No data available that match "Blotting, Southern"

*  Overexpression of activin A in stage IV colorectal cancer | Gut
B) Southern blot analysis. Filters were hybridised with α-32P labelled cDNA fragments ( ... Authenticity of the PCR products was confirmed by Southern blotting using α-32P labelled ... Northern blot analysis of PolyA+-RNA. PolyA+-RNA (2 μg/lane) was prepared from the cell ... Northern blot analysis of polyA+-RNA isolated from SW 837, SW 1463, and CaCo2 colon ......
*  Albert Kesselring, horoscope for birth date 20 November 1885, born in...
On 5/07/1945, he surrendered the southern half of the German forces to the Americans. ... His military record was blotted by the massacre in March, 1944 of 335 Italian civilians. ... He surrendered the southern half of the German forces to the Americans in 1945. ......,_Albert?cid=wojfileTihoFO-u1349504413
*  Magnetic Isolation of Genomic DNA from Tissue
Southern blotting, and any enzymatic reaction. ......
*  Association of Leukocyte Telomere Length With Circulating Biomarkers of the...
We performed Southern blot analyses and obtained the mean of the terminal restriction ......
*  British Library EThOS: Investigating the role of Armadillo-related proteins in...
Cloning, sequencing and Southern blotting approaches confirmed that PHYSCODILLO2 was a ......
B) Western bl ot analysis of anti-P1 MAb reactivity with recombinant full-length P1 and ... Peroxidase-labeled MAbs were obtained from Southern Biotech at a concentration of 1 mg/ml ... B) Western blot reactivity of anti-P1 MAbs with recombinant full-length P1, CK1, CK2, and ... Western blot analysis of antiNR21 IgG subclass reactivity in the sera from S. mutans and ......
*  Alumni US | University of Southern California, Greater Los Angeles Area
Information on the University of Southern California - contacts, students, faculty, ... Graduates of University of Southern California - the names, photos, skill, job, location ... Biochemistry, Molecular Biology, Western Blotting, Cell Culture, Cell, PCR, Protein ... University of Southern California 2009 - 2011 University of Southern California August ......
*  Microscope
... hybridizationSomatic cellSomatic cellsSomatic hypermutationSomiteSorusSpermSouthern blot ......
... "have spread across southern Iraq and Baghdad" and observes, "Many Iraqis are outraged at ... YOU'VE GOT A BLOT NOW TOO!' HE SAYS IT'S LIKE HERPES AND IT WON'T GO AWAY! CAN YOU SEE IT ......
*  Altered body iron distribution and microcytosis in mice deficient in iron...
The Western blot and Northern blot signals were quantified and results are presented in a ... Southern Biotechnology, Birmingham, AL). Images were acquired using Axiovision 4.3 ... Western blot and Northern blot signals were quantified and are presented as a histogram ( ... In Western blots, the ferritin L-subunit resolves into 2 bands, the bottom one ......

No data available that match "Blotting, Southern"

(1/10141) Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis.

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval.  (+info)

(2/10141) Human papillomavirus DNA in adenosquamous carcinoma of the lung.

AIM: To investigate the presence of human papillomavirus (HPV) DNA in adenosquamous carcinoma of the lung--which is relatively common in Okinawa but not in mainland Japan--and examine its histological features. METHODS: Of 207 cases where primary lung cancers were surgically removed between January 1995 and June 1997 in Okinawa, 23 were adenosquamous carcinoma. HPV was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for E6 and E7 regions of the HPV genome. PCR products were analysed by Southern blotting. Immunohistochemical determination of high molecular weight cytokeratin (HMC) and involucrin was also carried out. RESULTS: 18 cases were positive for HPV DNA by PCR and NISH. HPV types 6, 11, 16, and 18 were found. Seven cases were dual positive for different types of HPV. Using NISH, HPV was also found in the squamous cell components and in neighbouring enlarged adenocarcinoma cells. The HMC and involucrin were demonstrated immunohistochemically in the same areas. CONCLUSIONS: HPV DNA was found in a high proportion (78.3%) of adenosquamous carcinomas in Okinawa, a region where HPV has previously been shown to be prevalent in squamous cell carcinoma of the lung. The adenocarcinoma cells adjacent to the squamous cell carcinoma component were enlarged and positive for HPV, HMC, and involucrin. This is thought to indicate the transition from adenocarcinoma to squamous cell carcinoma.  (+info)

(3/10141) The role of alternative splicing of the adhesion molecule, CD44, in lymphoid malignancy.

AIM: To investigate the expression of CD44 isoforms containing variant exon 6 (v6) in a well characterised cohort of patients with non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukaemia (CLL), and to correlate this with phenotype and disease course. METHODS: Cryostat sections of OCT embedded diagnostic nodal material from NHL patients and cryopreserved mononuclear preparations from CLL patients were used as sources of RNA. After reverse transcription, PCR was carried out with amplimers positioned at either side of the variant exon insertion site to amplify all possible CD44 isoforms. Those isoforms containing v6 were identified after Southern blotting and hybridisation with a radiolabelled oligonucleotide. RESULTS: Of 32 NHL samples analysed, 16 did not express CD44 isoforms containing v6, six expressed an isoform containing exon v6 alone, and 10 expressed v6 long isoforms which contained exon v6 in addition to other variant exons. These data did not correlate with lymphoma classification, disease staging, or the presence or absence of extranodal disease. However, those patients expressing v6 long CD44 isoforms had a worse overall survival than those that did not. The plateau of the survival curves was 50% compared with 82%. No v6 long isoforms were detected in the 21 CLL samples investigated. CONCLUSIONS: The expression of v6 long CD44 isoforms is associated with aggressive disease in NHL, independent of grade, stage, or presence of extranodal disease.  (+info)

(4/10141) Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates.

BACKGROUND: cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. AIM: To characterise cagA and the cag PAI in Japanese H pylori strains. METHODS: H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from Western countries were also studied. Induction of interleukin-8 secretion from gastric epithelial cells was also investigated. RESULTS: All Japanese strains retained cagA. Fifty nine of 63 (94%) strains had all the cag PAI genes. In the remaining four, cag PAI was partially deleted, lacking cagA transcripts and not producing CagA protein. Details of the PAI of these strains were checked; three lacked cagB to cagQ (cagI) and continuously cagS to cag13 (cagII), and the remaining one lacked cagB to cag8. Western cagA negative strains completely lacked cag PAI including cagA. Nucleotide sequence analysis in one strain in which the cag PAI was partially deleted showed that the partial deletion contained 25 kb of cag PAI and the cagA promoter. Interleukin-8 induction was lower with the cag PAI partial deletion strains than with the intact ones. All Japanese cag PAI deleted strains were derived from patients with non-ulcer dyspepsia, whereas 41 of 59 (70%) CagA-producing strains were from patients with peptic ulcers or gastric cancer (p<0.05). CONCLUSIONS: Most Japanese H pylori strains had the intact cag PAI. However, some lacked most of the cag PAI in spite of the presence of cagA. Thus the presence of the cagA gene is not an invariable marker of cag PAI related virulence in Japanese strains.  (+info)

(5/10141) Comparison of Bombyx mori and Helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects.

The cytoplasmic actin genes BmA3 and BmA4 of Bombyx mori were found clustered in a single genomic clone in the same orientation. As a similar clustering of the two cytoplasmic actin genes Ha3a and Ha3b also occurs in another lepidopteran, Helicoverpa armigera, we analyzed the sequence of the pair of genes from each species. Due to the high conservation of cytoplasmic actins, the coding sequence of the four genes was easily aligned, allowing the detection of similarities in noncoding exon and intron sequences as well as in flanking sequences. All four genes exhibited a conserved intron inserted in codon 117, an original position not encountered in other species. It can thus be postulated that all of these genes derived from a common ancestral gene carrying this intron after a single event of insertion. The comparison of the four genes revealed that the genes of B. mori and H. armigera are related in two different ways: the coding sequence and the intron that interrupts it are more similar between paralogous genes within each species than between orthologous genes of the two species. In contrast, the other (noncoding) regions exhibited the greatest similarity between a gene of one species and a gene of the other species, defining two pairs of orthologous genes, BmA3 and HaA3a on one hand and BmA4 and HaA3b on the other. However, in each species, the very high similarities of the coding sequence and of the single intron that interrupts it strongly suggest that gene conversion events have homogenized this part of the sequence. As the divergence of the B. mori genes was higher than that of the H. armigera genes, we postulated that the gene conversion occurred earlier in the B. mori lineage. This leads us to hypothesize that gene conversion could also be responsible for the original transfer of the common intron to the second gene copy before the divergence of the B. mori and H. armigera lineages.  (+info)

(6/10141) Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana.

We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli. Affinity purified GST-ATMKK3 and GST-ATMKK4 proteins contained phosphorylation activity, which shows that both the ATMKK3 and ATMKK4 genes encode functional protein kinases. Northern blot analysis revealed that the ATMKK3 gene expressed in all the organs. The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.  (+info)

(7/10141) Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV.

The topoisomerase IV subunit A gene, parC homolog, has been cloned and sequenced from Pseudomonas aeruginosa PAO1, with cDNA encoding the N-terminal region of Escherichia coli parC used as a probe. The homolog and its upstream gene were presumed to be parC and parE through sequence homology with the parC and parE genes of other organisms. The deduced amino acid sequence of ParC and ParE showed 33 and 32% identity with that of the P. aeruginosa DNA gyrase subunits, GyrA and GyrB, respectively, and 69 and 75% identity with that of E. coli ParC and ParE, respectively. The putative ParC and ParE proteins were overexpressed and separately purified by use of a fusion system with a maltose-binding protein, and their enzymatic properties were examined. The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity. So, the cloned genes were identified as P. aeruginosa topoisomerase IV genes. The inhibitory effects of quinolones on the activities of topoisomerase IV and DNA gyrase were compared. The 50% inhibitory concentrations of quinolones for the decatenation activity of topoisomerase IV were from five to eight times higher than those for the supercoiling activities of P. aeruginosa DNA gyrase. These results confirmed that topoisomerase IV is less sensitive to fluoroquinolones than is DNA gyrase and may be a secondary target of new quinolones in wild-type P. aeruginosa.  (+info)

(8/10141) Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide.

Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug that requires conversion to the bactericidal compound pyrazinoic acid (POA) by the bacterial pyrazinamidase (PZase) activity of nicotinamidase to show activity against Mycobacterium tuberculosis. Mutations leading to a loss of PZase activity cause PZA resistance in M. tuberculosis. M. kansasii is naturally resistant to PZA and has reduced PZase activity along with an apparently detectable nicotinamidase activity. The role of the reduction in PZase activity in the natural PZA resistance of M. kansasii is unknown. The MICs of PZA and POA for M. kansasii were determined to be 500 and 125 micrograms/ml, respectively. Using [14C]PZA and [14C]nicotinamide, we found that M. kansasii had about 5-fold-less PZase activity and about 25-fold-less nicotinamidase activity than M. tuberculosis. The M. kansasii pncA gene was cloned on a 1.8-kb BamHI DNA fragment, using M. avium pncA probe. Sequence analysis showed that the M. kansasii pncA gene encoded a protein with homology to its counterparts from M. tuberculosis (69.9%), M. avium (65.6%), and Escherichia coli (28.5%). Transformation of naturally PZA-resistant M. bovis BCG with M. kansasii pncA conferred partial PZA susceptibility. Transformation of M. kansasii with M. avium pncA caused functional expression of PZase and high-level susceptibility to PZA, indicating that the natural PZA resistance in M. kansasii results from a reduced PZase activity. Like M. tuberculosis, M. kansasii accumulated POA in the cells at an acidic pH; however, due to its highly active POA efflux pump, the naturally PZA-resistant species M. smegmatis did not. These findings suggest the existence of a weak POA efflux mechanism in M. kansasii.  (+info)

Can you share an authentic southern/soul food cornbread recipe?

I really have an itching to learn how to cook southern food. I'm from the North and don't have a clue. Please give me an authentic recipe for cornbread like the type served at soul food restaurants. Thanks.

There are lots of different recipes, depending on what part of the South your recipe hails from.  In Louisiana, for instance, I think the cornbread is sweeter and in Virginia you might be run out of town if you put sugar in your cornbread.

So, here is a recipe from one of the finest Southern cooks to have ever lived, Edna Lewis.  Edna Lewis' history is as interesting as her food.  If you are truly interested in real Southern cooking, Edna's books are a good place to start but it certainly isn't the end.

2 C. sifted white cornmeal
1/2 tsp salt
1/2 tsp baking soda
2 tsps Royal Baking Powder
3 eggs, beaten
1 Tbs lard
1 Tbs butter
2 Cups sour milk or buttermilk.

Sift the cornmeal, salt, soda and baking powder into a mixing bowl.  Stir in the beaten eggs.  At this point se the baking pan in the oven with the alrd and butter added.  Pour the sour milk into the cornmeal batter and stir well.  Now remove the pan from the oven and tilt it all around to oil the who surface of the pan.  Pour off into the batter what fat remains.   Mix well and pour the batter into the hot pan.  Cornmeal batter must be poured into a sizzling hot pan, other wise it will stick.  Bake at 400 degrees F. for 25 to 30 minutes  Remove and cut into squares.

This is as close to the real thing as you will get.  My grandmother used to chop up bacon and cook it a cast iron skillet (instead of using lard and butter), then pour the excess fat into the batter and then the batter into the pan and proceed.

There is no pan better suited or more authentic to use for this than a cast iron skillet.

How do blotting papers for oily skin work?

I've heard you can get blotting papers for oily skin and I want to know which ones are good and how I use them, do I put make up on then later blot or do I leave my face plain because wouldn't the blotting papers just take all your make up off anyway? thanks.
- by the way, are they good? and do they work?

Blotting papers absorb oil. 
You can put on your makeup. The blotting paper will not take your makeup off. And honestly any blotting paper is good. I use the NYX blotting paper and its like what? $3? And it works just as good as the expensive ones.

What are some real southern food recipes?

I just moved to the south, and I am looking for some recipes of real southern foods. Dishes that have been cooked by many southern generations. Like fired okra, fried pickles, fried green tomatoes, and fired chicken. If anyone has some real southern recipes, I would greatly appreciate them. Thank you so very much.
Wow, there are so many good answers, I couldn't choose a best answer. So I am just going to put it up to vote. Thank you all so very, very much. I can't wait to start cooking!!!

I live in Georgia and these are some of my favorites that are tried and true recipes from my family.
Cube Steak with Onion Gravy:
1 lb. cube steak
1 cup flour
Salt, pepper, garlic powder
2 cans beef broth
1 onion, chopped
1 envelope onion soup mix
1 tbsp. worchestershire sauce
Salt, pepper, 1/2 tsp. thyme

Place flour in a shallow dish and season with salt, pepper, and garlic powder. Coat cube steak with flour well and place in a hot skillet with vegetable oil. Brown cube steaks well on both sides and remove from skillet-- you're not cooking them all the way through now. With drippings/oil from the pan add in enough flour to make a roux, usually 2-3 tbsp. Brown the flour/oil/roux mixture until it turns a deep golden brown over medium low heat, usually takes 10 minutes. Whisk in enough beef broth for a gravy-- this you have to use your judgment on. Keep whisking to remove all lumps. Now add in all seasonings such as salt, pepper, thyme. onion soup mix, worchestershire sauce, and chopped onion and stir to combine. Add in browned cube steak and turn heat to low and simmer covered for one and a half hours. You may need to add more broth during cooking time. Serve over hot buttered rice and enjoy!

Here's my recipe for Macaroni and Cheese- southern style that's baked. Boil one 8 oz box of elbow noodles until done and drain. Meanwhile, mix together one 12 oz can evaporated milk, 1 cup sour cream, salt/pepper/cayenne to taste, 1 egg, and 8-16 oz cheddar cheese. You can add as much cheese as you want but hold out some of it for the top of the mac and cheese. Mix that together and add in your cooked elbow macaroni. Pour into a greased casserole dish, top with remaining cheese, and bake at 350 degrees for 30 mintues. Check the mac and cheese to see if it's done. If it still looks like it has liquid left you can cook it for longer or take it out if you like creamy mac and cheese.

Banana Pudding is a southern staple at all potlucks and family reunions. Here's the recipe I use and everyone loves it.
Banana Pudding:
1 can sweetened condensed milk
1 1/2 cups cold water
1 pkg. 4 serving size vanilla pudding
1 pint heavy whipping cream
3 bananas, sliced
1 box vanilla wafers

Whisk the sweetened condensed milk and water together. Add in the pudding mix, stir and chill in fridge. Meanwhile in a chilled bowl, beat the whipping cream with hand mixer until stiff peaks form. Fold the whipped cream together with the pudding/milk mixture. Layer pudding/cream mixture on bottom of another bowl with layers of bananas and wafers until you have used all the pudding mixture, bananas, and wafers. Chill and serve. 

Buttermilk Biscuits:
2 cups all purpose flour
1 tbsp. baking powder
1/2 tsp. baking soda
1 tsp. salt
1/4 cup shortening, chilled
1/4 cup butter, chilled
3/4 cup buttermilk
Mix together flour, salt, baking powder, and baking soda in a bowl. Cut in shortening and butter until the fats are the size of small peas. Stir in buttermilk gently. If dough is dry add a few more tablespoons of buttermilk. Depending on the weather you may need more or less buttermilk. Turn dough out onto floured surface and roll to thickness desired (1/2 inch for high biscuits). Cut with biscuit cutter and place on greased baking sheet and brush tops of biscuits with extra buttermilk or melted butter. Bake at 500 degrees for 8-10 minutes.

What is the best blotting sheet for oily skin?

I really like blotting papers but I want one that is effective.  I've tried Clean & Clear but it didn't work very well.  I'm considering Palladio sheets which has powder in translucent.  But I'm not sure.  I'm also considering Mary Kay or Pro Activ.  What do you think?
I like to blot because its easy and convenient.  So im asking for blotting sheets

I like Shiseido's blotting papers.  They have a small amount of powder on one side to help diminish shininess as you blot (Even after I blot, I'm shiny).  $10 for around 100 sheets, and refills I think are 5.  

I trust Shiseido because they're a skincare company that has been in business for almost 150 years... if you've been in business that long, you have to know what you're talking about!

What is cheaper? a bottle of southern comfort or a bottle jack daniels? What are the prices of each?

What is cheaper? a bottle of southern comfort or a bottle jack daniels? What are the prices of each?
which tastes better? and which has more proof?

SOUTHERN COMFORT 35% abv £16.99
JACK DANIELS  43% abv £20.99

What is cheaper? a bottle of southern comfort or a bottle jack daniels? What are the prices of each?

What is cheaper? a bottle of southern comfort or a bottle jack daniels? What are the prices of each?
which tastes better? and which has more proof?

Generally locally Southern Comfort is Cheaper

What are the best recipes for southern cooking?

You see , I want to cook southern meals because everyone is always saying how wonderfully delicious they are! I just don't know which recipes are actually southern food. I was thinking of moving down there, but I think that may be too drastic...
loads of yummy southern recipes

Where can I find some real southern comfort food recipes?

My mother in law makes the best southern cooking and I miss it so much! Where can I find recipes to make some authentic southern comfort food?
---------- is a great site- you can find just about any recipe you want with a southern flair. Start  with sweet tea, corn bread, and this way over the top macaroni and cheese, from a very southern "belle"

Over-the-Rainbow Macaroni and Cheese
Recipe created by Patti LaBelle

Patti LaBelle says that the sure sign of a good cook is if their macaroni and cheese will make you cry! According to Oprah, Patti's version just might bring tears to your eyes. 

 1 tablespoon vegetable oil 
 1 pound elbow macaroni  
 8 tablespoons (1 stick) plus 1 tablespoon butter 
 1/2 cup (2 ounces) shredded Muenster cheese 
 1/2 cup (2 ounces) shredded mild Cheddar cheese  
 1/2 cup (2 ounces) shredded sharp Cheddar cheese 
 1/2 cup (2 ounces) shredded Monterey Jack 
 2 cups half-and-half  
 1 cup (8 ounces) Velveeta, cut into small cubes 
 2 large eggs, lightly beaten 
 1/4 teaspoon seasoned salt 
 1/8 teaspoon freshly ground black pepper  

Preheat the oven to 350 degrees F. Lightly butter a deep 2 1/2-quart casserole. 

Bring the large pot of salted water to a boil over high heat. Add the oil, then the elbow macaroni, and cook until the macaroni is just tender, about 7 minutes. Do not overcook. Drain well. Return to the cooking pot. 

In a small saucepan, melt eight tablespoons of the butter. Stir into the macaroni. In a large bowl, mix the Muenster, mild and sharp Cheddar, and Monterey Jack cheeses. To the macaroni, add the half-and-half, 1 1/2 cups of the shredded cheese, the cubed Velveeta, and the eggs. Season with salt and pepper. Transfer to the buttered casserole. Sprinkle with the remaining 1/2 cup of shredded cheese and dot with the remaining one tablespoon of butter. 

Bake until it's bubbling around the edges, about 35 minutes. Serve hot.