Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Kinetics: The rate dynamics in chemical or physical systems.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Ligands: A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Binding Sites, Antibody: Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Consensus Sequence: A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Sp1 Transcription Factor: Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.DNA Footprinting: A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Radioligand Assay: Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Bacterial Proteins: Proteins found in any species of bacterium.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Transcriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Affinity Labels: Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Chromatin Immunoprecipitation: A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Deoxyribonuclease I: An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Electrophoretic Mobility Shift Assay: An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Regulatory Elements, Transcriptional: Nucleotide sequences of a gene that are involved in the regulation of GENETIC TRANSCRIPTION.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Autoradiography: The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)Oligodeoxyribonucleotides: A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Deletion: Deletion of sequences of nucleic acids from the genetic material of an individual.Spectrometry, Fluorescence: Measurement of the intensity and quality of fluorescence.Dimerization: The process by which two molecules of the same chemical composition form a condensation product or polymer.Allosteric Regulation: The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Iodine Radioisotopes: Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Response Elements: Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Allosteric Site: A site on an enzyme which upon binding of a modulator, causes the enzyme to undergo a conformational change that may alter its catalytic or binding properties.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Nucleotide Motifs: Commonly observed BASE SEQUENCE or nucleotide structural components which can be represented by a CONSENSUS SEQUENCE or a SEQUENCE LOGO.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Thermodynamics: A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)Photoaffinity Labels: Biologically active molecules which are covalently bound to the enzymes or binding proteins normally acting on them. Binding occurs due to activation of the label by ultraviolet light. These labels are used primarily to identify binding sites on proteins.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Cross-Linking Reagents: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Molecular Weight: The sum of the weight of all the atoms in a molecule.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Receptors, Nicotinic: One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.Zinc: A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Homeodomain Proteins: Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Luciferases: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.TritiumCell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Molecular Docking Simulation: A computer simulation technique that is used to model the interaction between two molecules. Typically the docking simulation measures the interactions of a small molecule or ligand with a part of a larger molecule such as a protein.Surface Plasmon Resonance: A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Azides: Organic or inorganic compounds that contain the -N3 group.COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Transcription Initiation Site: The first nucleotide of a transcribed DNA sequence where RNA polymerase (DNA-DIRECTED RNA POLYMERASE) begins synthesizing the RNA transcript.Cations, Divalent: Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.NFI Transcription Factors: Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.Molecular Conformation: The characteristic three-dimensional shape of a molecule.Circular Dichroism: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Chloramphenicol O-Acetyltransferase: An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.Receptors, Neurotransmitter: Cell surface receptors that bind signalling molecules released by neurons and convert these signals into intracellular changes influencing the behavior of cells. Neurotransmitter is used here in its most general sense, including not only messengers that act to regulate ion channels, but also those which act on second messenger systems and those which may act at a distance from their release sites. Included are receptors for neuromodulators, neuroregulators, neuromediators, and neurohumors, whether or not located at synapses.Receptors, Drug: Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified.Zinc Fingers: Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.YY1 Transcription Factor: A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Xenopus laevis: The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Hydrogen Bonding: A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.Structural Homology, Protein: The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Membranes: Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.Epitopes: Sites on an antigen that interact with specific antibodies.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)CCAAT-Enhancer-Binding Proteins: A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Catalytic Domain: The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.TATA Box: A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Cysteine: A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.Nuclear Magnetic Resonance, Biomolecular: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.Protein Structure, Quaternary: The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Proto-Oncogene Proteins c-ets: A family of transcription factors that share a unique DNA-binding domain. The name derives from viral oncogene-derived protein oncogene protein v-ets of the AVIAN ERYTHROBLASTOSIS VIRUS.Lysine: An essential amino acid. It is often added to animal feed.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Introns: Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Protein Subunits: Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.Calorimetry: The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Tryptophan: An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.PhotochemistryTorpedo: A genus of the Torpedinidae family consisting of several species. Members of this family have powerful electric organs and are commonly called electric rays.Nucleotides: The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Time Factors: Elements of limited time intervals, contributing to particular results or situations.Bungarotoxins: Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.Protein Interaction Domains and Motifs: Protein modules with conserved ligand-binding surfaces which mediate specific interaction functions in SIGNAL TRANSDUCTION PATHWAYS and the specific BINDING SITES of their cognate protein LIGANDS.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Protein Interaction Mapping: Methods for determining interaction between PROTEINS.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Cations: Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.Terbium: Terbium. An element of the rare earth family of metals. It has the atomic symbol Tb, atomic number 65, and atomic weight 158.92.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Oligopeptides: Peptides composed of between two and twelve amino acids.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.5' Flanking Region: The region of DNA which borders the 5' end of a transcription unit and where a variety of regulatory sequences are located.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Fungal Proteins: Proteins found in any species of fungus.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Position-Specific Scoring Matrices: Tabular numerical representations of sequence motifs displaying their variability as likelihood values for each possible residue at each position in a sequence. Position-specific scoring matrices (PSSMs) are calculated from position frequency matrices.Integration Host Factors: Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.Transcription Factor AP-2: A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).HEK293 Cells: A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.Gene Expression Regulation, Viral: Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.3' Untranslated Regions: The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Muscles: Contractile tissue that produces movement in animals.Exons: The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.

*  3H]acetylcholine binding sites in brain. Effect of disulfide bond modification....
... acetylcholine binding sites while the affinity of these sites was unaltered. Reoxidation ... 3H]acetylcholine binding sites in brain. Effect of disulfide bond modification.. R D ... 3H]acetylcholine binding sites in brain. Effect of disulfide bond modification.. R D ... 3H]acetylcholine binding sites in brain. Effect of disulfide bond modification.. R D ......
http://molpharm.aspetjournals.org/content/24/3/387
*  The 5-Hydroxytryptamine6 Receptor-Selective radioligand [3H]Ro 63-0563 Labels 5...
Equilibrium binding of [3H]Ro 63-0563 to 5-HT6 receptor-like binding sites in rat ... Equilibrium binding of [3H]Ro 63-0563 to 5-HT6 receptor-like binding sites in porcine ... 3H]Ro 63-0563 binding to 5-HT6receptor-like binding sites in rat striatum.. In ... 3H]Ro 63-0563 binding to 5-HT6receptor-like binding sites in porcine striatum.. Specific ......
http://molpharm.aspetjournals.org/content/54/3/577
*  Human acetylcholinesterase binds to mouse laminin-1 and human collagen iv by an...
... and human collagen iv by an electrostatic mechanism at the peripheral anionic site.. ......
http://scholar.sun.ac.za/handle/10019.1/76888
*  SharePoint 2013 on-site training and workshop for The Binding Site
Binding Site received their SharePoint 2013 onsite training session and sign-off of their ... SharePoint 2013 on-site training and workshop for The Binding Site Friday, June 20, 2014 ... Binding Site received their SharePoint 2013 onsite training session and sign-off of their ... The training day was a complete success and The Binding Site is planning to Go-Live at ......
http://psp-it.co.uk/about-us/news/SharePoint-2013-on-site-training-and-workshop-for-The-Binding-Site/
*  Iran Nuclear Deal Not 'Legally Binding,' White House Admits
FROM THE HOMEPAGE. *. EXCLUSIVE - Terrorism Expert: H.R. McMaster is Endangering U.S. ... "The success of the JCPOA will depend not on whether it is legally binding or signed, but ... "legally binding" basis. The deal, paraded by President Obama as a surefire measure to ... Establishment Mouthpiece Perino Implies Pro-Israel Breitbart a Nazi Site0 comments - 21 ......
http://breitbart.com/national-security/2015/11/25/iran-nuclear-deal-not-legally-binding-obama-administration-admits/
*  Where's the New York bound 117? - Newark njtransit newjersey | Ask MetaFilter
Ask MetaFilter is a question and answer site that covers nearly any question on earth, ... Where's the New York bound 117?. April 27, 2006 4:53 AM Subscribe. Where do I catch the ... Has anyone ever done this before? Where do I go to catch the New York bound Local 117 ... Wait for the New York bound 107 Local (good idea to pick up a schedule when you leave ......
http://ask.metafilter.com/37117/Wheres-the-New-York-bound-117
*  Molecular analysis of the interaction of LCMV with its cellular receptor α...
... the low affinity binding variant ARM53b was displaced from its receptor binding site by ... The binding site of LCMV on α-DG overlaps with the one used by laminin-1 and likely other ... The binding sites for LCMV and laminin-1 on α-DG overlap. Based on their ubiquitous ... Amino acids 169-408 of α-DG contain an essential virus binding site. Based on previous ......
http://jcb.rupress.org/content/155/2/301
*  Kamran, Shehzad back in West Indies-bound Pakistan squad | Business Standard...
Shehzad back in West Indies-bound Pakistan squad on Business Standard. The Inzamam ul Haq ... Home*Today's Paper*Latest News*Economy*Finance*Current Affairs*International*Management* ... Kamran, Shehzad back in West Indies-bound Pakistan squad IANS , Lahore. March 15, 2017 ......
http://business-standard.com/article/news-ians/kamran-shehzad-back-in-west-indies-bound-pakistan-squad-117031500866_1.html
*  Properties of a LacY efflux mutant
B) Co-substrate binding sites. The N- or C-terminal six helices are colored as gray ... ... A mechanism has been proposed for LacY in which both sugar- and H+- binding sites become ... 1A). The sugar-binding site is located primarily in the N-terminal bundle on helices IV ( ... and site-directed thiol crosslinking (16) indicate that sugar binding or to a lesser ......
http://pubmedcentralcanada.ca/pmcc/articles/PMC2754601/?lang=en-ca
*  Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7...
Atg7-binding unmasks conserved E2 active site architectures For both Atg3 and Atg10, the ... The other Atg7 retains the E2-binding NTD, but carries an active site cysteine-to-alanine ... Despite overlapping binding surfaces, the distinct E2 binding modes suggest that ... Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a non- ......
http://biomedsearch.com/nih/Noncanonical-E2-recruitment-by-autophagy/23142976.html
*  DNA binding site
... s are often associated with specialized proteins known as transcription factors, and are thus linked to transcriptional regulation. DNA binding sites also encompasses the targets of other proteins, like restriction enzymes, site-specific recombinases see site-specific recombination and methyltransferase s. 2 Types of DNA binding sites History and main experimental techniques Databases Representation of DNA binding sites Computational search and discovery of binding sites See also References Further reading. Thus, we can distinguish between transcription factor-binding sites, restriction sites and recombination sites. This makes it difficult to accurately represent transcription factor binding sites using consensus sequences, and they are typically represented using position specific frequency matrices PSFM, which are often graphically depicted using sequence logo s. From then on, DNA binding sites for many transcription factors, restriction enzymes and site-specific recombinases have been discovered using...
https://en.wikipedia.org/wiki/DNA_binding_site
*  Binding site
... in biochemistry a binding site is a region on a protein or piece of dna or rna to which ligand s specific molecule s and or ion s may form a chemical bond an equilibrium exists between unbound ligands and bound ligands the term saturation refers to the fraction of total binding sites that are occupied at any given time when more than one type of ligand can bind to a binding site competition ensues binding sites also exhibit chemical specificity a measure of the types of ligands that will bond and affinity which is a measure of the strength of the chemical bond binding sites are often an important component of the functional characterization of biomolecule s for example the characterization of the binding site of a substrate to an enzyme is essential to model the reaction mechanism responsible for the chemical change from substrate to product binding sites on proteins can sometimes recognize other proteins when a binding site of one protein identifies with another protein s surface a non covalent bond is ...
https://en.wikipedia.org/wiki/Binding_site
*  Evolution of binding sites for Zinc and Calcium ions playing structural roles - BIROn - Birkbeck I
... nstitutional Research Online. BIROn - Birkbeck Institutional Research Online. Evolution of binding sites for Zinc and Calcium ions playing structural roles. 2008 Evolution of binding sites for Zinc and Calcium ions playing structural roles. The geometry of metal coordination by proteins is well understood, but the evolution of metal binding sites has been less studied. Here we present a study on a small number of well-documented structural calcium and zinc binding sites, concerning how the geometry diverges between relatives, how often nonrelatives converge towards the same structure, and how often these metal binding sites are lost in the course of evolution. Both calcium and zinc binding site structure is observed to be conserved; structural differences between those atoms directly involved in metal binding in related proteins are typically less than 0.5 Å root mean square deviation, even in distant relatives. This allowed us to identify six archetypal metal binding site structures: two archetypal zinc ...
http://eprints.bbk.ac.uk/1104/
*  Adenosine Monophosphate(CID 6083) - Compound BioActivity Data
BioAssay Types: Literature 198. Outcome Active. AID 160455. BioAssay type Literature. PubMed 12459011. Data Table. Outcome Active. AID 162706. BioAssay type Literature. PubMed 12459011. Data Table. 3 Structure. SID 103201463. Outcome Active. AID 358183. BioAssay type Literature. PubMed 1479375. Data Table. IC50 0.14 Inhibition of FBPase in human liver using fructose-2,6phosphate as a substrate Fructose-1,6-bisphosphatase 1 View X Row No. 4 Structure. SID 103201463. Outcome Active. AID 453457. BioAssay type Literature. PubMed 19969452. Data Table. 5 Structure. SID 103201463. Outcome Active. IC50 0.44 Inhibition of FBPase in human liver Fructose-1,6-bisphosphatase 1 View X Row No. IC50 1 Inhibition of human liver FBPase expressed in Escherichia coli by spectrophotometry Fructose-1,6-bisphosphatase 1 View X Row No. Kd 3 Experimentally measured binding affinity data Kd for protein-ligand complexes derived from PDB Chain A, Structural Mechanism For Glycogen Phosphorylase Control By Phosphorylation And Amp View X R...
http://pubchem.ncbi.nlm.nih.gov/assay/assay.cgi?cid=6083
*  Molecular recognition
1 thumbnail|250px|Crystal structure of two isophthalic acids bound to a host molecule through hydrogen bonds. 2 thumbnail|250px|Static recognition between a single guest and a single host binding site. In dynamic recognition binding the first guest at the first binding site induces a conformation change that affects the association constant of the second guest at the second binding site. The term 'molecular recognition' refers to the specific interaction between two or more molecule s through noncovalent bonding such as hydrogen bond ing, metal coordination, hydrophobic forces, "The Binding of Benzoarylsulfonamide Ligands to Human Carbonic Anhydrase is Insensitive to Formal Fluorination of the Ligand" Angew. Biological systems Supramolecular systems Static vs. Molecular recognition plays an important role in biological systems and is observed in between receptor-ligand, "The Binding of Benzoarylsulfonamide Ligands to Human Carbonic Anhydrase is Insensitive to Formal Fluorination of the Ligand" Angew. To achie...
https://en.wikipedia.org/wiki/Molecular_recognition
*  Crystal Structure of a Protein Kinase A Complex
... The structure of the complex clarifies the mechanism for PKA inhibition, and its comparison with the structure of cAMP bound to the regulatory subunit hints at how cAMP binding drives its activation. In the complex, the inhibitor/linker region red of the R subunit blocks the active site of the C subunit between the two lobes. The inhibitor docks to the active site cleft of the C subunit in the absence of cAMP. The linker segment complements site 3, the PBC complements site 2, and the inhibitor site complements site 1 of the C subunit. The binding surface extends from the inhibitor binding site at the active site cleft site 1, across the G helix site 2 and through to the activation loop site 3. Three general features describe the binding: the inhibitor sequence docks to the active site cleft; the linker segment that connects the inhibitor peptide to CBD-A becomes ordered; and the helical subdomain within CBD-A docks onto the large lobe of the C subunit. The inhibitor peptide and linker region are disorder...
http://www-als.lbl.gov/index.php/user-information/user-services/272-crystal-structure-of-a-protein-kinase-a-complex.html
*  SMALL MOLECULES AND PROTEIN ANALYSIS DEVICES BASED ON MOLECULAR IMPRINTED POLYMERS - Patent appl
5 The device of claim 4, wherein a releasable first binding agent:analyte conjugate is bound to said molecular imprinted polymer, wherein an affinity of the analyte for said binding sites of said molecular imprinted polymer is at least equal to an affinity of said first binding agent:analyte conjugate for said analyte-specific binding sites of said molecular imprinted polymer, wherein upon contacting said molecular imprinted polymer with the analyte in the liquid sample, the analyte is bound and said first binding agent:analyte conjugate is displaced, said device further comprising: i a reporter-conjugate binding zone, comprising a reporter-conjugate binding element fixed to said solid support on said flow path of the liquid sample, said reporter-conjugate binding element having binding sites with a detectable, releasable, second binding agent:reporter conjugate attached thereto, wherein an affinity of said first binding agent:analyte conjugate for said analyte-specific binding sites of said reporter conjugat...
http://faqs.org/patents/app/20130102063
*  Active site
The active site consists of residues that form temporary bonds with the substrate binding site and residues that catalyse a reaction of that substrate catalytic site. 2 Binding site Lock and key hypothesis. Induced fit hypothesis. Catalytic site Cofactors Inhibitors Active sites in drug discovery Allosteric sites See also Further reading References. An active site contains a binding site that binds the substrate and orients it for catalysis. There are two proposed models of how enzymes fit to their specific substrate: the lock and key model and the induced fit model. Lock and key hypothesis. Emil Fischer 's lock and key model assumes that the active site is a perfect fit for a specific substrate and that once the substrate binds to the enzyme no further modification occurs. 5 Induced fit hypothesis. The induced fit model is a development of the lock-and-key model and assumes that an active site is flexible and it changes shape until the substrate is completely bound. Reversible inhibitors can be competitive o...
https://en.wikipedia.org/wiki/Active_site
*  Cooperativity
'Cooperativity' is a phenomenon displayed by systems involving identical or near-identical elements, which act non-independently of each other, relative to a hypothetical standard non-interacting system in which the individual elements are acting independently. One manifestation of this is enzyme s or receptor s that have multiple binding site s where the affinity of the binding sites for a ligand is 'apparently' increased, positive cooperativity, or decreased, negative cooperativity, upon the binding of a ligand to a binding site. oxygen is more likely to bind to a hemoglobin bound to one oxygen than to an unbound hemoglobin. A much more general and useful definition of positive cooperativity is: A process involving multiple identical incremental steps, in which intermediate states are statistically 'underrepresented' relative to a hypothetical standard system null hypothesis where the steps occur independently of each other. Likewise, a definition of negative cooperativity would be a process involving multi...
https://en.wikipedia.org/wiki/Cooperativity
*  Uncovering the genome's regulatory code
September 10, 2012 Uncovering the genome's regulatory code September 10, 2012. By mapping a large number of regulatory factors, the team succeeded in revealing an overall plan for gene regulation as well as the intimate details of the mechanisms involved in the immune response. With the new method, just a handful of researchers were able to conduct a study on a similar scale to the mega-team ones, and in a fraction of the time." Their highly efficient, automated method enabled Amit and his team to measure a large number of regulatory proteins and their binding sites in parallel. They exposed immune cells to bacteria – setting the stage for gene activation – and then traced the actions of several dozen different regulatory proteins known to play a role in the immune response over four points in time. Not only were the researchers able to identify the binding locations of each and the genes they activate, but the levels of activation and the mechanisms employed. Related Stories New insight into immune tolerance...
http://medicalxpress.com/news/2012-09-uncovering-genome-regulatory-code.html
*  Panasonic 14mm F2.5 review data at various sites.: Micro Four Thirds Talk Forum: Digital Photography
... Review. Reviews. Sample Images. Videos. Lenses. Forums. Forum index. Micro Four Thirds Talk Change forum Panasonic 14mm F2.5 review data at various sites. Shop cameras lenses ▾. Forum Parent First Previous Next Next unread. jericho77. Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Reply to thread Reply with quote. Forum Parent First Previous Next Next unread. Posted by When Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 3. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Aug 5, 2013. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 1. Re: Panasonic 14mm F2.5 review data at various sites. Alumna Gorp. Aug 5, 2013. ...
http://dpreview.com/forums/post/51930238
*  The genetic basis of a plant-insect coevolutionary key innovation. Proc Natl Acad Sci USA (PDF Downl
Proc Natl Acad Sci USA PDF Download Available. Proc Natl Acad Sci USA. © 2007 by The National Academy of Sciences of the USA www.pnas.org?cgi?doi?10.1073?pnas.0706229104PNAS. fossil calibrated, Bayesian relaxed molecular clock estimation of the Pierinae–Coliadinae divergence. Fossil and molecular data agree that the Brassicales appeared by 90 to 85 Mya, which is much earlier than the parallel evolution of glucosinolates in the Euphorbiaceae 58 Mya 28. In this article, we directly address the timing of the appearance of the glucosinolate-feeding Pierinae, using several independent molecular datasets and various calibration methods to generate a robust estimate of when Pieridae evolved relative to their Brassicales host plants. Pieridae-specific temporal reconstruction used a Bayesian relaxed molecular clock method on EF-1. Current and expected diversity, and divergence estimates for the Coliadinae and Pierinae subfamilies of Pieridae. However, there are significantly more species of Pierinae than expected, eve...
http://researchgate.net/publication/5771953_The_genetic_basis_of_a_plant-insect_coevolutionary_key_innovation._Proc_Natl_Acad_Sci_USA
*  Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protecti
... ve biofilms -- ScienceDaily. Your source for the latest research news. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms. Date: April 26, 2012 Source: The Scripps Research Institute Summary: Scientists have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Scientists at The Scripps Research Institute have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Past research had also revealed that nitric oxide is involved in influencing bacterial biofilm formation. Nitric Oxide Modulates Bacterial Biofilm Formation through a Multicomponent Cyclic-di-GMP Signaling Network. The Scripps Research Institute. "Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms." ScienceDaily. The Scripps Research Institute. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biof...
http://sciencedaily.com/releases/2012/04/120426135128.htm
*  Mankin Lab - Publications
Browse by year: 2014    2013    2012    2011    2010    2009    2008    2007    2006    2005    2004    2003    2002    2001    2000    1999    1998    1997    1996    1995   . Proc Natl Acad Sci USA 111, 9804-9809. 2014 The general mode of translation inhibition by macrolide antibiotics Proc Natl Acad Sci USA 111, 15958–15963. Top. Top. Top. Proc Natl Acad Sci USA 108, 5931-5932. Proc Natl Acad Sci USA 108, 10496-10501. Vázquez-Laslop, N., Ramu, H., Mankin, A. 2011 Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330. Top. Proc Natl Acad Sci USA 107, 1983-1988. A., Xiong, L., Mankin, A. H D 2010 Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107, 17152-...
http://mankinlab.cpb.uic.edu/cgi-bin/publication.cgi
*  GO:1900911 regulation of olefin biosynthetic process
... Services. Research. Training. Industry. About us. QuickGO A fast browser for Gene Ontology terms and annotations. EBI Databases QuickGO GO:1900911 regulation of olefin biosynthetic process. Search for terms by keyword or ID: apoptosis GO:0006915 Search for proteins by name or accession: tropomyosin P06727. Web Services. Dataset. Term Basket. Term Information ID GO:1900911. Name regulation of olefin biosynthetic process. Ontology Biological Process. Definition Any process that modulates the frequency, rate or extent of olefin biosynthetic process. GONUTS GO:1900911 Wiki Page. Synonyms. Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope. Click on the icon for more details. Type Synonym. exact regulation of olefin synthesis. exact regulation of olefin biosynthesis. exact regulation of olefin formation. exact regulation of olefin anabolism. Ancestor Chart This chart is interactive; ...
http://ebi.ac.uk/QuickGO/GTerm?id=GO:1900911
*  Albums by Smokey Robinson : Rhapsody
... JavaScript is disabled in your browser settings. rhapsody.com requires JavaScript. Cancel. Free trial. x Music. Apps Devices. Pricing. Sign up. Company Info. Careers. Press Media. Partners. Account. Customer Support. Redeem Coupon. Buy a Gift. 2015 Rhapsody International Inc. ×. Rhapsody App for. Rhapsody International, Inc. Get app. Have the app. Music Apps Devices Pricing Listen Now Try Rhapsody. Sign In. Home. / Music. / Soul/R B. / Motown. / Smokey Robinson. / Facebook. Twitter. Albums by Smokey Robinson. Main Releases. Play. Smokey & Friends. Smokey Robinson. Play. Be Near Me. Smokey Robinson. Play. The Motown Years Live. Smokey Robinson. Play. Ev'ry Man Should Know. Smokey Robinson. Play. The Solo Anthology. Smokey Robinson. Play. The Stripped Mixes. Smokey Robinson. Play. Time Flies When You're Having Fun. Smokey Robinson. Play. Love Songs. Smokey Robinson. Play. Timeless Love. Smokey Robinson. Play. The Definitive Collection. Smokey Robinson. Play. 20th Century Masters: The Millennium Collection....
http://rhapsody.com/artist/smokey-robinson/albums
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/451.aspx?email=1
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/fuchs_appointed_as_general_counsel_16.aspx
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/robinson_memorial_presents_poster_at_the_apic_annu_494.aspx?email=1
*  Robinson Health Center at Aurora | Robinson Memorial Hospital
robinson health center at aurora robinson memorial hospital about us foundation volunteer speaker s bureau press room contact us patients visitors medical services nursing find a doctor locations careers patient portal home locations robinson health center at aurora robinson health center at aurora walden place aurora ohio the robinson health center at aurora offers expert physicians personalized care and the latest in medical technology in one convenient location services and physician practices aurora primary care diagnostic x ray and laboratory services northeast ohio ob gyn robinson rehab center and sport clinic directions view larger map your name your email recipient email your comments word verification visitors patient portal key phone numbers visiting hours wheelchair assistance wi fi at robinson memorial hospital locations robinson memorial hospital robinson medical arts building robinson professional center robinson health center at aurora robinson health center at brimfield robinson health center ...
http://robinsonmemorial.org/Main/Aurora.aspx?email=1
*  Home | Robinson Memorial Hospital
Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Classes Events. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 2:00 PM - 4:00 PM. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 6:30 PM - 8:30 PM. Ostomy Support Group. Mon, Oct 5, 2015. 7:00 PM - 9:00 PM. Diabetes Support Group. Tue, Oct 6, 2015. 5:00 PM - 6:00 PM. Tue, Oct 6, 2015. 5:00 PM - 6:30 PM. Robinson In The News Robinson Memorial Hospital is now UH Portage Medical Center. Robinson Health System becomes part of University Hospitals on June 1. Robinson Memorial hosts 21st Annual Nursing Research Day. Robinson Memorial Hospital Foundation Hosts 2015 Robinson Society and Heritage Society Distinguished Giving Recognition. Doctors Receiving Top Scores in Patient Satisfaction Congratulations to the following doctors who rank in the 90th percentile on the Press Ganey Patient Satisfaction Survey. Jessica ...
http://robinsonmemorial.org/Main/Home.aspx
*  Rehabilitation | Robinson Memorial Hospital
Rehabilitation. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Locations. Patient Portal. Medical Services. Rehabilitation. Rehabilitation. The Robinson Rehab Center and Sport Clinic works to rehabilitate each patient to his or her maximum potential and works to provide the patient with the necessary skills to maintain his or her progress. Ongoing communication between the patient, therapist and referring physician is the foundation of the center's rehabilitation programs. View Aquatic Classes. Team of Professionals Each therapist strives to stay on top of the latest technology and treatments and passes this knowledge along to patients. All Robinson Rehab Center and Sport Clinic professionals are licensed or certified by the State of Ohio. The center provides:. Aquatic aerobics, aquatic exercise and water arthritis classes in Ravenna. Locations: Robinson Medical Arts Building. 6847 North Chestnut Street Ravenna, Ohio 44266...
http://robinsonmemorial.org/main/rehabilitation.aspx
*  Shamim Patel - UK address and phone number - 192.com
... People. All People Businesses Places Electoral Roll Directors. Make sure the biggest decision you'll make is also the best. People. AZ-People. Surname - Patel. Your results for Shamim Patel. Filter Search. Search by name and/or location using the search form at the top of the page. Get access to likely ages Find old friends and family Double check names of spouses and children Trace your family tree Find out who your neighbours are Verify people are who they say they are. Make sure the biggest decision you'll make is also the best. Find out more. previous. 110 Results for Shamim Patel. Looking for Shamim Patel. Below are the results from the UK Electoral Roll and Company Director data. You can narrow your search by adding a location above if you wish. Name Address Other Occupants Electoral Roll Director Info Length of Occupancy Neighbours Property Price. 1 Shamim Patel. Age Guide: 40-44. Reading, Berkshire, RG2 Full Address. View. 2 Shamim R Patel. Age Guide: 30-34. Leicester, Leicestershire, LE5 Full Ad...
http://192.com/atoz/people/patel/shamim/
*  Phillip Phillips News, Pictures, and Videos | TMZ.com
Home Celebs Phillip Phillips Phillip Phillips. Idol Managers Claim John Mayer's ex-manager plotted to jack "American Idol" champ Phillip Phillips out from under his contract with the show -- according to a new lawsuit, but the bigger news is Phil is a READ MORE > - 9/21/2015 2:51 PM PDT. Phillip Phillips I Want to be Gone, Gone, Gone From My Idol Contract Phillip Phillips says "American Idol" producers are ruining his career ... American Idol Winner Phillip Phillips Mother Popped for DUI Phillip Phillips' mother Sheryl Phillips -- who appeared on several episodes of "American Idol" during Phillip's championship run last season -- was arrested for DUI earlier today, TMZ has READ MORE > - 964 days ago. Phillip Phillips I LOVE MY FAMILY But ... READ MORE > - 1064 days ago. Phillip Phillips Family Idol Champ s Forcing Us To Sell Pawn Shop Phillip Phillips' family says the singer left them high and dry after winning "American Idol" -- refusing to help them out financially -- and now, they've been forced to sell th...
http://tmz.com/person/phillip-phillips/?adid=cr_featured_saga_13
*  Shadowing | Robinson Memorial Hospital
Shadowing. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Home. Volunteer. Shadowing. Shadowing. Robinson Memorial Hospital offers several types of opportunities for short-term observational learning experiences for high school, college and adult students pursuing healthcare careers. The process to apply for a shadow experience depends on the age of the applicant. Note: Robinson Memorial Hospital has a mandatory flu vaccination policy for all employees, volunteers, contracted workers and shadow students. Students wanting to shadow at Robinson Memorial must provide proof of receiving a flu vaccine during current flu season - either by a receipt showing the student's name and detail of service from the provider, an insurance benefits explanation or detailed note from a physician. Students can bring their vaccine documentation the morning of their assigned shadow exp...
http://robinsonmemorial.org/Main/Shadowing.aspx
*  [Haskell-cafe] System.Random.Shuffle fix
System.Random.Shuffle fix. System.Random.Shuffle fix friggin friggin frigginfriggins at gmail.com. Previous message: A small display problem using Helium Next message: System.Random.Shuffle fix Messages sorted by:. I was looking for a shuffling algorithm to shuffle mp3-playlists so was very happy to see System.Random.Shuffle: http://hackage.haskell.org/cgi-bin/hackage-scripts/package/random-shuffle-0.0.2 However I get errors,non-exhaustive patterns in function shufleTree or extractTree depending how I call it. Errors are at the bottom. I fixed it but I don't have the math skills to see if I perhaps broke it statistically ... Here is my fix, someone don't remember who, helped me a little : http://hpaste.org/fastcgi/hpaste.fcgi/view?id=2789#a2789 the shuffle at the end is with the fix. *Freet S.shuffle Loading package syb ... linking ... Loading package base-3.0.3.0 ... linking ... Loading package old-locale-1.0.0.1 ... linking ... Loading package old-time-1.0.0.1 ... linking ... Loading package random-1.0.0.1 ...
https://mail.haskell.org/pipermail/haskell-cafe/2009-March/058399.html
*  OriGene - LRRC2 (NM 024512) cDNA Clone
OriGene - LRRC2 NM 024512 cDNA Clone. cDNA Clones TrueORF cDNA Clones H/M/R Viral ORF Clones Destination Vector TrueClone Human TrueClone Mouse Organelle Marker Plasmids MicroRNA Tools Mutant and Variant Clones Plasmid Purification Kits Transfection Reagents Gene Synthesis Service. Home TrueClone LRRC2 Clone. LRRC2 NM 024512 Human cDNA Clone. SC310473 LRRC2 untagged -Human leucine rich repeat containing 2 LRRC2, NM 024512.2, 10ug $680 3 weeks. Reference Data RefSeq: NM 024512.2, NP 078788. Synonyms : leucine-rich repeat-containing 2; leucine rich repeat containing 2. More TrueClone Citations >>. LRRC2 Mouse Clones SKU Description Amount Price Shipping. MC211501 Lrrc2 untagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $380 3 weeks. MR216368 Lrrc2 Myc-DDK-tagged - Mouse leucine rich repeat containing 2 Lrrc2 10 ug $380 Next day. MG216368 Lrrc2 GFP-tagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $420 3 weeks. MR216368L1 Lenti ORF clone of Lrrc2 Myc-DDK-tagged - Mouse leucine ri...
http://origene.com/cdna/trueclone/accession/NM_024512/SC310473.aspx
*  JUST LIKE A WOMAN LYRICS - BOB DYLAN
just like a woman lyrics bob dylan sing lyrics bob dylan biography bob dylan albums bob dylan lyrics bob dylan just like a woman lyrics last updated pm sponsored links nobody feels any pain tonight as i stand inside the rain ev rybody knows that baby s got new clothes but lately i see her ribbons and her bows have fallen from her curls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl queen mary she s my friend yes i believe i ll go see her again nobody has to guess that baby can t be blessed till she finally sees that she s like all the rest with her fog her amphetamine and her pearls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl it s was raining from the first and i was dying there of thirst so i came in here and your long time curse hurts but what s worse is this pain in here i can t stay i...
http://sing365.com/music/Lyric.nsf/Just-Like-A-Woman-lyrics-Bob-Dylan/659E935998737E48482569690029ABC7
*  Gary Phillips eBooks | epub and pdf downloads | eBookMall
Gary Phillips eBooks. epub and pdf downloads. eBookMall. Search. Sponsored link: What is YOUR Secret Talent. - Take a Free Personality test. Gary Phillips eBooks Epub and PDF format. Gary Phillips eBooks. eBooks found: 15 Occupied Earth: Stories of Aliens, Resistance and Survival at all Costs. Richard Brewer. Gary Phillips Polis Books, October 2015. ISBN: 9781940610528 Format: ePub. List Price: $ 9.99 Our price: $ 7.99. Violent Spring. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087968 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Perdition, U.S.A. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087975 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Bad Night Is Falling. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087982 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Only the Wicked. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087951 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. The Extractors. Gary Phillips Stark Raving Group, M...
http://ebookmall.com/author/gary-phillips
*  "Beyond Stroke" Support Group | Events | Calendar of Events | Robinson Memorial Hospital
beyond stroke support group events calendar of events robinson memorial hospital about us foundation volunteer speaker s bureau press room contact us patients visitors medical services nursing find a doctor locations careers patient portal beyond stroke support group calendar view back to previous page support groups tuesday october pm pm description stroke survivors and caregivers will benefit from information presented by a variety of speakers as well as from sharing and support of their peers for information call jan bahle at location robinson medical arts building rmab n chestnut st ravenna ohio contact healtheducation rmh org phone visitors patient portal key phone numbers visiting hours wheelchair assistance wi fi at robinson memorial hospital locations robinson memorial hospital robinson medical arts building robinson professional center robinson health center at aurora robinson hea...
http://robinsonmemorial.org/Main/Calendar/659.aspx
*  Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine
... Home About Stories. Featured. BOR Featured. User Stories. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine 3. By Burnt Orange Report. on October 19, 2010. Bobby Phillips, Jr. Phillips states that he met Erwin Cain in October of 1977, when he was engaged to Erwin's sister, Gloria Cain. Attorney : Did you ever see Mr. Erwin Cain sell drugs. Phillips : Yeah, I've seen him deliver to his brother-in-law, to his brother, and to other people that came by the house when he was there. Attorney : Do you know where Thomas Cain, Jr. Attorney: which brother would that be. Phillips : Erwin. Attorney: Just to clarify, and let me repeat it's your statement that you have seen, in the past, a man you can identify as Erwin Cain use marijuana. Phillips : Yes. Attorney: Have you, and is it your statement also that you have seen him sell marijuana to someone. Phillips : Yeah. Phillips : It was usually just we used to go over to his h...
http://burntorangereport.com/diary/10880/deposition-of-bobby-phillips-states-that-erwin-cain-sold-cocaine
*  Anthony Robinson | dafont.com
Anthony Robinson. dafont.com. English. Fran ais. Espa ol. Deutsch. Italiano. Portugu s Login. Register. Themes New fonts Authors Top Forum FAQ Submit a font Tools. 4 matching requests on the forum Anthony Robinson 1. 2 3. Anthony Robinson. View profile Send a private message http://www.redbubble.com/people/anfa/portfolio 58 fonts - 4,331,510 downloads 2,129 yesterday. Preview. Fonts 10 20 50 100. Show variants. Size Tiny Small Medium Large Sort by Name Popularity Newer first. More options. Only as. Public domain / GPL / OFL. 100% Free. Free for personal use. Donationware. Shareware. Demo. Unknown Only fonts with. Accents. Euro. Newcastle by Anthony Robinson. in Fancy. Decorative 15,200 downloads 4 yesterday. 100% Free - 2 font files Download Donate to author. Static Buzz by Anthony Robinson. in Fancy. Distorted 104,264 downloads 4 yesterday. 100% Free Download Donate to author. Lasso Of Truth by Anthony Robinson. in Gothic. Modern 5,855 downloads 4 yesterday. 100% Free Download Donate to author. Punched by An...
http://dafont.com/anthony-robinson.d2796?page=3
*  Anthony Robinson | dafont.com
Anthony Robinson. dafont.com. English. Fran ais. Espa ol. Deutsch. Italiano. Portugu s Login. Register. Themes New fonts Authors Top Forum FAQ Submit a font Tools. 4 matching requests on the forum Anthony Robinson 1. 2 3. Anthony Robinson. View profile Send a private message http://www.redbubble.com/people/anfa/portfolio 58 fonts - 4,333,980 downloads 2,470 yesterday. Preview. Fonts 10 20 50 100. Show variants. Size Tiny Small Medium Large Sort by Name Popularity Newer first Reinitialize More options. Only as. Public domain / GPL / OFL. 100% Free. Free for personal use. Donationware. Shareware. Demo. Unknown Only fonts with. Accents. Euro. First by Anthony Robinson. in Techno. Sci-fi 12,112 downloads 3 yesterday. 100% Free Download Donate to author. Newcastle by Anthony Robinson. in Fancy. Decorative 15,203 downloads 3 yesterday. 100% Free - 2 font files Download Donate to author. New York Punk by Anthony Robinson. in Fancy. Destroy 20,765 downloads 3 yesterday. 100% Free Download Donate to author. Lasso Of T...
http://dafont.com/anthony-robinson.d2796?page=5&text=AUBURN
*  Phillip Phillips Ringtones Free Download - Ringtone Maker Audiko
... Sublime, Kendrick Lamar, DevilDriver, K. Michelle, Five Finger Death Punch. Mobile apps. Login. JavaScript must be enabled in order for you to use Audiko in standard view. A B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S. T. U. V. W. X. Y. Z. 0...9. Thousands of ringtones on Audiko.net. Here is a chance to download a free Phillip Phillips ringtones for your iPhone or mobile. Specify your favorite line from your favorite award-winning track - Beggin, Get Up Get Down, Unpack Your Heart, or any other track. You may download your ringtone both in the MP3 and m4a formats, supported by both mobile phones and iPhones. Just select a track, and choose free Phillip Phillips ringtones download option. Download Phillip Phillips Ringtones. Top free ringtones. Name. Gone, Gone, Gone. Home. Raging Fire. Alive Again. Face. Get Up Get Down. Searchlight. Volcano. U Got It Bad. We've Got Tonight. Albums. Behind the Light Deluxe Version. Phillip Phillips. Weve Got Tonight American Idol Performance Single. Phillip Phil...
http://audiko.net/ringtone/phillip-phillips
*  Coles Phillips
... 'Clarence Coles Phillips' October 1880June 13, 1927 was an American artist and illustrator who signed his early works 'C. Coles Phillips', but after 1911 worked under the abbreviated name, 'Coles Phillips'. He is known for his stylish images of women and a signature use of negative space in the paintings he created for advertisements and the covers of popular magazines. Early life Career Personal life Notes References External links. Early life. ' ' Michael Schau, Watson-Guptill, 1975, p. Image:Coles Phillips2 Life.jpg The work of Phillips quickly became popular with the 'Life' readers. Schau & Phillips 1975, p. Phillips' use of negative space allowed the viewer to "fill-in" the image; it also reduced printing costs for the magazine, as "the novelty of the technique and the striking design qualities masked the fact that 'Life' was getting by with single color or two-color covers in a day when full-color covers were de rigueur for the better magazines". Phillips worked in watercolor and always painted fro...
https://en.wikipedia.org/wiki/Coles_Phillips
*  Phillip Phillips News, Pictures, and Videos | TMZ.com
Idol Managers Claim John Mayer's ex-manager plotted to jack "American Idol" champ Phillip Phillips out from under his contract with the show -- according to a new lawsuit, but the bigger news is Phil is a READ MORE > - 9/21/2015 2:51 PM PDT. Phillip Phillips I Want to be Gone, Gone, Gone From My Idol Contract Phillip Phillips says "American Idol" producers are ruining his career ... American Idol Winner Phillip Phillips Mother Popped for DUI Phillip Phillips' mother Sheryl Phillips -- who appeared on several episodes of "American Idol" during Phillip's championship run last season -- was arrested for DUI earlier today, TMZ has READ MORE > - 963 days ago. READ MORE > - 1063 days ago. Phillip Phillips Family Idol Champ s Forcing Us To Sell Pawn Shop Phillip Phillips' family says the singer left them high and dry after winning "American Idol" -- refusing to help them out financially -- and now, they've been forced to sell their cherished READ MORE > - 1066 days ago. Phillip Phillips Crooks Smash In Pawn Shop, St...
http://tmz.com/person/phillip-phillips/?adid=cr_featured_saga_14
*  HERBICIDE-TOLERANT PLANTS - BASF AGROCHEMICAL PRODUCTS.B.V.
In addition, enzymes of this embodiment will also comprise one or more of a leucine, threonine, a valine, or alanine at position 1,781 Am, a glycine at position l,785 Am, a proline at position l,786 Am, an asparagine at position 1,811 Am, a proline at position l,824 Am, a cysteine or glycine at position l,999 Am, a cysteine or arginine at position 2,027 ' Am, a glycine at position 2,039 Am, an asparagine at position 2,041 Am, a phenylalanine, isoleucine or leucine at position 2,049 Am, a valine at position 2,059 Am, a leucine at position 2,074 Am, a leucine, isoleucine, methionine or additional valine at position 2,075 Am, a glycine or threonine at position 2,078 Am, a phenylalanine at position 2,079 Am, a glutamic acid at position 2,080 Am, a deletion at position 2,080 Am, a deletion at position 2,081 Am, an arginine, tryptophan, phenylalanine, glycine, histidine, lysine, leucine, serine, threonine, or valine at position 2,088 Am, a glutamic acid at position 2,095 Am, an alanine or serine at position 2,096 A...
http://sumobrain.com/patents/wipo/Herbicide-tolerant-plants/WO2011028836.html
*  Bimolecular fluorescence complementation
This was achieved by dissecting GFP into C- and N-terminal GFP fragments. In addition, this study was the first report of an ' in vivo ' technique, now known as the bimolecular fluorescence complementation BiFC assay, to provide insight into the structural basis of protein complex formation through detection of fluorescence caused by the assembly of fluorescent reporter protein fragments tethered to interacting proteins. Generally, YFP is recommended to serve as the reporter protein, cleaved at residue 155 N-terminal consisting of residues 1–154 and C-terminal consisting of residues 155–238 or residue 173 in particular, as these sets of fragments are highly efficient in their complementation when fused to many interacting proteins and they produce low levels fluorescence when fused to non-interacting proteins. It is suggested that each target protein is fused to both the N- and C-terminal fragments of the fluorescent reporter protein in turn, and that the fragments are fused at each of the N- and C-terminal e...
https://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation
*  Candice Olson Family Spaces ISBN 9781118276679 PDF epub | Candice Olson ebook | eBookMall
Candice Olson Family Spaces ISBN 9781118276679 PDF epub. Candice Olson ebook. eBookMall. Candice Olson Family Spaces. Candice Olson. Design : Interior Decorating. Digital Book format: ePub Adobe DRM. Buy ePub. List price: $ 19.99 Our price: $ 16.99. Popular TV star Candice Olson shows you how to create your dream family room Everybody wants their home's main living space to meet the needs of the whole family—and look great doing it. Chock full of tips and ideas, Candice Olson Family Spaces features hundreds of full-color, before-and-after photos of Candice's stunning family room makeovers. Explains how good design accommodates all members of the family and their daily activities Designs offer functionality, durability, and comfort without sacrificing style As a busy mother of two, Candice brings valuable insight to what a family really needs from the spaces they share Redecorating—or even just dreaming about decorating—can transform one's outlook on life, and Candice Olson Family Spaces is the catalyst to get...
http://ebookmall.com/ebook/candice-olson-family-spaces/candice-olson/9781118276679
*  Abner Phillips (1737-1813), p.2
Abner Phillips - Surry County, NC There were Phillips in Surry County, NC as early as 1771, according to tax lists. Lewis' Dist.: Abner PHILLIPS, 400 acres, 1 white poll. LEWIS to Abner PHILLIPS, 300 acres on north side of Yadkin River, east side Turkey Creek, for 75. LEWIS to Abner PHILLIPS, 100 acres on north side of Yadkin River at Turkey Creek, for 75. Phillips Dist.: Abner PHILLIPS, 400 acres, 2 white polls. 1790 Census, Surry Co., NC, p.186: ABNER PHILLIPS 3 white males under 16 2 white males over 16 3 white females no slaves Note: Abner's sons Cornelius PHILLIPS and Ephraim PHILLIPS are also listed in the 1790 Census of Surry Co., NC, p.183. 1800 Census, Surry Co., NC: ABNER PHILLIPS. 1810 Census, Surry Co., NC, p.193: ABNER PHILLIPS. In the name of God Amen this tenth day of November in year of our lord 1812 I ABNER PHILLIPS of Surry County State of North Carolina being sick and weak in Body but in perfect mind memory thanks be given to God For the Same Calling unto mind the mortality of my bo...
http://moonzstuff.com/phillips/abner1737_p2.html
*  "Dear Abby" Advice Columnist Pauline Phillips Dead at 94 - Us Weekly
... US Weekly. News. Photos. Style. Beauty. Moms. Body. Entertainment. Subscribe. Give a Gift. Us Weekly. Newsletter. Subscribe. ADVERTISEMENT. "Dear Abby" Advice Columnist Pauline Phillips Dead at 94. Celebrity News. Jan. Tweet. Columnist Abigail Van Buren attends Friends Of Mario Cuomo Fundraiser Dinner on November 30, 1989 at the Sheraton Center in New York City. Newspapers -- and readers -- the world over lost an icon Wednesday, Jan. 16, after "Dear Abby" advice columnist Pauline Phillips died in Minneapolis at the age of 94. Phillips had suffered from Alzheimer's disease for years, but was still dishing out advice to readers as recently as 2002, though her daughter Jeanne took on co-authoring duties in the mid-1990s. PHOTOS: Stars we lost in 2012. Phillips, who wrote under the pen name Abigail Van Buren, shared advice on everything from dating to snoring spouses for more than 40 years to a readership of more than 90 million newspaper readers. "They gave her a bunch of letters, thinking that they would n...
http://usmagazine.com/celebrity-news/news/dear-abby-advice-columnist-pauline-phillips-dead-at-94-2013171
*  Joanna Phillips | UCSF Medical Center
Joanna Phillips. UCSF Medical Center. About UCSF. Find a Doctor. Conditions Treatments. Conditions Treatments A-Z. Patient Education. Doctors. Find a Doctor. Getting to UCSF Medical Center. Careers at UCSF Medical Center. UCSF Medical Group. Home > Doctors > Joanna Phillips. Find a Doctor. Joanna Phillips, M.D. Joanna Phillips is a neuropathologist at UCSF Medical Center who helps diagnose neurologic disease through the examination of central nervous system tissue. As an academic neuropathologist, her goal is to improve the treatment of brain tumors through research and patient care. She completed a pathology residency at the University of Washington in Seattle, followed by a fellowship in neuropathology at UCSF. Phillips is a member of several professional organizations, including the American Society for Investigative Pathology, American Association of Neuropathologists, American Association for Cancer Research and Glycobiology Research and Training Program. Safaee M, Fakurnejad S, Bloch O, Clark AJ, Ivan M...
http://ucsfhealth.org/joanna.phillips
*  SMART: Secondary literature for GLUCA domain
smart secondary literature for gluca domain smart mode normal genomic schultz et al proc natl acad sci usa letunic et al nucleic acids res doi nar gku home setup faq about glossary what s new feedback secondary literature sources for gluca the following references were automatically generated lopez lc frazier ml su cj kumar a saunders gf mammalian pancreatic preproglucagon contains three glucagon related peptides proc natl acad sci u s a display abstract we have isolated cdna clones encoding bovine pancreatic preproglucagon twenty five putative preproglucagon clones were selected by screening clones of a fetal bovine pancreas cdna library with a synthetic oligodeoxynucleotide probe the probe was a mixture of synthetic base dna oligomers constructed to correspond to the six carboxyl terminal amino acids residues of mature glucagon restriction mapping of six of these clones suggested that they represented a single mrna species primary sequence analysis of one clone containing a base pair dna insert revealed tha...
http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=GLUCA
*  viruses share common: Topics by Science.gov
... Toggle navigation. Topics by Science.gov Home. Site Map. Index. Alerts. Help. Contact Us. About. Sample records for viruses share common. 1 2. 3. 4. 5. . . To gain insights into the role and specificity of hepaciviral nonstructural protein 2 NS2, which is required for HCV polyprotein processing and particle morphogenesis, we investigated whether NS2 structural and functional features are conserved between HCV and GBV-B. We found that GBV-B NS2, like HCV NS2, has cysteine protease activity responsible for cleavage at the NS2/NS3 junction, and we experimentally confirmed the location of this junction within the viral polyprotein. A model for GBV-B NS2 membrane topology was experimentally established by determining the membrane association properties of NS2 segments fused to green fluorescent protein GFP and their nuclear magnetic resonance structures using synthetic peptides as well as by applying an N-glycosylation scanning approach. Similar glycosylation studies confirmed the HCV NS2 organization. Togeth...
http://science.gov/topicpages/v/viruses share common.html
*  VPR: Andrea Phillips thanks President Obama, military for her husband's rescue
... .  Archive Home .  VPR.net Home . Andrea Phillips thanks President Obama, military for her husband's rescue. 04/13/09 5:42PM By Ross Sneyd.  MP3 .  Download MP3 . AP Photo/Toby Talbot. Andrea Phillips is flanked by her son, Daniel, and daughter, Mariah. Host A day after her husband was rescued from Somali pirates, Andrea Phillips said she wanted to thank friends, officials and especially President Obama for their support during the crisis. In a short news conference in South Burlington today, Phillips also said she had also been able to speak with her husband by telephone. VPR's Ross Sneyd has more. Sneyd Andrea Phillips has had the flu since a day before her husband's capture - and she's just now recovering. Since last week, when it became clear that Richard Phillips had given himself up to the pirates to protect the crew on his Merchant Marine ship, she's stayed out of the spotlight. She said her husband got a laugh out of her condition, knowing that she would be appeari...
http://vpr.net/news_detail/84680/andrea-phillips-thanks-president-obama-military-fo/
*  Protein superfamily
A 'protein superfamily' is the largest grouping clade of proteins for which common ancestry can be inferred see homology. Usually this common ancestry is based on structural alignment. and mechanistic similarity even though no sequence similarity is evident. 2 Superfamilies typically contain several protein families which show sequence similarity within the family. The term 'protein clan' is commonly used for protease superfamilies based on the MEROPS protease classification system. Identification Sequence homology. Structural homology. Mechanistic similarity. Evolutionary significance Examples Protein superfamily resources See also References. Sequence homology. sequence homology. Superfamily members typically show no detectable sequence homology. In the PA clan of protease s, for example, not a single residue is conserved through the superfamily, not even those in the catalytic triad. Conversely, the individual families that make up a superfamily are defined on the basis of their sequence alignment, for exa...
https://en.wikipedia.org/wiki/Protein_superfamily
*  Publications | NEB
FEBS Lett. Proc.Natl.Acad.Sci.USA J Parasitol. Proteins Mol.Cell.Biol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Protein Sci Proc. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J B...
https://neb.com/research/publications?page=3&start=30&num=10&sorting=Year&asc=False
*  Publications | NEB
Proc.Natl.Acad.Sci.USA Molecular Paradigms for Eradicating Helmenthic Parasites Exp.Parasitol. Appl.Environ.Microbiol., Trop.Med.Parasitol. Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Biochem J. NY Proc.Natl.Acad.Sci. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbio...
https://neb.com/research/publications?num=50&sorting=&asc=False
*  Publications | NEB
FEBS Lett. Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology B...
https://neb.com/sitecore/content/nebsg/home/research/publications?Page=2&sorting=Year
*  Par stability determinant
... notoc the par stability determinant is a bp locus of the pad plasmid which encodes a type i toxin antitoxin system in enterococcus faecalis it was the first such plasmid addiction module to be found in gram positive bacteria the par locus contains two gene s fst which encodes a amino acid toxic protein and a gene for rnaii the small rna anti toxin which inhibits fst translation the two genes are found on opposite dna strands and share a region which is where they are thought to have an antisense interaction their rna secondary structure s have been predicted computationally the complementary regions appear to be presented on exposed loops for interaction par maintains pad by means of post segregational killing psk if a daughter cell does not inherit the par locus the unstable rnaii will quickly degrade leaving the long lived fst toxin to damage or kill the daughter cell see also references further reading external links see also hok sok system references further reading external links category antisense ...
https://en.wikipedia.org/wiki/Par_stability_determinant
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?Page=29&asc=False
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?Page=55
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?page=50&start=500&num=10
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/sitecore/content/nebsg/home/research/publications?Page=47
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/sitecore/content/nebsg/home/research/publications?page=52&start=520&num=10&asc=False
*  .. Post navigation .. Happy Birthday Duncan Phillips .. Post navigation
post navigation square dancing last chance o is for origin story happy birthday duncan phillips posted on june by sarah osborne bender clockwise from top left milton avery black sea oil on canvas x in the phillips collection washington d c acquired robert motherwell in white and yellow ochre oil charcoal ink tempera and paper collage on paper x in the phillips collection washington d c acquired alexander calder only only bird tin cans and wire x x in the phillips collection washington d c acquired karl knaths the blue heron at the tide wash oil on canvas x in the phillips collection washington d c acquired i had the pleasure of speaking last night to alice phillips swistel great niece of duncan phillips who was born today in it s not surprising that the conversation came around to her memories of him though i ve worked here for over five years and handled many of phillips s belongings his j...
http://blog.phillipscollection.org/2012/06/26/happy-birthday-duncan-phillips/
*  Consensus sequence
In molecular biology and bioinformatics, the 'consensus sequence' is the calculated order of most frequent residues, either nucleotide or amino acid, found at each position in a sequence alignment. It represents the results of multiple sequence alignment s in which related sequences are compared to each other and similar sequence motifs are calculated. Biological significance Sequence analysis Notation. Thus a consensus sequence is a model for a putative DNA binding site : it is obtained by aligning all known examples of a certain recognition site and defined as the idealized sequence that represents the predominant base at each position. Any mutation allowing a mutated nucleotide in the core promoter sequence to look more like the consensus sequence is known as an 'up mutation'. On the contrary, mutations that destroy conserved nucleotides in the consensus sequence are known as 'down mutations'. These types of mutations down-regulate transcription since RNA polymerase can no longer bind as tightly to the cor...
https://en.wikipedia.org/wiki/Consensus_sequence
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. Nature Structure Methods Mol.Biol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front Genet Curr Protoc Mol Biol Methods Mol Biol ...
https://neb.com/research/publications?num=100&sorting=Journal&asc=False
*  Bill Robinson to sit out 27 months - Harness Racing Newsroom - USTA - USTROTTING
... WHAT IS THE USTA. USTA. USTA Home. Rule Book. Rule Book PDF. Rule Change Proposals. Rule Change Proposals. Ask the USTA. This Is Harness Racing. A Winning Combination. Rule Book. Rule Book PDF. Rule Change Proposals. Stakes Guide. Stakes Event Guide. USTA Youth. HORSE SERVICES. Racing Entries/Results. Racing Home. TrackMaster Race Programs. Bill Robinson to sit out 27 months Tuesday, April 20, 2004 - by Harness Racing Communications, a division of the USTA Tweet. Trainer Bill Robinson will serve suspensions totaling 27 months and pay fines totaling C$125,000 under an agreement with the Ontario Racing Commission announced Tuesday by the ORC. On April 16, the Ontario Superior Court of Justice Divisional Court dismissed Robinson's application for judicial review regarding a C$50,000 fine and 10-month suspension because of a positive Class III drug test on the horse Artistic. Robinson served approximately four months of the suspension for the Artistic ruling, and will serve another six months and one week un...
http://xwebapp.ustrotting.com/absolutenm/templates/article.aspx?articleid=2247&zoneid=1
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. Proteins Mol.Cell.Biol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front Genet Curr Protoc Mol Biol Methods Mol Biol Eur J...
https://neb.com/research/publications?Page=32&sorting=Journal&asc=False
*  SMART: CDC37 C domain annotation
smart cdc c domain annotation smart mode normal genomic schultz et al proc natl acad sci usa letunic et al nucleic acids res doi nar gku home setup faq about glossary what s new feedback cdc c cdc c terminal domain smart accession number sm description cdc is a protein required for the activity of numerous eukaryotic protein kinases this domains corresponds to the c terminal domain whose function is unclear it is found c terminal to the hsp chaperone heat shocked protein binding domain pf and the n terminal kinase binding domain of cdc pubmed interpro abstract ipr cdc is a protein required for the activity of numerous eukaryotic protein kinases this entry corresponds to the c terminal domain whose function is unclear it is found c terminal to the hsp chaperone heat shock protein binding domain ipr and the n terminal kinase binding domain of cdc ipr family alignment view or chroma format clustalw format msf format fasta format pir format there are cdc c domains in proteins in smart s nrdb database click on the...
http://smart.embl-heidelberg.de/smart/do_annotation.pl?ACC=SM01069&BLAST=DUMMY
*  Nucleotide (Weighted) Links for PubMed (Select 17242370) - Nucleotide - NCBI
Nucleotide Weighted Links for PubMed Select 17242370 - Nucleotide - NCBI. NCBI. BLAST Basic Local Alignment Search Tool. GenBank. BLAST Basic Local Alignment Search Tool. Search database All Databases Assembly BioProject BioSample BioSystems Books ClinVar Clone Conserved Domains dbGaP dbVar Epigenomics EST Gene Genome GEO DataSets GEO Profiles GSS GTR HomoloGene MedGen MeSH NCBI Web Site NLM Catalog Nucleotide OMIM PMC PopSet Probe Protein Protein Clusters PubChem BioAssay PubChem Compound PubChem Substance PubMed PubMed Health SNP SRA Structure Taxonomy ToolKit ToolKitAll ToolKitBook UniGene Search term. Format Summary GenBank GenBank full FASTA ASN.1 XML INSDSeq XML TinySeq XML Feature Table Accession List GI List. Sort by Default order Accession Date Modified Date Released Organism Name Taxonomy ID. Mus musculus guanine nucleotide binding protein G protein, alpha inhibiting 2 Gnai2, mRNA. Mus musculus adult inner ear cDNA, RIKEN full-length enriched library, clone:F930015A20 product:guanine nucleotide bind...
http://ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore_weighted&from_uid=17242370
*  LARGE POOL SIZE
... Oracle Forums. Oracle Database Administration. LARGE POOL SIZE. Thread: LARGE POOL SIZE. View Profile. View Forum Posts. i want to know how these large pool will use in ORACLE. Reply With Quote. View Profile. View Forum Posts. Senior Advisor. You can size the large pool with LARGE POOL SIZE LARGE POOL MIN ALLOC parameters in init.ora If the size is 0, the large pool is not set. Reply With Quote. View Profile. View Forum Posts. Reply With Quote. View Profile. View Forum Posts. Join Date May 2000 Location ATLANTA, GA, USA Posts 3,135. LARGE POOL SIZE does not improve I/O slave processes. If this area is set, then it will be used for PL/SQL procedures. In general, 10 % of SHARED POOL SIZE is set for this parameter. Reply With Quote. View Profile. View Forum Posts. Originally posted by tamilselvan LARGE POOL SIZE does not improve I/O slace processes. If this area is set, then it will be used for PL/SQL procedures. In general, 10 % of SHARED POOL SIZE is set for this parameter. Large pool is not a part of SHA...
http://dbasupport.com/forums/showthread.php?16303-LARGE_POOL_SIZE&p=65753
*  Olson family statement, 1975
... The Frank Olson. Legacy Project. FAMILY STATEMENT, 1975. FAMILY STATEMENT BY THE WIFE AND CHILDREN OF FRANK R. OLSON Alice Wicks Olson Eric Wicks Olson Lisa Wicks Olson Hayward Nils Wicks Olson Released in a press conference July 10, 1975. We are the family of Frank R. Olson. Frank Olson a civilian biochemist working for the United States Army died shortly after midnight on November 28, 1953, when he plunged to his death from a window on the 10th floor of the Hotel Statler in New York. The death certificate states that Frank Olson jumped or fell and cites multiple fractures, shock and hemorrhage as the causes of death. An employee of the Central Intelligence Agency had been with Frank Olson in his hotel room the night he died. This man was accompanying Frank Olson in his hotel room the night he died. This man was accompanying Frank Olson when Olson was taken to New York to consult a psychiatrist. Olson s widow was later told that her husband s escort had awakened about 1:30 A.M. to see Olson going at a f...
http://frankolsonproject.org/Statements/FamilyStatement1975.html
*  Search results for query PF00558 - Protein Data Bank Japan
PDB: 9 results Info pages: 0 results Status search: 0 results Chemie search: 0 results ✖. STRUCTURE OF THE CHANNEL-FORMING TRANS-MEMBRANE DOMAIN OF VIRUS PROTEIN "U" VPU FROM HIV-1. Authors: Park, S.H., Mrse, A.A., Nevzorov, A.A., Mesleh, M.F., Oblatt-Montal, M., Montal, M., Opella, S.J. Cite: Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" Vpu from HIV-1 J.Mol.Biol., 333, 2003. STRUCTURE OF THE CHANNEL-FORMING TRANS-MEMBRANE DOMAIN OF VIRUS PROTEIN "U" VPU FROM HIV-1. Authors: Park, S.H., Mrse, A.A., Nevzorov, A.A., Mesleh, M.F., Oblatt-Montal, M., Montal, M., Opella, S.J. Cite: Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" Vpu from HIV-1 J.Mol.Biol., 333, 2003. STRUCTURE OF THE CHANNEL-FORMING TRANS-MEMBRANE DOMAIN OF VIRUS PROTEIN "U" VPU FROM HIV-1. Authors: Park, S.H., Mrse, A.A., Nevzorov, A.A., Mesleh, M.F., Oblatt-Montal, M., Montal, M., Opella, S.J. Cite: Three-dimensional structure of the channel-forming tr...
http://pdbj.org/mine/search?query=PF00558
*  SCOP: Protein: Class II MHC beta chain, C-terminal domain from Human (Homo sapiens), HLA-DR group [T
SCOP: Protein: Class II MHC beta chain, C-terminal domain from Human Homo sapiens , HLA-DR group. Structural Classification of Proteins Protein: Class II MHC beta chain, C-terminal domain from Human Homo sapiens , HLA-DR group. Lineage: Root: scop Class: All beta proteins Fold: Immunoglobulin-like beta-sandwich sandwich; 7 strands in 2 sheets; greek-key some members of the fold have additional strands Superfamily: Immunoglobulin uperfamily Family: C1 set domains antibody constant domain-like Protein: Class II MHC beta chain, C-terminal domain Species: Human Homo sapiens , HLA-DR group. SQ P04229 30-219 probably orthologous to the mouse I-E group PDB Entry Domains:. 1fv1. complexed with ace, nag region b:93-190. complexed with ace; mutant region b:93-190. automatically matched to d1bx2b1 region b:93-190. automatically matched to d1d5xb1 complexed with dio, epe, so4, zn region b:93-190. automatically matched to d1d5xb1 complexed with 4dp, ace; mutant region b:93-190. mutant region b:93-190. mutant region b:93-1...
http://scop.mrc-lmb.cam.ac.uk/scop/data/scop.b.c.b.b.c.gg.html
*  Genetic regulatory mechanisms and carcinogenesis.
... Enter Your Search: Search tips Search criteria Articles Journal titles. Public Health Rep. Page Browse. Bates, R. Public Health Rep. PMCID: PMC1919899. Genetic regulatory mechanisms and carcinogenesis. H V Gelboin and R. Bates. Full text Full text is available as a scanned copy of the original print version. Links to PubMed are also available for Selected References. Selected References. These references are in PubMed. Mammary cancer induced by a single feeding of polymucular hydrocarbons, and its suppression. Cancer Res. 1958 Jul; 18 6 :725–729. Cancer Res. 1962 Jun; 22 :525–548. J Natl Cancer Inst. 1957 Jun; 18 6 :769–778. Cancer Res. IDENTITY OF DNA AND DIVERSITY OF MESSENGER RNA MOLECULES IN NORMAL MOUSE TISSUES. Proc Natl Acad Sci U S A. 1964 Oct; 52 :915–922. THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA. Proc Natl Acad Sci U S A. 1963 Oct; 50 :730–738. Proc Natl Acad Sci U S A. Proc Natl Acad Sci U S A. V Evidence for induction of enzyme synthesis in the rat by 3-methylcholanthrene. ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC1919899/
*  Maps: pUC18, pUC19 DNA
Home Contacts Order Catalog Support. pUC18, pUC19 pUC18. GenBank/EMBL sequence accession number L09136. pUC19. GenBank/EMBL sequence accession number L09137. Restriction sites of the pUC18 DNA Please note that the pUC19 nt sequence deposited under EMBL accession number X02514 is exactly the same as L09137 GenBank/EMBL, while X02514 GenBank has two errors. CAP protein binding site 591-554 compl. lac repressor binding site 507-487 compl. pUC18 and pUC19 vectors are small, high copy number, E.coli plasmids, 2686 bp in length. pUC18/19 plasmids contain: 1 the pMB1 replicon rep responsible for the replication of plasmid source plasmid pBR322. The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; 2 bla gene, coding for beta-lactamase that confers resistance to ampicillin source plasmid pBR322. It differs from that of pBR322 by two point mutations; 3 region of E.coli operon lac containing CAP protein binding site, promoter Plac, lac repressor binding...
http://dwb4.unl.edu/Chem/CHEM869N/CHEM869NLinks/fermentas.com/TechInfo/NucleicAcids/MappUC1819.htm
*  Tetracyclin repressor-like, C-terminal domain superfamily
Biological Process BP regulation of RNA metabolic process 0.0008117 1.109 -- DIRECT. Biological Process BP regulation of RNA biosynthetic process 0.00000000001558 1.154 -- DIRECT. Biological Process BP regulation of macromolecule metabolic process 0.1063 0.7873 -- INHERITED FROM : regulation of RNA metabolic process |. Biological Process BP regulation of metabolic process 0.00002713 0.6426 Least Informative DIRECT. Biological Process BP regulation of macromolecule biosynthetic process 0.0007777 1.059 Moderately Informative DIRECT. Biological Process BP regulation of cellular biosynthetic process 0.0335 0.9894 Moderately Informative INHERITED FROM : regulation of cellular macromolecule biosynthetic process |. Biological Process BP regulation of nucleobase-containing compound metabolic process 0.01401 0.9736 Moderately Informative INHERITED FROM : regulation of RNA metabolic process |. Molecular Function MF molecular_function 1 0 -- INHERITED FROM : nucleic acid binding |. Molecular Function MF binding 0.4364 0...
http://supfam.cs.bris.ac.uk/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF48498
*  PlantTFDB - Plant Transcription Factor Databases @ CBI, PKU
bHLH Family Introduction The basic/helix-loop-helix bHLH proteins are a superfamily of transcription factors that have been well characterized in nonplant eukaryotes, especially in mammalian systems, in which considerable structural, functional, and phylogenetic analyses have been performed. The data indicate that bHLH proteins are important regulatory components in transcriptional networks in these systems, controlling a diversity of processes from cell proliferation to cell lineage establishment. The basic region, located at the N-terminal end of the domain, is involved in DNA binding and consists of 15 amino acids with a high number of basic residues. The HLH region, at the C-terminal end, functions as a dimerization domain and is constituted mainly of hydrophobic residues that form two amphipathic -helices separated by a loop region of variable sequence and length. Outside of the conserved bHLH domain, these proteins exhibit considerable sequence divergence. Cocrystal structural analysis has shown that th...
http://planttfdb.cbi.pku.edu.cn/family.php?fam=bHLH
*  .. [UPDATE] R. Kelly Hospitalized Due to ‘Strange Illness’ .. ************************** .. Oh
R. Kelly Hospitalized Due to ‘Strange Illness’. June 27th, 2012, lilD. . UPDATE. R Kelly’s people have said, “Initial indications are that he is suffering from complications from surgery he had last year to treat an abscess on his vocal cords.”. Because of said illness, Kells has had to cancel appearances on both the “Jimmy Fallon Show” and NBC’s “Today Show.”. His reps added: “It is unclear how long Kelly may be sidelined.”. The Wheard: TMZ. **************************. Aww man…and just when he was getting ready to launch his book tour. Did I so sund like I care…. Anywho, R. Kelly has been hospitalized due to some “strange illness,” so he’s had to postpone his album launching party in New York. One of R. Kellly’s reps said the date was postponed  “due to a health emergency that has sidelined Mr. Kelly in Chicago.”  No other information at this time. Wonder if it’s his voice. Remember when had that surgery when he lost his voice. Hmm…. The Wheard: Complex. I’m lilD, and that’s the Word Eye Heard. So many ...
http://thewordeyeheard.com/2012/06/27/r-kelly-hospitalized-due-to-strange-illness/
*  eIF-2-alpha, C-terminal domain superfamily
... Home > SCOP hierarchy > eIF-2-alpha, C-terminal domain superfamily. SEARCH Keyword search. Sequence search. BROWSE Organisms. Taxonomy. Statistics. SCOP. Hierarchy. Ontologies. GO. EC. Phenotype TOOLS Compare genomes. Phylogenetic trees. Web services. Downloads. ABOUT Description. Publications. Documentation HELP User support. Contact us. Email list. Sitemap. Structural Classification. Genome Assignments. Sequence Alignments. Domain Combinations. Taxonomic Distribution. eIF-2-alpha, C-terminal domain superfamily. SCOP classification Root: SCOP hierarchy in SUPERFAMILY 11. Class: Alpha and beta proteins a+b 376. Fold: Ferredoxin-like 59. Superfamily : eIF-2-alpha, C-terminal domain. Families: eIF-2-alpha, C-terminal domain. Superfamily statistics. Genomes 646 Uniprot 2014 06 PDB chains SCOP 1.75. Domains 757 1,028 3. Proteins 755 1,025 3. Functional annotation General category Information. Detailed category Translation. Document:. Function annotation of SCOP domain superfamilies. Gene Ontology high-covera...
http://supfam.cs.bris.ac.uk/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF110993
*  Dylan Bob Tabs - Just Like A Women Tabs (ver. 1)
Dylan Bob Tabs - Just Like A Women Tabs ver. 1 Home. D Dylan Bob. Dylan Bob, Just Like A Women Tabs ver.1. Artists: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #. Best songs All Of Me John Legend Love Me Like You Do Ellie Goulding Take Me To Church Hozier See You Again Wiz Khalifa Let Her Go Passenger Riptide Vance Joy Lay Me Down Sam Smith Stay With Me Sam Smith Four Five Seconds Rihanna Hallelujah Jeff Buckley. Follow us on. Just Like A Women Tabs ver.1. Dylan Bob Tabs. Rating: 4/ 5 4 votes. Versions:. Tabs. Chords. Version 1 4. ×. Print Tabs too difficult. Try these video lessons and learn fast. JUST LIKE A WOMAN by Bob Dylan C F G C Nobody feels any pain C F G C Tonight as I stand inside the rain F G F G Everybody knows that baby's got new clothes F Em Dm C F G But lately I see her ribbons and her bows Am C G Have fallen from her curls C Em F She takes just like a woman, yes she does C Em F She makes love just like a woman, yes she does C Em F And she aches just like a woman G C But she breaks jus...
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*  James - ( Just Like Fred Astaire tab )
James - Just Like Fred Astaire tab. Just Like Fred Astaire tab. James tabs. Song name Band name Song name. # A B C D E F G H I J K L M N O P Q R S T U V W X Y Z. Home / J / James / Just Like Fred Astaire tab. Print this tab. Listen to Just Like Fred Astaire. James - Just Like Fred Astaire tab. Highlighted Show chord diagrams. Doctor, What is happening to me. Palpitations My mind's diseased Even my v ision is impaired I'm losing my hair Cos when I hold her in my arms I feel like Fred As taire Lovesick My temperature's high Just met a girl Who believes we can fly[ I'm A millionaire .. And when I hold her in my arms I feel like Fred As taire. I believe in happiness I believe in Love I believe she fell to earth from somewhere high above I believe in Hollywood Don't believe that Love must bring despa ir Cos when I hold her in my arms I'm just like Fred Astaire He said love is just a disease A plague for the na ive These days no one be lieves Meteors may strike the earth Nations live and die I'm the boy who got the...
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*  [Bioperl-l] Degenerate primer calculation
Degenerate primer calculation. Degenerate primer calculation Samantha Thompson Samantha.Thompson at greenbiologics.com. Previous message: Degenerate primer calculation Next message: About to tag the last RC... Messages sorted by:. -----Original Message----- From: Sara Kalla Sent: 12 January 2009 20:10 To: Samantha Thompson Cc: bioperl-l List Subject: Re: Degenerate primer calculation Samantha Thompson wrote: -----Original Message----- From: Chris Fields. Sent: 08 December 2008 16:41 To: Samantha Thompson Cc: bioperl-l List Subject: Re: Degenerate primer calculation. On Dec 8, 2008, at 9:59 AM, Samantha Thompson wrote: Hi, I also have another similar sequence analysis/primer problem. What I'd like to do is produce degenerate primers from amino acid sequences. amino acid sequence derived as a consensus from multiple the alignment of homologous proteins and converts them into degenerate codons and then that degenerate primer actually several primers synthesised with different bases pooled together, in order to s...
http://bioperl.org/pipermail/bioperl-l/2009-January/028912.html
*  .. SmokeyRobinson .. Smokey Robinson & The Miracles Co-Founder Bobby Rogers Dead at Age 73 .. Presi
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http://theboombox.com/tags/SmokeyRobinson/
*  Products Archives - Phillips Pet Food & Supplies - Phillips Pet Food & Supplies
Products Archives - Phillips Pet Food Supplies - Phillips Pet Food Supplies. Products. Vendors. Events. Phillips News. Industry News. Vendors Customers Online Store. Products. Products. Eshopps 3rd Generation Sumps and Refugiums Posted on 8/5/15 by Emma Godfrey Newly designed for your tank come the Ehsopps 3rd Generation sumps and refugiums. The Eshopps 3rd Generation Reef Sump is extremely quiet, even virtually silent during operation due to the spill-over design in the micron bag compartment. It is designed for reef tanks, and its ease of installation makes it perfect for both beginner and Continue Reading. Products. The RAWZ Difference Posted on 7/31/15 by Emma Godfrey If you’re seeking a dog or cat food that does things differently, consider the new RAWZ kibble. RAWZ cat and dog food contains no rendered meals or processed ingredients, and its only protein source is dehydrated chicken. RAWZ chicken is steamed at low Continue Reading. Products. Walk In Comfort with Wigzi Gel Leashes Posted on 7/17/15 by Em...
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*  Patent US5158935 - Human epidermal growth factor having substitution at position 11 - Google Patents
We have now found that one may improve the storage life of EGF without diminishing its biological activity by replacing the Asp at position 11 with a different amino acid, preferably Glu, Asn, Gln, Ala, or Lys, more preferably Glu, or Asn, and most preferably Glu. Human epidermal growth factor having substitution at position 11 US 5158935 A Abstract We have now found that one may improve the storage life of EGF without diminishing its biological activity by replacing the Asp at position 11 with a different amino acid, preferably Glu, Asn, Gln, Ala, or Lys, more preferably Glu, or Asn, and most preferably Glu. The amino acid sequence of human EGF is: Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys Trp Trp Glu Leu Arg This sequence was published by H. Murine EGF has the sequence differences from hEGF underlined : Asn Ser Tyr Pro Gly Cys Pro Ser Ser Tyr Asp Gly Tyr Cys L...
http://google.com/patents/US5158935?dq=7,446,777
*  Protein–DNA interaction site predictor
... Characteristics of such binding sites may be used for predicting DNA-binding sites from the structural and even sequence properties of unbound proteins. First attempt to use sequence and evolutionary features to predict DNA-binding sites in proteins was made by Ahmad et al. It is based on the chemical-physical properties of the residue and its environment, predicted structural features and evolutionary data. Prediction of DNA-binding residues from sequence Bioinformatics 23 13 :i347-53 2007. 3 'DNABindR' predicts DNA binding sites from amino acid sequences using machine learning algorithms. Predicting DNA-binding sites of proteins from amino acid sequence. "BindN: a web-based tool for efficient prediction of DNA and RNA binding sites in amino acid sequences." Nucleic Acids Research. 6 'BindN+', an upgraded version of BindN, applies support vector machine s SVMs to sequence-based prediction of DNA or RNA-binding residues from biochemical features and evolutionary information. "DP-Bind: a web server for se...
https://en.wikipedia.org/wiki/Protein–DNA_interaction_site_predictor
*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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*  Rocky Patel Cigars | Gotham Cigars
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DNA binding site: DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.Coles PhillipsProximity ligation assay: Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions and modifications with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution and objectively quantified in unmodified cells and tissues.Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.GC box: In molecular biology, a GC box is a distinct pattern of nucleotides found in the promoter region of some eukaryotic genes upstream of the TATA box and approximately 110 bases upstream from the transcription initiation site. It has a consensus sequence GGGCGG which is position dependent and orientation independent.WIN 56,098: WIN 56,098 is a chemical that is considered to be an aminoalkylindole derivative. It is a tricyclic aryl derivative that acts as a competitive antagonist at the CB2 cannabinoid receptor.Reaction coordinatePituitary-specific positive transcription factor 1: POU domain, class 1, transcription factor 1 (Pit1, growth hormone factor 1), also known as POU1F1, is a transcription factor for growth hormone.Burst kinetics: Burst kinetics is a form of enzyme kinetics that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase".DNA-binding proteinDatabase of protein conformational diversity: The Database of protein conformational diversity (PCDB) is a database of diversity of protein tertiary structures within protein domains as determined by X-ray crystallography. Proteins are inherently flexible and this database collects information on this subject for use in molecular research.FERM domain: In molecular biology, the FERM domain (F for 4.1 protein, E for ezrin, R for radixin and M for moesin) is a widespread protein module involved in localising proteins to the plasma membrane.Eukaryotic transcription: Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of RNA replica. Gene transcription occurs in both eukaryotic and prokaryotic cells.Ligand (biochemistry): In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose. In protein-ligand binding, the ligand is usually a signal-triggering molecule binding to a site on a target protein.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.Ethyl groupList of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.Phase problem: In physics the phase problem is the name given to the problem of loss of information concerning the phase that can occur when making a physical measurement. The name itself comes from the field of x-ray crystallography, where the phase problem has to be solved for the determination of a structure from diffraction data.Enhancer (genetics)CS-BLASTRNA transfection: RNA transfection is the process of deliberately introducing RNA into a living cell. RNA can be purified from cells after lysis or synthesized from free nucleotides either chemically, or enzymatically using an RNA polymerase to transcribe a DNA template.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Repressor: In molecular genetics, a repressor is a DNA- or RNA-binding protein that inhibits the expression of one or more genes by binding to the operator or associated silencers. A DNA-binding repressor blocks the attachment of RNA polymerase to the promoter, thus preventing transcription of the genes into messenger RNA.Transmembrane domain: Transmembrane segment usually denotes a single transmembrane alpha helix of a transmembrane protein, also known as an integral protein.http://www.Triparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.Nucleic acid structure: Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar.Beef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.Affinity label: Affinity labels are molecules similar in structure to a particular substrate for a specific enzyme and are considered to be a class of enzyme inhibitors. These molecules covalently modify active site residues in order to reveal the active site and thus elucidate its structure.Cell membraneChIP-exo: ChIP-exo is a chromatin immunoprecipitation based method for mapping the locations at which a protein of interest (transcription factor) binds to the genome. It is a modification of the ChIP-seq protocol, improving the resolution of binding sites from hundreds of base pairs to almost one base pair.Hypersensitive site: In genetics a hypersensitive site is a short region of chromatin and is detected by its super sensitivity to cleavage by DNase I and other various nucleases (DNase II and micrococcal nucleases). In a hypersensitive site, the nucleosomal structure is less compacted, increasing the availability of the DNA to binding by proteins, such as transcription factors and DNase I.Mature messenger RNA: Mature messenger RNA, often abbreviated as mature mRNA is a eukaryotic RNA transcript that has been spliced and processed and is ready for translation in the course of protein synthesis. Unlike the eukaryotic RNA immediately after transcription known as precursor messenger RNA, it consists exclusively of exons, with all introns removed.Short linear motifSpecificity constant: In the field of biochemistry, the specificity constant (also called kinetic efficiency or k_{cat}/K_{M}), is a measure of how efficiently an enzyme converts substrates into products. A comparison of specificity constants can also be used as a measure of the preference of an enzyme for different substrates (i.Calcium signaling: Calcium ions are important for cellular signalling, as once they enter the cytosol of the cytoplasm they exert allosteric regulatory effects on many enzymes and proteins. Calcium can act in signal transduction resulting from activation of ion channels or as a second messenger caused by indirect signal transduction pathways such as G protein-coupled receptors.Spatiotemporal gene expressionAutoradiograph: An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g.CpG OligodeoxynucleotideLattice protein: Lattice proteins are highly simplified computer models of proteins which are used to investigate protein folding.Spectrofluorometer: A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength, or a scan is performed to record the intensity versus wavelength, also called an emission spectra.Chemically induced dimerization: Chemically Induced Dimerization (CID) is a biological mechanism in which two proteins bind only in the presence of a certain small molecule, enzyme or other dimerizing agent. Genetically engineered CID systems are used in biological research to control protein localization, to manipulate signalling pathways and to induce protein activation.Allosteric regulationMagnesium acetateSEA Native Peptide LigationNew Zealand rabbitMargaret Jope: Margaret Jope (1913–2004) was a Scottish biochemist, born as Henrietta Margaret Halliday in Peterhead, Scotland.Inhibitor protein: The inhibitor protein (IP) is situated in the mitochondrial matrix and protects the cell against rapid ATP hydrolysis during momentary ischaemia. In oxygen absence, the pH of the matrix drops.Alkaliphile: Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline (pH roughly 8.5-11) environments, growing optimally around a pH of 10.Matrix model: == Mathematics and physics ==DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Standard enthalpy of formation: The standard enthalpy of formation or standard heat of formation of a compound is the change of enthalpy during the formation of 1 mole of the compound from its constituent elements, with all substances in their standard states at 1 atmosphere (1 atm or 101.3 kPa).Zuotin: Z-DNA binding protein 1, also known as Zuotin, is a Saccharomyces cerevisiae yeast gene.Point mutationBaby hamster kidney cell: Baby Hamster Kidney fibroblasts (aka BHK cells) are an adherent cell line used in molecular biology.Escherichia coli (molecular biology): Escherichia coli (; commonly abbreviated E. coli) is a gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms).Spin–lattice relaxation in the rotating frame: Spin–lattice relaxation in the rotating frame is the mechanism by which Mxy, the transverse component of the magnetization vector, exponentially decays towards its equilibrium value of zero, under the influence of a radio frequency (RF) field in nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI). It is characterized by the spin–lattice relaxation time constant in the rotating frame, T1ρ.PSI Protein Classifier: PSI Protein Classifier is a program generalizing the results of both successive and independent iterations of the PSI-BLAST program. PSI Protein Classifier determines belonging of the found by PSI-BLAST proteins to the known families.Molar mass distribution: In linear polymers the individual polymer chains rarely have exactly the same degree of polymerization and molar mass, and there is always a distribution around an average value. The molar mass distribution (or molecular weight distribution) in a polymer describes the relationship between the number of moles of each polymer species (Ni) and the molar mass (Mi) of that species.

(1/81452) Extra-vesicular binding of noradrenaline and guanethidine in the adrenergic neurones of the rat heart: a proposed site of action of adrenergic neurone blocking agents.

1 The binding and efflux characteristics of [14C]-guanethidine and [3H]-noradrenaline were studied in heart slices from rats which were pretreated with reserpine and nialamide. 2 Binding of both compounds occurred at extra-vesicular sites within the adrenergic neurone. After a brief period of rapid washout, the efflux of [14C]-guanethidine and [3H]-noradrenaline proceeded at a steady rate. The efflux of both compounds appeared to occur from a single intraneuronal compartment. 3 (+)-Amphetamine accelerated the efflux of [14C]-noradrenaline; this effect was inhibited by desipramine. 4 Unlabelled guanethidine and amantadine also increased the efflux of labelled compounds. Cocaine in high concentrations increased slightly the efflux of [14C]-guanethidine but not that of [3H]-noradrenaline. 5 Heart slices labelled with [3H]-noradrenaline became refractory to successive exposures to releasing agents although an appreciable amount of labelled compound was still present in in these slices. 6 It is suggested that [14C]-guanethidine and [3H]-noradrenaline are bound at a common extravesicular site within the adrenergic neurone. Binding of guanethidine to the extra-vesicular site may be relevant to its pharmacological action, i.e., the blockade of adrenergic transmission.  (+info)

(2/81452) The bioavailability, dispostion kinetics and dosage of sulphadimethoxine in dogs.

The disposition kinetics of sulphadimethoxine were studied in six normal beagle dogs after intravenous injection of a single dose (55 mg/kg). The median (range) distribution and elimination half times of the drug were 2.36 (2.06-3.35) hours and 13.10 (9.71-16.50) hours, respectively. Total body clearance of the drug had a median value of 21.7 ml/kg/h and a mean value of 21.4 ml/kg/h. While the overall tissue to plasma level ratio (k12/k21) of the drug was 0.55 after distribution equilibrium had been attained, analogue computer simulated curves showed that at 24 hours the fractions (percentage) of the dose in the central and tissue compartments were 12 and 11%, respectively. The drug was shown, by equilibrium dialysis method, to be highly bound to plasma proteins (greater than 75%) within the usual therapeutic range (50 to 150 mug/ml) of plasma levels. The systemic availability of sulphadimethoxine from the oral suspension was 32.8% (22.5-80.0). Since the absorption half time, 1.87 (0.86-3.22) hours, was considerably shorter than the half-life, 13.10 (9.71-16.50) hours, of the drug, the rate of absorption would have little influence on the dosage regimen. Based on the experimental data obtained, a satisfactory dosage regimen might consist of a priming dose of 55 mg/kg by the intravenous route and maintenance doses of either 27.5 mg/kg of sulphadimethoxine injection given intravenously or 55 mg/kg of the oral suspension administered at 24 hour intervals. The adequacy and duration of therapy will depend upon the clinical response obtained.  (+info)

(3/81452) Specific receptors for glucocorticoid in the cytoplasm of the liver of AH 130 tumor-bearing rats.

Specific receptors for dexamethasone (11beta, 17alpha, 21-trihydroxy-9alpha-fluoro-16alpha-methyl-1,4-pregnadiene-3,20-dione) in the cytoplasm of the liver from AH 130 (solid type) tumor-bearing rats markedly increased in the advanced stage of tumor growth. The cytoplasmic receptors of the livers of normal and tumor-bearing rats differed in their affinities for dexamethasone, and their apparent equilibrium (dissociation) constants (K) for dexamethasone were 4.0 and 2.6 X 10(-9) M, respectively. The rates of dissociation of dexamethasone-receptor complexes and the heat denaturations of the receptors in the livers of normal and tumor-bearing rats were similar. The glucocorticoid receptors of tumor-bearing rat liver had slightly higher affinities than did those of normal liver for all the steroids tested. Only a trace amount of receptors for dexamethasone could be detected in the cytoplasm of AH 130 ascites cells.  (+info)

(4/81452) The interaction of rhodium(II) carboxylates with enzymes.

The effect of rhodium(II) acetate, propionate, and methoxyacetate on the activity of 17 enzymes was evaluated. The enzymes were preincubated with the rhodium(II) complexes in order to detect irreversible inhibition. All enzymes that have essential sulfhydryl groups in or near their active site were found to be irreversibly inhibited. Those enzymes without essential sulfhydryl groups were not affected. In each case, the rate of inactivation closely paralleled the observed toxicity and antitumor activity of rhodium(II) carboxylates; that is, rhodium(II) propionate greater than rhodium(II) acetate greater than rhodium(II) methoxyacetate. In addition, those enzymes that have been demonstrated to be most sensitive to established sulfhydryl inhibitors, such as glyceraldehyde-3-phosphate dehydrogenase, were also most sensitive to rhodium(II) carboxylate inactivation. Proton nuclear magnetic resonance measurements made during the titration of rhodium(II) acetate with cysteine showed that breakdown of the carboxylate cage occurred as a result of reaction with this sulfhydryl-containing amino acid.  (+info)

(5/81452) The direct spectrophotometric observation of benzo(a)pyrene phenol formation by liver microsomes.

Optical spectral repetitive scan analysis during the oxidative metabolism of benzo(a)pyrene by liver microsomal suspensions reveals the time-dependent formation of an intermediate(s) of which the visible spectra resemble those of several benzo(a)pyrene phenols. Liver microsomes from 3-methylcholanthrene-treated rats showed a greater rate of formation of the phenols than did microsomes from control animals; the rate of formation catalyzed by liver microsomes from phenobarbital-pretreated rats was intermediate. When 3-hydroxybenzo(a)pyrene was used as a standard for comparison of activity, the rates of formation of phenols were compared when measured by fluorometric, spectrophotometric, or high-pressure liquid chromatographic analytical techniques. An epoxide hydrase inhibitor, 1,1,1-trichloropropene-2,3-oxide, enhanced phenol formation regardless of the source of liver microsomes, and 7,8-benzoflavone inhibited control and 3-methylcholanthrene-induced microsomal metabolism of benzo(a)pyrene, 7,8-Benzoflavone did not effect benzo(a)pyrene metabolism by liver microsomes from phenobarbital-pretreated rats. The effect of inhibitors on the spectrophotometric assay correlates well with the results obtained from benzo(a)pyrene metabolite analysis using high-pressure liquid chromatography.  (+info)

(6/81452) Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells.

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

(7/81452) Action of partially thiolated polynucleotides on the DNA polymerase alpha from regenerating rat liver.

The effects of partially thiolated polynucleotides on the DNA polymerase alpha from regenerating rat liver were investigated. The enzyme was isolated from the nuclear fraction essentially according to the method of Baril et al.; it was characterized as the alpha polymerase on the basis of its response to synthetic templates and its inhibition with N-ethylmaleimide. Although polycytidylic acid had no effect on the DNA polymerase alpha either as a template or as an inhibitor, partially thiolated polycytidylic acid (MPC) was found to be a potent inhibitor, its activity being directly related to its extent of thiolation (percentage of 5-mercaptocytidylate units in the polymer). In comparison, the DNA polymerase beta which was purified from normal rat liver nuclear fraction, was much less sensitive to inhibition by MPC. Analysis of the inhibition of the alpha polymerase by the method of Lineweaver and Burk showed that the inhibitory action of MPC was competitively reversible with the DNA template, but the binding of the 7.2%-thiolated MPC to the enzyme was much stronger than that of the template (Ki/Km less than 0.03). Polyuridylic acid as such showed some inhibitory activity which increased on partial thiolation, but the 8.4%-thiolated polyuridylic acid was less active than the 7.2% MPC. When MPC was annealed with polyinosinic acid, it lost 80% of its inhibitory activity in the double-stranded configuration. However, 1 to 2%-thiolated DNA isolates were significantly more potent inhibitors than were comparable (1.2%-thiolated) MPC and showed competitive reversibility with the unmodified (but "activated") DNA template. These results indicate that the inhibitory activities of partially thiolated polynucleotides depend not only on the percentage of 5-mercapto groups but also on the configuration, base composition, and other specific structural properties.  (+info)

(8/81452) The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture.

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)


The renal threshold for glucose is 3 milligrams per millilitre of plasma. If the concentration of glucose in?


in the plasma exceeds this value, 
A) glucose will be excreted in the urine. 
B) glucose transporters in the proximal tubule will become saturated. 
C) penicillin will compete for binding sites on the glucose transporters in the proximal tubule. 
D) A & B 
E) A & C
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A !


which of the following are true about competitive antagonists?


1) they have an intrinsic activity of 0
2) they have an affinity for the agonist binding site on the receptor
3) they shift the apparent EC50 of an agonist to the right
4) the effect of a competitive antagonist can be overcome by adding more agonist.
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they are all true.-
1) all antagonists have intrinsic activity of 0.-
2) the bind the same place the agonist, that's why they are competitive.-
3) the curve moves to the right cause the sites occupied by the antagonist won't respond, and that way it'll look as the agonist has less effect.-
4) competitive antagonist bind in a reversible way, so if you add more agonist , it'll move the antagonist and bind with the receptors.-
hope i made myself clear, if not, tell me and i'll try to explain it in some other way.-
:)


Are phyto-estrogens harmful if someone is already estrogen-dominant?


I've heard conflicting ideas on this one. I know they can be beneficial is someones lifestyle is high in xenoestrogens, as they occupy the binding sites so harmful xenoestrogens cant bind. But if someone eats organic and lives a natural lifestyle, are phytoestrogens in plants harmful? Can they cause the estrogen-dominance to become worse?
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No I don't think so because phyto-estrogens can bind to both the estrogen receptor and the progesterone receptor so should not cause an increase in the estrogen/progesterone ratio or in other words should not cause estrogen dominance. They are thought to have a stabilising effect where there might be an excess of estrogen for instance. 

But I would be careful of having an excess of soy estrogens because they are said to contain endocrine disrupting agents that can have a detrimental effect on the thyroid gland. This could especially be harmful in someone who has a low thyroid function. Other phyto-estrogens are fine in this respect as far as I am aware.

Agnus castus (chaste berry) is a herb you can take to help improve the progesterone/estrogen ratio, thus reducing an estrogen dominance. It is said to exert its effect at the hypothalamus and pituitary gland function level. It is believed to modulate the levels of gonadotropin releasing hormone (GnRH) and follicle stimulating hormone releasing hormone (FSH) to normalise the secretion of the hormones prolactin (reduces this) and the estrogen/progesterone ratio (reduces this). Thus progesterone levels should normalise. This may take a while but persevere. However, you must not take this herb if you are pregnant or lactating or on the birth control pill. 
If you are on any other medication - talk to your doctor or pharmacist first. 

Hope this helps.
Best wishes.


What are good sites to find free make-up samples?


I also need those sites that can deliver to the Philippines. I've found a few sites that give this but sadly they're only few =(
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i dun have sites that give you samples, but i do have one site that have competitions and you can easily win a whole make up product not sample but im not sure if they ship it to philippines. hope it might help you a bit... 
rimmel - http://www.rimmellondon.com/RimmelLondon/


What are the sites for parenting advice and children magazines?


I need the actual web sites' addresses. I've tried to perform my own search; however, it only gave me offers for free subscriptions. I need it for work purposes. I am a Toddler Teacher. Thanks!
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Parents Magazine
http://www.parents.com/

National Geographic Kids
http://kids.nationalgeographic.com/

Cookie
http://www.cookiemag.com/

Highlights
http://www.highlights.com/

This site is still available though the magazine is no longer being published
Wondertime
http://wondertime.go.com/


Hope this helps


What sites are good for making a free personal workout plan?


What sites can i go to make a free personal work out plan. I want to be able to put down what i want to work on and it can tell me what i will need to do at the gym. THANKS
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There are tons of web sites and magazines and books. Just do some research. You are only going to get spammed to death asking that question here.


What internet sites are blocked at your school?


I'm at school at the moment and this is really the only bareable site that you can go on.

My Q is, What country are you from (I'm from Australia), and does your school have blocked sites?  We can't even get on to google images.
I can't get onto Facebook, twitter, youtube or anything like that either.  I can go onto Wikipedia though.
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Nothings blocked, but everything is very supervised. Theres a main computer in each computer room where the teacher can see and record everything, so if they see your on any site that's not school related they get pissed.
I'm from Canada:)


What online tattoo design site offers a monthly fee for access to tattoo designs including stenciles?


Any good online sites for tattoo designs with stenciles that offer a month fee instead of a per design fee?. Thanks
I'm starting to do tattoos so I'm building the essentials to begin. Any website names would be appreciated. I have found a few, but I know many in the business have a particular site(s) they like.
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I have used this site: http://www.create-your-tattoo.com/ several times now.  It's a one time year or lifetime payment and totally worth it!  They even have a money back guarantee.  They have 1000's of designs and you can even combine designs and the galleries are amazing.  Check it out!