Asepsis

Encyclopedias as Topic

Asepsis

Genotoxicity

Halobacterium noricense

Endospore

Microbial art

Indicator bacteria

Edward Bach

Biomolecular engineering

Silver nanoparticle

Bioclogging

Mantoux test

Community fingerprinting

Pulsed-field gel electrophoresis

Phage typing

Biogenic substance

Bacterial display

Janet Gilsdorf

Asymmetric bacterium

Multilocus sequence typing

Clostridioides difficile infection

Philippe Horvath

Xenorhabdus ehlersii

List of MeSH codes (E05)

Porter W. Anderson Jr.

Protoplast

Human Microbiome Project

Salami

Stimulus-triggered acquisition of pluripotency

Agar dilution

Maple syrup

Pneumonia

Strains11

• Phylogenetic trees based on 16S rRNA gene sequences revealed that strain YSS-7(T) clustered with the type strains of Pelagicola litoralis, Planktotalea frisia, Pacificibacter maritimus and Roseovarius marinus. (nih.gov)
• Strain YSS-7(T) exhibited the highest 16S rRNA gene sequence similarity (97.7%) to the type strain of Pelagicola litoralis and sequence similarity of more than 96.0% to the type strains of some other species. (nih.gov)
• The fatty acid and polar lipid profiles of strain YSS-7(T) were different from those of the type strains of phylogenetically related species. (nih.gov)
• Computer analysis of 1-dimensional and 2-dimensional protein patterns to establish databases of pathogenic bacteria, biotechnologically important bacteria and eucaryotic cells provides methods for screening and identifying bacterial strains, recognizing new groups, and characterizing human and animal disease related protein abnormalities. (europa.eu)
• Typing of isolates is useful during outbreaks to determine the prevalence and mode of spread of strains and to identify reservoirs and carriers. (isid.org)
• An important limitation of current infection control methodology is that the available bacterial typing methods cannot distinguish between different strains of MRSA. (sanger.ac.uk)
• Current clinical methods to make links between related strains compare the pattern of bacterial susceptibility to a profile of antibiotics. (sanger.ac.uk)
• A unique method to screen large-scale libraries for industrially useful bacterial strains was recently developed by Tokyo Tech researchers. (titech.ac.jp)
• Nonetheless, the traditional method of genetically engineering bacterial strains for high protein production is extremely time consuming. (titech.ac.jp)
• The study was led by Associate Professor Tetsuya Kitaguchi from Tokyo Institute of Technology (Tokyo Tech), Japan, and was conducted in collaboration with Ajinomoto Co., Inc. The innovative method, which combines microfluidics and versatile biosensing to quickly identify enhanced bacterial strains that produce the highest amount of a desired protein, is reported in their study published in the journal Small on April 24, 2023. (titech.ac.jp)
• The researchers put their method to the test by screening a huge library of bacterial strains created to produce FGF9, a human cytokine, and subjected to circumstances that cause random mutations. (titech.ac.jp)

Infections5

• Although considered the gold-standard for diagnosing bacterial infections this process is time-consuming, taking several days to produce the results, and requires specialist equipment and expert laboratory personnel. (birmingham.ac.uk)
• This will decrease the time taken to identify and deliver beneficial treatments to patients, and therefore improve the treatment of bacterial infections. (birmingham.ac.uk)
• Every lesional, itchy animal should be evaluated for secondary bacterial and Malassezia skin and ear infections , using standard procedures (i.e., impression smears, tape preparations). (vin.com)
• Acetate tapes are only to be used when yeast infections are suspected as the background material on the Scotch tape makes bacterial evaluation difficult, especially cocci. (vin.com)
• Bacterial infections may develop slowly and tend to recur (chronic bacterial prostatitis) or develop rapidly (acute bacterial prostatitis). (msdmanuals.com)

Infection15

• Pericardial fluid Gram stain is a method of staining a sample of fluid taken from the pericardium to diagnose a bacterial infection. (medlineplus.gov)
• The Gram stain method is one of the most commonly used techniques to rapidly identify the type of bacteria causing the infection. (medlineplus.gov)
• Blood tests and bacterial culture can help identify the specific organism causing the infection. (medlineplus.gov)
• Secondary bacterial infection is common, and lesions may cause increased pain and disability and fatal septicaemia (severe infection involving the entire human system) if untreated. (who.int)
• Depending on your family history, your doctor may take a swab to check for specific bacterial infection. (uvahealth.com)
• In a new study released today in New England Journal of Medicine , researchers demonstrate that whole-genome sequencing can provide clinically relevant data on bacterial transmission within a timescale that can influence infection control and patient management. (sanger.ac.uk)
• Fast and accurate detection of bacterial transmission is crucial to better control of healthcare-associated infection. (sanger.ac.uk)
• The Department of "Computational Biology for Infection Research" studies the human microbiome, viral and bacterial pathogens, and human cell lineages within individual patients by analysis of large-scale biological and epidemiological data sets with computational techniques. (helmholtz-hzi.de)
• Both papers--one published online July 28, 2005 in Science Express and one in the August issue of Infection and Immunity --focus on aspects of the type-III pathway, a molecular syringe that Yersinia pestis , the bacterium that may have killed more people throughout history than any other infectious disease, uses to disable its host's immune system. (uchicagomedicine.org)
• The technique can be used to identify whether the bacteria causing an infection such as MRSA is resistant to certain drugs and antibiotics, and therefore identify treatments most likely to be effective. (birmingham.ac.uk)
• Currently, when a patient presents with a bacterial infection, the infection is diagnosed by taking a sample from the patient, growing bacteria from it and then using genetic sequencing to identify the type of bacteria present and the presence of markers of resistance. (birmingham.ac.uk)
• The cause is sometimes a bacterial infection. (msdmanuals.com)
• Bacterial infection is treated with antibiotics. (msdmanuals.com)
• Some people develop chronic prostatitis in the absence of bacterial infection. (msdmanuals.com)
• Occasionally, prostatitis without bacterial infection causes inflammation but no symptoms. (msdmanuals.com)

Species8

• Author Summary Almost all bacterial species use thin surface appendages called pili to interact with their environments. (biorxiv.org)
• Databases were established for identification of bacterial species and protein types within species and standard 2-dimensional methodology was applied to human, murine, bacterial and streptomycete cells. (europa.eu)
• They also find that nanopore sequencing signals display complex heterogeneity across methylation events of the same type, which suggests that detection methods are best developed using a diverse collection of species. (genomeweb.com)
• To enable nanopore sequencing for broad methylation discovery, they generated a training dataset from an assortment of bacterial species and develop a technique that combines the identification and fine mapping of the three forms of methylation into a multi-label classification framework. (genomeweb.com)
• We have demonstrated that SESI-MS volatile fingerprinting, combined with a statistical analysis method, can be used to differentiate bacterial genera, species, and mixed cultures in a variety of growth media. (jove.com)
• Certain species of yeast and bacteria have to be identified to produce novel, differentiated beer types and consistent brewing recipes. (brewlab.co.uk)
• Small intestinal bacterial overgrowth (SIBO) consists of increased and/or abnormal microbiota comprising different bacterial species derived from the colonic flora that ascend to small intestine segments and may be associated with a myriad of gastrointestinal symptoms and extraintestinal complications 1 1. (scielo.br)
• combined with metagenomics has uncovered thousands of novel bacterial species directly from samples without isolation or culturing. (helmholtz-hzi.de)

Isolates4

• Molecular typing by pulsed field gel electrophoresis showed that all isolates were closely related. (cdc.gov)
• Comparison ("typing" or "fingerprinting") of epidemiologically related isolates helps to determine whether these organisms are related or not and thus essential to confirm the existence of an outbreak. (isid.org)
• Current laboratory techniques often cannot distinguish between MRSA isolates. (sanger.ac.uk)
• In an analysis of 292 bacterial isolates, the device was able to detect between 20 to 100 genome types. (hcplive.com)

Bacteria7

• Until the late 19th century, physicians rejected the connection between Louis Pasteur's germ theory that bacteria caused diseases and antiseptic techniques. (wikipedia.org)
• Type IV pili are ubiquitous surface appendages in Gram-negative bacteria that promote diverse activities including attachment, virulence, biofilm formation, horizontal gene transfer, and twitching motility [ 1 - 5 ]. (biorxiv.org)
• An approach for using nanopore sequencing to discover different types of DNA methylation from bacteria and the microbiome is described by a Mount Sinai-led team in this week's Nature Methods . (genomeweb.com)
• For bacterial mycetoma (actinomycetoma) treatment relies on long-term treatment with a combination of antibiotics, tailored to the type of bacteria involved. (who.int)
• Lactobacilli and their antimicrobial and anti-inflammatory products along with components of the epithelial mucosal barrier provide an effective first line defense against invading pathogens including bacterial vaginosis, aerobic vaginitis-associated bacteria, viruses, fungi and protozoa. (frontiersin.org)
• This technique enabled the team to identify the cell types targeted by the bacteria. (uchicagomedicine.org)
• A new technique developed by scientists in the Physical Sciences for Health Centre at the University of Birmingham can identify the resistance profile of bacteria in a much shorter time than traditional diagnostic methods. (birmingham.ac.uk)

Microbiology1

• Important progress made in the fields of instruments, reagents and techniques have make it easier to adapt to the important changes of the clinical microbiology context e.g. increasing use of microbiology tests, shortage of qualified personnel. (isid.org)

Method for bacterial identification2

• We are expanding the use of SESI-MS to the detection of bacterial volatiles as a method for bacterial identification and characterization 8 . (jove.com)
• Recently, scientists have questioned if cultures are indeed the reference method for bacterial identification 1,3-4,7 . (bvsalud.org)

Genes2

• In the paper, a team led by Broad Institute scientists use their activity-by-contact model, which predicts which enhancers regulate which genes, to create enhancer-gene maps in 131 human cell types and tissues, then apply the maps to analyze fine-mapped genetic variants associated with 72 diseases and complex traits. (genomeweb.com)
• Among their findings are new genes and pathways related to inflammatory bowel disease, hundreds of genes that appear to control different traits through effects in different cell types, and a role for the mitochondrial metabolism-related gene PPIF in tuning mitochondrial function in macrophages. (genomeweb.com)

Composition1

• While bacterial community composition recovered using Illumina 16S rRNA amplicon sequencing was not detectably biased by any method, the quantity of bacterial DNA varied drastically, reducing the number of samples that could be amplified and sequenced. (harvard.edu)

Anti-bacterial1

• The anti-bacterial effect of different antibiotics and EOs (thyme, oregano, lemongrass, mint, and rosemary) was determined using the standard disc diffusion method. (scielo.br)

Molecular3

• Her research has involved using molecular, cell and computational biology tools and techniques to understand the pathological mechanisms underlying neurological disorders. (technologynetworks.com)
• This service applies cutting edge molecular biology techniques, allowing you to identify any particular bacterial or yeast strain so you can optimise recipe formulation and brewhouse efficiency. (brewlab.co.uk)
• Molecular typing of Mycobacterium spp. (who.int)

Cultures3

• This includes medical and laboratory techniques (such as with bacterial cultures). (wikipedia.org)
• These tiny droplets were used as microscopic bacterial cultures and reactors. (titech.ac.jp)
• The proposed method involves using water microdroplets as tiny bacterial cultures and bioreactors and then sorting them based on their fluorescence intensity. (titech.ac.jp)

Yeast2

• Using industry standard microscopy techniques, this service quantifies yeast cell count of your sample as part of your regular quality control (QC) procedures. (brewlab.co.uk)
• Using industry standard microscopy techniques, this service determines the viability of your yeast and its suitability for brewing. (brewlab.co.uk)

Strain6

• A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain, designated YSS-7(T), was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea of South Korea. (nih.gov)
• nov. is proposed, with 200901116(T) ( = CIP 110703(T) = DSM 28999(T)) as the type strain. (nih.gov)
• This service applies microbiological techniques to genetically identify typical strain characteristics, allowing you to optimise fermentation processes and flavour production. (brewlab.co.uk)
• the type strain is 656.84 T (5DSM 23593 T 5CIP 101062 T). (hal.science)
• To this end, the researchers first used a type of biosensor called Q-body to measure the amount of the desired protein produced by each strain. (titech.ac.jp)
• Thus, fluorescence intensity is directly related to the amount of the desired protein that each bacterial strain can produce. (titech.ac.jp)

Microbial1

• The recent development of methods applying next-generation sequencing to microbial community characterization has led to the proliferation of these studies in a wide variety of sample types. (harvard.edu)

Fungal2

• Mycetoma has two distinct etiologies: fungal and bacterial. (who.int)
• For the fungal type (eumycetoma), treatment is based on administration of antifungal agents for prolonged periods, usually preceded or followed by surgical excision of the lesions. (who.int)

Protein6

• However, the function of this protein has remained unclear because pilU mutants exhibit inconsistent phenotypes among type IV pilus systems and because it is relatively understudied compared to PilT. (biorxiv.org)
• Type IV pili are composed almost exclusively of a single protein called the major pilin, which forms a helical fiber that extends from the cell surface [ 6 ]. (biorxiv.org)
• 1-dimensional and 2-dimensional electrophoretic techniques provide powerful tools for the analysis of complex protein mixtures. (europa.eu)
• Total protein is the sum of all types of proteins in the pleural fluid and may be reported as the concentration of protein in the sample. (testing.com)
• Lactate dehydrogenase is a type of protein called an enzyme. (testing.com)
• NT-proBNP is a type of protein that is associated with heart function. (testing.com)

Cells6

• Impact of bacterial and somatic cells content on quality fresh milk in small-scale dairy farms in Kosovo. (scielo.br)
• And so we work to enhance our interrogation techniques, for while extracting information from a biological system is challenging, it is also indispensable to a range of missions: revealing how cells organize into tissues, dissecting disease pathogenesis, and advancing diagnostic and treatment efforts. (genengnews.com)
• Part of the discrepancy may be ascribed to differences in the viability of the starting cells, in eliciting factors, in separation techniques and in storage conditions. (stanford.edu)
• Vaccines of this type, prepared from suspensions of inactivated Bordetella pertussis bacterial cells, have been licensed for routine vaccination of infants since the mid-1940s. (cdc.gov)
• This analysis determines the total number of cells in the sample and can also include a cell count differential, which calculates the amounts of each cell type that is present. (testing.com)
• Triglycerides are a type of fat that is generated when extra calories are stored in cells to be used for future energy needs. (testing.com)

Diagnostic1

• as well as diagnostic techniques, drug development and tissue transplantation. (who.int)

Characterization4

• We report here the first isolation and characterization of a cluster of VanA-type glycopeptide-resistant E. faecium in a teaching hospital in Colombia. (cdc.gov)
• This protocol provides instructions for the rapid (within minutes) characterization of bacterial VOCs using SESI-MS. (jove.com)
• 8 Here we provide the steps for obtaining bacterial volatile fingerprints using SESI-MS, including the instrumental parameters that should be optimized to ensure robust bacterial identification and characterization. (jove.com)
• As a demonstration of the use of SESI-MS for the characterization of bacterial volatiles, E. coli K12 and P. aeruginosa PAO1 are cultured aerobically for 24 h in 50 mL LB-Lennox at 37 °C and the SESI-MS spectra of the headspace volatiles are collected in 2 minutes. (jove.com)

Commonly1

• In this study, we examine the efficacy of several commonly used protocols for extracting bacterial DNA from ants. (harvard.edu)

Microorganisms2

• The second type of evidence-showing the potential for microorganisms to degrade the contaminants-is also relatively simple to provide. (nationalacademies.org)
• Endospores, or bacterial spores, are the microorganisms primarily used in BIs. (scienceoxygen.com)

Inflammation1

• This type may or may not involve inflammation. (msdmanuals.com)

Methods1

• The investigators note that existing methods for detecting DNA modification from nanopore sequencing data cannot effectively characterize unknown bacterial methylomes without previous knowledge. (genomeweb.com)

Lesions1

• This sampling technique is mainly used for fluid-containing lesions. (vin.com)

Vibrio1

• Here, we study the type IVa competence pilus of Vibrio cholerae as a model system to define the role of PilU. (biorxiv.org)

High-throughput1

• This problem could be significantly mitigated by coordinately screening multiple tissue types simultaneously in a high-throughput fashion during upstream tier 1 screening. (inknowvation.com)

Excess1

• Excess carrier gas and unreacted bacterial volatiles are passed through a 0.22 μm filter (5), as an additional measure of protection, and vented to a chemical hood. (jove.com)

Identification1

• The time taken to make the identification is currently less than a day, however with further development and streamlining of the technique this will be further reduced. (birmingham.ac.uk)

Crystallography2

• Neutron crystallography, which analyzes diffraction from atomic nuclei in the enzyme (all atoms have an atomic nucleus), is an alternative imaging technique that the researchers chose for their work. (sciencedaily.com)
• The drug discovery company Implexion Pharma and researchers from Lund University have explored new potential drug candidates for type 2 diabetes using X-ray crystallography research techniques at MAX IV. (lu.se)

Genetic1

• 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

Parameters3

• The specimens were analyzed regarding surface roughness parameters (Ra, Rz, Rq), sterilized and subjected to bacterial adhesion. (unicamp.br)
• The type of material and surface finish system significantly interfered with surface roughness parameters and bacterial adhesion. (unicamp.br)
• 4A Type 4 Steam Chemical Indicators are the indicators used inside sterilization packages during the steam sterilization and when multi parameters are obtained, the color turns into its referance color. (scienceoxygen.com)

Occasionally1

• In children, primitive neuroectodermal tumors are the most common cause of leptomeningeal carcinomatosis, but pilocytic astrocytomas (which are generally low grade) occasionally can demonstrate this type of aggressive behavior. (medscape.com)

Filters1

• Each laboratory was randomly allocated 14 filters per dust type. (cdc.gov)

Diagnose1

• Another technique brought up in the presentation to diagnose VAP was the rapid automated microscopy, mini bronchoalveolar lavage (mini-BAL). (hcplive.com)

Biological2

• Bacterial type IV pili are critical for diverse biological processes including horizontal gene transfer, surface sensing, biofilm formation, adherence, motility, and virulence. (biorxiv.org)
• How many types of biological indicators are there? (scienceoxygen.com)

Microscopy2

• IHC, in combination with microscopy and image analysis techniques, has become a very powerful tool that offers a direct visualization of tissue antigens using labeled antibodies specific to the antigen. (technologynetworks.com)
• The tissue samples are prepared following specialized techniques to enable the entry of the antibodies, and the label is detected using light or electron microscopy (Figure 2). (technologynetworks.com)

MRSA1

• Work conducted by PhD student Nathaniel Wand has proven that the DNA barcoding technique is able to identify DNA fragments in a mixed sample, containing segments such as those which provide a marker of resistance in MRSA, which would be very beneficial to identify in a healthcare setting. (birmingham.ac.uk)

Analysis3

• Computer systems of 2-dimensional gel analysis namely PDQUEST and BioImage were implemented and databases were established for the studied cell types. (europa.eu)
• Although laboratory and analysis techniques should be standardized across diverse sample types as much as possible, minimal modifications such as these will increase the number of environments in which bacterial communities can be successfully studied. (harvard.edu)
• Schematic for SESI-MS analysis of bacterial volatiles. (jove.com)

Sterilization3

• What are the two types of sterilization indicators? (scienceoxygen.com)
• What are 3 types of sterilization? (scienceoxygen.com)
• Chemical indicators should be used in all types of sterilization processes including: steam, hydrogen peroxide, and ethylene oxide. (scienceoxygen.com)

Enzyme3

• However, this technique obtains structural information by diffraction from electrons in the enzyme. (sciencedaily.com)
• Amylase is a type of enzyme involved in the digestion of carbohydrates. (testing.com)
• The ImP is produced by the bacterial enzyme urocanate reductase (UrdA). (lu.se)

Search2

• Search When typing in this field, a list of search results will appear and be automatically updated as you type. (prweb.com)
• In the search for a new, potential drug candidate for type 2 diabetes treatment, these new findings guided Implexion Pharma in their target selection for such a candidate during a research project in 2020-2021. (lu.se)

Human2

• The use of the technique of nuclear transfer for reproduction of human beings is surrounded by strong ethical concerns and controversies and is considered a threat to human dignity. (who.int)
• This technique is surrounded by strong ethical concerns and is considered a threat to human dignity. (who.int)