from SHELLFISH, as well as from human diarrheal stools and ear infections.Melioidosis: A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.Vancomycin Resistance: Nonsusceptibility of bacteria to the action of VANCOMYCIN, an inhibitor of cell wall synthesis.Struthioniformes: An order of flightless birds comprising the ostriches, which naturally inhabit open, low rainfall areas of Africa.Multiplex Polymerase Chain Reaction: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Genetic Speciation: The splitting of an ancestral species into daughter species that coexist in time (King, Dictionary of Genetics, 6th ed). Causal factors may include geographic isolation, HABITAT geometry, migration, REPRODUCTIVE ISOLATION, random GENETIC DRIFT and MUTATION.Staphylococcal Protein A: A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.Burkholderia Infections: Infections with bacteria of the genus BURKHOLDERIA.Microsatellite Repeats: A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).Communicable Diseases, Emerging: Infectious diseases that are novel in their outbreak ranges (geographic and host) or transmission mode.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Cryptococcus gattii: A species of the fungus CRYPTOCOCCUS. Its teleomorph is Filobasidiella bacillispora.Pantoea: A genus of gram-negative, facultatively anaerobic, straight rods which are motile by peritrichous flagella. Most strains produce a yellow pigment. This organism is isolated from plant surfaces, seeds, soil, and water, as well as from animals and human wounds, blood, and urine. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Salmonella enterica: A subgenus of Salmonella containing several medically important serotypes. The habitat for the majority of strains is warm-blooded animals.Enterobacteriaceae Infections: Infections with bacteria of the family ENTEROBACTERIACEAE.Plant Diseases: Diseases of plants.Penicillin Resistance: Nonsusceptibility of an organism to the action of penicillins.DNA, Ribosomal Spacer: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Neisseria lactamica: A species of gram-negative, aerobic BACTERIA commonly found in the NASOPHARYNX of infants and children, but rarely pathogenic. It is the only species to produce acid from LACTOSE.Petroselinum: A plant genus of the family APIACEAE used for flavoring food.Streptococcus equi: A species of gram-positive, coccoid bacteria isolated from abscesses in submaxillary glands and mucopurulent discharges of the upper respiratory tract of horses. This organism belongs to Group C streptococci with regards to antigen response and is known to cause strangles. The subspecies S. zooepidemicus is also considered a pathogen of horses.Vibrio Infections: Infections with bacteria of the genus VIBRIO.DNA Gyrase: A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. Gyrase binds to DNA as a heterotetramer consisting of two A and two B subunits. In the presence of ATP, gyrase is able to convert the relaxed circular DNA duplex into a superhelix. In the absence of ATP, supercoiled DNA is relaxed by DNA gyrase.Streptococcus pyogenes: A species of gram-positive, coccoid bacteria isolated from skin lesions, blood, inflammatory exudates, and the upper respiratory tract of humans. It is a group A hemolytic Streptococcus that can cause SCARLET FEVER and RHEUMATIC FEVER.Leptospira: A genus of aerobic, helical spirochetes, some species of which are pathogenic, others free-living or saprophytic.Candidiasis: Infection with a fungus of the genus CANDIDA. It is usually a superficial infection of the moist areas of the body and is generally caused by CANDIDA ALBICANS. (Dorland, 27th ed)Czech Republic: Created 1 January 1993 as a result of the division of Czechoslovakia into the Czech Republic and Slovakia.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.China: A country spanning from central Asia to the Pacific Ocean.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Staphylococcus hominis: A species of STAPHYLOCOCCUS similar to STAPHYLOCOCCUS HAEMOLYTICUS, but containing different esterases. The subspecies Staphylococcus hominis novobiosepticus is highly virulent and novobiocin resistant.EuropeCandida albicans: A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).Genetic Markers: A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.South AmericaPrevalence: The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Neisseria meningitidis, Serogroup Y: Strains of Neisseria meningitidis which, in the United States, causes disease in mostly adults and the elderly. Serogroup Y strains are associated with PNEUMONIA.Turkeys: Large woodland game BIRDS in the subfamily Meleagridinae, family Phasianidae, order GALLIFORMES. Formerly they were considered a distinct family, Melegrididae.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Vibrio parahaemolyticus: A species of bacteria found in the marine environment, sea foods, and the feces of patients with acute enteritis.Australia: The smallest continent and an independent country, comprising six states and two territories. Its capital is Canberra.Prophages: Genomes of temperate BACTERIOPHAGES integrated into the DNA of their bacterial host cell. The prophages can be duplicated for many cell generations until some stimulus induces its activation and virulence.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Birds: Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.Medicago: A plant genus of the family FABACEAE. It is distinct from Sweet Clover (MELILOTUS), from Bush Clover (LESPEDEZA), and from Red Clover (TRIFOLIUM).Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Asia: The largest of the continents. It was known to the Romans more specifically as what we know today as Asia Minor. The name comes from at least two possible sources: from the Assyrian asu (to rise) or from the Sanskrit usa (dawn), both with reference to its being the land of the rising sun, i.e., eastern as opposed to Europe, to the west. (From Webster's New Geographical Dictionary, 1988, p82 & Room, Brewer's Dictionary of Names, 1992, p34)Streptococcus mitis: A species of gram-positive, coccoid bacteria commensal in the respiratory tract.Bartonella henselae: A species of gram-negative bacteria that is the etiologic agent of bacillary angiomatosis (ANGIOMATOSIS, BACILLARY). This organism can also be a cause of CAT-SCRATCH DISEASE in immunocompetent patients.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Abattoirs: Places where animals are slaughtered and dressed for market.Flagellin: A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.Enterohemorrhagic Escherichia coli: Strains of ESCHERICHIA COLI that are a subgroup of SHIGA-TOXIGENIC ESCHERICHIA COLI. They cause non-bloody and bloody DIARRHEA; HEMOLYTIC UREMIC SYNDROME; and hemorrhagic COLITIS. An important member of this subgroup is ESCHERICHIA COLI O157-H7.Bradyrhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.France: A country in western Europe bordered by the Atlantic Ocean, the English Channel, the Mediterranean Sea, and the countries of Belgium, Germany, Italy, Spain, Switzerland, the principalities of Andorra and Monaco, and by the duchy of Luxembourg. Its capital is Paris.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Campylobacter lari: A species of thermophilic CAMPYLOBACTER found in healthy seagulls and causing ENTERITIS in humans.Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Selection, Genetic: Differential and non-random reproduction of different genotypes, operating to alter the gene frequencies within a population.Carbon-Oxygen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. EC 6.1.GreeceDisease Reservoirs: Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.Rhizobiaceae: A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.Genes, Mating Type, Fungal: Fungal genes that mostly encode TRANSCRIPTION FACTORS. In some FUNGI they also encode PHEROMONES and PHEROMONE RECEPTORS. The transcription factors control expression of specific proteins that give a cell its mating identity. Opposite mating type identities are required for mating.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Infant, Newborn: An infant during the first month after birth.Erythromycin: A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.Kingella kingae: A species of gram-negative, aerobic, rod-shaped bacteria which is distinguished from other members of the genus KINGELLA by its beta hemolysis. It occurs normally in human mucous membranes of the upper respiratory tract, but can cause septic arthritis and endocarditis. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Salmonella Infections: Infections with bacteria of the genus SALMONELLA.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Thailand: Formerly known as Siam, this is a Southeast Asian nation at the center of the Indochina peninsula. Bangkok is the capital city.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Nasopharynx: The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.GermanyCandida glabrata: A species of MITOSPORIC FUNGI commonly found on the body surface. It causes opportunistic infections especially in immunocompromised patients.

*  JCVI: A Multilocus Sequence Typing System (MLST) Reveals a High Level of Diversity and a Genetic Com
JCVI: A Multilocus Sequence Typing System MLST Reveals a High Level ... WA, Caler E. A Multilocus Sequence Typing System MLST Reveals a ... An E. histolytica multilocus genotyping system based on tRNA gene- ... jcvi a multilocus sequence typing system mlst reveals a high level of diversity and a genetic component to entamoeba histolytica virulence home about research sustainable lab publications education giving press careers contact listing about publications citation gilchrist ca ali ik kabir m alam f scherbakova s ferlanti e weedall gd hall n haque r petri wa caler e a multilocus sequence typing system mlst reveals a high level of diversity and a genetic component to entamoeba histolytica virulence bmc microbiology dec external citation abstract the outcome of an entamoeba histolytica infection is variable and can result in either asymptomatic carriage immediate or latent disease diarrhea dysentery amebic liver abscess an e histolytica multilocus genotyping system based on trna gene linked arrays has s...
http://jcvi.org/cms/index.php?id=629&no_cache=1&tx_ttnews[tt_news]=3052/
*  High Genetic Diversity of Enterococcus faecium and Enterococcus faecalis Clinical Isolates by Pulsed
Electrophoresis and Multilocus Sequence Typing from a Hospital in ... genes Primer sequences 5′-3′. adk Forward...
http://hindawi.com/journals/bmri/2013/938937/tab1/
*  High Genetic Diversity of Enterococcus faecium and Enterococcus faecalis Clinical Isolates by Pulsed
Electrophoresis and Multilocus Sequence Typing from a Hospital in ... , Belgium. Pulsotype refers to subtypes labelled as 1, 2, 3 ... E. faecalis. ST type refers to the assigned sequence type...
http://hindawi.com/journals/bmri/2013/938937/fig1/
*  .. Tag Archives: Germany .. Just cook it doesn’t cut it: Egg factory owner in Germany arrested o
with XbaI, and multilocus sequence typing MLST were used to type ... Doug Powell. Reply A poultry farmer whose contaminated eggs caused a Salmonella outbreak at a Birmingham, UK hospital has been arrested over the death of a man in Austria. Posted in Food Safety Policy, Salmonella. Tagged Birmingham, Death, Egg, food safety, Germany, salmonella. Leave a reply. Doug Powell. Posted in Salmonella. Tagged food safety, Germany, salmonella, Sprouts, Switzerland. Leave a reply. Doug Powell. coli, Food Safety Policy. Tagged e coli, food safety, Germany, Sprouts, Stec. Leave a reply. Doug Powell. Reply I went to Germany a few years ago, and after hours on the train, really had to pee. Leave a reply. Doug Powell. Tagged Bakery, food safety, Germany, hepatitis A. Leave a reply. Reply The 38 human cases of monophasic Salmonella Typhimurium detected recently in six Member States are likely to be part of the same cluster, a joint EFSA/ECDC report has found. The recently detected 38 cases of monophasic Salmonella Typhimuri...
http://barfblog.com/tags/germany/
*  European Society of Clinical Microbiology and Infectious Diseases
knowledge of genome sequence. It combines the principle of ... , from 11 major multilocus sequence types, including 15 isolates ... using the ABI 3730 sequencer and GeneMapper software v.4.0 for data...
http://blackwellpublishing.com/eccmid20/abstract.asp?id=84799
*  Micromonospora fulviviridis
with type strain dsm t references ... sativum analyzed by multilocus sequence analysis systematic and...
https://en.wikipedia.org/wiki/Micromonospora_fulviviridis
*  Micromonospora chokoriensis
surfaces its type strain is t jcm t mtcc t ... sativum analyzed by multilocus sequence analysis systematic and...
https://en.wikipedia.org/wiki/Micromonospora_chokoriensis
*  Bradyrhizobium betae
endophytic the type strain is pl hg t lmg t cec t ... strains based on multilocus sequence analysis of the s rrna gene...
https://en.wikipedia.org/wiki/Bradyrhizobium_betae
*  .. Tag: biology .. Repeated evolution in sticklebacks, and adjacent possibles .. The Impact of a Co
clone-based genome sequencing frequently results in gaps, which ... are toxic to the sequencing host Escherichia coli , including ... of dominant PCR ribotypes. We have undertaken a breadth...
http://molecularist.com/tag/biology
*  A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Tha
from blood. A multilocus sequence typing scheme was developed to ... genotype these pathogenic Leptospira. ... Additional typing was performed for Leptospira isolated...
http://opus.bath.ac.uk/12432/
*  Products - GS FLX+ System : 454 Life Sciences, a Roche Company
... GS FLX+ System. GS FLX+ System. GS FLX+ System Now delivering sequencing reads up to 1,000 bp in length. Available as an on-site instrument upgrade or new instrument, the GS FLX+ System is designed for use with both the new long-read Sequencing Kit XL+ and existing Sequencing Kit XLR70. GS FLX+ System Sequencing Kit. GS FLX Titanium XLR70 Read Length Up to 1,000 bp Up to 600 bp. Mode Read Length 700 bp 450 bp. Throughput Profile - 85% of total bases from reads >500 bp - 45% of total bases from reads >700 bp - 85% of total bases from reads > 300 bp - 20% of total bases from reads > 500 bp. Computing GS FLX+ Computing Station available. monitor 82.5 cm 32.5” H. Lower assembly 75.2 cm × 90.8 cm × 92.7 cm 29.62” × 35.75” × 36.5” W × D × H. GS FLX Titanium Rapid Library MID Adaptors 1 Kit for 96 library preparations 05619211001. GS FLX Titanium Library Paired End Adaptors 1 kit for 10 assays 05463343001. GS FLX Titanium General Library Preparation Kit 1 kit for 10 library preparations 05233747001. GS FLX Tita...
http://my454.com/products/gs-flx-system/index.asp
*  ORNL, Yale Team Advances Virtual Nanopore Method with an Eye to Third-Gen Sequencing System | Genome
ORNL, Yale Team Advances Virtual Nanopore Method with an Eye to Third-Gen Sequencing System. GenomeWeb. Skip to main content. RSS Feeds Twitter LinkedIn. Log in Join now. Business Policy. Business News Research Funding Policy Legislation Regulatory News Reimbursement. Technology. Microarrays Multiplexing PCR Informatics Sequencing Technology Mass Spec Sample Prep Gene Silencing/Gene Editing. Research. Genetic Research Gene Expression Research Epigenetics Research Proteomics Protein Research Cell Biology Research. Clinical. Molecular Diagnostics Companion Diagnostics Biomarker Discovery Validation Drug Discovery Development Clinical Sequencing Clinical Proteomics. Disease Areas. Cancer Infectious Disease Cardiovascular Disease Neurological Psychological Disease Metabolic Disease Autoimmune Disease Inherited Disease Reproductive Health. Applied Markets. Resources. Webinars White Papers The Scan Career Blog Job Listings IP Update New Products People in the News Conferences Events. Main menu. Enter your keywords....
https://genomeweb.com/sequencing/ornl-yale-team-advances-virtual-nanopore-method-eye-third-gen-sequencing-system?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed: genomeweb/insequence (In Sequence)
*  DNA sequencer
These include the 454, SOLiD and Illumina DNA sequencing platforms. 5 More recent, third-generation DNA sequencers such as SMRT and Oxford Nanopore measure the addition of nucleotides to a single DNA molecule in real time. The data may also contain errors, caused by limitations in the DNA sequencing technique or by errors during PCR amplification. Life Technologies. In 2005, 454 Life Sciences released the 454 sequencer, followed by Solexa Genome Analyzer and SOLiD Supported Oligo Ligation Detection by Agencourt in 2006. Applied Biosystems acquired Agencourt in 2006, and in 2007, Roche bought 454 Life Sciences, while Illumina purchased Solexa. More recently, a third generation of DNA sequencers was introduced. Oxford Nanopore Technologies is another company developing third-generation sequencers data using electronic systems based on nanopore sensing technologies. Roche currently manufactures two systems based on their pyrosequencing technology: the GS FLX+ and the GS Junior System. A predecessor to GS FLX+, t...
https://en.wikipedia.org/wiki/DNA_sequencer
*  Genome Sequencing Center offers state-of-the-art sequencing services
... Skip to main navigation University Home MyUCSC People Calendars A-Z Index Search. UC Santa Cruz Menu Newscenter. Awards Honors Events Videos Subscribe Contact Us Helpful Links News by topic News archives E-Newsletter Tuesday Newsday RSS feeds Review Magazine Administrative Messages Budget Updates. Home / 2009 / September / Genome Sequencing Center offers... Genome Sequencing Center offers state-of-the-art sequencing services September 08, 2009 By Tim Stephens, Staff Writer 459-2495. Share this story: Twitter Facebook Google+ LinkedIn Reddit. The UCSC Genome Sequencing Center features state-of-the-art equipment, including the GS FLX Titanium Series sequencing platform from 454 Life Sciences and the SOLiD sequencing platform from Applied Biosystems. Applications include whole-genome and targeted sequencing; resequencing; RNA sequencing; micro-RNA and small-RNA sequencing; chromatin immunoprecipitation ChIP sequencing to identify binding sites of DNA-associated proteins ; and metagenomics also called enviro...
http://news.ucsc.edu/2009/09/3177.html
*  University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology | GEN News
University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Transitioning from Traditional Assay Formats to HTRF Technology. FDA Nominee Driven by "Data, Data, Data". Jobs Report: Data, Business, and Regulatory Savvy Driving New Hiring. Market & Tech An... Transitioning from Traditional Assay Formats to HTRF Technology Sensitivity of Fluorescence Coupled to Low... GEN News Highlights More » Aug 3, 2011 University of Oxford to Sequence Whole Genomes of 500 People Using Illumina Technology Page 1 of 1 Clinicians and geneticists at the University of Oxford have decided to use Illumina s HiSeq 2000 systems to sequence the whole genomes of 500 individuals afflicted with life-threatening diseases. "Our collaboration with Illumina, studying over a hundred different diseases, will allow us to explore the value of wh...
http://genengnews.com/gen-news-highlights/university-of-oxford-to-sequence-whole-genomes-of-500-people-using-illumina-technology/81245503/?kwrd=University of Oxford
*  Unanswered 'dna-sequencing' Questions - Biology Stack Exchange
Unanswered 'dna-sequencing' Questions - Biology Stack Exchange. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. 0 votes. 18 views. microbiology bacteriology dna-sequencing asked yesterday. 0 votes. 27 views. genetics dna-sequencing asked Sep 13 at 11:31. 1 0 votes. 36 views. dna-sequencing virology asked Sep 10 at 21:02. 4 0 votes. 30 views. What do Illumina HiSeq/MiSeq paired end reads look like. dna-sequencing sequence-analysis asked Sep 9 at 17:14. 0 votes. 28 views. Primer Design with Primer-BLAST over specific site I am trying to design primers using Primer-BLAST such that the forward primer spans a specific base pair site. dna-sequencing primer asked Jul 20 at 21:16. 5 votes. 0 answers. 117 views. molecular-genetics dna-sequencing genomics asked Nov 27 '12 at 16:38. 4 votes. 0 answers. 43 views. bioinformatics dna-sequencing sequence-assembly asked Apr 20 at 18:46. 3 3 votes. 0 answers. 27 views. dna dna-sequencing asked Sep 19 at 14...
http://biology.stackexchange.com/questions/tagged/dna-sequencing?sort=unanswered&pagesize=30
*  Re: Log sequence error on db recover
... To : tonye@billy.demon.nl, openldap-software@OpenLDAP.org Subject : Re: Log sequence error on db recover From : Manoj Lal. mlal1974@hotmail.com. Hi Tonni, Yes the db* files are intact after the reboot, but the log files and the bdb files are corrupted. I guess I will have to ensure existence of an ldif to fall back on in case of failure. From: Tony Earnshaw tonye@billy.demon.nl To: openldap-software@OpenLDAP.org Subject: Re: Log sequence error on db recover Date: Sat, 07 Feb 2004 10:46:47 +0100 fre, 06.02.2004 kl. 23.44 skrev Manoj Lal: Try mv'ing the log to an invalid name and running 'db recover -c' again. But bdb files don't get generated. # db recover -c -v db recover: Finding last valid log LSN: file: 4 offset 159583 db recover: Recovery starting from db recover: Recovery complete at Sat Jan 24 07:55:07 2004 db recover: Maximum transaction ID 8000016e Recovery checkpoint db recover: Recovery complete at Sat Jan 24 07:55:07 2004 db recover: Maximum transaction id 80000000 Recovery checkpoint # echo...
http://openldap.org/lists/openldap-software/200402/msg00262.html
*  Nucleotide (Weighted) Links for PubMed (Select 16959970) - Nucleotide - NCBI
Nucleotide Weighted Links for PubMed Select 16959970 - Nucleotide - NCBI. NCBI. DNA RNA. BLAST Basic Local Alignment Search Tool. GenBank. All DNA RNA Resources... BLAST Basic Local Alignment Search Tool. Conserved Domain Search Service CD Search. Search database All Databases Assembly BioProject BioSample BioSystems Books ClinVar Clone Conserved Domains dbGaP dbVar Epigenomics EST Gene Genome GEO DataSets GEO Profiles GSS GTR HomoloGene MedGen MeSH NCBI Web Site NLM Catalog Nucleotide OMIM PMC PopSet Probe Protein Protein Clusters PubChem BioAssay PubChem Compound PubChem Substance PubMed PubMed Health SNP SRA Structure Taxonomy ToolKit ToolKitAll ToolKitBook UniGene Search term. Format Summary GenBank GenBank full FASTA ASN.1 XML INSDSeq XML TinySeq XML Feature Table Accession List GI List. Sort by Default order Accession Date Modified Date Released Organism Name Taxonomy ID. Escherichia coli KRX genome assembly KRX, contig KRX contig52, whole genome shotgun sequence. Escherichia coli KRX genome assembly KR...
http://ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore_weighted&from_uid=16959970
*  Sequencing technologies - the next generation (With NOTES) - ResearchGate
Nature Reviews Genetics. applications of next-generation sequencing REVIEWS NATuRe RevIewS. Seq-based methods Assays that use next-generation sequencing technologies. next-generation sequencing technologies Sequencing technologies include a number of methods that are grouped broadly as template preparation, sequencing and imaging, and data analysis. There are two methods used in preparing templates for NGS reactions: clonally amplified templates origi- nating from single DNA molecules, and single DNA- molecule templates. Modified nucleotides used in next-generation sequencing methods At the core of most next-generation sequencing NGS methods is the use of dye-labelled modified nucleotides. LI-COR Biosciences c Real-time nucleotides b 3′-unblocked reversible terminators a 3′-blocked reversible terminators HN Illumina/Solexa HN Ju et al. The one-colour images highlight the sequencing data from two single-molecule templates. In the first SBL cycle, the A1 primer was annealed to the template, followed by the hybr...
http://researchgate.net/publication/40483884_Sequencing_technologies_-_the_next_generation_(With_NOTES)
*  .. IDT to Provide Custom Primers for Sequencing on 454’s GS FLX and GS Junior P
. Send to printer. GEN News Highlights : Oct 5, 2010. IDT to Provide Custom Primers for Sequencing on 454’s GS FLX and GS Junior Platforms. Firm offers FusionPrimers and Rapid Libary MID Adaptor Oligos for amplicon or shotgun sequencing. Custom oligos firm Integrated DNA Technologies IDT signed an exclusive deal with the Roche company, 454 Life Sciences, for the provision of custom primers for GS FLX Titanium Chemistry used on the latter s GS FLX and GS Junior systems. The products will be available as FusionPrimers or Rapid Library MID Molecular Identification Adaptor Oligos for amplicon or shotgun sequencing. IDT says the GS FLX Titanium FusionPrimers have a 25 base-pair fixed sequence at the 5 end and are designed to enable sequencing of longer amplicons and increased reads per run. An MID sequence for the FusionPrimers is optional and acts as a molecular barcode to enable libraries to be multiplex and identified. The GS FLX Titanium RapidLibrary MID Adaptor Oligos are deisgned for shotgun sequencing and i...
http://genengnews.com/keywordsandtools/print/4/20676/
*  ARS | Publication request: High Throughput Sequence Analysis for Disease Resistance in Maize ARS : R
Publication request: High Throughput Sequence Analysis for Disease Resistance in Maize ARS : Research. Title: High Throughput Sequence Analysis for Disease Resistance in Maize Authors. High Throughput Sequence Analysis for Disease Resistance in Maize. Raw sequence reads were 26bp and 36bp for maize and Aspergillus, respectively. Most reads contained portions of the Solexa gene expression adapter sequences, which were removed before performing alignment, resulting in an average read length of about 18bp for both maize and Aspergillus data. After filtering, Phred quality was analyzed and determined to be on average 31 for maize and 28 for Aspergillus sequences. Initial mappings on maize produced an average of 98% of sequences mapping to a genomic location with up to two mismatches allowed, with about 57% mapping uniquely within the best stratum. Aspergillus sequences fared better with 91% of sequences mapping and almost 73% mapping to a unique genomic location. An overview of possible further computational appr...
http://ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=251303
*  DNA sequencing
The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species. Following the development of fluorescence -based sequencing methods with a DNA sequencer,. Next-generation sequencing methods. Illumina Solexa sequencing. Methods in development Nanopore DNA sequencing. However, there are many other bases that may be present in a molecule. By 2001, shotgun sequencing methods had been used to produce a draft sequence of the human genome. The DNA sample preparation and random surface-PCR arraying methods described in this patent, coupled to Roger Tsien et al.'s "base-by-base" sequencing method, is now implemented in Illumina 's Hi-Seq genome sequencers. The term "'de novo' sequencing" specifically refers to methods used to determine the sequence of DNA with no previously known sequen...
https://en.wikipedia.org/wiki/DNA_sequencing
*  Sequi-Gen GT System | Life Science Education | Bio-Rad
Sequi-Gen GT System. Interchangeable Sequi-Gen GT IPC sizes. Interchangeable Sequi-Gen GT IPC sizes Four Interchangeable Sequi-Gen GT IPC sizes. In addition to DNA sequencing, this system is useful for a wide array of applications: Differential display Microsatellite analysis DNA fingerprinting and footprinting Single-strand conformation polymorphism SSCP studies Heteroduplex analysis RNase protection assays S1 nuclease mapping Primer extension analysis DNA/protein binding studies gel shift assays Oligonucleotide analysis The patented Sequi-Gen GT nucleic acid sequencing cell makes casting and running sequencing gels easy. Simple, problem-free horizontal gel casting Unique design for heat dissipation maintains uniform temperature and eliminates smile patterns Easy-to-use, lever-operated clamps facilitate rapid assembly and gel casting without tape and grease Modular design includes a universal transparent base for all four Sequi-Gen sizes Convenient drain port in the sealed upper buffer chamber allows safe an...
http://bio-rad.com/en-ca/product/sequi-gen-gt-system?pcp_loc=catprod
*  molecular biology - What is the difference between SOLiD, 454, and Illumina next-gen sequencing? - B
molecular biology - What is the difference between SOLiD, 454, and Illumina next-gen sequencing. - Biology Stack Exchange. Biology. Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. What is the difference between SOLiD, 454, and Illumina next-gen sequencing. up vote 9 down vote favorite 3. molecular-biology genomics dna-sequencing share. improve this question. edited Feb 5 '13 at 20:12. asked Feb 5 '13 at 19:55. MattDMo 6,151. GWW Feb 5 '13 at 20:07. MattDMo Feb 5 '13 at 20:09. 2 Answers 2 active oldest votes. The fragments are mixed with agarose beads, which carry adapters complementary to the library adapters, and thus each bead is associated with a unique DNA fragment. Although sequencing on 454 platform is more expensive than sequencing on Illumina platform 40USD per Mega base versus 2USD per Mega base,...
http://biology.stackexchange.com/questions/7075/what-is-the-difference-between-solid-454-and-illumina-next-gen-sequencing
*  Finished sequence of human chromosome 20
finished sequence of human chromosome home about topics finished sequence of human chromosome by edward r winstead december researchers at the wellcome trust sanger institute in cambridge u k have published the dna sequence of human chromosome they identified genes including novel genes the annotation was done through comparative analyses using the protein sets of sequenced organisms partial human gene sequences or ests and mouse and pufferfish genomic sequences the mouse and pufferfish data were not available when a draft of this chromosome was published in february chromosome is about million base pairs long and the new sequence has four gaps or regions that were extremely difficult to analyze the third smallest human chromosome contains genes involved in creutzfeldt jakob disease and severe combined immunodeficiency it is the third finished chromosome published by members of the international consortium known as...
http://genomenewsnetwork.org/articles/12_01/Human_chromosome_20.shtml
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Liquid Handling. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/sequencing-instruments-and-kits/page-2/?catID=4740&viewAll=1&pageSize=25&u=5A4CAC50-53DC-41D4-9F65-8FDA5DFB81D0
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/sequencing-instruments-and-kits/page-2/?catID=4740&viewAll=1&pageSize=25&u=487214CD-D567-4FF9-9B2B-B297C8902196
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits Laboratory Product Dir
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. Microscopy. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing Sequencing Instruments and Kits 48 ChiP Sequencing 22 de novo Sequencing 2 DNA Sequencing 5 Show more...test. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/sequencing-instruments-and-kits/page-2/?catID=4740&viewAll=1&pageSize=25&u=E697E4BA-C291-482C-8DC9-56DCD1A3D415
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=5A4CAC50-53DC-41D4-9F65-8FDA5DFB81D0
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Products Reviews. Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=DC157CD8-B624-4748-8B4A-5FE3931221CB
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=356629B4-AA02-4250-8BCD-DCED93DB8A9D
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
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*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=C91A6BAF-724C-4F1B-B2FB-E534582F609F
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=CA448E1D-39C4-4A9B-959D-BF8A347807C0
*  Next Generation Sequencing (Life Sciences) > Sequencing Instruments and Kits > ChiP Sequencing Labo
Read more Articles Videos. Latest Editorial Buying Guides Mass Spectrometry. DNA and RNA Purification. < Products & Reviews < Life Sciences < Next Generation Sequencing < Sequencing Instruments and Kits ChiP Sequencing 22. Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro-A 96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-... Compare or Request Pricing for Selected Product s Enzymatic Chromatrap® Standard Pro-A 96 Well Microplate Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ChIP technique It is essentially 96 separate Chromatrap® spin-c... Compare or Request Pricing for Selected Product s Chromatrap® Standard Pro G-96 Well Microplate Sonication Shearing Kit Porvair Sciences Ltd The Chromatrap® high throughput ChIP microplate is based on the novel spin-column ...
http://selectscience.net/next-generation-sequencing-(life-sciences)/product-directory/chip-sequencing/?catID=4741&u=ED141727-86D9-4935-B43B-8E989BC5F704
*  Patent US6110683 - Automated DNA Sequencer loading dye which contains a lane tracking aid - Google P
A marker to identify individual lanes on an electrophoresis gel run on an automated DNA sequencer, comprising the loading buffer used in the automated DNA sequencer; a DNA sequencing sample; and a lane-identifying marker DNA fragment labeled with a fluorophore, wherein the fluorophore is detectable by the DNA sequencer. A marker to identify individual lanes on an electrophoresis gel run on an automated DNA sequencer, comprising the loading buffer used in the automated DNA sequencer; a DNA sequencing sample; and a lane-identifying marker DNA fragment labeled with a fluorophore, wherein the fluorophore is detectable by the DNA sequencer, and wherein the length of the DNA fragment is selected such that the labeled DNA fragment will enter the gel to a migration distance slightly longer than the migration distance of the first set of sequencing data from the sequencing reaction, such that inclusion of the marker DNA does not obstruct, interfere, or obscure any of the sequencing data. A marker to identify individua...
http://google.ca/patents/US6110683
*  Why are there N's after Sanger sequencing? - Biology Stack Exchange
Why are there N's after Sanger sequencing. - Biology Stack Exchange. Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Tour Start here for a quick overview of the site. Help Center Detailed answers to any questions you might have. Biology Questions. Users. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Why are there N's after Sanger sequencing. After sending a DNA sample for sequencing, the resulting sequence had N's in the beginning and end of the sequence. dna-sequencing share. improve this question. add a comment. 2 Answers 2 active oldest votes. up vote 9 down vote. Typically sanger sequencing will run into a few errors. Sometimes the traces will overlap as below in red and the computer will call N. improve this answer. I'll add on to this answer. A Sanger sequence is really only good for at best 800-900 bp. add a comment. up vote 4 down vote. N bases at end of the sequence simply could ...
http://biology.stackexchange.com/questions/1830/why-are-there-ns-after-sanger-sequencing?answertab=votes
*  Medical Xpress - genome sequencing(... continued page 4)
Medical Xpress - genome sequencing ... continued page 4. Home genome sequencing. News tagged with genome sequencing. sort by:. Date. 6 hours. 12 hours. 1 day. 3 days. all. Rank. Last day. 1 week. 1 month. all. LiveRank. Last day. 1 week. 1 month. all. Popular. Last day. 1 week. 1 month. all. Related topics: dna sequences · genome. Cancer. Researchers confirm whole-genome sequencing can successfully identify cancer-related mutations. UT Southwestern Medical Center cancer researchers have demonstrated that whole-genome sequencing can be used to identify patients' risk for hereditary cancer, which can potentially lead to improvements in cancer prevention, ... Dec 23, 2014 1 0. Genetics. A computer platform can pinpoint the genes behind rare diseases that have eluded diagnosis. A computer program that cross-references disease symptoms with DNA sequencing data can detect the faulty genes responsible for rare disorders with greater accuracy than other methods. Developed by scientists at A*STAR, the ... Dec 17, 2014...
http://medicalxpress.com/tags/genome sequencing/page4.html
*  DNA sanger sequencing - Best Price Segment Microsynth AG -
... DNA/RNA Oligo Synthesis. DNA/RNA Analysis & Sequencing. DNA Oligonucleotides Best Price Segment. DNA Oligos — Volume Orders DNA Oligos — Subscription Orders. Standard Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes. Premium Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes Large-Scale DNA Oligos. RNA Oligonucleotides Best Price Segment. RNA Oligos - Volume Orders siRNA - Volume Orders. Standard Segment. Unmodified RNA Oligos Modified RNA Oligos siRNA. Premium Segment. RNA Oligos Large-Scale RNA Oligos. Technical Information. Sanger Sequencing Best Price Segment. Barcode Economy Run Single Tube Barcode HT Plate Service HT Plate Service - Volume Orders. Standard Segment. Economy Run Single Tube HT Plate Service HT Plate Service - Ready-to-Load. Premium Segment. Technical Information. Technical Information. Hints and Tips. DNA/RNA Analysis DNA/RNA Isolation. Project-based Analysis Services Customized qPCR Assays qPCR Assays for Food Testing. Fragment Length Analysis Human Cell Li...
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*  DNA sanger sequencing - Best Price Segment Microsynth AG -
... DNA/RNA Oligo Synthesis. DNA/RNA Analysis & Sequencing. DNA Oligonucleotides Best Price Segment. DNA Oligos — Volume Orders DNA Oligos — Subscription Orders. Standard Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes. Premium Segment. Unmodified DNA Oligos Modified DNA Oligos qPCR Probes Large-Scale DNA Oligos. RNA Oligonucleotides Best Price Segment. RNA Oligos - Volume Orders siRNA - Volume Orders. Standard Segment. Unmodified RNA Oligos Modified RNA Oligos siRNA. Premium Segment. RNA Oligos Large-Scale RNA Oligos. Technical Information. Sanger Sequencing Best Price Segment. Barcode Economy Run Single Tube Barcode HT Plate Service HT Plate Service - Volume Orders. Standard Segment. Economy Run Single Tube HT Plate Service HT Plate Service - Ready-to-Load. Premium Segment. Technical Information. Technical Information. Hints and Tips. DNA/RNA Analysis DNA/RNA Isolation. Project-based Analysis Services Customized qPCR Assays qPCR Assays for Food Testing. Fragment Length Analysis Human Cell Li...
http://microsynth.ch/de/10174/DNA-Sanger-Sequencing.html
*  TraceTuner download | SourceForge.net
TraceTuner download. SourceForge.net. SourceForge. Browse Enterprise Blog Jobs Deals Help. Log In or Join. Solution Centers Go Parallel Performance Central. Resources Newsletters. Home. Browse. Science Engineering. Bio-Informatics TraceTuner. TraceTuner DNA sequencing quality values, base calling and trace processing Brought to you by: gdenisov. Summary. Files. Reviews. Support. Wiki. Code. Tickets ▾. Feature Requests. Bugs. 5.0 Stars 3. 4 Downloads This Week. Last Update: 2013-12-14. Download tracetuner 3.0.6beta.tar.bz2. Browse All Files. Windows BSD Linux. Screenshots. Description Tracetuner is a tool for base and quality calling of trace files from DNA sequencing instruments. Originally developed by Paracel, a Celera Business, this code base was released as open source in 2006. TraceTuner was used by Celera to call 30+ million reads from both Drosophila and human genome sequencing projects. In 2000, Applied Biosystems bundled TraceTuner with ABI3700 Genome Analyzers and shipped it to the customers of thes...
http://sourceforge.net/projects/tracetuner/?sort=date&source=recommended&stars=1
*  Whitepapers for analytics, lab technology, diagnostics, biotechnology
Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System. Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System. Metagenomics Analysis Using the Genome Sequencer FLX System. 05-Sep-2007 The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small R more. Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology. Real-Time PCR Quality Control for Gene Expression. Profiling Using the LightCycler® 480 System 28-Aug-2007 Quantitative real-time PCR qPCR has become the de facto standard for nucleic acid quantification. New Application for the LightCycler® 480 System: Gene Scanning by High-Resolution Melting. 28-Aug-2007 High-resolution melting HRM is a novel, homogeneous, post-PCR method, enabling genomi...
http://analytica-world.com/en/whitepapers/p3/
*  Phred base calling
'Phred base-calling' is a computer program for identifying a base nucleobase sequence from a fluorescence "trace" data generated by an automated DNA sequencer that uses electrophoresis and 4-fluorescent dye method. 1998 : Base-calling of automated sequencer traces using phred. 1998 : Base-calling of automated sequencer traces using phred. PMID 9521922 When originally developed, Phred produced significantly fewer errors in the data sets examined than other methods, averaging 40–50% fewer errors. The electrophoresis run is monitored by a CCD on the DNA sequencer and this produces a time "trace" data or " chromatogram " of the fluorescent "peaks" that passed the CCD point. Examining the fluorescence peaks in the trace data, we can determine the order of individual bases nucleobase in the DNA. It is currently the most widely used base-calling software program by both academic and commercial DNA sequencing laboratories because of its high base calling accuracy. Phred uses a four-phase procedure as outlined by Ewin...
https://en.wikipedia.org/wiki/Phred_base_calling
*  [linux-dvb] Re: High error rates when running Athcool
Re: High error rates when running Athcool. Re: High error rates when running Athcool Uwe Maier umaier at gmx.de. Tue Feb 7 13:55:29 CET 2006. Previous message: Re: High error rates when running Athcool Next message: Re: High error rates when running Athcool Messages sorted by:. For me it indeed makes a difference: 66W vs. 105W and 40W fewer power consumption means fewer 40W to cool - less noise. On higher load more than 3 recordings at the same time I get artefacts lost frames so I switch it off when a recording starts. Regards, uwe Rudo Thomas wrote: Hi. To keep the power consumption of the machine down I use 'Athcool'. This utility tweaks registers on the Northbridge chip so that when the CPU is idle it goes into a 'deep sleep' mode where is uses very little power. This is the output of 'dvbtune -m' on C5 with Athcool running: Signal=14135, Verror=11891, SNR=40349dB, BlockErrors=99, S|L|C|V|SY. Signal=14135, Verror=13445, SNR=39578dB, BlockErrors=191, S|L|C|V|SY. Signal=14135, Verror=12998, SNR=42662dB, Blo...
http://linuxtv.org/pipermail/linux-dvb/2006-February/008106.html
*  ChiRP-Seq
... 'ChiRP-Seq' Chromatin Isolation by RNA purification is a high-throughput sequencing method to discover regions of the genome which are bound by a specific RNA or a by a ribonucleoprotein containing the RNA of interest. Recent studies have shown that a significant proportion of some genomes including mouse and human genomes synthesize RNA that apparently do not code for proteins. Various genomic methods are being developed to map the functional association of these novel RNA to distinct regions of the genome to gain a better understanding of their function. ChiRP-Seq is one of these new methods which uses the massively parallel sequencing capability of 2nd generation sequencers to catalog the binding sites of these novel RNA molecules on a genome. 'Overview of ChiRP-Seq method:' Tens of oligonucleotide probes are designed to be complementary to the RNA of interest. Cells are cross-linked by UV or formalin and nuclei are isolated from these treated cells. Complexes containing biotin-probe + RNA of interest...
https://en.wikipedia.org/wiki/ChiRP-Seq
*  What does "resequence" mean? (NONE)
What does resequence mean. NONE. What does resequence mean. NONE Clemens Suter-Crazzolara c.suter-crazzolara at dkfz-heidelberg.de. Wed Mar 31 04:27:55 EST 1999. Previous message: What does resequence mean. NONE. Next message: Unwanted fluoresence in liposome-mediated transfection Messages sorted by:. Yang Sheng wrote: Thank you. if for some reason the sequencing reaction was satisfactory double bands, smears, no incorporation of label etc. etc. etc. you may want to decide to resequence yout fragment. clemens suter-crazzolara. Previous message: What does resequence mean. NONE. Next message: Unwanted fluoresence in liposome-mediated transfection Messages sorted by:. More information about the Methods mailing list....
http://bio.net/bionet/mm/methods/1999-March/074679.html
*  BioSolutions: DNA Sequencers
... BioSolutions. Online repository of biological information which aims to create a knowledge base for students by the provision of animations and lectures. DNA Sequencers. DNA sequencers have become more important due to large genomics projects and the need to increase productivity. Modern automated DNA sequencing instruments called DNA sequencers are able to sequence as many as 384 fluoresecently labeled samples in a batch run and perform as many as 24 runs a day. These perform only the size separation and peak reading; the actual sequencing reaction s, cleanup and resuspension in a suitable buffer must be performed separately. The magnitude of the fluorescent signal is related to the number of strands of DNA that are in the reaction. If the initial amount of DNA is small, the signals will be weak. However, the properties of PCR allow one to increase the signal by increasing the number of cycles in the PCR program. A simple DNA sequencer will have one or more lasers that emit at a wavelength that is absor...
http://bioisolutions.blogspot.com/2008/03/dna-sequencers.html
*  Whole Genome Sequencing of Wild Rice Reveals the Mechanisms Underlying Oryza Genome Evolution
... . BGI News More. Complete Genomics Announces UK Epilepsy Society s Acquisition of Its Revolocity Supersequencer. BGI has received accreditation from the College of American Pathologists CAP. BGI Poised to Launch Its Desktop Sequencer BGISEQ-500 This October. Complete Genomics Previews Revolocity Sequencing System at European Human Genetics Conference 2015. Academic and Education More. Ancient Alga Knew How to Survive on Land before It Left Water Evolved into First Plant. BGI Presents the First Gene Catalog of Mouse Gut Microbiome. Lanosterol revealed clues for cataract prevention and treatment. Metagenome-wide association study extended to the oral microbiome and uncovered markers for rheumatoid arthritis. Research Collaboration More. Huawei and BGI Sign Joint Development Agreement on Big Data Storage Systems for Genetic Research at HCC2015. BGI Health Forms Partnership with University of Mayor in South America. BGI Health Joins Hand with Star Metropolis Clinical Laboratories to Provide Genetic Testing S...
http://genomics.cn/en/news/show_news?nid=99454
*  View Profile: Phred - Pet Talk
... Today's Dog of the Day. Today's Cat of the Day. Today's Pet of the Day. New Posts. Today's Posts. Today's Posts. Phred. PetTalk members may login in the top right corner or new users may register in order to post. Phred. Find latest posts. Find latest started threads. View Blog Entries. Mini Statistics Join Date 10-19-2000. Last Activity 05-20-2010 02:17 PM. More 3 Friends captain. AmberLee. krazyaboutkatz. Tab Content. Phred's Activity. Visitor Messages. Friends. Post Areas. Tab Content. All Phred Friends Photos. New Activity. 09-07-2015, 07:32 PM krazyaboutkatz replied to a thread Bichon Frise' addition - Please meet the puppy. 2 new pics page 2 in Dog General Congrats!!!:D Welcome to your new loving and caring forever home handsome Frankie.: I must've missed your latest cat addition because I only see 7... 31 replies. 1242 view s. 09-07-2015, 07:21 PM krazyaboutkatz replied to a thread Sky Is Gone in Cat Memorial Thanks everyone. 26 replies. 788 view s. 09-06-2015, 12:05 AM krazyaboutkatz started a th...
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*  Taking Sequencing Forward - Wellcome Trust Sanger Institute
... Jump to navigation. Jump to content. Search for. A A A A. Home. Research. Scientific resources. Work study. About us. What we do. History. How we work. People. Press. Public engagement. Campus. Contact 14th January 2008 Taking Sequencing Forward New Team to Lead DNA Sequencing at the Sanger Institute Sequencing is of fundamental importance to the science of the Sanger Institute now and in the future. The new team will enhance the Institute's global role in delivery of valuable sequence data and analysis and ensure that those efforts meet the needs of the wider community. The Wellcome Trust Sanger Institute has recently made a major investment in new DNA sequencing technology which will increase production 60-fold. The Institute today announces the appointment of a new senior management team to lead the Institute's efforts to generate DNA sequence data of value to its core programmes and to the wider biomedical research community. Professor Julian Parkhill takes on a new role as Director of Sequencing...
http://sanger.ac.uk/about/press/2008/080114.html
*  IntelligentBioSystems.com
intelligentbiosystems com about ibs technology applications career center contact us fail the browser should render some flash content not this the new concept of benchtop sequencing ibs has developed a new type of benchtop sequencing system that is capable of performing up to independent sequencing runs in parallel and allows random loading of the flow cells at any time during a run the new system is just about to enter early beta testing the ibs team intelligent bio systems is a group of innovative and experienced chemists molecular biologists and engineers capable of bringing breakthrough technologies to market quickly click here for a list of open positions at the company about us intelligent bio systems was founded in by drs steven j gordon and jingyue ju to commercialize an advanced dna sequencing system dr gordon previously founded intelligent automation systems which was responsible for a number of key instrumentation solutions which helped to complete the human genome project dr ju is one of the key ...
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*  DNASTAR - Training Videos Flash Version | DNASTAR
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*  JGI GOLD | Project
JGI GOLD. Project. JGI HOME LOG IN. Home. Search. Quick Search. Advanced Search. Metadata Search. Distribution Graphs. Biogeographical Metadata. Google Map. Google Earth. Statistics. References. Team. Help. News. Studies. 22,233. Biosamples. 69,152. Sequencing Projects. 69,468. Analysis Projects. 57,279. Project Information. Sequencing Information. Organism Information. Organism Metadata. PROJECT NAME GOLD Project ID. Gp0001259. Project Name. Hoeflea phototrophica DFL-43. Other Names. Legacy ER Project ID. 11154. Legacy GOLD ID. Gi01415. NCBI BioProject Name Hoeflea phototrophica DFL-43. NCBI BioProject ID. 19311. NCBI BioProject Accession. PRJNA19311. NCBI Locus Tag. HPDFL. NCBI BioSample Accession. PI. Added By Nikos Kyrpides on 2007-11-27. Last Modified By Auto script update processes on 2014-10-31. Project Comments. PROJECT TYPE Specimen. Organism. Nucleic Acid. DNA. Sequencing Strategy. Whole Genome Sequencing. PROJECT INFORMATION Project Status. Permanent Draft. Project Relevance. Environmental Marine M...
https://gold.jgi.doe.gov/project?id=Gp0001259
*  Newest 'bioinformatics' Questions - Page 2 - Biology Stack Exchange
Newest 'bioinformatics' Questions - Page 2 - Biology Stack Exchange. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Tagged Questions info newest frequent votes active unanswered. 3 votes. 1 answer. 31 views. 0 votes. 0 answers. 51 views. 4 1 vote. 1 answer. 64 views. bioinformatics dna-sequencing pcr sequence-analysis primer asked Jul 1 at 13:56. 3 votes. 2 answers. 56 views. 1 vote. 2 answers. 73 views. bioinformatics asked Jun 24 at 2:26. 7 -1 votes. 1 answer. 51 views. -1 votes. 3 answers. 47 views. bioinformatics proteins dna-sequencing asked Jun 21 at 7:16. 2 1 vote. 1 answer. 196 views. bioinformatics asked Jun 20 at 12:05. genetics neuroscience bioinformatics book-recommendation asked Jun 16 at 15:11. evolution bioinformatics phylogenetics sequence-analysis asked Jun 10 at 12:45. genetics bioinformatics gene-regulation computational-model asked Jun 6 at 21:38. molecular-biology bioinformatics pcr primer asked Jun 4 at 5:45. bioinformatics molecu...
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*  Genotyping and Gene Expression - The Online Scientific Community - News
... Scientific Community. Predictive Biosciences Announces Next-Generation Sequencing Assay for p53. Predictive Biosciences announced it has developed a new next-generation sequencing NGS assay for the TP53 gene encoding the p53 protein that will enhance the clinical performance of its bladder cancer assay. The new CertNDx TM Bladder Cancer Assay incorporating TP53 is expected to be commercially available in early 2013. Predictive's next-generation sequencing assay for TP53 can detect 133 different mutations previously identified in bladder cancers. A research study conducted on 200 control samples and 36 cancers, incorporating TP53 sequence analysis into the existing assay, demonstrated an improvement over the previously published test by increasing sensitivity across bladder cancer pathways while retaining very high specificity and high Positive Predictive Value PPV. The CertNDx test now fully incorporates the different pathways of bladder cancer. "In addition to incorporating p53 into the existing CertNDx...
http://technologynetworks.com/Genotyping/news.aspx?id=146947
*  .. Whole Genome Sequencing in Diagnostics? .. Share this: .. Like this:
whole genome sequencing in diagnostics by dr bertalan meskã on september using whole genome sequencing in diagnostics has been an issue for years and as the cost of sequencing is rapidly declining it seems it can pave the way for personalized medicine a new research published in genome biology evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors just proves this point adenocarcinomas of the tongue are rare and represent the minority to of salivary gland tumors affecting the tongue we investigated the utility of massively parallel sequencing to characterize an adenocarcinoma of the tongue before and after treatment we conclude that complete genomic characterization of a rare tumor has the potential to aid in clinical decision making and identifying therapeutic approaches where no established treatment protocols exist these results also provide direct in vivo genomic evidence for mutational evolution within a tumor under drug selection and potential mechanisms of drug resistance...
http://scienceroll.com/2010/09/24/whole-genome-sequencing-in-diagnostics/?like=1&_wpnonce=469fbe03a0
*  .. Whole Genome Sequencing in Diagnostics? .. Share this: .. Like this:
Whole Genome Sequencing in Diagnostics. by Dr. Bertalan Meskó on September 24, 2010. Using whole genome sequencing in diagnostics has been an issue for years, and as the cost of sequencing is rapidly declining, it seems it can pave the way for personalized medicine. A new research published in Genome Biology, Evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors, just proves this point:. Adenocarcinomas of the tongue are rare and represent the minority 20 to 25% of salivary gland tumors affecting the tongue. We investigated the utility of massively parallel sequencing to characterize an adenocarcinoma of the tongue, before and after treatment. We conclude that complete genomic characterization of a rare tumor has the potential to aid in clinical decision making and identifying therapeutic approaches where no established treatment protocols exist. These results also provide direct in vivo genomic evidence for mutational evolution within a tumor under drug selection and potentia...
http://scienceroll.com/2010/09/24/whole-genome-sequencing-in-diagnostics/?like=1&_wpnonce=cb13afa090
*  Recent Articles | Whole Genome Sequencing And Neuroscience | The Scientist Magazine®| Page 6
Recent Articles. Whole Genome Sequencing And Neuroscience. The Scientist Magazine. Page 6. The Scientist Sign In or Register. Advertisement. The Scientist. tags: whole genome sequencing x. neuroscience x. The Scientist whole genome sequencing and neuroscience. Most Recent Three Monkey Brains, One Robotic Arm By Jef Akst. July 10, 2015. Researchers network the brains of three monkeys to create a living computer that can steer an image of a robotic arm toward a target. 0 Comments. Gene Therapy Fixes Mouse Hearing By Kerry Grens. July 9, 2015. Expressing a gene for a component of the inner ear s hair cells treated a form of genetic deafness. 0 Comments. Can We Smell A Trillion Odors. By Kerry Grens. July 8, 2015. A reanalysis calls into question a year-old claim that humans can decipher at least 1 trillion different scents. 2 Comments. Capsule Reviews By Bob Grant. July 1, 2015. Stoned, Anxious, The Deeper Genome, and Testosterone 0 Comments. New Human Brain Language Map By Bob Grant. June 26, 2015. Researchers ...
http://the-scientist.com/?articles.list/tagNo/2416,11/tags/whole-genome-sequencing,neuroscience/pageNo/6/
*  King’s Proclamation for Citizen Scientists - Bio-IT World
... News. RELATED STORIES A Worthy Sequel: PacBio's New Sequencing System. King’s Proclamation for Citizen Scientists. We very much hope that something we do in the next 20 years will preclude another million women dying of the disease.”. Back in It was 1994, King, for years the personification of the war against breast cancer, had suffered the scientific equivalent of a death in the family. King had been studying the genetics of breast cancer for two decades, stubbornly believing that the disease must have a hereditary component when most so-called experts rubbished the idea. “BRCA” stands for breast cancer although at the time King joked it signified “Berkeley, California.” The breakthrough had come when King’s group sorted their families by age-of-onset. Seven affected families with early-onset disease provided unequivocal data supporting linkage of a DNA marker on chromosome 17 to breast cancer susceptibility; the signal tailed off in other families, indicating the confounding role of other genetic a...
http://bio-itworld.com/BioIT_Article.aspx?id=120390
*  Project: 2/5-Elucidating the genetic architecture of autism by deep genomic sequencing
... Projects Home Project Detail. 2/5-Elucidating the genetic architecture of autism by deep genomic sequencing. This research will be conducted through a partnership between expert large-scale sequencing centers and a collaborative network of research labs focused on the genetics of autism to utilize novel high-throughput genome sequencing to discover specific genes underlying the significant heritability of autism. Without knowledge of the specific genes and DNA variants that predispose to disease, the basic starting point with which the biological processes of disease might be understood is missing. The project will first support a detailed examination of 1000 genes implicated by previous genetic studies or postulated to be functionally relevant, and later, as the technology continues to advance, through unbiased whole-genome sequencing. By combining large, well-characterized patient samples, experienced DNA sequencing teams and a collaborative, expert analysis and follow-up network, this study will provi...
https://iacc.hhs.gov/apps/portfolio-analysis-web-tool/project?projectId=3665&fy=2010
*  TraceTuner
Files from TraceTuner Tracetuner is a tool for base and quality calling of trace files from DNA sequencing instruments. Originally developed by Paracel, a Celera Business, this code base was released as open source in 2006. TraceTuner was used by Celera to call 30+ million reads from both Drosophila and human genome sequencing projects. In 2000, Applied Biosystems bundled TraceTuner with ABI3700 Genome Analyzers and shipped it to the customers of these capillary electrophoresis sequencers. Later versions of TraceTuner, which support mixed base calling, have been used by the research community, the private biotech sector, and the U.S. government as components of different variant detection, genotyping and forensic software applications (e.g. Applied Biosystems SeqScape, Paracel Genome Assembler, MTexpert, etc.)....
http://sourceforge.net/projects/tracetuner/rss?path=/
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. So PCR is used routinely to amplify DNA before sequencing. The sequencing itself isn’t influenced by this – you use whatever technique is appropriate. However, the PCR may introduce biases into the data because not all DNA fragments amplify equally efficiently. Coming back to your question, PCR isn’t a sequencing method in itself. Furthermore, most sequencing methods actuall...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr/2805
*  bioinformatics - What's the use of DNA sequencing results? - Biology Stack Exchange
- Biology Stack Exchange. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. What's the use of DNA sequencing results. dna bioinformatics dna-sequencing share. add a comment. 3 Answers 3 active oldest votes. up vote 7 down vote. The elephant in the room are of course coding sequences : stretches of DNA, contained in so-called “ genes ” – which code for proteins and other stuff. Once you have found the genes in a sequence which you can trivially translate to amino acid sequences, you can try to form inferences about the function of the proteins a protein’s function is an almost-direct consequence of its sequence. Unfortunately, discovering the function of a protein from its sequence alone isn’t possible but we do know the functions of many proteins. When we now look at a genome and fi...
http://biology.stackexchange.com/questions/1884/whats-the-use-of-dna-sequencing-results/1886
*  Nuclear run-on
About 10 6 Cell nuclei are isolated and incubated with labeled nucleotide s and genes in the process of being transcribed are detected by hybridization of extracted RNA to gene specific probes on a blot. Alternative microarray methods have recently been developed, mainly PolII RIP-chip: RNA immunoprecipitation of RNA polymerase II with phosphorylated C-terminal domain directed antibodies and hybridization on a microarray slide or chip the word chip in the name stems from "ChIP-chip" where a special affymetrix genechip was required. A comparison of methods based on run-on and ChIP-chip has been made in yeast Pelechano et al., 2009. It has to be considered that run-on only detects elongating RNA polymerases whereas ChIP-chip detects all present RNA polymerases, including backtracked ones. thumb|Overview of the Global run on sequencing assay for delineating genes, genome-wide, that are engaged in transcription. Therefore only genes that already have an RNA polymerase will produce labeled transcripts. RNA transcr...
https://en.wikipedia.org/wiki/Nuclear_run-on
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. You either need to extract DNA from a large-ish tissue sample or you need to amplify DNA content from a smaller sample. So PCR is used routinely to amplify DNA before sequencing. Furthermore, most sequencing methods actually include PCR steps as well, since they rely on the generation of of overlapping DNA fragments to be stitched together. add a comment...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr?answertab=votes
*  dna sequencing - ChIP-seq vs ChIP-exo - Biology Stack Exchange
... chat blog. Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. help. Help Center Detailed answers to any questions you might have. Biology Questions. Ask Question. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. ChIP-seq vs ChIP-exo. I'm currently investigating ChIP-seq vs. ChIP-exo for finding binding sites. As far as I can tell, ChIP-exo seems to be better in every way than ChIP-seq... Are there any weaknesses in the ChIP-exo approach. dna-sequencing transcription protein-binding binding-sites chip share. improve this question. asked Jun 6 '12 at 3:07. add a comment. 1 Answer 1 active oldest votes. up vote 4 down vote. ChIP-exo does seem to be the "ChIP-seq killer." I've seen Dr. improve this answer. answered Jun 7 '12 at 19:38. add a comment. Your Answer. Email. Email. Not the answer you're looking for. Browse other questions tagged dna-sequencing transcription protein-binding binding-sites chi...
http://biology.stackexchange.com/questions/2512/chip-seq-vs-chip-exo?answertab=active
*  Sequencing from PCR - Biology Stack Exchange
... Biology. Biology Meta. more stack exchange communities. Stack Exchange. sign up log in tour. Help Center Detailed answers to any questions you might have. Biology Questions. Sign up. Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Sequencing from PCR. up vote 4 down vote favorite. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. dna-sequencing pcr share. improve this question. asked Jul 2 '12 at 0:57. add a comment. 2 Answers 2 active oldest votes. All sequencing methods, be it classical Sanger sequencing or next-generation sequencing or even third generation need a certain amount of DNA to work with. So PCR is used routinely to amplify DNA before sequencing. Furthermore, most sequencing methods actually include PCR steps as well, since they rely on the generation of of overlapping DNA fragments to be stitched together. add a comment. up vote 4 down vote. Yes, I'm actually more intere...
http://biology.stackexchange.com/questions/2804/sequencing-from-pcr
*  GEN News Highlights Related To | GEN
Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Transitioning from Traditional Assay Formats to HTRF Technology. Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints. Transitioning from Traditional Assay Formats to HTRF Technology. Linking Phenotypes and Modes of Action Through High-Content Screen Fingerprints The Use of High-Content Screening as... GEN News Highlights Related to Covaris to Supply Sample-Prep Tool for BGI's Next-Generation Sequencing Projects Human Longevity, Discovery to Offer Clients Sequencing, Analysis for $250 NIH Awards $13M in Grants Toward Identifying Genomic Variants Ye Shall Know Them by Their Microbial Clouds Sequencing Metastatic Cancers Could Lead to Improved Therapies Using Nanopores to Study Real-Time Enzymatic Processes Detecting All Infective Viruses at Once Gene Region That Protects Children from Severe Malaria Identified 1000 Genomes Project Wraps Up at 2,504 ZyGEM Bolsters DNA Extraction Kit Distribution in Asia P...
http://genengnews.com/more/related/gen-news-highlights/4/23660/?page=2
*  [Numpy-discussion] Find contiguous sequences of booleans in arrays
find contiguous sequences of booleans in arrays find contiguous sequences of booleans in arrays charles r harris charlesr harris gmail mon sep cdt previous message segfault on wrong use of indexing next message confusion about min max messages sorted by hi kurdt on kurdt bane kurdt bane gmail com wrote hi to all i ve got an d array of bools and i d like to find the length of the first contiguous sequence of true values starting from position of the array that s equivalent to find the position of the first occurrence of false in the array the problem is trivial but i was wondering what s the best fastest cleanest most pythonesque way to do it in numpy and what if i want to get a list of all the contiguous sequences of true values above a given threshold you can find the start of all runs after the first by in a array dtype bool in s arange len a a in s out array i e the first run is a the second a etc and the runs alternate between true and false chuck next part an html attachment was scrubbed url http project...
https://mail.scipy.org/pipermail/numpy-discussion/2007-September/029250.html
*  SHOTGUN SEQUENCING
... Bruce Roe broe at aardvark.ucs.uoknor.edu. Mon Dec 13 08:30:00 EST 1993. Previous message: SHOTGUN SEQUENCING Next message: Rainbow markers Messages sorted by:. In article 1993Dec7.084144.4413 at crc.ac.uk, mjones at crc.ac.uk Dr. M.D. Jones writes... Hi netters One of the favoured ways of sequencing large DNA fragments is to shotgun clone 'sonicated' DNA into a M13 vector and sequence the clones, and compile the data by computer. In the past I have successfully used this strategy, but the most frustrating steps were getting enough recombinant clones to complete the sequence. This is due to the damage caused by sonicating the DNA, making most of it unclonable. Does anybody out there have a detailed fool-proof 'sonication' protocol. Anybody have any advice/tips/etc on how to get the best out of this procedure. On a similar note, does anybody use 'partial' restriction enzyme digestion to fragment their target DNA. Fitzgerald et al, 1992 Nucleic Acids Res., 20 14, 3753-3762 used the enzyme CviJI recognition...
http://bio.net/bionet/mm/methods/1993-December/009822.html
*  Technique of DNA-sequencing
technique of dna sequencing technique of dna sequencing patrick harenberg ebola at nikocity de fri mar est previous message human genome next message pre registration instructions messages sorted by hi can anyone of you tell me or even send me a paper that exactly describes the technique pcr electrophoresis etc of sequencing dna i e getting the exact base sequence of a viral genome answers please to ebola at nikocity de thanks in advance cu patrick harenberg previous message human genome next message pre registration instructions messages sorted by more information about the biochrom mailing list...
http://bio.net/bionet/mm/biochrom/2002-March/002670.html
*  Genetic Sequencing May Not Be Ready To Become Routine | WUKY
Genetic Sequencing May Not Be Ready To Become Routine. WUKY. Local News Sources. WUKY HD-1. WUKY HD-2. WUKY HD-3. Gallery Hop. KY Theatre Classic Movie Series. WUKY presents... tadoo Lounge. About WUKY. Gallery. Support Us. Support WUKY Through Amazon. Local News Sources. WUKY HD-1. WUKY HD-2. WUKY HD-3. Originally published on March 11, 2014 6:51 pm Transcript ROBERT SIEGEL, HOST: This is ALL THINGS CONSIDERED from NPR News. Getting your entire genetic code deciphered is the cutting edge of medicine. NPR's Rob Stein joins us now to talk about one of the first carefully done studies aimed at answering that question. And, Rob, why don't you back up first and explain just what it means to decipher somebody's entire genetic code. BLOCK: And that was the idea behind this study that I mentioned, trying to figure out the reality here. It's only covered in very specific situations where somebody is using it for a specific medical purpose, like for somebody who has a mysterious condition that nobody can diagnose, or ...
http://wuky.org/post/genetic-sequencing-may-not-be-ready-become-routine
*  ExoSAP-IT reagent: one step to accurate sequencing results | AffymetrixCatalogNav
ExoSAP-IT reagent: one step to accurate sequencing results. Affymetrix. Products. Products. USB Molecular Biology Reagents. Community. Affymetrix Microarray Solutions. About Affymetrix. Contact Us. Products Microarray Solutions Microarray Solutions View all DNA Analysis Solutions Genome-Wide Genotyping for Human Disease Research. RNA Analysis Solutions 3' IVT Expression Analysis. PCR PCR View all End Point PCR DNA Polymerases. PCR Purification. PCR Clean Up View all products >. RT-PCR DNA Polymerases. PCR Purification. Molecular Biology Enzymes Molecular Biology Enzymes View all Binding Proteins. DNA Polymerases. Molecular Biology Kits and Reagents Molecular Biology Kits and Reagents View all Cellular Manipulation View all products >. Home > Promotions > ExoSAP-IT reagent: one step to accurate sequencing results. 100% sample recovery with one-tube, one-step PCR clean-up - no loss of PCR products, regardless of the fragment size. ExoSAP-IT reagent is designed for simple, quick PCR clean-up for downstream a...
http://affymetrix.com/estore/browse/promotionLandingPage.jsp?promotionId=promo610016&navModeValue=35689&isHtmlStatic=true&aIdValue=usbNav&cmpid=USBGENseq128
*  [Bioperl-guts-l] [Bug 2630] Bio::LocatableSeq range validation does not work with translated sequen
Bio::LocatableSeq range validation does not work with translated sequence coordinates. Bio::LocatableSeq range validation does not work with translated sequence coordinates bugzilla-daemon at portal.open-bio.org bugzilla-daemon at portal.open-bio.org. Sun Nov 16 01:06:13 EST 2008. Previous message: seq_inds overhaul Next message: Bio::LocatableSeq range validation does not work with translated sequence coordinates Messages sorted by:. http://bugzilla.open-bio.org/show_bug.cgi?id=2630 ------- Comment #2 from cjfields at bioperl.org 2008-11-16 01:06 EST ------- In reply to comment #1 Initial mapping implementation added to subversion, which takes a two element array or array ref with a parameter and returns a two element array. The two elements are integers not typechecked yet which indicate: 1 # of residues which map to... 2 # of positions based on the LocatableSeq start/end, or the calculated length of the sequence - gaps A translated sequence mapping to nucleotide coordinates would be , while a reverse-trans...
http://bioperl.org/pipermail/bioperl-guts-l/2008-November/027934.html
*  sequencer
... jxli sonytoad at hotmail com sat sep est previous message sequencer next message pooling run folders messages sorted by i think is absolutely better due to abi technology we have a abi and had ever tested a ceq for months we got some good sequence results from ceq as well as our abi and megabace the big problem of ceq is that its laser and fluorescence is very different so you can use reagent only from beckman abi is the update version of abi you know we use many different reagent on abi abi or and these reagent are come from different company such as abi bigdye or amersham et the only excuse to choose ceq is its price if you need more throughput megabace is another choice lijx beijing genome institute www genomics org cn qimin chao qchao at morphotek com wrote in message news n dc fq at mercury hgmp mrc ac uk hi all we are purchasing a new low end capillary sequencing machine and have the choice of abi and beckman ceq anyone has experience with both machines and what are the pros and cons qimin chao ph ...
http://bio.net/bionet/mm/autoseq/2001-September/002301.html
*  Gene density
... in genetics the gene density of an organism s genome is the ratio of the number of genes per number of base pairs usually written in terms of a million base pairs or megabase mb the human genome has a gene density of genes mb while the genome of the c elegans roundworm is estimated to have seemingly simple organisms such as bacteria and amoebas have a much higher gene density than humans bacterial dna has a gene density on the order of genes mb this is due several factors including that the fact that bacterial dna has no introns there are also fewer codon s in bacterial genes see also c value enigma references category genetics category genes...
https://en.wikipedia.org/wiki/Gene_density
*  www.genomebiology.com - Figure 4
www genomebiology com figure resolution standard high figure read coverage depth for models overlapping cdna loci and models not overlapping cdnas the distribution of the average depth log on all exonic nucleotides of the models is plotted for models overlapping cdnas on of their nucleotides green and models not overlapping cdnas black the y axis corresponds to the percentage of models in each bin bin width is denoeud et al genome biology r doi gb r download authors original image...
http://genomebiology.com/2008/9/12/R175/figure/F4
*  DNA SEQUENCERS FOR SALE
http://bio.net/bionet/mm/chlamy/1997-July/000886.html
*  Molecular epidemiology
... In 1993, around the same time as Schulte and Perera published their book, there was also the formation of the International Molecular Epidemiology Task Force IMETAF. 6 Another example, which also reflects how the field of Cancer Research has probably been the most proactive in embracing the term Molecular Epidemiology in a broadest sense was the formation around the same time of Molecular Epidemiology Group of the American Association of Cancer Research MEG/AACR.,. Conceptually, each individual has a unique disease process different from any other individual "the unique disease principle", Ogino S, Lochhead P, Chan AT, Nishihara R, Cho E, Wolpin BM, Meyerhardt AJ, Meissner A, Schernhammer ES, Fuchs CS, Giovannucci E. considering uniqueness of the exposome and its unique influence on molecular pathologic process in each individual. Interdisciplinary education to integrate pathology and epidemiology: Towards molecular and population-level health science. Furthermore, the concept of disease heterogeneity ap...
https://en.wikipedia.org/wiki/Molecular_epidemiology
*  Molecular Epidemiology of NHL and CLL (Supplement 1 to R01 CA92153) - Epidemiology of Lymphoma an
Molecular Epidemiology of NHL and CLL Supplement 1 to R01 CA92153 - Epidemiology of Lymphoma and Leukemia Research Program - Mayo Clinic Research. Patient Care & Health Info. Quality Care Find out why Mayo Clinic is the right place for your health care. Departments & Centers. Medical Departments & Centers. Research Centers & Programs. About Mayo Clinic. Meet the Staff Find a directory of doctors and departments at all Mayo Clinic campuses. Find Clinical Trials. Search Publications. Research and Clinical Trials See how Mayo Clinic research and clinical trials advance the science of medicine and improve patient care. Mayo Medical School. Mayo School of Graduate Medical Education. Professional Services Explore Mayo Clinic’s many resources and see jobs available for medical professionals. Products & Services. Mayo Clinic Health Letter. Medical Products. Continuing Education for Medical Professionals. Giving to Mayo Clinic. Centers and Programs Epidemiology of Lymphoma and Leukemia Research Program. Faculty and St...
http://mayo.edu/research/centers-programs/epidemiology-lymphoma-leukemia-research-program/for-researchers/molecular-epidemiology-nhl-cll-supplement-1-r01-ca92153
*  .. Some Practical ARP Poisoning with Scapy, IPTables, and Burp .. Getting an HTTP proxy in the Mid
http://webstersprodigy.net/2012/07/06/some-practical-arp-poison-attacks-with-scapy-iptables-and-burp/
*  CDC | TB | Genotyping | Chap 2 | Genotyping Used to Improve Contact & Cluster Investigations
Genotyping Used to Improve Contact Cluster Investigations. Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options. Guide to the Application of Genotyping to Tuberculosis Prevention and Control. Tuberculosis Genotyping Case Studies: How TB Programs Have Used Genotyping Genotyping Used to Improve Contact and Cluster Investigations The Massachusetts Department of Public Health recently reported on their universal genotyping program and provided evidence that genotyping strengthened both their contact and cluster investigations Miller 2002. Relationship or location of presumed transmission for 25 patients with suspected epidemiologic links detected during contact investigations that were not confirmed by subsequent genotyping results — Massachusetts, 1996–2000. From 1996 through 2000, Massachusetts used routine contact investigations to identify 92 epidemiologic links between 984 persons with TB. In addition to identifying misle...
http://cdc.gov/tb/programs/genotyping/Chap2/2_TBCase_6Improve_Contact.htm
*  Where global solutions are shaped for you | Disarmament | Convention on Cluster Munitions
UNOG - The United Nations Office at Geneva I am a ... Conference on Disarmament. Convention on Cluster Munitions. Taking Forward Multilateral Nuclear Disarmament Negotiations. Other Issues Related to Disarmament. Conference on Disarmament. Convention on Cluster Munitions. Taking Forward Multilateral Nuclear Disarmament Negotiations. Other Issues Related to Disarmament. Convention on Cluster Munitions. The Convention on Cluster Munitions was concluded by the Dublin Diplomatic Conference on Cluster Munitions at Dublin on 30 May 2008. The Convention is the result of the Oslo-process, a diplomatic process that included States, civil society, the International Committee of the Red Cross as well as the United Nations. Following up on the progress made at the Lima Conference May 2007, the Viena Conference December 2007, and the Wellington Conference February 2008, in May 2008 107 participating states agreed to adopt the text of the new convention at the Dublin Conference on cluster munitions. “Conclude by 2008 a l...
http://unog.ch/80256EE600585943/(httpPages)/F27A2B84309E0C5AC12574F70036F176?OpenDocument
*  [Beowulf] SGI to offer Windows on clusters
sgi to offer windows on clusters sgi to offer windows on clusters jim lux james p lux at jpl nasa gov wed jan pst previous message sgi to offer windows on clusters next message sgi to offer windows on clusters messages sorted by at am joe landman wrote mikhail kuzminsky wrote in message from mikael fredriksson mike at etek chalmers se mon jan this article sgi to offer windows on clusters might be worth reading http www computerworld com action article do command viewarticlebasic articleid source nlt pm nlid any comments in any case it s bad news sgi has solid reputation in hpc and university world and may be somebody will be tempted but it s interesting w which prices sgi will sell their clusters hope that the price will be much more higher than for sgi linux clusters my understanding of pricing for the windows portion is that it adds as an os usd to each node so for a node machine this is an extra k usd tax added on doesn t include the absolutely necessary antivirus anti spyware probably wouldn t be that exp...
http://beowulf.org/pipermail/beowulf/2007-January/017196.html
*  Results for 2012 CRL Under 15 Country Championships - Foster - FOX SPORTS PULSE
2012 CRL Under 13's Country Cup Final 2012 CRL Under 18 Regional Championships 2012 Under 14 Cluster 6, 7, 16 & Illaw 2012 Under 14 Cluster Group 9, 10, 11 & 20 2012 Under 13 Cluster 6, 7, 16 & Illaw 2012 Under 13 Cluster Group 9, 10, 11, & 20 2011 CRL Tier 1 Senior Championships 2011 CRL Under 16 Cup Country Championships 2011 CRL Tier 2 Senior Championships 2011 CRL Under 16's Trophy Country Championships 2011 CRL Under 16's Plate Country Championships 2011 CRL Under 18 Championship 2011 CRL Under 15's Cup Country Championships 2011 CRL Under 15's Trophy Country Championships 2011 CRL Under 15's Plate Country Championships 2011 Under 14's Cluster Groups 1, 2, 3, 18 2011 Under 14's Cluster Groups 4, 14, 19, 21 2011 Under 14 Cluster 6,7,16 Illaw 2011 Under 14's Cluster Groups 9, 10, 11 & 20 2011 Under 14 Cluster Cen Coast, New & Maitland 2011 Under 13's Cluster Groups 1, 2, 3, 18 2011 Under 13's Cluster Groups 4, 14, 19, 21 2011 Under 13 Cluster 6,7,16 Illaw 2011 Under 13's Cluster Groups 9, 10, 11 & 20 2011 ...
http://foxsportspulse.com/comp_info.cgi?a=ROUND&round=-1&client=7-4973-0-218119-0&pool=1
*  [Beowulf] SGI to offer Windows on clusters
SGI to offer Windows on clusters. SGI to offer Windows on clusters Douglas Eadline deadline at eadline.org. Previous message: SGI to offer Windows on clusters Next message: Sun Releases Fortress Implementation Messages sorted by:. My understanding of pricing for the windows portion is that it adds as an OS $500USD to each node. So for a 32 node machine, this is an extra $16k USD tax added on. Doesn't include the absolutely necessary antivirus, anti-spyware, ... Probably wouldn't be that expensive, especially if you boot the same image on all nodes of the cluster. Update one, update all. Is this Windows clustering version, too. I just finished writing an article about the Tyan PSC for Linux magazine. It has 10 quad-core Xeons, GigE, Infiniband, a KVM all in small 21 x 14 x 28 52.7 cm x 35.6 cm x 70 cm package it weighs 150 pounds. They are selling a optional five node version of WCCS for $2,345 $469/node About WCCS. I believe MS is concentrating on bundled applications to sell the WCCS. I think they and others...
http://clustermonkey.net/pipermail/beowulf/2007-January/041555.html
*  2 projects tagged "cluster analysis" – Freecode
... Effective 2014-06-18 Freecode is no longer being updated content may be stale. Learn More. Articles. Browse Projects by Tag. Blog. Sites. Mac OS X. Mobile. Unix. 2 projects tagged cluster analysis. Tags. cluster analysis 2. Data Mining 1. heuristics 1. machine classif.. Diagnostics 1. Clustering 1. SysAdmin 1. hierarchical cl.. 1 Licenses. GPLv2 1. Apache 2.0 1. Programming languages. SQLite 3 1. Bash shell scri.. HTML 1. Java 1. Operating systems. Linux 1. Mac OS X 1. Project Name. Updated 24 Aug 2013. ClodHopper. P op. 32.34. V it. ClodHopper is a Java library for high-performance clustering of numerical data. It contains clustering implementations such as K-Means, K-Means++, X-Means, G-Means, Fuzzy C-Means, Jarvis-Patrick, and various forms of hierarchical clustering. ClodHopper's clustering implementations take advantage of the host system's concurrent processing ability to speed clustering. The data structures are also very lean to conserve memory usage. ClodHopper is very extensible. If you...
http://freecode.com/tags/cluster-analysis?page=1&with=&without=34485
*  2 projects tagged "cluster analysis" – Freecode
... Effective 2014-06-18 Freecode is no longer being updated content may be stale. Learn More. Articles. Browse Projects by Tag. Blog. Sites. Mac OS X. Mobile. Unix. 2 projects tagged cluster analysis. Tags. cluster analysis 2. Data Mining 1. heuristics 1. machine classif.. Diagnostics 1. Clustering 1. SysAdmin 1. hierarchical cl.. 1 Licenses. GPLv2 1. Apache 2.0 1. Programming languages. SQLite 3 1. Bash shell scri.. HTML 1. Java 1. Operating systems. Linux 1. Mac OS X 1. Project Name. Updated 24 Aug 2013. ClodHopper. P op. 32.34. V it. ClodHopper is a Java library for high-performance clustering of numerical data. It contains clustering implementations such as K-Means, K-Means++, X-Means, G-Means, Fuzzy C-Means, Jarvis-Patrick, and various forms of hierarchical clustering. ClodHopper's clustering implementations take advantage of the host system's concurrent processing ability to speed clustering. The data structures are also very lean to conserve memory usage. ClodHopper is very extensible. If you...
http://freecode.com/tags/cluster-analysis?sort=name&with=&without=
*  Single-particle and cluster levels in 107,108,109Sn populated in the decay of 107,108,109Sb
... all Scitation. AIP Conference Proceedings. AIP Publishing AVS: Science Technology of Materials, Interfaces, and Processing Acoustical Society of America American Association of Physicists in Medicine American Association of Physics Teachers American Crystallographic Association, Inc. Chinese Physical Society Chinese Society of Theoretical and Applied Mechanics Laser Institute of America The Society of Rheology. Email: *. Email: *. I'm an author/editor/contributor to this publication. EVENT EVENTLOG PORTALID aip /PORTALID SESSIONID 1xescxedol049.x-aip-live-02 /SESSIONID USERAGENT CCBot/2.0 http://commoncrawl.org/faq/ /USERAGENT IDENTITYID guest /IDENTITYID IDENTITY LIST guest /IDENTITY LIST IPADDRESS 54.83.67.158 /IPADDRESS EVENTTYPE PERSONALISATION /EVENTTYPE CREATEDON 1443957259266 /CREATEDON /EVENTLOG EVENTLOGPROPERTY ITEM ID http://aip.metastore.ingenta.com/content/aip/proceeding/aipcp/10.1063/1.57350 /ITEM ID TYPE recommendtolibrary /TYPE /EVENTLOGPROPERTY /EVENT. Invalid site public key. AIP Confere...
http://scitation.aip.org/content/aip/proceeding/aipcp/10.1063/1.57350
*  Welcome to Focali Focali
Forests, climate and livelihoods are interconnected Forests can play a vital part for both climate- and poverty issues; in reducing the emissions of greenhouse gases and as a source of income for poor people that depend on the forest for their livelihood. Multifunctional landscapes Many Focali researchers focus on synergies and trade-offs between different land-uses in the landscape, such as interactions between forests and agriculture. It is aimed at reducing emissions of carbon dioxide from forests. Welcome to Focali Focali Forest, Climate, and Livelihood research network is a Swedish research network focusing on forest / bio-energy, climate change and poverty issues. Sep 29, 2015 A new publication by Jenny Friman within our thematic collaboration with SIANI. New brief: Addressing food security climate change with agroforestry, what are the policy options. Sep 29, 2015 A new publication by Eskil Mattsson within our thematic collaboration with SIANI. Water, Forests and People Aug 31, 2015 The Swedish Water H...
http://focali.se/en
*  Carolina Digital Repository - GSEA of murine pathway models versus five human subtypes.
carolina digital repository gsea of murine pathway models versus five human subtypes carolina digital repository home browse browse collections browse departments about about the repository frequently asked questions cdr policies and guidelines outreach and partnerships how to contribute material to the cdr technology overview cdr blog contact contact us request access login advanced search back to results previous next doc gsea of murine pathway models versus five human subtypes date deposited download folders collections biomed central identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors parent collection biomed central title gsea of murine pathway models versus five human subtypes identifier uuid ce c eb a aa db c export metadata oai dublin core pre...
https://cdr.lib.unc.edu/record?id=uuid:453162ce-2c79-47eb-a322-7798aa4db4c8
*  Carolina Digital Repository - (a)
carolina digital repository a carolina digital repository home browse browse collections browse departments about about the repository frequently asked questions cdr policies and guidelines outreach and partnerships how to contribute material to the cdr technology overview cdr blog contact contact us request access login advanced search back to results previous next pdf a date deposited download view folders collections biomed central identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors parent collection biomed central title a identifier uuid c cc af b be a ab dcfac f cf export metadata oai dublin core premis events related items neighbors in this collection folder pdf this analysis was used to determine a human pdf this unsupervised cluster analysis is based upon doc gsea of murine pathway models versus clinical er pdf a pdf samples are colored according to mouse model from tiff histological characterization of six different doc gsea of murine p...
https://cdr.lib.unc.edu/record?id=uuid:0c6cc0af-63b4-4be2-a6ab-dcfac52f92cf
*  .. Dallaire calls for transparency as cluster bomb bill moves forward .. Related Stories: .. electi
Dallaire left Wednesday’s Standing Senate Committee on Foreign Affairs and International Trade meeting frustrated due to a misunderstanding during the amendment reading on Bill S-10, an Act to Implement the Convention on Cluster Munitions. But confusion during the reading of Dallaire’s two-part amendment to Section 11 of the bill led to a heated exchange during the meeting. Section 11 would legally exempt Canadian forces from Article 21 of the Convention, which prohibits member states from using cluster munitions in joint military operations with states not party to the Convention. Dallaire’s amendments called for a complete ban of the use of cluster munitions — even in joint operations. Andreychuk and the other Conservative committee members were not available for comment Tuesday. While Dallaire applauds the effort to ratify the Convention on Cluster Munitions, which Canada signed in 2008, he said the exemptions outlined in S-10 defeat the purpose of the convention, which calls for the complete ban of th...
http://ipolitics.ca/2012/11/13/dallaire-calls-for-transparency-as-cluster-bomb-bill-moves-forward/
*  Newest 'cluster' Questions - Unix & Linux Stack Exchange
Newest 'cluster' Questions - Unix & Linux Stack Exchange. Unix Linux. Help Center Detailed answers to any questions you might have. Unix Linux Questions. Tagged Questions info newest frequent votes active unanswered. 0 votes. 1 answer. 14 views. shell-script cluster asked Sep 20 at 16:31. 4 0 votes. 0 answers. 31 views. cluster batch-jobs cluster-ssh asked Sep 18 at 20:26. 2 votes. 1 answer. 23 views. bash ssh cluster asked Sep 18 at 15:39. 1 answer. 35 views. python scheduling cluster external-hdd asked Sep 15 at 16:58. 4 -1 votes. 1 answer. 58 views. ubuntu 64bit cluster 32bit asked Sep 14 at 21:06. 0 answers. 53 views. centos rhel cluster pacemaker asked Sep 1 at 6:41. 4 0 votes. 0 answers. 8 views. 1 answer. 32 views. python performance top cluster load asked Aug 7 at 21:28. 0 votes. 1 answer. 112 views. Cluster is up, every ... linux kernel cluster distributed-filesystem asked May 10 at 2:25. How to benchmark performance and ensure high-availability in a CentOS 7 cluster. centos cluster high-perf...
http://unix.stackexchange.com/questions/tagged/cluster
*  HCV infection cluster help
On the definition of "clusters" and "related sequences" in the HCV database The "exclude related" function selects only the first sequence usually the one with the lowest accession number from each cluster of epidemiologically related sequences from one genomic region, and discards the rest. To get an overview of which clusters have been defined in the database, select "Clusters" in the "Other fields" pulldown menu and type an underscore character in the search field. This will list all defined clusters in the database, and the patients and sequences they are defined on. To select a single cluster for example the cluster associated with patient "Recip 1", a recipient of an infected blood transfusion, type "Recip 1" in the Patient Code field, select "Cluster name" in the Other Fields pulldown and put a star * in the search field; this will list all clusters this patient is a part of. The "Exclude related" function only works when the search has been on a certain genomic region. This is because it makes littl...
http://hcv.lanl.gov/content/sequence/HelpDocs/cluster-help.html
*  www.biomedcentral.com - Figure
www biomedcentral com figure figure the graph shows a subnetwork of proteins linked to stress responses in p falciparum it was detected using the mine plug in for cytoscape which uses an agglomerative approach to search the topology of large networks for significant clusters the visualization is as for figure lilburn et al bmc genomics suppl s doi s s...
http://biomedcentral.com/1471-2164/12/S5/S9/figure/F3
*  Page 2 | Hitting the Cluster Wall - A Study in Cluster Optimization | Projects | Features
Hitting the Cluster Wall - A Study in Cluster Optimization. Site News. Projects Hitting the Cluster Wall - A Study in Cluster Optimization. Article Index Hitting the Cluster Wall - A Study in Cluster Optimization. The GNU/Atlas library and the fact that we are using TCP based MPI libraries. As mentioned, TCP use buffers. Previous tests using Netpipe TCP showed a 29 useconds latency and a 66 MBytes/sec throughput. Before, seeing the effect on HPL, it may be useful to see the difference between GAMMA-MPI, LAM/MPI, and MPICH1. Fortunately, the Netpipe benchmark has an MPI version. Figure One: Throughput vs block size for MPI-GAMMA, LAM/MPI, and MPICH1. Table One: NETPIPE Latency Results MPICH48 MPI Version Latency LAM 41. Figure Two: Latency Signature for MPI-GAMMA, LAM/MPI, and MPICH1. There was a problem with memory space, however. In the case of HPL, the problem size is smaller and thus the less GFLOPS are possible. None the less, it was possible to run a problem size of 11650 successfully. To see the real ef...
http://clustermonkey.net/Projects/hitting-the-cluster-wall-a-study-in-cluster-optimization/Page-2.html
*  Instrument Cluster Store | Instrument Cluster Replacement & Repair
instrument cluster store instrument cluster replacement repair call us at repair cloning service faq customer service need a replacement instrument cluster please call us at we can help you search our inventory the nation s largest to find the correct one for your application repair we provide only the most reliable speedometer repair solutions including speedometer calibration and mileage correction services to ensure that your gauge is working properly and accurately learn more cloning service we provide full cloning services for vehicles that hold the immobilizer inside the cluster cloning involves transferring the immobilizer data from you old cluster to your replacement cluster learn more the ics advantage buy with confidence we offer the following advantages free ground shipping on all orders day warranty on every purchase orders generally ship within hours all clusters are tested and approved before selling top notch customer service to help with your purchase home repair cloning service faq customer s...
http://instrumentclusterstore.com/
*  CRUISE CONTROLE - Jeep - FORUM Marques
... The instrument cluster will turn on the ETC indicator for the following reasons: ² Bulb Test - Each time the ignition switch is turned to the On position the ETC indicator is illuminated for about 15 seconds. ² ETC Lamp-On Message - Each time the cluster receives a lamp-on message from the PCM, the ETC indicator will be illuminated. ² Communication Error - If the cluster receives no lamp-on or lamp-off message from the PCM for 20 consecutive seconds, the Malfunction Indicator Lamp MIL is illuminated by the instrument cluster. The MIL remains controlled and illuminated by the cluster until a valid ETC indicator lamp-on or lamp-off message is received from the PCM. ² Actuator Test - Each time the cluster is put through the actuator test, the ETC indicator will be turned on, then off again during the bulb check portion of the test to confirm the functionality of the LED and the cluster control circuitry. The actuator test illumination of the ETC indicator is a function of the PCM. The PCM then sends the pro...
http://forum-auto.com/marques/jeep/sujet2837.htm
*  IC 2391
ic ic infobox open cluster name ic image epoch j constellation vela class open cluster ra dec ic also known as the omicron velorum cluster or caldwell is an open cluster in the constellation vela the persian astronomer al sufi may have first described it about it was found by abbe lacaille and cataloged as lac ii the cluster is about light year s away from earth and can be seen with the naked eye it contains about stars with a total visual magnitude of spread out along arcminutes the cluster appears to be about the same age as the open cluster ic and has a lithium depletion boundary age of about million years see also omicron velorum references external links entry for ic list of open clusters entry for ic category open clusters category vela constellation b...
https://en.wikipedia.org/wiki/IC_2391

DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Pulsenet: PulseNet is a network run by the Centers for Disease Control and Prevention (CDC) which brings together public health and food regulatory agency laboratories around the United States.http://www.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Genetic variation: right|thumbCampylobacter jejuni: Campylobacter jejuni is a species of bacterium commonly found in animal feces. It is curved, helical-shaped, non-spore forming, Gram-negative, and microaerophilic.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Coles PhillipsCampylobacteriosisCampylobacter coli: Campylobacter coli is a Gram-negative, microaerophilic, non-endospore forming, S-shaped bacterial species within genus Campylobacter.Lansing M.Multiple Loci VNTR Analysis: Multiple Loci VNTR Analysis (MLVA ) is a method employed for the genetic analysis of particular microorganisms, such as pathogenic bacteria, that takes advantage of the polymorphism of tandemly repeated DNA sequences. A "VNTR" is a "variable-number tandem repeat".Quellung reaction: The Quellung reaction is a biochemical reaction in which antibodies bind to the bacterial capsule of Streptococcus pneumoniae, Klebsiella pneumoniae, Neisseria meningitidis, Haemophilus influenzae, Page 340 Escherichia coli, and Salmonella. The antibody reaction allows these species to be visualized under a microscope.Gijs Kuenen: Johannes Gijsbrecht Kuenen (born 9 December 1940, Heemstede) is a Dutch microbiologist who is professor emeritus at the Delft University of Technology and a visiting scientist at the University of Southern California. His research is influenced by, and a contribution to, the scientific tradition of the Delft School of Microbiology.BacitracinInfinite alleles model: The infinite alleles model is a mathematical model for calculating genetic mutations. The Japanese geneticist Motoo Kimura and American geneticist James F.Thermal cyclerSCCmec: SCCmec, or staphylococcal cassette chromosome mec, is a mobile genetic element of Staphylococcus bacterial species. This genetic sequence includes the mecA gene coding for resistance to the antibiotic methicillin and is the only known way for Staphylococcus strains to spread the gene in the wild by horizontal gene transfer.SaPI: SaPIs (Staphylococcus aureus or superantigen pathogenicity islands) are a family of mobile genetic elements resident in the genome of some strains of Staphylococcus aureus. Much like bacteriophages, SaPIs can be transferred to uninfected cells and integrate into the host chromosome.Resistome: The resistome is a proposed expression by Gerard D. Wright for the collection of all the antibiotic resistance genes and their precursors in both pathogenic and non-pathogenic bacteria.Neisseria meningitidis: Neisseria meningitidis, often referred to as meningococcus, is a gram negative bacterium that can cause meningitis and other forms of meningococcal disease such as meningococcemia, a life-threatening sepsis. The bacterium is referred to as a coccus because it is round, and more specifically, diplococcus because of its tendency to form pairs.Cutaneous group B streptococcal infection: Cutaneous group B streptococcal infection may result in orbital cellulitis or facial erysipelas in neonates.St. Columcille's Hospital: St. Columcille's Hospital, Loughlinstown is a publichttp://www.Beta-lactamaseGlobal microbial identifier: The genomic epidemiological database for global identification of microorganisms or global microbial identifier (GMI) is a platform for storing whole genome sequencing (WGS) data of microorganisms, for the identification of relevant genes and for the comparison of genomes to detect and track-and-trace infectious disease outbreaks and emerging pathogens. The database holds two types of information: 1) genomic information of microorganisms, linked to, 2) metadata of those microorganism such as epidemiological details.Recombination (cosmology): In cosmology, recombination refers to the epoch at which charged electrons and protons first became bound to form electrically neutral hydrogen atoms.Note that the term recombination is a misnomer, considering that it represents the first time that electrically neutral hydrogen formed.Staphylococcus condimenti: Staphylococcus condimenti is a Gram positive, coagulase-negative member of the bacterial genus Staphylococcus consisting of single, paired, and clustered cocci. Strains of this species were originally isolated from fermenting soy sauce mash and are positive for catalase, urease, arginine dihydrolase, nitrate reductase, beta-galactosidase, and phosphatase activity.Molecular evolution: Molecular evolution is a change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. The field of molecular evolution uses principles of evolutionary biology and population genetics to explain patterns in these changes.Infection Control and Hospital Epidemiology: Infection Control and Hospital Epidemiology is a peer-reviewed medical journal published by the University of Chicago Press. It publishes research on control and evaluation of the transmission of pathogens in healthcare institutions and on the use of epidemiological principles and methods to evaluate and improve the delivery of care, including infection control practices, surveillance, cost-benefit analyses, resource use, occupational health, and regulatory issues.British Poultry Standard: [Poultry Standard.png|thumb|right|The front cover of the 6th Edition of the British Poultry Standards.National Outbreak Reporting System: ==The National Outbreak Reporting System (NORS)==Beam spoilerGene polymorphismPanton-Valentine leukocidinEnterococcus faecium: Enterococcus faecium is a Gram-positive, alpha-hemolytic or nonhemolytic bacterium in the genus Enterococcus. It can be commensal (innocuous, coexisting organism) in the human intestine, but it may also be pathogenic, causing diseases such as neonatal meningitis or endocarditis.Virulence: Virulence is, by MeSH definition, the degree of pathogenicity within a group or species of parasites as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenicity of an organism - its ability to cause disease - is determined by its virulence factors.Streptococcus agalactiae: Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a gram-positive coccus with a tendency to form chains (streptococcus), beta-hemolytic, catalase-negative, and facultative anaerobe. Streptococcus agalactiae is the species designation for streptococci belonging to the group B of the Rebecca Lancefield classification.Health geography: Health geography is the application of geographical information, perspectives, and methods to the study of health, disease, and health care.Amplified fragment length polymorphismPanmixia: Panmixia (or panmixis) means random mating.King C and Stanfield W.NmVac4-A/C/Y/W-135Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Acinetobacter baumannii: Acinetobacter baumannii is a typically short, almost round, rod-shaped (coccobacillus) Gram-negative bacterium. It can be an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospital-derived (nosocomial) infection.Klebsiella pneumoniaPseudomonas exotoxin: The Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa.Leaf scorch: Leaf scorch (also called leaf burn, leaf wilt, and sun scorch) is defined as a browning of plant tissues, including leaf margins and tips, and yellowing or darkening of veins which may lead to eventual wilting and abscission of the leaf.Enzyme Commission number: The Enzyme Commission number (EC number) is a numerical classification scheme for enzymes, based on the chemical reactions they catalyze.List of lighthouses in Spain: This is a list of lighthouses in Spain.Amplified Ribosomal DNA Restriction Analysis: Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic amplification using primers directed at the conserved regions at the ends of the 16s gene, followed by digestion using tetracutter Restriction enzymes.Ladies Open of Portugal: The Ladies Open of Portugal was a women's professional golf tournament on the Ladies European Tour that took place Portugal.Happy Pet: The Happy Pet is a handheld digital pet released in Japan in 2003 by Bandai.Horizontal gene transfer in evolutionCampylobacter concisus: Campylobacter concisus is a Gram-negative, spiral, and microaerophilic bacteria. Motile, with either unipolar or bipolar flagella, the organisms have a characteristic spiral/corkscrew appearance and are oxidase-positive.Bremer IslandPhylogeography: Phylogeography is the study of the historical processes that may be responsible for the contemporary geographic distributions of individuals. This is accomplished by considering the geographic distribution of individuals in light of the patterns associated with a gene genealogy.Smoke deflectorsAB toxin: The AB toxins are two-component protein complexes secreted by a number of pathogenic bacteria. They can be classified as Type III toxins because they interfere with internal cell function.Carbapenem: Carbapenems are antibiotics used for the treatment of infections known or suspected to be caused by multidrug-resistant (MDR) bacteria. Their use is primarily in people who are hospitalized.Chicken as biological research model: Chickens (Gallus gallus domesticus) and their eggs have been used extensively as research models throughout the history of biology. Today they continue to serve as an important model for normal human biology as well as pathological disease processes.Candida tropicalis: Candida tropicalis is a species of yeast in the genus Candida. It is easily recognized as a common medical yeast pathogen,Bacterial capsule: The cell capsule is a very large structure of some prokaryotic cells, such as bacterial cells. It is a polysaccharide layer that lies outside the cell envelope of bacteria, and is thus deemed part of the outer envelope of a bacterial cell.Phenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.

(1/905) Phylogeny and differentiation of species of the genus Gluconacetobacter and related taxa based on multilocus sequence analyses of housekeeping genes and reclassification of Acetobacter xylinus subsp. sucrofermentans as Gluconacetobacter sucrofermentans (Toyosaki et al. 1996) sp. nov., comb. nov.

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(2/905) Emended description of the genus Pantoea, description of four species from human clinical samples, Pantoea septica sp. nov., Pantoea eucrina sp. nov., Pantoea brenneri sp. nov. and Pantoea conspicua sp. nov., and transfer of Pectobacterium cypripedii (Hori 1911) Brenner et al. 1973 emend. Hauben et al. 1998 to the genus as Pantoea cypripedii comb. nov.

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(3/905) Aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet.

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(4/905) Borrelia carolinensis sp. nov., a novel species of the Borrelia burgdorferi sensu lato complex isolated from rodents and a tick from the south-eastern USA.

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(5/905) Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei.

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(6/905) Multi-locus sequence typing of a geographically and temporally diverse sample of the highly clonal human pathogen Bartonella quintana.

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(7/905) Pantoea allii sp. nov., isolated from onion plants and seed.

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(8/905) Yersinia entomophaga sp. nov., isolated from the New Zealand grass grub Costelytra zealandica.

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