Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Methods for maintaining or growing CELLS in vitro.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Techniques used in studying bacteria.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

Evidence of exponential growth of an anammox population in an anaerobic batch culture. (1/89)

Twenty-five replicates of growth medium for anaerobic ammonium oxidation (anammox) containing (15)N-labeled ammonium and non-labeled nitrite were inoculated into an anammox enrichment culture at low density, and anaerobically incubated batchwise. In the headspace, (29)N(2) partial pressure linearly increased via anammox in 25 vials, confirming that anammox populations were viable in all subcultures. On prolonged incubation, exponential increases in (29)N(2) were not observed in all but 13 subcultures, suggesting that the anammox population may not proliferate unless all conditions for growth are satisfied. The estimated first-order rate coefficients in those 13 subcultures varied from 0.0029 to 0.0048 h(-1).  (+info)

A novel recirculating flow-perfusion bioreactor for periosteal chondrogenesis. (2/89)

 (+info)

Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains. (3/89)

 (+info)

Physico-chemical characteristics and functional properties of chitin and chitosan produced by Mucor circinelloides using yam bean as substrate. (4/89)

 (+info)

Evaluation of a laboratory-scale bioreactive in situ sediment cap for the treatment of organic contaminants. (5/89)

 (+info)

Optimisation of surface expression using the AIDA autotransporter. (6/89)

 (+info)

Parametric optimization of feruloyl esterase production from Aspergillus terreus strain GA2 isolated from tropical agro-ecosystems cultivating sweet sorghum. (7/89)

A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71- 0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of 30 degrees C. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions.  (+info)

Repeated-batch operation of immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor for lactose hydrolysis. (8/89)

In this study, we investigated the performance of an immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor, where the cells were immobilized in alginate beads, which were then used in repeated-batch operations for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose over the long-term. In particular, in the Tris buffer system, disintegration of the alginate beads was not observed during the operation, which was observed for the phosphate buffer system. The o-nitrophenyl-beta-D-galactoside hydrolysis was operated successfully up to about 80 h, and the runs were successfully repeated at least eight times. In addition, hydrolysis of lactose was successfully carried out up to 240 h. Using Western blotting analyses, it was verified that the beta-galactosidase inclusion bodies were sustained in the alginate beads during the repeated-batch operations. Consequently, we experimentally verified that beta-galactosidase inclusion bodies-containing Escherichia coli cells could be used in a repeated-batch reactor as a biocatalyst for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose. It is probable that this approach can be applied to enzymatic synthesis reactions for other biotechnology applications, particularly reactions that require long-term and stable operation.  (+info)

Batch cell culture techniques refer to a method of growing cells in which all the necessary nutrients are added to the culture medium at the beginning of the growth period. The cells are allowed to grow and multiply until they exhaust the available nutrients, after which the culture is discarded. This technique is relatively simple and inexpensive but lacks the ability to continuously produce cells over an extended period.

In batch cell culture, cells are grown in a closed system with a fixed volume of medium, and no additional nutrients or fresh medium are added during the growth phase. The cells consume the available nutrients as they grow, leading to a decrease in pH, accumulation of waste products, and depletion of essential factors required for cell growth. As a result, the cells eventually stop growing and enter a stationary phase, after which they begin to die due to lack of nutrients and buildup of toxic metabolites.

Batch cell culture techniques are commonly used in research settings where large quantities of cells are needed for experiments or analysis. However, this method is not suitable for the production of therapeutic proteins or other biologics that require continuous cell growth and protein production over an extended period. For these applications, more complex culture methods such as fed-batch or perfusion culture techniques are used.

Cell culture is a technique used in scientific research to grow and maintain cells from plants, animals, or humans in a controlled environment outside of their original organism. This environment typically consists of a sterile container called a cell culture flask or plate, and a nutrient-rich liquid medium that provides the necessary components for the cells' growth and survival, such as amino acids, vitamins, minerals, and hormones.

There are several different types of cell culture techniques used in research, including:

1. Adherent cell culture: In this technique, cells are grown on a flat surface, such as the bottom of a tissue culture dish or flask. The cells attach to the surface and spread out, forming a monolayer that can be observed and manipulated under a microscope.
2. Suspension cell culture: In suspension culture, cells are grown in liquid medium without any attachment to a solid surface. These cells remain suspended in the medium and can be agitated or mixed to ensure even distribution of nutrients.
3. Organoid culture: Organoids are three-dimensional structures that resemble miniature organs and are grown from stem cells or other progenitor cells. They can be used to study organ development, disease processes, and drug responses.
4. Co-culture: In co-culture, two or more different types of cells are grown together in the same culture dish or flask. This technique is used to study cell-cell interactions and communication.
5. Conditioned medium culture: In this technique, cells are grown in a medium that has been conditioned by previous cultures of other cells. The conditioned medium contains factors secreted by the previous cells that can influence the growth and behavior of the new cells.

Cell culture techniques are widely used in biomedical research to study cellular processes, develop drugs, test toxicity, and investigate disease mechanisms. However, it is important to note that cell cultures may not always accurately represent the behavior of cells in a living organism, and results from cell culture experiments should be validated using other methods.

Culture techniques are methods used in microbiology to grow and multiply microorganisms, such as bacteria, fungi, or viruses, in a controlled laboratory environment. These techniques allow for the isolation, identification, and study of specific microorganisms, which is essential for diagnostic purposes, research, and development of medical treatments.

The most common culture technique involves inoculating a sterile growth medium with a sample suspected to contain microorganisms. The growth medium can be solid or liquid and contains nutrients that support the growth of the microorganisms. Common solid growth media include agar plates, while liquid growth media are used for broth cultures.

Once inoculated, the growth medium is incubated at a temperature that favors the growth of the microorganisms being studied. During incubation, the microorganisms multiply and form visible colonies on the solid growth medium or turbid growth in the liquid growth medium. The size, shape, color, and other characteristics of the colonies can provide important clues about the identity of the microorganism.

Other culture techniques include selective and differential media, which are designed to inhibit the growth of certain types of microorganisms while promoting the growth of others, allowing for the isolation and identification of specific pathogens. Enrichment cultures involve adding specific nutrients or factors to a sample to promote the growth of a particular type of microorganism.

Overall, culture techniques are essential tools in microbiology and play a critical role in medical diagnostics, research, and public health.

Bacteriological techniques refer to the various methods and procedures used in the laboratory for the cultivation, identification, and study of bacteria. These techniques are essential in fields such as medicine, biotechnology, and research. Here are some common bacteriological techniques:

1. **Sterilization**: This is a process that eliminates or kills all forms of life, including bacteria, viruses, fungi, and spores. Common sterilization methods include autoclaving (using steam under pressure), dry heat (in an oven), chemical sterilants, and radiation.

2. **Aseptic Technique**: This refers to practices used to prevent contamination of sterile materials or environments with microorganisms. It includes the use of sterile equipment, gloves, and lab coats, as well as techniques such as flaming, alcohol swabbing, and using aseptic transfer devices.

3. **Media Preparation**: This involves the preparation of nutrient-rich substances that support bacterial growth. There are various types of media, including solid (agar), liquid (broth), and semi-solid (e.g., stab agar). The choice of medium depends on the type of bacteria being cultured and the purpose of the investigation.

4. **Inoculation**: This is the process of introducing a bacterial culture into a medium. It can be done using a loop, swab, or needle. The inoculum should be taken from a pure culture to avoid contamination.

5. **Incubation**: After inoculation, the bacteria are allowed to grow under controlled conditions of temperature, humidity, and atmospheric composition. This process is called incubation.

6. **Staining and Microscopy**: Bacteria are too small to be seen with the naked eye. Therefore, they need to be stained and observed under a microscope. Gram staining is a common method used to differentiate between two major groups of bacteria based on their cell wall composition.

7. **Biochemical Tests**: These are tests used to identify specific bacterial species based on their biochemical characteristics, such as their ability to ferment certain sugars, produce particular enzymes, or resist certain antibiotics.

8. **Molecular Techniques**: Advanced techniques like PCR and DNA sequencing can provide more precise identification of bacteria. They can also be used for genetic analysis and epidemiological studies.

Remember, handling microorganisms requires careful attention to biosafety procedures to prevent accidental infection or environmental contamination.

Culture media is a substance that is used to support the growth of microorganisms or cells in an artificial environment, such as a petri dish or test tube. It typically contains nutrients and other factors that are necessary for the growth and survival of the organisms being cultured. There are many different types of culture media, each with its own specific formulation and intended use. Some common examples include blood agar, which is used to culture bacteria; Sabouraud dextrose agar, which is used to culture fungi; and Eagle's minimum essential medium, which is used to culture animal cells.

... β-lactam The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly ... the potency of each batch of penicillin varied from batch to batch. It was therefore impossible to prescribe 1 g of penicillin ... This causes the cell wall to weaken due to fewer cross-links and means water uncontrollably flows into the cell because it ... This is because Gram-positive bacteria do not have an outer cell membrane and are simply enclosed in a thick cell wall. ...
Their batch of cultures lasted from 1912 to 1946. Because the tissue cultures far outlived their organisms, they were deemed " ... At Yale, Burrows also successfully established tissue cultures of embryonic chick cells. Upon returning to Carrel's laboratory ... One significant innovation was the method they developed for serial activation of tissues in culture. This technique ... Culturing Life: How Cells Became Technologies. Harvard University Press, Cambridge. ISBN 9780674039902. Burrows, Montrose T., ...
2. High cell density (High cell concentration)[1] In a batch culture, to achieve very high cell concentrations, e.g. 50-100 g ... Fed-batch culture is a powerful technique to do so. Examples of the repressible promoter are trp promoter and phoA promoter. 7 ... fed-batch culture is superior to conventional batch culture when controlling concentrations of a nutrient (or nutrients) ... This type of the fed-batch culture is named constantly-fed-batch culture (CFBC), and is well established mathematically and ...
They were the first calves to be produced using standard cell-culturing techniques. In 2001, Brazil cloned their first heifer, ... the first batch of monotocous cloned police dogs was born. In 1958, John Gurdon, then at Oxford University, explained that he ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell. 172 (4): 881-887.e7. doi:10.1016/j.cell. ... In 1986, the first mouse was cloned in the Soviet Union from an embryo cell. The first mouse from adult cells, Cumulina, was ...
In 2006, Schärfe System was acquired by Innovatis AG, a company focused on cell culture analysis. CASY utilizes the techniques ... It would not be necessary to detect the cells from a batch to another batch but measure them continuously and smoothly. CASY ... As the cell size is related to the cell volume, a cell size profile in cell population can be produced in terms of pulse height ... This technology can be applied for cell counting, cell culture analysis at a certain time interval, or even a period of time. ...
One of the most common techniques is utilizing fed-batch culture. Fed-batch culture is, in the broadest sense, defined as an ... In microbial cells, the cell walls are either Gram-positive or Gram-negative. These outcomes are based on the Gram Stain test. ... A Gram-negative cell wall is more resistant to such attacks and more difficult to destroy. The membrane of microbial cells are ... Microbial cell factory is an approach to bioengineering which considers microbial cells as a production facility in which the ...
... microfabrication techniques are also used to create 3D microstructures which allow the patterning of cell culture surfaces ... However, Matrigel has significant disadvantages such as a xenogeneic origin, bath-to-batch variability, high cost and a poorly ... Apart from co-culturing intestinal cells with other cell types, also the cell population of the intestinal epithelium is of ... such as traditional 2D cell culture of immortalised cell lines (e.g. CaCo2 or HT29), transwell cultures, Ussing chambers, and ...
After the cells reach steady-state physiology (i.e., constant metabolite concentrations in culture), cells are then lysed to ... the analysis is limited to batch cultures during the exponential phase. Moreover, after addition of a labeled substrate, the ... This technique for flux quantitation is applicable under metabolic and isotopic steady-state, two conditions that assume that ... The first step in the workflow of isotope labeling experiments is cell culture on labeled substrates. A substrate such as ...
Generally performs with a constant error rate as an off-line (batch) type process. A range of image classification techniques ... To quantify the number of cells in a culture, the cells can be simply plated on a petri dish with growth medium. If the cells ... require cell counting. Calculating the fraction of dead to live cells as a measure of cell viability, such as of cells exposed ... Cell suspensions are turbid. Cells absorb and scatter the light. The higher the cell concentration, the higher the turbidity. ...
... secreted from the bacterial cells and ultimately recovered from the media and purified using alcohol precipitation techniques. ... In this process, a preserved culture of the gram-negative bacterium is expanded through growth and then used as an inoculum in ... Under select modes of operation, such as batch fermentation, and proper growth conditions, fermentation then takes place. ... gene cluster also plays an important role in the plant-bacteria interactions by encoding for a cell-cell signaling system ...
Fed-batch systems mean inoculating the cells, culturing them and harvesting them in a single period. Stirred tank bioreactors ... Cultured meat is produced using tissue engineering techniques pioneered in regenerative medicine. Jason Matheny popularized the ... Cellular agriculture requires cell lines, generally stem cells. Stem cells are undifferentiated cells which have the potential ... the density of the cells on the scaffold means that not all cells have an interface with culture media, leading to cell death ...
There would need to be a way to determine how many cells survived at the end of the incubation period using batch cultures. ... The improved pipetting technique was described in 2011 in the study of the biosafety level 3 (BSL-3) pathogen Bacillus ... such as a delay due to the time required for the repair of damaged cell structures such as cell walls or cell membranes, are ... At this time, cells exposed to no antimicrobial agent were added to several wells of the plate from the culture kept on ice; ...
Cancer Cell Lines and Primary Cells Retrieved 2011-10-14. (Molecular biology techniques, Gene expression). ... Finally, cultured cells are poured into the dish for plasmid uptake. However, with the invention of different types of ... Effectene Reagent spontaneously forms micelle structures exhibiting no size or batch variation (as may be found with pre- ... Reverse transfection is a technique for the transfer of genetic material into cells. As DNA is printed on a glass slide for the ...
For example, Vero cell or other tissue cultures may be used to investigate an influenza virus or coronavirus, while various ... Special computer systems have been designed with the ability to scan samples in batches. The appearance of the plaque depends ... or using techniques such as staining (e.g. neutral red for eukaryotes or giemsa for bacteria) or immunofluorescence. ... A viral plaque is a visible structure formed after introducing a viral sample to a cell culture grown on some nutrient medium. ...
In horticultural settings batches can be orders of magnitude greater. Silage technology may be usable if it is adapted to ... An industrial-scale technique mimics the windrows of large-scale composting, except that bokashi windrows are compacted, ... Biogas production does not burn the carbon, but the bacterial culture is optimized to extract the carbon in the form of methane ... It can pass through cell membranes and almost all living organisms have the enzyme lactate dehydrogenase to convert it to ...
Phelan K, May KM (March 2015). "Basic techniques in mammalian cell tissue culture". Current Protocols in Cell Biology. 66 (1): ... because the research laboratory methods of creating small batches had not yet been translated to commercial large-batch ... ISBN 978-0-471-19350-0. Schantz JT, Ng KW (2004). A manual for primary human cell culture. World Scientific. p. 89. Comroe JH ( ... is used in a standard antibiotic cocktail to prevent bacterial infection in cell culture. When purifying protein from a ...
... could be used to identify cell cultures infected with virus and also cultures with antibodies to virus. This pH could be easily ... 1. He proved a method for separation of monkey kidney cells, which led to techniques for large scale production. 2. He ... The measurement developed by Youngner for safely and quickly testing batches of vaccine and also antibodies to the virus after ... Youngner demonstrated the separation of monkey kidney cells using the pancreatic enzyme trypsin, a technique previously proven ...
Culture-media conditioned by macrophages can be used. Production in cell culture is usually preferred as the ascites technique ... The antibody-containing medium is then incubated with the immobilized antigen, either in batch or as the antibody is passed ... single B cell culture, single cell amplification from various B cell populations and single plasma cell interrogation ... similar to a cancer cell). This mixture of cells is then diluted and clones are grown from single parent cells on microtitre ...
Researchers have also aimed to improve cultivation techniques using a high-biomass batch culturing technique. One of the most ... This bacteria is a gram-negative kidney-bean-shaped cell that deposits iron oxides on the concave side of the cell, forming ... These cultivation techniques follow those found in Emerson and Floyd (2005). Recently, researchers have been able to culture ... Barco, R. A.; Edwards, K. J. (2014). "Interactions of proteins with biogenic iron oxyhydroxides and a new culturing technique ...
As they are manufactured using recombinant bacterial production processes, the batch-to-batch consistency for Affimers is ... Multimeric forms Affimers have been generated and shown to yield titres in the range of 200-400 mg/L under small-scale culture ... Affimers as therapeutics are in discovery and preclinical development to tackle cancer, both via CAR-T cell therapy and as ... The ability to direct in vitro screening techniques allows the identification of specific, high affinity Affimers. In vitro ...
... the fermenting mass of fruit-called the motor or kicker in prison parlance-is retained from batch to batch to make the ... Food hoarding in the inmate cells in both prisons and jails allows the inmates to acquire ingredients and produce pruno. During ... Though popularized in prison fiction, these techniques are slow and laborious, and generally result in a low alcohol content. ... Bum wine Changaa Chicha Drinking culture Jenkem Kilju Kvas Moonshine (hooch) Pájaro verde Poitín Bootlegging Tepache Tharra ...
... has been discovered using culture-independent techniques such as metagenomic sequence analysis. Some notable species within ... which holds the ribosomes and DNA of the cell. The anammoxosome is a membrane-bound component of the cell that does not contain ... Brocadia and others are used in industrial waste water treatment plants using sequencing batch reactors, rotating biological ... ed.). Planctomycetes: Cell Structure, Origins and Biology, Humana Press. Totowa, NJ: Springer. pp. 1-38. Jetten MSM, Op Den ...
Ongoing research is performed to develop in situ separation techniques, where product is removed from the batch during the ... Whole cells are typically used when a reaction requires a co-factor. Although co-factors may be generated in vitro, it is ... Extracellular enzymes are released into the culture medium, and are much simpler to separate. Enzymes must maintain their ... These immobilization techniques occur at ambient temperatures in mild conditions, which have limited potential to modify the ...
... to restart due to a chemical compound released by dying yeast cells that inhibit the future growth of yeast cells in the batch ... There are various techniques that a winemaker can employ to minimize the chances of a stuck fermentation happening. The most ... such as adding nitrogen to the must in the form of diammonium phosphate or using cultured yeast with a high temperature and ... to a chemical compound released by the dying yeast that serves as an inhibitor to the growth of future yeast cells in the batch ...
Cultured meat (also known by other names) is a meat produced by in vitro cell cultures of animal cells. It is a form of ... with large batch-to-batch variation. Cultured meat companies have been putting significant resources into alternative growth ... Cultured meat is produced using tissue engineering techniques traditionally used in regenerative medicines. The concept of ... making milk from cell culture, now Perfect Day Foods), The EVERY Company (making egg whites from cell culture), Gelzen (making ...
A newer cell separation technique employing antibodies is buoyancy-activated cell sorting (BACS) is a separation technique in ... neural progenitor cell) cultures in particular, as it is easier to manage and causes minimal damage to live cells. NPC cultures ... especially when the target cells are comparatively rare and a large batch of cells must be processed. Moreover, flow cytometry ... Individual cells are deflected by either a force directly on the cell or a force on the fluid surrounding the cells, so that ...
Ryōta Momoi's 4koma manga will be released in print book format under the title KanColle 4 cell comic: Fubuki, I will do my ... "Kantai Collection". Culture Japan. Danny Choo. 2013-10-10. Archived from the original on 2015-03-24. Retrieved 2015-02-12. ... of the initial retail batch stock was sold out within the first week. Kadokawa Games has announced that the game will be no ... where senior girls teach battle techniques to their juniors. It has been updated weekly since October 2013. ...
HCMV replicates within infected endothelial cells at a slow rate, taking about five days in cell culture. It also infects ... An ELISA technique for CMV-specific IgM is available, but may give false-positive results unless steps are taken to remove ... The major disadvantage of the pp65 assay is that only a limited number of samples can be processed per test batch. CMV should ... Phenotypic resistance testing involves cultivation of the virus in cell culture and testing its susceptibility using different ...
Immunohistochemistry and immunocytochemistry are methods used to determine in which cells or parts of cells a particular ... Related techniques are also useful in sub-typing lymphocytes which all look quite similar on light microscopy. In laboratory ... Now, many are monoclonal, i.e. produced in tissue culture. Monoclonal antibodies that consist of only one type of antibody tend ... to provide greater antigen specificity, and also tend to be more consistent between batches. The first step in immunoperoxidase ...
... causing the cell to be unable to separate its chromosomes during the metaphase. The cell then undergoes apoptosis. The ... Its use for being anti-tumor, anti-mutagenic are well documented in ancient system of medicine of Ayurveda and folk culture of ... It can also now be synthesized through a stereocontrolled total synthesis technique which retains the correct stereochemistry ... A significant series of inadvertent intrathecal vincristine administration occurred in China in 2007 when batches of cytarabine ...
Rochester, N.; J.H. Holland, L.H. Habit and W.L. Duda (1956). "Tests on a cell assembly theory of the action of the brain, ... Analytical and computational techniques derived from statistical physics of disordered systems, can be extended to large-scale ... or by grouping examples in so-called mini-batches. A. K. Dewdney, a former Scientific American columnist, wrote in 1997, " ... computing Cerebellar model articulation controller Cognitive architecture Cognitive science Connectionism Connectomics Cultured ...
"Batch Cell Culture Techniques" by people in this website by year, and whether "Batch Cell Culture Techniques" was a major or ... Batch Cell Culture Techniques*Batch Cell Culture Techniques. *Batch Culture Techniques. *Batch Culture Technique ... "Batch Cell Culture Techniques" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Below are the most recent publications written about "Batch Cell Culture Techniques" by people in Profiles. ...
Culture Techniques [E05.481.500] * Cell Culture Techniques [E05.481.500.249] * Batch Cell Culture Techniques [E05.481.500.249. ... Batch Culture Batch Culture Techniques Continuous Batch Culture Fed Batch Culture Techniques Fed-Batch Culture Fed-Batch ... Fed-Batch Culture Techniques Narrower Concept UI. M0556825. Scope Note. Batch cell culture technique where the culture is fed ... Batch Cell Culture Techniques Preferred Concept UI. M0556824. Scope Note. Methods for cultivation of cells, usually on a large- ...
According to ESACT, the award recognizes outstanding innovators in the field of Animal Cell Culture Technology (ACCT) and ... Keywords: Awards and Prizes; Bioreactors; Batch Cell Culture Techniques; Vaccines; Cell- and Tissue-Based Therapy; Genetic ... According to ESACT, the award recognizes outstanding innovators in the field of Animal Cell Culture Technology (ACCT) and ... Scientists recognized for their outstanding contributions in automated cell culture technologies to support clonal selection ...
For example, 2D cell culturing techniques hinder continuous batch processing opportunities as they are limited by cell ... EV production in 3D cell culture can help circumvent this issue and enable continuous batch processing. At present, there is ... For instance, Corning® HYPERStack® 12-layer cell culture vessels have demonstrated a capacity to produce up to seven trillion ... Corning CellBIND® HYPERStack 12 Layer Cell Culture Vessel. Just as there are two major pathways for investigating their ...
Experience culturing and passaging of adherent or suspension cells with aseptic techniques ... Write and review technical documentation from writing technical SOPs, draft manufacturing batch records, technical reports, and ... Our research programs are rooted in leveraging T cell biology to generate NKT and T cell subtypes from hematopoietic stem cells ... Research Associate or Research Associate II to be part of the Process Design team with previous cell culture experience. The ...
... β-lactam The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly ... the potency of each batch of penicillin varied from batch to batch. It was therefore impossible to prescribe 1 g of penicillin ... This causes the cell wall to weaken due to fewer cross-links and means water uncontrollably flows into the cell because it ... This is because Gram-positive bacteria do not have an outer cell membrane and are simply enclosed in a thick cell wall. ...
Learn how to optimize your stem cell culture in bioreactors ... A number of techniques exist including batch, fed-batch, ... Stem cell bioprocessing requires stringent conditions and strict protocols. ... Cell-only aggregates: This technique involves allowing cells to adhere to one another and form an aggregate. This technique is ... Stem cell expertise at Eppendorf Eppendorf has a long, established history working with stem cell processes in 3D cultures. ...
Batch Cell Culture Techniques 16% * Stability and activity of lipases on exposure to oxidative environments. Törnvall, U., ... Competitive capacitive biosensing technique (CCBT): A novel technique for monitoring low molecular mass analytes using glucose ... Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli. Idil, N., Hedström, M., ... Capacitive immunosensor for the detection of host cell proteins.. Teeparuksapun, K., Hedström, M., Kanatharana, P., ...
Cell and Tissue Culture Techniques. This course provides students with a sound, practical, and theoretical knowledge of key… ... Participants will learn the importance and benefits of having and completing Batch Production… more ... Cell and Gene Therapy is a technique that modifies a persons genes to treat or cure disease.… more ... This course is for operators, supervisors, and managers responsible for batch process operations… more ...
Batch Cell Culture Techniques 100% * Epidermal Growth Factor 19% * Glycerol 75% * Growth 20% ... Dive into the research topics of Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant ... Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1. ...
This article on cell culture basics describes different techniques such as adherent vs suspension and 2D vs 3D methods. ... Cell banking Cell banking refers to the process of storing multiple batches of a specific cell type for later use, to avoid ... What is cell culture? Cell culture Cell culture refers to the process of growing cells under controlled conditions outside ... CH/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cell-culture- ...
The standard procedure for growing E. coli cells to high cell densities is the fed-batch technique, where the carbon substrate ... For example, in humans variability in cell density among cells of a given cell type is 100 times smaller than variation in cell ... What cell density tells us?. Cell density shows very little variation within a given cell type. ... This tight control indicates that maintenance of a cell type-specific cell density is important for cell function. ...
Batch Cell Culture Techniques 10% * Bacteria 10% * Alginates 8% * Immobilization 7% Earth & Environmental Sciences. * phthalate ...
Batch Cell Culture Techniques 64% * Mycotoxins 60% * feces 52% 31 Citations (Scopus) ... Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells. Gratz, S., Wu, Q. ... rapidly hydrolyze the masked mycotoxin deoxynivalenol-3-glucoside and release deoxynivalenol in spiked batch cultures in vitro ... Gratz, S. & El-Nezami, H. S., 2011, Protective Cultures, Antimicrobial Metabolites and Bacteriophages for Food and Beverage ...
Batch Cell Culture Techniques 84% * Mass Average Molar Mass 70% * Molecular weight 67% ... of high and ultra-high molecular weight by Azotobacter vinelandii in batch and fed-batch cultures. Castillo, T., Flores, C., ... Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA. Vergara, M., Becerra, S., ... Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures. ...
... the startup has developed a continuous cell culture system that enables high yields at lower costs. ... CellulaREvolutions technology is different from existing traditional batch-based cell culturing techniques, which are not able ... Unlike traditional batch technologies in the sector, the startup has developed a continuous cell culture system that enables ... CellulaREvolution Bags US$1.37 Million To Scale Continuous Cell Culturing Tech System. Cell-Based NewsAlt ProteinFuture Foods ...
Batch Cell Culture Techniques Medicine & Life Sciences 7% * Penicillin-Binding Proteins Medicine & Life Sciences 6% ... In addition, batch culture growth kinetics assays will be crucial to learning the cellular and molecular mechanisms responsible ... In addition, batch culture growth kinetics assays will be crucial to learning the cellular and molecular mechanisms responsible ... In addition, batch culture growth kinetics assays will be crucial to learning the cellular and molecular mechanisms responsible ...
Batch Cell Culture Techniques Medicine & Life Sciences 26% * Efficiency Medicine & Life Sciences 20% ... Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. However ... Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. However ... Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. However ...
Cell Culture. Batches of mosquitoes were identified to species or genus level, separated into pools of 30 individuals (maximum ... serratus mosquitoes sampled in Coronel Barros (Table 2). All YFV-positive pools showed positive results with the 3 techniques ... and added to cultures of Vero cells. The cells were observed for 14 days; after this period, cultures were harvested and ... which was only positive by cell culture and by reverse transcription-PCR. ...
View MoreAnalytical MethodsBioBusinessCell Culture/FermentationContinuous ProcessingDrug DeliveryFill/FinishFormulationProcess ... detection of cell viability without conventional staining techniques; and on-line monitoring of enzyme carriers to determine ... Applications include in-line monitoring of Hansenula anomala culture in a batch fermentation process; real-time monitoring of ... Cell metabolism and cell growth also can be measured (30). Teixeira and colleagues monitored on-line cell viability of ...
Depending on the species of the serum, each batch of serum is tested for adventitious viruses using cell culture techniques. ... The sera are tested for the absence of Mycoplasma using a cell culture assay in Axcell Biotechnologies media by culture method ... Each batch of serum is manufactured for maximum in vitro growth support of particular cell lines. ... The biological performance is assessed using cell culture medium supplemented with a final concentration of 10% serum. During ...
... we will also optimize cell culturing techniques for the bioreactor. We will determine the role of nutrients in the cell culture ... and we will begin manufacturing larger batches of the membranes for testing in the full-scale cartridges. ... 7. What about stem cells?. Cells grown in culture simply do not function the same as cells taken from human kidneys, a feature ... We culture regenerated cells from these failed transplants. The bioreactor then continuously suffuses the cultured cells with ...
AnatomyBioprocessingBioreactorBiotechnology equipmentsCell cultureCellsClinical laboratory techniqueCytological technique ... Moreover the concept of "batch" is turned on its head. "With proposed applications involving one batch per patient in many ... Stem Cells and Biosimilars. Scaling up stem cell cultures presents a maze of challenges quite different from those facing the ... This automated technique provides extremely high-quality images of cells. By reading the contents of 96 wells in less than four ...
eds), Cell Culture Techniques, Neuromethods, vol. 56 (2011) Springer Science+Business Media. ... Seasonal and regional variations in serum composition, resulting in batch-to-batch variations, further aggravate the situation ... Reference cell culture. Aggregating brain cell cultures were used as a model for LP testing. This 3D primary cell culture ... serum-free/chemically defined cell culture media are still rarely used for mammalian cells. Most standard cell culture ...
In the year 2023, the industry size of cell culture consumables was USD 12 billion. North America is estimated to hold the ... The cell culture consumables market size is set to cross USD 400 billion by the end of 2036, expanding around 23% CAGR during ... largest revenue share of 32% by the end of the forecast period, backed by increase in research into stem cells and cancer along ... increasing the demand for cell culture consumables as they are used in cell culture techniques. ...
Cell division during such a nutritional shift-down was studied in batch cultures and with the baby-machine technique. ... Following a brief delay, the rate of division was maintained for 60 to 70 min in batch cultures and for an extended period in ... Decreases in cell size were due, in part, to a possible reduction in the mass per chromosome origin at the time of replication ... growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell. ...
At IQ Products we make use of a range of cell culture techniques to provide you with the most cost effective option for your ... We offer you conjugation of a volume as little as 1 mg of antibody up to large-scale single batches and the purification of the ... We can offer static flask, spinner flask and hollow fiber bioreactor cultures to meet your needs from 10 mg to 10 g. ... Thanks to our ISO 13485:2016 certified quality system, IQ Products offers you lot-to-lot consistency and batch-tested ...
... using cell culture, tissue engineering, and, more recently, bioprinting approaches. This study assessed the manufacturing ... Methods Two batches (40 cm2 each) of Poieskin® were produced and their reliability and homogeneity were assessed using a ... using cell culture, tissue engineering and more recently bioprinting approaches. This study assessed the manufacturing ... Two batches (40 cm2 each) of Poieskin® were produced, and their reliability and homogeneity were assessed using histological ...
Biotherapeutics are produced using genetically modified, live mammalian and microbial cells and require cell culture media for ... Historically, these therapeutics have been made with batch-processing techniques. Think of it like brewing beer - but instead ... The device can be used to rapidly monitor culture media for nutrients and supplements to improve product yield and quality. ... "Were trying introduce analytical techniques that are faster and can be done inline or at-line during manufacturing - ...
  • Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)
  • The bioreactor then continuously suffuses the cultured cells with concentrations of nutrients and hormones that mimic the body's natural state. (vumc.org)
  • We can offer static flask, spinner flask and hollow fiber bioreactor cultures to meet your needs from 10 mg to 10 g. (iqproducts.nl)
  • When culture volume and cell density are optimal, the culture is then transferred to a production bioreactor. (sigmaaldrich.com)
  • Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)
  • Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS). (sigmaaldrich.com)
  • Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • Figure 1: Comparison of analyzers with respect to their ease of implementation in microbial fermentations and mammalian cell culture unit operations for various quality attributes: (a) misincorporation, (b) nutrients, (c) glycosylation, and (d) cell growth. (biopharminternational.com)
  • Mammalian cell culture reactors were adapted from chemical mixing tanks, which used shaft-and-impeller mixing. (genengnews.com)
  • Knowledge of basic immunology and cell biology techniques, including mammalian cell culture, is required. (affyimmune.com)
  • Unlike whole cell therapies, EVs are acellular products, the equivalent of text messages sent back and forth in order to elicit reactions. (corning.com)
  • Their small size confers other benefits, as well - too small to convey pathogens such as bacteria or viruses, EVs are also incapable of the unwanted replication or tumor formation that whole cell therapies can occasionally provoke. (corning.com)
  • The potential EVs possess for the field of acellular therapies - treatments derived from acellular products that achieve the same therapeutic response without delivering entire cells - is an exciting one. (corning.com)
  • Appia Bio is a preclinical stage biotech company founded in 2020 with the mission to bring innovative off-the-shelf cell therapies to cancer patients. (berkeley.edu)
  • Our goal is to discover and develop allogeneic cell therapies across a broad array of cancer indications with a scalability that increases access for patients. (berkeley.edu)
  • The consortium specifically focuses on the manufacturing of protein therapies like antibodies, gene therapies, cell therapies, and vaccines. (unc.edu)
  • Quell Therapeutics Ltd ("Quell"), the world leader in the development of engineered T-regulatory (Treg) cell therapies for serious medical conditions driven by the immune system, announced it has raised $156 million in an oversubscribed Series B financing. (bios.community)
  • In recent years, cell therapies and other Advanced Therapy Medicinal Products (ATMPs) have begun to come into their own as highly promising treatments for a wide range of serious, chronic and rare diseases. (amerisourcebergen.com)
  • With a record number of new cell and gene therapies (CGTs) approved in 2021( 1 ) , a surge in commercializations is anticipated. (amerisourcebergen.com)
  • During a recent roundtable hosted by The Medicine Maker, Andrea Zobel, Senior Director, Personalized Supply Chain at World Courier, explored the complexities of bringing cell therapies to the market. (amerisourcebergen.com)
  • There are a few transport obstacles that need to be overcome to ensure successful commercialization of cell therapies. (amerisourcebergen.com)
  • Batch cell culture technique where the culture is fed with nutrients throughout the process, instead of all at once in the beginning. (nih.gov)
  • The cells in the kidney's tubule grow easily in culture, and use chemical energy derived from the body's nutrients to do the concentrating, so our implantable artificial kidney does not need to rely on electrical power and pumps. (vumc.org)
  • This phenomenon is known as "cell culture stress," and it happens because cells cannot be continuously supplied with fresh nutrients and oxygen like they are in the body. (vumc.org)
  • In the organism, liposoluble nutrients such as cholesterol, fatty acids and lipophilic vitamins reach the cells by means of specific apolipoproteins which form complexes with the lipids, the so-called lipoproteins (LPs), and which recognize specific membrane receptors expressed by the cells. (forschung3r.ch)
  • The device can be used to rapidly monitor culture media for nutrients and supplements to improve product yield and quality. (unc.edu)
  • Because cell lines are unique and require different nutrients to thrive, culture biomass and protein production are largely dependent on the culture media used. (sigmaaldrich.com)
  • PBS' U-shaped bioreactors employ a patented mixing technology, the Air-Wheel™, that exploits the fact that cell cultures must be oxygenated. (genengnews.com)
  • To support the development of monoclonal antibodies, manufacturers have constructed sizable manufacturing facilities with several cell culture bioreactors. (researchnester.com)
  • Bioreactors are large scale culture devices used in upstream processes and production that play a key role in expanding and scaling cell culture for production. (sigmaaldrich.com)
  • The typical seed train cell expansion process involves thawing cryopreserved cells followed by successive expansions into larger culture vessels including flasks, spinners, and stirred bioreactors. (sigmaaldrich.com)
  • Bioreactors provide controlled microenvironments and nutrient delivery to regulate cell growth and differentiation, improving standardization and reproducibility. (sigmaaldrich.com)
  • PBS offers scalability from three liters for lab work, and 80 liters for preclinical and clinical batches. (genengnews.com)
  • AffyImmune Therapeutics ("Affy"), a clinical stage biotechnology company focused on developing technologies that expand the utility and improve the safety and efficacy of cell-based immunotherapy for cancer, is seeking an Analytical Development Scientist to help advance the use of CAR T therapy for solid tumors. (affyimmune.com)
  • Assist in development of clinical manufacturing documents (IND amendments, master batch records, etc. (affyimmune.com)
  • However, to facilitate their clinical use and to ensure safety, iPS culturing protocols must be compliant with good manufacturing practice guidelines and devoid of xenogenic products. (biomedcentral.com)
  • This is a multi-disciplinary approach that integrates molecular, biochemical and clinical techniques with developmental and engineering processes. (openorthopaedicsjournal.com)
  • Our research programs are rooted in leveraging T cell biology to generate NKT and T cell subtypes from hematopoietic stem cells. (berkeley.edu)
  • The production of monoclonal antibodies is being carried out on a wide scale employing group, ongoing, and perfusion culture techniques. (researchnester.com)
  • Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest. (rush.edu)
  • Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
  • These growth strategies, together with optimization of media composition and the application of molecular biology methods, made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation. (pfeiffertheface.com)
  • Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
  • In the present work, a combination of two methods was tested: FACS combined with growing cells in 96-well plates containing solid agar growth medium to accelerate both the isolation procedure and culture scale-up. (biomedcentral.com)
  • METHODS: HEK293 (human epithelial kidney) and swine testis (ST) cells were co-cultured with macroencapsulated pig islets embedded in an alginate patch, macroencapsulated PK15 (swine kidney epithelial) cells embedded in an alginate patch and free PK15 cells. (bvsalud.org)
  • Extracellular vesicles act as a cell's transfer signals - these nanoparticles, which contain protein, sugars, and other biological materials, communicate with adjacent cells and tissues to transmit messages, such as whether a cell is healthy or in distress. (corning.com)
  • The implantable bioartificial kidney combines nano-technology with tissues from human kidney cells. (vumc.org)
  • 1) One is to replace serum by other biological but as yet inadequately defined mixtures such as extracts from plants, animal tissues or cells. (forschung3r.ch)
  • Tissues and Cells. (lu.se)
  • In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
  • 4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)
  • In addition, batch culture growth kinetics assays will be crucial to learning the cellular and molecular mechanisms responsible for susceptibility and resistance to EIPE-1. (okstate.edu)
  • Prior experience with the design, execution, optimization, and validation of a broad range of in vitro cell based assays is required. (affyimmune.com)
  • Experienced and well-versed with FDA Guidance related to CAR T cell therapy manufacturing and release testing and industry whitepapers for state-of-the-art method validation of manufacturing assays. (affyimmune.com)
  • Detailed understanding of immunology and cell therapy. (bios.community)
  • and we will begin manufacturing larger batches of the membranes for testing in the full-scale cartridges. (vumc.org)
  • It is an important building block of all cell membranes, and an important metabolite in many biosynthetic pathways. (forschung3r.ch)
  • The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
  • The standard procedure for growing E. coli cells to high cell densities is the fed-batch technique, where the carbon substrate, e.g. glucose, controls the growth as a limiting factor. (pfeiffertheface.com)
  • This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
  • Several mammalian cell lines, including CHO, lymphoma (NS0, SP2/0) and human embryonic kidney (HEK) cells, have been developed and used to produce therapeutic proteins and antibodies at high levels. (sigmaaldrich.com)
  • Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
  • Emerging market trends include improvements in the creation and formulation of cell culture medium. (researchnester.com)
  • Demonstrable expertise in cell-based techniques including cell enrichment, culture, and formulation technologies. (bios.community)
  • An important aspect is to look at the raw materials used in the formulation of the cells and how they are packaged up in that formulation. (amerisourcebergen.com)
  • Cell Culture Consumables Market size is set to cross USD 400 billion by the end of 2036, expanding around 23% CAGR during the forecast period i.e., 2024 - 2036. (researchnester.com)
  • The FDA has issued a draft guideline on new cell substrates for vaccine manufacturing to detail requirements in this area. (gmp-compliance.org)
  • Biotherapeutics are produced using genetically modified, live mammalian and microbial cells and require cell culture media for their development. (unc.edu)
  • Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
  • In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
  • This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
  • We are now ready and equipped to accelerate the development of our products and make a true innovative impact on the way cells are grown. (greenqueen.com.hk)
  • Donors grown in batch culture retained conjugative competence following signal removal, even when in stationary phase. (illinois.edu)
  • The design features an artificial filtration unit to replace the cells damaged in end-stage renal failure, because functional filtration cells are not feasibly grown in culture. (vumc.org)
  • Cells grown in culture simply do not function the same as cells taken from human kidneys, a feature common to almost all stem cells. (vumc.org)
  • The cells are grown in a specialized framework that supplies them with critical growth factors so that scientists can observe how gravity affects growth and specialization as cells become new lung tissue. (nasa.gov)
  • There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
  • While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
  • There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
  • The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
  • Adherent cultures can be further divided into 2D and 3D cultures. (integra-biosciences.com)
  • According to Christian Kaisermayer, Ph.D., project manager for cell culture at GE Healthcare Life Sciences the two significant drivers are process flexibility and complete solutions. (genengnews.com)
  • 1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)
  • IQ Products is a manufacturer of high quality, ready-to-use antibodies and antibody combinations for the detection of Human Leukocyte Antigens, Human MHC Antigens, Human Cytokines, Human Fetal Erythrocytes, Apoptosis, T cell responses and infectious diseases. (iqproducts.nl)
  • The cells can't adhere to the walls due to the continuous stirring, so spheroids are formed. (integra-biosciences.com)
  • Unlike traditional batch technologies in the sector, the startup has developed a continuous cell culture system that enables high yields at greater cost efficiencies, which has opportunities for large scale applications in the cultivated meat industry. (greenqueen.com.hk)
  • Their novel approach, which is a continuous cell culturing system, enables higher yields with lower input requirements, while also lowering the environmental footprint of manufacturing compared to traditional batch technologies. (greenqueen.com.hk)
  • We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
  • It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
  • For the quality assurance you need for molecular biology and cell culture, Corning ® Costar ® Stripette ® or KIMAX ® 51 serological pipettes are hard to beat. (sigmaaldrich.com)
  • Advanced degree (PhD, PharmD, or MS) and human T cell biology experience required. (affyimmune.com)
  • Primary neuronal culture is the possibility to visually identify neurons, characterize in details both action potentials and underlying ionic currents and to correlate electrophysiology and molecular biology on the same batch of neurons. (sav.sk)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • These unusual findings suggest that this method for abrupt change in growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell. (fit.edu)
  • Although bacterial, yeast, and insect cell expressions systems are capable of overexpressing recombinant proteins, mammalian cells are most commonly used for biologics production due to their ability to propagate human viruses, express monoclonal antibodies, and incorporate post-translational modifications such as glycosylation that are critical to the production of effective biologics. (sigmaaldrich.com)
  • Ammonium sulphate and ammonium nitrate at a concentration of 1 g/L served as the best nitrogen sources for bacterial growth, allowing B. subtilis and E. coli cells to accumulate PHA up to 62.21 and 58.7%, respectively. (scielo.org)
  • High cell density cultivation (HCDC) is developed for the production of microbial biomasses and their products. (pfeiffertheface.com)
  • What is high cell density cultivation? (pfeiffertheface.com)
  • High-cell-density cultivation (HCDC) is required to improve microbial biomass and product formation substantially. (pfeiffertheface.com)
  • On the subject of integration and "complete solutions," GE Healthcare's ReadyToProcess™ product line covers all unit operations from media preparation and cell cultivation to protein purification. (genengnews.com)
  • Advances in gene editing such as CRISPR-Cas systems further enhance production efficiency and improve product quality through modification of host cell genomes to manipulate cell growth and apoptosis, and to promote post-translational modification of the gene product. (sigmaaldrich.com)
  • Mast cells play an important role in a variety of biological processes and actively participate in the inflammatory response. (bvsalud.org)
  • Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)
  • Prior to joining Sartorius, in 2001, he began his involvement with the identification and development of integrated automated systems in cell culture, protein purification and vial filling including the Ambr® products. (b3cnewswire.com)
  • Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
  • Cell and Gene Therapy is a technique that modifies a person's genes to treat or cure disease. (ncbionetwork.org)
  • Depending on the species of the serum, each batch of serum is tested for adventitious viruses using cell culture techniques. (biowest.net)
  • In his present investigation he used this serum-free aggregating brain cell cultures as a reference culture system. (forschung3r.ch)
  • To avoid the use of serum in culture media, two approaches are currently being employed. (forschung3r.ch)
  • Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
  • The further identification of serum components that are obviously required by cultured cells is of less high priority. (forschung3r.ch)
  • It appears reasonable to assume that these particular serum components would be required by all cell types. (forschung3r.ch)
  • Knowing that lipids, and particularly cholesterol, are absent from most conventional culture media, the working hypothesis of the present investigation was that the LPs represented the still undefined serum component(s) required for cell culture. (forschung3r.ch)
  • For instance, the batch-to-batch variability has decreased as a result of the application of serum-free and chemically controlled media for bioproduction. (researchnester.com)
  • For example, in humans variability in cell density among cells of a given cell type is 100 times smaller than variation in cell mass. (pfeiffertheface.com)
  • The one that has arguably received the most attention from industry are what have been referred to as "naïve" EVs, or unmodified EVs produced from stem cells. (corning.com)
  • 7. What about stem cells? (vumc.org)
  • Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. (biomedcentral.com)
  • Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
  • The most commonly used type of stem cells, mesenchymal stem cells (MSCs), can be easily isolated from bone marrow or synovium and cultured in vitro . (openorthopaedicsjournal.com)
  • Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
  • Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
  • Aside from cell-based meat, the company's technology can be applied in the cell therapy, pharmaceutical, medical and biologics fields, but co-founders Martina Miotto, Che Connon and Leo Groenewegen believe that cultured meat is where the real opportunity for growth lies. (greenqueen.com.hk)
  • Cultured cells are used for the manufacturing of biologics such as vaccines, therapeutic proteins and antibodies, and cell therapy products. (sigmaaldrich.com)
  • Culture medium optimization is another critical component in the development of cell culture processes for the manufacturing of biologics. (sigmaaldrich.com)
  • The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. (sigmaaldrich.com)
  • Her chief research interest is the development of viral-vectored vaccines that work by inducing strong and protective T and B cell responses. (isv-online.org)
  • Immunological techniques: Immunodiffusion, immunoelectrophoresis, RIA and ELISA. (entrance-exam.net)
  • Although the term analytical in PAT is broadly defined to include chemical, physical, microbiological, mathematical, and risk analysis conducted in an integrated manner (see Table I), the emphasis in this article is on analytical techniques that enable the monitoring of critical performance attributes of raw and in-process materials and processes during biotechnology manufacturing. (biopharminternational.com)
  • However, it is important to understand that the goal of PAT is not only the use of these analytical techniques for monitoring, but also to control the manufacturing process to consistently yield the desired product quality. (biopharminternational.com)
  • We're trying introduce analytical techniques that are faster and can be done inline or at-line during manufacturing - decreasing manufacturing time and increasing the rate of product release," Carbonell says. (unc.edu)
  • Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
  • The bioassay was performed by dissolving the compounds in dimethyl sulfoxide and impregnating filter paper disks which were placed onto Mueller Hinton agar plates spread inoculated in a standardized manner to obtain even cell lawns after incubation for 18±1 hours at 37°C. Zones of growth inhibition were measured with the aid of electronic calipers. (okstate.edu)
  • Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
  • Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)
  • Numerous improvements have been made in the production and growth of cell culture medium. (researchnester.com)
  • A collection of experiment cassettes is used to accommodate experiments in cell technology, multiphase fluids, solution chemistry, separation science, microencapsulation, and crystal growth. (nasa.gov)
  • Also know asexual reproduction: Budding-a new organism develops from an outgrowth or bud on another one.Examples: Hydra Binary fission-Cell splits and produces two separate cells.Examples: Bacteria Regeneration is the process of renewal, restoration, and growth. (gnfchurch.net)
  • Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. (biomedcentral.com)
  • Research has shown the importance of growth factors in guiding and modulating the differentiation of MSCs in order to obtain the required cell type. (openorthopaedicsjournal.com)
  • Appia Bio is seeking a motivated Sr. Research Associate or Research Associate II to be part of the Process Design team with previous cell culture experience. (berkeley.edu)
  • They will be working in a highly collaborative environment to develop an innovative process for a manufacturable cell therapy product. (berkeley.edu)
  • Cell culture refers to the process of growing cells under controlled conditions outside their natural environment. (integra-biosciences.com)
  • Decreases in TraR activity and conjugative competence could be accounted for by dilution associated with cell division, suggesting that while induction of Ti plasmid conjugation is an active process, the cells lack a mechanism for disassembling the conjugative apparatus when signals become limiting. (illinois.edu)
  • In the following subsections, we briefly review some of the commonly used process analysis techniques in the biotech industry (5). (biopharminternational.com)
  • We are looking to recruit a Senior/Principal Scientist Manufacturing Science & Technology to lead a team of cell process subject matter experts (SMEs), providing process stewardship, technical support and connectivity between process development and manufacturing sites. (bios.community)
  • To gain pluripotency, the reprogramming process typically involves transfecting adult somatic cells with certain pluripotency markers. (biomedcentral.com)
  • Commercializing a cell therapy is a complex process compared with other treatments, especially when it comes to supply chain needs. (amerisourcebergen.com)
  • For titrations and analytical chemistry techniques, the BRAND ® BLAUBRAND ® ETERNA volumetric pipettes are supplied with original batch certifications. (sigmaaldrich.com)
  • AffyImmune's Tune & Track platform finely tunes the affinity of CAR T cells to reduce toxicity and increase CAR T cell longevity while allowing in vivo monitoring through a proprietary tracking system. (affyimmune.com)
  • Excitability will be investigated in primary culture of hippocampal neurons by patch clamp and in situ by in vivo electrophysiology. (sav.sk)
  • In addition there is a lack of information regarding the presence of mast cells in dental pulp of rats, which are animals used to study important aspects of the pathophysiology of dental pulp in vivo 6 . (bvsalud.org)
  • The residual haemoglobin concentration in each batch is determined by a quantitative and colorimetric assay to verify that the proper collection and processing procedures have been followed. (biowest.net)
  • Immobilization technique improved the concentration to 1350 μg/ml higher than that produced from free cells cultures and could be maintained for longer than 17 days in a repeated batch system. (scielo.org)
  • Each final product batch of sera are tested for Mycoplasma. (biowest.net)
  • The sera are tested for the absence of Mycoplasma using a cell culture assay in Axcell Biotechnologies media by culture method. (biowest.net)
  • Experience with analytical method development and validation for a variety of cell-based bioassays, including potency, mycoplasma, replication competent lentivirus, vector copy number, and sterility. (affyimmune.com)
  • The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
  • Forced-floating: a cell suspension is loaded into the wells of a low adhesion polymer-coated microplate. (integra-biosciences.com)
  • Hanging drop: a cell suspension is loaded into the wells of a hanging drop plate. (integra-biosciences.com)
  • We offer you conjugation of a volume as little as 1 mg of antibody up to large-scale single batches and the purification of the conjugates when necessary. (iqproducts.nl)
  • In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells. (sigmaaldrich.com)
  • Because EVs are much smaller than whole cells, they are able to cross biologic barriers with ease, in contrast to the relative mass of whole cells or the immunogenic responses triggered by certain synthetic products. (corning.com)
  • CellulaREvolution's technology is different from existing traditional batch-based cell culturing techniques, which are not able to meet the demand for cells as demand from various industries continues to grow - especially in the cultivated meat sector, where food techs are racing to launch their cell-based proteins on the market following the world's first regulatory approval for commercial sale in Singapore secured by Eat Just last year. (greenqueen.com.hk)
  • Their proteins are properly folded, free from contaminating related TGF beta family proteins (unfortunately often present in mammalian protein expression systems) and they include full QC data for every batch - mass spec, analytical reverse phase, recovery, SDS-PAGE, bioassay data and endotoxin testing. (o2hventures.com)
  • Fibrotic scar model and TGF-b1 differently modulate action potential firing and voltage-dependent ion currents in hippocampal neurons in primary culture. (sav.sk)
  • These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
  • It usually doesn't matter because agitation intensity can be adapted to the robustness of the cell line. (genengnews.com)
  • According to Daniel Giroux, vp of R&D, duplicating the agitation mechanisms of small reactors in large systems is problematic without subjecting cells to significant shear damage. (genengnews.com)
  • Fluorescence activated cell sorting (FACS) enables the selection of particular strains of microalgae and subsequent isolation [ 16 ]. (biomedcentral.com)
  • The characterization of different populations within any mixture of cells is performed through direct measurement of optical cell properties (for example, light scatter and multicolor fluorescence emission), which in turn enables FACS of defined cell populations that can be cultured separately at a later stage [ 17 - 19 ]. (biomedcentral.com)
  • In order to reduce morbidity associated with STSG harvesting, researchers have developed autologous dermo-epidermal substitutes (DESs) using cell culture, tissue engineering, and, more recently, bioprinting approaches. (frontiersin.org)
  • Using the latest bioengineering techniques, the Lung Tissue experiment cultures different types of lung cells in controlled conditions onboard the ISS. (nasa.gov)
  • Newer techniques using tissue engineering to regenerate musculoskeletal tissue by using biomimetic materials are now being studied. (openorthopaedicsjournal.com)
  • Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
  • The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)
  • Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
  • Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
  • Experience working in cGMP development and/or cell therapy product manufacturing is highly desirable. (bios.community)
  • With your analytical development, vendor management, and cell therapy expertise, you will leave an indelible mark on Affy. (affyimmune.com)
  • Prior oncology, cell therapy, and/or CAR T experience preferred. (affyimmune.com)
  • AffyImmune is realizing the potential of cancer immunotherapy by extending the anti-cancer activity of CAR T cell therapy to solid tumors. (affyimmune.com)
  • Biochemical separation techniques and characterization: ion exchange, size exclusion and affinity chromatography, electrophoresis, UV-visible, fluorescence and Mass spectrometry. (entrance-exam.net)
  • Then, we constructed a fibrin hydrogel-based in vitro bioassay system for the simultaneous detection of skin permeability to and pro-inflammatory activity mediated by zinc ion released from NPs by using a stable reporter cell line and a fibrin hydrogel layer. (bvsalud.org)
  • However, PERV was detected in 1 of the 3 experimental replicates of HEK293 cells cultured with encapsulated PK15 cells. (bvsalud.org)
  • Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
  • Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes. (sigmaaldrich.com)
  • If you can really improve the stability of the cells post-thawing or in a fresh state, then these issues could be addressed, extending the shelf life of fresh batches and helping to alleviate the issues associated with cryostorage. (amerisourcebergen.com)