A species of parasitic protozoa that infects humans and most domestic mammals. Its oocysts measure five microns in diameter. These organisms exhibit alternating cycles of sexual and asexual reproduction.
A genus of coccidian parasites of the family CRYPTOSPORIDIIDAE, found in the intestinal epithelium of many vertebrates including humans.
Intestinal infection with organisms of the genus CRYPTOSPORIDIUM. It occurs in both animals and humans. Symptoms include severe DIARRHEA.
Zygote-containing cysts of sporozoan protozoa. Further development in an oocyst produces small individual infective organisms called SPOROZOITES. Then, depending on the genus, the entire oocyst is called a sporocyst or the oocyst contains multiple sporocysts encapsulating the sporozoites.
A subclass of protozoans commonly parasitic in the epithelial cells of the intestinal tract but also found in the liver and other organs. Its organisms are found in both vertebrates and higher invertebrates and comprise two orders: EIMERIIDA and EUCOCCIDIIDA.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
A genus of flagellate intestinal EUKARYOTES parasitic in various vertebrates, including humans. Characteristics include the presence of four pairs of flagella arising from a complicated system of axonemes and cysts that are ellipsoidal to ovoidal in shape.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA LAMBLIA. It is spread via contaminated food and water and by direct person-to-person contact.
A species of parasitic EUKARYOTES that attaches itself to the intestinal mucosa and feeds on mucous secretions. The organism is roughly pear-shaped and motility is somewhat erratic, with a slow oscillation about the long axis.
The study of parasites and PARASITIC DISEASES.
Agents useful in the treatment or prevention of COCCIDIOSIS in man or animals.
Determination of parasite eggs in feces.
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Infections with unicellular organisms formerly members of the subkingdom Protozoa.
Means or process of supplying water (as for a community) usually including reservoirs, tunnels, and pipelines and often the watershed from which the water is ultimately drawn. (Webster, 3d ed)
An oligosaccharide antibiotic produced by various STREPTOMYCES.
The product of meiotic division of zygotes in parasitic protozoa comprising haploid cells. These infective cells invade the host and undergo asexual reproduction producing MEROZOITES (or other forms) and ultimately gametocytes.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Proteins found in any species of protozoan.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
A phylum of unicellular parasitic EUKARYOTES characterized by the presence of complex apical organelles generally consisting of a conoid that aids in penetrating host cells, rhoptries that possibly secrete a proteolytic enzyme, and subpellicular microtubules that may be related to motility.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Any of several processes in which undesirable impurities in water are removed or neutralized; for example, chlorination, filtration, primary treatment, ion exchange, and distillation. It includes treatment of WASTE WATER to provide potable and hygienic water in a controlled or closed environment as well as provision of public drinking water supplies.
A bacteria isolated from normal skin, intestinal contents, wounds, blood, pus, and soft tissue abscesses. It is a common contaminant of clinical specimens, presumably from the skin of patients or attendants.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Rendering pathogens harmless through the use of heat, antiseptics, antibacterial agents, etc.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The functional hereditary units of protozoa.
Substances that are destructive to protozoans.
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
Agents which are destructive to amebae, especially the parasitic species causing AMEBIASIS in man and animal.
Infections of the INTESTINES with PARASITES, commonly involving PARASITIC WORMS. Infections with roundworms (NEMATODE INFECTIONS) and tapeworms (CESTODE INFECTIONS) are also known as HELMINTHIASIS.
Drugs used to treat or prevent parasitic infections.
The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.
The relationship between an invertebrate and another organism (the host), one of which lives at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
An enzyme that catalyzes the dehydrogenation of inosine 5'-phosphate to xanthosine 5'-phosphate in the presence of NAD. EC 1.1.1.205.
Contamination of bodies of water (such as LAKES; RIVERS; SEAS; and GROUNDWATER.)
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
A genus of protozoan parasites found in the intestines of birds, amphibians, reptiles, and mammals, including man. The oocysts produce two sporocysts, each with four sporozoites. Many species are parasitic in wild and domestic animals.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The presence of parasites in food and food products. For the presence of bacteria, viruses, and fungi in food, FOOD MICROBIOLOGY is available.
Refuse liquid or waste matter carried off by sewers.
Diseases of non-human animals that may be transmitted to HUMANS or may be transmitted from humans to non-human animals.
Inorganic compounds that contain chlorine as an integral part of the molecule.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.

Cryptosporidium parvum sporozoite pellicle antigen recognized by a neutralizing monoclonal antibody is a beta-mannosylated glycolipid. (1/679)

The protozoan parasite Cryptosporidium parvum is an important cause of diarrhea in humans, calves, and other mammals worldwide. No approved vaccines or parasite-specific drugs are currently available for the control of cryptosporidiosis. To effectively immunize against C. parvum, identification and characterization of protective antigens are required. We previously identified CPS-500, a conserved, neutralization-sensitive antigen of C. parvum sporozoites and merozoites defined by monoclonal antibody 18.44. In the present study, the biochemical characteristics and subcellular location of CPS-500 were determined. CPS-500 was chloroform extractable and eluted with acetone and methanol in silicic acid chromatography, consistent with being a polar glycolipid. Following chloroform extraction and silicic acid chromatography, CPS-500 was isolated by high-pressure liquid chromatography for glycosyl analysis, which indicated the presence of mannose and inositol. To identify which component of CPS-500 comprised the neutralization-sensitive epitope recognized by 18.44, the ability of the monoclonal antibody to bind CPS-500 treated with proteases, or with alpha- or beta-glycosidases, was determined. Monoclonal antibody 18.44 did not bind antigen treated with beta-D-mannosidase but did bind antigen treated with alpha-D-mannosidase, other alpha- or beta-glycosidases, or a panel of proteases. These data indicated that the target epitope was dependent on terminal beta-D-mannopyranosyl residues. By immunoelectron microscopy, 18.44 binding was localized to the pellicle and an intracytoplasmic tubulovesicular network in sporozoites. Monoclonal antibody 18.44 also bound to antigen deposited and released onto substrate over the course travelled by gliding sporozoites and merozoites. Surface localization, adhesion and release during locomotion, and neutralization sensitivity suggest that CPS-500 may be involved in motility and invasion processes of the infective zoite stages.  (+info)

Cytokine profile induced by Cryptosporidium antigen in peripheral blood mononuclear cells from immunocompetent and immunosuppressed persons with cryptosporidiosis. (2/679)

The proliferative response of peripheral blood mononuclear cells (PBMC) to a crude extract from Cryptosporidium parvum (CCE) was studied in persons who acquired cryptosporidiosis in the same outbreak (15 immunocompetent subjects with prior cryptosporidiosis and 22 human immunodeficiency virus [HIV]-positive persons with various levels of immunosuppression and active cryptosporidiosis) and in individual patients (8 HIV-positive patients with active cryptosporidiosis and 15 HIV-positive persons without history of cryptosporidiosis). PBMC from HIV-positive persons showed less proliferation to CCE and mitogens than did PBMC from immunocompetent subjects with prior cryptosporidiosis, independent of CD4 cell count. In immunocompetent subjects, cytokine gene expression was consistent with cytokine production, whereas in HIV-positive subjects it was not. The production of interferon-gamma in CCE-stimulated PBMC from both immunocompetent and HIV-positive subjects with cryptosporidiosis and the lack of interferon-gamma in CCE-stimulated PBMC from HIV-positive subjects without cryptosporidiosis indicate that C. parvum mainly induces a Th1 response.  (+info)

Characterization of an immunogenic glycocalyx on the surfaces of Cryptosporidium parvum oocysts and sporozoites. (3/679)

Ruthenium red staining of Cryptosporidium parvum oocysts revealed the presence of a carbohydrate matrix on their outer bilayers that is characteristic of a glycocalyx. Surface labeling of intact oocysts identified material of high molecular weight (>10(6)) that reacted positively with sera from cryptosporidium-infected patients and with immunoglobulin A monoclonal antibodies.  (+info)

beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability. (4/679)

Determining the viability of waterborne Cryptosporidium parvum oocysts remains a technical challenge. rRNA and mRNA were evaluated in a reverse transcription (RT)-PCR assay as potential markers of oocyst viability. The rationale for this approach is the rapid turnover and postmortem decay of cellular RNA. The beta-tubulin mRNA and an anonymous mRNA transcript were chosen as potential markers because they are the only mRNA species in C. parvum known to possess introns. This feature facilitated the distinction between genuine RT-PCR products and PCR products originating from copurifying DNA. Prolonged incubation at room temperature of initially viable oocysts resulted in a gradual decrease in mRNA levels, which correlated with the loss of oocyst infectivity to neonatal mice. In contrast, oocysts stored at 4 degrees C for over 39 weeks maintained their infectivity and displayed no decrease in the level of beta-tubulin RT-PCR product. The postmortem decay of two mRNA species demonstrates that RT-PCR analysis can provide information on the viability of C. parvum oocysts. The methodological similarity between PCR detection and RT-PCR viability analysis could facilitate the development of a combined detection and viability assay.  (+info)

The Cryptosporidium "mouse" genotype is conserved across geographic areas. (5/679)

A 298-bp region of the Cryptosporidium parvum 18S rRNA gene and a 390-bp region of the acetyl coenzyme A synthetase gene were sequenced for a range of Cryptosporidium isolates from wild house mice (Mus domesticus), a bat (Myotus adversus), and cattle from different geographical areas. Previous research has identified a distinct genotype, referred to as the "mouse"-derived Cryptosporidium genotype, common to isolates from Australian mice. Comparison of a wider range of Australian mouse isolates with United Kingdom and Spanish isolates from mice and cattle and also an Australian bat-derived Cryptosporidium isolate revealed that the "mouse" genotype is conserved across geographic areas. Mice are also susceptible to infection with the "cattle" Cryptosporidium genotype, which has important implications for their role as reservoirs of infection for humans and domestic animals.  (+info)

Detection by enzyme immunoassay of serum immunoglobulin G antibodies that recognize specific Cryptosporidium parvum antigens. (6/679)

Human infection with Cryptosporidium parvum usually elicits characteristic immunoglobulin G (IgG), IgA, and IgM antibody responses against two sporozoite surface antigens with apparent molecular masses of approximately 27 and 17 kDa. We have determined that these two antigens are actually complex families of related antigens. We have developed two new enzyme-linked immunosorbent assays (ELISAs) for the detection and quantitation of serum IgG antibodies against both antigens. The assays utilize a recombinant form of the 27-kDa antigen and a partially purified native fraction isolated from sonicated whole oocysts that contains 17-kDa antigen. An immunoblot assay previously developed in our laboratory served as the reference, or "gold standard," seroassay for the assessment of the new ELISAs. Positive responses with the recombinant-27-kDa-antigen ELISA were correlated with the immunoblot results for the 27-kDa antigen, with a sensitivity and specificity of 90 and 92%, respectively. Similarly, positive responses with the partially purified native-17-kDa-antigen ELISA correlated with the immunoblot results for the 17-kDa antigen, with a sensitivity and specificity of 90 and 94%, respectively. For both ELISAs the median IgG antibody levels for serum sets collected during outbreaks of waterborne C. parvum infection were at least 2.5-fold higher than the levels determined for a nonoutbreak set. Using the immunoblot as the "gold standard," the new ELISAs were more specific and, in the case of the 27-kDa-antigen ELISA, more sensitive than the crude oocyst antigen ELISA currently in use. These assays will be useful in future epidemiologic studies.  (+info)

Use of a sentinel system for field measurements of Cryptosporidium parvum oocyst inactivation in soil and animal waste. (7/679)

A small-volume sentinel chamber was developed to assess the effects of environmental stresses on survival of sucrose-Percoll-purified Cryptosporidium parvum oocysts in soil and animal wastes. Chambers were tested for their ability to equilibrate with external chemical and moisture conditions. Sentinel oocysts were then exposed to stresses of the external environment that affected their viability (potential infectivity), as indicated by results of a dye permeability assay. Preliminary laboratory experiments indicated that temperatures between 35 and 50 degrees C and decreases in soil water potential (-0.003 to -3.20 MPa) increased oocyst inactivation rates. The effects of two common animal waste management practices on oocyst survival were investigated on three dairy farms in Delaware County, N.Y., within the New York City watershed: (i) piling wastes from dairy youngstock (including neonatal calves) and (ii) spreading wastes as a soil amendment on an agricultural field. Sentinel containers filled with air-dried and sieved (2-mm mesh) youngstock waste or field soil were wetted and inoculated with 2 million oocysts in an aqueous suspension and then placed in waste piles on two different farms and in soil within a cropped field on one farm. Controls consisted of purified oocysts in either phosphate-buffered saline or distilled water contained in sealed microcentrifuge tubes. Two microdata loggers recorded the ambient temperature at each field site. Sentinel experiments were conducted during the fall and winter (1996 to 1997) and winter (1998). Sentinel containers and controls were removed at 2- to 4-week intervals, and oocysts were extracted and tested by the dye permeability assay. The proportions of potentially infective oocysts exposed to the soil and waste pile material decreased more rapidly than their counterpart controls exposed to buffer or water, indicating that factors other than temperature affected oocyst inactivation in the waste piles and soil. The effect of soil freeze-thaw cycles was evident in the large proportion of empty sentinel oocysts. The potentially infective sentinel oocysts were reduced to <1% while the proportions in controls did not decrease below 50% potentially infective during the first field experiment. Microscopic observations of empty oocyst fragments indicated that abrasive effects of soil particles were a factor in oocyst inactivation. A similar pattern was observed in a second field experiment at the same site.  (+info)

CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages. (8/679)

The intracellular parasite Cryptosporidium parvum develops inside a vacuole at the apex of its epithelial host cell. The developing parasite is separated from the host cell cytoplasm by a zone of attachment that consists of an extensively folded membranous structure known as the feeder organelle. It has been proposed that the feeder organelle is the site of regulation of transport of nutrients and drugs into the parasite. In this report, we localize an approximately 200-kDa integral membrane protein, CpABC, from Cryptosporidium parvum to the host-parasite boundary, possibly the feeder organelle. The predicted amino acid sequence of CpABC has significant structural similarity with the cystic fibrosis conductance regulator and the multidrug resistance protein subfamily of ATP-binding cassette proteins. This is an example of a parasite-encoded transport protein localized at the parasite-host interface of an intracellular protozoan.  (+info)

Cryptosporidium parvum is a species of protozoan parasite that causes the diarrheal disease cryptosporidiosis in humans and animals. It is found worldwide and is transmitted through the fecal-oral route, often through contaminated water or food. The parasite infects the epithelial cells of the gastrointestinal tract, leading to symptoms such as watery diarrhea, stomach cramps, nausea, and fever. It is particularly dangerous for people with weakened immune systems, such as those with HIV/AIDS or receiving immunosuppressive therapy. The parasite is highly resistant to chlorine-based disinfectants, making it difficult to eradicate from water supplies.

Cryptosporidium is a genus of protozoan parasites that can cause the diarrheal disease known as cryptosporidiosis in humans and animals. These microscopic pathogens infect the epithelial cells of the gastrointestinal tract, primarily in the small intestine, leading to symptoms such as watery diarrhea, stomach cramps, nausea, vomiting, fever, and dehydration.

Cryptosporidium parasites have a complex life cycle, including several developmental stages within host cells. They are protected by an outer shell called oocyst, which allows them to survive outside the host's body for extended periods, making them resistant to chlorine-based disinfectants commonly used in water treatment.

Transmission of Cryptosporidium occurs through the fecal-oral route, often via contaminated water or food, or direct contact with infected individuals or animals. People at higher risk for severe illness include young children, elderly people, pregnant women, and those with weakened immune systems due to HIV/AIDS, cancer treatment, or organ transplantation.

Preventive measures include proper hand hygiene, avoiding consumption of untreated water or raw fruits and vegetables likely to be contaminated, and practicing safe sex. For immunocompromised individuals, antiparasitic medications such as nitazoxanide may help reduce the severity and duration of symptoms.

Cryptosporidiosis is a diarrheal disease caused by microscopic parasites called Cryptosporidium. The parasites are found in the feces of infected animals and humans. People can become infected with Cryptosporidium by ingesting contaminated water or food, or by coming into contact with infected persons or animals.

The infection can cause a wide range of symptoms, including watery diarrhea, stomach cramps, nausea, vomiting, fever, and dehydration. In people with weakened immune systems, such as those with HIV/AIDS, the infection can be severe and even life-threatening.

Cryptosporidiosis is typically treated with increased fluid intake to prevent dehydration, and in some cases, medication may be prescribed to help manage symptoms. Good hygiene practices, such as washing hands thoroughly after using the bathroom or changing diapers, can help prevent the spread of Cryptosporidium.

An oocyst is a thick-walled, environmentally resistant spore-like structure produced by some protozoan parasites, such as Cryptosporidium and Cyclospora, during their life cycle. These oocysts can survive for long periods in the environment and can infect a host when ingested, leading to infection and disease. The term "oocyst" is specific to certain groups of protozoan parasites and should not be confused with other types of spores produced by fungi or bacteria.

Coccidia are a group of single-celled, microscopic parasites that belong to the phylum Apicomplexa. They are obligate intracellular parasites, which means they need to infect and live inside the cells of a host organism to survive and multiply. Coccidia are primarily found in animals, including mammals, birds, reptiles, and fish, but some species can also infect humans.

Coccidia are known to cause coccidiosis, a common intestinal disease that affects various animal species, including poultry, cattle, swine, sheep, goats, and pets such as cats and dogs. The disease is characterized by diarrhea, weight loss, dehydration, and sometimes death, particularly in young animals.

In humans, coccidia infection is usually caused by the species Cryptosporidium and Cyclospora. These parasites can infect the small intestine and cause watery diarrhea, stomach cramps, nausea, vomiting, fever, and weight loss. In immunocompromised individuals, such as those with HIV/AIDS or those undergoing chemotherapy, coccidia infections can be severe and life-threatening.

Coccidia are typically transmitted through the fecal-oral route, either by ingesting contaminated food or water or by direct contact with infected animals or their feces. Prevention measures include good hygiene practices, such as washing hands thoroughly after handling animals or using the restroom, avoiding drinking untreated water from sources that may be contaminated with animal feces, and practicing safe food handling and preparation.

There doesn't seem to be a specific medical definition for "DNA, protozoan" as it is simply a reference to the DNA found in protozoa. Protozoa are single-celled eukaryotic organisms that can be found in various environments such as soil, water, and the digestive tracts of animals.

Protozoan DNA refers to the genetic material present in these organisms. It is composed of nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which contain the instructions for the development, growth, and reproduction of the protozoan.

The DNA in protozoa, like in other organisms, is made up of two strands of nucleotides that coil together to form a double helix. The four nucleotide bases that make up protozoan DNA are adenine (A), thymine (T), guanine (G), and cytosine (C). These bases pair with each other to form the rungs of the DNA ladder, with A always pairing with T and G always pairing with C.

The genetic information stored in protozoan DNA is encoded in the sequence of these nucleotide bases. This information is used to synthesize proteins, which are essential for the structure and function of the organism's cells. Protozoan DNA also contains other types of genetic material, such as regulatory sequences that control gene expression and repetitive elements with no known function.

Understanding the DNA of protozoa is important for studying their biology, evolution, and pathogenicity. It can help researchers develop new treatments for protozoan diseases and gain insights into the fundamental principles of genetics and cellular function.

Giardia is a genus of microscopic parasitic flagellates that cause giardiasis, a type of diarrheal disease. The most common species to infect humans is Giardia intestinalis (also known as Giardia lamblia or Giardia duodenalis). These microscopic parasites are found worldwide, particularly in areas with poor sanitation and unsafe water.

Giardia exists in two forms: the trophozoite, which is the actively feeding form that multiplies in the small intestine, and the cyst, which is the infective stage that is passed in feces and can survive outside the body for long periods under appropriate conditions. Infection occurs when a person ingests contaminated water or food, or comes into direct contact with an infected person's feces.

Once inside the body, the cysts transform into trophozoites, which attach to the lining of the small intestine and disrupt the normal function of the digestive system, leading to symptoms such as diarrhea, stomach cramps, nausea, dehydration, and weight loss. In some cases, giardiasis can cause long-term health problems, particularly in children, including malnutrition and developmental delays.

Preventing the spread of Giardia involves maintaining good hygiene practices, such as washing hands thoroughly after using the toilet or changing diapers, avoiding contaminated water sources, and practicing safe food handling and preparation. In cases where infection occurs, medication is usually effective in treating the illness.

Feces are the solid or semisolid remains of food that could not be digested or absorbed in the small intestine, along with bacteria and other waste products. After being stored in the colon, feces are eliminated from the body through the rectum and anus during defecation. Feces can vary in color, consistency, and odor depending on a person's diet, health status, and other factors.

18S rRNA (ribosomal RNA) is the smaller subunit of the eukaryotic ribosome, which is the cellular organelle responsible for protein synthesis. The "18S" refers to the sedimentation coefficient of this rRNA molecule, which is a measure of its rate of sedimentation in a centrifuge and is expressed in Svedberg units (S).

The 18S rRNA is a component of the 40S subunit of the ribosome, and it plays a crucial role in the decoding of messenger RNA (mRNA) during protein synthesis. Specifically, the 18S rRNA helps to form the structure of the ribosome and contains several conserved regions that are involved in binding to mRNA and guiding the movement of transfer RNAs (tRNAs) during translation.

The 18S rRNA is also a commonly used molecular marker for evolutionary studies, as its sequence is highly conserved across different species and can be used to infer phylogenetic relationships between organisms. Additionally, the analysis of 18S rRNA gene sequences has been widely used in various fields such as ecology, environmental science, and medicine to study biodiversity, biogeography, and infectious diseases.

Giardiasis is a digestive infection caused by the microscopic parasite Giardia intestinalis, also known as Giardia lamblia or Giardia duodenalis. The parasite is found worldwide, especially in areas with poor sanitation and unsafe water.

The infection typically occurs after ingesting contaminated water, food, or surfaces that have been exposed to fecal matter containing the cyst form of the parasite. Once inside the body, the cysts transform into trophozoites, which attach to the lining of the small intestine and cause symptoms such as diarrhea, stomach cramps, nausea, dehydration, and greasy stools that may float due to excess fat.

In some cases, giardiasis can lead to lactose intolerance and malabsorption of nutrients, resulting in weight loss and vitamin deficiencies. The infection is usually diagnosed through a stool sample test and treated with antibiotics such as metronidazole or tinidazole. Preventive measures include practicing good hygiene, avoiding contaminated water and food, and washing hands regularly.

"Giardia lamblia," also known as "Giardia duodenalis" or "Giardia intestinalis," is a species of microscopic parasitic protozoan that colonizes and reproduces in the small intestine of various vertebrates, including humans. It is the most common cause of human giardiasis, a diarrheal disease. The trophozoite (feeding form) of Giardia lamblia has a distinctive tear-drop shape and possesses flagella for locomotion. It attaches to the intestinal epithelium, disrupting the normal function of the small intestine and leading to various gastrointestinal symptoms such as diarrhea, stomach cramps, nausea, and dehydration. Giardia lamblia is typically transmitted through the fecal-oral route, often via contaminated food or water.

Parasitology is a branch of biology that deals with the study of parasites, their life cycles, the relationship between parasites and their hosts, the transmission of parasitic diseases, and the development of methods for their control and elimination. It involves understanding various types of parasites including protozoa, helminths, and arthropods that can infect humans, animals, and plants. Parasitologists also study the evolution, genetics, biochemistry, and ecology of parasites to develop effective strategies for their diagnosis, treatment, and prevention.

Coccidiostats are a type of medication used to prevent and treat coccidiosis, which is an infection caused by protozoan parasites of the genus Coccidia. These medications work by inhibiting the growth and reproduction of the parasites in the gastrointestinal tract of animals, particularly poultry and livestock.

Coccidiostats are commonly added to animal feed to prevent infection and reduce the spread of coccidiosis within a flock or herd. They can also be used to treat active infections, often in combination with other medications. Common examples of coccidiostats include sulfaquinoxaline, monensin, and lasalocid.

It's important to note that the use of coccidiostats in food-producing animals is regulated by government agencies such as the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) to ensure their safe use and to minimize the risk of residues in animal products.

A "Parasite Egg Count" is a laboratory measurement used to estimate the number of parasitic eggs present in a fecal sample. It is commonly used in veterinary and human medicine to diagnose and monitor parasitic infections, such as those caused by roundworms, hookworms, tapeworms, and other intestinal helminths (parasitic worms).

The most common method for measuring parasite egg counts is the McMaster technique. This involves mixing a known volume of feces with a flotation solution, which causes the eggs to float to the top of the mixture. A small sample of this mixture is then placed on a special counting chamber and examined under a microscope. The number of eggs present in the sample is then multiplied by a dilution factor to estimate the total number of eggs per gram (EPG) of feces.

Parasite egg counts can provide valuable information about the severity of an infection, as well as the effectiveness of treatment. However, it is important to note that not all parasitic infections produce visible eggs in the feces, and some parasites may only shed eggs intermittently. Therefore, a negative egg count does not always rule out the presence of a parasitic infection.

I'd be happy to help! However, I believe there might be a slight confusion in your question. RNA stands for Ribonucleic Acid, which is a type of nucleic acid involved in various biological roles in the coding, decoding, regulation, and expression of genes. On the other hand, protozoan refers to a unicellular organism from the kingdom Protista, which includes a wide variety of simple eukaryotic organisms such as amoebas, paramecia, and plasmodium (the malaria-causing parasite).

There isn't a specific medical definition for "RNA, protozoan" since RNA is a molecule present in all living cells, including human cells, and protozoans are a group of organisms. However, I can tell you that RNA plays crucial roles in protozoan biology, such as acting as a messenger between DNA and ribosomes during protein synthesis or regulating gene expression.

If you have any further questions or need more specific information about RNA in protozoans, please let me know!

Protozoan infections are diseases caused by microscopic, single-celled organisms known as protozoa. These parasites can enter the human body through contaminated food, water, or contact with an infected person or animal. Once inside the body, they can multiply and cause a range of symptoms depending on the type of protozoan and where it infects in the body. Some common protozoan infections include malaria, giardiasis, amoebiasis, and toxoplasmosis. Symptoms can vary widely but may include diarrhea, abdominal pain, fever, fatigue, and skin rashes. Treatment typically involves the use of antiprotozoal medications to kill the parasites and alleviate symptoms.

I'm sorry for any confusion, but "water supply" is not a medical term per se. It is a general term used to describe the system or arrangement providing water for consumption or use, such as a public water supply system or a private well. However, in a medical context, it could refer to the source of water used in a healthcare facility for drinking, cooking, cleaning, and patient care, which must meet certain quality standards to prevent infection and ensure safety.

Paromomycin is an antiprotozoal medication, which belongs to the class of aminoglycoside antibiotics. It is primarily used to treat various intestinal infectious diseases caused by protozoa, such as amebiasis (an infection caused by Entamoeba histolytica) and giardiasis (an infection caused by Giardia lamblia). Paromomycin works by inhibiting the protein synthesis in the parasites, leading to their death. It is not typically used to treat bacterial infections in humans, as other aminoglycosides are.

It's important to note that paromomycin has limited systemic absorption and is primarily active within the gastrointestinal tract when taken orally. This makes it a valuable option for treating intestinal parasitic infections without causing significant harm to the beneficial bacteria in the gut or systemically affecting other organs.

Paromomycin is also used in veterinary medicine to treat various protozoal infections in animals, including leishmaniasis in dogs. The medication is available in different forms, such as tablets, capsules, and powder for oral suspension. As with any medication, paromomycin should be taken under the supervision of a healthcare professional, and its use may be subject to specific dosage, frequency, and duration guidelines.

Sporozoites are a stage in the life cycle of certain parasitic protozoans, including Plasmodium species that cause malaria. They are infective forms that result from the sporulation of oocysts, which are produced in the vector's midgut after the ingestion of gametocytes during a blood meal.

Once mature, sporozoites are released from the oocyst and migrate to the salivary glands of the vector, where they get injected into the host during subsequent feedings. In the host, sporozoites infect liver cells, multiply within them, and eventually rupture the cells, releasing merozoites that invade red blood cells and initiate the erythrocytic stage of the parasite's life cycle.

Sporozoites are typically highly motile and possess a unique gliding motility, which enables them to traverse various host tissues during their invasion process. This invasive ability is facilitated by an actin-myosin motor system and secretory organelles called micronemes and rhoptries, which release adhesive proteins that interact with host cell receptors.

In summary, sporozoites are a crucial stage in the life cycle of Plasmodium parasites, serving as the infective forms responsible for transmitting malaria between hosts via an insect vector.

Antibodies, protozoan, refer to the immune system's response to an infection caused by a protozoan organism. Protozoa are single-celled microorganisms that can cause various diseases in humans, such as malaria, giardiasis, and toxoplasmosis.

When the body is infected with a protozoan, the immune system responds by producing specific proteins called antibodies. Antibodies are produced by a type of white blood cell called a B-cell, and they recognize and bind to specific antigens on the surface of the protozoan organism.

There are five main types of antibodies: IgA, IgD, IgE, IgG, and IgM. Each type of antibody has a different role in the immune response. For example, IgG is the most common type of antibody and provides long-term immunity to previously encountered pathogens. IgM is the first antibody produced in response to an infection and is important for activating the complement system, which helps to destroy the protozoan organism.

Overall, the production of antibodies against protozoan organisms is a critical part of the immune response and helps to protect the body from further infection.

"Cattle" is a term used in the agricultural and veterinary fields to refer to domesticated animals of the genus *Bos*, primarily *Bos taurus* (European cattle) and *Bos indicus* (Zebu). These animals are often raised for meat, milk, leather, and labor. They are also known as bovines or cows (for females), bulls (intact males), and steers/bullocks (castrated males). However, in a strict medical definition, "cattle" does not apply to humans or other animals.

Diarrhea is a condition in which an individual experiences loose, watery stools frequently, often exceeding three times a day. It can be acute, lasting for several days, or chronic, persisting for weeks or even months. Diarrhea can result from various factors, including viral, bacterial, or parasitic infections, food intolerances, medications, and underlying medical conditions such as inflammatory bowel disease or irritable bowel syndrome. Dehydration is a potential complication of diarrhea, particularly in severe cases or in vulnerable populations like young children and the elderly.

I'm sorry for any confusion, but "Protozoan Proteins" is not a specific medical or scientific term. Protozoa are single-celled eukaryotic organisms, and proteins are large biological molecules consisting of one or more chains of amino acid residues. Therefore, "Protozoan Proteins" generally refers to the various types of proteins found in protozoa.

However, if you're looking for information about proteins specific to certain protozoan parasites with medical relevance (such as Plasmodium falciparum, which causes malaria), I would be happy to help! Please provide more context or specify the particular protozoan of interest.

Cattle diseases are a range of health conditions that affect cattle, which include but are not limited to:

1. Bovine Respiratory Disease (BRD): Also known as "shipping fever," BRD is a common respiratory illness in feedlot cattle that can be caused by several viruses and bacteria.
2. Bovine Viral Diarrhea (BVD): A viral disease that can cause a variety of symptoms, including diarrhea, fever, and reproductive issues.
3. Johne's Disease: A chronic wasting disease caused by the bacterium Mycobacterium avium subspecies paratuberculosis. It primarily affects the intestines and can cause severe diarrhea and weight loss.
4. Digital Dermatitis: Also known as "hairy heel warts," this is a highly contagious skin disease that affects the feet of cattle, causing lameness and decreased productivity.
5. Infectious Bovine Keratoconjunctivitis (IBK): Also known as "pinkeye," IBK is a common and contagious eye infection in cattle that can cause blindness if left untreated.
6. Salmonella: A group of bacteria that can cause severe gastrointestinal illness in cattle, including diarrhea, dehydration, and septicemia.
7. Leptospirosis: A bacterial disease that can cause a wide range of symptoms in cattle, including abortion, stillbirths, and kidney damage.
8. Blackleg: A highly fatal bacterial disease that causes rapid death in young cattle. It is caused by Clostridium chauvoei and vaccination is recommended for prevention.
9. Anthrax: A serious infectious disease caused by the bacterium Bacillus anthracis. Cattle can become infected by ingesting spores found in contaminated soil, feed or water.
10. Foot-and-Mouth Disease (FMD): A highly contagious viral disease that affects cloven-hooved animals, including cattle. It is characterized by fever and blisters on the feet, mouth, and teats. FMD is not a threat to human health but can have serious economic consequences for the livestock industry.

It's important to note that many of these diseases can be prevented or controlled through good management practices, such as vaccination, biosecurity measures, and proper nutrition. Regular veterinary care and monitoring are also crucial for early detection and treatment of any potential health issues in your herd.

Apicomplexa is a phylum of single-celled, parasitic organisms that includes several medically important genera, such as Plasmodium (which causes malaria), Toxoplasma (which causes toxoplasmosis), and Cryptosporidium (which causes cryptosporidiosis). These organisms are characterized by the presence of a unique apical complex, which is a group of specialized structures at one end of the cell that are used during invasion and infection of host cells. They have a complex life cycle involving multiple stages, including sexual and asexual reproduction, often in different hosts. Many Apicomplexa are intracellular parasites, meaning they live and multiply inside the cells of their hosts.

Medical definitions of water generally describe it as a colorless, odorless, tasteless liquid that is essential for all forms of life. It is a universal solvent, making it an excellent medium for transporting nutrients and waste products within the body. Water constitutes about 50-70% of an individual's body weight, depending on factors such as age, sex, and muscle mass.

In medical terms, water has several important functions in the human body:

1. Regulation of body temperature through perspiration and respiration.
2. Acting as a lubricant for joints and tissues.
3. Facilitating digestion by helping to break down food particles.
4. Transporting nutrients, oxygen, and waste products throughout the body.
5. Helping to maintain healthy skin and mucous membranes.
6. Assisting in the regulation of various bodily functions, such as blood pressure and heart rate.

Dehydration can occur when an individual does not consume enough water or loses too much fluid due to illness, exercise, or other factors. This can lead to a variety of symptoms, including dry mouth, fatigue, dizziness, and confusion. Severe dehydration can be life-threatening if left untreated.

Water purification is the process of removing or reducing contaminants in water to make it safe and suitable for specific uses, such as drinking, cooking, irrigation, or medical purposes. This is typically achieved through physical, chemical, or biological methods, or a combination thereof. The goal is to eliminate or reduce harmful substances like bacteria, viruses, parasites, heavy metals, pesticides, and other pollutants that can cause illness or negatively impact human health, aquatic life, or the environment.

The specific purification methods used may vary depending on the nature of the contaminants and the desired level of purity for the intended use. Common techniques include filtration (using various types of filters like activated carbon, ceramic, or reverse osmosis), disinfection (using chemicals like chlorine or UV light to kill microorganisms), sedimentation (allowing particles to settle and be removed), and distillation (heating water to create steam, which is then condensed back into pure water).

Propionibacterium acnes is a gram-positive, rod-shaped bacterium that naturally colonizes the skin, predominantly in areas with a high density of sebaceous glands such as the face, back, and chest. It is part of the normal skin flora but can contribute to the development of acne vulgaris when it proliferates excessively and clogs the pilosebaceous units (hair follicles).

The bacterium metabolizes sebum, producing propionic acid and other short-chain fatty acids as byproducts. In acne, these byproducts can cause an inflammatory response in the skin, leading to the formation of papules, pustules, and nodules. Propionibacterium acnes has also been implicated in various other skin conditions and occasionally in opportunistic infections in other parts of the body, particularly in immunocompromised individuals or following surgical procedures.

Antigens are substances (usually proteins) found on the surface of cells, or viruses, that can be recognized by the immune system and stimulate an immune response. In the context of protozoa, antigens refer to the specific proteins or other molecules found on the surface of these single-celled organisms that can trigger an immune response in a host organism.

Protozoa are a group of microscopic eukaryotic organisms that include a diverse range of species, some of which can cause diseases in humans and animals. When a protozoan infects a host, the host's immune system recognizes the protozoan antigens as foreign and mounts an immune response to eliminate the infection. This response involves the activation of various types of immune cells, such as T-cells and B-cells, which recognize and target the protozoan antigens.

Understanding the nature of protozoan antigens is important for developing vaccines and other immunotherapies to prevent or treat protozoan infections. For example, researchers have identified specific antigens on the surface of the malaria parasite that are recognized by the human immune system and have used this information to develop vaccine candidates. However, many protozoan infections remain difficult to prevent or treat, and further research is needed to identify new targets for vaccines and therapies.

Disinfection is the process of eliminating or reducing harmful microorganisms from inanimate objects and surfaces through the use of chemicals, heat, or other methods. The goal of disinfection is to reduce the number of pathogens to a level that is considered safe for human health. Disinfection is an important step in preventing the spread of infectious diseases in healthcare settings, food processing facilities, and other environments where there is a risk of infection transmission.

It's important to note that disinfection is not the same as sterilization, which is the complete elimination of all microorganisms, including spores. Disinfection is generally less effective than sterilization but is often sufficient for most non-critical surfaces and objects. The choice between disinfection and sterilization depends on the level of risk associated with the item or surface being treated and the intended use of that item or surface.

Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify specific regions of DNA. It enables the production of thousands to millions of copies of a particular DNA sequence in a rapid and efficient manner, making it an essential tool in various fields such as molecular biology, medical diagnostics, forensic science, and research.

The PCR process involves repeated cycles of heating and cooling to separate the DNA strands, allow primers (short sequences of single-stranded DNA) to attach to the target regions, and extend these primers using an enzyme called Taq polymerase, resulting in the exponential amplification of the desired DNA segment.

In a medical context, PCR is often used for detecting and quantifying specific pathogens (viruses, bacteria, fungi, or parasites) in clinical samples, identifying genetic mutations or polymorphisms associated with diseases, monitoring disease progression, and evaluating treatment effectiveness.

The Fluorescent Antibody Technique (FAT), Direct is a type of immunofluorescence assay used in laboratory diagnostic tests. It is a method for identifying and locating specific antigens in cells or tissues by using fluorescent-labeled antibodies that directly bind to the target antigen.

In this technique, a sample (such as a tissue section or cell smear) is prepared and then treated with a fluorescently labeled primary antibody that specifically binds to the antigen of interest. After washing away unbound antibodies, the sample is examined under a fluorescence microscope. If the antigen is present in the sample, it will be visible as distinct areas of fluorescence, allowing for the direct visualization and localization of the antigen within the cells or tissues.

Direct FAT is commonly used in diagnostic laboratories to identify and diagnose various infectious diseases, including bacterial, viral, and fungal infections. It can also be used to detect specific proteins or antigens in research and clinical settings.

Genes in protozoa refer to the hereditary units of these single-celled organisms that carry genetic information necessary for their growth, development, and reproduction. These genes are made up of DNA (deoxyribonucleic acid) molecules, which contain sequences of nucleotide bases that code for specific proteins or RNA molecules. Protozoan genes are responsible for various functions, such as metabolism, response to environmental stimuli, and reproduction.

It is important to note that the study of protozoan genes has contributed significantly to our understanding of genetics and evolution, particularly in areas such as molecular biology, cell biology, and genomics. However, there is still much to be learned about the genetic diversity and complexity of these organisms, which continue to be an active area of research.

Antiprotozoal agents are a type of medication used to treat protozoal infections, which are infections caused by microscopic single-celled organisms called protozoa. These agents work by either killing the protozoa or inhibiting their growth and reproduction. They can be administered through various routes, including oral, topical, and intravenous, depending on the type of infection and the severity of the illness.

Examples of antiprotozoal agents include:

* Metronidazole, tinidazole, and nitazoxanide for treating infections caused by Giardia lamblia and Entamoeba histolytica.
* Atovaquone, clindamycin, and pyrimethamine-sulfadoxine for treating malaria caused by Plasmodium falciparum or other Plasmodium species.
* Pentamidine and suramin for treating African trypanosomiasis (sleeping sickness) caused by Trypanosoma brucei gambiense or T. b. rhodesiense.
* Nitroimidazoles, such as benznidazole and nifurtimox, for treating Chagas disease caused by Trypanosoma cruzi.
* Sodium stibogluconate and paromomycin for treating leishmaniasis caused by Leishmania species.

Antiprotozoal agents can have side effects, ranging from mild to severe, depending on the drug and the individual patient's response. It is essential to follow the prescribing physician's instructions carefully when taking these medications and report any adverse reactions promptly.

Immunomagnetic separation (IMS) is a medical diagnostic technique that combines the specificity of antibodies with the magnetic properties of nanoparticles to isolate and concentrate target cells or molecules from a sample. This method is widely used in research and clinical laboratories for the detection and analysis of various biological components, including bacteria, viruses, parasites, and tumor cells.

The process involves the use of magnetic beads coated with specific antibodies that bind to the target cells or molecules. Once bound, an external magnetic field is applied to separate the labeled cells or molecules from the unbound components in the sample. The isolated targets can then be washed, concentrated, and further analyzed using various methods such as polymerase chain reaction (PCR), flow cytometry, or microscopy.

IMS offers several advantages over traditional separation techniques, including high specificity, gentle handling of cells, minimal sample manipulation, and the ability to process large volumes of samples. These features make IMS a valuable tool in various fields, such as immunology, microbiology, hematology, oncology, and molecular biology.

Amebicides are medications that are used to treat infections caused by amebae, which are single-celled microorganisms. One common ameba that can cause infection in humans is Entamoeba histolytica, which can lead to a condition called amebiasis. Amebicides work by killing or inhibiting the growth of the amebae. Some examples of amebicides include metronidazole, tinidazole, and chloroquine. It's important to note that these medications should only be used under the guidance of a healthcare professional, as they can have side effects and may interact with other medications.

Parasitic intestinal diseases are disorders caused by microscopic parasites that invade the gastrointestinal tract, specifically the small intestine. These parasites include protozoa (single-celled organisms) and helminths (parasitic worms). The most common protozoan parasites that cause intestinal disease are Giardia lamblia, Cryptosporidium parvum, and Entamoeba histolytica. Common helminthic parasites include roundworms (Ascaris lumbricoides), tapeworms (Taenia saginata and Taenia solium), hookworms (Ancylostoma duodenale and Necator americanus), and pinworms (Enterobius vermicularis).

Parasitic intestinal diseases can cause a variety of symptoms, including diarrhea, abdominal pain, bloating, nausea, vomiting, fatigue, and weight loss. The severity and duration of the symptoms depend on the type of parasite, the number of organisms present, and the immune status of the host.

Transmission of these parasites can occur through various routes, including contaminated food and water, person-to-person contact, and contact with contaminated soil or feces. Preventive measures include practicing good hygiene, washing hands thoroughly after using the toilet and before handling food, cooking food thoroughly, and avoiding consumption of raw or undercooked meat, poultry, or seafood.

Treatment of parasitic intestinal diseases typically involves the use of antiparasitic medications that target the specific parasite causing the infection. In some cases, supportive care such as fluid replacement and symptom management may also be necessary.

Antiparasitic agents are a type of medication used to treat parasitic infections. These agents include a wide range of drugs that work to destroy, inhibit the growth of, or otherwise eliminate parasites from the body. Parasites are organisms that live on or inside a host and derive nutrients at the host's expense.

Antiparasitic agents can be divided into several categories based on the type of parasite they target. Some examples include:

* Antimalarial agents: These drugs are used to treat and prevent malaria, which is caused by a parasite that is transmitted through the bites of infected mosquitoes.
* Antiprotozoal agents: These drugs are used to treat infections caused by protozoa, which are single-celled organisms that can cause diseases such as giardiasis, amoebic dysentery, and sleeping sickness.
* Antihelminthic agents: These drugs are used to treat infections caused by helminths, which are parasitic worms that can infect various organs of the body, including the intestines, lungs, and skin. Examples include roundworms, tapeworms, and flukes.

Antiparasitic agents work in different ways to target parasites. Some disrupt the parasite's metabolism or interfere with its ability to reproduce. Others damage the parasite's membrane or exoskeleton, leading to its death. The specific mechanism of action depends on the type of antiparasitic agent and the parasite it is targeting.

It is important to note that while antiparasitic agents can be effective in treating parasitic infections, they can also have side effects and potential risks. Therefore, it is essential to consult with a healthcare provider before starting any antiparasitic medication to ensure safe and appropriate use.

A protozoan genome refers to the complete set of genetic material or DNA present in a protozoan organism. Protozoa are single-celled eukaryotic microorganisms that lack cell walls and have diverse morphology and nutrition modes. The genome of a protozoan includes all the genes that code for proteins, as well as non-coding DNA sequences that regulate gene expression and other cellular processes.

The size and complexity of protozoan genomes can vary widely depending on the species. Some protozoa have small genomes with only a few thousand genes, while others have larger genomes with tens of thousands of genes or more. The genome sequencing of various protozoan species has provided valuable insights into their evolutionary history, biology, and potential as model organisms for studying eukaryotic cellular processes.

It is worth noting that the study of protozoan genomics is still an active area of research, and new discoveries are continually being made about the genetic diversity and complexity of these fascinating microorganisms.

Host-parasite interactions refer to the relationship between a parasitic organism (the parasite) and its host, which can be an animal, plant, or human body. The parasite lives on or inside the host and derives nutrients from it, often causing harm in the process. This interaction can range from relatively benign to severe, depending on various factors such as the species of the parasite, the immune response of the host, and the duration of infection.

The host-parasite relationship is often categorized based on the degree of harm caused to the host. Parasites that cause little to no harm are called commensals, while those that cause significant damage or disease are called parasitic pathogens. Some parasites can even manipulate their hosts' behavior and physiology to enhance their own survival and reproduction, leading to complex interactions between the two organisms.

Understanding host-parasite interactions is crucial for developing effective strategies to prevent and treat parasitic infections, as well as for understanding the ecological relationships between different species in natural ecosystems.

Water microbiology is not a formal medical term, but rather a branch of microbiology that deals with the study of microorganisms found in water. It involves the identification, enumeration, and characterization of bacteria, viruses, parasites, and other microscopic organisms present in water sources such as lakes, rivers, oceans, groundwater, drinking water, and wastewater.

In a medical context, water microbiology is relevant to public health because it helps to assess the safety of water supplies for human consumption and recreational activities. It also plays a critical role in understanding and preventing waterborne diseases caused by pathogenic microorganisms that can lead to illnesses such as diarrhea, skin infections, and respiratory problems.

Water microbiologists use various techniques to study water microorganisms, including culturing, microscopy, genetic analysis, and biochemical tests. They also investigate the ecology of these organisms, their interactions with other species, and their response to environmental factors such as temperature, pH, and nutrient availability.

Overall, water microbiology is a vital field that helps ensure the safety of our water resources and protects public health.

Restriction Fragment Length Polymorphism (RFLP) is a term used in molecular biology and genetics. It refers to the presence of variations in DNA sequences among individuals, which can be detected by restriction enzymes. These enzymes cut DNA at specific sites, creating fragments of different lengths.

In RFLP analysis, DNA is isolated from an individual and treated with a specific restriction enzyme that cuts the DNA at particular recognition sites. The resulting fragments are then separated by size using gel electrophoresis, creating a pattern unique to that individual's DNA. If there are variations in the DNA sequence between individuals, the restriction enzyme may cut the DNA at different sites, leading to differences in the length of the fragments and thus, a different pattern on the gel.

These variations can be used for various purposes, such as identifying individuals, diagnosing genetic diseases, or studying evolutionary relationships between species. However, RFLP analysis has largely been replaced by more modern techniques like polymerase chain reaction (PCR)-based methods and DNA sequencing, which offer higher resolution and throughput.

Inosine Monophosphate Dehydrogenase (IMDH or IMPDH) is an enzyme that is involved in the de novo biosynthesis of guanine nucleotides. It catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), which is the rate-limiting step in the synthesis of guanosine triphosphate (GTP).

There are two isoforms of IMPDH, type I and type II, which are encoded by separate genes. Type I IMPDH is expressed in most tissues, while type II IMPDH is primarily expressed in lymphocytes and other cells involved in the immune response. Inhibitors of IMPDH have been developed as immunosuppressive drugs to prevent rejection of transplanted organs. Defects in the gene encoding IMPDH type II have been associated with retinal degeneration and hearing loss.

Water pollution is defined medically as the contamination of water sources by harmful or sufficient amounts of foreign substances (pathogens, chemicals, toxic compounds, etc.) which tend to interfere with its normal functioning and can have negative effects on human health. Such pollutants can find their way into water bodies through various means including industrial waste disposal, agricultural runoff, oil spills, sewage and wastewater discharges, and accidental chemical releases, among others.

Exposure to polluted water can lead to a range of health issues, from minor problems like skin irritation or stomach upset, to severe conditions such as neurological disorders, reproductive issues, cancer, and even death in extreme cases. It also poses significant risks to aquatic life, disrupting ecosystems and leading to the decline or extinction of various species. Therefore, maintaining clean and safe water supplies is critical for both human health and environmental preservation.

I'm sorry for any confusion, but "Fresh Water" is not a medical term. It is a term used to describe water that contains low concentrations of dissolved salts and other dissolved minerals. It is distinguished from saline water, which includes saltwater found in the ocean and brackish water found in estuaries. Fresh water is essential for many biological processes and is the primary source of water for human consumption, agriculture, and industrial use.

Isospora is a genus of protozoan parasites that belong to the phylum Apicomplexa. These parasites are the causative agents of coccidiosis, a type of gastrointestinal infection that primarily affects birds and mammals, including humans. The disease is characterized by watery diarrhea, abdominal pain, vomiting, and weight loss.

Isospora species have a complex life cycle that involves two hosts: an intermediate host, where the parasite reproduces asexually, and a definitive host, where the parasite undergoes sexual reproduction. The infectious stage of the parasite is called an oocyst, which is shed in the feces of the infected host and can survive in the environment for long periods. When ingested by another host, the oocyst releases sporozoites, which invade the intestinal cells and multiply, causing damage to the intestinal lining and leading to the symptoms of coccidiosis.

In humans, Isospora belli is the most common species that causes infection. It is typically transmitted through the fecal-oral route, either by ingesting contaminated food or water or by person-to-person contact. Immunocompromised individuals, such as those with HIV/AIDS, are at higher risk of developing severe and chronic infections with Isospora. Treatment usually involves the use of antiprotozoal drugs, such as trimethoprim-sulfamethoxazole.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

Food parasitology is not a commonly used term in medical or scientific communities. However, it generally refers to the study of parasites that are transmitted through food, including parasitic protozoa, helminths (worms), and arthropods (e.g., tapeworms, roundworms, Giardia, Cryptosporidium, etc.). Food parasitology involves understanding the life cycles, epidemiology, diagnosis, treatment, and prevention of these foodborne parasites. It is an important field within medical and veterinary parasitology, as well as food safety and public health.

Sewage is not typically considered a medical term, but it does have relevance to public health and medicine. Sewage is the wastewater that is produced by households and industries, which contains a variety of contaminants including human waste, chemicals, and other pollutants. It can contain various pathogens such as bacteria, viruses, and parasites, which can cause diseases in humans if they come into contact with it or consume contaminated food or water. Therefore, the proper treatment and disposal of sewage is essential to prevent the spread of infectious diseases and protect public health.

Zoonoses are infectious diseases that can be transmitted from animals to humans. They are caused by pathogens such as viruses, bacteria, parasites, or fungi that naturally infect non-human animals and can sometimes infect and cause disease in humans through various transmission routes like direct contact with infected animals, consumption of contaminated food or water, or vectors like insects. Some well-known zoonotic diseases include rabies, Lyme disease, salmonellosis, and COVID-19 (which is believed to have originated from bats). Public health officials work to prevent and control zoonoses through various measures such as surveillance, education, vaccination, and management of animal populations.

Chlorine compounds refer to chemical substances that contain chlorine (Cl), which is a member of the halogen group in the periodic table. Chlorine is a highly reactive element that readily forms compounds with many other elements and molecules.

Chlorine compounds can be found in various forms, including inorganic and organic compounds. Inorganic chlorine compounds include salts of hydrochloric acid, such as sodium chloride (table salt), and chlorides of metals, such as copper chloride and silver chloride. Other inorganic chlorine compounds include chlorine gas (Cl2), hypochlorous acid (HClO), and chlorine dioxide (ClO2).

Organic chlorine compounds are those that contain carbon atoms bonded to chlorine atoms. Examples of organic chlorine compounds include chlorinated solvents, such as trichloroethylene and perchloroethylene, and pesticides, such as DDT and lindane.

Chlorine compounds have a wide range of uses in various industries, including water treatment, disinfection, pharmaceuticals, agrochemicals, and manufacturing. However, some chlorine compounds can be harmful or toxic to humans and the environment, particularly if they are released into the air, water, or soil in large quantities. Therefore, it is essential to handle and dispose of chlorine compounds properly to minimize potential health and environmental risks.

Genotype, in genetics, refers to the complete heritable genetic makeup of an individual organism, including all of its genes. It is the set of instructions contained in an organism's DNA for the development and function of that organism. The genotype is the basis for an individual's inherited traits, and it can be contrasted with an individual's phenotype, which refers to the observable physical or biochemical characteristics of an organism that result from the expression of its genes in combination with environmental influences.

It is important to note that an individual's genotype is not necessarily identical to their genetic sequence. Some genes have multiple forms called alleles, and an individual may inherit different alleles for a given gene from each parent. The combination of alleles that an individual inherits for a particular gene is known as their genotype for that gene.

Understanding an individual's genotype can provide important information about their susceptibility to certain diseases, their response to drugs and other treatments, and their risk of passing on inherited genetic disorders to their offspring.

Cryptosporidium parvum is one of several species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal ... Cryptosporidium parvum is considered to be the most important waterborne pathogen in developed countries. The protozoa also ... Cryptosporidium parvum glycoproteins have the characteristics of attractive vaccine candidates. Many are immunodominant, and ... Deng, M.; Lancto, C. A.; Abrahamsen, M. S. (2004). "Cryptosporidium parvum regulation of human epithelial cell gene expression ...
3HKO: Crystal structure of a cdpk kinase domain from cryptosporidium Parvum, cgd7_40. ...
Structure of Triosephosphate Isomerase from Cryptosporidium Parvum at 1.55A Resolution ... Cryptosporidium parvum is one of several Cryptosporidium spp. that cause the parasitic infection cryptosporidiosis. ... Cryptosporidium parvum Iowa II. Mutation(s): 0 Gene Names: cgd1_3040. EC: 5.3.1.1. ... Structure of triosephosphate isomerase from Cryptosporidium parvum.. Nguyen, T.N., Abendroth, J., Leibly, D.J., Le, K.P., Guo, ...
Structure of triosephosphate isomerase from Cryptosporidium parvum ...
Correlation between Subtypes of Cryptosporidium parvum in Humans and Risk Paul R. Hunter*, Stephen J. Hadfield†, Dawn Wilkinson ... Correlation between Subtypes of Cryptosporidium parvum in Humans and Risk. ...
2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD. ... Cryptosporidium parvum Iowa II. Mutation(s): 0 Gene Names: cgd3_80. EC: 3.3.1.1. ... 2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.. * ... 2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.. ...
Faecal specimens from calves, 3-13 days old, were screened for oocysts of C. parvum using Ziehl-Neelsen staining and both for ... However, in 35 calves assessed for mixed infections of C. parvum and Giardia sp., oocysts of Cryptosporidium were found in 18 ( ... using direct immunofluorescent (MerIFluor Cryptosporidium/Giardia) assay. The oocysts of C. parvum assessed by Ziehl-Neelsen ... Faecal specimens from calves, 3-13 days old, were screened for oocysts of C. parvum using Ziehl-Neelsen staining and both for ...
Cryptosporidium parvum is an infectious enteric protozoan parasite that causes waterborne disease, severe gastroenteritis and ... Unique cultural methods used to detect viable cryptosporidium parvum oocysts in environmental samples T. R. Slifko; T. R. ... Cryptosporidium parvum is an infectious enteric protozoan parasite that causes waterborne disease, severe gastroenteritis and ... This project has focused on low level detection of Cryptosporidium parvum in environmental samples using a unique cultural ...
Infection by Cryptosporidium parvum triggers a complex array of innate and adaptive cell mediated immune response, playing an ... Infection by Cryptosporidium parvum triggers a complex array of innate and adaptive cell mediated immune response, playing an ... Dynamics of cytokines and immunoglobulins serum profiles in primary and secondary Cryptosporidium parvum infection: Usefulness ... Dynamics of cytokines and immunoglobulins serum profiles in primary and secondary Cryptosporidium parvum infection: Usefulness ...
Parasit Vectors 14:238, 2021). The authors found that Cryptosporidium infection induced significantly aberrant expression of ... Wang, Y., Zhao, H., Zhang, Y. et al. Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum. ... Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB ... Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum. *Yuqing Wang1, ...
This study investigated Cryptosporidium parvum oocyst deposition onto biofilms as a function of shear stress under laminar or ... This study investigated Cryptosporidium parvum oocyst deposition onto biofilms as a function of shear stress under laminar or ... Role of wall shear stress on Cryptosporidium parvum oocyst attachment to environmental biofilms.. Xia Luo, Kristen L Jellison, ... C. parvum deposition onto biofilms followed a pseudo-second-order model under both laminar (after a lag phase) and turbulent ...
Malic, citric, and tartaric acids at concentrations from 1%-5% inhibited C. parvum infectivity of HCT-8 cells by up to ... In this study, alternative treatments to pasteurization were evaluated for their ability to inhibit C. parvum oocyst ... parvum viability to > 90% as monitored in the cell culture assay. It is hypothesized that oocyst wall proteins that are ... parvum infectivity as determined with an MPN-based cell culture infectivity assay. Treating apple cider, orange juice, and ...
... observations of vegetative forms of Acanthamoeba castellanii co-incubated with Cryptosporidium parvum. Red arrows show ... Detection of Cryptosporidium oocysts in green mussels (Perna viridis) from shell-fish markets of Thailand Parasite, 2009, 16, ... Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium ...
Cryptosporidium parvum. Cyclospora cayetanensis. Escherichia coli O157:H7 and related E. coli (e.g., O111:NM and O104:H21). ...
... ... Introduction: Infections with the apicomplexan obligate intracellular parasite Cryptosporidium parvum lead to cryptosporidiosis ... parvum-sporozoites and oocysts, thus as a stage-independent cell death process. Our data further showed that C. parvum strongly ... C. parvum is one of the most common diarrheal pathogens in young calves, which are the main reservoir of the pathogen. ...
Gong, A. Y., Zhou, R., Hu, G., Liu, J., Sosnowska, D., Drescher, K. M., Dong, H., & Chen, X. M. (2010). Cryptosporidium parvum ... Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. / Gong, Ai Yu; Zhou, Rui; Hu ... Gong, AY, Zhou, R, Hu, G, Liu, J, Sosnowska, D, Drescher, KM, Dong, H & Chen, XM 2010, Cryptosporidium parvum induces B7-H1 ... Cryptosporidium parvum induces B7-H1 expression in cholangiocytes by down-regulating microRNA-513. In: Journal of Infectious ...
Sooleviiruste (adeno-, rota-, astro-, noroviiruse) ja sooleparasiitide (Giardia lamblia, Cryptosporidium parvum) antigeenide ... Sooleviiruste (adeno-, rota-, astro-, noroviiruse) ja sooleparasiitide (Giardia lamblia, Cryptosporidium parvum) antigeenide ... Cryptosporidium parvum) antigeenide määramise immunokromatograafilisel meetodil.. Seni kasutusel olnud viiruste ja parasiitide ... Cryptosporidium spp* DNA. * Sisaldab liike C. andersoni, C. canis, C. chipmunk genotüüp 1, C. cuniculus, C. felis, C. hominis, ...
Cryptosporidium parvum: Administer paromomycin; however, effectiveness is not proven. Nitazoxanide, a newer anthelmintic, is ... Antimotility agents are not indicated for infectious diarrhea, except for refractory cases of Cryptosporidium infection. ...
Cryptosporidium parvum in children with diarrhea in Mulago Hospital, Kampala, Uganda.. Title. Cryptosporidium parvum in ... Cryptosporidium parvum was detected and genotyped among 2,446 children of whom 1,779 (72.7%) had diarrhea, and 667 (27.3%) were ... Animals, Case-Control Studies, Child, Preschool, Cross-Sectional Studies, Cryptosporidiosis, Cryptosporidium parvum, Diarrhea, ... parvum, 24 (12.6%) died, compared with 34 (6.2%) of the 545 without C. parvum (P = 0.005). Mortality rates were higher among ...
Cryptosporidium parvum is an enteric protozoan, which causes severe diarrhoea in a wide range of vertebrate hosts including ... Clinical cases of zoonotic Cryptosporidium parvum (subtype IIdA15G1) infections in Korean goats. ... C. parvum infections are responsible for immense economic losses to the livestock industry; furthermore, the zoonotic spread of ...
Lab 17 Cryptosporidium Parvum Surface Antigens. [PDF - 121 KB] Lab 17 Toxoplasma confirmation tests. [PDF - 20 KB] ...
Water safety threats (e.g., Vibrio cholerae, Cryptosporidium parvum). Y. *Yersinia pestis (plague) ...
Cryptosporidium parvum oocysts were detected in feces of dairy calves raised in Rio de Janeiro State and the risk factors ... Detectaram-se oocistos de Cryptosporidium parvum em fezes de bezerros leiteiros no estado do Rio de Janeiro e analisaram-se os ... Animais; Bovinos/classificação; Cryptosporidium parvum/patogenicidade; Fatores de Risco; Eucariotos; Infecções/microbiologia; ... Risk factors associated with the occurrence of Cryptosporidium parvum infection in calves / Fatores de risco associados à ...
The occurrence of Cryptosporidium parvum and C. muris in wild rodents and insectivores in Spain.. Veterinary Parasitology. 92, ... Occurrence of Cryptosporidium oocysts and Giardia cysts in water from irrigation channels in Catalonia (NE Spain). Revista ... Transmission dynamics of Cryptosporidium in primates and herbivores at the Barcelona Zoo: a long-term study. Veterinary ... Faecal contamination in public pools in Barcelona province: Cryptosporidium spp. and bacterial indicators. Journal Of Water And ...
"The unusual mitochondrial compartment of Cryptosporidium parvum". Trends in Parasitology. 21 (2): 68-74. doi:10.1016/j.pt. ... In these cases, genes encoded by the mitochondrial DNA have been lost or transferred to the nucleus.[128] Cryptosporidium have ... mitochondria that lack any DNA, presumably because all their genes have been lost or transferred.[177] In Cryptosporidium, the ...

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