No data available that match "DNA, Ribosomal"

*  v.41 (1999) - Malacologia. - Biodiversity Heritage Library
*  Targeting RNA Polymerase I with an Oral Small Molecule CX-5461 Inhibits...
Deregulated ribosomal RNA synthesis is associated with uncontrolled cancer cell ... DNA replication and protein translation. Molecular studies demonstrate that CX-5461 ... Targeting RNA Polymerase I with an Oral Small Molecule CX-5461 Inhibits Ribosomal RNA ... Targeting RNA Polymerase I with an Oral Small Molecule CX-5461 Inhibits Ribosomal RNA ......
*  Plus it
... using a DNA thermal cycler (MJ Research, Watertown, MA) and the following specific primer ... integrity of the purified RNA was confirmed by visualization of the 28S and 18S ribosomal ... The identity of the PCR products was established by DNA sequence analysis, as previously ... The identity of PCR products was confirmed by determination of DNA nucleotide sequences. ......
*  Heterorhabditis baujardi sp. n. (Rhabditida: Heterorhabditidae) from Vietnam...
... and DNA characters. Heterorhabditis baujardi sp. n. is morphometrically similar to H. ... tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences * ... DESS: a versatile solution for preserving morphology and extractable DNA of nematodes * ... and DNA characters. Heterorhabditis baujardi sp. n. is morphometrically similar to H. ......
*  Five new species of Rhigonema Cobb, 1898 (Rhigonematida: Rhigonematidae) from...
... tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences * ... DESS: a versatile solution for preserving morphology and extractable DNA of nematodes * ......
*  Tuning sensitivity to IL-4 and IL-13: differential expression of IL-4Rα, IL...
... and TaqMan Ribosomal RNA Control Reagents for detecting the 18S ribosomal RNA (VIC-MGB ... C) Stat6 DNA binding in WT and γc−/− BMDMs. The cells were stimulated as indicated, and ... As expected, IFN-γ has no effect on Stat6 DNA-binding activity in either WT or Jak3−/− ... 1 E, IL-4-induced Stat6 DNA binding is undetectable in Jak3−/− cells at 10 ng/ml, whereas ......
*  cDNA Synthesis Kits - SMART cDNA Synthesis
... oligo dT primer are not added onto genomic DNA or cDNA transcribed from ribosomal RNA, ... DNA Preparation * Methylation Sensitive Restriction Enzymes * MethylEasy DNA Bisulphite ... pHY300PLK DNA (E. coli-Bacillus shuttle vector) * Thermus thermophilus HB8 Genomic DNA ... DNA Extraction from Paraffin-embedded Tissue * Dr. GenTLE Systems (Gene Trapping by ......
*  Plus it
Prism Dye Terminator cycle sequencing system and an ABI 373A DNA sequencer. The modified ... added upstream of the initiator methionine to enhance mRNA binding to the 40 S ribosomal ......
... the kinases TOR and Sch9 and the ribosomal DNA replication fork barrier protein Fob1 ( ... The resultant oxidative damage to proteins, lipids and DNA leads to manifestations of ... Calorie restriction delays age-related oxidative damage to DNA, proteins and lipids as ... 2004). Sirt1 also inhibits apoptosis by deacetylating the DNA repair protein Ku70, ......
*  AtRH57, a DEAD-box RNA helicase, is involved in feedback inhibition of glucose...
Genomic DNA isolation and genotyping of mutant plants. Arabidopsis DNA was isolated from ... Polysome and ribosomal subunit analyses. The 9-day-old WT Col-0 and rh57-1 seedlings ... Sanan-Mishra, N., Pham, X.H., Sopory, S.K. and Tuteja, N. (2005) Pea DNA helicase 45 over ... Seeds of rh57-1, rh57-2 and rh57-3 are T-DNA insertion lines (SALK_008887, CS917749, and ......

No data available that match "DNA, Ribosomal"

(1/10320) In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.

The Didymium iridis DiSSU1 intron is located in the nuclear SSU rDNA and has an unusual twin-ribozyme organization. One of the ribozymes (DiGIR2) catalyses intron excision and exon ligation. The other ribozyme (DiGIR1), which along with the endonuclease-encoding I-DirI open reading frame (ORF) is inserted in DiGIR2, carries out hydrolysis at internal processing sites (IPS1 and IPS2) located at its 3' end. Examination of the in vivo expression of DiSSU1 shows that after excision, DiSSU1 is matured further into the I-DirI mRNA by internal DiGIR1-catalysed cleavage upstream of the ORF 5' end, as well as truncation and polyadenylation downstream of the ORF 3' end. A spliceosomal intron, the first to be reported within a group I intron and the rDNA, is removed before the I-DirI mRNA associates with the polysomes. Taken together, our results imply that DiSSU1 uses a unique combination of intron-supplied ribozyme activity and adaptation to the general RNA polymerase II pathway of mRNA expression to allow a protein to be produced from the RNA polymerase I-transcribed rDNA.  (+info)

(2/10320) p53 represses ribosomal gene transcription.

Induction of the tumor suppressor protein p53 restricts cellular proliferation. Since actively growing cells require the ongoing synthesis of ribosomal RNA to sustain cellular biosynthesis, we studied the effect of p53 on ribosomal gene transcription by RNA polymerase I (Pol I). We have measured rDNA transcriptional activity in different cell lines which either lack or overexpress p53 and demonstrate that wild-type but not mutant p53 inhibits cellular pre-rRNA synthesis. Conversely, pre-rRNA levels are elevated both in cells which express mutant p53 and in fibroblasts from p53 knock-out mice. Transient transfection assays with a set of rDNA deletion mutants demonstrate that intergenic spacer sequences are dispensable and the minimal rDNA promoter is sufficient for p53-mediated repression of Pol I transcription. However, in a cell-free transcription system, recombinant p53 does not inhibit rDNA transcription, indicating that p53 does not directly interfere with the basal Pol I transcriptional machinery. Thus, repression of Pol I transcription by p53 may be a consequence of p53-induced growth arrest.  (+info)

(3/10320) A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre.

Scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. A 16S rDNA dendrogram (1243 bp) showed that the closest species to the new strain MA112/96T was Mycobacterium abscessus. Phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with M. abscessus, Mycobacterium vaccae, Mycobacterium aurum and Mycobacterium austroafricanum. Ribotyping with Pvull restriction distinguished the 5 isolates from the other 12 most closely related species by the major bands at 6.5-7 kb and 13-15 kb. The cell morphology of the new isolates was typical of mycobacteria, electron microscopy revealed a triple-layered cell wall with an irregular electron-dense outer layer. They grew at 10-37 degrees C, with no growth at 45 degrees C in 5 d. The gene encoding the secreted 32 kDa protein, specific to mycobacteria, was detected by PCR. The main whole-cell fatty acids were characterized by high tuberculostearic acid 10Me-C18:0 (17% at 28 degrees C), which increased with increasing growth temperature (22% at 37 degrees C). The other main fatty acids were C18:1 cis9 and C16:0 (21-20% each), followed by, C17:1 cis9 (14%), C16:1 cis10 (8%) and also a high amount of C20 alcohol (9%). alpha-Mycolic acids, keto-mycolates and wax esters were present (C60-C90), MK-9(H2) (90%) and MK-8(H2) were the main menaquinones. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidyl inositolmannosides and diphosphatidylglycerol. Polyamine content was low. G+C content was 72.9 mol%. The new isolates are proposed as a new species, Mycobacterium murale sp. nov. The type strain is MA112/96T (= DSM 44340T).  (+info)

(4/10320) Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis.

A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 16S rDNA sequence analysis this isolate was classified as a treponeme that differed from all Treponema species described previously. The only isolate, DD5/3T, for which the name Treponema brennaborense is proposed, is designated the type strain of the novel species. The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3T exhibits alpha-glucosidase and N-acetyl-beta-glucosaminidase activity and growth is inhibited by rabbit serum. T. brennaborense was phylogenetically most closely related (89.5% 16S rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.  (+info)

(5/10320) RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: a phylogenetic approach.

It has been established that 16S rRNA gene-based phylogeny gives a low resolution between members of the chemoautotrophic ammonia-oxidizing bacteria (AOB) belonging to the beta-subclass of the Proteobacteria. In this study, 12 isolates of AOB were ribotyped, and the sequences of the 16S-23S rDNA intergenic spacer region (ISR) were determined and used in a phylogenetic study. 16S and 23S rDNA ribotyping revealed that the AOB studied contain only one rrn operon per genome, in contrast to most bacteria, which have 5-10 copies of the rRNA genes per genome. It is likely that the presence of only one set of rRNA genes is related to the slow growth of the AOB. The 16S and 23S rRNA genes of the AOB were shown to be arranged in the classical way: a 16S rRNA gene, an ISR and a 23S rRNA gene. Despite the close phylogenetic relationship among the AOB, the relative location of the rRNA genes in the genome appears to vary considerably. The size of the ISR was approximately 400 bp in the Nitrosomonas isolates and 645-694 bp in the Nitrosospira isolates, suggesting a species-specific size difference in the ISR. The ISR contained two potential tRNA genes in the 5' end in all isolates studied. The similarity values between the ISR sequences of the AOB are low (42.9-96.2%) compared with the 16S rDNA sequence similarity values, and therefore the ISR sequences are valuable as a complementary phylogenetic tool in combination with 16S rRNA gene sequences. The phylogenetic analysis of the AOB based on ISR sequences confirms the 16S rRNA gene-based phylogeny but has the benefit of giving a higher resolution.  (+info)

(6/10320) New genus-specific primers for the PCR identification of members of the genera Pseudonocardia and Saccharopolyspora.

Members of the family Pseudonocardiaceae are difficult to identify on the basis of their micromorphology only. The biochemical characterization of each new isolate is a painstaking and time-consuming task which cannot always be undertaken when handling large numbers of strains as is the case in natural product screening programmes. In this study, two sets of genus-specific oligonucleotides were designed which allow rapid detection of members of the genera Pseudonocardia and Saccharopolyspora by means of PCR-specific amplification. The genus specificity of these primers was validated on a wide range of collection strains and the primers were subsequently used to study a group of 106 wild-type isolates that possessed morphological characteristics of the family. Out of this group, 51 strains could be identified as members of the genus Pseudonocardia and only nine isolates could be assigned to the genus Saccharopolyspora. The diversity indicated by whole-cell fatty acid profiles of both wild-type and reference strains was compared with that identified using the oligonucleotide primers. The partial 16S rDNA sequencing of representative wild-type strains was used to validate their genus assignment by PCR-specific amplification. This study shows the industrial usefulness of the application of these direct identification tools as well as the complementary use of two sources of data, PCR-specific amplification results and fatty acid composition, to assess the diversity of a microbial population.  (+info)

(7/10320) Reclassification of Brevibacterium oxydans (Chatelain and Second 1966) as Microbacterium oxydans comb. nov.

Phylogenetic and chemotaxonomic analyses indicate that Brevibacterium oxydans is closely related to species of the genus Microbacterium, namely Microbacterium liquefaciens, Microbacterium luteolum and Microbacterium saperdae. DNA-DNA reassociation values of less than 60% between Brevibacterium oxydans and these three Microbacterium species support the distinctness of this misclassified Brevibacterium species, which is reclassified as Microbacterium oxydans comb. nov.  (+info)

(8/10320) Reclassification of Brevibacterium incertum (Breed 1953) as Desemzia incerta gen. nov., comb. nov.

Phylogenetic analysis of 16S rDNA indicates that Brevibacterium incertum is not a member of the genus Brevibacterium but related to species of the genus Carnobacterium. Hence, Brevibacterium incertum is not a member of the class Actinobacteria but belongs to the phylogenetically defined broad Bacillus-Lactobacillus cluster. Based upon properties that taxonomically clearly distinguishes Brevibacterium incertum from species of the phylogenetic sister genus Carnobacterium, Brevibacterium incertum is reclassified as Desemzia incerta gen. nov., comb. nov.  (+info)

In bacteria, antibiotic resistance and the ability to conjugate usually involve?

a. chromosomal dna
b. plasmid dna
c.ribosomal rna
d. viral dna
e. nuclear dna

I have looked everywhere in my book and can't seem to find this answer.

d as viral dna codes the bacteria that antibiotics fail to kill or they have penetrates into the cell. look under bacterial coding or why antibiotics don't work on viruses

Is it possible to put DNA from another women into one womens egg to cause a pregnancy?

I know they can use DNA form sperm for In vitro fertilization (IVF) but can they use DNA from the egg of another female?
Jill that is Very helpful, do you have a LINK or anything more about this, because i would appreciate that.

It's technically possible (scientists fertilized mouse eggs using non-sperm DNA as much as 10 years ago), but there are complications that make it impractical.  The sperm doesn't contain only DNA, but it also contains enzymes and chemicals that cause the process of fertilization to take place (the enzymes tell the egg to "get rid of" one copy of it's DNA, then join the sperm and egg DNA, then kick off the process of cell division).  Without those enzymes and chemicals, you'd have a cell containing 2 different sets of DNA, but nothing would happen next.

That being said, scientists are making great progress in combining the DNA of 2 eggs and THEN fertilizing the resulting egg with a sperm -- this is useful if you have a lesbian couple who wants to have a child that is biologically related to both of them.

is it possible for a DNA test to be wrong and the child look just like the alleged father?

Is it possible to have a swab DNA test done and it come back that the alleged father is not the father. However my child looks just like him, as if he spit out. Can someone please help put a stop to my madness.

I think that it is highly ulikely, but i did see a tv show of a women who had several kids and her dna did not match any other her kids. She was accused of not being the mother and threatened to have the kids taken away from her until she was pregnant again and they watched her give birth to the child and then tested the newborn baby and the dna did not match the mother but obviously she was the mother.. i'm sure possibly it could happen with a father too, but probably super super rare.

How to get a DNA test when there is 2 different states ?

My daughters father is asking for a DNA test after 8 years though I do not mind giving him one if it means he will finally be a part of her life I cannot seem to find anywhere that we can walk into an office and have it done I don't want some home DNA kit that can easily be altered by swabbing someone else's mouth can anyone help locate a place In Illinois near St. Louis MO and in Virginia near Richmond ?

Star.. You'll get few answers here as you've asked on "Trying to Conceive". Maybe ask again on pregnancy or parenting.

Very best of luck, hope you get the test result you want.

Why will chemo therapy cause a tumor cell to enter apoptosis by damaging DNA, but the original damage did not?

If the job of P53 is to initiate apoptosis in light of DNA damage, hence tumor cell, why does it not initiate until chemotherapy creates the DNA damage?

Good question... 
A common cause of many cancers is the loss of function of tumor suppressor genes, such as p53. Amongst other jobs, p53 can initiate apoptosis as one way to control cells.

The problem "when the cancer first starts" is not so much DNA damage as it is DNA alteration- alterations (such as deletions, addition, translocations, epigenetic changes, etc) that affect how genes are expressed. The body has well-recognized repair systems for damaged DNA, but they look generally for other changes in DNA. These kinds of "damage" are ones that interfere with DNA replication usually. The afore-mentioned alterations usually don't interfere with replication- they are happily replicated. They then get passed along and are not recognized as "damage". Ultimately, many of these alterations cause loss of control of cell growth and division (at least the ones that lead to malignancy). Many chemotherapies take advantage of this uncontrolled cell growth and induce damage of some sort or another in DNA, which can trigger apoptosis.

So, initially the changes that can "sneak" through and cause cancer are not either 1) of the type which would be recognized by a repair mechanism, or 2) are flat-out missed.

Does that make sense?

How would I go about getting child support and a cheap or free dna test?

Im a teen parent and I was wondering how will I go about getting child support. The dad of course denys the baby, which is a typical thing, but I would like to also go about getting a dna test. Can someone please help because my baby is going on 2 and I would like her to at least know who her father is. Please help.

You need to go to chil services for the child support...and explain to them that you don't have a huge amout of money for a dna could go on the Maury Povich show??

How would a 10 year old girl intrepid that her father is not her real father when a DNA test proves it?

How would a 10 year old girl intrepid that her father is not her real father when a DNA test proves it.  That man taught she was his biological daughter.  He will tread her like his own daughter, and will still live in the same household as her mother.

if I understand your question correctly, you are about to tell this girl that a DNA test proved that the man she thought was her father is actually not a genetic relative.
Kids all over the place have to deal with situations like this more and more these days - usually because of adoption, remarriage, step-family arrangements, etc.
The important thing to remember is that you are a happy family together, and he is not going anywhere. He has always been this same person that she always knew, and isn't changing just because of this DNA test.
Nobody likes to be the bearer of bad news, but it is our responsibility to share painful truths with our children when they are ready to process that information. The news is going to be painful no matter when and how they hear it. But you can present the information in a way that minimizes the pain and can provide the girl with a safe place to fall apart as she processes the news. There is no magic age that's just right for a child to hear things like this. But you can take a cue from her - when she asks about topics like this, she is ready to deal with it, or as ready as anybody is likely to get.
Answer the questions asked without going into more detail than she requests. She may want more information, so she will ask more questions. Answer them honestly. Other times, she really will just want to know her birth father’s name or another bit of information, but absolutely nothing else. By giving her the power to regulate how much she is ready to process when, you enable her to set the pace of how quickly she can handle the information.

What happens when all parties are swabbed for a DNA test and the tips are touching? Could he be the dad?

My brother went to Walgreen's and bought a DNA test in a box, swabbed all parties and put all the swabs in the same envelope and they were all touching one another. The results came back and one was in the 92 percentile and the other was in the 96 percentile.  Is there a possibility that the swabs were contaminated with his DNA when they touched in the envelope? Does he need to take this DNA test the right way through a reputable company?

Unless he stuck them into his mouth/nose/ other orifices or handled the tips with his hands he can't have contaminated them. It's more likely that they contaminated each other. The swabs should have been seperatly preserved, I'm afraid your results are useless