Single cell studies of enzymatic hydrolysis of a tetramethylrhodamine labeled triglucoside in yeast. (1/1142)
Several hundred molecules of enzyme reaction products were detected in a single spheroplast from yeast cells incubated with a tetramethylrhodamine (TMR) labeled triglucoside, alpha-d-Glc(1-->2)alpha-d-Glc(1-->3)alpha-d-Glc-O(CH2)8CONHCH2- CH2NH- COTMR. Product detection was accomplished using capillary electrophoresis and laser induced fluorescence following the introduction of a single spheroplast into the separation capillary. The in vivo enzymatic hydrolysis of the TMR-trisaccharide involves at least two enzymes, limited by processing alpha-glucosidase I, producing TMR-disaccharide, TMR-monosaccharide, and the free TMR-linking arm. Hydrolysis was reduced by preincubation of the cells with the processing enzyme inhibitor castanospermine. Confocal laser scanning microscopy studies confirmed the uptake and internalization of fluorescent substrate. This single cell analysis methodology can be applied for the in vivo assay of any enzyme with a fluorescent substrate. (+info)Freeze-fracture studies of the developing cell surface. II. Particle-free membrane blisters on glutaraldehyde-fixed corneal fibroblasts are artefacts. (2/1142)
We describe, in sections and by freeze-fracture, four classes of intramembrane particle (IMP)-free membrane blebs or "blisters" associated with glutaraldehyde-fixed embryonic corneal fibroblasts: (a) Single blisters attached to the cell membrane; (b) free (detached) vesicles; (c) myelin figures; (d) multivesicular protrusions which resemble the "mounds" described by others on nerve growth cones. The IMP-free, membrane-bounded blisters contain no ground cytoplasm or organelles, in contrast to blebs on trypsin-isolated fibroblasts, which we show here do contain cytoplasm and IMP-rich membranes. That the IMP-free membrane blisters in embryonic corneas are artefacts of fixation is demonstrated by (a) their absence in replicas of fibroblasts frozen and fractured without prior aldehyde fixation and (b) their absence in sections of fibroblasts fixed in a combination of glutaraldehyde and osmium tetroxide. We suggest that the addition of osmium prevents postfixation movement of membrane lipids, especially the negatively charged "fluid" lipids which others have shown are capable of considerable mobility after aldehyde fixation alone. Recent literature has implicated membrane blistering in secretory processes and in growth of nerves, but before the functional significance of such IMP-free blisters is assessed, membrane mobility of the type shown here should be taken into consideration. (+info)Gene transfer to human pancreatic endocrine cells using viral vectors. (3/1142)
We have studied the factors that influence the efficiency of infection of human fetal and adult pancreatic endocrine cells with adenovirus, murine retrovirus, and lentivirus vectors all expressing the green fluorescent protein (Ad-GFP, MLV-GFP, and Lenti-GFP, respectively). Adenoviral but not retroviral vectors efficiently infected intact pancreatic islets and fetal islet-like cell clusters (ICCs) in suspension. When islets and ICCs were plated in monolayer culture, infection efficiency with all three viral vectors increased. Ad-GFP infected 90-95% of the cells, whereas infection with MLV-GFP and Lenti-GFP increased only slightly. Both exposure to hepatocyte growth factor/scatter factor (HGF/SF) and dispersion of the cells by removal from the culture dish and replating had substantial positive effects on the efficiency of infection with retroviral vectors. Studies of virus entry and cell replication revealed that cell dispersion and stimulation by HGF/SF may be acting through both mechanisms to increase the efficiency of retrovirus-mediated gene transfer. Although HGF/SF and cell dispersion increased the efficiency of infection with MLV-GFP, only rare cells with weak staining for insulin were infected, whereas approximately 25% of beta-cells were infected with Lenti-GFP. We conclude that adenovirus is the most potent vector for ex vivo overexpression of foreign genes in adult endocrine pancreatic cells and is the best vector for applications where high-level but transient expression is desired. Under the optimal conditions of cell dispersion plus HGF/SF, infection with MLV and lentiviral vectors is reasonably efficient and stable, but only lentiviral vectors efficiently infect pancreatic beta-cells. (+info)The ultrastructural organization of the contractile peripheral protein layer of the human erythroycte membrane. (4/1142)
Erythroycte ghosts fixed in glutaraldehyde were dehydrated in (a) alcohol or acetone, (b) propylene glycol followed by Epon and embedded in an epoxy resin. A water-soluble urea/glutaraldehyde mixture was also used. The aim was to study the structure of the peripheral protein layer, which contains spectrin and actin, in the absence of OsO4 induced denaturation changes. Ghost membranes prepared in this way had an asymmetrical quadrilaminar structure. A layer of amorphous peripheral protein +/- 18 nm in width covered the entire inner face of the membrane in the form of a coarse meshwork in both Wash I (haemoglobin-containing) and haemoglobin-free ghosts. Cations (Mg2+ or Ca2+, or Mg2+ plus ATP) had no apparent effect on its fine structure. In contrast, the corresponding layer in OsO4-fixed membranes was represented by scanty, fuzzy material attached to the unit membrane only at irregular intervals. The results demonstrate the superior ability of glutaraldehyde to preserve the peripheral protein layer in thin sections, and afford further support for the view that much of this protein normally exists in an unpolymerized state. (+info)Biliary excretion in primary rat hepatocytes cultured in a collagen-sandwich configuration. (5/1142)
The objective of the present investigation was to examine the functional reestablishment of polarity in freshly isolated hepatocytes cultured between 2 layers of gelled collagen (sandwich configuration). Immunoblot analysis demonstrated that the canalicular multispecific organic anion transport protein (multidrug resistance-associated protein, Mrp2) was partially maintained in day 5 hepatocytes cultured in a sandwich configuration. Fluorescein-labeled taurocholate and carboxydichlorofluorescein were excreted into and concentrated in the bile canalicular lumen of day 5 sandwich-cultured hepatocytes, resulting in formation of fluorescent networks in standard buffer (intact bile canaliculi). Confocal microscopy studies demonstrated that 1) carboxydichlorofluorescein that had concentrated in the canalicular lumen was released into the incubation buffer in the presence of Ca(2+)-free buffer (disrupted bile canaliculi), and 2) rhodamine-dextran, an extracellular space marker, was only able to diffuse into the canalicular lumen in the presence of Ca(2+)-free buffer. The cumulative uptake of [(3)H]taurocholate in day 5 sandwich-cultured hepatocytes was significantly higher in standard buffer compared with Ca(2+)-free buffer, due to accumulation of taurocholate in canalicular spaces. When [(3)H]taurocholate was preloaded in the day 5 sandwich-cultured hepatocytes, taurocholate efflux was greater in Ca(2+)-free compared with standard buffer. The biliary excretion index of taurocholate, equivalent to the percentage of retained taurocholate in the canalicular networks, increased from approximately 8% at day 0 to approximately 60% at day 5 in sandwich-cultured hepatocytes. In summary, hepatocytes cultured in a collagen-sandwich configuration for up to 5 days establish intact canalicular networks, maintain Mrp2, reestablish polarized excretion of organic anions and bile acids, and represent a useful in vitro model system to investigate the hepatobiliary disposition of substrates. (+info)Demonstration of a coupled metabolism-efflux process at the choroid plexus as a mechanism of brain protection toward xenobiotics. (6/1142)
Brain homeostasis depends on the composition of both brain interstitial fluid and CSF. Whereas the former is largely controlled by the blood-brain barrier, the latter is regulated by a highly specialized blood-CSF interface, the choroid plexus epithelium, which acts either by controlling the influx of blood-borne compounds, or by clearing deleterious molecules and metabolites from CSF. To investigate mechanisms of brain protection at the choroid plexus, the blood-CSF barrier was reconstituted in vitro by culturing epithelial cells isolated from newborn rat choroid plexuses of either the fourth or the lateral ventricle. The cells grown in primary culture on semipermeable membranes established a pure polarized monolayer displaying structural and functional barrier features, (tight junctions, high electric resistance, low permeability to paracellular markers) and maintaining tissue-specific markers (transthyretin) and specific transporters for micronutriments (amino acids, nucleosides). In particular, the high enzymatic drug metabolism capacity of choroid plexus was preserved in the in vitro blood-CSF interface. Using this model, we demonstrated that choroid plexuses can act as an absolute blood-CSF barrier toward 1-naphthol, a cytotoxic, lipophilic model compound, by a coupled metabolism-efflux mechanism. This compound was metabolized in situ via uridine diphosphate glururonosyltransferase-catalyzed conjugation, and the cellular efflux of the glucurono-conjugate was mediated by a transporter predominantly located at the basolateral, i.e., blood-facing membrane. The transport process was temperature-dependent, probenecid-sensitive, and recognized other glucuronides. Efflux of 1-naphthol metabolite was inhibited by intracellular glutathione S-conjugates. This metabolism-polarized efflux process adds a new facet to the understanding of the protective functions of choroid plexuses. (+info)Tissue factor activity is increased in human endothelial cells cultured under elevated static pressure. (7/1142)
We tested the hypothesis that elevated blood pressure, a known stimulus for vascular remodeling and an independent risk factor for the development of atherosclerotic disease, can modulate basal and cytokine-induced tissue factor (TF; CD 142) expression in cultured human endothelial cells (EC). Using a chromogenic enzymatic assay, we measured basal and tumor necrosis factor-alpha (TNF-alpha; 10 ng/ml, 5 h)-induced TF activities in human aortic EC (HAEC) and vena cava EC (HVCEC) cultured at atmospheric pressure and at 170 mmHg imposed pressure for up to 48 h. Basal TF activities were 22 +/- 10 U/mg protein for HAEC and 14 +/- 9 U/mg protein for HVCEC and were upregulated in both cell types >10-fold by TNF-alpha. Exposure to pressure for 5 h induced additional elevation of basal TF activity by 47 +/- 16% (P < 0.05, n = 6) for HAEC and 17 +/- 5% (P < 0.05, n = 3) for HVCEC. Pressurization also enhanced TF activity in TNF-alpha-treated cells from 240 +/- 28 to 319 +/- 32 U/mg protein in HAEC (P < 0.05, n = 4) and from 148 +/- 25 to 179 +/- 0.8 U/mg protein (P < 0.05, n = 3) in HVCEC. Cytokine stimulation caused an approximately 100-fold increase in steady-state TF mRNA levels in HAEC, whereas pressurization did not alter either TF mRNA or cell surface antigen expression, as determined by quantitative RT-PCR methodology and ELISA. Elevated pressure, however, modulated the EC plasma membrane organization and/or permeability as inferred from the increased cellular uptake of the fluorescent amphipathic dye merocyanine 540 (33 +/- 7%, P < 0.05). Our data suggest that elevated static pressure modulates the hemostatic potential of vascular cells by modifying the molecular organization of the plasma membrane. (+info)Culture and characterisation of epithelial cells from human pterygia. (8/1142)
BACKGROUND/AIMS: Pterygia are a common disorder of the ocular surface. The disease represents a chronic fibrovascular and degenerative process thought to originate at the conjunctival-corneal junction, where altered limbal stem cells are proposed to be the cell of origin. Extensive epidemiological evidence exists to implicate ultraviolet B irradiation in the pathogenesis of pterygia. To date no animal or in vitro culture model has been developed to test such an hypothesis. The aim of this study was to establish and characterise a pure population of epithelial cells derived from pterygium tissue. METHODS: Tissue specimens were obtained from patients undergoing pterygium excision. Explants were cultured in either serum free or serum supplemented medium. Primary and passaged cells were processed for light microscopy, analysed by flow cytometry, and characterised immunohistochemically using specific antibodies. RESULTS: In serum free culture, cuboidal cells with typical morphology of epithelial cells migrated from the pterygium explants from 3 days onwards and eventually formed a cohesive monolayer. Passaged cells consisted of 98.4% cytokeratin positive cells and demonstrated immunoreactivity for multiple cytokeratins, including AE1, AE3, AE5, but were negative for AE8. These cells also expressed an epithelial specific antigen, together with vimentin and mucin, as did epithelial cells in sections of pterygia. CONCLUSIONS: A relatively simple method of isolating pterygium epithelial cells has been established. Cultured pterygium epithelial cells are phenotypically and functionally similar to their in vivo counterparts with respect to keratin, vimentin, and mucin expression. In vitro assays using these cells may aid in elucidating the pathogenesis of pterygia. (+info)Cytological techniques refer to the methods and procedures used for the microscopic examination and study of cells, including preparation, staining, and analysis of cell samples, often obtained from bodily fluids or fine-needle aspirations, to diagnose diseases, particularly cancer.
Cytological techniques refer to the methods and procedures used to study individual cells, known as cytopathology. These techniques are used in the diagnosis and screening of various medical conditions, including cancer. The most common cytological technique is the Pap test, which involves collecting cells from the cervix and examining them for abnormalities. Other cytological techniques include fine-needle aspiration (FNA), which involves using a thin needle to withdraw cells from a tumor or lump, and body fluids analysis, which involves examining cells present in various bodily fluids such as urine, sputum, and pleural effusions. These techniques allow for the examination of cellular structure, morphology, and other characteristics to help diagnose and monitor diseases.
Microtechnique
Baker, J. R. (1933). Cytological Technique. London: Methuen And Co. Ltd. "celloidin - Wiktionary". en.wiktionary.org. Retrieved ... This technique is to place the material on the glass slide and remove it with the scalpel or to dissect needle, then add a drop ... This technique can be used for the preparation of tissue of animals and plants. For using under optical microscopy, the ... Celloidin technique is the procedure of embedding a specimen in celloidin. This method can be used for embedding large, hard ...
Marianne Kreidl
In 1953, publishing under the name "Marianne Kreidl", she co-authored an article on a new cytological technique with Rudolph ... Keller, Rudolph; Chiego, Bernard; Kreidl, Marianne (1953). "A New Cytological Technique". Cytologia. 18 (4): 322-329. doi: ...
Mallory's trichrome stain
Boon, Mathilde E.; Drijver, Johanna S. (1986). Routine Cytological Staining Techniques: Theoretical Background and Practice. ... The staining technique was first published in 1900 by Frank Burr Mallory, then a histologist at Harvard University Medical ... It uses the three stains: aniline blue, acid fuchsin, and orange G. As a result, this staining technique can reveal collagen, ...
John Baker (biologist)
1929 Cytological technique, 1933 Biology in everyday life, 1934 Chemical control of conception, with a chapter by H. M. ... 1958 Cytological technique; the principles underlying routine methods, 1960 Cell structure and its interpretation; essays ... 1945 Discovery of the uses of colouring agents in biological micro-technique, 1945 Path of science, by C. E. Kenneth Mees ... ...
Kathleen Margaret Cole
There she used cytological techniques to experiment on marine algae. This research established the focus of the remainder of ...
Karyotype
MJ, Kottler (1974). "From 48 to 46: cytological technique, preconception, and the counting of human chromosomes". Bull Hist Med ... Using this technique, it is possible to detect small alterations in the human genome, that cannot be detected through methods ... Considering the techniques of the time, these results were remarkable. Joe Hin Tjio working in Albert Levan's lab found the ... Cytogenetics employs several techniques to visualize different aspects of chromosomes: G-banding is obtained with Giemsa stain ...
Cytogenetics
Kottler, Malcolm Jay (1974). "From 48 to 46: cytological technique, preconception, and the counting of human chromosomes". ... Techniques were expanded to allow for culture of free amniocytes recovered from amniotic fluid, and elongation techniques for ... Techniques used include karyotyping, analysis of G-banded chromosomes, other cytogenetic banding techniques, as well as ... New techniques were needed to correct this error. Joe Hin Tjio working in Albert Levan's lab was responsible for finding the ...
Geographic tongue
Although rare, cytological techniques and biopsies can be done to aid in a clinical diagnosis. The cytological description can ...
Marion Elizabeth Stilwell Cave
In addition, her opinion and experiences on cytological squash techniques were requested during the formation of a Plant ... Cytological Abnormalities in Ovules After Pollen Irradiation". American Journal of Botany. 41 (6): 469-483. doi:10.1002/j.1537- ... During this time she worked in collaboration with South African phycologist Mary Pocock to pioneer techniques to count the ... Cytological Abnormalities in Ovules after Pollen Irradiation (1954) The Variable Chromosome Number in Astrephomene ...
Papanicolaou stain
... (also Papanicolaou's stain and Pap stain) is a multichromatic (multicolored) cytological staining technique ... Pap staining is used in the Pap smear (or Pap test) and is a reliable technique in cervical cancer screening in gynecology. The ... ISBN 978-1-4377-1781-5. Drury, R. A. B.; Wallington, E. A. (1980). Carleton's Histological Technique (5th ed.). Oxford ... 1982). The Theory and Practice of Histological Techniques (2nd ed.). Longman Group Limited. Dey, Pranab (2018). "Routine ...
Comparative genomic hybridization
... scale which may lead to the identification of candidate genes to be further explored by other cytological techniques. Through ... However, initial DNA yields of isolated BAC clones are low and DNA amplification techniques are necessary. These techniques ... The technique involves whole genome amplification from a single cell which is then used in the array CGH method. It may also be ... In both techniques, DNA from a reference (or control) sample and DNA from a test (or patient) sample are differentially ...
Lysosomal storage disease
In the late 1950s and early 1960s, de Duve and colleagues, using cell fractionation techniques, cytological studies, and ... The experimental technique of gene therapy may offer cures in the future. Ambroxol has recently been shown to increase activity ... Furthermore, chaperone therapy, a technique used to stabilize the defective enzymes produced by patients, is being examined for ...
Jérôme Lejeune
... that she worked without assistance from Lejeune to develop the necessary cytological and histological resources and techniques ... Technique pour la culture de fibroblastes in vitro" [Study of somatic chromosomes. Technique to culture fibroblasts in vitro]. ... one on the discovery of trisomy 21 and a second one about the cell culture techniques that Gautier had learned during a ... since she had been trained in both cell culture and tissue staining techniques in the United States. Turpin agreed to provide ...
Marcus Seymour Pembrey
... where pioneer work in cytological technique was going on, and then at Würzburg where he carried out research under Adolf Eugen ... where the pioneer work in cytological technique was still going on and at Wurzburg, Germany where he carried out research under ...
Journal of Cell Science
... or to the techniques that are used in cytological investigations". Subsequent editors include H. G. Callan and A. V. Grimstone ... Journal of Cell Sciences publishes original research articles and reports, techniques and resources, reviews, and primers ...
Amyloodinium ocellatum
Diagnosis of amyloodiniosis consists of observing typical clinical signs and using nonlethal techniques. A cytological approach ... these techniques are still limited to laboratory contexts. In parallel, immunological approaches such as ELISA (enzyme linked ...
Septic arthritis
Cytological analysis of the joint aspirate can point to septic arthritis. However, a negative culture and sensitivity test does ... Various clinical scenarios and technique-related factors may impact the validity of results of the culture and sensitivity. ... Additionally, results of cytological analysis, though important, should not be interpreted in isolation of the clinical ... In children, joint synovial fluid aspiration techniques aim at isolating the infectious organism by culture and sensitivity ...
Edward Laurens Mark
Bringing the cytological and histological approach with him to Harvard University in 1877, he was responsible for the ... introduction of advanced European microscopic techniques. He became assistant professor of zoology in 1883 and Hersey professor ... In his landmark cytological monograph published in 1881, Mark also conceived the parenthetical referencing for citation, also ...
Hybridoma technology
Immuno-cytological analysis of bone marrow, tissue aspirates, lymph nodes etc. with selected monoclonal antibodies help in the ... The first screening technique used is called ELISA. The hybridoma culture supernatant, secondary enzyme labeled conjugate, and ... Conventional cytological examination had not revealed any neoplastic cells. Three monoclonal antibodies (anti-CEA, Ca 1 and ... The use of immuno-cytochemical techniques increases diagnostic accuracy in these cases. Ghosh, Mason and Spriggs analysed 53 ...
Lumbar puncture
The procedure is typically performed under local anesthesia using a sterile technique. A hypodermic needle is used to access ... Fluid may be sent for biochemical, microbiological, and cytological analysis. Using ultrasound to landmark may increase success ...
Orthotospovirus
These inclusions can be seen in the light microscope with proper staining techniques. These inclusions can be diagnostic. ... Cytological studies of TSWV and INSV have shown that these viruses produce granular inclusions in the cytoplasm of infected ...
Chromatin bridge
Even though DAPI staining is quick, neither laboratory technique can guarantee the presence of the bridges under the ... When viewed under a fluorescence microscope and immunostained for cytological markers, these chromatin bridges appear to ... In addition, immunofluorescence may be used as a laboratory technique to tag cells with specific fluorophores using antibodies ... These phenomena are usually visualized using the laboratory techniques of staining and fluorescence microscopy. The faithful ...
DNA-DNA hybridization
This new DDH technique became the standard for bacterial taxonomy. Charles Sibley and Jon Ahlquist, pioneers of the technique, ... "Molecular Hybridization of Radioactive DNA to the DNA of Cytological Preparations." Kline Biology Tower, Yale University, 13 ... In 2004, a new DDH technique was described. This technique utilized microplates and colorimetrically labelled DNA to decrease ... This technique is not widely used on larger organisms where differences in species are easier to identify. In the late 1900s, ...
Smoked glass
Developmental and Cytological Methods. Cambridge [England] University Press. p. 15. ISBN 978-0-521-24915-7. "Development- ... Inspection of Gas-Turbine Components at D. Napier & Son, Ltd.: Recording Profiles on Smoked Glass: Equipment and Technique". ...
Lampbrush chromosome
Gall JG (1966) Techniques for the study of lampbrush chromosomes. In: Prescott DM (ed) Methods in cell physiology, vol II. ... lampbrush chromosomes are widely used for high-resolution mapping of DNA sequences and construction of detail cytological maps ... Gall JG (1966) Techniques for the study of lampbrush chromosomes. In: Prescott DM (ed) Methods in cell physiology, vol II. ...
Powdery mildew
April 2003). "Cytological Evidence of an Active Role of Silicon in Wheat Resistance to Powdery Mildew (Blumeria graminis f. sp ... 12th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-growing. pp. 87-92. ...
Clarence Erwin McClung
McClung took advantage of the great abundance of grasshoppers in Kansas "to make them pivotal for cytological study and to ... Development of the "McClung Model" research microscope and publication of the "Handbook of Microscopal Technique" demonstrated ... found a school of grasshopper cytologists." Later, the use of Brachystola magna ("Lubber" grasshopper) for cytological studies ...
Janet Elizabeth Macgregor
Macgregor took exfoliated cell smears using the Papanicolaou stain, interpreted them, and trained the team in the technique. In ... and that cervical cancer could largely be prevented by cytological detection and treatment of a pre-invasive stage. Having seen ...
Staining
StainsFile Reference for dyes and staining techniques. Vital Staining for Protozoa and Related Temporary Mounting Techniques ~ ... The papanicolaou stain is now used in place of cytological staining in all organ types due to its increase in morphological ... Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the ... Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently ...
Helena Slizynska
Hermann J. Muller, who was also staying at the Institute at the time, enrolled their cytological expertise in problems that ... developing a technique that made the results as reliable as humanly possible. Slizynska's most notable contribution was her ...
A comparison of rapid staining techniques for the cytological evaluation of canine intracranial neoplasms - Enlighten Theses
... and cytological techniques have been developed to aid intraoperative diagnosis from biopsy specimens. Stereotactic techniques ... Long, Sam Nicholas (2001) A comparison of rapid staining techniques for the cytological evaluation of canine intracranial ... A comparison of rapid staining techniques for the cytological evaluation of canine intracranial neoplasms ... veterinary literature evaluating the normal cytological appearance of the brain or comparing different cytological techniques ...
Microtechnique - Wikipedia
Baker, J. R. (1933). Cytological Technique. London: Methuen And Co. Ltd. "celloidin - Wiktionary". en.wiktionary.org. Retrieved ... This technique is to place the material on the glass slide and remove it with the scalpel or to dissect needle, then add a drop ... This technique can be used for the preparation of tissue of animals and plants. For using under optical microscopy, the ... Celloidin technique is the procedure of embedding a specimen in celloidin. This method can be used for embedding large, hard ...
topic:"Cytological Techniques" found 21 records - Provincial Hospitals Library Catalogue - Nova Scotia Health Authority
Clinical Laboratory Techniques. Cytological Techniques. Histological Techniques. Specialty. Clinical Laboratory Techniques. ... Cytological Techniques. Subjects (LCSH). Cytodiagnosis. Pathology, Cellular. Cytological Techniques. Abstract. In the newly ... Cytological Techniques. Notes. eReference--Publisher.. Contents. Acinic cell carcinoma, cytological findings -- Acute ... Cytological Techniques - methods. Histological Techniques - methods. Pancreas - pathology. Specialty. Gastroenterology. ...
NeoPath, Inc. NeoPath AutoPap 300 Automatic Pap Screener System
Assessment of the Roche Linear Array HPV Genotyping Test within the VALGENT framework
Differential and Cytological Staining Techniques (Procedure) : Microbiology Virtual Lab I : Biotechnology and Biomedical...
SciELO - Acta Botanica Brasilica, Volume: 17, Issue: 3, Published: 2003
Techniques to obtain cytological preparations with high frequency of mitotic metaphases in plants: Passiflora (Passifloraceae) ... The techniques were the usual for light microscopy. All the species presented the water reservoir specialized tissue originated ... Experiments were carried out aiming to optimize procedures for cytological preparations of root tips with high frequency of ...
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Tissue Embedding | Harvard Catalyst Profiles | Harvard Catalyst
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Inter Research » DAO » v42 » n3 » p199-206
The PCR assay developed here is more sensitive and less ambiguous than standard histological and cytological techniques. ... Sampling error and the subjectivity of cytological diagnoses are the likely sources of disagreement between diagnostic methods ... ABSTRACT: The development of diagnostic assays more sensitive and specific than traditional histological techniques is ...
Periodic Acid-Schiff Reaction | Profiles RNS
Clinical Diagnostics Market Research Reports & Consulting from MarketsandMarkets
This segment includes techniques like tissue biopsy, cytological smears, immunohistochemistry (IHC), and in situ hybridization ... These techniques provide detailed images that aid in the diagnosis and monitoring of various diseases and conditions. ... Imaging Techniques: Diagnostic imaging methods, such as X-rays, computed tomography (CT) scans, magnetic resonance imaging (MRI ... Multi-Modal Imaging Techniques: Advances in imaging technologies will continue to improve the resolution, sensitivity, and ...
Thiazin red as a neuropathological tool for the rapid diagnosis of Alzheimer's disease in tissue imprints | Acta...
Cytological demonstration of features of Alzheimers disease using brain smear technique. Diagn Cytopathol 10:320-325 ... Dada MA, Ansari NA (1997) Post-mortem cytology: a reappraisal of a little used technique. Cytopathology 8:417-420 ... comparison with other staining techniques. A study of 15 cases with graded intellectual status in ageing and Alzheimers ...
Flow Cytometry - MeSH - NCBI
... and Therapeutic Techniques and Equipment CategoryInvestigative TechniquesClinical Laboratory TechniquesCytological Techniques ... and Therapeutic Techniques and Equipment CategoryInvestigative TechniquesClinical Laboratory TechniquesCytological Techniques ... Diagnostic and Therapeutic Techniques and Equipment CategoryInvestigative TechniquesCytological TechniquesCell SeparationFlow ... Diagnostic and Therapeutic Techniques and Equipment CategoryInvestigative TechniquesCytological TechniquesCytophotometryFlow ...
Centrifugation techniques and reagent strips in the assessment of microhaematuria. | Journal of Clinical Pathology
Propolis: MedlinePlus Supplements
Comparison of nuclear texture analysis and image cytometric DNA analysis for the assessment of dysplasia in Barrett's...
A combination score based on both techniques performed better than either test in isolation. These data demonstrate that NT/ICM ... although promising new cytological techniques, that sample a larger field of the Barretts segment are being investigated (Lao- ... New single platform techniques to measure chromosomal instability, such as SnP and gene chip arrays (Paulson et al, 2009), are ... A combination score based on both techniques performed better than either test in isolation. These data demonstrate that NT/ICM ...
Laboratory techniques and procedures - Latest research and news | Nature
Betes Ib����ez, Mar��a Teresa. Curriculum. Universidad de Navarra
The most common technique was: a 22-G (65%) and cytological needle (75%) with suction -syringe (66%). No serious adverse events ... We will describe how the patient should be prepared, what accessories are needed and how the technique is performed. We will ... Primary tumor locations were lung70%, others19%, and unknown 11% AG samples were adequate for cytological diagnosis in 91%, and ... P , .05). There were very few clinically relevant complications related to EUS-FNA and EUS-EchoBrush techniques. CONCLUSIONS: ...
Combined Colleges: 2018-2019 Catalog - Course Descriptions for Veterinary Technology
Survey of microbiological techniques. Emphasis on laboratory procedures. The student will perform urinalysis, cytological, and ... Large Animal Assisting Techniques. This is a WECM Course Number. Prerequisite Required: VTHT 1301.. Course Description: Study ... Course Description: Survey of the profession of veterinary technology with emphasis on basic techniques, handling and care of ... Course Description: In-depth application of surgical, obstetrical, and anesthesia techniques including identification and use ...
Canine Microbial Overgrowth - WSAVA2006 - VIN
Several cytological techniques can be used: impression smear, « scotch test » using pieces of tape (clear cellophane) strip, ... Cytology is the most useful technique to demonstrate BOG. The tape strip technique is the most appropriate. A rapid staining is ... Cytological examination will show oval or elongated cells of 3 to 5µm in diameter, with a typical single polar budding. The ... Cytological examination may show yeasts and allow for a semi-quantification. The result is immediate using the immersion power ...
Histology, Otago Micro and Nanoscale Imaging | University of Otago
Histology services prepare samples for analysis using a variety of techniques for subsequent examination. ... Histology services prepare samples for analysis using a variety of techniques for subsequent examination. ... as well as staff with the necessary expertise to offer advice and guidance on histology and cytological techniques.. Read more ... Services and techniques. We are happy to discuss your potential project and provide advice and assistance on:. *Collection of ...
Bacterial Pyoderma - WSAVA2011 - VIN
The various techniques can be used for cytological examination, including impression smears, swabs, scrapings, acetate tape ... This technique is most often used for samples taken from draining tracts, ear canals, or interdigital webs. The cotton tip is ... This sampling technique is mainly used for fluid-containing lesions. The edge of the slide (papules, crusts, erosions, ...
MeSH Browser
exfoliative cytology
Mary Berbee - UBC Professor - Faculty Member - Researcher - Supervisor
Rather than adapt cytological techniques to describe the life cycle of S. tapetis, the most abundant species, I used population ... A different shade of hypha: cytological and molecular phylogenetic evidence for the independent rise of the hyphal habit in the ... I identified and described three new genera and six species using microscopy and molecular phylogenetic techniques. Two, which ... In Chapter 3, I discuss the significance of my research and possible future directions including cytological experiments on the ...
February | 2016 | New research findings from Princeton University
... the team used a laboratory technique referred to as bacterial cytological profiling to investigate the mode of action of TDA. ... The Northwestern team showed that when the kur gene was inactivated using a gene-editing technique called CRISPR-Cas9, the lack ... Using a special profiling technique, scientists at Princeton have determined the mechanism of action of a potent antibiotic, ... "The strength of this profiling technique is that it tells you how to repurpose molecules," Seyedsayamdost said. ...
Greg Smith, PhD Appointed Assistant Professor | Department of Genetics
CytologyInvestigative TechniquesBiochemicalSpecimensHistologicalDiagnosisExaminationStaining TechniquesSmearDiagnostic TechniquesSensitivityCervicalMethodsLesionsSmearsTissueIndicationsLaboratoryGeneticVeterinaryMolecularCombinationMortalityCellsObstetricalAnalysisEvaluationFoundTopicMethodGlass slideReviewSpecialManagement
Cytology6
- This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. (nshealth.ca)
- Dada MA, Ansari NA (1997) Post-mortem cytology: a reappraisal of a little used technique. (springer.com)
- Fine-needle aspiration biopsy (FNAB) is a useful technique in the evaluation of central lung tumors which is commonly encountered in clinical cytology practice. (sun.ac.za)
- Fully updated, the 4th edition of Cytology helps you apply the latest diagnostic techniques for the interpretation of a complete range of cytological specimens . (libribook.com)
- Background: Fineneedle aspiration cytology (FNAC) as a cheap diagnostic technique has helped reduce diagnostic turnaround time of pathological entities from different body sites. (bvsalud.org)
- Methodology: Cytology and corresponding histology reports for lesions from patients where both were available were compared for concordance between specific cytological diagnosis and final histological diagnosis. (bvsalud.org)
Investigative Techniques1
- Even in terms of diagnostics, it is clear that the Clinic for Haematology, Oncology and Gastroenterology boasts outstanding equipment: the full range of modern investigative techniques is on offer here, including cytological bone marrow diagnostics with special staining. (leading-medicine-guide.com)
Biochemical3
- Blood, urine, semen or tissue samples can be analysed using biochemical, microbiological and cytological methods. (nature.com)
- He built a solid foundation in inhalation toxicology - both in engineering and analytical techniques used to generate and characterize inhalation exposures as well as biochemical, cytological, histological, and physiological techniques to measure respiratory responses to airborne agents. (unc.edu)
- His research, which utilized Drosophila melanogaster as a model genetic system, incorporated molecular, genetic, cytological and biochemical techniques to characterize the role of the Orientation Disruptor (ORD) and dRING proteins in promoting normal sister-chromatid cohesion and chromosome condensation during meiosis. (hbsr.com)
Specimens5
- In humans, stereotactic needle biopsy has become a common method of providing a definitive diagnosis of brain tumours with safety and reliability, and cytological techniques have been developed to aid intraoperative diagnosis from biopsy specimens. (gla.ac.uk)
- Laboratory techniques and procedures are performed on patient specimens to detect biomarkers and diagnose diseases. (nature.com)
- Frozen sections of tissue and cytological specimens are also suitable for laser microdissection. (concordia.ca)
- Wish you could interpret cytological specimens in practice rather than paying a lab to do it for you? (fennovet.fi)
- It describes techniques for obtaining good quality cytological diagnostic specimens, and guides you through the interpretation of cytological findings. (fennovet.fi)
Histological5
- ABSTRACT: The development of diagnostic assays more sensitive and specific than traditional histological techniques is important for the management of bonamiasis in flat oysters Ostrea edulis . (int-res.com)
- The PCR assay developed here is more sensitive and less ambiguous than standard histological and cytological techniques. (int-res.com)
- The unit provides a well-equipped laboratory for performing histological analysis for diagnostic and research purposes, as well as staff with the necessary expertise to offer advice and guidance on histology and cytological techniques. (otago.ac.nz)
- As evidenced by the micrograph, combining phase contrast microscopy with classical histological staining techniques in pathological research often yields enhancement of cellular features. (fsu.edu)
- South Africa) with subsequent histological investigations and attempt to identify predictors of pathology relevant to the clinical management of women with a cytological diagnosis of AGC. (bvsalud.org)
Diagnosis4
- Stereotactic techniques have recently become available for use in dogs and cats, but little information has been published in the veterinary literature evaluating the normal cytological appearance of the brain or comparing different cytological techniques for intraoperative diagnosis. (gla.ac.uk)
- These techniques provide detailed images that aid in the diagnosis and monitoring of various diseases and conditions. (marketsandmarkets.com)
- The identification of salient cytological features and a high index of suspicion when considering the differential diagnosis of a central lung tumor is essential to accurate diagnosis. (sun.ac.za)
- Diagnosis of OC was performed in accordance with the International Classification System and cytological features. (bvsalud.org)
Examination4
- Cytological examination may show yeasts and allow for a semi-quantification. (vin.com)
- Histology services prepare samples for analysis using a variety of techniques for subsequent examination. (otago.ac.nz)
- The various techniques can be used for cytological examination, including impression smears, swabs, scrapings, acetate tape preparations and a fine needle aspiration. (vin.com)
- This technique is useful only for the examination of surface cells and often requires additional cytological analysis to confirm the results. (britannica.com)
Staining Techniques2
- Morphological Study of the Seminal Fluid -- 4.1 Preparation of Semen Smears -- 4.2 Staining Techniques -- 5. (nshealth.ca)
- He Y, Duyckaerts C, Delaere P, Piette F, Hauw JJ (1993) Alzheimer's lesions labelled by anti-ubiquitin antibodies: comparison with other staining techniques. (springer.com)
Smear5
- Samples were also taken from a variety of lesions at post mortem from 10 dogs and 1 cat to evaluate 3 preparation techniques (touch impression, medium pressure impression and smear preparation) and 4 stains (Diff-Quik, May-Grunwald- Giemsa, toluidine blue and Zynostain). (gla.ac.uk)
- The preparation techniques and stains examined provided good diagnostic accuracy, with smear preparation and Diff-Quick stains showing slightly greater accuracy than other techniques. (gla.ac.uk)
- McLean CA, Harney MC, Gonzales MF (1994) Cytological demonstration of features of Alzheimer's disease using brain smear technique. (springer.com)
- Several cytological techniques can be used: impression smear, « scotch test » using pieces of tape (clear cellophane) strip, scrape smear, swab smear. (vin.com)
- A cytological smear of human multipolar neurons stained with a mixture of eosin and hematoxylin is illustrated in the photomicrograph presented below. (fsu.edu)
Diagnostic Techniques1
- Molecular Diagnostics: Molecular diagnostic techniques involve analyzing DNA, RNA, and proteins to detect genetic variations, identify infectious agents, and assess disease markers. (marketsandmarkets.com)
Sensitivity2
- A study of the sensitivity of the different techniques for assessing microhaematuria seemed necessary. (bmj.com)
- In 2008, Mehrotra et al emphasized the value of the brush biopsy technique in evaluating oral lesions without the aid of computer-assisted technology, although they reported marginally lower sensitivity and specificity. (medscape.com)
Cervical2
- The VALGENT-3 framework comprised 1600 samples obtained from Slovenian women (1300 sequential cases from routine cervical cancer screening enriched with 300 cytological abnormal samples). (nih.gov)
- The main techniques used are cytological screening of cervical cells and visual inspection of the cervix. (who.int)
Methods2
- Sampling error and the subjectivity of cytological diagnoses are the likely sources of disagreement between diagnostic methods in oysters with very light infections. (int-res.com)
- Imaging Techniques: Diagnostic imaging methods, such as X-rays, computed tomography (CT) scans, magnetic resonance imaging (MRI), ultrasound, and nuclear medicine scans, are used to visualize internal structures and identify abnormalities in the body. (marketsandmarkets.com)
Lesions1
- This sampling technique is mainly used for fluid-containing lesions. (vin.com)
Smears1
- In order to evaluate the normal cytological appearance of the canine brain, smears were prepared from samples taken from a normal brain and stained with toluidine blue, following which the cytological appearance of different regions of the brain were described. (gla.ac.uk)
Tissue3
- This technique can be used for the preparation of tissue of animals and plants. (wikipedia.org)
- The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. (harvard.edu)
- The cytological sample was smeared onto a glass slide and either air-dried for May-Grünwald- stain, or ethanol-fixed for Papanicolaou stain and masses were surgically removed, the tumours were grossly examined and tissue samples were fixed in 10%-buffered-formalin and embedded in paraffin. (biomedcentral.com)
Indications1
- Covering indications for cytological investigation, collection techniques and the evaluation and interpretation of findings, this concise manual will be your go-to resource. (fennovet.fi)
Laboratory4
- It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. (nshealth.ca)
- Clinical diagnostics refers to the process of identifying, diagnosing, and monitoring diseases or medical conditions in patients through various laboratory tests, imaging techniques, and other diagnostic tools. (marketsandmarkets.com)
- Emphasis on practical reproductive techniques in laboratory settings. (dcccd.edu)
- In collaboration with researchers in the laboratory of Zemer Gitai , an associate professor of molecular biology at Princeton, the team used a laboratory technique referred to as bacterial cytological profiling to investigate the mode of action of TDA. (princeton.edu)
Genetic1
- Polymerase chain reaction (PCR), gene sequencing, microarrays, and other molecular techniques are used to identify specific genetic mutations, infectious agents, and biomarkers associated with diseases. (marketsandmarkets.com)
Veterinary1
- Survey of the profession of veterinary technology with emphasis on basic techniques, handling and care of animals, and ethical and professional requirements. (dcccd.edu)
Molecular1
- A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. (rush.edu)
Combination1
- A combination score based on both techniques performed better than either test in isolation. (nature.com)
Mortality1
- Housing and husbandry techniques were validated with 0% mortality and normal species-specific behaviors were observed. (researchsquare.com)
Cells4
- Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. (nih.gov)
- The Northwestern team showed that when the kur gene was inactivated using a gene-editing technique called CRISPR-Cas9, the lack of a functioning Kurly protein led to the mis-positioning of a second protein on the cell surface called Prickle2, which helps cells know which direction they face. (princeton.edu)
- The team used three dyes to evaluate 13 different features of the deceased cells, such as cell membrane thickness and nucleoid area, comprising TDA's cytological profile. (princeton.edu)
- A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. (uams.edu)
Obstetrical1
- In-depth application of surgical, obstetrical, and anesthesia techniques including identification and use of instruments and equipment. (dcccd.edu)
Analysis1
- In addition, this technique has been applied to other forms of analysis, including DNA image cytometry. (medscape.com)
Evaluation1
- Evaluation of housing, husbandry, clinical and anesthetic techniques in Procambarus clarkii maintained in a biomedical research setting were performed. (researchsquare.com)
Found2
- This study provides a useful guide to the normal cytological appearance of the canine brain, which was found to show similarities with the normal cytological appearance of the human brain. (gla.ac.uk)
- A semiquantitative centrifugation technique, developed for cytological purposes, was found to be the most sensitive. (bmj.com)
Topic1
- Did you mean topic:"cytologically techniques" ? (nshealth.ca)
Method1
- Three commonly used sectioning method are freehand section technique, paraffin method, and celloidin method. (wikipedia.org)
Glass slide1
- This technique is to place the material on the glass slide and remove it with the scalpel or to dissect needle, then add a drop of dye solution. (wikipedia.org)
Review1
- This review focuses on cytological clues as well as ancillary techniques that may be useful to the practicing cytopathologist. (sun.ac.za)
Special1
- Using a special profiling technique, scientists at Princeton have determined the mechanism of action of a potent antibiotic, known as tropodithietic acid (TDA), leading them to uncover its hidden ability as a potential anticancer agent. (princeton.edu)
Management1
- Introduction to common reproductive management techniques of equine reproduction including natural and artificial insemination. (dcccd.edu)
