Fungal DNA barcoding

Fungal Diversity Survey

Geotrichum candidum

Callistosporiaceae

Allomyces

Physodermataceae

Reference genome

Point mutation

Blastocladiomycota

Physoderma

Phylloporus

Phylloporus leucomycelinus

Fungal isolate

Skeletocutis

Agaricus

Dimargaritales

Galzinia

Nigrospora sphaerica

Lactarius

Fusarium subglutinans

Savoryellaceae

Fungal contamination of contact lenses

Myriococcum

Copper fist

Doassansiopsis

Dolichousnea

Polyploidy

Phytophthora alni

Rebecca Yahr

Pleurotheciaceae

Fungi21

• Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and sequencing of specific DNA sequences and their comparison with sequences deposited in a DNA barcode database such as the ISHAM reference database, or the Barcode of Life Data System (BOLD). (wikipedia.org)
• In this attempt, DNA barcoding relies on universal genes that are ideally present in all fungi with the same degree of sequence variation. (wikipedia.org)
• Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce the confusing multitude of fungus names. (wikipedia.org)
• The success of identification of fungi by means of DNA barcode sequences stands and falls with the quantitative (completeness) and qualitative (level of identification) aspect of the reference database. (wikipedia.org)
• 2012) proposed the ITS region as primary DNA barcode region for the genetic identification of fungi. (wikipedia.org)
• This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
• On the other hand, the molecular analysis allowed identifying several fungal genera in 33 samples, including the toxigenic fungi Alternaria and Aspergillus, which were positively correlated to the plant genus Hypericum. (nih.gov)
• However, the majority of the fungal diversity comprised taxa of rare and intermediate relative abundance, predominately known mesophilic fungi. (nerc.ac.uk)
• In both sampling areas the DNA of opportunistic, phytopathogenic and symbiotic fungi were detected, which might have been introduced by human activities, transported by birds or wind, and/or represent resident fungi not previously reported from Antarctica. (bas.ac.uk)
• Fungal community structure differed between the two lines at harvest, but not subsequently, suggesting that the presence/absence of the resistance gene influences the microbiome at the base of the stem whilst the plant is alive, but that this does not necessarily lead to differential colonisation of the residues by fungi. (biorxiv.org)
• The sequences corresponded to fungal ITS of eight genera of Ascomycota, five genera of Basidiomycota, Rhizopodium (Chytridiomycota), and Mortierella (Fungi incertae sedis) (Table 2). (dna-alkylating.com)
• Characterizing and understanding possible changes to, and survival of, fungal species in environments like the ILMAH is of high importance since fungi are not only potentially hazardous to the inhabitants but could also deteriorate the habitats themselves. (sciencedaily.com)
• With most fungal species yet to be discovered, the extent of the importance of fungi is most likely underestimated. (kew.org)
• Common DNA regions that are sequenced for identifying fungi to a species level include the external transcribed spacer (ETS), small ribosomal subunit (SSU), internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA sequence (5.8S), internal transcribed spacer 2 (ITS2), large ribosomal subunit (LSU), and intergenic spacer (IGS). (msu.edu)
• In this study, DNA sequence data were used to characterize 290 Fusarium strains isolated during a survey of root-colonizing endophytic fungi of agricultural and nonagricultural plants in northern Kazakhstan. (researchgate.net)
• The Venice Museum hosts the largest and best preserved fungal collection in Italy with more than 25,000 samples, representing approximately 6,000 species of fungi including many rare specimens. (berkeley.edu)
• because mushrooms are only produced seasonally and sometimes very rarely, e.g. only once every several years, the resulting database will facilitate identification of fungi present in plants, in the soil, and in the air at all times, simply by comparing DNA information with the one generated by this and other studies. (berkeley.edu)
• The DNA sequence data generated by this project are now available to the public and research communities and can enhance the ability to identify fungi in the environment. (berkeley.edu)
• DNA-based identification of fungi in the environment can aid in the diagnosis of plant and animal diseases, enhance studies of fungal diversity and evolution, and lead to a better understanding of factors influencing nutrient cycling and productivity in forests and agricultural ecosystems. (berkeley.edu)
• Overview of Fungal Infections Fungi are eukaryotic organisms that exist as yeast, molds, or both forms. (msdmanuals.com)
• The Kew databases contains data and resources concerning plants and fungi such as herbarium and fungarium catalogues, a seed information database, a DNA and tissue bank, accepted plant and fungal names, lists of synonyms and medicinal plant names, and much more. (lu.se)

Species24

• The interspecific variation, i.e., the variation between species, in the chosen DNA barcode gene should exceed the intraspecific (within-species) variation. (wikipedia.org)
• Moreover, fungal species can comprise multiple strains that can vary in their morphology or in traits such as carbon- and nitrogen utilisation, which has often led to their description as different species, eventually producing long lists of synonyms. (wikipedia.org)
• Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
• One group, led by researchers at the Weizmann Institute of Science in Israel and the University of California, San Diego (UCSD), reports detecting fungal DNA or cells-typically at low abundance-in 35 different cancer types, with fungal species composition differing among them. (3quarksdaily.com)
• Fungal species boundaries in the genomics era. (uchicago.edu)
• The DNA ATM Formulation sequences of ITS kinds were determined to recognize fungal species that potentially interacted together with the J2 in soil. (dna-alkylating.com)
• Though several fungal species will not be only capable to synthesize ABA they're capable of sensing the hormone applying specific receptors. (dna-pkinhibitor.com)
• Though a number of fungal species are which acts as transcription factor and is really a crucial compound in the abscisic acid signaling not only capable to synthesize ABA they may be capable of sensing the hormone making use of distinct pathway. (dna-pkinhibitor.com)
• In order to determine which fungal species were present and how the composition of the mycobiome changed during human habitation, samples collected at various time points in a 30-day period were characterized. (sciencedaily.com)
• The researchers gene sequenced the samples to show which species of fungus were present and to determine the total (alive and dead) and viable (alive and able to reproduce) fungal populations. (sciencedaily.com)
• With the advent and advancement of DNA based molecular studies over the last few decades, species, genera, families and orders of Ascomycota have been subjected to rapid taxonomic changes. (researchgate.net)
• These fungal species were collected from more than 300 host species in terrestrial habitats of different provinces in Italy. (researchgate.net)
• Moreover, with major environmental threats such as climate change, we may be losing fungal species to extinction before we even discover them in understudied areas like Boyacá. (kew.org)
• 2017). Just a 50-day fungal inventory revealed up to 308 potentially new species not previously included in the global sequence repositories. (kew.org)
• The overall goal of this project is to answer the question of what fungal species can be found in Boyacá, implementing state-of-the-art approaches to analyse fungal diversity and developing a new protocol that is reproducible and easy to disseminate. (kew.org)
• By performing PCR and sequencing the entire ribosomal operon (~10kb) instead of sequencing each region individually through Sanger Sequencing, scientists can gain high quality data in a high throughput manner, providing clarity and resolution of fungal species relatedness. (msu.edu)
• My work as a master's student is focused on investigating the diversity of species in the fungal family Bankeraceae represented in western North America. (botanicgardens.org)
• Scientists are also starting to better understand how fungal viruses move between species. (theconversation.com)
• Traditional methods of identifying fungal exposures in occupational environments, such as culture and microscopy-based approaches, have several limitations that have resulted in the exclusion of many species. (cdc.gov)
• ITS sequencing results in the detection of many fungal species that are either not detected or difficult to identify to species level using culture or microscopy. (cdc.gov)
• While these methods do not provide quantitative measures of fungal burden, they offer a new approach to hazard identification and can be used to determine overall species richness and diversity within an occupational environment. (cdc.gov)
• DNA probes are available for several species. (medscape.com)
• Mitochondrial quality control prevents accumulation of intramitochondrial-derived reactive oxygen species (mtROS), thereby protecting cells against DNA damage, genome instability, and programmed cell death. (lu.se)
• However, underlying mechanisms are incompletely understood, particularly in fungal species. (lu.se)

Bacterial11

• AllPrep Bacterial/Fungal. (qiagen.com)
• Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. (qiagen.com)
• Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. (qiagen.com)
• We assessed the alterations in gut bacterial and fungal populations associated with a history of appendectomy. (frontiersin.org)
• Compared with those in samples from HwoA, the abundance correlation networks in samples from HwA displayed more complex fungal-fungal and fungal-bacterial community interactions. (frontiersin.org)
• Direct interactions with other members of the community involved many fungal and bacterial ASVs (amplicon sequence variants). (biorxiv.org)
• Nail fungus treatments may be failing because what we have identified as a fungal nail is actually a mixed bacterial and fungal infection (polymicrobial onychopathy). (nova.edu)
• A subsequent in vitro investigation against the bacterial DNA gyrase target enzyme revealed a significant potent inhibitory activity of quinoline derivative 14, which can be observed from its IC50 value (3.39 μM). (olmdiagnostics.com)
• Mycetoma has two distinct etiologies: fungal and bacterial. (who.int)
• The resulting immune system abnormalities make people with AD-HIES highly susceptible to infections, particularly bacterial and fungal infections of the lungs and skin. (medlineplus.gov)
• Uncontrolled and/or active systemic infection (viral, bacterial or fungal) b. (who.int)

Diversity13

• Thus, a DNA metabarcoding approach based on internal transcribed spacer 2 (ITS2) region was used to identify the plant sources of 17 PAs/PANOs detected by a validated method in liquid chromatography coupled to mass spectrometry (LC-MS/MS), as well as floral and fungal diversity in 61 bee pollen samples. (nih.gov)
• Despite limitations in identifying some pollen types, these preliminary results suggest that the DNA metabarcoding could be applied in a multidisciplinary approach to give a picture of floral and fungal diversity, which can be sources of natural contaminants in bee pollen and would help to control its safety. (nih.gov)
• We assessed fungal diversity present in glacial from the Antarctic Peninsula using DNA metabarcoding through high-throughput sequencing (HTS). (nerc.ac.uk)
• We assessed soil fungal diversity at two sites on Deception Island, South Shetland Islands, Antarctica using DNA metabarcoding analysis. (bas.ac.uk)
• DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. (bas.ac.uk)
• We showed that the overall fungal diversity changed when humans were present. (sciencedaily.com)
• They showed that the diversity of the mycobiome and the levels of different fungal populations changed during the duration of the experiment. (sciencedaily.com)
• However, to be able to show that increased fungal diversity is a result of human presence, the mycobiome of the occupants will also need to be studied. (sciencedaily.com)
• The goal of the project described here is to assess the unknown fungal diversity in the department (region) of Boyacá in Colombia, and will run for 10 months until March 2018. (kew.org)
• Fungal research in Colombia could unveil similar diversity patterns as a recent study in southern South America (Truong et al. (kew.org)
• Moreover, at a time when fungal biologists and mycology training are decreasing in most academic institutions, we seek to provide training of local researchers in new techniques as well as in general fungal diversity and taxonomy. (kew.org)
• Overall, straw retention improved α-diversity, network structure and fungal community, while reduced soil pathogenic microbes across the entire soil profile. (biomedcentral.com)
• This protocol details an approach for determining fungal diversity within air samples through genomic DNA extraction, amplification, sequencing, and taxonomic identification of fungal internal transcribed spacer (ITS) regions. (cdc.gov)

Infections14

• Prior to studies of and broad institution of antifungal prophylaxis in high-risk preterm infants, the incidence of fungal infections had been rising in infants born at less than 1000 g, with the ensuing resuscitation and survival of more and more infants. (medscape.com)
• For these reasons, fungal infections are often difficult to eradicate in the preterm infant and, in cases of candidemia, central venous catheter removal is critical for clearance and survival. (medscape.com)
• Although these immunocompromised infants are at increased risk during most of their hospital stay, they are at the highest risk of acquiring invasive fungal infections during the first weeks of life, when the most invasive therapies are performed and remain in place. (medscape.com)
• The pathogenesis of fungal infections in preterm infants involves adherence, colonization, and dissemination (as shown in the image below). (medscape.com)
• Pathogenesis and invasive fungal infections in very low birth weight infants. (medscape.com)
• Furthermore, the importance of the accurate and timely diagnosis of fungal infections in defeating AMR has been starkly absent from policy discussions ( 4 ). (cdc.gov)
• We provide 4 examples of specific clinical situations that require greater application of existing fungal diagnostics and improved overall fungal diagnostic capability and are in line with the 95-95 by 2025 Roadmap from the Global Action Fund for Fungal Infections ( 6 ). (cdc.gov)
• Also, while secondary external fungal infections are relatively common in teleosts and elasmobranchs, primary systemic fungal infections are rarely reported. (vin.com)
• For these reasons, fungal infections are often difficult to eradicate in the preterm infant. (medscape.com)
• This article, focuses on noncandidal oral fungal infections (deep mycoses). (medscape.com)
• Although these noncandidal fungal infections are considerably less common than oral candidiasis, they commonly produce subclinical infection, especially pulmonary infections. (medscape.com)
• The oral lesions associated with these deep fungal infections are chronic and progress to form solitary, chronic deep ulcers with the potential for local destruction and invasion and systemic dissemination. (medscape.com)
• Illness in a person with compelling evidence (e.g., culture, histopathology, seroconversion) of a different fungal infection, such as histoplasmosis or coccidioidomycosis, and meeting only non-confirmatory laboratory criteria for blastomycosis should not be counted as a case of blastomycosis since other fungal infections can cause false positive Blastomyces antigen and antibody test results. (cdc.gov)
• The fungal superbug Candida auris causes serious and often fatal infections. (cdc.gov)

Differences in soil fungal communities2

• Furthermore, no-tillage and traditional agricultural systems have significant differences in soil fungal communities [ 25 ], but little is known about fungal communities' response to soil depth in sugarcane cultivation system. (biomedcentral.com)
• As black spruce stands are also covered by ericaceous shrubs, we investigated if differences in soil fungal communities and ericaceous shrubs abundance could explain the differences observed in balsam fir growth and nutrition. (frontiersin.org)

Barcoding2

• Another crucial prerequisite for DNA barcoding is the ability to unambiguously trace the provenance of DNA barcode data back to the originally sampled specimen, the so-called voucher specimen. (wikipedia.org)
• When the identity of a certain taxon (or a genetic sequence in the case of DNA barcoding) is in doubt, the original specimen can be re-examined to review and ideally solve the issue. (wikipedia.org)

Genes3

• The loss of iron led to a approximately 2.5-fold decrease in DNA-binding affinity, indicating that iron was directly involved in SRE1 regulation of iron-uptake genes. (nih.gov)
• PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes. (uchicago.edu)
• To carry out its roles, the STAT3 protein attaches to DNA and helps control the activity of particular genes. (medlineplus.gov)

Amplicon sequence variants1

• We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Chytridiomycota. (bas.ac.uk)

Microbiome1

• This study, from Illinois, focuses on the alteration of the Massasaugas snake skin microbiome by fungal diseases. (msu.edu)

Genera2

• We detected a total of 353,879 fungal DNA reads, representing 94 genera and 184 taxa, in glacial ice fragments obtained from seven sites in the north-west Antarctic Peninsula and South Shetland Islands. (nerc.ac.uk)
• Bands 9 and 15, of which the DNA was most closely associated to the genera Davidiella and Rhizophydium, respectively, had been connected with J2 from all 3 soils, despite the fact that they wereFIG 1 DGGE profiles of fungal ITS fragments amplified from DNA of M. hapla J2 from 3 arable soils and from total soil DNA. (dna-alkylating.com)

Taxa3

• Only 26 (14%) of the total fungal taxa detected were present at all sampling sites. (nerc.ac.uk)
• In the last eight years, we have introduced many novel fungal taxa with numerous new host and country records of ascomycetous microfungi from Italy. (researchgate.net)
• Besides, both sugarcane straw retention and soil depths (topsoil and subsoil) significantly altered the co-occurrence patterns and fungal keystone taxa closely related to straw decomposition. (biomedcentral.com)

Soil10

• F fungal soil communities had been much extra complicated (Fig. 1). (dna-alkylating.com)
• F fungal soil communities were substantially far more complicated (Fig. 1). (dna-alkylating.com)
• A number of fungal ITS forms had been abundant in all replicate DNA samples from J2 of one particular or extra soils but not in the surrounding soil, suggesting precise attachment to the J2 in soil (Fig. 1, bands two, 3, 4, 6, 9, 11, 13, and 15). (dna-alkylating.com)
• A number of the fungal ITS kinds associated with J2 have been also abundant in soil, but the relative band intensity within the profile was higher for the J2 samples than for soil, which indicated an enrichment on J2 (Fig. 1, bands 1, 5, 7, eight, ten, 12, and 14). (dna-alkylating.com)
• The most reproducible patterns had been detected on J2 from replicates with the most suppressive soil Kw, evidencing by far the most distinct fungal attachment when compared with these in the other two soils. (dna-alkylating.com)
• Collect and document fungal specimens and soil samples in the four types of habitats present in Boyacá. (kew.org)
• The purpose of this study was to provide sufficient information on soil fungal community networks and their functions in response to straw retention. (biomedcentral.com)
• Hence, we used quantitative real-time PCR (qRT-PCR), Illumina MiSeq (ITS rRNA) and FUNGuild to examine ITS rRNA gene populations, soil fungal succession and their functions under control (CK) and sugarcane straw retention (SR) treatments at different soil layers (0-10, 10-20, 20-30, and 30-40 cm) in fallow fields. (biomedcentral.com)
• Thus, retaining straw to improve fungal composition, community stability and their functions, in addition to reducing soil-borne pathogens, can be an essential agronomic practice in developing a sustainable agricultural system. (biomedcentral.com)
• High throughput sequencing (HTS) has been employed to investigate fungal community composition in soil, however, our understanding of the function and network of fungal community in a fallow straw retention system is very limited [ 24 ]. (biomedcentral.com)

Specimens4

• Voucher specimens should be clearly labelled as such, including a permanent voucher identifier that unambiguously connects the specimen with the DNA barcode data derived from it. (wikipedia.org)
• Practicing physicians will obtain nail specimens for standard laboratory and DNA analysis. (nova.edu)
• In this study, a Candida-specific real-time PCR assay was used to detect Candida albicans DNA in inoculated whole blood specimens extracted using seven different extraction protocols. (ukhsa.gov.uk)
• To do this, I am attempting to generate DNA barcode data from all 700 specimens. (botanicgardens.org)

Strains1

• The preliminary antimicrobial activity was assessed against a panel of pathogenic microbes including Gram-positive bacteria (Streptococcus pneumoniae and Bacillus subtilis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), and fungal strains (Aspergillus fumigatus, Syncephalastrum racemosum, Geotrichum candidum, and Candida albicans). (olmdiagnostics.com)

Molecular5

• Molecular (DNA) diagnostics, targeting the genome of microorganisms, have shown that there are often more and different pathogens causing nail disease than what we currently identify via cultivation in lab. (nova.edu)
• Also, a molecular docking study of the most active compounds was carried out to explore the binding affinity of the new ligands toward the active site of DNA gyrase enzyme as a proposed target of their activity. (olmdiagnostics.com)
• dPCR Microbial DNA Detection Assays are intended for molecular biology applications. (qiagen.com)
• Advances in the field over the last two decades have led occupational health researchers to turn to molecular-based approaches for identifying fungal hazards. (cdc.gov)
• CDC sequenced nearly 45,000 DNA samples by using Advanced Molecular Detection (AMD) technologies. (cdc.gov)

Sequence5

• Like the flanking 18S and 28S subunits, the 5.8S subunit contains a highly conserved DNA sequence, as they code for structural parts of the ribosome, which is a key component in intracellular protein synthesis. (wikipedia.org)
• Sre1 had sequence similarity to the fungal negative regulators of siderophore biosynthesis. (nih.gov)
• Sre1p specifically bound DNA containing the 5'-(G/A)ATC(T/A)GATAA-3' sequence, and that binding was both iron- and zinc-dependent. (nih.gov)
• In the past few decades the technology needed to sequence and study fungal viruses has improved in leaps and bounds. (theconversation.com)
• The project started in April 2006, and collection of DNA sequence data was completed in 2011. (berkeley.edu)

Amplification2

• Notably, amplification was inhibited in the presence of a high proportion of fungal DNA compared to human DNA (1000 ng: 1 ng) in DNA mixture experiments. (astm.org)
• However, the detection of low titres of Candida DNA in blood from patients with candidaemia requires the use of extraction methods that efficiently lyse yeast cells and recover small amounts of DNA suitable for amplification. (ukhsa.gov.uk)

Infection5

• All of these factors place them at high risk for fungal infection. (medscape.com)
• Within the context of stewardship programs, inadequate attention has been paid to fungal infection as the cause of antibacterial treatment failure. (cdc.gov)
• Accurate diagnosis or exclusion of fungal infection will have a substantial effect on antimicrobial drug usage and on our ability to limit AMR to bacteria. (cdc.gov)
• Candidiasis (candidosis) is by far the most common fungal infection of the mouth (oral cavity). (medscape.com)
• Cross-reactivity is a known problem with the EIA antigen test, and cases known to be infected with another fungal infection should not be counted as blastomycosis cases. (cdc.gov)

Candida1

• More importantly, the YeaStar method enabled C. albicans DNA to be detected with highest sensitivity over the entire range of copy numbers evaluated, and appears to be an optimal method for extracting Candida DNA from whole blood. (ukhsa.gov.uk)

Pathogens1

• Compounds that revealed the best activity were subjected to further biological studies to determine their minimum inhibitory concentrations (MICs) against the selected pathogens as well as their in vitro activity against the E. coli DNA gyrase, to realize whether their antimicrobial action is mediated via inhibition of this enzyme. (olmdiagnostics.com)

Mycotoxins2

• Fungal DNA, allergens, mycotoxins and associations with asthmatic symptoms among pupils in schools from Johor Bahru, Malaysia. (lu.se)
• Take fungal poisons (mycotoxins), for example. (knowthecause.com)

Ribosomal4

• The region is flanked by the DNA sequences for the ribosomal small subunit (SSU) or 18S subunit at the 5' end, and by the large subunit (LSU) or 28S subunit at the 3' end. (wikipedia.org)
• The ribosomal operon consisting of the ETS, SSU, ITS1, 5.8S, ITS2, LSU, and IGS regions typically sequenced individually as fungal barcodes, and associated primers used to amplify various regions via PCR. (msu.edu)
• These sequences are very close together, and are often found clustered in tandem repeats in fungal genomes known as a ribosomal operon (Fig. 1). (msu.edu)
• A diverse representation of the Venice collection was sampled and sent to U.C Berkeley, where a portion of the genome (the ribosomal DNA internal transcribed spacer, or ITS, region) was sequenced and analyzed. (berkeley.edu)

Microbial4

• The dPCR Microbial DNA Detection Assays and the QIAcuity dPCR System enable accurate digital PCR-based quantification. (qiagen.com)
• Our dPCR Microbial DNA Detection Assays can be combined for multiplex analysis. (qiagen.com)
• A comparison between singleplex and multiplex analysis using the dPCR Microbial DNA Detection Assays shows similar and highly precise quantification for all targets (see figure Microbial detection in multiplex dPCR on QIAcuity ). (qiagen.com)
• The dPCR Microbial DNA Detection Assays are intended for use in nanoplate digital PCR. (qiagen.com)

Composition2

• PA/PANO-producing plants were identified in 54% of the PA/PANO-contaminated samples analysed by DNA metabarcoding, which also allowed identifying the overall floral and fungal composition of 56 samples. (nih.gov)
• Human presence in closed habitats that may one day be used to explore other planets is associated with changes in the composition of the fungal community - the mycobiome - that grows on surfaces inside the habitat, according to a new study. (sciencedaily.com)

Soils1

• Fungal community DNA was sequenced from soils in northern, subarctic Sweden. (data.gov.uk)

Primers1

• The present study focuses on possible cross-reaction of fungal DNA with human STR primers that may affect subsequent forensic DNA analysis of forensic samples. (astm.org)

Communities3

• All communities should have access to nonculture fungal diagnostics, which can substantially benefit clinical outcome, antimicrobial stewardship, and control of antimicrobial resistance. (cdc.gov)
• Knowing how fungal communities change in the presence of humans is thus necessary for the development of appropriate countermeasures to maintain habitats like the ILMAH or the ISS and to protect the health of the people who live there. (sciencedaily.com)
• Our results reveal that fungal communities observed under aspen are drivers of balsam fir early growth and nutrition in boreal forest stands and may facilitate ecotone migration in a context of climate change. (frontiersin.org)

Genomic2

• The yield and quality of total nucleic acids were estimated using UV absorbance, and specific recovery of C. albicans genomic DNA was estimated quantitatively in comparison with a reference (Qiagen kit/lyticase) method currently in use in our laboratory. (ukhsa.gov.uk)
• Of the seven extraction protocols evaluated, only the MasterPure yeast DNA extraction reagent kit gave significantly higher total nucleic acid yields than the reference method, although nucleic acid purity was highest using either the reference or YeaStar genomic DNA kit methods. (ukhsa.gov.uk)

Endophytes2

• Fungal endophytes can improve plant tolerance to abiotic stress conditions. (researchgate.net)
• Fungal root endophytes, including the common form group of dark septate endophytes (DSEs), represent different taxonomic groups with potentially diverse life strategies. (researchgate.net)

Analyse1

• Lazaridis was part of a multidisciplinary study that included archaeologists, anthropologists, and bioinformatics specialists, and is the first to analyse DNA data collected from Neolithic individuals across Mesopotamia (South-eastern Turkey and Northern Iraq), Cyprus, the Zagros mountains and Armenia. (natureasia.com)

Barcodes1

• Morphological and culture characteristics are supported by DNA barcodes. (ingentaconnect.com)

Systematics1

• A fundamental problem in fungal systematics is the existence of teleomorphic and anamorphic stages in their life cycles. (wikipedia.org)

Diseases2

• Lack of routine diagnostic testing for fungal diseases exacerbates the problem of antimicrobial drug empiricism, both antibiotic and antifungal. (cdc.gov)
• Fungal viruses have been important in reducing the impact of fungal diseases on chestnuts in Europe. (theconversation.com)

Genome1

• Genome-wide DNA data collected from people who lived in the Fertile Crescent and surrounding areas during the Neolithic period is enabling scientists to piece together the likely population movements and genetic influences on early farmers in the region. (natureasia.com)

Herbarium1

• Kew databases innehåller data och resurser om växter och svampar såsom herbarium- och fungariumkataloger, en fröinformationsdatabas, en DNA- och vävnadsbank, accepterade växt- och svampnamn, listor på synonymer och medicinala växtnamn etc. (lu.se)

Systemic1

• On 25 January, surgery was performed in an attempt to biopsy the internal organs to check for systemic fungal spread, evaluate organs for predisposing conditions such as neoplasia, and remove the left uterus. (vin.com)

Cysteine1

• this response is mediated by oxidation of the cysteine residue on its DNA binding domain, which enhances DNA binding. (lu.se)

Contaminants1

• The results of the present study underscore the importance of carefully analyzing the presence of non-human biological contaminants that may affect DNA typing of environmentally challenged forensic samples to avoid spurious data interpretation. (astm.org)

Diagnosis1

• There are other useful techniques for the diagnosis of mycetoma such as DNA sequencing. (who.int)

Ecology1

• Fungal Ecology 30: 135-138. (plymouth.ac.uk)

Purification1

• it does not describe the procedure for purification of fungal DNA. (cdc.gov)

Binds1

• DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. (qiagen.com)

Onychomycosis1

• This study will test 25-50 individuals seeking treatment for suspected fungal nail disease (onychomycosis). (nova.edu)

Specificity2

• The specificity of these ten STR markers for human DNA was demonstrated. (astm.org)
• The TaqMan PCR assay used to amplify the DNA extracts achieved high levels of specificity, sensitivity and reproducibility. (ukhsa.gov.uk)

Candidiasis1

• Candidiasis is a sporadic fungal disease in livestock. (springer.com)

Distinct1

• Ancient DNA from Mesopotamia suggests distinct Pre-Pottery and Pottery Neolithic migrations into Anatolia. (natureasia.com)

Extraction1

• Collection and extraction of occupational air samples for analysis of fungal DNA. (cdc.gov)

Epidemiology1

• Oilseed rape residues are a crucial determinant of stem canker epidemiology, as they support the sexual reproduction of the fungal pathogen Leptosphaeria maculans . (biorxiv.org)

Closely1

• Some recently discovered fungal viruses are most closely related to viruses that were thought to infect plants only. (theconversation.com)