A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The sum of the weight of all the atoms in a molecule.
Used as a support for ion-exchange chromatography.
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
A cellulose of varied carboxyl content retaining the fibrous structure. It is commonly used as a local hemostatic and as a matrix for normal blood coagulation.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Cellulose derivative used in chromatography, as ion-exchange material, and for various industrial applications.
A species of acetate-oxidizing bacteria, formerly known as Acetobacter xylinum.
Electrophoresis in which cellulose acetate is the diffusion medium.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
High molecular weight, insoluble polymers which contain functional groups that are capable of undergoing exchange reactions (ION EXCHANGE) with either cations or anions.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.
Components of the extracellular matrix consisting primarily of fibrillin. They are essential for the integrity of elastic fibers.
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Polysaccharides consisting of xylose units.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Methylester of cellulose. Methylcellulose is used as an emulsifying and suspending agent in cosmetics, pharmaceutics and the chemical industry. It is used therapeutically as a bulk laxative.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
A species of gram-negative bacteria of the family ACETOBACTERACEAE found in FLOWERS and FRUIT. Cells are ellipsoidal to rod-shaped and straight or slightly curved.
Polysaccharides are complex carbohydrates consisting of long, often branched chains of repeating monosaccharide units joined together by glycosidic bonds, which serve as energy storage molecules (e.g., glycogen), structural components (e.g., cellulose), and molecular recognition sites in various biological systems.
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.
The fruiting 'heads' or 'caps' of FUNGI, which as a food item are familiarly known as MUSHROOMS, that contain the FUNGAL SPORES.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Usually inert substances added to a prescription in order to provide suitable consistency to the dosage form. These include binders, matrix, base or diluent in pills, tablets, creams, salves, etc.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
Glycoside Hydrolases are a class of enzymes that catalyze the hydrolysis of glycosidic bonds, resulting in the breakdown of complex carbohydrates and oligosaccharides into simpler sugars.
The rate dynamics in chemical or physical systems.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Dextrins are a group of partially degraded and digestible starches, formed through the hydrolysis of starch by heat, acids, or enzymes, consisting of shorter chain polymers of D-glucose units linked mainly by α-(1→4) and α-(1→6) glycosidic bonds.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.
Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed)
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A family of bacteria found in the mouth and intestinal and respiratory tracts of man and other animals as well as in the human female urogenital tract. Its organisms are also found in soil and on cereal grains.
The process of cleaving a chemical compound by the addition of a molecule of water.
Dried, ripe seeds of PLANTAGO PSYLLIUM; PLANTAGO INDICA; and PLANTAGO OVATA. Plantain seeds swell in water and are used as demulcents and bulk laxatives.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An exocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages of 1,4-beta-D-glucans resulting in successive removal of GLUCOSE units.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
A genus of gram-positive bacteria in the family Lachnospiraceae that inhabits the RUMEN; LARGE INTESTINE; and CECUM of MAMMALS.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
A genus of gram-negative, anaerobic bacteria in the family Fibrobacteraceae, isolated from the human GASTROINTESTINAL TRACT.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Substances made up of an aggregation of small particles, as that obtained by grinding or trituration of a solid drug. In pharmacy it is a form in which substances are administered. (From Dorland, 28th ed)
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)
A TEXTILE fiber obtained from the pappus (outside the SEEDS) of cotton plant (GOSSYPIUM). Inhalation of cotton fiber dust over a prolonged period can result in BYSSINOSIS.
Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A key intermediate in carbohydrate metabolism. Serves as a precursor of glycogen, can be metabolized into UDPgalactose and UDPglucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. Also serves as a precursor of sucrose lipopolysaccharides, and glycosphingolipids.
Tetroses are uncommon sugars (monosaccharides) with four carbon atoms, having an aldehyde functional group at the first carbon atom, and forming ring structures in their cyclic forms, primarily found in complex carbohydrates and certain natural products.

Unsaturated fatty acid-activated protein kinase (PKx) from goat testis cytosol. (1/28)

The cytosolic fraction of goat cauda epididymis possesses a protein kinase (PKx) activity which is stimulated by a number of unsaturated fatty acids of which arachidonic acid is the best activator in absence of cAMP or Ca(2+). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and diacylglycerol have no effect either alone or in combination. The membrane fraction does not show any appreciable kinase activity even after detergent treatment. PKx migrates as a single band of apparent molecular mass of 116 kDa on 10% SDS-PAGE after sequential chromatographic separation on DEAE-cellulose, phenyl-Sepharose, high-Q anion exchange and protamine-agarose affinity column. PKx phosphorylates histone H1, histone IIIs and protamine sulfate, but not casein. However, the best phosphorylation was obtained with a substrate based on PKC pseudosubstrate sequence (RFARKGSLRQKNV). The kinase phosphorylates two endogenous cytosolic proteins of 60 and 68 kDa. Ser residues are primarily phosphorylated although a low level of phosphorylation is observed on Thr residues also. Ca(2+) and Mn(2+) inhibit PKx activity in the micromolar range. Staurosporine is found to inhibit the PKx activity to a significant level at sub-nanomolar concentration. Lyso-phosphatidylcholine and certain detergents at very low concentrations (<0.05%) stimulate enzyme activity to some extent. The immuno-crossreactivity study with antibody against different PKC isotypes suggests that the protein kinase under study is not related to any known PKC family. Even the antibody against PKN (a related protein kinase reported in rat testis found to be activated by arachidonic acid) does not cross-react with this protein kinase. Hence we believe that the protein kinase (PKx) reported here is different even from the PKN of rat testis. The phosphorylation of endogenous proteins by the protein kinase may be involved in cell regulation including fertility regulation and signal transduction.  (+info)

An easy cell-free protein synthesis system dependent on the addition of crude Escherichia coli tRNA. (2/28)

The protein-synthesizing S30 extract of Escherichia coli contains tRNA, which limits its applications in cell-free protein synthesis. Here, we show that at least Arg- and Ser-acceptor activities can be removed from a standard S30 extract by treatment with an immobilized RNase A resin. This RNase-treated extract exhibits no protein synthesis activity, but regains it when supplied with crude E. coli tRNA and a small amount of human placental RNase inhibitor. The protein synthesis is dependent on the addition of tRNA in the presence of the RNase inhibitor. Chloramphenicol acetyltransferase was synthesized with this system and found to be active.  (+info)

A simplified method for study of RNA conformation--reaction of formylmethionine transfer RNA with [14C]methylamine-bisulfite. (3/28)

A new chemical method for radioactive labeling of single-stranded regions of RNA has been used to probe the three-dimensional structure of E. coli tRNA(fMet) in solution. The procedure involves conversion of cytosine residues to N(4)-[(14)C]methylcytosines by treatment with (14)CH(3)NH(2) and sodium bisulfite at pH7. Ribonuclease digestion of the modified tRNA produces (14)C-labeled oligonucleotides which comigrate with the corresponding unlabeled oligonucleotides, facilitating structural analysis. By this procedure, E. coli tRNA(fMet) has been found to contain only six reactive cytosines: C(1), C(16), C(17), C(35), C(75) and C(76). In addition, slow reaction at C(m) (33) was observed. These results are in excellent agreement with previously reported data on the sites of exposed cytosine residues in tRNA(fMet) obtained by two other chemical methods. The methylamine-bisulfite procedure is recommended for studying the ordered structure of more complex polyribonucleotides such as viral and ribosomal RNAs.  (+info)

DNA sequence analysis: a formula to predict electrophoretic mobilities of oligonucleotides on cellulose acetate. (4/28)

A simple method has recently become available for sequence analysis of large oligonucleotide fragments. Sequences are derived from the characteristic mobility shifts of the sequential partial degradation products of the oligonucleotide on two dimensional homochromatography. We have now developed an empirical formula for predicting the relative mobilities of each of the partial products in the first dimension (electrophoresis on cellulose acetate gel). The formula allows a more precise interpretation of the sequence of the oligonucleotide. It eliminates the ambiguities present in the method previously reported for sequence analysis by simple inspection of the mobility shifts. In order to amplify the mobility shifts so that they may be more easily and accurately measured, methods have been developed for preparing and fractionating the oligonucleotides on 40 x 40 cm DEAE-cellulose plates. Both improvements have proven valuable for direct sequence analysis by mapping.  (+info)

Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E. coli phenylalanyl-tRNA(1Val) on BD-cellulose. (5/28)

The chromatographic behaviour of E.coli tRNA(Val) (1), Val-tRNA(Val) (1) and Phe-tRNA(Val) (1) was studied on BD-cellulose columns. At pH 4.0 and 4 degrees C the elution position of Phe-tRNA(Val) (1) was not affected by the presence of absence of Mg(2+), whereas Val-tRNA(Val) (1) was slightly retarded when Mg(2+) was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA(Val) (1) eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.  (+info)

Purification of soluble beta-glucan with immune-enhancing activity from the cell wall of yeast. (6/28)

Beta-glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of beta-(1-->3)-glucan is its low solubility in aqueous media. In this study, soluble beta-glucan, free of mannoprotein, was prepared, and its effects on TNF-alpha secretion and phagocytosis by macrophages were evaluated. Beta-glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. Beta-glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of beta-glucan on phagocytosis and TNF-alpha release activity were investigated. While glucan-p1 moderately induced TNF-alpha secretion at 200 microg/ml (550 pg of TNF-alpha/5 x 10(5) cells), glucan-p3 markedly stimulated macrophages at 200 microg/ml (2,860 pg of TNF-alpha/5 x 10(5) cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified water-soluble beta-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.  (+info)

Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm. (7/28)

Polymyxin-resistant mutants of Escherichia coli and Salmonella typhimurium accumulate a novel minor lipid that can donate 4-amino-4-deoxy-l-arabinose units (l-Ara4N) to lipid A. We now report the purification of this lipid from a pss(-) pmrA(C) mutant of E. coli and assign its structure as undecaprenyl phosphate-alpha-l-Ara4N. Approximately 0.2 mg of homogeneous material was isolated from an 8-liter culture by solvent extraction, followed by chromatography on DEAE-cellulose, C18 reverse phase resin, and silicic acid. Matrix-assisted laser desorption ionization/time of flight mass spectrometry in the negative mode yielded a single species [M - H](-) at m/z 977.5, consistent with undecaprenyl phosphate-alpha-l-Ara4N (M(r) = 978.41). (31)P NMR spectroscopy showed a single phosphorus atom at -0.44 ppm characteristic of a phosphodiester linkage. Selective inverse decoupling difference spectroscopy demonstrated that the undecaprenyl phosphate group is attached to the anomeric carbon of the l-Ara4N unit. One- and two-dimensional (1)H NMR studies confirmed the presence of a polyisoprene chain and a sugar moiety with chemical shifts and coupling constants expected for an equatorially substituted arabinopyranoside. Heteronuclear multiple-quantum coherence spectroscopy analysis demonstrated that a nitrogen atom is attached to C-4 of the sugar residue. The purified donor supports in vitro conversion of lipid IV(A) to lipid II(A), which is substituted with a single l-Ara4N moiety. The identification of undecaprenyl phosphate-alpha-l-Ara4N implies that l-Ara4N transfer to lipid A occurs in the periplasm of polymyxin-resistant strains, and establishes a new enzymatic pathway by which Gram-negative bacteria acquire antibiotic resistance.  (+info)

Prothrombin complex concentrates: potentially thrombogenic materials and clues to the mechanism of thrombosis in vivo. (8/28)

Factors affecting the coagulant activity of two different prothrombin complex concentrates have been investigated using a sensitive in vitro assay developed in this laboratory. One concentrate contained substantial amounts of potentially thrombogenic material, while the other, which was deliberately fortified with antithrombin III and heparin during production, was judged to be relatively nonthrombogenic. The coagulant activity of the thrombogenic concentrate has been partially identified and was due largely to the presence of coagulation factos IXa and Xa. Neither concentrate contained detectable thrombin. However, after incubation with calcium or various polyamines, large amounts of additional coagulant material, including thrombin, appeared. Heparin and antithrombin III not only neutralized the thrombogenic materials present in the thrombogenic concentrate, but also inhibited the de novo generation of coagulant enzymes during incubation with calcium. The implication of these studies on the preparation of prothrombin complex concentrates and on host susceptibility to thrombosis during the clinical use of these concentrates is discussed.  (+info)

Cellulose is a complex carbohydrate that is the main structural component of the cell walls of green plants, many algae, and some fungi. It is a polysaccharide consisting of long chains of beta-glucose molecules linked together by beta-1,4 glycosidic bonds. Cellulose is insoluble in water and most organic solvents, and it is resistant to digestion by humans and non-ruminant animals due to the lack of cellulase enzymes in their digestive systems. However, ruminants such as cows and sheep can digest cellulose with the help of microbes in their rumen that produce cellulase.

Cellulose has many industrial applications, including the production of paper, textiles, and building materials. It is also used as a source of dietary fiber in human food and animal feed. Cellulose-based materials are being explored for use in biomedical applications such as tissue engineering and drug delivery due to their biocompatibility and mechanical properties.

DEAE-cellulose chromatography is a method of purification and separation of biological molecules such as proteins, nucleic acids, and enzymes. DEAE stands for diethylaminoethyl, which is a type of charged functional group that is covalently bound to cellulose, creating a matrix with positive charges.

In this method, the mixture of biological molecules is applied to a column packed with DEAE-cellulose. The positively charged DEAE groups attract and bind negatively charged molecules in the mixture, such as nucleic acids and proteins, while allowing uncharged or neutrally charged molecules to pass through.

By adjusting the pH, ionic strength, or concentration of salt in the buffer solution used to elute the bound molecules from the column, it is possible to selectively elute specific molecules based on their charge and binding affinity to the DEAE-cellulose matrix. This makes DEAE-cellulose chromatography a powerful tool for purifying and separating biological molecules with high resolution and efficiency.

Chromatography is a technique used in analytical chemistry for the separation, identification, and quantification of the components of a mixture. It is based on the differential distribution of the components of a mixture between a stationary phase and a mobile phase. The stationary phase can be a solid or liquid, while the mobile phase is a gas, liquid, or supercritical fluid that moves through the stationary phase carrying the sample components.

The interaction between the sample components and the stationary and mobile phases determines how quickly each component will move through the system. Components that interact more strongly with the stationary phase will move more slowly than those that interact more strongly with the mobile phase. This difference in migration rates allows for the separation of the components, which can then be detected and quantified.

There are many different types of chromatography, including paper chromatography, thin-layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC), and high-performance liquid chromatography (HPLC). Each type has its own strengths and weaknesses, and is best suited for specific applications.

In summary, chromatography is a powerful analytical technique used to separate, identify, and quantify the components of a mixture based on their differential distribution between a stationary phase and a mobile phase.

Ion exchange chromatography is a type of chromatography technique used to separate and analyze charged molecules (ions) based on their ability to exchange bound ions in a solid resin or gel with ions of similar charge in the mobile phase. The stationary phase, often called an ion exchanger, contains fixed ated functional groups that can attract counter-ions of opposite charge from the sample mixture.

In this technique, the sample is loaded onto an ion exchange column containing the charged resin or gel. As the sample moves through the column, ions in the sample compete for binding sites on the stationary phase with ions already present in the column. The ions that bind most strongly to the stationary phase will elute (come off) slower than those that bind more weakly.

Ion exchange chromatography can be performed using either cation exchangers, which exchange positive ions (cations), or anion exchangers, which exchange negative ions (anions). The pH and ionic strength of the mobile phase can be adjusted to control the binding and elution of specific ions.

Ion exchange chromatography is widely used in various applications such as water treatment, protein purification, and chemical analysis.

Gel chromatography is a type of liquid chromatography that separates molecules based on their size or molecular weight. It uses a stationary phase that consists of a gel matrix made up of cross-linked polymers, such as dextran, agarose, or polyacrylamide. The gel matrix contains pores of various sizes, which allow smaller molecules to penetrate deeper into the matrix while larger molecules are excluded.

In gel chromatography, a mixture of molecules is loaded onto the top of the gel column and eluted with a solvent that moves down the column by gravity or pressure. As the sample components move down the column, they interact with the gel matrix and get separated based on their size. Smaller molecules can enter the pores of the gel and take longer to elute, while larger molecules are excluded from the pores and elute more quickly.

Gel chromatography is commonly used to separate and purify proteins, nucleic acids, and other biomolecules based on their size and molecular weight. It is also used in the analysis of polymers, colloids, and other materials with a wide range of applications in chemistry, biology, and medicine.

Molecular weight, also known as molecular mass, is the mass of a molecule. It is expressed in units of atomic mass units (amu) or daltons (Da). Molecular weight is calculated by adding up the atomic weights of each atom in a molecule. It is a useful property in chemistry and biology, as it can be used to determine the concentration of a substance in a solution, or to calculate the amount of a substance that will react with another in a chemical reaction.

DEAE-Dextran is a water-soluble polymer that is often used in biochemistry and molecular biology research. The acronym "DEAE" stands for diethylaminoethyl, which is a type of charged group that can bind to and interact with negatively charged molecules such as DNA. Dextran is a type of sugar polymer that makes the DEAE groups more soluble in water.

In research settings, DEAE-Dextran is commonly used to precipitate DNA or to create complexes with DNA that can be used for various purposes, such as transfection (the process of introducing genetic material into cells). The positive charge of the DEAE groups allows them to interact strongly with the negative charges on the DNA molecule, forming a stable complex that can be taken up by cells.

It's important to note that DEAE-Dextran is not used in clinical medicine, but rather as a research tool in laboratory settings.

Cellulose 1,4-beta-Cellobiosidase is an enzyme that catalyzes the hydrolysis of cellulose, a complex carbohydrate and the main structural component of plant cell walls, into simpler sugars. Specifically, this enzyme breaks down cellulose by cleaving the 1,4-beta-glycosidic bonds between the cellobiose units that make up the cellulose polymer, releasing individual cellobiose molecules (disaccharides consisting of two glucose molecules). This enzyme is also known as cellobiohydrolase or beta-1,4-D-glucan cellobiohydrolase. It plays a crucial role in the natural breakdown of plant material and is widely used in various industrial applications, such as biofuel production and pulp and paper manufacturing.

Oxidized cellulose is a type of modified cellulose that has undergone oxidation, resulting in the introduction of functional groups such as carboxylic acid or aldehyde groups along the cellulose chain. This process can alter the physical and chemical properties of cellulose, making it more soluble in water and capable of forming gels or films.

Oxidized cellulose is used in a variety of applications, including as a wound dressing material, where it can help to promote healing by providing a moist environment that supports tissue regeneration. It can also be used as a thickening or stabilizing agent in food and cosmetic products, or as a component in the manufacture of specialized papers and textiles.

Cellulase is a type of enzyme that breaks down cellulose, which is a complex carbohydrate and the main structural component of plant cell walls. Cellulases are produced by certain bacteria, fungi, and protozoans, and are used in various industrial applications such as biofuel production, food processing, and textile manufacturing. In the human body, there are no known physiological roles for cellulases, as humans do not produce these enzymes and cannot digest cellulose.

DEAE-cellulose is a type of ion exchange chromatography material that is commonly used in biochemistry and molecular biology research. DEAE stands for diethylaminoethyl, which is a positively charged functional group that is covalently attached to cellulose, a negatively charged polysaccharide.

The positive charges on the DEAE-cellulose bind to negatively charged molecules such as DNA, RNA, and proteins, allowing for their separation and purification based on differences in charge and binding affinity. The bound molecules can then be eluted or washed off the column using a buffer with increasing ionic strength or changing pH, which weakens the interaction between the DEAE-cellulose and the target molecule.

DEAE-cellulose is a versatile and widely used chromatography material that has been instrumental in many scientific discoveries and advances in molecular biology.

*Gluconacetobacter xylinus*, also known as *Acetobacter xylinum*, is a gram-negative, acetic acid-producing bacterium that is commonly found in fermenting fruits, vegetables, and other plant materials. It is an obligate aerobe, which means it requires oxygen to grow. This bacterium is well-known for its ability to produce cellulose, a complex carbohydrate, as a major component of its extracellular matrix. The cellulose produced by *G. xylinus* is pure and highly crystalline, making it an attractive material for various industrial applications, including the production of biodegradable plastics, nanocomposites, and medical materials. In the medical field, the cellulose produced by this bacterium has been studied for its potential use in wound healing, tissue engineering, and drug delivery systems.

Electrophoresis, cellulose acetate is a laboratory technique used to separate and analyze proteins or other charged molecules based on their size and charge. The sample is applied to a sheet of cellulose acetate, a type of porous plastic film, and an electric field is applied. The proteins migrate through the film towards the electrode with the opposite charge, with smaller and more negatively charged molecules moving faster than larger and less negatively charged ones. This allows for the separation and identification of different protein components in a mixture. It is a simple and rapid method for routine protein separations and is commonly used in biochemistry and molecular biology research.

Carboxymethylcellulose sodium is a type of cellulose derivative that is widely used in the medical and pharmaceutical fields as an excipient or a drug delivery agent. It is a white, odorless powder with good water solubility and forms a clear, viscous solution.

Chemically, carboxymethylcellulose sodium is produced by reacting cellulose, which is derived from plant sources such as wood or cotton, with sodium hydroxide and chloroacetic acid. This reaction introduces carboxymethyl groups (-CH2COO-) to the cellulose molecule, making it more soluble in water and providing negative charges that can interact with positively charged ions or drugs.

In medical applications, carboxymethylcellulose sodium is used as a thickening agent, binder, disintegrant, and suspending agent in various pharmaceutical formulations such as tablets, capsules, liquids, and semisolids. It can also be used as a lubricant in the manufacture of tablets and capsules to facilitate their ejection from molds or dies.

Carboxymethylcellulose sodium has been shown to have good biocompatibility and low toxicity, making it a safe and effective excipient for use in medical and pharmaceutical applications. However, like any other excipient, it should be used with caution and in appropriate amounts to avoid any adverse effects or interactions with the active ingredients of the drug product.

Cellulases are a group of enzymes that break down cellulose, which is a complex carbohydrate and the main structural component of plant cell walls. These enzymes are produced by various organisms, including bacteria, fungi, and protozoa. They play an important role in the natural decomposition process and have various industrial applications, such as in the production of biofuels, paper, and textiles.

Cellulases work by hydrolyzing the beta-1,4 glycosidic bonds between the glucose molecules that make up cellulose, breaking it down into simpler sugars like glucose. This process is known as saccharification. The specific type of cellulase enzyme determines where on the cellulose molecule it will cleave the bond.

There are three main types of cellulases: endoglucanases, exoglucanases, and beta-glucosidases. Endoglucanases randomly attack internal bonds in the amorphous regions of cellulose, creating new chain ends for exoglucanases to act on. Exoglucanases (also known as cellobiohydrolases) cleave cellobiose units from the ends of the cellulose chains, releasing cellobiose or glucose. Beta-glucosidases convert cellobiose into two molecules of glucose, which can then be further metabolized by the organism.

In summary, cellulases are a group of enzymes that break down cellulose into simpler sugars through hydrolysis. They have various industrial applications and play an essential role in natural decomposition processes.

Cellobiose is a disaccharide made up of two molecules of glucose joined by a β-1,4-glycosidic bond. It is formed when cellulose or beta-glucans are hydrolyzed, and it can be further broken down into its component glucose molecules by the action of the enzyme beta-glucosidase. Cellobiose has a sweet taste, but it is not as sweet as sucrose (table sugar). It is used in some industrial processes and may have potential applications in the food industry.

Glucosyltransferases (GTs) are a group of enzymes that catalyze the transfer of a glucose molecule from an activated donor to an acceptor molecule, resulting in the formation of a glycosidic bond. These enzymes play crucial roles in various biological processes, including the biosynthesis of complex carbohydrates, cell wall synthesis, and protein glycosylation. In some cases, GTs can also contribute to bacterial pathogenesis by facilitating the attachment of bacteria to host tissues through the formation of glucans, which are polymers of glucose molecules.

GTs can be classified into several families based on their sequence similarities and catalytic mechanisms. The donor substrates for GTs are typically activated sugars such as UDP-glucose, TDP-glucose, or GDP-glucose, which serve as the source of the glucose moiety that is transferred to the acceptor molecule. The acceptor can be a wide range of molecules, including other sugars, proteins, lipids, or small molecules.

In the context of human health and disease, GTs have been implicated in various pathological conditions, such as cancer, inflammation, and microbial infections. For example, some GTs can modify proteins on the surface of cancer cells, leading to increased cell proliferation, migration, and invasion. Additionally, GTs can contribute to bacterial resistance to antibiotics by modifying the structure of bacterial cell walls or by producing biofilms that protect bacteria from host immune responses and antimicrobial agents.

Overall, Glucosyltransferases are essential enzymes involved in various biological processes, and their dysregulation has been associated with several human diseases. Therefore, understanding the structure, function, and regulation of GTs is crucial for developing novel therapeutic strategies to target these enzymes and treat related pathological conditions.

Ion exchange resins are insoluble, cross-linked polymeric materials that contain functional groups which can exchange ions with surrounding solutions. These resins are typically used in water treatment and purification processes to remove unwanted dissolved ions, molecules, or gases. They operate through the principle of ion exchange, where ions held on the resin are exchanged for ions in the solution. The process can be used to soften water, remove heavy metals, treat wastewater, and deionize water, among other applications.

The resins consist of a three-dimensional network of cross-linked polymer chains, providing a large surface area for ion exchange. They are often made from styrene and divinylbenzene monomers, which form a rigid structure that can withstand repeated ion exchange cycles without losing its shape or functionality. The functional groups on the resins can be cationic (positively charged) or anionic (negatively charged), allowing them to attract and retain ions of opposite charge from the surrounding solution.

Cation exchange resins are used to remove positively charged ions, such as calcium, magnesium, sodium, and potassium, while anion exchange resins are used to remove negatively charged ions, such as chloride, sulfate, nitrate, and bicarbonate. The resins can be regenerated by washing them with a strong solution of the ion to be recovered, allowing them to be reused multiple times before they need to be replaced.

Dextrans are a type of complex glucose polymers that are formed by the action of certain bacteria on sucrose. They are branched polysaccharides consisting of linear chains of α-1,6 linked D-glucopyranosyl units with occasional α-1,3 branches.

Dextrans have a wide range of applications in medicine and industry. In medicine, dextrans are used as plasma substitutes, volume expanders, and anticoagulants. They are also used as carriers for drugs and diagnostic agents, and in the manufacture of immunoadsorbents for the removal of toxins and pathogens from blood.

Dextrans can be derived from various bacterial sources, but the most common commercial source is Leuconostoc mesenteroides B-512(F) or L. dextranicum. The molecular weight of dextrans can vary widely, ranging from a few thousand to several million Daltons, depending on the method of preparation and purification.

Dextrans are generally biocompatible and non-toxic, but they can cause allergic reactions in some individuals. Therefore, their use as medical products requires careful monitoring and testing for safety and efficacy.

Electrophoresis, polyacrylamide gel (EPG) is a laboratory technique used to separate and analyze complex mixtures of proteins or nucleic acids (DNA or RNA) based on their size and electrical charge. This technique utilizes a matrix made of cross-linked polyacrylamide, a type of gel, which provides a stable and uniform environment for the separation of molecules.

In this process:

1. The polyacrylamide gel is prepared by mixing acrylamide monomers with a cross-linking agent (bis-acrylamide) and a catalyst (ammonium persulfate) in the presence of a buffer solution.
2. The gel is then poured into a mold and allowed to polymerize, forming a solid matrix with uniform pore sizes that depend on the concentration of acrylamide used. Higher concentrations result in smaller pores, providing better resolution for separating smaller molecules.
3. Once the gel has set, it is placed in an electrophoresis apparatus containing a buffer solution. Samples containing the mixture of proteins or nucleic acids are loaded into wells on the top of the gel.
4. An electric field is applied across the gel, causing the negatively charged molecules to migrate towards the positive electrode (anode) while positively charged molecules move toward the negative electrode (cathode). The rate of migration depends on the size, charge, and shape of the molecules.
5. Smaller molecules move faster through the gel matrix and will migrate farther from the origin compared to larger molecules, resulting in separation based on size. Proteins and nucleic acids can be selectively stained after electrophoresis to visualize the separated bands.

EPG is widely used in various research fields, including molecular biology, genetics, proteomics, and forensic science, for applications such as protein characterization, DNA fragment analysis, cloning, mutation detection, and quality control of nucleic acid or protein samples.

Trichoderma is a genus of fungi that are commonly found in soil, decaying wood, and other organic matter. While there are many different species of Trichoderma, some of them have been studied for their potential use in various medical and industrial applications. For example, certain Trichoderma species have been shown to have antimicrobial properties and can be used to control plant diseases. Other species are being investigated for their ability to produce enzymes and other compounds that may have industrial or medicinal uses.

However, it's important to note that not all Trichoderma species are beneficial, and some of them can cause infections in humans, particularly in individuals with weakened immune systems. These infections can be difficult to diagnose and treat, as they often involve multiple organ systems and may require aggressive antifungal therapy.

In summary, Trichoderma is a genus of fungi that can have both beneficial and harmful effects on human health, depending on the specific species involved and the context in which they are encountered.

Microfibrils are tiny, thread-like structures that are found in the extracellular matrix (the material that surrounds and supports cells) of many types of biological tissues. They are made up of bundles of long, thin proteins called fibrillins, which are joined together by other proteins such as microfibril-associated glycoproteins (MAGPs).

Microfibrils play an important role in providing structural support and elasticity to tissues. They are particularly abundant in the connective tissue that surrounds blood vessels, where they help to regulate the diameter of the vessels and maintain blood pressure. Microfibrils are also found in the elastic fibers of the lungs, skin, and other tissues, where they contribute to the ability of these tissues to stretch and recoil.

In addition to their structural roles, microfibrils have been shown to play a role in regulating cell behavior and signaling. For example, they can bind to growth factors and other signaling molecules, helping to control the activity of these molecules and influence cellular processes such as proliferation, differentiation, and migration.

Abnormalities in microfibril structure or function have been linked to a number of diseases, including Marfan syndrome, Loeys-Dietz syndrome, and cutis laxa. These conditions are characterized by problems with connective tissue strength and elasticity, which can lead to a range of symptoms such as skeletal abnormalities, cardiovascular disease, and skin fragility.

Pectins are complex polysaccharides that are commonly found in the cell walls of plants. In the context of food and nutrition, pectins are often referred to as dietary fiber. They have a variety of important functions within the body, including promoting digestive health by adding bulk to stools and helping to regulate bowel movements.

Pectins are also used in the medical field as a demulcent, which is a substance that forms a soothing film over mucous membranes. This can be helpful in treating conditions such as gastroesophageal reflux disease (GERD) and inflammatory bowel disease (IBD).

In addition to their use in medicine, pectins are widely used in the food industry as a gelling agent, thickener, and stabilizer. They are commonly found in jams, jellies, and other preserved fruits, as well as in baked goods and confectionery products.

Xylans are a type of complex carbohydrate, specifically a hemicellulose, that are found in the cell walls of many plants. They are made up of a backbone of beta-1,4-linked xylose sugar molecules and can be substituted with various side groups such as arabinose, glucuronic acid, and acetyl groups. Xylans are indigestible by humans, but they can be broken down by certain microorganisms in the gut through a process called fermentation, which can produce short-chain fatty acids that have beneficial effects on health.

Hydrogen-ion concentration, also known as pH, is a measure of the acidity or basicity of a solution. It is defined as the negative logarithm (to the base 10) of the hydrogen ion activity in a solution. The standard unit of measurement is the pH unit. A pH of 7 is neutral, less than 7 is acidic, and greater than 7 is basic.

In medical terms, hydrogen-ion concentration is important for maintaining homeostasis within the body. For example, in the stomach, a high hydrogen-ion concentration (low pH) is necessary for the digestion of food. However, in other parts of the body such as blood, a high hydrogen-ion concentration can be harmful and lead to acidosis. Conversely, a low hydrogen-ion concentration (high pH) in the blood can lead to alkalosis. Both acidosis and alkalosis can have serious consequences on various organ systems if not corrected.

I'm sorry for any confusion, but "Lignin" is not a medical term. It is a term used in the field of biology and chemistry, particularly in botany and wood science. Lignin is a complex organic polymer that binds cellulose fibers together, providing strength and rigidity to the cell walls of plants. It is a major component of wood and bark.

If you have any medical terms you would like defined or any other questions, please let me know!

Methylcellulose is a semisynthetic, inert, viscous, and tasteless white powder that is soluble in cold water but not in hot water. It is derived from cellulose through the process of methylation. In medical contexts, it is commonly used as a bulk-forming laxative to treat constipation, as well as a lubricant in ophthalmic solutions and a suspending agent in pharmaceuticals.

When mixed with water, methylcellulose forms a gel-like substance that can increase stool volume and promote bowel movements. It is generally considered safe for most individuals, but like any medication or supplement, it should be used under the guidance of a healthcare provider.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

A cell wall is a rigid layer found surrounding the plasma membrane of plant cells, fungi, and many types of bacteria. It provides structural support and protection to the cell, maintains cell shape, and acts as a barrier against external factors such as chemicals and mechanical stress. The composition of the cell wall varies among different species; for example, in plants, it is primarily made up of cellulose, hemicellulose, and pectin, while in bacteria, it is composed of peptidoglycan.

'Acetobacter' is a genus of gram-negative, aerobic, rod-shaped bacteria that are commonly found in various environments such as soil, water, and plant surfaces. They are known for their ability to oxidize alcohols to aldehydes and then to carboxylic acids, particularly the oxidation of ethanol to acetic acid. This property makes them important in the production of vinegar and other fermented foods. Some species of Acetobacter can also cause food spoilage and may be associated with certain human infections, although they are not considered primary human pathogens.

Polysaccharides are complex carbohydrates consisting of long chains of monosaccharide units (simple sugars) bonded together by glycosidic linkages. They can be classified based on the type of monosaccharides and the nature of the bonds that connect them.

Polysaccharides have various functions in living organisms. For example, starch and glycogen serve as energy storage molecules in plants and animals, respectively. Cellulose provides structural support in plants, while chitin is a key component of fungal cell walls and arthropod exoskeletons.

Some polysaccharides also have important roles in the human body, such as being part of the extracellular matrix (e.g., hyaluronic acid) or acting as blood group antigens (e.g., ABO blood group substances).

'Clostridium thermocellum' is a type of anaerobic, gram-positive bacterium that is known for its ability to produce cellulases and break down cellulose. It is thermophilic, meaning it grows optimally at higher temperatures, typically between 55-70°C. This organism is of interest in the field of bioenergy because of its potential to convert plant biomass into useful products such as biofuels. However, it's important to note that this bacterium can also produce harmful metabolic byproducts and can be potentially pathogenic to humans.

Dietary fiber, also known as roughage, is the indigestible portion of plant foods that makes up the structural framework of the plants we eat. It is composed of cellulose, hemicellulose, pectin, gums, lignins, and waxes. Dietary fiber can be classified into two categories: soluble and insoluble.

Soluble fiber dissolves in water to form a gel-like material in the gut, which can help slow down digestion, increase feelings of fullness, and lower cholesterol levels. Soluble fiber is found in foods such as oats, barley, fruits, vegetables, legumes, and nuts.

Insoluble fiber does not dissolve in water and passes through the gut intact, helping to add bulk to stools and promote regular bowel movements. Insoluble fiber is found in foods such as whole grains, bran, seeds, and the skins of fruits and vegetables.

Dietary fiber has numerous health benefits, including promoting healthy digestion, preventing constipation, reducing the risk of heart disease, controlling blood sugar levels, and aiding in weight management. The recommended daily intake of dietary fiber is 25-38 grams per day for adults, depending on age and gender.

A fruiting body, in the context of mycology (the study of fungi), refers to the part of a fungus that produces spores for sexual or asexual reproduction. These structures are often what we typically think of as mushrooms or toadstools, although not all fungal fruiting bodies resemble these familiar forms.

Fungal fruiting bodies can vary greatly in size, shape, and color, depending on the species of fungus. They may be aboveground, like the caps and stalks of mushrooms, or underground, like the tiny, thread-like structures known as "corals" in some species.

The primary function of a fruiting body is to produce and disperse spores, which can give rise to new individuals when they germinate under favorable conditions. The development of a fruiting body is often triggered by environmental factors such as moisture, temperature, and nutrient availability.

Substrate specificity in the context of medical biochemistry and enzymology refers to the ability of an enzyme to selectively bind and catalyze a chemical reaction with a particular substrate (or a group of similar substrates) while discriminating against other molecules that are not substrates. This specificity arises from the three-dimensional structure of the enzyme, which has evolved to match the shape, charge distribution, and functional groups of its physiological substrate(s).

Substrate specificity is a fundamental property of enzymes that enables them to carry out highly selective chemical transformations in the complex cellular environment. The active site of an enzyme, where the catalysis takes place, has a unique conformation that complements the shape and charge distribution of its substrate(s). This ensures efficient recognition, binding, and conversion of the substrate into the desired product while minimizing unwanted side reactions with other molecules.

Substrate specificity can be categorized as:

1. Absolute specificity: An enzyme that can only act on a single substrate or a very narrow group of structurally related substrates, showing no activity towards any other molecule.
2. Group specificity: An enzyme that prefers to act on a particular functional group or class of compounds but can still accommodate minor structural variations within the substrate.
3. Broad or promiscuous specificity: An enzyme that can act on a wide range of structurally diverse substrates, albeit with varying catalytic efficiencies.

Understanding substrate specificity is crucial for elucidating enzymatic mechanisms, designing drugs that target specific enzymes or pathways, and developing biotechnological applications that rely on the controlled manipulation of enzyme activities.

Beta-glucosidase is an enzyme that breaks down certain types of complex sugars, specifically those that contain a beta-glycosidic bond. This enzyme is found in various organisms, including humans, and plays a role in the digestion of some carbohydrates, such as cellulose and other plant-based materials.

In the human body, beta-glucosidase is produced by the lysosomes, which are membrane-bound organelles found within cells that help break down and recycle various biological molecules. Beta-glucosidase is involved in the breakdown of glycolipids and gangliosides, which are complex lipids that contain sugar molecules.

Deficiencies in beta-glucosidase activity can lead to certain genetic disorders, such as Gaucher disease, in which there is an accumulation of glucocerebrosidase, a type of glycolipid, within the lysosomes. This can result in various symptoms, including enlargement of the liver and spleen, anemia, and bone pain.

An amino acid sequence is the specific order of amino acids in a protein or peptide molecule, formed by the linking of the amino group (-NH2) of one amino acid to the carboxyl group (-COOH) of another amino acid through a peptide bond. The sequence is determined by the genetic code and is unique to each type of protein or peptide. It plays a crucial role in determining the three-dimensional structure and function of proteins.

Excipients are inactive substances that serve as vehicles or mediums for the active ingredients in medications. They make up the bulk of a pharmaceutical formulation and help to stabilize, preserve, and enhance the delivery of the active drug compound. Common examples of excipients include binders, fillers, coatings, disintegrants, flavors, sweeteners, and colors. While excipients are generally considered safe and inert, they can sometimes cause allergic reactions or other adverse effects in certain individuals.

'Clostridium' is a genus of gram-positive, rod-shaped bacteria that are widely distributed in nature, including in soil, water, and the gastrointestinal tracts of animals and humans. Many species of Clostridium are anaerobic, meaning they can grow and reproduce in environments with little or no oxygen. Some species of Clostridium are capable of producing toxins that can cause serious and sometimes life-threatening illnesses in humans and animals.

Some notable species of Clostridium include:

* Clostridium tetani, which causes tetanus (also known as lockjaw)
* Clostridium botulinum, which produces botulinum toxin, the most potent neurotoxin known and the cause of botulism
* Clostridium difficile, which can cause severe diarrhea and colitis, particularly in people who have recently taken antibiotics
* Clostridium perfringens, which can cause food poisoning and gas gangrene.

It is important to note that not all species of Clostridium are harmful, and some are even beneficial, such as those used in the production of certain fermented foods like sauerkraut and natto. However, due to their ability to produce toxins and cause illness, it is important to handle and dispose of materials contaminated with Clostridium species carefully, especially in healthcare settings.

Affinity chromatography is a type of chromatography technique used in biochemistry and molecular biology to separate and purify proteins based on their biological characteristics, such as their ability to bind specifically to certain ligands or molecules. This method utilizes a stationary phase that is coated with a specific ligand (e.g., an antibody, antigen, receptor, or enzyme) that selectively interacts with the target protein in a sample.

The process typically involves the following steps:

1. Preparation of the affinity chromatography column: The stationary phase, usually a solid matrix such as agarose beads or magnetic beads, is modified by covalently attaching the ligand to its surface.
2. Application of the sample: The protein mixture is applied to the top of the affinity chromatography column, allowing it to flow through the stationary phase under gravity or pressure.
3. Binding and washing: As the sample flows through the column, the target protein selectively binds to the ligand on the stationary phase, while other proteins and impurities pass through. The column is then washed with a suitable buffer to remove any unbound proteins and contaminants.
4. Elution of the bound protein: The target protein can be eluted from the column using various methods, such as changing the pH, ionic strength, or polarity of the buffer, or by introducing a competitive ligand that displaces the bound protein.
5. Collection and analysis: The eluted protein fraction is collected and analyzed for purity and identity, often through techniques like SDS-PAGE or mass spectrometry.

Affinity chromatography is a powerful tool in biochemistry and molecular biology due to its high selectivity and specificity, enabling the efficient isolation of target proteins from complex mixtures. However, it requires careful consideration of the binding affinity between the ligand and the protein, as well as optimization of the elution conditions to minimize potential damage or denaturation of the purified protein.

Cellulosomes are large, complex enzymatic structures produced by certain anaerobic bacteria that allow them to break down and consume cellulose, a major component of plant biomass. These structures are composed of multiple enzymes that work together in a coordinated manner to degrade cellulose into simpler sugars, which the bacteria can then use as a source of energy and carbon.

The individual enzymes in a cellulosome are non-covalently associated with a central scaffoldin protein, forming a multi-enzyme complex. The scaffoldin protein contains cohesin modules that bind to dockerin modules on the enzyme subunits, creating a highly organized and stable structure.

Cellulosomes have been identified in several species of anaerobic bacteria, including members of the genera Clostridium and Ruminococcus. They are thought to play a key role in the global carbon cycle by breaking down plant material and releasing carbon dioxide back into the atmosphere.

Glycoside hydrolases are a class of enzymes that catalyze the hydrolysis of glycosidic bonds found in various substrates such as polysaccharides, oligosaccharides, and glycoproteins. These enzymes break down complex carbohydrates into simpler sugars by cleaving the glycosidic linkages that connect monosaccharide units.

Glycoside hydrolases are classified based on their mechanism of action and the type of glycosidic bond they hydrolyze. The classification system is maintained by the International Union of Biochemistry and Molecular Biology (IUBMB). Each enzyme in this class is assigned a unique Enzyme Commission (EC) number, which reflects its specificity towards the substrate and the type of reaction it catalyzes.

These enzymes have various applications in different industries, including food processing, biofuel production, pulp and paper manufacturing, and biomedical research. In medicine, glycoside hydrolases are used to diagnose and monitor certain medical conditions, such as carbohydrate-deficient glycoprotein syndrome, a rare inherited disorder affecting the structure of glycoproteins.

In the context of medicine and pharmacology, "kinetics" refers to the study of how a drug moves throughout the body, including its absorption, distribution, metabolism, and excretion (often abbreviated as ADME). This field is called "pharmacokinetics."

1. Absorption: This is the process of a drug moving from its site of administration into the bloodstream. Factors such as the route of administration (e.g., oral, intravenous, etc.), formulation, and individual physiological differences can affect absorption.

2. Distribution: Once a drug is in the bloodstream, it gets distributed throughout the body to various tissues and organs. This process is influenced by factors like blood flow, protein binding, and lipid solubility of the drug.

3. Metabolism: Drugs are often chemically modified in the body, typically in the liver, through processes known as metabolism. These changes can lead to the formation of active or inactive metabolites, which may then be further distributed, excreted, or undergo additional metabolic transformations.

4. Excretion: This is the process by which drugs and their metabolites are eliminated from the body, primarily through the kidneys (urine) and the liver (bile).

Understanding the kinetics of a drug is crucial for determining its optimal dosing regimen, potential interactions with other medications or foods, and any necessary adjustments for special populations like pediatric or geriatric patients, or those with impaired renal or hepatic function.

Immunoelectrophoresis (IEP) is a laboratory technique used in the field of clinical pathology and immunology. It is a method for separating and identifying proteins, particularly immunoglobulins or antibodies, in a sample. This technique combines the principles of electrophoresis, which separates proteins based on their electric charge and size, with immunological reactions, which detect specific proteins using antigen-antibody interactions.

In IEP, a protein sample is first separated by electrophoresis in an agarose or agar gel matrix on a glass slide or in a test tube. After separation, an antibody specific to the protein of interest is layered on top of the gel and allowed to diffuse towards the separated proteins. This creates a reaction between the antigen (protein) and the antibody, forming a visible precipitate at the point where they meet. The precipitate line's position and intensity can then be analyzed to identify and quantify the protein of interest.

Immunoelectrophoresis is particularly useful in diagnosing various medical conditions, such as immunodeficiency disorders, monoclonal gammopathies (like multiple myeloma), and other plasma cell dyscrasias. It can help detect abnormal protein patterns, quantify specific immunoglobulins, and identify the presence of M-proteins or Bence Jones proteins, which are indicative of monoclonal gammopathies.

Glucans are polysaccharides (complex carbohydrates) that are made up of long chains of glucose molecules. They can be found in the cell walls of certain plants, fungi, and bacteria. In medicine, beta-glucans derived from yeast or mushrooms have been studied for their potential immune-enhancing effects. However, more research is needed to fully understand their role and effectiveness in human health.

Solubility is a fundamental concept in pharmaceutical sciences and medicine, which refers to the maximum amount of a substance (solute) that can be dissolved in a given quantity of solvent (usually water) at a specific temperature and pressure. Solubility is typically expressed as mass of solute per volume or mass of solvent (e.g., grams per liter, milligrams per milliliter). The process of dissolving a solute in a solvent results in a homogeneous solution where the solute particles are dispersed uniformly throughout the solvent.

Understanding the solubility of drugs is crucial for their formulation, administration, and therapeutic effectiveness. Drugs with low solubility may not dissolve sufficiently to produce the desired pharmacological effect, while those with high solubility might lead to rapid absorption and short duration of action. Therefore, optimizing drug solubility through various techniques like particle size reduction, salt formation, or solubilization is an essential aspect of drug development and delivery.

Temperature, in a medical context, is a measure of the degree of hotness or coldness of a body or environment. It is usually measured using a thermometer and reported in degrees Celsius (°C), degrees Fahrenheit (°F), or kelvin (K). In the human body, normal core temperature ranges from about 36.5-37.5°C (97.7-99.5°F) when measured rectally, and can vary slightly depending on factors such as time of day, physical activity, and menstrual cycle. Elevated body temperature is a common sign of infection or inflammation, while abnormally low body temperature can indicate hypothermia or other medical conditions.

High-performance liquid chromatography (HPLC) is a type of chromatography that separates and analyzes compounds based on their interactions with a stationary phase and a mobile phase under high pressure. The mobile phase, which can be a gas or liquid, carries the sample mixture through a column containing the stationary phase.

In HPLC, the mobile phase is a liquid, and it is pumped through the column at high pressures (up to several hundred atmospheres) to achieve faster separation times and better resolution than other types of liquid chromatography. The stationary phase can be a solid or a liquid supported on a solid, and it interacts differently with each component in the sample mixture, causing them to separate as they travel through the column.

HPLC is widely used in analytical chemistry, pharmaceuticals, biotechnology, and other fields to separate, identify, and quantify compounds present in complex mixtures. It can be used to analyze a wide range of substances, including drugs, hormones, vitamins, pigments, flavors, and pollutants. HPLC is also used in the preparation of pure samples for further study or use.

Dextrins are a group of carbohydrates that are produced by the hydrolysis of starches. They are made up of shorter chains of glucose molecules than the original starch, and their molecular weight and physical properties can vary depending on the degree of hydrolysis. Dextrins are often used in food products as thickeners, stabilizers, and texturizers, and they also have applications in industry as adhesives and binders. In a medical context, dextrins may be used as a source of calories for patients who have difficulty digesting other types of carbohydrates.

Electrophoresis is a laboratory technique used in the field of molecular biology and chemistry to separate charged particles, such as DNA, RNA, or proteins, based on their size and charge. This technique uses an electric field to drive the movement of these charged particles through a medium, such as gel or liquid.

In electrophoresis, the sample containing the particles to be separated is placed in a matrix, such as a gel or a capillary tube, and an electric current is applied. The particles in the sample have a net charge, either positive or negative, which causes them to move through the matrix towards the oppositely charged electrode.

The rate at which the particles move through the matrix depends on their size and charge. Larger particles move more slowly than smaller ones, and particles with a higher charge-to-mass ratio move faster than those with a lower charge-to-mass ratio. By comparing the distance that each particle travels in the matrix, researchers can identify and quantify the different components of a mixture.

Electrophoresis has many applications in molecular biology and medicine, including DNA sequencing, genetic fingerprinting, protein analysis, and diagnosis of genetic disorders.

'Clostridium cellulolyticum' is a species of gram-positive, rod-shaped, anaerobic bacteria found in soil and aquatic environments. It is known for its ability to break down complex carbohydrates such as cellulose and hemicellulose into simple sugars through the process of fermentation. This makes it a potential candidate for biofuel production from plant biomass.

The bacterium produces a range of enzymes that can degrade these polysaccharides, including cellulases and xylanases. These enzymes work together in a complex system to break down the cellulose and hemicellulose into monosaccharides, which can then be fermented by the bacterium to produce various end products such as acetate, ethanol, hydrogen, and carbon dioxide.

'Clostridium cellulolyticum' is also known to produce a number of other enzymes and metabolites that have potential applications in industry, including amylases, proteases, and lipases. However, further research is needed to fully understand the biology and potential uses of this organism.

Ammonium sulfate is a chemical compound with the formula (NH4)2SO4. It is a white crystalline solid that is highly soluble in water and is commonly used in fertilizers due to its high nitrogen content. In a medical context, it can be used as a laxative or for lowering the pH of the gastrointestinal tract in certain medical conditions. It may also be used in the treatment of metabolic alkalosis, a condition characterized by an excessively high pH in the blood. However, its use in medical treatments is less common than its use in agricultural and industrial applications.

Fermentation is a metabolic process in which an organism converts carbohydrates into alcohol or organic acids using enzymes. In the absence of oxygen, certain bacteria, yeasts, and fungi convert sugars into carbon dioxide, hydrogen, and various end products, such as alcohol, lactic acid, or acetic acid. This process is commonly used in food production, such as in making bread, wine, and beer, as well as in industrial applications for the production of biofuels and chemicals.

Isoelectric focusing (IEF) is a technique used in electrophoresis, which is a method for separating proteins or other molecules based on their electrical charges. In IEF, a mixture of ampholytes (molecules that can carry both positive and negative charges) is used to create a pH gradient within a gel matrix. When an electric field is applied, the proteins or molecules migrate through the gel until they reach the point in the gradient where their net charge is zero, known as their isoelectric point (pI). At this point, they focus into a sharp band and stop moving, resulting in a highly resolved separation of the different components based on their pI. This technique is widely used in protein research for applications such as protein identification, characterization, and purification.

Amino acids are organic compounds that serve as the building blocks of proteins. They consist of a central carbon atom, also known as the alpha carbon, which is bonded to an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom (H), and a variable side chain (R group). The R group can be composed of various combinations of atoms such as hydrogen, oxygen, sulfur, nitrogen, and carbon, which determine the unique properties of each amino acid.

There are 20 standard amino acids that are encoded by the genetic code and incorporated into proteins during translation. These include:

1. Alanine (Ala)
2. Arginine (Arg)
3. Asparagine (Asn)
4. Aspartic acid (Asp)
5. Cysteine (Cys)
6. Glutamine (Gln)
7. Glutamic acid (Glu)
8. Glycine (Gly)
9. Histidine (His)
10. Isoleucine (Ile)
11. Leucine (Leu)
12. Lysine (Lys)
13. Methionine (Met)
14. Phenylalanine (Phe)
15. Proline (Pro)
16. Serine (Ser)
17. Threonine (Thr)
18. Tryptophan (Trp)
19. Tyrosine (Tyr)
20. Valine (Val)

Additionally, there are several non-standard or modified amino acids that can be incorporated into proteins through post-translational modifications, such as hydroxylation, methylation, and phosphorylation. These modifications expand the functional diversity of proteins and play crucial roles in various cellular processes.

Amino acids are essential for numerous biological functions, including protein synthesis, enzyme catalysis, neurotransmitter production, energy metabolism, and immune response regulation. Some amino acids can be synthesized by the human body (non-essential), while others must be obtained through dietary sources (essential).

'Clostridium cellulovorans' is a species of gram-positive, rod-shaped, anaerobic bacteria that is commonly found in soil and aquatic environments. It is known for its ability to break down complex carbohydrates, such as cellulose and xylan, into simpler sugars, which it then ferments to produce various end products, including acetate, ethanol, hydrogen, and carbon dioxide.

The bacterium is of interest in the field of bioenergy, as its ability to efficiently convert plant biomass into useful chemicals has potential applications in the production of biofuels and other bioproducts. Additionally, 'C. cellulovorans' has been studied for its potential use in bioremediation, as it is capable of degrading a variety of pollutants, including polycyclic aromatic hydrocarbons (PAHs) and pesticides.

It is important to note that while 'C. cellulovorans' is generally considered to be a non-pathogenic bacterium, it can cause infections in individuals with compromised immune systems or underlying medical conditions. As with any potential pathogen, appropriate precautions should be taken when handling this organism in the laboratory setting.

In the context of medical terminology, tablets refer to pharmaceutical dosage forms that contain various active ingredients. They are often manufactured in a solid, compressed form and can be administered orally. Tablets may come in different shapes, sizes, colors, and flavors, depending on their intended use and the manufacturer's specifications.

Some tablets are designed to disintegrate or dissolve quickly in the mouth, making them easier to swallow, while others are formulated to release their active ingredients slowly over time, allowing for extended drug delivery. These types of tablets are known as sustained-release or controlled-release tablets.

Tablets may contain a single active ingredient or a combination of several ingredients, depending on the intended therapeutic effect. They are typically manufactured using a variety of excipients, such as binders, fillers, and disintegrants, which help to hold the tablet together and ensure that it breaks down properly when ingested.

Overall, tablets are a convenient and widely used dosage form for administering medications, offering patients an easy-to-use and often palatable option for receiving their prescribed treatments.

Immunodiffusion is a laboratory technique used in immunology to detect and measure the presence of specific antibodies or antigens in a sample. It is based on the principle of diffusion, where molecules move from an area of high concentration to an area of low concentration until they reach equilibrium. In this technique, a sample containing an unknown quantity of antigen or antibody is placed in a gel or agar medium that contains a known quantity of antibody or antigen, respectively.

The two substances then diffuse towards each other and form a visible precipitate at the point where they meet and reach equivalence, which indicates the presence and quantity of the specific antigen or antibody in the sample. There are several types of immunodiffusion techniques, including radial immunodiffusion (RID) and double immunodiffusion (Ouchterlony technique). These techniques are widely used in diagnostic laboratories to identify and measure various antigens and antibodies, such as those found in infectious diseases, autoimmune disorders, and allergic reactions.

Peptococcaceae is a family of obligately anaerobic, non-spore forming, gram-positive cocci that are found as normal flora in the human gastrointestinal tract. These bacteria are commonly isolated from feces and are known to be associated with various human infections, particularly intra-abdominal abscesses, bacteremia, and brain abscesses. The genus Peptococcus includes several species, such as Peptococcus niger and Peptococcus saccharolyticus, which are known to be associated with human infections. However, it is important to note that the taxonomy of this group of bacteria has undergone significant revisions in recent years, and some species previously classified as Peptococcaceae have been reassigned to other families.

Hydrolysis is a chemical process, not a medical one. However, it is relevant to medicine and biology.

Hydrolysis is the breakdown of a chemical compound due to its reaction with water, often resulting in the formation of two or more simpler compounds. In the context of physiology and medicine, hydrolysis is a crucial process in various biological reactions, such as the digestion of food molecules like proteins, carbohydrates, and fats. Enzymes called hydrolases catalyze these hydrolysis reactions to speed up the breakdown process in the body.

Psyllium is a type of fiber derived from the seeds of the Plantago ovata plant. It's often used as a bulk-forming laxative to help promote regularity and relieve constipation. When psyllium comes into contact with water, it swells and forms a gel-like substance that helps move waste through the digestive tract. In addition to its laxative effects, psyllium has also been shown to help lower cholesterol levels and control blood sugar levels in people with diabetes. It's available in various forms such as powder, capsules, and wafers, and can be found in many over-the-counter supplements and medications.

I'm sorry for any confusion, but "Wood" is not a medical term. It is a common name for various hard, fibrous tissues that make up the trunks and branches of trees and shrubs, as well as a term used for a wide range of items made from these materials. If you have any medical concerns or questions, I would be happy to try and help answer those for you.

Enzyme stability refers to the ability of an enzyme to maintain its structure and function under various environmental conditions, such as temperature, pH, and the presence of denaturants or inhibitors. A stable enzyme retains its activity and conformation over time and across a range of conditions, making it more suitable for industrial and therapeutic applications.

Enzymes can be stabilized through various methods, including chemical modification, immobilization, and protein engineering. Understanding the factors that affect enzyme stability is crucial for optimizing their use in biotechnology, medicine, and research.

I believe there may be some confusion in your question. "Rabbits" is a common name used to refer to the Lagomorpha species, particularly members of the family Leporidae. They are small mammals known for their long ears, strong legs, and quick reproduction.

However, if you're referring to "rabbits" in a medical context, there is a term called "rabbit syndrome," which is a rare movement disorder characterized by repetitive, involuntary movements of the fingers, resembling those of a rabbit chewing. It is also known as "finger-chewing chorea." This condition is usually associated with certain medications, particularly antipsychotics, and typically resolves when the medication is stopped or adjusted.

Glucan 1,4-beta-Glucosidase is an enzyme that breaks down certain types of complex carbohydrates known as beta-glucans. Specifically, this enzyme hydrolyzes the beta-1,4 glycosidic bonds in glucans, releasing individual glucose molecules. It is found in various organisms, including bacteria, fungi, and plants. In humans, it plays a role in the digestion of certain dietary fibers and may have potential applications in medical and industrial settings, such as in the treatment of bacterial infections or the production of biofuels.

Xylosidases are a group of enzymes that catalyze the hydrolysis of xylosides, which are glycosides with a xylose sugar. Specifically, they cleave the terminal β-1,4-linked D-xylopyranoside residues from various substrates such as xylooligosaccharides and xylan. These enzymes play an important role in the breakdown and metabolism of plant-derived polysaccharides, particularly hemicelluloses, which are a major component of plant biomass. Xylosidases have potential applications in various industrial processes, including biofuel production and animal feed manufacturing.

Centrifugation, Density Gradient is a medical laboratory technique used to separate and purify different components of a mixture based on their size, density, and shape. This method involves the use of a centrifuge and a density gradient medium, such as sucrose or cesium chloride, to create a stable density gradient within a column or tube.

The sample is carefully layered onto the top of the gradient and then subjected to high-speed centrifugation. During centrifugation, the particles in the sample move through the gradient based on their size, density, and shape, with heavier particles migrating faster and further than lighter ones. This results in the separation of different components of the mixture into distinct bands or zones within the gradient.

This technique is commonly used to purify and concentrate various types of biological materials, such as viruses, organelles, ribosomes, and subcellular fractions, from complex mixtures. It allows for the isolation of pure and intact particles, which can then be collected and analyzed for further study or use in downstream applications.

In summary, Centrifugation, Density Gradient is a medical laboratory technique used to separate and purify different components of a mixture based on their size, density, and shape using a centrifuge and a density gradient medium.

Pharmaceutical chemistry is a branch of chemistry that deals with the design, synthesis, and development of chemical entities used as medications. It involves the study of drugs' physical, chemical, and biological properties, as well as their interactions with living organisms. This field also encompasses understanding the absorption, distribution, metabolism, and excretion (ADME) of drugs in the body, which are critical factors in drug design and development. Pharmaceutical chemists often work closely with biologists, medical professionals, and engineers to develop new medications and improve existing ones.

I apologize for any confusion, but "Polyporaceae" is not a medical term. It is a taxonomic category in mycology, which is the branch of biology that deals with the study of fungi.

Polyporaceae is a family of fungi that includes various types of polypores, which are characterized by having pores on the underside of their fruiting bodies (the above-ground reproductive structures) instead of gills. These fungi are mostly saprotrophic, meaning they decompose dead or decaying organic matter to obtain nutrients. Some species can also be parasitic and cause diseases in living trees.

Examples of genera within Polyporaceae include Trametes, Ganoderma, Fomes, and Irpex, among others. If you have any questions related to medical terminology or concepts, please feel free to ask!

"Cattle" is a term used in the agricultural and veterinary fields to refer to domesticated animals of the genus *Bos*, primarily *Bos taurus* (European cattle) and *Bos indicus* (Zebu). These animals are often raised for meat, milk, leather, and labor. They are also known as bovines or cows (for females), bulls (intact males), and steers/bullocks (castrated males). However, in a strict medical definition, "cattle" does not apply to humans or other animals.

'Escherichia coli' (E. coli) is a type of gram-negative, facultatively anaerobic, rod-shaped bacterium that commonly inhabits the intestinal tract of humans and warm-blooded animals. It is a member of the family Enterobacteriaceae and one of the most well-studied prokaryotic model organisms in molecular biology.

While most E. coli strains are harmless and even beneficial to their hosts, some serotypes can cause various forms of gastrointestinal and extraintestinal illnesses in humans and animals. These pathogenic strains possess virulence factors that enable them to colonize and damage host tissues, leading to diseases such as diarrhea, urinary tract infections, pneumonia, and sepsis.

E. coli is a versatile organism with remarkable genetic diversity, which allows it to adapt to various environmental niches. It can be found in water, soil, food, and various man-made environments, making it an essential indicator of fecal contamination and a common cause of foodborne illnesses. The study of E. coli has contributed significantly to our understanding of fundamental biological processes, including DNA replication, gene regulation, and protein synthesis.

"Gossypium" is the scientific name for the cotton plant. It belongs to the Malvaceae family and is native to tropical and subtropical regions around the world. The cotton plant produces soft, fluffy fibers that are used to make a wide variety of textiles, including clothing, bedding, and other household items.

The medical community may use the term "Gossypium" in certain contexts, such as when discussing allergic reactions or sensitivities to cotton products. However, it is more commonly used in botany and agriculture than in medical terminology.

Xylan Endo-1,3-beta-Xylosidase is an enzyme that breaks down xylan, which is a major component of hemicellulose in plant cell walls. This enzyme specifically catalyzes the hydrolysis of 1,3-beta-D-xylosidic linkages in xylans, resulting in the release of xylose units from the xylan backbone. It is involved in the process of breaking down plant material for various industrial applications and in the natural decomposition of plants by microorganisms.

Ruminococcus is a genus of obligate anaerobic, gram-positive bacteria that are commonly found in the gastrointestinal tracts of humans and other animals. These bacteria play a crucial role in breaking down complex carbohydrates and fibers in the gut through fermentation, producing short-chain fatty acids (SCFAs) as byproducts. Ruminococcus species are particularly abundant in the rumen of ruminants such as cows and sheep, where they help to digest plant material. In humans, Ruminococcus species have been associated with various aspects of health and disease, including gut inflammation, colon cancer, and metabolic disorders. However, more research is needed to fully understand the complex relationship between these bacteria and human health.

In a medical context, "hot temperature" is not a standard medical term with a specific definition. However, it is often used in relation to fever, which is a common symptom of illness. A fever is typically defined as a body temperature that is higher than normal, usually above 38°C (100.4°F) for adults and above 37.5-38°C (99.5-101.3°F) for children, depending on the source.

Therefore, when a medical professional talks about "hot temperature," they may be referring to a body temperature that is higher than normal due to fever or other causes. It's important to note that a high environmental temperature can also contribute to an elevated body temperature, so it's essential to consider both the body temperature and the environmental temperature when assessing a patient's condition.

Isoenzymes, also known as isoforms, are multiple forms of an enzyme that catalyze the same chemical reaction but differ in their amino acid sequence, structure, and/or kinetic properties. They are encoded by different genes or alternative splicing of the same gene. Isoenzymes can be found in various tissues and organs, and they play a crucial role in biological processes such as metabolism, detoxification, and cell signaling. Measurement of isoenzyme levels in body fluids (such as blood) can provide valuable diagnostic information for certain medical conditions, including tissue damage, inflammation, and various diseases.

Fibrobacter is a genus of anaerobic, gram-negative bacteria that primarily resides in the gastrointestinal tracts of ruminants and other herbivorous animals. These bacteria are specialized in breaking down complex plant fibers, such as cellulose and xylan, into simpler sugars through fermentation. This process plays a crucial role in the digestion and nutrient acquisition from plant-based diets in these animals.

In human medicine, Fibrobacter species have been found in the oral cavity and gastrointestinal tract, but their significance in human health and disease is not well understood. Some studies suggest that an increased abundance of Fibrobacter may be associated with certain gut disorders like irritable bowel syndrome or inflammatory bowel disease; however, more research is needed to establish a clear relationship and understand the underlying mechanisms.

A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.

In the context of medical terminology, "powders" do not have a specific technical definition. However, in a general sense, powders refer to dry, finely ground or pulverized solid substances that can be dispersed in air or liquid mediums. In medicine, powders may include various forms of medications, such as crushed tablets or capsules, which are intended to be taken orally, mixed with liquids, or applied topically. Additionally, certain medical treatments and therapies may involve the use of medicated powders for various purposes, such as drying agents, abrasives, or delivery systems for active ingredients.

Mitosporic fungi, also known as asexual fungi or anamorphic fungi, are a group of fungi that produce mitospores (also called conidia) during their asexual reproduction. Mitospores are produced from the tip of specialized hyphae called conidiophores and are used for dispersal and survival of the fungi in various environments. These fungi do not have a sexual reproductive stage or it has not been observed, making their taxonomic classification challenging. They are commonly found in soil, decaying organic matter, and water, and some of them can cause diseases in humans, animals, and plants. Examples of mitosporic fungi include Aspergillus, Penicillium, and Fusarium species.

Arabidopsis proteins refer to the proteins that are encoded by the genes in the Arabidopsis thaliana plant, which is a model organism commonly used in plant biology research. This small flowering plant has a compact genome and a short life cycle, making it an ideal subject for studying various biological processes in plants.

Arabidopsis proteins play crucial roles in many cellular functions, such as metabolism, signaling, regulation of gene expression, response to environmental stresses, and developmental processes. Research on Arabidopsis proteins has contributed significantly to our understanding of plant biology and has provided valuable insights into the molecular mechanisms underlying various agronomic traits.

Some examples of Arabidopsis proteins include transcription factors, kinases, phosphatases, receptors, enzymes, and structural proteins. These proteins can be studied using a variety of techniques, such as biochemical assays, protein-protein interaction studies, and genetic approaches, to understand their functions and regulatory mechanisms in plants.

Endo-1,4-beta Xylanases are a type of enzyme that catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans, which are complex polysaccharides made up of beta-1,4-linked xylose residues. Xylan is a major hemicellulose component found in the cell walls of plants, and endo-1,4-beta Xylanases play an important role in the breakdown and digestion of plant material by various organisms, including bacteria, fungi, and animals. These enzymes are widely used in industrial applications, such as biofuel production, food processing, and pulp and paper manufacturing, to break down xylans and improve the efficiency of various processes.

A plant stem is not typically defined in a medical context, but rather in the field of botany. However, I can certainly provide a general biological definition for you.

In plants, stems are organs that serve primarily as support structures, holding leaves, flowers, and fruits aloft where they can receive sunlight and exchange gases. They also act as conduits, transporting water, nutrients, and sugars made during photosynthesis between the roots and shoots of a plant.

The stem is usually composed of three main tissue systems: dermal, vascular, and ground. The dermal tissue system forms the outermost layer(s) of the stem, providing protection and sometimes participating in gas exchange. The vascular tissue system contains the xylem (which transports water and nutrients upward) and phloem (which transports sugars and other organic compounds downward). The ground tissue system, located between the dermal and vascular tissues, is responsible for food storage and support.

While not a direct medical definition, understanding the structure and function of plant stems can be relevant in fields such as nutrition, agriculture, and environmental science, which have implications for human health.

Artificial membranes are synthetic or man-made materials that possess properties similar to natural biological membranes, such as selective permeability and barrier functions. These membranes can be designed to control the movement of molecules, ions, or cells across them, making them useful in various medical and biotechnological applications.

Examples of artificial membranes include:

1. Dialysis membranes: Used in hemodialysis for patients with renal failure, these semi-permeable membranes filter waste products and excess fluids from the blood while retaining essential proteins and cells.
2. Hemofiltration membranes: Utilized in extracorporeal circuits to remove larger molecules, such as cytokines or inflammatory mediators, from the blood during critical illnesses or sepsis.
3. Drug delivery systems: Artificial membranes can be used to encapsulate drugs, allowing for controlled release and targeted drug delivery in specific tissues or cells.
4. Tissue engineering: Synthetic membranes serve as scaffolds for cell growth and tissue regeneration, guiding the formation of new functional tissues.
5. Biosensors: Artificial membranes can be integrated into biosensing devices to selectively detect and quantify biomolecules, such as proteins or nucleic acids, in diagnostic applications.
6. Microfluidics: Artificial membranes are used in microfluidic systems for lab-on-a-chip applications, enabling the manipulation and analysis of small volumes of fluids for various medical and biological purposes.

Scanning electron microscopy (SEM) is a type of electron microscopy that uses a focused beam of electrons to scan the surface of a sample and produce a high-resolution image. In SEM, a beam of electrons is scanned across the surface of a specimen, and secondary electrons are emitted from the sample due to interactions between the electrons and the atoms in the sample. These secondary electrons are then detected by a detector and used to create an image of the sample's surface topography. SEM can provide detailed images of the surface of a wide range of materials, including metals, polymers, ceramics, and biological samples. It is commonly used in materials science, biology, and electronics for the examination and analysis of surfaces at the micro- and nanoscale.

A hypocotyl is not a medical term per se, but it is a term used in the field of botany, which is a branch of biology that deals with the study of plants. Therefore, I'd be happy to provide you with a definition of hypocotyl in a botanical context:

The hypocotyl is the portion of the embryo or seedling of a plant that lies between the cotyledons (the embryonic leaves) and the radicle (the embryonic root). In other words, it is the stem-like structure that connects the shoot and the root systems in a developing plant.

When a seed germinates, the hypocotyl elongates and pushes the cotyledons upward through the soil, allowing the young plant to emerge into the light. The hypocotyl can vary in length depending on the species of plant, and its growth is influenced by various environmental factors such as light and temperature.

While the term "hypocotyl" may not be commonly used in medical contexts, understanding basic botanical concepts like this one can still be useful for healthcare professionals who work with patients who have plant-related allergies or other health issues related to plants.

Cotton fiber is not a medical term, but I can provide you with some information about cotton fibers in a general context.

Cotton fibers are soft, fluffy staple fibers that grow in a protective case called a boll around the seeds of cotton plants. Cotton fibers are primarily composed of cellulose, which is an organic compound and the main structural component of plant cells. These fibers are typically spun into yarn or thread and used to make a wide variety of textiles, including clothing and other soft goods.

Cotton fibers have some unique properties that make them suitable for medical applications as well:

1. Hypoallergenic: Cotton is generally considered hypoallergenic, meaning it is less likely to cause an allergic reaction compared to other materials. This makes cotton a popular choice for medical bandages, swabs, and other products that come into contact with the skin.
2. Absorbent: Cotton fibers are highly absorbent, which can be useful in medical settings for managing wounds, incontinence, or excessive sweating.
3. Breathable: Cotton is a breathable material, allowing air to pass through and helping to maintain a comfortable body temperature. This property makes cotton an excellent choice for medical garments, bedding, and other products that require good ventilation.
4. Comfortable: Cotton fibers are soft, lightweight, and gentle on the skin, making them a preferred material for medical textiles and clothing designed for people with sensitive skin or medical conditions like eczema or dermatitis.
5. Durable: Although cotton fibers can be delicate when wet, they are relatively strong and durable in dry conditions. This makes cotton an appropriate choice for reusable medical products like gowns, scrubs, and linens.

Biofuels are defined as fuels derived from organic materials such as plants, algae, and animal waste. These fuels can be produced through various processes, including fermentation, esterification, and transesterification. The most common types of biofuels include biodiesel, ethanol, and biogas.

Biodiesel is a type of fuel that is produced from vegetable oils or animal fats through a process called transesterification. It can be used in diesel engines with little or no modification and can significantly reduce greenhouse gas emissions compared to traditional fossil fuels.

Ethanol is a type of alcohol that is produced through the fermentation of sugars found in crops such as corn, sugarcane, and switchgrass. It is typically blended with gasoline to create a fuel known as E85, which contains 85% ethanol and 15% gasoline.

Biogas is a type of fuel that is produced through the anaerobic digestion of organic materials such as food waste, sewage sludge, and agricultural waste. It is composed primarily of methane and carbon dioxide and can be used to generate electricity or heat.

Overall, biofuels offer a renewable and more sustainable alternative to traditional fossil fuels, helping to reduce greenhouse gas emissions and decrease dependence on non-renewable resources.

'Arabidopsis' is a genus of small flowering plants that are part of the mustard family (Brassicaceae). The most commonly studied species within this genus is 'Arabidopsis thaliana', which is often used as a model organism in plant biology and genetics research. This plant is native to Eurasia and Africa, and it has a small genome that has been fully sequenced. It is known for its short life cycle, self-fertilization, and ease of growth, making it an ideal subject for studying various aspects of plant biology, including development, metabolism, and response to environmental stresses.

Adsorption is a process in which atoms, ions, or molecules from a gas, liquid, or dissolved solid accumulate on the surface of a material. This occurs because the particles in the adsorbate (the substance being adsorbed) have forces that attract them to the surface of the adsorbent (the material that the adsorbate is adhering to).

In medical terms, adsorption can refer to the use of materials with adsorptive properties to remove harmful substances from the body. For example, activated charcoal is sometimes used in the treatment of poisoning because it can adsorb a variety of toxic substances and prevent them from being absorbed into the bloodstream.

It's important to note that adsorption is different from absorption, which refers to the process by which a substance is taken up and distributed throughout a material or tissue.

Drug compounding is the process of combining, mixing, or altering ingredients to create a customized medication to meet the specific needs of an individual patient. This can be done for a variety of reasons, such as when a patient has an allergy to a certain ingredient in a mass-produced medication, or when a patient requires a different dosage or formulation than what is available commercially.

Compounding requires specialized training and equipment, and compounding pharmacists must follow strict guidelines to ensure the safety and efficacy of the medications they produce. Compounded medications are not approved by the U.S. Food and Drug Administration (FDA), but the FDA does regulate the ingredients used in compounding and has oversight over the practices of compounding pharmacies.

It's important to note that while compounding can provide benefits for some patients, it also carries risks, such as the potential for contamination or incorrect dosing. Patients should only receive compounded medications from reputable pharmacies that follow proper compounding standards and procedures.

The liver is a large, solid organ located in the upper right portion of the abdomen, beneath the diaphragm and above the stomach. It plays a vital role in several bodily functions, including:

1. Metabolism: The liver helps to metabolize carbohydrates, fats, and proteins from the food we eat into energy and nutrients that our bodies can use.
2. Detoxification: The liver detoxifies harmful substances in the body by breaking them down into less toxic forms or excreting them through bile.
3. Synthesis: The liver synthesizes important proteins, such as albumin and clotting factors, that are necessary for proper bodily function.
4. Storage: The liver stores glucose, vitamins, and minerals that can be released when the body needs them.
5. Bile production: The liver produces bile, a digestive juice that helps to break down fats in the small intestine.
6. Immune function: The liver plays a role in the immune system by filtering out bacteria and other harmful substances from the blood.

Overall, the liver is an essential organ that plays a critical role in maintaining overall health and well-being.

The rumen is the largest compartment of the stomach in ruminant animals, such as cows, goats, and sheep. It is a specialized fermentation chamber where microbes break down tough plant material into nutrients that the animal can absorb and use for energy and growth. The rumen contains billions of microorganisms, including bacteria, protozoa, and fungi, which help to break down cellulose and other complex carbohydrates in the plant material through fermentation.

The rumen is characterized by its large size, muscular walls, and the presence of a thick mat of partially digested food and microbes called the rumen mat or cud. The animal regurgitates the rumen contents periodically to chew it again, which helps to break down the plant material further and mix it with saliva, creating a more favorable environment for fermentation.

The rumen plays an essential role in the digestion and nutrition of ruminant animals, allowing them to thrive on a diet of low-quality plant material that would be difficult for other animals to digest.

Cytosol refers to the liquid portion of the cytoplasm found within a eukaryotic cell, excluding the organelles and structures suspended in it. It is the site of various metabolic activities and contains a variety of ions, small molecules, and enzymes. The cytosol is where many biochemical reactions take place, including glycolysis, protein synthesis, and the regulation of cellular pH. It is also where some organelles, such as ribosomes and vesicles, are located. In contrast to the cytosol, the term "cytoplasm" refers to the entire contents of a cell, including both the cytosol and the organelles suspended within it.

Uridine Diphosphate Glucose (UDP-glucose) is a nucleotide sugar that plays a crucial role in the synthesis and metabolism of carbohydrates in the body. It is formed from uridine triphosphate (UTP) and glucose-1-phosphate through the action of the enzyme UDP-glucose pyrophosphorylase.

UDP-glucose serves as a key intermediate in various biochemical pathways, including glycogen synthesis, where it donates glucose molecules to form glycogen, a large polymeric storage form of glucose found primarily in the liver and muscles. It is also involved in the biosynthesis of other carbohydrate-containing compounds such as proteoglycans and glycolipids.

Moreover, UDP-glucose is an essential substrate for the enzyme glucosyltransferase, which is responsible for adding glucose molecules to various acceptor molecules during the process of glycosylation. This post-translational modification is critical for the proper folding and functioning of many proteins.

Overall, UDP-glucose is a vital metabolic intermediate that plays a central role in carbohydrate metabolism and protein function.

Tetroses are a type of monosaccharides, which are simple sugars that cannot be broken down into simpler units by hydrolysis. Tetroses have four carbon atoms and are aldotetroses, meaning they contain an aldehyde functional group at the first carbon atom.

There are two naturally occurring tetroses: erythrose and threose. Erythrose has its hydroxyl groups on the second and fourth carbon atoms, while threose has its hydroxyl groups on the second and third carbon atoms. Tetroses can participate in various chemical reactions, including forming glycosidic bonds with other monosaccharides to create disaccharides or polysaccharides. However, tetroses are not as common as other monosaccharides, such as pentoses and hexoses.

... it can be adsorbed on chromatography columns containing DEAE-cellulose as the adsorbent; it has a pH optimum of 4.5, lower than ...
Alkaline phosphatase from E. coli can be purified using a DEAE-Cellulose matrix. A. phosphatase has a slight negative charge, ...
DEAE can be used as a precursor for DEAE-cellulose resin, which is commonly used in ion exchange chromatography. DEAE can also ... "Experiments and model for the surface tension of DEAE-PZ and DEAE-MEA aqueous solutions". The Journal of Chemical ... Diethylethanolamine (DEAE) is a chemical compound with the molecular formula C6H15NO. It is used as a precursor in the ...
DEAE is an anion exchange matrix that is produced from a positive side group of diethylaminoethyl bound to cellulose or ... Cellulose based medium can be used for the separation of large molecules as they contain large pores. Protein binding in this ... This is typically a resin or gel matrix consisting of agarose or cellulose beads with covalently bonded charged functional ... Mini columns were filled with DEAE-Sephadex A-50 and further eluted with tris- buffer sodium chloride at various concentrations ...
... which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. The enzyme-activity was inhibited ...
DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger. This exchange ... DEAE-C beads have diethylaminoethyl chains covalently bound to oxygen atoms on the D-glucose subunits of cellulose. Size- ... Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column ... DEAE-C is synthesized by an alkali-catalyzed reaction of cellulose (obtained from cotton fabric) with 2-chlorotriethylamine, ...
In bovine brain samples, 14-3-3 proteins were located in the 14th fraction eluting from a DEAE-cellulose column and in position ...
... on a DEAE-cellulose column. The eleven components can be divided into two different types based on their spectroscopic features ...
... cellulose MeSH D09.698.365.180.180 - cellobiose MeSH D09.698.365.180.200 - cellulose, oxidized MeSH D09.698.365.180.247 - deae- ... deae-dextran MeSH D09.698.365.272.300 - dextran sulfate MeSH D09.698.365.272.400 - iron-dextran complex MeSH D09.698.365.388 - ... cellulose MeSH D09.698.365.180.515 - lignin MeSH D09.698.365.180.663 - methylcellulose MeSH D09.698.365.180.663.329 - ...
DEAE)-cellulose column washes and a final phosphocellulose fractionation. The final extract contained 10% of the activity ...
... deae-cellulose MeSH E05.196.181.400.454 - chromatography, paper MeSH E05.196.181.400.454.655 - nucleotide mapping MeSH E05.196. ... cellulose acetate MeSH E05.196.401.220 - electrophoresis, gel, pulsed-field MeSH E05.196.401.250 - electrophoresis, gel, two- ...
These materials include DEAE-dextran, glass, polystyrene plastic, and acrylamide. In 1967, microcarrier development began when ... and cellulose. Not only are these materials easily obtained, but the natural materials provide attachment sites for cells and a ...
The licensed farmers may face DEA legal problems if they do not acquire DEA permits. As the DEA had not yet acted on their ... cellulose insulation, and wind generated power. Wounded Knee Massacre and burial site. The events at Wounded Knee represents a ... The law in the U.S. is enforced by the Drug Enforcement Administration (DEA). In August 2000 and July 2001, federal DEA agents ... The attempt was shut down by the DEA and several other agencies. The mixed grass prairie is home to a variety of animals. In ...
Next, hydroxyethyl cellulose (HEC) is added as a binder and the entire mixture is homogenized. The solvents are allowed to ... DEA), the capping agent and reducing agent, respectively. After about twenty hours, the silver nitrate is dissolved, forming ...
Its largest application is for the conversion of cellulose to cellulose acetate, which is a component of photographic film and ... DEA List II precursor, and restricted in many other countries. Acetic anhydride is an irritant and combustible liquid; it is ... when the demand for acetic anhydride increased due to the production of cellulose acetate. Due to its low cost, acetic ...
The viscosity of these products can be altered by adjusting their water content and concentration of cellulose or other gel- ... DEA) or animal-based ingredients. Some contain botanicals such as aloe vera or plant extracts. Warming lubricants contain ... The earliest water-based lubricants were cellulose ether or glycerin solutions. Products available today may have various ...
Artio (Dea Artio in the Gallo-Roman religion) was a Celtic bear goddess. Evidence of her worship has notably been found at Bern ... Its ability to digest cellulose is ascribed to the microbes in its gut. Bears must spend much of their time feeding in order to ...
CM (Carboxymethyl group, weak cation exchange) SP (sulphopropyl group, strong cation exchange) DEAE-Sepharose QFF Ion exchange ... "Synthesis of super hydrophilic cellulose-alpha zirconium phosphate ion exchange membrane via surface coating for the removal of ...
DEA agents wearing Level B hazmat suits. Fuel spills from the gas tank as pit crew members in their PPE Apollo spacesuit worn ... "Superhydrophobic modification of cellulose and cotton textiles: Methodologies and applications". Journal of Bioresources and ...
Triple DEA - Modes of operation - Implementation guidelines ISO 19045:2015 Ophthalmic optics - Contact lens care products - ... Characterization of cellulose nanocrystals ISO 19719:2010 Machine tools - Work holding chucks - Vocabulary ISO 19720 Building ...
Not only does hemp grow at a faster rate, but it also contains a high level of cellulose. This quick return means that paper ... DEA). Hemp production in Kentucky, formerly the United States' leading producer, resumed in 2014. Hemp production in North ... If the paper industry were to switch from wood to hemp for sourcing its cellulose fibers, the following benefits could be ... "Comparison of papermaking potential of wood and hemp cellulose pulps". Annals of Warsaw University of Life Sciences; SGGW ...
"DEA, Drug Scheduling". DEA. Archived from the original on 4 November 2008. Retrieved 31 October 2008. "Misuse of Drugs Act 1971 ... Inactive ingredients in alprazolam tablets and solutions include microcrystalline cellulose, corn starch, docusate sodium, ... "DEA Brief Benzodiazepines". Archived from the original on 12 March 2009. Retrieved 1 October 2011. Given the millions of ...
... that had been confiscated by the DEA in Louisville. The local soil and climate proved favorable for the test crops, which ... using the plant residues to manufacture cellulose instead of burning it; mechanizing the process of separating the fiber from ...
DEA) periodically promotes local take-back programs, as well as the National Take Back Initiative. Take-back programs in the US ... cellulose, protein, chitin and xylan for pharmaceutical packaging, to reduce the hostile effects to the environment.[citation ...

No FAQ available that match "deae cellulose"

No images available that match "deae cellulose"