Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts. (1/38768)

BACKGROUND: A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. METHODS AND RESUTLS: Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae. Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. CONCLUSIONS: Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation.  (+info)

Acinetobacter bacteremia in Hong Kong: prospective study and review. (2/38768)

The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised.  (+info)

Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications. (3/38768)

The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities.  (+info)

Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov. (4/38768)

A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus.  (+info)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (5/38768)

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. (6/38768)

The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).  (+info)

Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization. (7/38768)

The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.  (+info)

Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. (8/38768)

Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T).  (+info)

... bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA ... Research suggests that bacterial DNA binding protein has an important role during DNA replication; the protein is involved in ... The functions of bacterial DNA-binding proteins are not limited to DNA replication. Researchers have been investigating other ... These proteins participate in all DNA-dependent functions; in these processes, bacterial DNA binding proteins have an ...
... some of the donor's chromosomal DNA may also be transferred with the plasmid DNA. The amount of chromosomal DNA that is ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... Heinemann JA, Sprague GF (July 1989). "Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast". Nature ... Classical E. coli bacterial conjugation is often regarded as the bacterial equivalent of sexual reproduction or mating since it ...
Bacterial DNA is placed into the bacteriophage genome via bacterial transduction. In bacterial conjugation, DNA is transferred ... Bacterial recombination is a type of genetic recombination in bacteria characterized by DNA transfer from one organism called ... Base pairs attached to the DNA strands go through an exchange at a Holliday junction. In the second step of bacterial ... Natural transformation is common among pathogenic bacterial species. In some cases, the DNA repair capability provided by ...
The gene used was the 16S ribosomal DNA. The names have been changed to reflect more current nomenclature used by molecular ... Branching order of bacterial phyla (Rappe and Giovanoni, 2003) Branching order of bacterial phyla after ARB Silva Living Tree ... Branching order of bacterial phyla (Battistuzzi et al.,2004) Branching order of bacterial phyla (Gupta, 2001) Branching order ... There are several models of the Branching order of bacterial phyla, one of these was proposed in 1987 paper by Carl Woese. The ...
... is the process in which a segment of bacterial DNA is copied into a newly synthesized strand of ... RNA polymerase moves down the DNA rapidly at approximately 40 bases per second. Due to the quick nature of this process, DNA is ... RNA polymerase has lower fidelity (accuracy) and speed than DNA polymerase. DNA polymerase has a very different proofreading ... Bacterial RNA polymerase is made up of four subunits and when a fifth subunit attaches, called the σ-factor, the polymerase can ...
Woelfle MA, Xu Y, Qin X, Johnson CH (November 2007). "Circadian rhythms of superhelical status of DNA in cyanobacteria". ... Bacterial circadian rhythms, like other circadian rhythms, are endogenous "biological clocks" that have the following three ... Johnson CH, Zhao C, Xu Y, Mori T (April 2017). "Timing the day: what makes bacterial clocks tick?". Nature Reviews. ... 2009). Bacterial Circadian Programs. Springer. p. 333. Dunlap JC, Loros J, DeCoursey PJ (2004). Chronobiology: Biological ...
Natural transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake ... The process involves creating recombinant DNA molecules through manipulating a DNA sequence. That DNA created is then in ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nature Reviews Microbiology. 2 (3): 241-9. doi:10.1038/ ...
ISBN 978-0-87969-382-4. Lampson BC, Inouye M, Inouye S (2005). "Retrons, msDNA, and the bacterial genome" (PDF). Cytogenetic ... Multicopy single-stranded DNA (msDNA) is a type of extrachromosomal satellite DNA that consists of a single-stranded DNA ... or even between DNA polymerases using DNA as a template, versus DNA polymerases using RNA as a template. The catalytic region ... The priming of msDNA synthesis offers a fascinating challenge to our understanding of DNA synthesis. DNA polymerases (which ...
Watford S, Warrington SJ (2018). "Bacterial DNA Mutations". StatPearls. StatPearls Publishing. PMID 29083710. Retrieved 21 ... Phages destroy bacterial cell walls and membrane through the use of lytic proteins which kill bacteria by making many holes ... For example, a ribosomal mutation may protect a bacterial cell by changing the binding site of an antibiotic but may result in ... Recent advances in high-throughput DNA sequencing as a result of the Human Genome Project have resulted in the ability to ...
Other NAD-dependent enzymes include bacterial DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an ... Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. doi:10.1046/j.1365-2958.2001. ... This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. Li et al. have found that NAD+ ... as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove ...
Bacterial DNA can be located in two places: Bacterial chromosome, located in the irregularly shaped region known as the ... the bacterial DNA is not enclosed inside of a membrane-bound nucleus but instead resides inside the bacterial cytoplasm. This ... Most bacterial chromosomes are circular although some examples of linear DNA exist (e.g. Borrelia burgdorferi). Usually a ... So plasmids can be described as extrachromosomal DNA in a bacterial cell. In most bacteria the most numerous intracellular ...
Chromids, formerly (and less specifically) secondary chromosomes, are a class of bacterial replicons (replicating DNA molecules ... Overall, bacterial genome sequencing indicates that roughly 10% of bacterial species have a chromid. It has also been found ... In the bacterial genus Vibrio, the main chromosome varies between 3.0-3.3 Mb whereas the chromid varies between 0.8-2.4 Mb in ... Bacterial genomes divided between a main chromosome and one or more chromids (and / or megaplasmids) are said to be divided or ...
... but is widespread in bacterial genomes, as part of the restriction modification or DNA repair systems. In Escherichia coli, ... If Dam is targeted to a specific known DNA locus, distal sites brought into proximity due to the 3D configuration of the DNA ... In transient transfection experiments, the DNA of those plasmids is recovered along with the DNA of the transfected cells, ... DamID identifies binding sites by expressing the proposed DNA-binding protein as a fusion protein with DNA methyltransferase. ...
specifically identified bacterial DNA as the underlying component of the lysate that elicited the response. Then, in 1995 Krieg ... Bauer S, Wagner H (2002). "Bacterial CpG-DNA licenses TLR9". Toll-Like Receptor Family Members and Their Ligands. Current ... demonstrated that the CpG motif within bacterial DNA was responsible for the immunostimulatory effects and developed synthetic ... "CpG motifs in bacterial DNA trigger direct B-cell activation". Nature. 374 (6522): 546-9. Bibcode:1995Natur.374..546K. doi: ...
Natural transformation is a common bacterial adaptation for DNA transfer that employs numerous bacterial gene products. For a ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... The DNA-uptake process of naturally competent V. cholerae involves an extended competence-induced pilus and a DNA-binding ... V. cholerae has been used in discoveries of many bacterial small RNAs. Using sRNA-Seq and Northern blot candidate sRNAs were ...
The nonfunctional DNA in bacterial genomes is mostly located in the intergenic fraction of non-coding DNA but in eukaryotic ... Non-coding DNA (ncDNA) sequences are components of an organism's DNA that do not encode protein sequences. Some non-coding DNA ... These are regions of the genome where the DNA replication machinery is assembled and the DNA is unwound to begin DNA synthesis ... This is why these length differences are used extensively in DNA fingerprinting. Junk DNA is DNA that has no biologically ...
DNA alone cannot create a viable cell: proteins and RNAs are needed to read the DNA, and lipid membranes are required to ... First Minimal Synthetic Bacterial Cell. Astrobiology Web. March 24, 2016. Yong, Ed (March 24, 2016). "The Mysterious Thing ... These messages did not use the standard genetic code, in which sequences of 3 DNA bases encode amino acids, but a new code ... The 4 watermarks (shown in Figure S1 in the supplementary material of the paper) are coded messages written into the DNA, of ...
Chen I, Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nature Reviews. Microbiology. 2 (3): 241-9. doi: ... up-taken DNA can either integrate with the genome or exist as extrachromosomal DNA. DNA is generally inserted into animal cells ... "Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda ...
With the help of recombinant DNA techniques, the genes encoded for viral or bacterial antigens could be genetically transcribed ... Chen I, Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nature Reviews. Microbiology. 2 (3): 241-9. doi: ... "Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing ... The discovery of DNA and the improvement of genetic technology in the 20th century played a crucial role in the development of ...
Bacterial genomic DNA is not recognized by these restriction enzymes. The methylation of native DNA acts as a sort of primitive ... Ancient DNA methylation reconstruction, a method to reconstruct high-resolution DNA methylation from ancient DNA samples. The ... relying on DNA methylation MethBase DNA Methylation database hosted on the UCSC Genome Browser MethDB DNA Methylation database ... a large proportion of carcinogenic gene silencing is a result of altered DNA methylation (see DNA methylation in cancer). DNA ...
These DNA fragments may then become integrated into the chromosomes of nearby bacterial cells to undergo mutagenesis. This ... Exogenous DNA is DNA originating outside the organism of concern or study. Exogenous DNA can be found naturally in the form of ... The cell surface and the incoming DNA are both negatively charged, so the DNA is coated with lipids. By shielding the DNA and ... Chen, Inês; Dubnau, David (2004). "DNA uptake during bacterial transformation". Nature Reviews Microbiology. 2 (3): 241-249. ...
Siddiqi, O.; Fox, M.S. (June 1973). "Integration of donor DNA in bacterial conjugation". Journal of Molecular Biology. 77 (1): ... Sarathy, P.Vijay; Siddiqi, O. (August 1973). "DNA synthesis during bacterial conjugation". Journal of Molecular Biology. 78 (3 ... Sarathy, P.Vijay; Siddiqi, O. (August 1973). "DNA synthesis during bacterial conjugation". Journal of Molecular Biology. 78 (3 ... Siddiqi, Obaid H. (May 1963). "Incorporation of parental DNA into genetic recombinants of E. coli". Proceedings of the National ...
Transfer of DNA between bacterial cells can occur in three main ways. First, a bacterium can take up exogenous DNA released ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... Mating in bacteria involves transfer of DNA from one cell to another and incorporation of the transferred DNA into the ... In Bacillus subtilis about 40 genes are required for the development of competence and DNA uptake. The length of DNA ...
TRNA, Cloned Human DNA and E. Coli Sequences, Histone Genes and Restriction Enzyme Recognition Sequences. IRL Press. 1989. TRNA ... ISBN 978-0-471-16067-0. Wiedmann, Martin; Zhang, Wei (4 February 2011). Genomics of Foodborne Bacterial Pathogens. Springer ... ISBN 978-1-881299-24-0. Hather, Gregory James (2008). Statistical Analysis of DNA Sequence Motifs and Microarray Data. ... Caetano-Anollés, Gustavo; Gresshoff, Peter M. (16 September 1997). DNA Markers: Protocols, Applications, and Overviews. Wiley. ...
Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature 277:324-325. West, R.W., Jr., R.L. Neve and R.L. ... A bacterial clone synthesizing proinsulin. Proceedings of the National Academy of Sciences of the United States of America, 75( ... Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature 277:324-325. West, R.W., Jr. and R.L. Rodriguez. ... Recombinant DNA techniques: An introduction. Addison Wesley Publishing Co., Reading Mass. Huang, N., Sutliff, T.D., Litts, J.C ...
Chen I, Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nature Reviews. Microbiology. 2 (3): 241-9. doi: ... "Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". ... In 1972, Paul Berg created the first recombinant DNA molecule when he combined DNA from a monkey virus with that of the lambda ... Bacteria can be induced to take up foreign DNA, usually by exposed heat shock or electroporation. DNA is generally inserted ...
... the process in which bacterial DNA is moved from one bacterium to another by a virus (a bacteriophage, or phage). Bacterial ... Natural transformation is a bacterial adaptation for DNA transfer (HGT) that depends on the expression of numerous bacterial ... Chen I, Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nature Reviews. Microbiology. 2 (3): 241-9. doi: ... The frequency of recombination is increased by DNA damage induced by UV-irradiation and by DNA damaging chemicals. The ups ...
Microarrays use complementary DNA probes to bind to possible sRNA loci in intergenic regions. Northern blotting can reveal ... Bacterial sRNAs affect how genes are expressed within bacterial cells via interaction with mRNA or protein, and thus can affect ... Biofilm is a type of bacterial growth pattern where multiple layers of bacterial cells adhere to a host surface. This mode of ... The first bacterial sRNA was discovered and characterized in 1984. MicF in E. coli was found to regulate the expression of a ...
Agrobacterium tumefaciens, from which it was originally discovered, uses this system to send the T-DNA portion of the Ti ... Bacterial secretion systems are protein complexes present on the cell membranes of bacteria for secretion of substances. ... Type II (T2SS) secretion system depends on the Sec or Tat system for initial secretion inside the bacterial cell. From the ... Type III secretion system (T3SS or TTSS) is structurally similar and related to the basal body of bacterial flagella. Seen in ...
RecA has a central role in the repair of replication forks stalled by DNA damage and in the bacterial sexual process of natural ... UvsX is homologous to bacterial RecA. UvsX, like RecA, can facilitate the assimilation of linear single-stranded DNA into an ... Like Rad51, Dmc1 is homologous to bacterial RecA. Some DNA viruses encode a recombinase that facilitates homologous ... Bernstein C, Bernstein H (2001). DNA repair in bacteriophage. In: Nickoloff JA, Hoekstra MF (Eds.) DNA Damage and Repair, Vol.3 ...
Lanka Erich, Wilkins Brian M (1995). "DNA Processing Reactions in Bacterial Conjugation." Annu. Rev. Biochem. 64: 141-69 Matson ... With human DNA ligase, this forms a crystallized complex. The complex, which has a DNA-adenylate intermediate, allows DNA ... At the end of the segment that DNA polymerase acts on, DNA ligase must repair the final segment of DNA backbone in order to ... The nicks allow the DNA to take on a circular shape. Nicked DNA can be the result of DNA damage or purposeful, regulated ...
DNA Seq. 10 (6): 365-77. doi:10.3109/10425170009015604. PMID 10826693. Stolz JF, Oremland RS (1999). "Bacterial respiration of ...
Dorman, C. J.; Corcoran, C. P. (2008). "Bacterial DNA topology and infectious disease". Nucleic Acids Research. 37 (3): 672-678 ...
Birge, E.A. (2006). "15: Site Specific Recombination". Bacterial and Bacteriophage Genetics (5th ed.). Springer. pp. 463-478. ... DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from ... DNA binding sites were finally confirmed in both systems with the advent of DNA sequencing techniques. From then on, DNA ... DNA binding sites can be thus defined as short DNA sequences (typically 4 to 30 base pairs long, but up to 200 bp for ...
UV light damages bacterial DNA and induces filamentation via the SOS response. Starvation can also cause bacterial ... DNA synthesis-inhibiting and DNA damaging antibiotics (e.g. metronidazole, mitomycin C, the fluoroquinolones, novobiocin) ... The SOS response inhibits septum formation until the DNA can be repaired, this delay stopping the transmission of damaged DNA ... Certain bacterial species, such as Paraburkholderia elongata, will also filament as a result of a tendency to accumulate ...
... bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA ... Research suggests that bacterial DNA binding protein has an important role during DNA replication; the protein is involved in ... The functions of bacterial DNA-binding proteins are not limited to DNA replication. Researchers have been investigating other ... These proteins participate in all DNA-dependent functions; in these processes, bacterial DNA binding proteins have an ...
Evidence that bacterial small molecules also target viruses provides fresh insights into how bacteria thwart viral infection. ... Microbes thwart viral infection using agents that can intercalate into DNA. ... Microbes thwart viral infection using agents that can intercalate into DNA. ... Bacterial defence molecules target viral DNA. Bacteria can use specific protein-based strategies to defend individual cells ...
For extraction of nucleic acids from bacterial and fungal cell cultures ... DNA & RNA Purification. Multianalyte & Virus. AllPrep Bacterial/Fungal.... AllPrep Bacterial/Fungal DNA/RNA/Protein Kits. For ... Comparison of Allprep Bacterial/Fungal DNA/RNA/Protein Kits. Features. Allprep Bacterial DNA/RNA/Protein Kit. Allprep Fungal ... Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) ...
... DNA, Mouse Researchers Find. ... Introducing DNA rather than proteins is thus much easier, research-wise, but it has a downside: non-host (i.e. transgenic) DNA ... Each of Recombinetics calves possessed two antibiotic resistance genes, along with other segments of superfluous bacterial DNA ... Ultimately, all DNA editing is really the cutting of DNA by enzymes, called nucleases, that are supposed to act only at ...
Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This ... The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and ... Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This ... The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and ...
... between mobile genetic elements and the bacterial capsule shape the horizontal flow of DNA in an important bacterial pathogen. ... The horizontal transfer of mobile DNA is one of the signature moves of bacterial evolution, but the specific rules that govern ... Bacterial evolution Is the Subject Area "Bacterial evolution" applicable to this article? Yes. No. ... DNA Is the Subject Area "DNA" applicable to this article? Yes. No. ...
TWiM 230: Ancient bacterial DNA In this episode of This Week in Microbiology, control of Campylobacter in raw chicken by zinc ...
For final DNA samples, perform a dilution series using 6 μL 8X DNA loading dye to 3 μL DNA (or 3 μL of the previous dilution) ... For test aliquots, add 2 μL 8X DNA loading dye to 2 μL DNA and load 4 μL ... Adapted from the QIAGEN Genomic DNA handbook and various CTAB procedures: DOE JGI. Current protocols in Molecular Biology (.pdf ... NOTE: Do NOT use pipetting for resuspension-it can cause shearing of the genomic DNA. ...
Column-Pure Bacterial Genomic DNA Isolation Kit from Applied Biological Materials (ABM). Cat Number: D512. UK & Europe ... Column-Pure Bacterial Genomic DNA Isolation Kit , D511 abm dna purification Column-Pure Bacterial Genomic DNA Isolation Kit , ... abm dna purification. Column-Pure Bacterial Genomic DNA Isolation Kit , D511. Rating Required Select Rating. 1 star (worst). 2 ... Column-Pure Blood Genomic DNA Kit , D203-Mini-1 abm dna purification ...
Salmonella enterica Quantified Bacterial DNA Standard. Cat. 28300. To be used as a positive control or PCR quantification ... Salmonella enterica Quantified Bacterial DNA Standard Salmonella enterica has emerged as a significant foodborne pathogen that ... Upon receipt, store Norgens Salmonella enterica Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze- ... Upon receipt, store Norgens Salmonella enterica Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze- ...
... single-molecule and in vivo methods are used to show how ParB locally condenses the bacterial chromosome near the origin and ... DNA binding by the CTD is required for efficient DNA condensation in vitro.. (A) Mean force-extension curves of DNA molecules ... The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for ... The CTD binds DNA via a lysine-rich surface.. (A-C) TBE-EMSAs for the titration of full length ParB and CTD against 147 bp DNA ...
DNA methods are still needed. To date, there have not been any studies which identify the bacterial presence within healthy, ... Interpretive Summary: Characterization of bacterial DNA identified in abscessed and non-abscessed bovine hepatic tissue at the ... Additionally, results indicate that the bacterial community may not necessarily cluster together, according to the liver score ...
AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria ... AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria ... AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria ... AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains ...
Puc 19 Puc Vector Rfp Plasmid Sequence Analysis Software socs1 antibody Strepii Tag Sequence Sumo Sequence Ta Clone Taq Dna ... Puc 19 Puc Vector Rfp Plasmid Sequence Analysis Software socs1 antibody Strepii Tag Sequence Sumo Sequence Ta Clone Taq Dna ...
DNA microarray. Gene expression during growth in rabbit ileal loops. 16. DNA microarray. Gene expression in rice water stools. ... DNA microarray. Growth in dialysis membranes implanted in rats. 18. DNA microarray. Adaptation to growth in dialysis membranes ... Genomic-scale Analysis of Bacterial Gene and Protein Expression in the Host John D. Boyce*. , Paul A. Cullen*, and Ben Adler* ... DNA microarray. Alteration of lipoprotein expression during host adaptation in mice. 20. ...
Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates ... Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates ... title = "Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm ... Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates ...
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Researchers have engineered a protein from bacteria that kills other microbes to change DNA in a previously inaccessible part ... A bacterial toxin enables the first mitochondrial gene editor. Researchers can now change DNA in a previously inaccessible ... Bacterial weaponry has an unexpected use in human cells.. A protein secreted by bacteria to kill other microbes has been re- ... Mutations in mitochondrial DNA cause over 150 distinct syndromes and affect 1,000 to 4,000 children born in the United States ...
Bacterial Agents. Anthrax. Bacillus anthracis is a large, aerobic, gram-positive, spore-forming, nonmotile bacillus. The ... These include genome sequencing that enabled publication of full genomes, recombinant DNA technology, reverse genetic ... The anthrax toxins, like many bacterial and plant toxins, possess the following two components: a cell-binding B-domain and an ... It disrupts cell defense mechanisms that lead to organ injury, uncontrolled bacterial growth, and death. [24] ...
Dive into the research topics of Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis. ... Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis. ...
We assessed the alterations in gut bacterial and fungal populations associated with a history of appendectomy. In this cross- ... The HwA subgroup analysis indicated a trend toward restoration of the HwoA bacterial microbiome over time after appendectomy. ... The HwA subgroup analysis indicated a trend toward restoration of the HwoA bacterial microbiome over time after appendectomy. ... Analysis showed that the gut bacterial composition of samples from HwA was less diverse than that of samples from HwoA and had ...
In this paper, we described a new process that is based on the complexation of DNA with linear polylysine, followed by ... The originality of the process consisted of using mechanic force to elute DNA from the complex. The extraction method showed ... Furthermore, it was proven to be a good solution for removing PCR inhibitors and assuring good DNA recovery yield. ... Although the use of polycation to complex and purify DNA has been described in the literature, elution often requires a high ...
Replication - the action in DNA. Today we will try to introduce you to one of them - replication. When thinking about genetics ... However, although genetics is actually based on genetic material in the form of DNA, many different processes are involved. ... most of us think of the DNA helix. Two lines spinning around each other creating a screw-like shape. ... Milk Bacterial DNA Extraction Kit (50prep) - Favorgen ... Milk Bacterial DNA Extraction Kit (50prep) Catalog number: / ...
Riboflavin binds to DNA and RNA to create cross-links when photoactivated. It appears to be effective in inactivating HIV and ... How prevalent are transfusion-transmitted bacterial infections (TTBIs)?. How are transfusion-transmitted bacterial infection ( ... Bacterial contamination of blood components. Clin Microbiol Rev. 2005 Jan. 18(1):195-204. [QxMD MEDLINE Link]. [Full Text]. ... Insufficient bacterial inocula that may result in a positive culture from the donor product or the recipient but that is not ...
Interventions such as improving oral hygiene may lead to decreased S. aureus carriage by reducing other bacterial species such ... DNA; Deoxyribonucleic acids; Risk analysis; Risk factors; Livestock; Animals; Animal husbandry workers; Animal handlers; Humans ... Staphylococcus aureus; Staphylococcus aureus infection; Infectious agents; Bacteria; Bacterial infections; Nasal cavity; ...
The targets of quinolone activity are the bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and ... Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e., DNA gyrase and topoisomerase IV. Resistance ... Resistance to quinolones has been reported in a variety of important bacterial pathogens, including Escherichia coli, ...
Inicio Todos los cursos CienciaBiologíaFutureLearn Bacterial Genomes: From DNA to Protein Function Using Bioinformatics ... Bacterial Genomes: From DNA to Protein Function Using Bioinformatics. Por: FutureLearn . en: Ciencia, Biología, FutureLearn ... healthcare professionals and all those who are interested in learning about the underlying mechanisms of bacterial disease, DNA ... The opportunity to use online computational tools in the context of bacterial genomes will also be of interest to teachers and ...
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Preserve and Store Bacterial DNA. CloneStable® enables the long-term storage of unpurified bacterial plasmid and genomic DNA. ... DNA & RNA Purification. Biomatrica® offers an innovative alternative to reliance on cold chain logistics ... DNAgard® offers immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping ... Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes ...
  • Upon further investigation, it was discovered that the amino acid composition of HU resembles that of eukaryotic histones, thus prompting further research into the exact function of bacterial DNA binding proteins and discoveries of other related proteins in bacteria. (wikipedia.org)
  • Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. (wikipedia.org)
  • H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)
  • Evidence that bacterial small molecules also target viruses provides fresh insights into how bacteria thwart viral infection. (nature.com)
  • This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. (zymoresearch.com)
  • Norgen's Salmonella enterica Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen's sample preparation technology. (norgenbiotek.com)
  • A protein secreted by bacteria to kill other microbes has been re-engineered to tweak DNA inaccessible to other gene editors, scientists report online July 8 in Nature . (sciencenews.org)
  • A new study has found that areas surrounding active salmon farms in the Broughton Archipelago and the Discovery Islands were 2.72 times more likely to contain DNA from disease-causing viruses and bacteria. (thefishsite.com)
  • As I understand it, naturally competent bacteria carry genes that encode the machinery necessary to facilitate the transport of this DNA across the membrane and into the cell. (stackexchange.com)
  • Can bacteria release free DNA into their environment? (stackexchange.com)
  • Others result from mobile genetic elements snippets of DNA that are able to move between bacteria. (cdc.gov)
  • This study determined the prevalence of bacterial contamination of mobile phones of health workers and the predisposing factors, in order to ascertain the risk of transmission of pathogenic bacteria through mobile phones. (bvsalud.org)
  • Bacterial DNA gyrase, a type II DNA topoisomerase found in all bacteria is a proven target for antibacterial chemotherapy. (bvsalud.org)
  • In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. (wikipedia.org)
  • HU is a small (10 kDa) bacterial DNA-binding protein, which structurally differs from a eukaryotic histone but functionally acts similarly to a histone by inducing negative supercoiling into circular DNA with the assistance of topoisomerase. (wikipedia.org)
  • ParB is an unusual DNA binding protein. (elifesciences.org)
  • I remember reading something about a transformation-specific DNA binding protein that coats the entire length of the DNA strand and prevents degradation. (stackexchange.com)
  • AllPrep Bacterial/Fungal. (qiagen.com)
  • Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. (qiagen.com)
  • Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. (qiagen.com)
  • Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. (zymoresearch.com)
  • We assessed the alterations in gut bacterial and fungal populations associated with a history of appendectomy. (frontiersin.org)
  • Compared with those in samples from HwoA, the abundance correlation networks in samples from HwA displayed more complex fungal-fungal and fungal-bacterial community interactions. (frontiersin.org)
  • Nail fungus treatments may be failing because what we have identified as a fungal nail is actually a mixed bacterial and fungal infection (polymicrobial onychopathy). (nova.edu)
  • It can be caused by a large variety of microorganisms, but has essentially two distinct etiologies: bacterial and fungal. (who.int)
  • In molecular biology, bacterial DNA binding proteins are a family of small, usually basic proteins of about 90 residues that bind DNA and are known as histone-like proteins. (wikipedia.org)
  • Since bacterial binding proteins have a diversity of functions, it has been difficult to develop a common function for all of them. (wikipedia.org)
  • Eukaryotic histones package DNA to help it to fit in the nucleus, and they are known to be the most conserved proteins in nature. (wikipedia.org)
  • These dinoflagellate histone-like proteins replace histone in some dinoflagellates and package DNA into a liquid-crystalline state. (wikipedia.org)
  • in these processes, bacterial DNA binding proteins have an architectural role, maintaining structural integrity as transcription, recombination, replication, or any other DNA-dependent process proceeds. (wikipedia.org)
  • Initially, bacterial DNA binding proteins were thought to help stabilize bacterial DNA. (wikipedia.org)
  • Therefore we can speculate that DNA repair proteins will be prevalent and DNAses will be low in the cell, under these conditions. (stackexchange.com)
  • Here we report two groups of TnpB-like proteins (Fanzor1 and Fanzor2) that are widespread in diverse eukaryotic transposable elements (TEs), and in large double-stranded DNA (dsDNA) viruses infecting eukaryotes. (biomedcentral.com)
  • Fanzor1 proteins are frequently captured by DNA transposons from different superfamilies including Helitron , Mariner , IS4 -like, Sola and MuDr . (biomedcentral.com)
  • Proteins are major chromophores and targets for damage due to their abundance, but the role of proteins in inducing damage to bound DNA, and the effects on DNA-protein interactions is less well characterized. (ku.dk)
  • The study identified the ways in which proteins cross a cell membrane, a finding that could create a scientific foundation for better ways of delivering drugs into cells in the future, or for treating illnesses caused by bacterial toxins. (scitechdaily.com)
  • The full article, published under the title Environmental DNA from multiple pathogens is elevated near active Atlantic salmon farms , is now available via Proceedings of the Royal Society B: Biological Sciences . (thefishsite.com)
  • I am a researcher in bacterial genetics and my interest is in understanding the development of resistance in bacterial pathogens using next generation sequencing techniques. (wellcomeconnectingscience.org)
  • I am a graduate student at the Wellcome Sanger Institute, where I research the virulence and the molecular genetics of bacterial pathogens as part of the Infection Genomics programme. (wellcomeconnectingscience.org)
  • The labs develop and compare DNA patterns from bacterial pathogens submitted by state, Food and Drug Administration, and the U.S. Department of Agriculture laboratories from across the nation. (cdc.gov)
  • Molecular (DNA) diagnostics, targeting the genome of microorganisms, have shown that there are often more and different pathogens causing nail disease than what we currently identify via cultivation in lab. (nova.edu)
  • But FDA's findings are potentially trivial besides another recent discovery about gene-editing: that foreign DNA from surprising sources can routinely find its way into the genome of edited animals. (greenmedinfo.com)
  • Ultimately, all DNA 'editing' is really the cutting of DNA by enzymes, called nucleases, that are supposed to act only at chosen sites in the genome of a living cell. (greenmedinfo.com)
  • Genome copy # = DNA (g) / (g_to_bp const. (zymoresearch.com)
  • This process of inducing virulence in an otherwise harmless bacterium through prophage infection and integration of the prophage genome into the bacterial host genome is called lysogenic conversion 8 . (nature.com)
  • For most commercial applications gene-editing's appeal is simplicity and precision: it alters genomes at precise sites and without inserting foreign DNA. (greenmedinfo.com)
  • Use bioinformatics to probe genomes, to explore and represent DNA and protein sequences. (mooc.es)
  • The course will be of interest to undergraduates, post-graduates, researchers, bioinformaticians, biomedical researchers, microbiologists, healthcare professionals and all those who are interested in learning about the underlying mechanisms of bacterial disease, DNA sequences and protein data, or how to use online analytical tools to probe genomes. (mooc.es)
  • The opportunity to use online computational tools in the context of bacterial genomes will also be of interest to teachers and their 16-18-year-old science and computing students. (mooc.es)
  • Thus, apparently unbeknownst to Recombinetics, adjacent to its edited site were 4,000 base pairs of DNA that originated from the plasmid vector used to introduce the DNA required for the hornless trait. (greenmedinfo.com)
  • The origin of this foreign DNA, as with Recombinetics' calves, was the plasmid vector used in the editing process. (greenmedinfo.com)
  • CloneStable ® enables the long-term storage of unpurified bacterial plasmid and genomic DNA. (sigmaaldrich.com)
  • Solu provides a platform that includes: a "genomics bench in the cloud" for analysing bacterial DNA, a global pathogen database that can match sample DNA to thousands of known strains, and tools for detecting and managing outbreaks. (springwise.com)
  • The aim of this study was to evaluate the performance of a novel multiplex PCR assay for diagnosis of late-onset sepsis and to investigate the value of bacterial DNA load (BDL) determination as a measure of infection severity. (maastrichtuniversity.nl)
  • a 2-year-old boy died.¹ E. coli bacterial infection is a growing concern for public health officials in the North Carolina Department of Health and Human Services and other local health departments. (bartleby.com)
  • In Vibrio cholerae , infection by a bacterial virus (bacteriophage) results in the conversion of non-pathogenic strains to pathogenic strains and this can lead to cholera pandemics. (nature.com)
  • One such example is diphtheria toxin, which is produced by Corynebacterium diphtheriae and causes diphtheria, a serious and potentially fatal bacterial infection of the nose and throat. (scitechdaily.com)
  • An important concept in the evaluation of data regarding transfusion-transmitted bacterial infections (TTBIs) is the definition of a case. (medscape.com)
  • ceptible to urinary tract infections with The second phase of our study included multiresistant bacterial strains [ 7-9 ]. (who.int)
  • The gene-editing of DNA inside living cells is considered by many to be the preeminent technological breakthrough of the new millennium. (greenmedinfo.com)
  • They imply, at the very least, the need for strong measures to prevent contamination by stray DNA, along with thorough scrutiny of gene-edited cells and gene-edited organisms. (greenmedinfo.com)
  • One can back calculate from the amount of genomic DNA to determine genomic copies, and then multiply by a factor of 7 to determine total copies of the 16S rRNA gene. (zymoresearch.com)
  • Scientists report a new gene-editing tool that can target DNA in mitochondria (red). (sciencenews.org)
  • We have investigated the application of bacterial magnetite particles as DNA carriers for microprojectile based gene transfer. (elsevierpure.com)
  • A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of horizontal gene transfer in their natural environments. (stackexchange.com)
  • A blood sample was collected from a peripheral vein and from the central venous catheter (CVC) or the arteriovenous fistula (AVF) and examined for presence of BDNAs by 16S rRNA gene PCR amplification, bacterial growth, and measurement of C-reactive protein and IL-6. (unicatt.it)
  • The DNA quantity and purity were determined using real-time PCR targeting the bacterial 16S rDNA gene. (knaw.nl)
  • The emergence of certain bacterial strains resistant to antibiotics has become a major public health problem, hence the need to develop new antibiotic molecules. (bvsalud.org)
  • 1 report their analysis of an antiviral defence system that can protect more than one bacterial species. (nature.com)
  • Analysis showed that the gut bacterial composition of samples from HwA was less diverse than that of samples from HwoA and had a lower abundance of Roseburia , Barnesiella , Butyricicoccus , Odoribacter , and Butyricimonas species, most of which were short-chain fatty acids-producing microbes. (frontiersin.org)
  • Interventions such as improving oral hygiene may lead to decreased S. aureus carriage by reducing other bacterial species such as Porphyomonas. (cdc.gov)
  • As a result, reactive oxygen species easily damage mitochondrial DNA, causing cells to malfunction and ultimately to die. (medlineplus.gov)
  • Patients on antibiotics had higher relative abundance of phylum Firmicutes with bacterial species, Streptococcus intermedius, Lactobacillus gasseri, Mogibacterium timidum, and Solobacterium moorei, whereas patients without antibiotics had greater amounts of Parvimonas micra and Streptococcus anginosus. (nih.gov)
  • They used FISH probes informed by publicly available bacterial 16S ribosomal RNA databases to search for Fusobacterium species found in some vaginal samples in past studies. (genomeweb.com)
  • The modifications at the CRP dimer interface, and the site-specific DNA strand cleavage are proposed to occur via oxidation of two species Met residues (Met114 and Met189, respectively) to reactive persulfoxides that damage neighbouring amino acids and DNA bases. (ku.dk)
  • The laboratory (18.8%, 21/112) and medical wards (16.1%, 18/112) had the highest bacterial contamination of mobile phones (p=0.041), and more bacterial species were isolated from smartphones (68.8%, n=77/112) than keypad phones (31.2%, n=35/112) (p=0.032). (bvsalud.org)
  • When thinking about genetics, most of us think of the DNA helix. (gentaur.co.uk)
  • However, although genetics is actually based on genetic material in the form of DNA, many different processes are involved. (gentaur.co.uk)
  • Gould, 2011) E. coli 0157 is a member of a large group of bacterial germs that inhibit the intestinal tract of humans and other warm - blooded animals. (bartleby.com)
  • The effectiveness of three commercially available direct DNA isolation kits (Mobio, Fast, Qiagen) and one published direct DNA extraction protocol (Bead) for extracting bacterial DNA from different types of activated sludge was investigated and mutually compared. (knaw.nl)
  • Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. (medlineplus.gov)
  • The remaining genes provide instructions for making molecules called transfer RNA (tRNA) and ribosomal RNA (rRNA), which are chemical cousins of DNA. (medlineplus.gov)
  • Each of Recombinetics' calves possessed two antibiotic resistance genes, along with other segments of superfluous bacterial DNA. (greenmedinfo.com)
  • Mitochondrial DNA contains 37 genes, all of which are essential for normal mitochondrial function. (medlineplus.gov)
  • Standards are QC tested serial dilutions of genomic DNA. (zymoresearch.com)
  • The standards included in the Femto Bacterial DNA Quantification kit are serial dilutions of E. coli genomic DNA. (zymoresearch.com)
  • Two micro-liters of Norgens Salmonella enterica Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Salmonella enterica -specific PCR reaction using Norgens 2X PCR Master Mix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad CFXReal-Time PCR System. (norgenbiotek.com)
  • Norgens Salmonella enterica Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to fewer than 40 copies per reaction. (norgenbiotek.com)
  • This study was aimed to investigate the effect of antibiotics on bacterial diversity in BRONJ tissues. (nih.gov)
  • Two bacterial virulence factors are required for effective induction of apoptosis by extracellular GAS: (i) hyaluronic acid capsule that inhibits bacterial internalization and (ii) secreted cytolysin, streptolysin O (SLO), that forms. (lu.se)
  • Two bacterial virulence factors are required for effective induction of apoptosis by extracellular GAS: (i) hyaluronic acid capsule that inhibits bacterial internalization and (ii) secreted cytolysin, streptolysin O (SLO), that forms transmembrane pores that permit extracellular calcium influx into the cytosol. (lu.se)
  • Use bioinformatics to explore DNA sequences and protein functions, to find the determinants of virulence in microbes. (wellcomeconnectingscience.org)
  • Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e. (cdc.gov)
  • they inhibit bacterial DNA-dependent RNA polymerase, suppressing RNA synthesis. (msdmanuals.com)
  • by binding to the 50S subunit of the ribosome, they inhibit bacterial protein synthesis. (msdmanuals.com)
  • High specificity and sensitivity for bacterial DNA in a background of non-target DNA. (zymoresearch.com)
  • The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and sensitivity. (zymoresearch.com)
  • During transformation, a bacterium can take up DNA from its environment. (stackexchange.com)
  • With the Femto Bacterial DNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 µl purified from biological liquids, bacterial cultures, or environmental DNA samples. (zymoresearch.com)
  • Research has shown that the ssDNA is stabilized by the interaction of SSB and the χ subunit of DNA polymerase III in E. coli, thus preparing for replication by maintaining the correct conformation that increases the binding affinity of enzymes to ssDNA. (wikipedia.org)
  • The targets of quinolone activity are the bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and transcription. (cdc.gov)
  • Following elution, the protein readily binds DNA, indicating the protein's high affinity for DNA. (wikipedia.org)
  • H-NS binds to DNA with an intrinsic curvature. (wikipedia.org)
  • DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. (qiagen.com)
  • Structural analysis of the C-terminal domain reveals a dimer with a lysine-rich surface that binds DNA non-specifically and is essential for DNA condensation in vitro. (elifesciences.org)
  • The exogenous DNA binds to a receptor located on the surface of competent cells, gets fragmented into small pieces, and then one strand is degraded while the other strand is transported across the membrane into the cytoplasm with the aid of a DNA translocase enzyme. (stackexchange.com)
  • The remaining 106 respiratory samples represented all specimens submitted for investigation of bacterial pneumonia during a one-week period in August 1999, at the Department of Clinical Microbiology, Herlev University Hospital. (biomedcentral.com)
  • Salmonella enterica Quantified Bacterial DNA Standard (Cat. (norgenbiotek.com)
  • Upon receipt, store Norgen's Salmonella enterica Quantified Bacterial DNA Standard at -20 o C or lower. (norgenbiotek.com)
  • Salmonella enterica DNA Standard . (norgenbiotek.com)
  • What does a viral DNA becomes after being associated with the bacterial chromosome? (mcqsmaster.com)
  • DNA gyrase and topoisomerase IV. (cdc.gov)
  • The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation. (elifesciences.org)
  • This study using limited sample size indicated that oral antibiotic therapy may have a limited efficacy on the bacterial population associated with BRONJ lesions. (nih.gov)
  • In collaboration with Stanford University and the University of Hamburg, Solu has developed software to better track, analyse, and manage bacterial genomics. (springwise.com)
  • Although the use of polycation to complex and purify DNA has been described in the literature, elution often requires a high ionic strength or pH levels not compatible with molecular analyses. (mdpi.com)
  • the protein is involved in stabilizing the lagging strand as well as interacting with DNA polymerase III. (wikipedia.org)
  • The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, specifically the interactions between SSB and the χ subunit of DNA polymerase III in environments of varying salt concentrations. (wikipedia.org)
  • Furthermore, binding of SSB to DNA polymerase III at the replication fork prevents dissociation of SSB, consequently increasing the efficiency of DNA polymerase III to synthesize a new DNA strand. (wikipedia.org)
  • When H-NS is bound with RNA Polymerase to the promoter region, there are structural differences in the DNA that are accessible. (wikipedia.org)
  • However, the main limitations of such a tools are that they extract low levels of DNA and they do not remove the inhibitors of polymerase chain reaction (PCR). (mdpi.com)
  • These 'networks' were inferred to be dynamic and poorly-ordered, consisting of several DNA loops between distally bound ParB molecules. (elifesciences.org)
  • Four molecules were chosen to be docked into DNA Gyrase active site using AutoDockTools. (bvsalud.org)
  • The team subsequently observed that two structurally different bacterial toxins also employed this same mechanism. (scitechdaily.com)
  • The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA. (sigmaaldrich.com)
  • Detection of Pneumocystis-DNA was associated with a worse prognosis: seven (44%) of patients with positive PCR died within one month compared to nine (14%) of the controls (p = 0.01). (biomedcentral.com)
  • In order to increase sensitivity in non-invasive specimens, several experimental studies have evaluated PCR detection of P. jiroveci DNA as a potential diagnostic test. (biomedcentral.com)
  • DNA and RNA are ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. (qiagen.com)
  • abstract = "BACKGROUND AND OBJECTIVES: Bacterial-derived DNA fragments (BDNAs) have been shown to be present in dialysis fluid, to pass through dialyzer membranes, and to induce IL-6 (IL-6) in mononuclear cells. (unicatt.it)
  • The bacterial profile from soft tissues associated with the BRONJ lesion was determined using 16S rRNA-based denaturing gradient gel electrophoresis (DGGE) and sequencing. (nih.gov)
  • The estimated residual risk of contamination of blood products with bacterial agents is 1 in 5,000 for platelets and 1 in 30,000 for red blood cells. (medscape.com)
  • Also important is the duration of storage, which has a direct correlation with the likelihood of bacterial contamination. (medscape.com)
  • A meta-analysis of 22 studies of bacterial contamination rate estimates for apheresis (AP), platelet-rich plasma (PRP), and buffy coat (BC) collection methods found an overall mean contamination rate of 0.51 per 1000 components (95% confidence index [CI], 0.38-0.67). (medscape.com)
  • Probable contamination - The blood culture from the recipient is negative or could not be done, but there is definite bacterial growth in the donor blood product. (medscape.com)
  • In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
  • The most common type is the DDE-transposase encoded by most bacterial insertion sequences (IS), eukaryotic DNA transposons, and LTR retrotransposons. (biomedcentral.com)
  • An unstable SSB/DNA system would result in rapid disintegration of the SSB, which stalls DNA replication. (wikipedia.org)
  • Some DNA differences result from the blunders during the DNA replication necessary for cell division. (cdc.gov)
  • RESUME Nous avons étudié la résistance aux antimicrobiens et les profils plasmidiques des isolats d' Escherichia coli uropathogénique chez des malades hospitalisés et des malades externes à l'Hôpital universitaire jordanien en 2000 et 2001. (who.int)
  • To date, there have not been any studies which identify the bacterial presence within healthy, non-abscessed liver tissue. (asas.org)
  • The DGGE fingerprints indicated no significant differences in bacterial diversity of BRONJ tissue samples. (nih.gov)
  • The Bacterial Genomic Dna Extraction Protocol reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (abeurope.info)
  • Respiratory samples from 367 patients suspected of bacterial pneumonia were analysed by PCR amplification of Pneumocystis jiroveci . (biomedcentral.com)
  • The aim of the current study was to evaluate the clinical significance and prevalence of a PCR positive signal in a broad spectrum of clinical samples from HIV-negative patients with suspected bacterial pneumonia by means of an age and sex matched nested case-control study. (biomedcentral.com)
  • The results indicated that the dominant bacterial phyla in the samples were Acidobacteriota , Proteobacteria , Actinobacteriota , Chloroflexi . (researchsquare.com)
  • The bacterial communities' structure of the samples treated with TC, g-C 3 N 4 or TC/g-C 3 N 4 were distinguishable. (researchsquare.com)
  • Clinicians treating these conditions have performed molecular testing of the nails and have been surprised to find clinically significant numbers of bacterial organisms in their samples (see picture appendices 1-2). (nova.edu)
  • Taking into account our results, we recommend Fast as the most suitable DNA extraction method for activated sludge samples used for bacterial community studies. (knaw.nl)
  • Some of the genetic changes alter single DNA building blocks (nucleotides), whereas others rearrange larger segments of mitochondrial DNA. (medlineplus.gov)
  • Thirty percent of bacterial populations were uncultured (yet-to be cultured) phylotypes. (nih.gov)
  • Our objective is designing novel DNA Gyrase inhibitors using Quantitative StructureActivity Relationships and Structure-Based Drug Design Approaches. (bvsalud.org)
  • The 3D crystal structure of DNA Gyrase was extracted from PDB (code: 4DHU) and we characterized the active site. (bvsalud.org)
  • Bacterial Standards are generated from E. coli strain JM109. (zymoresearch.com)
  • Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption. (abeurope.info)
  • Non-LTR retrotransposons need both RT and endonuclease (EN) in their transposition process termed target site-primed reverse transcription (TPRT) [ 12 ]. (biomedcentral.com)
  • Complementary DNA was generated from RNA by reverse transcription with random priming. (uea.ac.uk)
  • These data suggest that modification to CRP, and bound DNA, contributes to UV sensitivity. (ku.dk)
  • Mutations in mitochondrial DNA cause over 150 distinct syndromes and affect 1,000 to 4,000 children born in the United States each year. (sciencenews.org)
  • This genetic material is known as mitochondrial DNA or mtDNA. (medlineplus.gov)
  • In humans, mitochondrial DNA spans about 16,500 DNA building blocks (base pairs), representing a small fraction of the total DNA in cells. (medlineplus.gov)
  • The following chromosomal conditions are associated with changes in the structure or number of copies of mitochondrial dna. (medlineplus.gov)
  • Changes in mitochondrial DNA are among the best-studied genetic factors associated with age-related hearing loss. (medlineplus.gov)
  • As people age, mitochondrial DNA accumulates damaging mutations, including deletions and other changes. (medlineplus.gov)
  • Mitochondrial DNA is especially vulnerable because it has a limited ability to repair itself. (medlineplus.gov)
  • Cells that have high energy demands, such as those in the inner ear that are critical for hearing, are particularly sensitive to the effects of mitochondrial DNA damage. (medlineplus.gov)
  • Some cases of cyclic vomiting syndrome, particularly those that begin in childhood, may be related to changes in mitochondrial DNA. (medlineplus.gov)
  • Bacterial insertion sequences (IS) of IS200 / IS605 and IS607 family often encode a transposase (TnpA) and a protein of unknown function, TnpB. (biomedcentral.com)
  • Then one of those cells mutates through a DNA insertion, deletion, or point mutation, and suddenly a new pattern appears. (cdc.gov)