Batch Cell Culture Techniques
Culture Techniques
Culture Media
Evidence of exponential growth of an anammox population in an anaerobic batch culture. (1/89)
Twenty-five replicates of growth medium for anaerobic ammonium oxidation (anammox) containing (15)N-labeled ammonium and non-labeled nitrite were inoculated into an anammox enrichment culture at low density, and anaerobically incubated batchwise. In the headspace, (29)N(2) partial pressure linearly increased via anammox in 25 vials, confirming that anammox populations were viable in all subcultures. On prolonged incubation, exponential increases in (29)N(2) were not observed in all but 13 subcultures, suggesting that the anammox population may not proliferate unless all conditions for growth are satisfied. The estimated first-order rate coefficients in those 13 subcultures varied from 0.0029 to 0.0048 h(-1). (+info)A novel recirculating flow-perfusion bioreactor for periosteal chondrogenesis. (2/89)
(+info)Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains. (3/89)
(+info)Physico-chemical characteristics and functional properties of chitin and chitosan produced by Mucor circinelloides using yam bean as substrate. (4/89)
(+info)Evaluation of a laboratory-scale bioreactive in situ sediment cap for the treatment of organic contaminants. (5/89)
(+info)Optimisation of surface expression using the AIDA autotransporter. (6/89)
(+info)Parametric optimization of feruloyl esterase production from Aspergillus terreus strain GA2 isolated from tropical agro-ecosystems cultivating sweet sorghum. (7/89)
A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71- 0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of 30 degrees C. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions. (+info)Repeated-batch operation of immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor for lactose hydrolysis. (8/89)
In this study, we investigated the performance of an immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor, where the cells were immobilized in alginate beads, which were then used in repeated-batch operations for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose over the long-term. In particular, in the Tris buffer system, disintegration of the alginate beads was not observed during the operation, which was observed for the phosphate buffer system. The o-nitrophenyl-beta-D-galactoside hydrolysis was operated successfully up to about 80 h, and the runs were successfully repeated at least eight times. In addition, hydrolysis of lactose was successfully carried out up to 240 h. Using Western blotting analyses, it was verified that the beta-galactosidase inclusion bodies were sustained in the alginate beads during the repeated-batch operations. Consequently, we experimentally verified that beta-galactosidase inclusion bodies-containing Escherichia coli cells could be used in a repeated-batch reactor as a biocatalyst for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose. It is probable that this approach can be applied to enzymatic synthesis reactions for other biotechnology applications, particularly reactions that require long-term and stable operation. (+info)Batch cell culture techniques refer to a method of growing cells in which all the necessary nutrients are added to the culture medium at the beginning of the growth period. The cells are allowed to grow and multiply until they exhaust the available nutrients, after which the culture is discarded. This technique is relatively simple and inexpensive but lacks the ability to continuously produce cells over an extended period.
In batch cell culture, cells are grown in a closed system with a fixed volume of medium, and no additional nutrients or fresh medium are added during the growth phase. The cells consume the available nutrients as they grow, leading to a decrease in pH, accumulation of waste products, and depletion of essential factors required for cell growth. As a result, the cells eventually stop growing and enter a stationary phase, after which they begin to die due to lack of nutrients and buildup of toxic metabolites.
Batch cell culture techniques are commonly used in research settings where large quantities of cells are needed for experiments or analysis. However, this method is not suitable for the production of therapeutic proteins or other biologics that require continuous cell growth and protein production over an extended period. For these applications, more complex culture methods such as fed-batch or perfusion culture techniques are used.
Cell culture is a technique used in scientific research to grow and maintain cells from plants, animals, or humans in a controlled environment outside of their original organism. This environment typically consists of a sterile container called a cell culture flask or plate, and a nutrient-rich liquid medium that provides the necessary components for the cells' growth and survival, such as amino acids, vitamins, minerals, and hormones.
There are several different types of cell culture techniques used in research, including:
1. Adherent cell culture: In this technique, cells are grown on a flat surface, such as the bottom of a tissue culture dish or flask. The cells attach to the surface and spread out, forming a monolayer that can be observed and manipulated under a microscope.
2. Suspension cell culture: In suspension culture, cells are grown in liquid medium without any attachment to a solid surface. These cells remain suspended in the medium and can be agitated or mixed to ensure even distribution of nutrients.
3. Organoid culture: Organoids are three-dimensional structures that resemble miniature organs and are grown from stem cells or other progenitor cells. They can be used to study organ development, disease processes, and drug responses.
4. Co-culture: In co-culture, two or more different types of cells are grown together in the same culture dish or flask. This technique is used to study cell-cell interactions and communication.
5. Conditioned medium culture: In this technique, cells are grown in a medium that has been conditioned by previous cultures of other cells. The conditioned medium contains factors secreted by the previous cells that can influence the growth and behavior of the new cells.
Cell culture techniques are widely used in biomedical research to study cellular processes, develop drugs, test toxicity, and investigate disease mechanisms. However, it is important to note that cell cultures may not always accurately represent the behavior of cells in a living organism, and results from cell culture experiments should be validated using other methods.
Culture techniques are methods used in microbiology to grow and multiply microorganisms, such as bacteria, fungi, or viruses, in a controlled laboratory environment. These techniques allow for the isolation, identification, and study of specific microorganisms, which is essential for diagnostic purposes, research, and development of medical treatments.
The most common culture technique involves inoculating a sterile growth medium with a sample suspected to contain microorganisms. The growth medium can be solid or liquid and contains nutrients that support the growth of the microorganisms. Common solid growth media include agar plates, while liquid growth media are used for broth cultures.
Once inoculated, the growth medium is incubated at a temperature that favors the growth of the microorganisms being studied. During incubation, the microorganisms multiply and form visible colonies on the solid growth medium or turbid growth in the liquid growth medium. The size, shape, color, and other characteristics of the colonies can provide important clues about the identity of the microorganism.
Other culture techniques include selective and differential media, which are designed to inhibit the growth of certain types of microorganisms while promoting the growth of others, allowing for the isolation and identification of specific pathogens. Enrichment cultures involve adding specific nutrients or factors to a sample to promote the growth of a particular type of microorganism.
Overall, culture techniques are essential tools in microbiology and play a critical role in medical diagnostics, research, and public health.
Bacteriological techniques refer to the various methods and procedures used in the laboratory for the cultivation, identification, and study of bacteria. These techniques are essential in fields such as medicine, biotechnology, and research. Here are some common bacteriological techniques:
1. **Sterilization**: This is a process that eliminates or kills all forms of life, including bacteria, viruses, fungi, and spores. Common sterilization methods include autoclaving (using steam under pressure), dry heat (in an oven), chemical sterilants, and radiation.
2. **Aseptic Technique**: This refers to practices used to prevent contamination of sterile materials or environments with microorganisms. It includes the use of sterile equipment, gloves, and lab coats, as well as techniques such as flaming, alcohol swabbing, and using aseptic transfer devices.
3. **Media Preparation**: This involves the preparation of nutrient-rich substances that support bacterial growth. There are various types of media, including solid (agar), liquid (broth), and semi-solid (e.g., stab agar). The choice of medium depends on the type of bacteria being cultured and the purpose of the investigation.
4. **Inoculation**: This is the process of introducing a bacterial culture into a medium. It can be done using a loop, swab, or needle. The inoculum should be taken from a pure culture to avoid contamination.
5. **Incubation**: After inoculation, the bacteria are allowed to grow under controlled conditions of temperature, humidity, and atmospheric composition. This process is called incubation.
6. **Staining and Microscopy**: Bacteria are too small to be seen with the naked eye. Therefore, they need to be stained and observed under a microscope. Gram staining is a common method used to differentiate between two major groups of bacteria based on their cell wall composition.
7. **Biochemical Tests**: These are tests used to identify specific bacterial species based on their biochemical characteristics, such as their ability to ferment certain sugars, produce particular enzymes, or resist certain antibiotics.
8. **Molecular Techniques**: Advanced techniques like PCR and DNA sequencing can provide more precise identification of bacteria. They can also be used for genetic analysis and epidemiological studies.
Remember, handling microorganisms requires careful attention to biosafety procedures to prevent accidental infection or environmental contamination.
Culture media is a substance that is used to support the growth of microorganisms or cells in an artificial environment, such as a petri dish or test tube. It typically contains nutrients and other factors that are necessary for the growth and survival of the organisms being cultured. There are many different types of culture media, each with its own specific formulation and intended use. Some common examples include blood agar, which is used to culture bacteria; Sabouraud dextrose agar, which is used to culture fungi; and Eagle's minimum essential medium, which is used to culture animal cells.
Penicillin
Montrose Thomas Burrows
Fed-batch culture
List of animals that have been cloned
CASY cell counting technology
Microbial cell factory
Intestine-on-a-chip
Metabolic flux analysis
Cell counting
Xanthomonas campestris
Cultured meat
Virtual colony count
Reverse transfection
Viral plaque
Bokashi (horticulture)
Streptomycin
Julius Youngner
Monoclonal antibody
Zetaproteobacteria
Affimer
Pruno
Brocadia
Industrial enzymes
Stuck fermentation
Cellular agriculture
Cell sorting
Kantai Collection
Human betaherpesvirus 5
Immunoperoxidase
Vincristine
Neural network
Batch Cell Culture Techniques | Profiles RNS
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Bioreactor4
- Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)
- The bioreactor then continuously suffuses the cultured cells with concentrations of nutrients and hormones that mimic the body's natural state. (vumc.org)
- We can offer static flask, spinner flask and hollow fiber bioreactor cultures to meet your needs from 10 mg to 10 g. (iqproducts.nl)
- When culture volume and cell density are optimal, the culture is then transferred to a production bioreactor. (sigmaaldrich.com)
Fetal bovin4
- Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)
- Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS). (sigmaaldrich.com)
- Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
- iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
Mammalian cell culture3
- Figure 1: Comparison of analyzers with respect to their ease of implementation in microbial fermentations and mammalian cell culture unit operations for various quality attributes: (a) misincorporation, (b) nutrients, (c) glycosylation, and (d) cell growth. (biopharminternational.com)
- Mammalian cell culture reactors were adapted from chemical mixing tanks, which used shaft-and-impeller mixing. (genengnews.com)
- Knowledge of basic immunology and cell biology techniques, including mammalian cell culture, is required. (affyimmune.com)
Therapies11
- Unlike whole cell therapies, EVs are acellular products, the equivalent of text messages sent back and forth in order to elicit reactions. (corning.com)
- Their small size confers other benefits, as well - too small to convey pathogens such as bacteria or viruses, EVs are also incapable of the unwanted replication or tumor formation that whole cell therapies can occasionally provoke. (corning.com)
- The potential EVs possess for the field of acellular therapies - treatments derived from acellular products that achieve the same therapeutic response without delivering entire cells - is an exciting one. (corning.com)
- Appia Bio is a preclinical stage biotech company founded in 2020 with the mission to bring innovative off-the-shelf cell therapies to cancer patients. (berkeley.edu)
- Our goal is to discover and develop allogeneic cell therapies across a broad array of cancer indications with a scalability that increases access for patients. (berkeley.edu)
- The consortium specifically focuses on the manufacturing of protein therapies like antibodies, gene therapies, cell therapies, and vaccines. (unc.edu)
- Quell Therapeutics Ltd ("Quell"), the world leader in the development of engineered T-regulatory (Treg) cell therapies for serious medical conditions driven by the immune system, announced it has raised $156 million in an oversubscribed Series B financing. (bios.community)
- In recent years, cell therapies and other Advanced Therapy Medicinal Products (ATMPs) have begun to come into their own as highly promising treatments for a wide range of serious, chronic and rare diseases. (amerisourcebergen.com)
- With a record number of new cell and gene therapies (CGTs) approved in 2021( 1 ) , a surge in commercializations is anticipated. (amerisourcebergen.com)
- During a recent roundtable hosted by The Medicine Maker, Andrea Zobel, Senior Director, Personalized Supply Chain at World Courier, explored the complexities of bringing cell therapies to the market. (amerisourcebergen.com)
- There are a few transport obstacles that need to be overcome to ensure successful commercialization of cell therapies. (amerisourcebergen.com)
Nutrients6
- Batch cell culture technique where the culture is fed with nutrients throughout the process, instead of all at once in the beginning. (nih.gov)
- The cells in the kidney's tubule grow easily in culture, and use chemical energy derived from the body's nutrients to do the concentrating, so our implantable artificial kidney does not need to rely on electrical power and pumps. (vumc.org)
- This phenomenon is known as "cell culture stress," and it happens because cells cannot be continuously supplied with fresh nutrients and oxygen like they are in the body. (vumc.org)
- In the organism, liposoluble nutrients such as cholesterol, fatty acids and lipophilic vitamins reach the cells by means of specific apolipoproteins which form complexes with the lipids, the so-called lipoproteins (LPs), and which recognize specific membrane receptors expressed by the cells. (forschung3r.ch)
- The device can be used to rapidly monitor culture media for nutrients and supplements to improve product yield and quality. (unc.edu)
- Because cell lines are unique and require different nutrients to thrive, culture biomass and protein production are largely dependent on the culture media used. (sigmaaldrich.com)
Bioreactors5
- PBS' U-shaped bioreactors employ a patented mixing technology, the Air-Wheel™, that exploits the fact that cell cultures must be oxygenated. (genengnews.com)
- To support the development of monoclonal antibodies, manufacturers have constructed sizable manufacturing facilities with several cell culture bioreactors. (researchnester.com)
- Bioreactors are large scale culture devices used in upstream processes and production that play a key role in expanding and scaling cell culture for production. (sigmaaldrich.com)
- The typical seed train cell expansion process involves thawing cryopreserved cells followed by successive expansions into larger culture vessels including flasks, spinners, and stirred bioreactors. (sigmaaldrich.com)
- Bioreactors provide controlled microenvironments and nutrient delivery to regulate cell growth and differentiation, improving standardization and reproducibility. (sigmaaldrich.com)
Clinical5
- PBS offers scalability from three liters for lab work, and 80 liters for preclinical and clinical batches. (genengnews.com)
- AffyImmune Therapeutics ("Affy"), a clinical stage biotechnology company focused on developing technologies that expand the utility and improve the safety and efficacy of cell-based immunotherapy for cancer, is seeking an Analytical Development Scientist to help advance the use of CAR T therapy for solid tumors. (affyimmune.com)
- Assist in development of clinical manufacturing documents (IND amendments, master batch records, etc. (affyimmune.com)
- However, to facilitate their clinical use and to ensure safety, iPS culturing protocols must be compliant with good manufacturing practice guidelines and devoid of xenogenic products. (biomedcentral.com)
- This is a multi-disciplinary approach that integrates molecular, biochemical and clinical techniques with developmental and engineering processes. (openorthopaedicsjournal.com)
Hematopoietic stem1
- Our research programs are rooted in leveraging T cell biology to generate NKT and T cell subtypes from hematopoietic stem cells. (berkeley.edu)
Perfusion culture1
- The production of monoclonal antibodies is being carried out on a wide scale employing group, ongoing, and perfusion culture techniques. (researchnester.com)
Methods6
- Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest. (rush.edu)
- Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
- These growth strategies, together with optimization of media composition and the application of molecular biology methods, made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation. (pfeiffertheface.com)
- Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
- In the present work, a combination of two methods was tested: FACS combined with growing cells in 96-well plates containing solid agar growth medium to accelerate both the isolation procedure and culture scale-up. (biomedcentral.com)
- METHODS: HEK293 (human epithelial kidney) and swine testis (ST) cells were co-cultured with macroencapsulated pig islets embedded in an alginate patch, macroencapsulated PK15 (swine kidney epithelial) cells embedded in an alginate patch and free PK15 cells. (bvsalud.org)
Tissues5
- Extracellular vesicles act as a cell's transfer signals - these nanoparticles, which contain protein, sugars, and other biological materials, communicate with adjacent cells and tissues to transmit messages, such as whether a cell is healthy or in distress. (corning.com)
- The implantable bioartificial kidney combines nano-technology with tissues from human kidney cells. (vumc.org)
- 1) One is to replace serum by other biological but as yet inadequately defined mixtures such as extracts from plants, animal tissues or cells. (forschung3r.ch)
- Tissues and Cells. (lu.se)
- In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
Assays4
- 4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)
- In addition, batch culture growth kinetics assays will be crucial to learning the cellular and molecular mechanisms responsible for susceptibility and resistance to EIPE-1. (okstate.edu)
- Prior experience with the design, execution, optimization, and validation of a broad range of in vitro cell based assays is required. (affyimmune.com)
- Experienced and well-versed with FDA Guidance related to CAR T cell therapy manufacturing and release testing and industry whitepapers for state-of-the-art method validation of manufacturing assays. (affyimmune.com)
Immunology and cell1
- Detailed understanding of immunology and cell therapy. (bios.community)
Membranes2
- and we will begin manufacturing larger batches of the membranes for testing in the full-scale cartridges. (vumc.org)
- It is an important building block of all cell membranes, and an important metabolite in many biosynthetic pathways. (forschung3r.ch)
Vitro cell1
- The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
Densities1
- The standard procedure for growing E. coli cells to high cell densities is the fed-batch technique, where the carbon substrate, e.g. glucose, controls the growth as a limiting factor. (pfeiffertheface.com)
Embryonic3
- This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
- Several mammalian cell lines, including CHO, lymphoma (NS0, SP2/0) and human embryonic kidney (HEK) cells, have been developed and used to produce therapeutic proteins and antibodies at high levels. (sigmaaldrich.com)
- Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
Formulation3
- Emerging market trends include improvements in the creation and formulation of cell culture medium. (researchnester.com)
- Demonstrable expertise in cell-based techniques including cell enrichment, culture, and formulation technologies. (bios.community)
- An important aspect is to look at the raw materials used in the formulation of the cells and how they are packaged up in that formulation. (amerisourcebergen.com)
20241
- Cell Culture Consumables Market size is set to cross USD 400 billion by the end of 2036, expanding around 23% CAGR during the forecast period i.e., 2024 - 2036. (researchnester.com)
Vaccine1
- The FDA has issued a draft guideline on new cell substrates for vaccine manufacturing to detail requirements in this area. (gmp-compliance.org)
Microbial cells1
- Biotherapeutics are produced using genetically modified, live mammalian and microbial cells and require cell culture media for their development. (unc.edu)
Grown8
- Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
- In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
- This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
- We are now ready and equipped to accelerate the development of our products and make a true innovative impact on the way cells are grown. (greenqueen.com.hk)
- Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. (illinois.edu)
- The design features an artificial filtration unit to replace the cells damaged in end-stage renal failure, because functional filtration cells are not feasibly grown in culture. (vumc.org)
- Cells grown in culture simply do not function the same as cells taken from human kidneys, a feature common to almost all stem cells. (vumc.org)
- The cells are grown in a specialized framework that supplies them with critical growth factors so that scientists can observe how gravity affects growth and specialization as cells become new lung tissue. (nasa.gov)
Escherichia1
- What is Escherichia coli in urine culture? (pfeiffertheface.com)
Adherent5
- There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
- While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
- There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
- The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
- Adherent cultures can be further divided into 2D and 3D cultures. (integra-biosciences.com)
Life Sciences1
- According to Christian Kaisermayer, Ph.D., project manager for cell culture at GE Healthcare Life Sciences the two significant drivers are process flexibility and complete solutions. (genengnews.com)
Infectious diseases2
- 1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)
- IQ Products is a manufacturer of high quality, ready-to-use antibodies and antibody combinations for the detection of Human Leukocyte Antigens, Human MHC Antigens, Human Cytokines, Human Fetal Erythrocytes, Apoptosis, T cell responses and infectious diseases. (iqproducts.nl)
Continuous3
- The cells can't adhere to the walls due to the continuous stirring, so spheroids are formed. (integra-biosciences.com)
- Unlike traditional batch technologies in the sector, the startup has developed a continuous cell culture system that enables high yields at greater cost efficiencies, which has opportunities for large scale applications in the cultivated meat industry. (greenqueen.com.hk)
- Their novel approach, which is a continuous cell culturing system, enables higher yields with lower input requirements, while also lowering the environmental footprint of manufacturing compared to traditional batch technologies. (greenqueen.com.hk)
Reproducibility1
- We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
Biology5
- It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
- For the quality assurance you need for molecular biology and cell culture, Corning ® Costar ® Stripette ® or KIMAX ® 51 serological pipettes are hard to beat. (sigmaaldrich.com)
- Advanced degree (PhD, PharmD, or MS) and human T cell biology experience required. (affyimmune.com)
- Primary neuronal culture is the possibility to visually identify neurons, characterize in details both action potentials and underlying ionic currents and to correlate electrophysiology and molecular biology on the same batch of neurons. (sav.sk)
- BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Bacterial3
- These unusual findings suggest that this method for abrupt change in growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell. (fit.edu)
- Although bacterial, yeast, and insect cell expressions systems are capable of overexpressing recombinant proteins, mammalian cells are most commonly used for biologics production due to their ability to propagate human viruses, express monoclonal antibodies, and incorporate post-translational modifications such as glycosylation that are critical to the production of effective biologics. (sigmaaldrich.com)
- Ammonium sulphate and ammonium nitrate at a concentration of 1 g/L served as the best nitrogen sources for bacterial growth, allowing B. subtilis and E. coli cells to accumulate PHA up to 62.21 and 58.7%, respectively. (scielo.org)
Cultivation4
- High cell density cultivation (HCDC) is developed for the production of microbial biomasses and their products. (pfeiffertheface.com)
- What is high cell density cultivation? (pfeiffertheface.com)
- High-cell-density cultivation (HCDC) is required to improve microbial biomass and product formation substantially. (pfeiffertheface.com)
- On the subject of integration and "complete solutions," GE Healthcare's ReadyToProcess™ product line covers all unit operations from media preparation and cell cultivation to protein purification. (genengnews.com)
Coli cells1
- What will help to achieve high density of E coli cells? (pfeiffertheface.com)
Apoptosis1
- Advances in gene editing such as CRISPR-Cas systems further enhance production efficiency and improve product quality through modification of host cell genomes to manipulate cell growth and apoptosis, and to promote post-translational modification of the gene product. (sigmaaldrich.com)
Biological2
- Mast cells play an important role in a variety of biological processes and actively participate in the inflammatory response. (bvsalud.org)
- Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)
Protein purification1
- Prior to joining Sartorius, in 2001, he began his involvement with the identification and development of integrated automated systems in cell culture, protein purification and vial filling including the Ambr® products. (b3cnewswire.com)
Mimic1
- Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
Gene1
- Cell and Gene Therapy is a technique that modifies a person's genes to treat or cure disease. (ncbionetwork.org)
Serum8
- Depending on the species of the serum, each batch of serum is tested for adventitious viruses using cell culture techniques. (biowest.net)
- In his present investigation he used this serum-free aggregating brain cell cultures as a reference culture system. (forschung3r.ch)
- To avoid the use of serum in culture media, two approaches are currently being employed. (forschung3r.ch)
- Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
- The further identification of serum components that are obviously required by cultured cells is of less high priority. (forschung3r.ch)
- It appears reasonable to assume that these particular serum components would be required by all cell types. (forschung3r.ch)
- Knowing that lipids, and particularly cholesterol, are absent from most conventional culture media, the working hypothesis of the present investigation was that the LPs represented the still undefined serum component(s) required for cell culture. (forschung3r.ch)
- For instance, the batch-to-batch variability has decreased as a result of the application of serum-free and chemically controlled media for bioproduction. (researchnester.com)
Variability1
- For example, in humans variability in cell density among cells of a given cell type is 100 times smaller than variation in cell mass. (pfeiffertheface.com)
Stem7
- The one that has arguably received the most attention from industry are what have been referred to as "naïve" EVs, or unmodified EVs produced from stem cells. (corning.com)
- 7. What about stem cells? (vumc.org)
- Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. (biomedcentral.com)
- Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
- The most commonly used type of stem cells, mesenchymal stem cells (MSCs), can be easily isolated from bone marrow or synovium and cultured in vitro . (openorthopaedicsjournal.com)
- Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
- Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
Biologics3
- Aside from cell-based meat, the company's technology can be applied in the cell therapy, pharmaceutical, medical and biologics fields, but co-founders Martina Miotto, Che Connon and Leo Groenewegen believe that cultured meat is where the real opportunity for growth lies. (greenqueen.com.hk)
- Cultured cells are used for the manufacturing of biologics such as vaccines, therapeutic proteins and antibodies, and cell therapy products. (sigmaaldrich.com)
- Culture medium optimization is another critical component in the development of cell culture processes for the manufacturing of biologics. (sigmaaldrich.com)
Vaccines2
- The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. (sigmaaldrich.com)
- Her chief research interest is the development of viral-vectored vaccines that work by inducing strong and protective T and B cell responses. (isv-online.org)
Immunological1
- Immunological techniques: Immunodiffusion, immunoelectrophoresis, RIA and ELISA. (entrance-exam.net)
Analytical techniques3
- Although the term analytical in PAT is broadly defined to include chemical, physical, microbiological, mathematical, and risk analysis conducted in an integrated manner (see Table I), the emphasis in this article is on analytical techniques that enable the monitoring of critical performance attributes of raw and in-process materials and processes during biotechnology manufacturing. (biopharminternational.com)
- However, it is important to understand that the goal of PAT is not only the use of these analytical techniques for monitoring, but also to control the manufacturing process to consistently yield the desired product quality. (biopharminternational.com)
- We're trying introduce analytical techniques that are faster and can be done inline or at-line during manufacturing - decreasing manufacturing time and increasing the rate of product release," Carbonell says. (unc.edu)
Growth9
- Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
- The bioassay was performed by dissolving the compounds in dimethyl sulfoxide and impregnating filter paper disks which were placed onto Mueller Hinton agar plates spread inoculated in a standardized manner to obtain even cell lawns after incubation for 18±1 hours at 37°C. Zones of growth inhibition were measured with the aid of electronic calipers. (okstate.edu)
- Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
- Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)
- Numerous improvements have been made in the production and growth of cell culture medium. (researchnester.com)
- A collection of experiment cassettes is used to accommodate experiments in cell technology, multiphase fluids, solution chemistry, separation science, microencapsulation, and crystal growth. (nasa.gov)
- Also know asexual reproduction: Budding-a new organism develops from an outgrowth or bud on another one.Examples: Hydra Binary fission-Cell splits and produces two separate cells.Examples: Bacteria Regeneration is the process of renewal, restoration, and growth. (gnfchurch.net)
- Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. (biomedcentral.com)
- Research has shown the importance of growth factors in guiding and modulating the differentiation of MSCs in order to obtain the required cell type. (openorthopaedicsjournal.com)
Process8
- Appia Bio is seeking a motivated Sr. Research Associate or Research Associate II to be part of the Process Design team with previous cell culture experience. (berkeley.edu)
- They will be working in a highly collaborative environment to develop an innovative process for a manufacturable cell therapy product. (berkeley.edu)
- Cell culture refers to the process of growing cells under controlled conditions outside their natural environment. (integra-biosciences.com)
- Decreases in TraR activity and conjugative competence could be accounted for by dilution associated with cell division, suggesting that while induction of Ti plasmid conjugation is an active process, the cells lack a mechanism for disassembling the conjugative apparatus when signals become limiting. (illinois.edu)
- In the following subsections, we briefly review some of the commonly used process analysis techniques in the biotech industry (5). (biopharminternational.com)
- We are looking to recruit a Senior/Principal Scientist Manufacturing Science & Technology to lead a team of cell process subject matter experts (SMEs), providing process stewardship, technical support and connectivity between process development and manufacturing sites. (bios.community)
- To gain pluripotency, the reprogramming process typically involves transfecting adult somatic cells with certain pluripotency markers. (biomedcentral.com)
- Commercializing a cell therapy is a complex process compared with other treatments, especially when it comes to supply chain needs. (amerisourcebergen.com)
Certifications1
- For titrations and analytical chemistry techniques, the BRAND ® BLAUBRAND ® ETERNA volumetric pipettes are supplied with original batch certifications. (sigmaaldrich.com)
Vivo3
- AffyImmune's Tune & Track platform finely tunes the affinity of CAR T cells to reduce toxicity and increase CAR T cell longevity while allowing in vivo monitoring through a proprietary tracking system. (affyimmune.com)
- Excitability will be investigated in primary culture of hippocampal neurons by patch clamp and in situ by in vivo electrophysiology. (sav.sk)
- In addition there is a lack of information regarding the presence of mast cells in dental pulp of rats, which are animals used to study important aspects of the pathophysiology of dental pulp in vivo 6 . (bvsalud.org)
Receptor1
- T cell receptor. (entrance-exam.net)
Concentration2
- The residual haemoglobin concentration in each batch is determined by a quantitative and colorimetric assay to verify that the proper collection and processing procedures have been followed. (biowest.net)
- Immobilization technique improved the concentration to 1350 μg/ml higher than that produced from free cells cultures and could be maintained for longer than 17 days in a repeated batch system. (scielo.org)
Mycoplasma3
- Each final product batch of sera are tested for Mycoplasma. (biowest.net)
- The sera are tested for the absence of Mycoplasma using a cell culture assay in Axcell Biotechnologies media by culture method. (biowest.net)
- Experience with analytical method development and validation for a variety of cell-based bioassays, including potency, mycoplasma, replication competent lentivirus, vector copy number, and sterility. (affyimmune.com)
Suspension3
- The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
- Forced-floating: a cell suspension is loaded into the wells of a low adhesion polymer-coated microplate. (integra-biosciences.com)
- Hanging drop: a cell suspension is loaded into the wells of a hanging drop plate. (integra-biosciences.com)
Antibody2
- We offer you conjugation of a volume as little as 1 mg of antibody up to large-scale single batches and the purification of the conjugates when necessary. (iqproducts.nl)
- In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells. (sigmaaldrich.com)
Synthetic1
- Because EVs are much smaller than whole cells, they are able to cross biologic barriers with ease, in contrast to the relative mass of whole cells or the immunogenic responses triggered by certain synthetic products. (corning.com)
Proteins2
- CellulaREvolution's technology is different from existing traditional batch-based cell culturing techniques, which are not able to meet the demand for cells as demand from various industries continues to grow - especially in the cultivated meat sector, where food techs are racing to launch their cell-based proteins on the market following the world's first regulatory approval for commercial sale in Singapore secured by Eat Just last year. (greenqueen.com.hk)
- Their proteins are properly folded, free from contaminating related TGF beta family proteins (unfortunately often present in mammalian protein expression systems) and they include full QC data for every batch - mass spec, analytical reverse phase, recovery, SDS-PAGE, bioassay data and endotoxin testing. (o2hventures.com)
Neurons1
- Fibrotic scar model and TGF-b1 differently modulate action potential firing and voltage-dependent ion currents in hippocampal neurons in primary culture. (sav.sk)
Immunogenic1
- These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
Agitation2
- It usually doesn't matter because agitation intensity can be adapted to the robustness of the cell line. (genengnews.com)
- According to Daniel Giroux, vp of R&D, duplicating the agitation mechanisms of small reactors in large systems is problematic without subjecting cells to significant shear damage. (genengnews.com)
Enables2
- Fluorescence activated cell sorting (FACS) enables the selection of particular strains of microalgae and subsequent isolation [ 16 ]. (biomedcentral.com)
- The characterization of different populations within any mixture of cells is performed through direct measurement of optical cell properties (for example, light scatter and multicolor fluorescence emission), which in turn enables FACS of defined cell populations that can be cultured separately at a later stage [ 17 - 19 ]. (biomedcentral.com)
Tissue5
- In order to reduce morbidity associated with STSG harvesting, researchers have developed autologous dermo-epidermal substitutes (DESs) using cell culture, tissue engineering, and, more recently, bioprinting approaches. (frontiersin.org)
- Using the latest bioengineering techniques, the Lung Tissue experiment cultures different types of lung cells in controlled conditions onboard the ISS. (nasa.gov)
- Newer techniques using tissue engineering to regenerate musculoskeletal tissue by using biomimetic materials are now being studied. (openorthopaedicsjournal.com)
- Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
- The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)
Responses1
- Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
Approaches1
- Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
Therapy4
- Experience working in cGMP development and/or cell therapy product manufacturing is highly desirable. (bios.community)
- With your analytical development, vendor management, and cell therapy expertise, you will leave an indelible mark on Affy. (affyimmune.com)
- Prior oncology, cell therapy, and/or CAR T experience preferred. (affyimmune.com)
- AffyImmune is realizing the potential of cancer immunotherapy by extending the anti-cancer activity of CAR T cell therapy to solid tumors. (affyimmune.com)
Biochemical1
- Biochemical separation techniques and characterization: ion exchange, size exclusion and affinity chromatography, electrophoresis, UV-visible, fluorescence and Mass spectrometry. (entrance-exam.net)
Hydrogel1
- Then, we constructed a fibrin hydrogel-based in vitro bioassay system for the simultaneous detection of skin permeability to and pro-inflammatory activity mediated by zinc ion released from NPs by using a stable reporter cell line and a fibrin hydrogel layer. (bvsalud.org)
Experimental2
- However, PERV was detected in 1 of the 3 experimental replicates of HEK293 cells cultured with encapsulated PK15 cells. (bvsalud.org)
- Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
Processes1
- Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes. (sigmaaldrich.com)
Stability1
- If you can really improve the stability of the cells post-thawing or in a fresh state, then these issues could be addressed, extending the shelf life of fresh batches and helping to alleviate the issues associated with cryostorage. (amerisourcebergen.com)