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*  Forensic DNA Fingerprinting Kit | Life Science Education | Bio-Rad
Forensic DNA Fingerprinting Kit. Life Science Education. Bio-Rad ... > Products > DNA Analysis Kits and Agarose Gel ... Kits > Forensic DNA Fingerprinting Kit. Forensic DNA Fingerprinting Kit Print. Email. Overview Purchasing ... Forensic DNA Fingerprinting Kit. Home > Life Science Education > Products > DNA Analysis Kits and Agarose Gel Electrophoresis Kits > Forensic DNA Fingerprinting Kit. 1 DNA samples, lyophilized, 60 g each. 1 Sample loading buffer, 5x, 1 ml. 1 Fast Blast DNA stain, 500x, 100 ml. Forensic DNA Fingerprinting Kit Curriculum Fit. In this lab, students observe the effects of two DNA restriction enzymes on a series of plasmid DNA samples. Download the complete Biotechnology Explorer Refresh Kit Components Purchasing Guide. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized using Bio-Rad's revolutionary Fast Blast DNA stain. Read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the labor...
http://bio-rad.com/en-us/product/forensic-dna-fingerprinting-kit
*  GEN News Highlights Related To | GEN
High-Content Screen Fingerprints. Analysis of the RNA-Seq and SCA ... High-Content Screen Fingerprints. Analysis of the RNA-Seq and SCA ... Highly Conserved DNA Repair Proteins Have Role in Cancer...
http://genengnews.com/more/related/gen-news-highlights/4/30748/?page=5
*  Linknovate | Profile for Crossing tech
Linknovate. DNA fingerprinting, DNA barcoding, and next generation ... in plants. DNA sequencing is increasingly used either ... for traditional DNA fingerprinting techniques. We present...
https://linknovate.com/affiliation/crossing-tech-176528/all/
*  Unitus DSpace: AFLP markers for DNA fingerprinting in cattle
: AFLP markers for DNA fingerprinting in cattle. Search DSpace ... : AFLP markers for DNA fingerprinting in cattle. Authors: ... AFLP TM markers for DNA fingerprinting in cattle. Abstract: his...
http://dspace.unitus.it/handle/2067/124
*  FISA - Kenya Forestry Research Institute
in Africa with DNA fingerprints and stable isotopes Details of ... in Africa with DNA fingerprints and stable isotopes ... fisa kenya forestry research institute home contact legal information data privacy help deutsch text in leichter sprache information system for agriculture and food research information platform of the federal and state governments all projects institutions advanced search home projects research institutions overview federal baden württemberg bavaria berlin brandenburg bremen hamburg hesse lower saxony mecklenburg vorpommern north rhine westphalia rhineland palatinate saarland saxony saxony anhalt schleswig holstein thuringia international funding framework programmes expertatlas teaching and research institutes marginal spalte zum inhalt context sensitive search search all the listed ministerial websites with google in one go context sensitive list external websites kenya forestry research institute kefri kenya forestry research institute kefri carry out research in forestry and allied...
http://fisaonline.de/index.php?lang=en&act=research_inst&i_id=2388
*  Can incest within cooperative breeding groups be detected using DNA fingerprinting?
be detected using DNA fingerprinting. DSpace Repository Can ... be detected using DNA fingerprinting. Login. DSpace Home → ... be detected using DNA fingerprinting. McRae, Susan B. ;...
https://repository.si.edu/handle/10088/1056
*  Mission Statement
http://car.org/meetings/carmeetings/committee-materials-archive/2013spring/springmemo/statement/
*  DNA Fingerprinting - DNA Paternity Testing
DNA Fingerprinting - DNA Paternity Testing. DNA FINGERPRINTING - Paternity Testing. DNA Fingerprinting ... is also known as DNA profiling or Genetic Fingerprinting....
http://usattorneylegalservices.com/dna-fingerprinting.html
*  CDC | E. coli | Timeline for Reporting of E. coli Cases
perform a kind of DNA fingerprinting on E. coli O157 ... whether the DNA fingerprint pattern of E. coli O157 bacteria from ... with the same DNA fingerprint are likely to come from the same source...
http://cdc.gov/ecoli/reportingtimeline.htm
*  DNA Electrophoresis Instructional Lab Set 2 | Life Science Education | Bio-Rad
DNA Electrophoresis Instructional Lab Set 2 ... Lab Packages > DNA Electrophoresis Instructional Lab Set 2 ... DNA Electrophoresis Instructional Lab Set 2...
http://bio-rad.com/en-us/product/dna-electrophoresis-instructional-lab-set-2

No data available that match "DNA Fingerprinting"



(1/3442) Tuberculosis outbreaks in prison housing units for HIV-infected inmates--California, 1995-1996.

During 1995-1996, staff from the California departments of corrections and health services and local health departments investigated two outbreaks of drug-susceptible tuberculosis (TB). The outbreaks occurred in two state correctional institutions with dedicated HIV housing units. In each outbreak, all cases were linked by IS6110-based DNA fingerprinting of Mycobacterium tuberculosis isolates. This report describes the investigations of both outbreaks; the findings indicated that M. tuberculosis can spread rapidly among HIV-infected inmates and be transmitted to their visitors and prison employees, with secondary spread to the community.  (+info)

(2/3442) Influence of sampling on estimates of clustering and recent transmission of Mycobacterium tuberculosis derived from DNA fingerprinting techniques.

The availability of DNA fingerprinting techniques for Mycobacterium tuberculosis has led to attempts to estimate the extent of recent transmission in populations, using the assumption that groups of tuberculosis patients with identical isolates ("clusters") are likely to reflect recently acquired infections. It is never possible to include all cases of tuberculosis in a given population in a study, and the proportion of isolates found to be clustered will depend on the completeness of the sampling. Using stochastic simulation models based on real and hypothetical populations, the authors demonstrate the influence of incomplete sampling on the estimates of clustering obtained. The results show that as the sampling fraction increases, the proportion of isolates identified as clustered also increases and the variance of the estimated proportion clustered decreases. Cluster size is also important: the underestimation of clustering for any given sampling fraction is greater, and the variability in the results obtained is larger, for populations with small clusters than for those with the same number of individuals arranged in large clusters. A considerable amount of caution should be used in interpreting the results of studies on clustering of M. tuberculosis isolates, particularly when sampling fractions are small.  (+info)

(3/3442) Evaluation of pulsed-field gel electrophoresis of genomic restriction fragments in the discrimination of Yersinia enterocolitica O:3.

One hundred and six Yersinia enterocolitica serogroup O:3, biotype 4 isolated from human and porcine samples in 1984 and in the years 1993 5 were examined by pulsed-field gel electrophoresis (PFGE). The genomic profiles produced by the enzymes NotI and XbaI were studied. Sixteen (A-P) and 8 (1-8) different pulsotypes were obtained, respectively. By combining the pulsotypes produced by both NotI and XbaI 24 different types were distinguished. The two major types, designated as A1 and B1, comprised 36% of all strains tested. The proportions of pulsotypes A1 and B1 were, 35.9 and 25.6%, respectively, among strains isolated in 1984. The corresponding figures among the strains isolated in 1993-5 were 35.8 and 41.8%. Nine pulsotypes were found only in 1984 and nine only in 1993-5. The proportions of the major pulsotypes, A1 and B1, in human isolates were 42.9 and 35.7% and in porcine isolates 22.2 and 36.1% respectively. Six types were found among both human and porcine isolates, 8 only among human strains and 10 only among porcine strains.  (+info)

(4/3442) Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16S rRNA gene fingerprints and community-level physiological profiles.

The effect of three phenyl urea herbicides (diuron, linuron, and chlorotoluron) on soil microbial communities was studied by using soil samples with a 10-year history of treatment. Denaturing gradient gel electrophoresis (DGGE) was used for the analysis of 16S rRNA genes (16S rDNA). The degree of similarity between the 16S rDNA profiles of the communities was quantified by numerically analysing the DGGE band patterns. Similarity dendrograms showed that the microbial community structures of the herbicide-treated and nontreated soils were significantly different. Moreover, the bacterial diversity seemed to decrease in soils treated with urea herbicides, and sequence determination of several DGGE fragments showed that the most affected species in the soils treated with diuron and linuron belonged to an uncultivated bacterial group. As well as the 16S rDNA fingerprints, the substrate utilization patterns of the microbial communities were compared. Principal-component analysis performed on BIOLOG data showed that the functional abilities of the soil microbial communities were altered by the application of the herbicides. In addition, enrichment cultures of the different soils in medium with the urea herbicides as the sole carbon and nitrogen source showed that there was no difference between treated and nontreated soil in the rate of transformation of diuron and chlorotoluron but that there was a strong difference in the case of linuron. In the enrichment cultures with linuron-treated soil, linuron disappeared completely after 1 week whereas no significant transformation was observed in cultures inoculated with nontreated soil even after 4 weeks. In conclusion, this study showed that both the structure and metabolic potential of soil microbial communities were clearly affected by a long-term application of urea herbicides.  (+info)

(5/3442) Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp.

A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture.  (+info)

(6/3442) Organization of the gene cluster for biosynthesis of penicillin in Penicillium nalgiovense and antibiotic production in cured dry sausages.

Several fungal isolates obtained from two cured meat products from Spain were identified as Penicillium nalgiovense by their morphological features and by DNA fingerprinting. All P. nalgiovense isolates showed antibiotic activity in agar diffusion assays, and their penicillin production in liquid complex medium ranged from 6 to 38 microgram. ml-1. We constructed a restriction map of the penicillin gene cluster of P. nalgiovense and found that the organization of the penicillin biosynthetic genes (pcbAB, pcbC, and penDE) is the same as in Penicillium chrysogenum and Aspergillus nidulans. The pcbAB gene is located in an orientation opposite that of the pcbC and penDE genes in all three species. Significant amounts of penicillin were found in situ in the casing and the outer layer of salami meat during early stages of the curing process, coinciding with fungal colonization, but no penicillin was detected in the cured salami. The antibiotic produced in situ was sensitive to penicillinase.  (+info)

(7/3442) Identification of Epichloe endophytes in planta by a microsatellite-based PCR fingerprinting assay with automated analysis.

Epichloe endophytes are a group of filamentous fungi that include both sexual (Epichloe) and asexual (Neotyphodium) species. As a group they are genetically diverse and form both antagonistic and mutualistic associations with temperate grasses. We report here on the development of a microsatellite-based PCR system for fingerprinting this group of fungi with template isolated from either culture or infected plant material. M13mp19 partial genomic libraries were constructed for size-fractionated genomic DNA from two endophyte strains. These libraries were screened with a mixture of DIG-labeled dinucleotide and trinucleotide repeat probes. Positive clones were sequenced, and nine unique microsatellite loci were identified. An additional microsatellite was serendipitously identified in the 3' untranscribed region of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase gene from N. lolii Lp19. Primers were designed for each locus and a panel of endophytes, from different taxonomic groupings, was screened to determine the degree of polymorphism. On the basis of these results a multiplex assay was developed for strain identification with fluorescently labeled primers for five of these loci. Using this system the size of the products amplified can be precisely determined by automated analysis, and an allele profile for each strain can be readily generated. The assay was shown to resolve endophyte groupings to the level of known isozyme phenotype groupings. In a blind test the assay was used successfully to identify a set of endophytes in planta. A reference database of allele sizes has been established for the panel of endophytes examined, and this will be expanded as new strains are analyzed.  (+info)

(8/3442) A method for estimating nucleotide diversity from AFLP data.

A method for estimating the nucleotide diversity from AFLP data is developed by using the relationship between the number of nucleotide changes and the proportion of shared bands. The estimation equation is based on the assumption that GC-content is 0.5. Computer simulations, however, show that this method gives a reasonably accurate estimate even when GC-content deviates from 0.5, as long as the number of nucleotide changes per site (nucleotide diversity) is small. As an example, the nucleotide diversity of the wild yam, Dioscorea tokoro, was estimated. The estimated nucleotide diversity is 0.0055, which is larger than estimations from nucleotide sequence data for Adh and Pgi.  (+info)


The police are doing free fingerprinting, should i take my toddler?


At our local mall the police are giving out something with the childs photo, dna & fingerprints. What's the pros and cons of doing this? I feel like there just trying to gather dna & photos for future reference like if the child ever comets a crime.
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No. Bugger that. I'll take my own kids prints and pictures and if anything happens to him I'LL have what's required. I'm not giving them pre empted evidence against my kid if they ever were to commit a crime. Just another way to tab and monitor really.


Is it possible to put DNA from another women into one womens egg to cause a pregnancy?


I know they can use DNA form sperm for In vitro fertilization (IVF) but can they use DNA from the egg of another female?
Jill that is Very helpful, do you have a LINK or anything more about this, because i would appreciate that.
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It's technically possible (scientists fertilized mouse eggs using non-sperm DNA as much as 10 years ago), but there are complications that make it impractical.  The sperm doesn't contain only DNA, but it also contains enzymes and chemicals that cause the process of fertilization to take place (the enzymes tell the egg to "get rid of" one copy of it's DNA, then join the sperm and egg DNA, then kick off the process of cell division).  Without those enzymes and chemicals, you'd have a cell containing 2 different sets of DNA, but nothing would happen next.

That being said, scientists are making great progress in combining the DNA of 2 eggs and THEN fertilizing the resulting egg with a sperm -- this is useful if you have a lesbian couple who wants to have a child that is biologically related to both of them.


is it possible for a DNA test to be wrong and the child look just like the alleged father?


Is it possible to have a swab DNA test done and it come back that the alleged father is not the father. However my child looks just like him, as if he spit out. Can someone please help put a stop to my madness.
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I think that it is highly ulikely, but i did see a tv show of a women who had several kids and her dna did not match any other her kids. She was accused of not being the mother and threatened to have the kids taken away from her until she was pregnant again and they watched her give birth to the child and then tested the newborn baby and the dna did not match the mother but obviously she was the mother.. i'm sure possibly it could happen with a father too, but probably super super rare.


Why will chemo therapy cause a tumor cell to enter apoptosis by damaging DNA, but the original damage did not?


If the job of P53 is to initiate apoptosis in light of DNA damage, hence tumor cell, why does it not initiate until chemotherapy creates the DNA damage?
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Good question... 
A common cause of many cancers is the loss of function of tumor suppressor genes, such as p53. Amongst other jobs, p53 can initiate apoptosis as one way to control cells.

The problem "when the cancer first starts" is not so much DNA damage as it is DNA alteration- alterations (such as deletions, addition, translocations, epigenetic changes, etc) that affect how genes are expressed. The body has well-recognized repair systems for damaged DNA, but they look generally for other changes in DNA. These kinds of "damage" are ones that interfere with DNA replication usually. The afore-mentioned alterations usually don't interfere with replication- they are happily replicated. They then get passed along and are not recognized as "damage". Ultimately, many of these alterations cause loss of control of cell growth and division (at least the ones that lead to malignancy). Many chemotherapies take advantage of this uncontrolled cell growth and induce damage of some sort or another in DNA, which can trigger apoptosis.

So, initially the changes that can "sneak" through and cause cancer are not either 1) of the type which would be recognized by a repair mechanism, or 2) are flat-out missed.

Does that make sense?


How would I go about getting child support and a cheap or free dna test?


Im a teen parent and I was wondering how will I go about getting child support. The dad of course denys the baby, which is a typical thing, but I would like to also go about getting a dna test. Can someone please help because my baby is going on 2 and I would like her to at least know who her father is. Please help.
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You need to go to chil services for the child support...and explain to them that you don't have a huge amout of money for a dna test...you could go on the Maury Povich show??


What happens when all parties are swabbed for a DNA test and the tips are touching? Could he be the dad?


My brother went to Walgreen's and bought a DNA test in a box, swabbed all parties and put all the swabs in the same envelope and they were all touching one another. The results came back and one was in the 92 percentile and the other was in the 96 percentile.  Is there a possibility that the swabs were contaminated with his DNA when they touched in the envelope? Does he need to take this DNA test the right way through a reputable company?
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Unless he stuck them into his mouth/nose/ other orifices or handled the tips with his hands he can't have contaminated them. It's more likely that they contaminated each other. The swabs should have been seperatly preserved, I'm afraid your results are useless


Can a sisters DNA determine the paternity of her brothers child?


Just curious since they are brother and sister, how likely will the DNA match. If the potential father isn't available for the testing, could his sister's DNA be used to determine whether the child is biologically his?
Also where can you find tests that will do this?
Also where can you find tests that will do this?
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If the father isn't available, the next best choice would be to test the father's parents (the baby's grandparents). If they aren't available, then a sister or brother of the father could be tested.

This is called an "avuncular" test. IDENTIGENE does these tests for $495. You can call them at 1-888-404-GENE or find them online at www.identigene.com.


How long do you have to wait to do a DNA test on a newborn?


When there is a possiblity of the father being between 2 best friends, they want to do what is right.  But how do you get a DNA fast enough to fill in the right information on the birth certificate and to also give the baby the proper last name?
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you can do a DNA test immediately.  If your hospital will let you wait to send in the certificate then try to do that.  Once you have the test done it only takes about 10 days to get the results back.  If you have to send it in as a requirement then just put your name on it for now and add dads name when you find out.  It will only cost about $30.00 to have a name added.