Cell extracts. Medical search

Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)

Xeroderma pigmentosum variant (XPV) cells are characterized by a cellular defect in the ability to synthesize intact daughter DNA strands on damaged templates. Molecular mechanisms that facilitate replication fork progression on damaged DNA in normal cells are not well defined. In this study, we used single-stranded plasmid molecules containing a single N-2-acetylaminofluorene (AAF) adduct to analyze translesion synthesis (TLS) catalyzed by extracts of either normal or XPV primary skin fibroblasts. In one of the substrates, the single AAF adduct was located at the 3' end of a run of three guanines that was previously shown to induce deletion of one G by a slippage mechanism. Primer extension reactions performed by normal cellular extracts from four different individuals produced the same distinct pattern of TLS, with over 80% of the products resulting from the elongation of a slipped intermediate and the remaining 20% resulting from a nonslipped intermediate. In contrast, with cellular extracts from five different XPV patients, the TLS reaction was strongly reduced, yielding only low amounts of TLS via the nonslipped intermediate. With our second substrate, in which the AAF adduct was located at the first G in the run, thus preventing slippage from occurring, we confirmed that normal extracts were able to perform TLS 10-fold more efficiently than XPV extracts. These data demonstrate unequivocally that the defect in XPV cells resides in translesion synthesis independently of the slippage process. (+info)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)

Oncoprotein 18/stathmin (Op18) is a recently identified phosphorylation-responsive regulator of the microtubule (MT) system. It was originally proposed that Op18 specifically regulates dynamic properties of MTs by associating with tubulin, but it has subsequently been proposed that Op18 acts simply by sequestering of tubulin heterodimers. We have dissected the mechanistic action of Op18 by generation of two distinct classes of mutants. One class has interruptions of the heptad repeats of a potential coiled-coil region of Op18, and the other involves substitution at all four phosphorylation sites with negatively charged Glu residues. Both types of mutation result in Op18 proteins with a limited decrease in tubulin complex formation. However, the MT-destabilizing activities of the coiled-coil mutants are more severely reduced in transfected leukemia cells than those of the Glu-substituted Op18 derivative, providing evidence for tubulin-directed regulatory activities distinct from tubulin complex formation. Analysis of Op18-mediated regulation of tubulin GTPase activity and taxol-promoted tubulin polymerization showed that while wild-type and Glu-substituted Op18 derivatives are active, the coiled-coil mutants are essentially inactive. This suggests that Op18-tubulin contact involves structural motifs that deliver a signal of regulatory importance to the MT system. (+info)

Regulation of human hsp90alpha gene expression. (3/1728)

Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different. (+info)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)

Initiation of translation of the animal picornavirus RNAs occurs via a mechanism of direct ribosome entry, which requires a segment of the 5' UTR of the RNA, known as the internal ribosome entry site (IRES). In addition, translation of the enterovirus and rhinovirus (HRV) subgroups requires cellular trans-acting factors that are absent from, or limiting in rabbit reticulocytes, but are more abundant in HeLa cell extracts. It has been shown previously that HeLa cells contain two separable activities, each of which independently stimulates HRV IRES-dependent translation when used to supplement reticulocyte lysate; one of these activities was identified as polypyrimidine tract-binding protein (PTB). Here, the purification of the second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR. It comprises two components: a 38-kD protein (p38), which is a novel member of the GH-WD repeat protein family and has no intrinsic RNA-binding activity; and a 96- to 97-kD protein doublet, which was identified as unr, an RNA-binding protein with five cold-shock domains. Coimmunoprecipitation with antibodies against either protein shows that the two proteins interact with each other, and thus p38 is named unrip (unr-interacting protein). Recombinant unr acts synergistically with recombinant PTB to stimulate translation dependent on the rhinovirus IRES. In contrast, unr did not significantly augment the PTB-dependent stimulation of poliovirus IRES activity. (+info)

Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)

Round spermatids, precursor male gametes, are known to possess the potential to achieve fertilization and embryonic development when injected into eggs. However, injection of spermatids alone seldom activates eggs in the mouse, as spermatids by themselves cannot induce an increase in intracellular Ca2+, a prerequisite for egg activation. We injected a mouse round spermatid into an egg simultaneously with partially purified sperm factor from differentiated hamster spermatozoa. The combined injection produced repetitive Ca2+ increases (Ca2+ oscillations) lasting for at least 4 h as observed at fertilization, and induced activation in 92% of eggs. This method provided 75% fertilization success associated with male and female pronucleus formation and development to 2-cell embryos, while only 7% of eggs were fertilized by injection of a spermatid alone. Of the 2-cell embryos, approximately 50% developed to blastocysts during 5 days of culture in vitro, while no blastocysts were obtained following injection of sperm factor alone. Furthermore, the 2-cell embryos, that were created by spermatids and sperm factor and transplanted into foster mothers, developed into normal offspring, although the percentage was only 22%. All infants grew into healthy adults carrying normal chromosomes. The sperm factor served as a complementary factor for successful fertilization by round spermatid injection. (+info)

Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)

The Ca channels of male germ-line cells are partially characterized, but the molecular properties and subcellular localization of the Ca channels of mature sperm are unknown. Here, we probe rodent sperm with anti-peptide antibodies directed to cytosolic domains of cloned rat brain alpha1A, alpha1C, and alpha1E Ca channel subunits. Each recognizes a 200- to 245-kDa band on immunoblots of whole rat sperm extracts. A smaller ( approximately 110-kDa) alpha1C band also is detected. Confocal fluorescence images of mouse sperm show characteristic patterns of punctate alpha1A-, alpha1C-, and alpha1E-immunoreactivity. For alpha1A, the puncta are larger, less numerous, and more variable in distribution than for alpha1C and alpha1E. They are absent from the acrosomal crescent, but are present elsewhere over the sperm head, often at the apical tip and equatorial segment. They also are found at irregular intervals along both the midpiece and the principal piece of the flagellum. For alpha1C and alpha1E, puncta are dense along dorsal and ventral aspects of the acrosomal cap. For alpha1E but not alpha1C, the remainder of the acrosomal region also is labeled. Neither is found in the postacrosomal region or on the midpiece. Puncta of alpha1C and alpha1E occur at regular intervals each in two parallel rows, at the dorsal and ventral aspects of the proximal segment of the flagellar principal piece. The puncta in these arrays become less abundant and intense in the distal flagellum. These results demonstrate that multiple Ca channel proteins are present in mature sperm and are regionally localized in ways that may give them different regulatory roles. (+info)

Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)

Metastatic cancer in adults usually has a fatal outcome. In contrast, advanced testicular germ cell tumours are cured in over 80% of patients using cisplatin-based combination chemotherapy [1]. An understanding of why these cells are sensitive to chemotherapeutic drugs is likely to have implications for the treatment of other types of cancer. Earlier measurements indicate that testis tumour cells are hypersensitive to cisplatin and have a low capacity to remove cisplatin-induced DNA damage from the genome [2] [3]. We have investigated the nucleotide excision repair (NER) capacity of extracts from the well-defined 833K and GCT27 human testis tumour cell lines. Both had a reduced ability to carry out the incision steps of NER in comparison with extracts from known repair-proficient cells. Immunoblotting revealed that the testis tumour cells had normal amounts of most NER proteins, but low levels of the xeroderma pigmentosum group A protein (XPA) and the ERCC1-XPF endonuclease complex. Addition of XPA specifically conferred full NER capacity on the testis tumour extracts. These results show that a low XPA level in the testis tumour cell lines is sufficient to explain their poor ability to remove cisplatin adducts from DNA and might be a major reason for the high cisplatin sensitivity of testis tumours. Targeted inhibition of XPA could sensitise other types of cells and tumours to cisplatin and broaden the usefulness of this chemotherapeutic agent. (+info)

Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)

Apoptosis is accompanied by the activation of a number of apoptotic proteases (caspases) which selectively cleave specific cellular substrates. Caspases themselves are zymogens which are activated by proteolysis. It is widely believed that 'initiator' caspases are recruited to and activated within apoptotic signalling complexes, and then cleave and activate downstream 'effector' caspases. While activation of the effector caspase, caspase-3, has indeed been observed as distal to activation of several different initiator caspases, evidence for a further downstream proteolytic cascade is limited. In particular, there is little evidence that cellular levels of caspase-3 that are activated via one pathway are sufficient to cleave and activate other initiator caspases. To address this issue, the ability of caspase-3, activated upon addition to cytosolic extracts of cytochrome c, to cause cleavage of caspase-2 was investigated. It was demonstrated that cleavage of caspase-2 follows, and is dependent upon, activation of caspase-3. Moreover, the activation of both caspases was inhibited by Bcl-2. Together, these data indicate that Bcl-2 can protect cells from apoptosis by acting at a point downstream from release of mitochondrial cytochrome c, thereby preventing a caspase-3 dependent proteolytic cascade. (+info)

Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or ... Doing so may allow specific compounds to be extracted or to produce an extract without the hydrolysis of cell contents (as in ... switched from beef extract to yeast extract for 2005 and most of 2006, but later switched back. Yeast extract is used as a ... Yeast extracts in general are produced in three steps: fermentation (growing the yeast), disruption (breaking of the cells), ...

https://en.wikipedia.org/wiki/Yeast_extract

"Interaction of astringent grape seed procyanidins with oral epithelial cells". Food Chemistry. 115 (2): 551-557. doi:10.1016/j. ... Grape seed extract is an industrial derivative of whole grape seeds. The extract contains proanthocyanidins. Grape seed extract ... Grape seed extract is sold as a dietary supplement, but there is insufficient medical evidence it provides any effect on human ... Zhang H, Liu S, Li L, Liu S, Liu S, Mi J, Tian G (2016). "The impact of grape seed extract treatment on blood pressure changes ...

https://en.wikipedia.org/wiki/Grape_seed_extract

v t e (CS1 maint: archived copy as title, Cell culture media, All stub articles, Microbiology stubs). ... Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, ... This culture is to be differentiated from regular yeast extract agar, which is not selective. "Archived copy" (PDF). Archived ... "Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar". Am. J. Ophthalmol. 126 (4): 590-2. ...

https://en.wikipedia.org/wiki/Buffered_charcoal_yeast_extract_agar

The main chapters imported in 2012 were: coffee (75.3% of the total); juices and vegetable extracts (18.9%); nuts (3.5%); ... The main exported chapters consisted of: sounding devices (44.5% of the total); photovoltaic cells (20.4%); centrifugal pumps ( ...

https://en.wikipedia.org/wiki/Rwanda–Spain_relations

Ning Y, Rao Y, Yu Z, Liang W, Li F (March 2016). "Skin permeation profile and anti-inflammatory effect of anemonin extracted ... and effects on Caco-2 cells by in vitro and in silico studies". Open Chemistry. 20 (1): 299-312. doi:10.1515/chem-2022-0137. ... Pirvu, Lucia; Stefaniu, Amalia; Neagu, Georgeta; Pintilie, Lucia (2022-01-01). "Studies on Anemone nemorosa L. extracts; ... ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells". Journal of Ethnopharmacology. 104 (3): 362-366. ...

https://en.wikipedia.org/wiki/Anemonin

The structure and properties of levan, a polymer of d-fructose produced by cultures and cell-free extracts of Aerobacter ... The structure and properties of levan, a polymer of d-fructose produced by cultures and cell-free extracts of Aerobacter ... 78:401-406, 1958 Xylosyl transfer by asparagus extracts. Feingold, D. S., Neufeld, E. F. and Hassid, W. Z. J. Biol. Chem. 234: ... Interferon production in mice by cell wall mutants of Salmonella typhimurium. Youngner, J. S. and Feingold, D. S. Virology 1: ...

https://en.wikipedia.org/wiki/David_Sidney_Feingold

Cell. 122 (3): 379-91. doi:10.1016/j.cell.2005.06.015. PMC 4401993. PMID 16096058. Glanzer J, Miyashiro KY, Sul JY, Barrett L, ... Jamison SF, Crow A, Garcia-Blanco MA (October 1992). "The spliceosome assembly pathway in mammalian extracts". Molecular and ... doi:10.1016/j.cell.2017.04.033. PMID 28502770. Schmucker D, Clemens JC, Shu H, Worby CA, Xiao J, Muda M, Dixon JE, Zipursky SL ... Zhang X, Yan C, Hang J, Finci LI, Lei J, Shi Y (May 2017). "An Atomic Structure of the Human Spliceosome". Cell. 169 (5): 918- ...

https://en.wikipedia.org/wiki/Spliceosome

The company extracts cells from live animals. It cultures those cells in stainless steel fermenters, where they reproduce and ... Purdy, Chase (22 January 2020). "A startup says it's building a US pilot plant for cell-based meat". Quartz. Retrieved 27 May ... Future Meat Technologies, or Future Meat for short, is a biotechnology firm which produces cultured meat from chicken cells and ... Future Meat Technologies mainly seeks to supply hardware and cell lines to manufacturers of cultured meat rather than directly ...

https://en.wikipedia.org/wiki/Future_Meat_Technologies

A cell extract is obtained by lysing the cell of interest and centrifuging out the cell walls, DNA genome, and other debris. ... Common cell extracts in use today are made from E. coli (ECE), rabbit reticulocytes (RRL), wheat germ (WGE), insect cells (ICE ... Common components of a cell-free reaction include a cell extract, an energy source, a supply of amino acids, cofactors such as ... Most notably, a cell-free reaction, including extract preparation, usually takes 1 -2 days, whereas in vivo protein expression ...

https://en.wikipedia.org/wiki/Cell-free_protein_synthesis

Kornberg T, Gefter ML (1971). "Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II". Proc. ... Kornberg T, Gefter ML (10 September 1972). "Deoxyribonucleic acid synthesis in cell-free extracts. IV. Purification and ...

https://en.wikipedia.org/wiki/Thomas_B._Kornberg

1 Preparation and properties of cell-free extracts". J. Biol. Chem. 240 (12): 4669-74. doi:10.1016/S0021-9258(18)97007-9. PMID ...

https://en.wikipedia.org/wiki/Ethanolamine_ammonia-lyase

If applied to the skin, the extract sanguinarine may cause a massive scab of dead flesh where it killed the cells, called an ... Bloodroot extracts have also been promoted by some dietary supplement companies as a treatment or cure for cancer, but the U.S ... Sanguinarine kills animal cells by blocking the action of Na+/K+-ATPase transmembrane proteins. As a result, applying bloodroot ... Bloodroot and its extracts are thus considered escharotic. Although applying escharotic agents (including bloodroot) to the ...

https://en.wikipedia.org/wiki/Sanguinaria

"Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium". Arch. Biochem. Biophys. 242 (2): 430-9. ...

https://en.wikipedia.org/wiki/Methenyltetrahydromethanopterin_cyclohydrolase

... extract against two types of herpes simplex viruses in cell culture. Die Pharmazie-An International Journal of Pharmaceutical ... Extracting seed from the fruit involves cleaning the flesh off by placing fruits in a macerator, blender, or by soaking the ... Rayne, S., & Mazza, G. (2007). Biological activities of extracts from sumac (Rhus spp.): a review. Nature Precedings, 1-1. ... ovata extract have been proven to have antibacterial properties that impede the growth of gram-positive bacteria like ...

https://en.wikipedia.org/wiki/Rhus_ovata

Philpott A, Leno GH (May 1992). "Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts". Cell. 69 (5): 759-767. doi: ... Seminars in Cell Biology. 1 (1): 11-17. PMID 1983266. Rice P, Garduño R, Itoh T, Katagiri C, Ausio J (June 1995). " ... Cell. 30 (2): 449-458. doi:10.1016/0092-8674(82)90242-2. PMID 6814762. Tejun S, Yaozhou Z (2007). "Nucleoplasmin, an Important ...

https://en.wikipedia.org/wiki/Nucleoplasmin

Cell. Biochem. 5 (1-2): 55-63. doi:10.1007/BF01874172. PMID 4279328. S2CID 26999163. Kalckar, H. M. Biological phosphorylations ... Kalckar, H. M. (1939). "The Nature of Phosphoric Esters Formed in Kidney Extracts". Biochemical Journal. 33 (5): 631-641. doi: ... which they purified from muscle extracts. Further work on nucleotide metabolism allowed him to identify nucleoside ...

https://en.wikipedia.org/wiki/Herman_Kalckar

The model has three basic predictions that should hold true in cycling oocyte extracts whose cell cycle progression is ... After the cell has split into its two daughter cells, the cell enters G1. DNA repair processes and cell cycle checkpoints have ... During this process, known as the cell cycle, a cell duplicates its contents and then divides in two. The purpose of the cell ... and the cell wall forms a septum which divides the cell into two. As the eukaryotic cell cycle is a complex process, eukaryotes ...

https://en.wikipedia.org/wiki/Cell_cycle_checkpoint

Hydrolysis of nucleic acids in crude cell-free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies ... A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal ...

https://en.wikipedia.org/wiki/Micrococcal_nuclease

Studies have shown that its extracts have renal effects. It has also been seen that in rats, such extracts cause an increase in ... The surface cells are loosely arranged. The number of chromosomes is 16, which is 2n = 8. It has been said that the species of ... "Effects of hydroalcoholic extracts of Portulaca pilosa and Achyrocline satureioides on urinary sodium and potassium excretion ...

https://en.wikipedia.org/wiki/Portulaca_pilosa

"Toxic effects of Karenia mikimotoi extracts on mammalian cells". Chinese Journal of Oceanology and Limnology. 29 (4): 860-868. ...

https://en.wikipedia.org/wiki/Karenia_mikimotoi

The antigens are either from cell extracts or recombinant. Blood serum is incubated in the wells of the plate and is washed out ... T-cells and B-cells) and antigen presenting cells. These cells coordinate an immune response upon the detection of foreign ... the cell line was contaminated and displaced by HeLa cells, and has now been identified as actually HeLa cells. They are ... They are termed ENA because they can be extracted from the cell nucleus with saline. The ENAs consist of ribonucleoproteins and ...

https://en.wikipedia.org/wiki/Antinuclear_antibody

One study showed that extracts of H. stigonocarpa suppressed cell division in onions, and this may imply that it contains some ... Fabaceae) on the cell cycle of Allium cepa L." Anais da Academia Brasileira de Ciências. 86 (3): 1147-1150. doi:10.1590/0001- ... Lacerda, Lourran P.; Malaquias, Geiz; Peron, Ana Paula (2014). "Antiproliferative action of aqueous extracts of Hymenaea ...

https://en.wikipedia.org/wiki/Hymenaea_stigonocarpa

Research in the early 20th century focused on extracting crude extracts and reconciling some of the conflicting views on ... Sugawara, I (1982). "Polysaccharides with sulfate groups are human T-cell mitogens and murine polyclonal B-cell activators ( ... Methods of extracts and isolation of fucoidan from brown seaweeds were determined on laboratory scale by Black et al. at the ... Some extracts are consumed via energy drinks or within vitamin-enriched powdered supplement packets that are mixed into water. ...

https://en.wikipedia.org/wiki/Fucoidan

Knysh O, Martynov A (2020). "Lactobacillus reuteri cell-free extracts against antibiotic-resistant bacteria". Zaporozhye ... Cell. 165 (7): 1762-1775. doi:10.1016/j.cell.2016.06.001. PMC 5102250. PMID 27315483. Wang XD, Soltesz V, Molin G, Andersson R ...

https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri

Fermentation is another process by which cells can extract energy from glucose. It is not a form of cellular respiration, but ... This phenomenon is called the Warburg effect and is found primarily in cancer cells. Muscles cells under great exertion will ... Generally, lactic acid fermentation occurs only when aerobic cells are lacking oxygen. However, some aerobic mammalian cells ... CO2 is excreted from the cell via diffusion into the blood stream, where it is transported in three ways: Up to 7% is dissolved ...

https://en.wikipedia.org/wiki/Cellular_waste_product

... is typically extracted by ethanol precipitation from liquid culture or by alkaline treatment from the cell wall. Once extracted ... Cell Microbiol 12, 405-410 Robinet, P., Baychelier, F., Fontaine, T., Picard, C., Debre, P., Vieillard, V., Latge, J.P., and ... Bardalaye, P.C., and Nordin, J.H. (1976). Galactosaminogalactan from cell walls of Aspergillus niger. J Bacteriol 125, 655-669 ... Similar to other fungal cell wall polysaccharides, galactosaminogalactan is synthesized by polymerization of nucleotide sugars ...

https://en.wikipedia.org/wiki/Galactosaminogalactan

It is extracted from bovine thymus. Prior to 1960, the thymus gland, which lies in the cervical thoracic area, was thought to ... To attack tumor cells and viruses, CD-4 cells coordinate the overall immune response and help activate CD8 T-lymphocytes. Often ... This protein came to be known as lymphocyte T-cell immunomodulator. Lymphocyte T-cell immunomodulator, or T-4 immune ... CD-8+ T-lymphocytes they respond to intracellular pathogens and cancer cells. Under viral attack CD-4+ T-cells fail to mature, ...

https://en.wikipedia.org/wiki/Lymphocyte_T-cell_immunomodulator

Cell. 132 (4): 583-597. doi:10.1016/j.cell.2008.02.007. PMID 18295577. Cohen, A. R. (3 November 2014). "Extracting meaning from ... In cell biology, single-cell variability occurs when individual cells in an otherwise similar population differ in shape, size ... Similar to variation in the metabolome, the proteins present in a cell and their abundances can vary from cell to cell in an ... but they are designed to use DNA from a population of cells rather than a single cell. The primary challenge for single-cell ...

https://en.wikipedia.org/wiki/Single-cell_variability

Through experiments in Xenopus laevis cell-free egg extracts, such model was confirmed as the basis for entry into mitosis. ... Cells with a defective G2-M checkpoint will undergo apoptosis or death after cell division if they enter the M phase before ... September 2002). "Hysteresis drives cell-cycle transitions in Xenopus laevis egg extracts". Proceedings of the National Academy ... The G2-M DNA damage checkpoint is an important cell cycle checkpoint in eukaryotic organisms that ensures that cells don't ...

https://en.wikipedia.org/wiki/G2-M_DNA_damage_checkpoint

Mechanism of action: A monoclonal antibody against HER2/neu (erb-B2). Helps kill breast cancer cells that overexpress HER-2, ... Extracts from some medicinal mushrooms are natural biological response modifiers. Immunotherapy "Biological Response Modifiers ... where targeted therapy often relies on the immune system being used to attack cancer cells) and in treating autoimmune diseases ... "Persistence of Autoreactive IgA-Secreting B Cells Despite Multiple Immunosuppressive Medications Including Rituximab". JAMA ...

https://en.wikipedia.org/wiki/Biological_response_modifier

... exerts a significant effect against breast cancer cell growth and may eventually become a chemopreventive agent against this ... Bitter melon extract decreased breast cancer cell growth. Date:. February 23, 2010. Source:. American Association for Cancer ... "Bitter melon extract decreased breast cancer cell growth." ScienceDaily. www.sciencedaily.com. /. releases. /. 2010. /. 02. /. ... Bitter melon extract, a common dietary supplement, exerts a significant effect against breast cancer cell growth and may ...

https://www.sciencedaily.com/releases/2010/02/100223131956.htm

We provide the first report of the effects of extracts prepared from the shrub ,i,Symphoricarpos occidentalis,/i, in several ... We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of ... Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells Toxins (Basel). 2021 ... S. occidentalis (Caprifoliaceae) extracts are cytotoxic, and, strikingly, treated cells undergo light-dependent vacuolation ...

https://pubmed.ncbi.nlm.nih.gov/33673235/

... Nucleic Acids Res. 1988 Feb 11;16(3):1215. doi: ...

https://pubmed.ncbi.nlm.nih.gov/3344216/?dopt=AbstractPlus

A Japanese mushroom extract was shown to eradicate HPV in cervical cancer cells as well as mouse models of the infection. ... A Japanese mushroom extract was shown to eradicate HPV in cervical cancer cells as well as mouse models of the infection. Photo ... Japanese Mushroom Extract Kills Off HPV In Cervical Cancer Cells. Mar 23, 2014 03:50 PM. By ... Judith A. Smith, a researcher at the University of Texas and lead author of the new study, said that the mushroom extract ...

https://www.medicaldaily.com/japanese-mushroom-extract-kills-hpv-cervical-cancer-cells-272152

The UV-Vis spectra of extracts with and without copper (II) ions were recorded. Viable cell extract and non-viable cell extract ... The cells were separated from the extracts by filtration using paper filters. Cell concentration was determined before ... In order to obtain cells extracts, viable and non-viable cells were suspended in 100mL of double deionized water in 500 mL ... The third approach consisted in using viable and non-viable cells after extract removal to assess whether the cell wall ...

https://www.aiche.org/conferences/aiche-annual-meeting/2015/proceeding/paper/656b-potential-analysis-biosynthesis-copper-nanoparticles-using-fungus-hypocrea-lixii-cell-extract

This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene ... , Find, read and ... Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. ...

https://www.techscience.com/biocell/v47n8/53794/pdf

Treatment with FWGE (1-2%) significantly decreased the level of intracellular ROS compared to LPS-treated cells. ... against LPS-induced inflammation and oxidative stress in IPEC-J2 porcine intestinal epithelial cells was studied. Enterocytes ... the protective effect of a fermented wheat germ extract (FWGE) ... Cell Line and Culture Conditions. The IPEC-J2 cell line was ... S. N. Lee, H. Park, and K. E. Lee, "Cytotoxic activities of fermented wheat germ extract on human gastric carcinoma cells by ...

https://www.hindawi.com/journals/omcl/2020/1482482/

Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of ... By using cell extracts from exonuclease-deficient knockout cells, linear DNA templates remain intact without requiring any end- ... Easier cell-free expression from linear DNA templates in ΔrecB/ΔrecBCD extracts can be deployed for the production of small ... Transfer the cells to chilled and pre-weighed 50 mL tubes.. *Spin down the cells at 2,000 x g for 8 min at 4 °C, and then ...

https://www.jove.com/t/64236/cell-free-protein-synthesis-from-exonuclease-deficient-cellular

Discover the cytotoxic potential of Canarium odontophyllum stem bark extracts against colorectal carcinoma cells. Learn about ... Payne, G.F., Bringi, V., Prince, C. and Shuler, M.L. (1992) Plant Cell and Tissue Culture in Liquid Systems. Hanser Publication ... 1.04 mg/ml compared to methanol extract with IC50 0.122 ± 4.92 mg/ml. No cytotoxic effect was observed by the aqueous extract ... saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. Acetone extract of C. odontophyllum stem bark ...

https://www.scirp.org/journal/paperinformation.aspx?paperid=52569

Based on cell cycle analysis, B. nigra extract significantly arrested A549 and H1299 cells at S and G2/M phases. Additionally, ... nigra seeds against A549 and H1299 human non-small cell lung cancer cell lines. B. nigra extract showed a substantial growth- ... Furthermore, treatment of both A549 and H1299 cells with B. nigra extract alone or in combination with camptothecin induced DNA ... B. nigra extract suppressed the migratory and invasive properties of both cell lines, downregulated the expression of matrix ...

https://www.mdpi.com/1420-3049/25/9/2069

Some cells remained isolated but with altered morphology (Figure 1F). RD, Hep-2C, and BGM cells injected with an extract ... Image of mammalian cell lines injected with extracts from healthy shrimp: A, human rhabdomyosarcoma (RD), Cep-2C, BGM RD cells ... Image of mammalian cell lines injected with extracts from healthy shrimp: A, human rhabdomyosarcoma (RD), Cep-2C, BGM RD cells ... The cells showed an apparent increase in size, diffuse cell rounding, and a refringent aspect (Figure 1B). In Hep-2C cells, the ...

https://wwwnc.cdc.gov/eid/article/9/2/02-0181_article

... extracts are a good source of natural bioactive compounds, including phytosterols. Phytosterols are known to be applied in the ... HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines ... "HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines ... "HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines ...

https://hrcak.srce.hr/301960

... of host cell proteins (HCPs) from tobacco extracts ... ... of host cell proteins (HCPs) from tobacco extracts prior to any ... Tobacco Host Cell Protein Removal Blanching Intact Plants Heat Treatment Of Extracts Chromatographic Purification Recombinant ... Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts. ... Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts ...

https://www.jove.com/v/54343/comparison-tobacco-host-cell-protein-removal-methods-blanching-intact

Michigan State Police Extracting Your Cell Phone Data During Traffic Stops. By Uncle Dave Monday April 18, 2011 ... What are they going to do when the phones are built into the cars? I wonder if these cell phones can be hacked, physically or ... I can almost guarantee the cell phone data will be abused. Since when are the cops sworn not to exploit anything they learn ...

https://www.dvorak.org/blog/2011/04/18/michigan-state-police-extracting-your-cell-phone-data-during-traffic-stops/

... green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis ... green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis ... green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis ... Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce ...

http://ejbiotechnology.info/index.php/ejbiotechnology/article/view/2019.02.001

How can I extract a particular attribute value from tree structure ??? I have refered to the below thread, but it describes to ... One thing that is a bit tricky with trees is that you can have many time the same cell value (displayed string) but each "line ... to explain in single sentence , I am trying to extract one attribute from the attributew tree in this example Grab and ... I havent seen anything related for extracting a particular value from tree control. ...

https://forums.ni.com/t5/LabVIEW/Extracting-a-particular-cell-value-from-Tree-in-Front-pannel/m-p/2344710/highlight/true

Lets say the text is in cell A1. Enter the following formula in another cell: ... Need help in extracting the latest date from a cell by Ansari3108 on September 08, 2022 ... Need help in extracting the latest date from the above string in a cell. ...

https://techcommunity.microsoft.com/t5/excel/need-help-in-extracting-the-latest-date-from-a-cell/m-p/3620391

Extract part of date from cell with Kutools for Excel Extract part of date from cell with formula. To extract year, month, day ... How to quickly extract part of date from cell in Excel?. This article is talking about extracting part of date from a cell in ... and enter it into a blank cell and press Enter key to get extracted part, drag auto fill handle over the cells needed to apply ... Extract part of date from cell with Kutools for Excel. With Kutools for Excels Apply Date Formatting can help you quickly ...

https://www.extendoffice.com/documents/excel/3649-excel-extract-part-of-date.html

... found that leukemia cancer cells exposed to grapeseed extract (GSE) were rapidly killed through a process of cell suicide known ... Study: Grape Seed Extract Kills 76% Of Leukemia Cancer Cells In 24 Hours. January 28, 2012. January 7, 2009. by Infinite ... Protecting healthy cells. Another important finding in the University of Kentucky study is that the grapeseed extract was not ... Grapeseed extract appears to accelerate this process in cancer cells, helping them more rapidly assess their own flawed state ...

https://infiniteunknown.net/2009/01/07/study-grape-seed-extract-kills-76-of-leukemia-cancer-cells-in-24-hours/

Methanol extract of Solanum nigrum Induces Autophagy and Apoptosis in MDA-MB-468 Breast Cancer Cells, A. Shirkavad, Zahra N. ... extract of SN to induce programmed cell death in MDAMB- 468, a TNBC cell line was studied. Mechanism of cell death was ... These findings indicated that Solanum nigrum extract induced cell death in MDA-MB-468 cells by two distinct mechanisms, ... They concluded that cells treated with low concentration of SNE (1.5 mg/ml) result in autophagic cell death and cells treated ...

https://www.ijpsonline.com/articles/methanol-extract-of-isolanum-nigrumi-induces-autophagy-and-apoptosis-in-mdamb468-breast-cancer-cells-3649.html

This study aimed to investigate the effects of green tea extract on A549 cells, a type of non-small lung cancer cells. A549 ... In summary, our results demonstrate that A549 cells are insensitive to both low and high concentrations of green tea extract, ... Cell sensitivity was evaluated using the MTT assay, while cell death was assessed using the Tali image-based cytometer. Ultra ... as blocking autophagy with bafilomycin A1 reduced cell viability and enhanced necrotic cell death in response to GTE treatment ...

https://www.bioinformation.net/019/97320630019860.htm

extract (JIB extract) for melanoma cells. Our results indicated the anti-melanoma activity of JIB extract. JIB extract induced ... JIB extract synergized with cisplatin to block cell cycle progression at the G0/G1 phase in B16/F10 cells. B16/F10 cells (1 × ... JIB extract synergized with cisplatin to repress cell proliferation and survival pathways in B16/F10 cells. B16/F10 cells were ... JIB extract synergized with cisplatin to contribute to cell apoptosis in B16/F10 cells. B16/F10 cells (1 × 106/dish) were ...

https://www.medsci.org/v18p0157.htm

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How I can extract the name of the sheet and pasted it in a cell, with a function? (not using Macros and VBA) Some one can help ... For example, if I want to extract the name of the last sheet that I have in a book, and put it into a cell in the first sheet ... Hi, I would like to know, How I can extract the name of the sheet and pasted it in a cell, with a function? (not using Macros ... How I can extract the name of the sheet and pasted it in a cell? ... How I can extract the name of the sheet and pasted it in a cell ...

https://techcommunity.microsoft.com/t5/excel/how-i-can-extract-the-name-of-the-sheet-and-pasted-it-in-a-cell/td-p/3936199

Massachusetts Bay - Internal Wave Packets Extracted from SAR Imagery Binned in 1x1 minute grid cells , Data Set (DS) , National ... Massachusetts Bay - Internal Wave Packets Extracted from SAR Imagery Binned in 1x1 minute grid cells ... contains internal wave packets extracted from SAR imagery that were binned in 1x1 minute latitude/longitude polygon grid cells ... Statistics were calculated for each polygon grid cell---in particular, internal wave packet frequency, total internal wave ...

https://www.fisheries.noaa.gov/inport/item/39342/dmp

Species-Dependent Apoptosis by Gugulipid Extract of Ayurvedic Medicine Plant Commiphora mukul in Human Prostate Cancer Cells Is ... Gugulipid (GL), extract of Indian Ayurvedic medicinal plant Commiphora mukul, has been used to treat a variety of ailments. We ... normal human prostate epithelial cell line. ROS. reactive oxygen species. ANOVA. analysis of variance. ERK. extracellular ... Species-Dependent Apoptosis by Gugulipid Extract of Ayurvedic Medicine Plant Commiphora mukul in Human Prostate Cancer Cells Is ...

https://molpharm.aspetjournals.org/content/79/3/499

Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells * Gi-Chang Kim ... Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. ... Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid accumulation levels were ... Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and ...

https://foodandnutritionresearch.net/index.php/fnr/article/view/1237

Both extracts showed antiviral activity against acyclovir-sensitive and acyclovir-resistant HSV-1 and HSV-2. Our analyses show ... Both extracts showed antiviral activity against acyclovir-sensitive and -resistant HSV-1 and HSV-2. Our analyses show that ... Evaluation of the algae extracts as topical formulations in an animal model of HSV-1 skin infection significantly reduced the ... Taken together, these findings suggest that these algae extracts may be potential phytotherapeutics against HSVs and may be ...

https://www.frontiersin.org/articles/10.3389/fmicb.2020.02006/full

In vitro cell studies showed that PE-loaded electrospun nanofibers enhanced HaCaT cell growth, attachment, and proliferation, ... favoring cell migration towards the scratch area in the wound healing assay and allowing a complete wound closure after 72 h ... electrospun nanofibers loaded with two different concentrations of Pinus radiata bark extracts (PEs) were fabricated via ... Ku, C.S.; Mun, S.P. Effect of Pinus radiate bark extracts with different molecular weight distributions on cell growth of NIH/ ...

https://www.mdpi.com/2073-4360/14/12/2331

Extracting numbers from string field but omit some unnecessary cells using Python Parser of ArcMap Field Calculator?. Ask ... Extracting numbers and text from string field using Python Parser of ArcMap Field Calculator? ... Extracting numbers from string field using Python Parser of ArcMap Field Calculator? ... Extracting numbers from string field using Python Parser of ArcMap Field Calculator? ...

https://gis.stackexchange.com/questions/309485/extracting-numbers-from-string-field-but-omit-some-unnecessary-cells-using-pytho

Using human breast cancer cells and primary human mammary epithelial cells in vitro, Ray and colleagues found the mechanism of bitter melon extract significantly decreased proliferation, that is, cell growth and division, and induced death in breast cancer cells. (sciencedaily.com)
The in vitro antiproliferative activity of sloe ethanolic extracts (flower, leaf, and fruit), collected from three areas in Bosnia and Herzegovina, were investigated against human prostate cancer cell lines PC-3 and DU145 using MTT assay. (srce.hr)
Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis. (rupress.org)
To the best of our knowledge, there is no report on the protective effects of R. oldhamii leaf extract on non-alcoholic fatty liver disease (NAFLD) in vivo and in vitro . (medsci.org)
For the in vitro assay, HepG2 cells were treated with FFAs (oleate/palmitate = 2:1) with or without treatment with R. oldhamii leaf ethyl acetate (EtOAc) fraction to observe lipid accumulation using Nile red and oil red O stains. (medsci.org)
To study the possible anticancer and antiproliferative activities of ethanolic leaf extract of Thuja occidentalis (TO) on A549 non-small lung carcinoma cells in vitro . (jcimjournal.com)
This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models. (biomedcentral.com)
An in vitro evaluation of cytochrome P450 inhibition and P-glycoprotein interaction with goldenseal, Ginkgo biloba, grape seed, milk thistle, and ginseng extracts and their constituents. (nih.gov)
These persons total, had been reported in New York City (NYC) methods included an in vitro mouse neuroblastoma cell assay during the preceding 10 years (2000-2009). (cdc.gov)
The aims of this study were to evaluate the wound healing potential in vivo and the cytotoxic effects in vitro of Psidium guajava (Myrtaceae) leaf extract and commonly used corticosteroids. (bvsalud.org)
For the in vitro toxicity assay, each substance was applied to mucosa fibroblast cell cultures in conditioned media. (bvsalud.org)
In vitro, the guava extract caused a decrease in the cell viability and growth when compared to the control and corticosteroids. (bvsalud.org)
Although in a short-term cytotoxicity analysis, the guava extract reduced the cell population in vitro, while in vivo, the extract accelerated wound healing. (bvsalud.org)
As the crude extract possesses antileishmanial activity, the compounds were assayed for cytotoxic and antiparasitic activities in vitro in murine macrophage cells (raw 264.7 cells) and in Leishmania amazoniensis as well as L. braziliensis promastigotes. (lu.se)

Result of the extraction yield showed that distilled water produced the highest yield (10.58%) followed by methanol (6.55%) whereas acetone extracted the lowest yield (4.58%) from C. odontophyllum stem bark. (scirp.org)
In conclusion, acetone and methanol extracts from C. odontophyllum stem bark exhibited promising cytotoxic activity against human colorectal cancer cell line HCT 116 and paves way for further investigation in view of its mode of cancer cell death. (scirp.org)
Phenolic Compounds of Aqueous and Methanol Extracts of Hypsizygus tessellatus (brown and white var. (springer.com)
Since the World Health Organization has suggested the exploration of natural products for cancer management owing to the side effects of chemotherapy and irradiation on humans and breast cancer accounts for the highest number of cancer related deaths globally, this study has examined antiproliferative effects of the aqueous and methanol extracts of Hypsizygus tessellatus (brown and white var. (springer.com)
Furthermore, the phytochemical composition of the mushroom extracts were identified using mass spectroscopy (UPLC-QTOF/MS). The obtained results showed aqueous extracts of F. velutipes (Enoki) and white H. tessellatus (Bunapi shimeji) caps to possess higher antiodixant activities against DPPH (IC 50 = 0.202 and 0.573 mg/mL, respectively), and H 2 O 2 (IC 50 = 0.622 and 0.745 mg/mL, respectively) compared tothe methanol extracts. (springer.com)
Aqueous extracts of the mushrooms also showed better ferric reducing antioxidant power (FRAP) values against ferric ions compared to the methanol extracts. (springer.com)
M. officinalis bark was extracted using distilled water, 95% ethanol, and methanol, and it was then fermented by A. niger . (hindawi.com)
The fermented methanol extract exhibited the highest antityrosinase activity, total phenolic content, and antioxidant activity. (hindawi.com)
The fermented methanol extracts inhibited skin-aging-related enzymes such as collagenase, elastase, MMP-1, and MMP-2. (hindawi.com)
Hence, in general, methanol-extracted M. officinalis fermented by A. niger for 72 h has the most active antioxidant, skincare, or antiaging compounds for healthy food or cosmetics applications. (hindawi.com)

The preliminary study is aimed at evaluating the cytotoxic activity of crude extracts from the stem bark of Canarium odontophyllum against colorectal carcinoma cell HCT 116. (scirp.org)
Phytochemical screening test revealed the presence of terpenoid, flavonoid, tannin, saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. (scirp.org)
Acetone extract of C. odontophyllum stem bark showed significantly higher (p (scirp.org)
In this study, poly(ε-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of Pinus radiata bark extracts (PEs) were fabricated via electrospinning for wound healing applications. (mdpi.com)
This study intended to improve physiological characteristics of Magnolia officinalis bark (MOB) extracts by Aspergillus niger fermentation. (hindawi.com)
The French oak wood extract was launched last year by Horphag Research, the company behind the Pycnogenol brand of French Maritime Pine bark extracts. (nutraingredients.com)
In addition to the trichilianones A-D recently reported from Trichilia adolfi, a continuing investigation of the chemical constituents of the ethanol extract of the bark of this medicinal plant yielded the five new limonoids 1-5. (lu.se)

Standardized aqueous mistletoe extracts have been applied to cancer patients for several decades as complementary medicine. (iiarjournals.org)
Mistletoe extracts are one of the most widely studied complementary and alternative medicine therapies in people with cancer . (cancer.gov)
In Europe, mistletoe extracts are among the most prescribed drugs for patients with cancer (see Question 1 ). (cancer.gov)
Few side effects have been reported from the use of mistletoe extracts (see Question 5 ). (cancer.gov)
The U.S. Food and Drug Administration (FDA) has not approved mistletoe extracts as a treatment for cancer or any other medical condition (see Question 6 ). (cancer.gov)
Mistletoe extracts are made in water-based solutions or solutions of water and alcohol . (cancer.gov)
How are mistletoe extracts given? (cancer.gov)
Mistletoe extracts are usually given by an injection under the skin. (cancer.gov)
What laboratory or animal studies have been done using mistletoe extracts? (cancer.gov)
Laboratory and animal studies have tested the effects of mistletoe extracts. (cancer.gov)
For information on laboratory and animal studies using mistletoe extracts, see the Laboratory/Animal/Preclinical Studies in the health professional version of this summary. (cancer.gov)
Have any studies of mistletoe extracts been done in people with cancer? (cancer.gov)
Most clinical trials using mistletoe extracts to treat cancer have been done in Europe. (cancer.gov)

The two major components to which beneficial effects are related, namely, 2-methoxy-benzoquinone and 2,6-dimethoxy-benzoquinone [ 6 ], are found in high concentrations in these extracts. (hindawi.com)
The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. (ejbiotechnology.info)
Breast cancer cells were treated with varying concentrations (1 to 5 mg/ml) of Solanum nigrum extract for 24 and 48 h. (ijpsonline.com)
Different concentrations of Solanum nigrum extract showed cytotoxic effect on MDA-MB-468 cells. (ijpsonline.com)
In summary, our results demonstrate that A549 cells are insensitive to both low and high concentrations of green tea extract, likely due to the induction of cytoprotective autophagy. (bioinformation.net)
Treatment with GL significantly inhibited the viability of human prostate cancer cell line LNCaP (androgen-dependent) and its androgen-independent variant (C81) with an IC 50 of ∼1 μM (24-h treatment), at pharmacologically relevant concentrations standardized to its major active constituent z-guggulsterone. (aspetjournals.org)
Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. (foodandnutritionresearch.net)
Effects of Thuja occidentalis ethanolic extract on viability of A549 cell line (A) and L-132 cell line (B) Cells were exposed to Thuja occidentalis ethanolic extract for 24 h at different concentrations and the cell viability was determined by MTT assay. (jcimjournal.com)

We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. (nih.gov)
In addition, cell apoptosis was measured by double staining, DNA fragmentation and annexin V-fluorescein isothiocyanate binding assays. (ijpsonline.com)
Effects of Thuja occidentalis ethanolic extract on A549 cell migration (Light microscopy, ×20) Wound-healing assays were performed to assess cell migration. (jcimjournal.com)
Viability and radiation response of macrophages after exposure to tea extracts was measured by MTT assays. (biomedcentral.com)
In cytotoxicity assays, the A. niger- fermented extracts were nontoxic to CCD-966SK cells, even at 500 μ g/mL. (hindawi.com)

Cell sensitivity was evaluated using the MTT assay, while cell death was assessed using the Tali image-based cytometer. (bioinformation.net)
Cell viability was ascertained through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay after deployment of TO in different doses. (jcimjournal.com)
Effects of Thuja occidentalis ethanolic extract on cellular proliferation tested by BrdU incorporation assay Cells were exposed to three different concentration of Thuja occidentalis ethanolic extract for 6, 12 and 24 h along with incorporation of BrdU. (jcimjournal.com)
Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). (biomedcentral.com)
Cell proliferation was assessed by the trypan blue exclusion technique and the cytotoxicity of coumaric acid was performed using an LDH assay. (bvsalud.org)

In efforts to find natural substances that are less toxic than minoxidil and finasteride, previous studies have screened about 1,000 plant extracts for hair growth or hair loss-preventing effects [ 5 , 6 ]. (biomedcentral.com)

Assessment of autophagy function indicated that GTE-induced autophagy may serve as a self-protective mechanism against cytotoxicity, as blocking autophagy with bafilomycin A1 reduced cell viability and enhanced necrotic cell death in response to GTE treatment. (bioinformation.net)
TO demonstrated a dose-dependent decrease in viability of A549 cells after 24 h of exposure. (jcimjournal.com)
To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure. (hindawi.com)

Our findings suggest that bitter melon extract modulates several signal transduction pathways, which induces breast cancer cell death," said lead researcher Ratna B. Ray, Ph.D., professor in the Department of Pathology at Saint Louis University. (sciencedaily.com)
Treatment with S. occidentalis extracts and light induces an intense lamin A/C signal at the junction of a nuclear vacuole and the nucleus. (nih.gov)
We have developed a cell-free system that induces the morphological transformations characteristic of apoptosis in isolated nuclei. (rupress.org)

Evaluation of the algae extracts as topical formulations in an animal model of HSV-1 skin infection significantly reduced the severity of the disease more than acyclovir, as well as the duration of the herpetic lesions, when compared to mock-treated animals, with the D. antarctica extract performing best. (frontiersin.org)
Taken together, these findings suggest that these algae extracts may be potential phytotherapeutics against HSVs and may be useful for the treatment and reduction of common herpetic manifestations in humans. (frontiersin.org)
During the last decades, it has been shown that several biological systems including human cells, fungi, yeasts, bacteria, diatoms, algae, and plants can convert inorganic metal ions into metal NPs through reducing capacities. (degruyter.com)
Our commitment has been to test the capacity of some extracts of these algae to activate the defence mechanisms of plants as an alternative to pesticides, so that the plant is stronger when attacked by a pathogenic fungus," specified Zarraonaindia. (thefishsite.com)
We will be testing the algae extracts in vineyards in Jerez and La Rioja under local conditions," Zarraonaindia added. (thefishsite.com)

The highest activity against PC-3 and DU145 was gathered from leaf extracts obtained by different extraction methods (microwave-assisted extraction and ultrasound-assisted extraction). (srce.hr)
In this study, the effects of R. oldhamii leaf extract on inhibiting the free fatty acid (FFA)-induced accumulation of fat in HepG2 cells and on improving fatty liver syndrome in mice with high fat diet (HFD)-induced NAFLD were investigated. (medsci.org)
Following reverse-phase high-performance liquid chromatography (HPLC), four specific phytochemicals, including (2 R , 3 R )-astilbin (AS), hyposide (HY), guaijaverin (GU) and quercitrin (QU), were isolated and identified from the EtOAc fraction of R. oldhamii leaf extract. (medsci.org)
These results demonstrated that the methanolic extracts from R. oldhamii leaf have excellent inhibitory activities against fat accumulation and anti-NAFLD activities and thus have great potential as a natural health product. (medsci.org)
Scientists documented for the first time that papaya leaf extract boosts the production of key signaling molecules called Th1-type cytokines. (esciencenews.com)
In the microbiota on the leaf surface, we also saw that some fungi that help the plant in biological control are more abundant on the plants that received the Rugulopteryx extract. (thefishsite.com)
Ethanolic leaf extract of TO demonstrated apoptotic and antiproliferative potentials against A549 cell line. (jcimjournal.com)
The mixture of this quid, or chew, is a combination of the areca nut (fruit of the Areca catechu palm tree, erroneously termed betel nut) and betel leaf (from the Piper betel, a pepper shrub), tobacco, slaked lime (calcium hydroxide), and catechu (extract of the Acacia catechu tree). (medscape.com)

A previous study reported that the extract of the whole plant of SN activated cell death in hepatoma cells through autophagy and apoptosis [ 7 ]. (ijpsonline.com)
The system uses extracts prepared from mitotic chicken hepatoma cells following a sequential S phase/M phase synchronization. (rupress.org)

Walnut green husk ethanolic extract was obtained as 169.1 mg juglone/100 g Green Husk and antioxidant activity (ORAC) of 44,920 μmol Trolox Equivalent/100 g DW Green Husk. (ejbiotechnology.info)

Peripheral lymphocytes from three healthy nonsmoking donors were incubated with dichloromethane plus acetone or aqueous extracts of anthracite, bituminous, subbituminous coal, lignite, or peat dust or snuff alone or in combination. (cdc.gov)

The physiological characteristics of the fermented extracts, namely, tyrosinase inhibitory activity, antioxidant activity, antibacterial activity, and anti-skin-aging activity, were evaluated and compared with those of unfermented extracts. (hindawi.com)

The total phenolic content of the extracts fermented by A. niger was 3.52 times greater than that of the unfermented extracts. (hindawi.com)

Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. (ejbiotechnology.info)

Because of these effects, the extract is commonly used in folk medicines as a remedy for diabetes in locales such as India, China and Central America, according to the researchers. (sciencedaily.com)
We provide the first report of the effects of extracts prepared from the shrub Symphoricarpos occidentalis in several human cell lines. (nih.gov)
FWGE has significant antiproliferative effects and kills tumour cells by the induction of apoptosis via the caspase-poly (ADP-ribose) polymerase pathway [ 9 ]. (hindawi.com)
RD, Hep-2C, and BGM cells injected with an extract similarly processed but from healthy shrimp, showed no cytopathic effects, even after 7 days of culture ( Figures 1A , 1C , and 1 E ). As a positive control, RD cells were injected with Sabin viral extract and showed the characteristic cytopathic effect produced by an enterovirus infection. (cdc.gov)
Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. (ejbiotechnology.info)
Solanum nigrum (SN) is a herb with anticancer effects such as destruction of tumor cell membrane, induction of apoptosis by NF-kappaB, caspase activation, nitric oxide production and inhibition of angiogenesis [ 3 ]. (ijpsonline.com)
This study aimed to investigate the effects of green tea extract on A549 cells, a type of non-small lung cancer cells. (bioinformation.net)
The findings suggested that even at the highest dose tested (150 μM), GTE did not exhibit toxic effects on A549 cells. (bioinformation.net)
According to the JIB extract's anti-melanoma capacity, to assess the synergistic effects of cisplatin and JIB extract. (medsci.org)
Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)
Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. (foodandnutritionresearch.net)
Following the guidelines of "Good Clinical Practice" (GCP) this study was performed to get information on efficacy, safety and side-effects of the standardized mistletoe extract (sME). (iiarjournals.org)
In the present study, we aimed to investigate the effects of continuous intake of aqueous extract from I. obliquus on tumor suppression. (researchgate.net)
Key findings: The extract of I. obliquus caused significant tumor suppressive effects in both models. (researchgate.net)
We investigated the underlying molecular mechanisms and compared effects of tea extracts with the effects of epigallocatechin-gallate, the proposed most-active moiety of green tea. (biomedcentral.com)
Effects of tea extracts on 26S proteasome function were assessed. (biomedcentral.com)
NF-κB activity was altered by tea extracts in a complex, caspase-dependent manner, which differed from the effects of epigallocatechin-gallate. (biomedcentral.com)
In laboratory studies , a substance is tested in tumor cells to find out if it has any anticancer effects. (cancer.gov)
Tyrosinase inhibitors specifically interact with melanogenic cells and do not lead to side effects compared with other melanogenesis inhibitors [ 3 ]. (hindawi.com)
Therefore, exploring fermented herb extracts that have beneficial effects to prevent skin aging is important. (hindawi.com)
"Effects of [the] extract were observed mainly after stratification of total group of volunteers according to the level of feeling in the items at the pre-treatment questionnaire," they wrote. (nutraingredients.com)
The effects on cell cycling were determined. (cdc.gov)
Recently, cell protective, antiamnestic and antidepressant-like effects were disclosed but the possible application of RP to post-traumatic stress disorder, in which exaggerated fear memory persists, has not yet been explored. (lu.se)

No cytotoxic effect was observed by the aqueous extract despite its high extraction yield. (scirp.org)
In the recent research, silver nanoparticles (AgNPs) were formulated by the Panax ginseng aqueous extract. (degruyter.com)

Aug. 5, 2021 For a cell to grow and divide, it needs to produce new proteins. (sciencedaily.com)
Three heat precipitation methods are presented that effectively remove more than 90% of host cell proteins (HCPs) from tobacco extracts prior to any other purification step. (jove.com)
The overall goal of this set of experiments is to precipitate plant host cell proteins using heat. (jove.com)
To heat precipitate host cell proteins. (jove.com)
Autophagy, the type II cell death, is a physiological mechanism involving degradation of intracellular damaged proteins and organelles into membrane vacuoles. (ijpsonline.com)
This cellular "realization," however, requires healthy cell communication, and that's dependent on the correct nutrients, minerals and proteins being available in the body. (infiniteunknown.net)
Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
The induction of proapoptotic Bcl-2 family proteins Bax and Bak and a decrease of antiapoptotic Bcl-2 protein Bcl-2 were observed in GL-treated cells. (aspetjournals.org)
Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. (foodandnutritionresearch.net)
Our analyses show that there is a significant antiviral activity associated with proteins in the extract, although other compounds also seem to contribute to inhibiting the replication cycle of these viruses. (frontiersin.org)
Aside from the part of the grape it comes from, resveratrol is different from grape seed extract in that it activates unique genes-sometimes called our "longevity genes"-which encode the instructions for the body to create proteins called sirtuins. (lifeextension.com)
Yeast cells are heated until they rupture, then the cells' own digestive enzymes break their proteins down into simpler compounds (amino acids and peptides), a process called autolysis. (wikipedia.org)

A549 cells treated with green tea extract (GTE) were examined using an inverted light microscope and a fluorescence microscope. (bioinformation.net)

Combined treatment of FWGE and 5-fluorouracil or dacarbazine resulted in a synergistic effect in the HCR-25 human cancer cell line and B16 murine melanoma cell line [ 6 ]. (hindawi.com)
In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)

However, linear DNA is rapidly degraded by exonucleases naturally present in cellular extracts 4 . (jove.com)
The cytopathic effect in RD cells began with a partial destruction of the cellular layer. (cdc.gov)
Next, small cellular groups and some isolated round cells were observed. (cdc.gov)
In Hep-2C cells, the cellular monolayer was partially destroyed. (cdc.gov)
The cytopathic effect in the BGM cell line began as a partial destruction of the cellular layer, which evolved to a syncytial-like formation of rounded, refringent cells. (cdc.gov)
Autophagy and apoptosis are two forms of programmed cell death, which are essential for cellular homeostasis. (ijpsonline.com)
It is interesting to note that a representative normal prostate epithelial cell line (PrEC) was relatively more resistant to GL-mediated cellular responses compared with prostate cancer cells. (aspetjournals.org)

If the results demonstrated in the labs at the University of Kentucky can be replicated in humans, it could potentially position grapeseed extract as one of the most powerful natural chemotherapeutic agents yet discovered. (infiniteunknown.net)
Systemic exposure to Ginkgo biloba extract in male F344/NCrl rats: Relevance to humans. (nih.gov)

Sloe ( Prunus spinosa L.) extracts are a good source of natural bioactive compounds, including phytosterols. (srce.hr)
Doing so may allow specific compounds to be extracted or to produce an extract without the hydrolysis of cell contents (as in autolysis) happening. (wikipedia.org)

The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
Cell proliferation was reduced in a time-dependent manner of drug exposure as revealed from BrdU incorporation and migration studies. (jcimjournal.com)
Coumaric acid from M. polymorphum extracts reverses the activated state of hepatic stellate cells (GRX) and inhibits their proliferation by decreasing the p53/p21 pathway. (bvsalud.org)
The experiments performed showed that the treatment with coumaric acid was able to reduce cell proliferation without causing cell cytotoxicity or apoptosis . (bvsalud.org)

In this study, the protective effect of a fermented wheat germ extract (FWGE) against LPS-induced inflammation and oxidative stress in IPEC-J2 porcine intestinal epithelial cells was studied. (hindawi.com)
Decreases rhinovirus-induced up-regulation in respiratory epithelial cells and modulate pretranscriptional mechanisms. (medscape.com)

A fermented wheat germ extract (FWGE) is a natural nontoxic substance which is already used in the human medicine as a supportive therapy in cancer patients under radiotherapy and chemotherapy [ 5 ]. (hindawi.com)

Another important finding in the University of Kentucky study is that the grapeseed extract was not toxic to normal, healthy cells even as it helped cancer cells commit suicide. (infiniteunknown.net)
Conventional chemotherapy chemicals are extremely toxic to healthy cells, which is why chemotherapy causes permanent brain damage ("chemo brain"), kidney damage and heart damage. (infiniteunknown.net)
Papaya extract seems to have a toxic effect on cancer cells in culture, suggesting a potential treatment. (esciencenews.com)

Further study of S. occidentalis extracts and vacuolation provide chemical tools that may contribute to the understanding of nuclear envelope organization and human cell biology. (nih.gov)
Here we demonstrate that TSV also infects human cell lines, which may suggest that Penaeus is a potential reservoir of this virus. (cdc.gov)
Since Sabin strain LSc 2ab (Sabin 1), the poliovirus used for human vaccination, is usually replicated in monolayer culture cells of human rhabdomyosarcoma (RD), human larynx carcinoma (Hep-2C) (), or Buffalo green monkey kidney (BGM) ( 7 ), we injected these cell lines with a 0.22-µm membrane-filtered whole extract of the hepatopancreas of shrimp ( Penaeus stylirostris ) affected with TSV. (cdc.gov)
Microscopic image of the subcuticular tissue of the pleopod from a shrimp infected with the supernatant of the third passage of a human larynx carcinoma (Hep-2C) cell culture inoculated with an. (cdc.gov)
There are hundreds or thousands of "microtumors" in every human being living today, but cancerous cells in healthy people destroy themselves once they realize they're flawed. (infiniteunknown.net)
It's important to note that this recent study was conducted in a lab, not in human beings, so its conclusions cannot necessarily be directly translated into saying something like "grapeseed extract cures cancer," for example. (infiniteunknown.net)
We report an anticancer effect and mechanism of GL against human prostate cancer cells. (aspetjournals.org)
Nuclear lamina disassembly accompanies these structural changes in added nuclei, and we show that lamina disassembly is a characteristic feature of apoptosis in intact cells of mouse, human and chicken. (rupress.org)
Finally, the mushroom extracts showed good antiproliferative activities against human breast cancer cell lines. (springer.com)
Tea extracts were compared for their ability to modulate IL-1β, IL-6, IL-8, TNFα and PGE 2 release from human monocytes. (biomedcentral.com)
GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 μM minoxidil. (biomedcentral.com)
Serially diluted (1/500, 1/1000 and 1/2000) serum from each subject as well as human IgG standards (2000 ng/ml, 1000 ng/ml, 500 ng/ml and 250 ng/ml) are loaded perpendicularly to the bacterial extracts, and are allowed to interact. (cdc.gov)
Induction of sister chromatid exchanges by coal dust and tobacco snuff extracts in human peripheral lymphocytes. (cdc.gov)

Compared with the unfermented extracts, the fermented extracts also contained greater antibacterial activity against tested stains including MRSA . (hindawi.com)

Unique microRNA alterations in hepatocellular carcinomas arising either spontaneously or due to chronic exposure to Ginkgo biloba extract (GBE) in B6C3F1/N mice. (nih.gov)

Bitter melon extract, a common dietary supplement, exerts a significant effect against breast cancer cell growth and may eventually become a chemopreventive agent against this form of cancer, according to results of a recent study. (sciencedaily.com)
Agarwal gave a note of caution, stating that while these results do provide hope as an anti-cancer agent, it is important to establish the validity of these results in animal models before adding them to one's diet to inhibit breast cancer cell growth. (sciencedaily.com)
The results of the analysis proved the presence of phytosterols, mostly β-sitosterol in all extracts. (srce.hr)
To the best of our knowledge, no other studies have presented results on antiproliferative activity of ethanol sloe extracts. (srce.hr)
Based on these results, further investigation should be recommended on other cancer cell lines as well. (srce.hr)
Our results indicated the anti-melanoma activity of JIB extract. (medsci.org)
Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
The most positive results were obtained with one of the extracts produced from the invasive alga Rugulopteryx okamurae ," explained Zarraonaindia. (thefishsite.com)
In clinical research, grape seed extract has led to some improvements in cognitive function, but the results are mixed, therefore more research is needed. (lifeextension.com)
Results from the study showed that patients treated with mistletoe extract had improved survival compared with those who did not receive mistletoe extract. (cancer.gov)

These findings indicated that Solanum nigrum extract induced cell death in MDA-MB-468 cells by two distinct mechanisms, apoptosis and autophagy, and these findings further suggest a possibility that this extract could be used to treat triple negative breast cancer. (ijpsonline.com)
These findings suggest the presence of phytochemicals with antiproliferative and antioxidant acrtivities in the mushroom extracts studied. (springer.com)
Significance: Our findings suggest that the I. obliquus extract could be used as a natural remedy for cancer suppression by promoting energy metabolism. (researchgate.net)

These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation and DNA fragmentation [ 14 ]. (ijpsonline.com)
When nuclei are added to these extracts, the chromatin becomes highly condensed into spherical domains that ultimately extrude through the nuclear envelope, forming apoptotic bodies. (rupress.org)

Main methods: Anticancer activity of the I. obliquus extract was examined in mouse models of Lewis lung carcinoma growth and spontaneous metastasis after 3 weeks of continuous extract intake at the dose of 6 mg/kg/day, which corresponded to that ingested daily with Chaga infusion in Japan. (researchgate.net)
Patients received best supportive care and were randomly assigned to receive either mistletoe extract or no anticancer therapy . (cancer.gov)

A retrospective cohort study done between 1993 and 2000 looked at the use of mistletoe extract as long-term adjuvant therapy in patients treated with chemotherapy and/or radiation therapy for colorectal cancer that had not spread. (cancer.gov)
The study found that patients treated with mistletoe extract had longer disease-free survival , fewer adverse events, and better symptom relief than patients who did not receive mistletoe extract as adjuvant therapy. (cancer.gov)

Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)

Mast cell stabilizers inhibit mast cell degranulation and influence granulocyte chemotaxis. (medscape.com)
Coumaric acid was able to inhibit the expression of cyclin D1 and CDK's (CDK2, CDK4, and CDK6), increasing p53 and p21, which could lead to cell cycle arrest . (bvsalud.org)

Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 ( ΔrecBCD and ΔrecB ). (jove.com)

One of the hallmarks of breast cancer is resistance of tumor cells to cell death. (ijpsonline.com)
Develop a logical model describing the interplay between Bispecific T-cell engager antibodies (BiAbs) the immune system, and tumor cells. (cosbi.eu)

Vacuolated cells show a striking phenotype of a large perinuclear vacuole (nuclear associated vacuole, NAV) that is distinct from vesicles observed by treatment with an autophagy-inducing agent. (nih.gov)
Treatment with coumaric acid was also able to revert the activated phenotype of GRX cells to their quiescent state . (bvsalud.org)

However, it does indicate quite convincingly that if the unique phytochemical molecules found in grapeseed extract can be delivered to leukemia cells with sufficient potency, they may play an important role in cancer cells destroying themselves, thereby protecting the whole organism from runaway cancer. (infiniteunknown.net)

Likewise, treatment with GTE resulted in a minimal, dose-dependent increase in the population of apoptotic cells. (bioinformation.net)
The highest combined dose induced cell cycle delay in all three donors. (cdc.gov)
Male mice received daily oral dose of RP extract or vehicle during the SPS-CF procedure. (lu.se)

A Japanese mushroom extract was shown to eradicate HPV in cervical cancer cells as well as mouse models of the infection. (medicaldaily.com)

Organic solvent extracts of bituminous and subbituminous coal dust were tested with lymphocytes from five other donors to assess variability in the responses. (cdc.gov)

Bitter melon extract is cultivated in Asia, Africa and South America. (sciencedaily.com)
In Africa, the highest prevalence of sickle-cell trait occurs between latitudes 15° North and 20° South, ranging between 10% and 40% of the population in some areas ( Figure 1 ). (who.int)

Polyphenols, including catechins from green tea extract, have long been known for their potential anti-tumour activities. (bioinformation.net)

If you want to benefit from the polyphenols from wine but want to avoid the alcohol (and calories), then a grape seed extract supplement may be a "fruitful" addition to your health routine. (lifeextension.com)
What is grape seed extract? (lifeextension.com)
Just as the name implies, grape seed extract is an extract from the seed (and not the fruit) of grapes. (lifeextension.com)
Grape Seed Extract vs. Resveratrol: What's the Difference? (lifeextension.com)
Resveratrol indeed is a good reason to eat grapes, but it's not the same thing as grape seed extract, nor does it have the exact same benefits. (lifeextension.com)
Grape seed extract comes from the seeds of the grape, whereas resveratrol is found in the skin of the grape. (lifeextension.com)
On the other hand, grape seed extract has a high concentration of vitamin E and proanthocyanidins, which means it may offer other benefits that resveratrol does not. (lifeextension.com)
Drinking wine in moderation is associated with many health benefits, but taking a grape seed extract is an alcohol-free way to possibly obtain them. (lifeextension.com)
Grape seed extract may help support healthy blood pressure levels by boosting your levels of nitric oxide , which dilates blood vessels. (lifeextension.com)
In addition, grape seed extract may support blood pressure levels already in the normal range by making it easier for you to keep a handle on stress (because it's not a myth that these two are related! (lifeextension.com)
An interesting study found that 14 days of daily intake of a grape seed extract supplement improved leg comfort in women who sat for prolonged periods of time. (lifeextension.com)
Preclinical research has shown that the proanthocyanidins in grape seed extract promote basal synaptic transmission in the part of the brain known as the hippocampus, which is intimately involved in memory. (lifeextension.com)
Multiple preclinical studies have shown that grape seed proanthocyanidins support healthy cell division and growth. (lifeextension.com)

All these methods require supplementations to the cell extract, which are costly and time-consuming. (jove.com)
The type of extract used and if it is made with homeopathic methods. (cancer.gov)
Although the vast majority of yeast extract spreads are made using von Liebig's traditional approach of heat-autolysis using surplus yeast from beer brewing, other methods do exist for producing specialized types. (wikipedia.org)
For disruption of the cell, some physical and chemical methods may be used in place of the heat-autolysis process. (wikipedia.org)

Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. (ejbiotechnology.info)

Tea extracts inhibited proteasome function and suppressed cytokine release. (biomedcentral.com)

Ray and colleagues are currently conducting follow-up studies using a number of cancer cell lines to examine the anti-proliferative effect of the extract. (sciencedaily.com)
Metabolites 1-3 and 5 showed moderate cytotoxicity (between 30-94 μg/mL) but are not responsible for the antileishmanial effect of the extract. (lu.se)

2023). 'HPLC Analysis of Phytosterols in Prunus spinosa L. Extracts and Their Antiproliferative Activity on Prostate Cancer Cell Lines', Kemija u industriji , 72(5-6 (special issue)), str. (srce.hr)

Organic anthracite extracts did not induce SCEs. (cdc.gov)
The subbituminous dust extract did not induce SCEs. (cdc.gov)

Astragalus contains antioxidants, which can potentially prevent cell damage. (msdmanuals.com)

All extracts possess antiproliferative activity. (srce.hr)
Both extracts showed antiviral activity against acyclovir-sensitive and acyclovir-resistant HSV-1 and HSV-2. (frontiersin.org)
After treatment with this extract, we saw how there was an increase in the expression of resistant genes and in the activity of antioxidant enzymes in the plant, among other things. (thefishsite.com)
may decrease number and activity of inflammatory cells, decreasing airway hyper-responsiveness. (medscape.com)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

Rheumatoid arthritis (RA) is an autoimmune and inflammatory disease that occurs when a person's immune system attacks healthy cells in the body. (medicaldaily.com)
In vivo, guava extract caused accelerated wound healing from the 3rd day, whereas the corticosteroids delayed tissue repair and were associated with bacterial surface colonization, the presence of micro-abscesses and intense inflammatory infiltrate in the submucosa. (bvsalud.org)

Therefore, remedies based on the induction of programmed cell death attracted greater attention for cancer treatment [ 8 ]. (ijpsonline.com)
Mean ± SD scores for lameness (A), joint flexion (B), and joint effusion (C) in horses that had undergone experimental induction of osteoarthritis in 1 middle carpal joint and a sham operation in the other middle carpal joint, followed by oral treatment with ASU extracts (1:2 [avocado-to-soybean] ratio mixed in 6 mL of molasses) or molasses (6 mL) alone (placebo treatment) once daily from days 0 to 70. (avma.org)
The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. (biomedcentral.com)

Agave Stem Cell Extract can slow skin aging by defending against extrinsic stress, keeping skin looking youthful, longer. (ultrapurecosmetics.com)
Expression of Ki-67 and stem cell factor were analyzed by immunohistochemistry. (biomedcentral.com)
Stem cells and gene therapy may be used in the future. (medlineplus.gov)
Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties -- self renewal and pluripotency. (lu.se)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)

Treatment with FWGE (1-2%) significantly decreased the level of intracellular ROS compared to LPS-treated cells. (hindawi.com)
Upregulation of Bax and downregulation of Bcl2 was detected after treatment with 5 mg/ml of Solanum nigrum extract. (ijpsonline.com)
Tea extracts are an efficient, broadly available treatment option for patients suffering from acute radiation-induced skin toxicity. (biomedcentral.com)
A study in postoperative early-stage breast cancer patients compared standard treatment and mistletoe extract to standard treatment alone. (cancer.gov)
GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues. (biomedcentral.com)

Impaired translesion synthesis in xeroderma pigmentosum variant extracts. (1/1728)

Mutations of oncoprotein 18/stathmin identify tubulin-directed regulatory activities distinct from tubulin association. (2/1728)

Regulation of human hsp90alpha gene expression. (3/1728)

unr, a cellular cytoplasmic RNA-binding protein with five cold-shock domains, is required for internal initiation of translation of human rhinovirus RNA. (4/1728)

Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ oscillation-inducing sperm factor. (5/1728)

Discrete regional distributions suggest diverse functional roles of calcium channel alpha1 subunits in sperm. (6/1728)

Defective repair of cisplatin-induced DNA damage caused by reduced XPA protein in testicular germ cell tumours. (7/1728)

Bcl-2 regulates a caspase-3/caspase-2 apoptotic cascade in cytosolic extracts. (8/1728)

Yeast extract

Grape seed extract

Buffered charcoal yeast extract agar

Rwanda-Spain relations

Anemonin

David Sidney Feingold

Spliceosome

Future Meat Technologies

Cell-free protein synthesis

Thomas B. Kornberg

Ethanolamine ammonia-lyase

Sanguinaria

Methenyltetrahydromethanopterin cyclohydrolase

Rhus ovata

Nucleoplasmin

Herman Kalckar

Cell cycle checkpoint

Micrococcal nuclease

Portulaca pilosa

Karenia mikimotoi

Antinuclear antibody

Hymenaea stigonocarpa

Fucoidan

Limosilactobacillus reuteri

Cellular waste product

Galactosaminogalactan

Lymphocyte T-cell immunomodulator

Single-cell variability

G2-M DNA damage checkpoint

Biological response modifier

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Vitro14

Methanol10

Bark7

Mistletoe extracts13

Concentrations8

Assays5

Assay5

Plant Extracts1

Viability3

Induces3

Algae5

Leaf8