Branched DNA Signal Amplification Assay
Nucleic Acid Amplification Techniques
Self-Sustained Sequence Replication
Sensitivity and Specificity
Encyclopedias as Topic
Nucleic Acids
Polymerase Chain Reaction
Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma. (1/39)
The performance and characteristics of Roche COBAS AMPLICOR HIV-1 MONITOR version 1.5 (CA MONITOR 1.5) UltraSensitive (usCA MONITOR 1. 5) and Standard (stCA MONITOR 1.5) procedures, Organon Teknika NucliSens HIV-1 RNA QT with Extractor (NucliSens), and Bayer Quantiplex HIV RNA version 3.0 (bDNA 3.0) were compared in a multicenter trial. Samples used in this study included 460 plasma specimens from human immunodeficiency virus (HIV) type 1 (HIV-1)-infected persons, 100 plasma specimens from HIV antibody (anti-HIV)-negative persons, and culture supernatants of HIV-1 subtype A to E isolates diluted in anti-HIV-negative plasma. Overall, bDNA 3.0 showed the least variation in RNA measures upon repeat testing. For the Roche assays, usCA MONITOR 1.5 displayed less variation in RNA measures than stCA MONITOR 1.5. NucliSens, at an input volume of 2 ml, showed the best sensitivity. Deming regression analysis indicated that the results of all three assays were significantly correlated (P < 0.0001). However, the mean difference in values between CA MONITOR 1.5 and bDNA 3.0 (0.274 log(10) RNA copies/ml; 95% confidence interval, 0.192 to 0.356) was significantly different from 0, indicating that CA MONITOR 1.5 values were regularly higher than bDNA 3.0 values. Upon testing of 100 anti-HIV-negative plasma specimens, usCA MONITOR 1.5 and NucliSens displayed 100% specificity, while bDNA 3.0 showed 98% specificity. NucliSens quantified 2 of 10 non-subtype B viral isolates at 1 log(10) lower than both CA MONITOR 1.5 and bDNA 3.0. For NucliSens, testing of specimens with greater than 1,000 RNA copies/ml at input volumes of 0.1, 0.2, and 2.0 ml did not affect the quality of results. Additional factors differing between assays included specimen throughput and volume requirements, limit of detection, ease of execution, instrument work space, and costs of disposal. These characteristics, along with assay performance, should be considered when one is selecting a viral load assay. (+info)Comparative performance of three viral load assays on human immunodeficiency virus type 1 (HIV-1) isolates representing group M (subtypes A to G) and group O: LCx HIV RNA quantitative, AMPLICOR HIV-1 MONITOR version 1.5, and Quantiplex HIV-1 RNA version 3.0. (2/39)
The performance of the LCx HIV RNA Quantitative (LCx HIV), AMPLICOR HIV-1 MONITOR version 1.5 (MONITOR v1.5), and Quantiplex HIV-1 RNA version 3.0 (bDNA v3.0) viral load assays was evaluated with 39 viral isolates (3 A, 7 B, 6 C, 4 D, 8 E, 4 F, 1 G, 4 mosaic, and 2 group O). Quantitation across the assay dynamic ranges was assessed using serial fivefold dilutions of the viruses. In addition, sequences of gag-encoded p24 (gag p24), pol-encoded integrase, and env-encoded gp41 were analyzed to assign group and subtype and to assess nucleotide mismatches at primer and probe binding sites. For group M isolates, quantification was highly correlated among all three assays. In contrast, only the LCx HIV assay reliably quantified group O isolates. The bDNA v3.0 assay detected but consistently underquantified group O viruses, whereas the MONITOR v1.5 test failed to detect group O viruses. Analysis of target regions revealed fewer primer or probe mismatches in the LCx HIV assay than in the MONITOR v1.5 test. Consistent with the high level of nucleotide conservation is the ability of the LCx HIV assay to quantify efficiently human immunodeficiency virus type 1 group M and the genetically diverse group O. (+info)Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0. (3/39)
Normal assay variation associated with bDNA tests for human immunodeficiency virus type 1 (HIV-1) RNA performed at two laboratories with different levels of test experience was investigated. Two 5-ml aliquots of blood in EDTA tubes were collected from each patient for whom the HIV-1 bDNA test was ordered. Blood was stored for no more than 4 h at room temperature prior to plasma separation. Plasma was stored at -70 degrees C until transported to the Central Pennsylvania Alliance Laboratory (CPAL; York, Pa.) and to the Hershey Medical Center (Hershey, Pa.) on dry ice. Samples were stored at < or =-70 degrees C at both laboratories prior to testing. Pools of negative (donor), low-HIV-1-RNA-positive, and high-HIV-1-RNA-positive plasma samples were also repeatedly tested at CPAL to determine both intra- and interrun variation. From 11 August 1999 until 14 September 2000, 448 patient specimens were analyzed in parallel at CPAL and Hershey. From 206 samples with results of > or =1,000 copies/ml at CPAL, 148 (72%) of the results varied by < or =0.20 log(10) when tested at Hershey and none varied by >0.50 log(10). However, of 242 specimens with results of <1,000 copies/ml at CPAL, 11 (5%) of the results varied by >0.50 log(10) when tested at Hershey. Of 38 aliquots of HIV-1 RNA pool negative samples included in 13 CPAL bDNA runs, 37 (97%) gave results of <50 copies/ml and 1 (3%) gave a result of 114 copies/ml. Low-positive HIV-1 RNA pool intrarun variation ranged from 0.06 to 0.26 log(10) while the maximum interrun variation was 0.52 log(10). High-positive HIV-1 RNA pool intrarun variation ranged from 0.04 to 0.32 log(10), while the maximum interrun variation was 0.55 log(10). In our patient population, a change in bDNA HIV-1 RNA results of < or =0.50 log(10) over time most likely represents normal laboratory test variation. However, a change of >0.50 log(10), especially if the results are >1,000 copies/ml, is likely to be significant. (+info)Quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in cell-free cervicovaginal secretions: comparison of reverse transcription-PCR amplification (AMPLICOR HIV-A MONITOR 1.5) with enhanced-sensitivity branched-DNA assay (Quantiplex 3.0). (4/39)
Two commercially available hypersensitive assays for human immunodeficiency virus type 1 (HIV-1) RNA quantitation, AMPLICOR HIV-1 Monitor Test 1.5 and Quantiplex HIV RNA 3.0, were compared to detect and quantify HIV-1 RNA in the cell-free fraction of cervicovaginal secretions collected by vaginal washing. Three panel specimens were used: pooled cervicovaginal secretions spiked with HIV-1 subtype A or HIV-1 subtype B and cervicovaginal lavages from HIV-positive and HIV-negative women. Compared to the AMPLICOR HIV-1 Monitor Test 1.5 assay, the Quantiplex HIV-1 3.0 assay yielded higher estimates of HIV-1 RNA concentrations in several tested samples spiked with HIV-1 RNA subtype A, as well as subtype B, particularly samples containing low amounts of HIV-1 RNA. The sensitivity and specificity of the AMPLICOR HIV-1 Monitor Test 1.5 assay were 93 and 100%, respectively; the sensitivity and specificity of the Quantiplex HIV RNA 3.0 assay were 97 and 50%, respectively. In conclusion, in quantifying HIV-1 RNA in cervicovaginal secretions, the Quantiplex HIV RNA 3.0 may lack specificity, and the AMPLICOR HIV-1 Monitor Test 1.5 assay, although highly specific, may lack sensitivity. (+info)Comparative evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR version 2.0 Assays for quantification of hepatitis C virus RNA in serum. (5/39)
A comparison of quantitative results expressed in hepatitis C virus (HCV) international units per milliliter, obtained from the VERSANT HCV RNA 3.0 (bDNA-3.0) assay, the QUANTIPLEX HCV RNA 2.0 (bDNA-2.0) assay, and the COBAS AMPLICOR HCV MONITOR version 2.0 (HCM-2.0) test was performed. A total of 168 patient specimens submitted to the Mayo Clinic Molecular Microbiology Laboratory for HCV quantification or HCV genotyping were studied. Of the specimens tested, 97, 88, and 79% yielded quantitative results within the dynamic range of the bDNA-3.0, bDNA-2.0, and HCM-2.0 assays, respectively. Overall, there was substantial agreement between the results generated by all three assays. A total of 15 out of 29 (52%) of the specimens determined to contain viral loads of <31,746 IU/ml by the bDNA-3.0 assay were categorized as containing viral loads within the range of 31,746 to 500,000 IU/ml by the bDNA-2.0 assay. Although substantial agreement was noted between the results generated by the bDNA-2.0 and bDNA-3.0 assays, a bias toward higher viral titer by the bDNA-2.0 assay was noted (P = 0.001). Likewise, although substantial agreement was noted between the results generated by the HCM-2.0 and bDNA-3.0 assays, a bias toward higher viral titer by the bDNA-3.0 assay was noted (P < or = 0.001). The discrepancy between the HCM-2.0 and bDNA-3.0 results was more pronounced when viral loads were >500,000 IU/ml and resulted in statistically significant differences (P < or = 0.001) in determining whether viral loads were above or below 800,000 IU/ml of HCV RNA, the proposed threshold value for tailoring the duration of combination therapy. The expression of quantitative values in HCV international units per milliliter was a strength of both the bDNA-3.0 and HCM-2.0 assays. (+info)Postnatal expression and induction by pregnenolone-16alpha-carbonitrile of the organic anion-transporting polypeptide 2 in rat liver. (6/39)
Newborn rats are more sensitive to the toxic effects of cardiac glycosides than are adult rats. This is associated with a decreased ability to remove cardiac glycosides from blood into the liver. Pregnenolone-16alpha-carbonitrile (PCN), a prototypical rodent CYP3A inducer and pregnane-X-receptor (PXR) ligand, stimulates the hepatic clearance of cardiac glycosides in newborn rats, which results in decreased toxicity of the cardiac glycosides. The mechanism responsible for this phenomenon is not clear; however, if elucidated, it would help in understanding and preventing potential drug-drug interactions. The recently cloned rat organic anion-transporting polypeptide 2 (oatp2) (Slc21a5) is a sinusoidal hepatic uptake transporter, with very high affinities for cardiac glycosides, and thus it was hypothesized that rat oatp2 increases during postnatal development and is inducible by PCN. In the present study, livers were removed from Sprague-Dawley rats from postnatal days (pnd) 0 to 45, in 5-day increments; as well as from pnd 10 to 90, in 10-day increments, after PCN (75 mg/kg i.p., for 4 days) or corn oil (vehicle for PCN) treatment. The protein and mRNA levels of rat oatp2 were determined by Western blot analysis and branched DNA signal amplification technique, respectively. Expression of rat oatp2 protein and mRNA increased gradually during postnatal development. PCN treatment increased liver to body weight ratio in both genders, and dramatically accelerated the maturation of hepatic oatp2 protein and mRNA levels. In summary, rat oatp2 undergoes age-dependent and chemical regulation during postnatal development, and is a potential target for drug-drug and age-drug interactions. (+info)Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum. (7/39)
We assessed the performance of a new assay (VERSANT HCV RNA 3.0 [bDNA 3.0] assay [Bayer Diagnostics]) to quantitate HCV RNA levels and compared the results of the bDNA 3.0 assay to results of the Quantiplex HCV RNA 2.0 (bDNA 2.0) assay. Samples used in this study included 211 serum specimens from hepatitis C virus (HCV)-infected persons from two sites (Bordeaux and Marseille, France) with different genotypes; 383 serum specimens from HCV antibody-negative, HCV RNA-negative persons; and serial dilutions of World Health Organization (WHO) HCV RNA standard at a titer of 100,000 IU/ml. The specificity of the bDNA 3.0 assay was 98.2%. A high correlation was observed between expected and observed values in all dilutions of WHO standard (r = 0.9982), in serial dilutions of pooled samples (r = 0.9996), and in diluted sera from different HCV genotypes (r = 0.9930 to 0.9995). The standard deviations (SD) for the within-run and between-run reproducibility of the bDNA 3.0 assay wereExternal quality assessment program for qualitative and quantitative detection of hepatitis C virus RNA in diagnostic virology. (8/39)
To assess the performance of laboratories in detecting and quantifying hepatitis C virus (HCV) RNA levels in HCV-infected patients, we distributed two proficiency panels for qualitative and quantitative HCV RNA testing. The panels were designed by the European Union Quality Control Concerted Action, prepared by Boston Biomedica Inc., and distributed in May 1999 (panel 1) and February 2000 (panel 2). Each panel consisted of two negative samples and six positive samples, with HCV RNA target levels from 200 to 500,000 copies/ml. Panel 1 had four samples with at least 50,000 copies/ml, and panel 2 had two samples with at least 50,000 copies/ml. Fifty-seven laboratories submitted 45 qualitative and 35 quantitative data sets on panel 1, and 81 laboratories submitted 75 qualitative and 48 quantitative data sets on panel 2. In both panels, about two-thirds of the qualitative data sets and >90% of the quantitative data sets were obtained with commercial assays. With each panel, two data sets gave one false-positive result, corresponding to false-positivity rates of 1.3% and 0.8% for panel 1 and panel 2, respectively. Samples containing at least 50,000 copies/ml were found positive in 97% and 99% of the cases with panel 1 and panel 2, respectively. In contrast, the positive samples containing < or =5,000 copies/ml were reported positive in only 71% and 77% of the cases with panel 1 and panel 2, respectively. Adequate or better scores on qualitative results (all results correct or only the low-positive samples missed) were obtained in 84% (panel 1) and 80% (panel 2) of the data sets. In the analysis of quantitative results, 60% (panel 1) and 73% (panel 2) of the data sets obtained an adequate or better score (> or =80% of the positive results within the range of the geometric mean +/- 0.5 log(10)). Our results indicate that considerable improvements in molecular detection and quantitation of HCV have been achieved, particularly through the use of commercial assays. However, the lowest detection levels of many assays are still too high, and further standardization is still needed. Finally, this study underlines the importance of proficiency panels for monitoring the quality of diagnostic laboratories. (+info)A Branched DNA (bDNA) Signal Amplification Assay is a medical diagnostic technique used to detect and quantify specific nucleic acid sequences, such as viral RNA or DNA. This method utilizes a series of hybridization and amplification steps to produce a measurable signal that is proportional to the amount of target nucleic acid present in a sample.
The bDNA assay involves several key components:
1. Probe Set: A set of synthetic oligonucleotides (DNA or RNA) designed to selectively bind to the target nucleic acid sequence. These probes are modified with unique sequences, called "branches," that serve as attachment points for additional probes in subsequent steps.
2. Amplifier Probes: A series of branched DNA molecules that hybridize to the probe set and contain multiple reporter molecules (e.g., enzymes or fluorophores) at their termini. These amplifier probes enhance the sensitivity of the assay by increasing the number of detectable signal molecules per target nucleic acid sequence.
3. Labeling Probes: Oligonucleotides that hybridize to the amplifier probes and contain a detectable label, such as a chemiluminescent or fluorescent moiety. These probes generate the measurable signal during the final step of the assay.
4. Signal Detection: The detection and quantification of the labeled probes can be performed using various methods, depending on the type of label used. For example, chemiluminescence or fluorescence can be measured using a luminometer or a fluorimeter, respectively.
The bDNA Signal Amplification Assay offers several advantages over other nucleic acid detection techniques, including its ability to detect low levels of target nucleic acids and its compatibility with a wide range of sample types (e.g., serum, plasma, tissue). However, it is generally more complex and time-consuming than alternative methods like PCR or real-time qPCR.
This technique has been widely used for the detection and quantification of various viruses, such as HIV, HCV, and HPV, in clinical settings to monitor viral loads and assess treatment efficacy.
Nucleic acid amplification techniques (NAATs) are medical laboratory methods used to increase the number of copies of a specific DNA or RNA sequence. These techniques are widely used in molecular biology and diagnostics, including the detection and diagnosis of infectious diseases, genetic disorders, and cancer.
The most commonly used NAAT is the polymerase chain reaction (PCR), which involves repeated cycles of heating and cooling to separate and replicate DNA strands. Other NAATs include loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), and transcription-mediated amplification (TMA).
NAATs offer several advantages over traditional culture methods for detecting pathogens, including faster turnaround times, increased sensitivity and specificity, and the ability to detect viable but non-culturable organisms. However, they also require specialized equipment and trained personnel, and there is a risk of contamination and false positive results if proper precautions are not taken.
"Self-Sustained Sequence Replication" is not a recognized medical term. It appears to be related to the field of molecular biology, specifically in the study of DNA replication and gene expression. However, I am an assistant trained to assist with general knowledge questions and not a medical professional. Therefore, I would recommend consulting a reliable medical source or speaking with a healthcare provider for accurate information regarding this term.
Sensitivity and specificity are statistical measures used to describe the performance of a diagnostic test or screening tool in identifying true positive and true negative results.
* Sensitivity refers to the proportion of people who have a particular condition (true positives) who are correctly identified by the test. It is also known as the "true positive rate" or "recall." A highly sensitive test will identify most or all of the people with the condition, but may also produce more false positives.
* Specificity refers to the proportion of people who do not have a particular condition (true negatives) who are correctly identified by the test. It is also known as the "true negative rate." A highly specific test will identify most or all of the people without the condition, but may also produce more false negatives.
In medical testing, both sensitivity and specificity are important considerations when evaluating a diagnostic test. High sensitivity is desirable for screening tests that aim to identify as many cases of a condition as possible, while high specificity is desirable for confirmatory tests that aim to rule out the condition in people who do not have it.
It's worth noting that sensitivity and specificity are often influenced by factors such as the prevalence of the condition in the population being tested, the threshold used to define a positive result, and the reliability and validity of the test itself. Therefore, it's important to consider these factors when interpreting the results of a diagnostic test.
An encyclopedia is a comprehensive reference work containing articles on various topics, usually arranged in alphabetical order. In the context of medicine, a medical encyclopedia is a collection of articles that provide information about a wide range of medical topics, including diseases and conditions, treatments, tests, procedures, and anatomy and physiology. Medical encyclopedias may be published in print or electronic formats and are often used as a starting point for researching medical topics. They can provide reliable and accurate information on medical subjects, making them useful resources for healthcare professionals, students, and patients alike. Some well-known examples of medical encyclopedias include the Merck Manual and the Stedman's Medical Dictionary.
Nucleic acids are biological macromolecules composed of linear chains of nucleotides. They play crucial roles in the structure and function of cells, serving as the primary information-carrying molecules in all known forms of life. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is responsible for storing genetic information in a stable form that can be passed down from generation to generation, while RNA plays a key role in translating the genetic code stored in DNA into functional proteins.
Each nucleotide consists of a sugar molecule, a phosphate group, and a nitrogenous base. The sugar in DNA is deoxyribose, while in RNA it is ribose. The nitrogenous bases found in both DNA and RNA include adenine (A), guanine (G), and cytosine (C). Thymine (T) is found in DNA, but uracil (U) takes its place in RNA. These nucleotides are linked together by phosphodiester bonds between the sugar of one nucleotide and the phosphate group of another, forming a long, helical structure with backbones made up of alternating sugar and phosphate groups.
The sequence of these nitrogenous bases along the nucleic acid chain encodes genetic information in the form of codons, which are sets of three consecutive bases that specify particular amino acids or signals for protein synthesis. This information is used to direct the synthesis of proteins through a process called transcription (converting DNA to RNA) and translation (converting RNA to protein).
In summary, nucleic acids are essential biomolecules composed of chains of nucleotides that store, transmit, and express genetic information in cells. They consist of two main types: DNA and RNA, which differ in their sugar type, nitrogenous bases, and functions.
Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify specific regions of DNA. It enables the production of thousands to millions of copies of a particular DNA sequence in a rapid and efficient manner, making it an essential tool in various fields such as molecular biology, medical diagnostics, forensic science, and research.
The PCR process involves repeated cycles of heating and cooling to separate the DNA strands, allow primers (short sequences of single-stranded DNA) to attach to the target regions, and extend these primers using an enzyme called Taq polymerase, resulting in the exponential amplification of the desired DNA segment.
In a medical context, PCR is often used for detecting and quantifying specific pathogens (viruses, bacteria, fungi, or parasites) in clinical samples, identifying genetic mutations or polymorphisms associated with diseases, monitoring disease progression, and evaluating treatment effectiveness.
Semen preservation is the process of collecting, liquefying, testing, and storing semen samples for future use in assisted reproductive technologies (ART) such as artificial insemination (AI), in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI). The semen sample is usually collected through masturbation, and then it is mixed with a cryoprotectant solution to prevent damage during the freezing and thawing process. After that, the sample is divided into straws or vials and frozen in liquid nitrogen tanks at temperatures below -196°C. Properly preserved semen can be stored for many years without significant loss of quality or fertility potential. Semen preservation is often recommended for men who are about to undergo medical treatments that may affect their sperm production or fertility, such as chemotherapy or radiation therapy, or for those who wish to postpone fatherhood for personal or medical reasons.
Branched DNA assay
List of MeSH codes (E05)
In situ hybridization
Precision diagnostics
Transfection
Viral load
Synthetic biology
Rolling circle replication
Schistosoma mansoni
TPX2
HIV
Molecular biology
Hydrogen peroxide
Single-cell analysis
List of RNA-Seq bioinformatics tools
Biosearch Technologies
BRCA1
Spinocerebellar ataxia type 1
Herbicide
Glossary of genetics (M-Z)
Genomics
Haplogroup T-M184
Phytoplasma
William N. Rom
MTOR inhibitors
Glossary of genetics (0-L)
Single-cell transcriptomics
Polymerase chain reaction
Microfluidics
Dipshikha Chakravortty
Branched DNA assay - Wikipedia
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Nucleic acid10
- In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. (wikipedia.org)
- The label oligonucleotide and the branched DNA then detects the immobilized target nucleic acid. (wikipedia.org)
- The branched DNA binds to the sample nucleic acid by specific hybridization in areas which are not occupied by capture hybrids. (wikipedia.org)
- The design of the branched DNA and the way it is hybridized to the nucleic acid to be investigated differs between different generations of the bDNA assay. (wikipedia.org)
- A number of nucleic acid-based detection methods can be used to detect low levels of HIV-1 RNA or DNA, including PCR (standard, quantitative competitive, real time, in situ), branched DNA signal amplification (bDNA), strand displacement amplification (SDA), transcription-mediated amplification (TMA), and nucleic acid sequence-based amplification (NASBA). (ucsf.edu)
- Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
- Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
- Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample. (ctsicn.org)
- This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
- We present a new approach for relating nucleic-acid content to fluorescence in a real-time Polymerase Chain Reaction (PCR) assay. (smb.org)
Quantitative5
- The assay can be run as a high throughput assay, unlike quantitative Northern-blotting or the RNAse-protection assay, which are labor-intensive and thus difficult to perform on a large number of samples. (wikipedia.org)
- Quantitative assays for HCV RNA Tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification. (cdc.gov)
- Of the truly quantitative techniques, HIV-1 RNA assays of tissue homogenates held the greatest promise for objective cross-study comparisons. (mahidol.ac.th)
- Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
- HBV DNA (quantitative viral load) indicates viral burden and viral replication. (medscape.com)
Commercial assays1
- DiaCarta has other commercial assays, a line of human papillomavirus tests that use branched DNA technology licensed from Siemens. (genomeweb.com)
Quantification4
- An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma. (ox.ac.uk)
- The branched-DNA (bDNA) assay provides a reliable method for the quantification of HIV-1 RNA in human plasma and is considered one of the most reproducible assays ready for use in clinical trials. (ox.ac.uk)
- Further, quantification values obtained with the ES bDNA assay and the first-generation bDNA assay are highly correlated, thus allowing for meaningful comparisons of HIV-1 RNA levels in specimens tested with either assay. (ox.ac.uk)
- QuantiGene® 2.0 miRNA Assay -based on DIRECT quantification of the miRNA target using branched DNA (bDNA) signal amplification technology and uses a microplate luminometer for the read out. (aykaltd.com)
Oligonucleotide3
- A series of oligonucleotide probe design and solution changes have been developed to enhance the sensitivity of the bDNA assay while maintaining its performance characteristics. (ox.ac.uk)
- The assay combines paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect up to three RNA transcripts at the single-cell level using a standard flow cytometer. (technion.ac.il)
- Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
BDNA assay3
- Among the changes incorporated into the enhanced-sensitivity bDNA (ES bDNA) assay to reduce the background level and enhance the signal are the use of shorter overhang sequences of target probes for capture, the cruciform design of target probes for amplification, and the addition of preamplifier molecules. (ox.ac.uk)
- The ES bDNA assay is at least 20-fold more sensitive than the first-generation bDNA assay, yet it maintains a high level of accuracy, linearity, and reproducibility. (ox.ac.uk)
- The ES bDNA assay may be useful in determining the prognostic value of HIV-1 RNA levels of below 10,000 copies per ml and in assessing the clinical benefit of antiretroviral therapy-induced decreases in plasma HIV-1 RNA sustained at levels of below 10,000 copies per ml. (ox.ac.uk)
Immunoblot assay1
- Recombinant immunoblot assay. (cdc.gov)
Alkaline phosphatase2
- After binding of the target to the solid support it can be detected by branched DNA which is coupled to an enzyme (e.g. alkaline phosphatase). (wikipedia.org)
- Immobilized hybrids are then reacted with alkaline phosphatase conjugated antibodies specific for the RNA: DNA hybrids, and detected with a chemiluminescent substrate. (cdc.gov)
Viral1
- Qualitative RT-PCR for HCV RNA Test to detect HCV RNA by amplification of viral genetic sequences. (cdc.gov)
Molecules8
- The dish is peppered with small, single stranded DNA molecules (or chains) that stick out into the solution. (wikipedia.org)
- These are known as capture probe DNA molecules. (wikipedia.org)
- First, it creates more available surface area for target DNA molecules to bind, and second, it allows the assay to be easily adapted to detect a variety of target DNA molecules. (wikipedia.org)
- Several different short single-stranded DNA molecules (oligonucleotides) are used in a branched DNA-assay. (wikipedia.org)
- Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
- Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)
- RANK lacks intrinsic enzymatic activity in its intracellular domain, and it transduces signaling by recruiting adaptor molecules such as the TRAF family of proteins [8]. (plasignaling.com)
- Human signaling network development To establish a extensive human signaling network, we manually curated the cellular signaling molecules which integrated varied pathways sources together with BioCarta, literature mined network, Cancer Cell Map and HPRD. (fak-signal.com)
Enzyme5
- The branching of the DNA allows for very dense decorating of the DNA with the enzyme, which is important for the high sensitivity of the assay[citation needed]. (wikipedia.org)
- However, in clinical practice, the most common method for diagnosing established HIV infection is by performing a screening test (eg, enzyme-linked immunosorbent assay [ELISA]) and by confirming a positive result with a supplementary test. (medscape.com)
- Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
- It involves randomly nicking the backbone of a double-stranded DNA with dilute concentrations of DNase I. At extremely low concentrations, this enzyme nicks a template at four or five sites, producing a free 3′-OH group that can act as a primer at each nicking location. (enzolifesciences.com)
- According to the prediction of bioinformatics software of Miranda, we showed that 5′-UTR regions of hsa-miR-124-3p, a mature sequence of human miR-124 precursor, could bind to 3′-UTR region of branched chain amino acid transaminase 1(BCAT1) gene, the enzyme that catalyzes branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth [ 15 , 16 ]. (biomedcentral.com)
Pathways3
- Modulates signaling in ERK, SRC and NF-kappa-B pathways. (cusabio.com)
- They are key regulators of brassinosteroid signaling and are also involved in the cross-talk between auxin and brassinosteroid pathways. (biomedcentral.com)
- In humans and animals, GSK-3/SGG are key regulators of a broad range of signaling pathways and their dysregulation responsible for a number of diseases or developmental abnormalities, both aspects abundantly documented in the literature. (biomedcentral.com)
Sequences3
- An RLU measurement equal to or greater than the Cutoff Value indicates the presence of HPV DNA sequences in the specimen. (cdc.gov)
- An RLU measurement less than the Cutoff Value indicates the absence of the specific HPV DNA sequences tested or HPV DNA levels below the detection limit of the assay. (cdc.gov)
- DNA and RNA are composed of linked sequences of nucleotides . (agemed.org)
Vitro2
- To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine-alginate (PEI-al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. (dovepress.com)
- In vitro kinase assays showed that TaSK1 and TaSK2 possessed kinase activity. (biomedcentral.com)
Probe10
- Diagrammatically, the process can be resembled as Base → Capture Probe → Extender → Target → label extender → pre-amplifier → amplifier The assay can be used to detect and quantify many types of RNA or DNA target. (wikipedia.org)
- DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
- While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)
- A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
- The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
- Specimens containing the target DNA hybridize with the HR HPV RNA probe cocktail. (cdc.gov)
- Trigging isothermal circular amplification-based tuning of rigorous fluorescence quenching into complete restoration on a multivalent aptamer probe enables ultrasensitive dete ction of Salmonella. (translationandethics.com)
- 2] Engineered G-quadruplex-embedded self-quenching probes regulate single probe-based multiplex isothermal amplification to light road lamp probes for sensitized determination of microRNAs. (translationandethics.com)
- Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination. (translationandethics.com)
- Delayed full opening of bumped switchable molecular probe enables repeated generation of target analogues for mix-to-signaling determination of microRNAs. (translationandethics.com)
Detection6
- This novel assay employs a proprietary fluorescent in situ hybridization (FISH) technique enabling simultaneous detection of up to three RNA transcripts in a single cell using a standard flow cytometer. (technion.ac.il)
- Detection and typing of HPV DNA in vaginal swabs (in conjunction with testing of NHANES sera for HPV antibody) will allow evaluation of trends in prevalence of type-specific HPV infection by age, sexual behavior, and race/ethnicity. (cdc.gov)
- It uses chemiluminescence for the qualitative detection of human papillomavirus (HPV) DNA in cervical specimens. (cdc.gov)
- After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
- However, product information on Roche's website claims its BRAF test detects mutations at less than a 5 percent mutation level, while DiaCarta claims its XNA method enables detection of less than 0.1 percent mutated DNA. (genomeweb.com)
- Powell added that the company is already able to perform V600E BRAF mutation detection on such a strip, although this version of the assay is not yet commercially available. (genomeweb.com)
Regulate1
- We subsequently use this approach to refine our understanding of pancreatic beta cell signaling dynamics, which regulate beta cell proliferation. (smb.org)
Detect2
- Despite the fact that the starting material is not preamplified, bDNA assays can detect less than 100 copies of HIV-RNA per mL of blood. (wikipedia.org)
- BCL2 antibodies targeted at different epitopes detect varying levels of protein expression and correlate with frequent gene amplification in diffuse large B-cell lymphoma. (uams.edu)
Intracellular1
- Alternative splicing affects both the extracellular ligand-binding domains and the intracellular signal-transducing domains of the trks ( Barbacid, 1994 ). (jneurosci.org)
Binds1
- A veteran of Boehringer Mannheim and Roche Diagnostics, Powell helped develop the XNA method, which binds wild-type DNA "like Velcro," he said. (genomeweb.com)
Amplifies1
- It amplifies the signal (its target) and the noise (anything in the background). (planetwaves.net)
Inhibition1
- The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)
Bind1
- EMSA and dual-luciferase reporter assays further confirmed that STM3 could directly bind the promoter region to activate FUL1 expression. (nature.com)
Fluorescence1
- By coupling a two-type stochastic branching process for PCR with a fluorescence analog of Beer's Law, the approach reduces bias and quantifies uncertainty in fluorescence. (smb.org)
Hybrids1
- The resultant RNA: DNA hybrids are captured onto the surface of a microplate well coated with antibodies specific for RNA: DNA hybrids. (cdc.gov)
Pathway2
- The classical NF-κB MGCD0103 datasheet signaling pathway involves the activation of the IKK complex, which phosphorylates IκBα and targets them for ubiquitin-dependent degradation [8]. (plasignaling.com)
- IKK is the major upstream kinase of IκBα in the NF-κB signaling pathway. (plasignaling.com)
Quantify2
- Quantify DNA, RNA, and protein in seconds using only 1-2 µL of sample-no need for dilution. (thermofisher.cn)
- The Invitrogen Qubit Fluorometer is designed to quickly and specifically quantify DNA or RNA. (thermofisher.cn)
Complementary1
- As the two-type branching process distinguishes between complementary strands of DNA, it allows for a stoichiometric description of reactions between fluorescent probes and DNA and can capture the initial conditions encountered in assays targeting RNA. (smb.org)
Distinct1
- Different tomato varieties require distinct inflorescence-branching structures to enhance productivity. (nature.com)
Probes4
- What are the Differences Between DNA and RNA Probes? (enzolifesciences.com)
- What are DNA probes? (enzolifesciences.com)
- A variety of methodologies for labeling DNA are used to generate end-labeled or continuously labeled probes. (enzolifesciences.com)
- Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Hybridization1
- The assay entirely relies on hybridization. (wikipedia.org)
Diagnostic2
- We have a BRAF assay [for] a melanoma mutation, a resistance mutation" similar to Roche's US Food and Drug Administration-approved Cobas 4800 BRAF V600 companion diagnostic for BRAF mutation-positive metastatic melanoma. (genomeweb.com)
- Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision. (who.int)
Polymerase1
- This assay uses Roche Linear Array HPV Genotyping test that is based on HPV L1 consensus polymerase chain reaction (PCR) with biotinylated PGMY09/11 primer sets. (cdc.gov)
Methylation2
- DNA methylation is one of the best known epigenetic mechanisms in cancer epigenetics. (biomedcentral.com)
- DNA methylation involves in the addition of a methyl (CH3) group to DNA with DNA methyltransferases (DNMTs), thereby often modifying gene function through regulation of gene expression [ 7 ]. (biomedcentral.com)
Dynamics1
- Flow Cytometry RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to stimulus. (technion.ac.il)
Proteins1
- Internal signals producing apoptosis depend on interactions of several proteins and may serve to protect the organism from cancer by killing cells that have pre-cancerous changes. (agemed.org)
Mutation1
- Mutation of FUL1 could partially restore inflorescence-branching phenotypes caused by high STM3 expression in ST024. (nature.com)
Apoptosis3
- Signals to trigger apoptosis may come from within the cell or from outside, by stimulating suicide receptors in the cell's external membrane. (agemed.org)
- The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. (researchsquare.com)
- Ionizing radiation is well known to induce oxidative DNA damage, such as DNA double-strand breaks (DSBs), and consequently trigger the DNA damage response (DDR), including cell cycle arrest and apoptosis. (researchsquare.com)
Involves1
- On the other hand, we clarified the upstream mechanism regulating miR-124-3p expression in ESCC, which involves in the hypermethylation-silencing regulation mediated by DNA methyltransferase 1(DNMT1), which is of high expression in ESCC tissues and cell lines in the present study. (biomedcentral.com)
Gene expression2
- QuantiGene® Plex 2.0 Assays - Multiplex Gene Expression Analysis -provides DIRECT from lysate measurement of 3 to 36 target RNAs per well. (aykaltd.com)
- clinicians require high-resolution data on gene expression, but in standard real-time PCR and sequencing methods, unless a mutated gene makes up at least 10 percent of a sample, its signal is often swamped by super-abundant wild-type DNA. (genomeweb.com)
Genes2
- While a few important genes for tomato inflorescence-branching development have been identified, the regulatory mechanism underlying inflorescence branching is still unclear. (nature.com)
- A series of regulatory genes that have received much attention makes major contributions to inflorescence architecture in tomato by changing the inflorescence-branching pattern. (nature.com)
Cell4
- Using the PrimeFlow™ RNA Assay, specific cell populations may be analyzed for unique transcript expression levels or cell subsets evaluated over time to determine transcriptional regulation and protein expression simultaneously. (technion.ac.il)
- Cell Signal. (jefferson.edu)
- Assays on the cell culture showed that the genetically engineered cells released the BMP-2 for at least 14 days. (dovepress.com)
- The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. (cusabio.com)
Target2
- Favorable inflorescence branching is always a major breeding target for achieving desirable production by balancing the sink-source relationship. (nature.com)
- The intensity of the light emitted denotes the presence or absence of target DNA in the specimen. (cdc.gov)
Capture3
- one that hybridizes to the capture DNA molecule and one that sticks out above the surface. (wikipedia.org)
- Caretti E, Devarajan K, Coudry R, Ross E, Clapper ML, Cooper HS, Bellacosa A. Comparison of RNA amplification methods and chip platforms for microarray analysis of samples processed by laser capture microdissection. (jefferson.edu)
- download by Amazon( FBA) is a event we refer databases that is them be their systems in Amazon's function views, and we badly Sign, Capture, and tie cross assay for these processes. (liebherr-bhb.de)
Primers1
- It also includes biotinylated β-globin primers as an internal control for sample amplification. (cdc.gov)
Development2
- Development of the PrimeFlow™ RNA Assay is based upon proven and well-published ViewRNA™ technology designed for microscopic analysis of RNA in cells and tissues. (technion.ac.il)
- STM3 is expressed in both vegetative and reproductive meristematic tissues and in leaf primordia and leaves, indicative of its function in flowering time and inflorescence-branching development. (nature.com)
High2
- High expression levels of STM3 underlie the highly inflorescence-branching phenotype in ST024. (nature.com)
- As with amplification, turn it up high enough and the signal and the noise blend into one thing. (planetwaves.net)
Platforms1
- The tests, currently for research use only, can be run in under two hours on existing real-time PCR platforms without a DNA extraction step, on a variety of sample types, the company claims. (genomeweb.com)
Samples1
- Our complete range of solutions addresses diverse research needs, from RNA to DNA analysis, a few to thousands of samples, and targeted to genome-wide analysis. (aykaltd.com)
Receptor1
- The trk receptors, a family of receptor tyrosine kinases including trkA, trkB, and trkC, serve as the principal signal-transducing receptors for the neurotrophins ( Barbacid, 1994 ). (jneurosci.org)
Acid1
- DNA Deoxyribonucleic acid. (cdc.gov)
Method2
- Nick Translation DNA Labeling System 2.0 to provide a simple and efficient method for generating labeled DNA. (enzolifesciences.com)
- PCR is an amplification method. (planetwaves.net)
Analysis2
- Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. (uchicago.edu)
- A phylogenetic analysis of land plant GSKs indicated that TaSK1 and TaSK2 belong to clade II of plant GSKs, the Arabidopsis members of which are all involved in Brassinosteroid signaling. (biomedcentral.com)