Bacterial Proteins: Proteins found in any species of bacterium.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Peptostreptococcus: A genus of gram-positive, anaerobic, coccoid bacteria that is part of the normal flora of humans. Its organisms are opportunistic pathogens causing bacteremias and soft tissue infections.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Rumen: The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)Abomasum: The fourth stomach of ruminating animals. It is also called the "true" stomach. It is an elongated pear-shaped sac lying on the floor of the abdomen, on the right-hand side, and roughly between the seventh and twelfth ribs. It leads to the beginning of the small intestine. (From Black's Veterinary Dictionary, 17th ed)Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Periplasm: The space between the inner and outer membranes of a cell that is shared with the cell wall.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genes, Bacterial: The functional hereditary units of BACTERIA.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Peptide Elongation Factor Tu: A protein found in bacteria and eukaryotic mitochondria which delivers aminoacyl-tRNA's to the A site of the ribosome. The aminoacyl-tRNA is first bound to a complex of elongation factor Tu containing a molecule of bound GTP. The resulting complex is then bound to the 70S initiation complex. Simultaneously the GTP is hydrolyzed and a Tu-GDP complex is released from the 70S ribosome. The Tu-GTP complex is regenerated from the Tu-GDP complex by the Ts elongation factor and GTP.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Host-Pathogen Interactions: The interactions between a host and a pathogen, usually resulting in disease.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Protein Synthesis Inhibitors: Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Legionella pneumophila: A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.Bacterial Secretion Systems: In GRAM NEGATIVE BACTERIA, multiprotein complexes that function to translocate pathogen protein effector molecules across the bacterial cell envelope, often directly into the host. These effectors are involved in producing surface structures for adhesion, bacterial motility, manipulation of host functions, modulation of host defense responses, and other functions involved in facilitating survival of the pathogen. Several of the systems have homologous components functioning similarly in GRAM POSITIVE BACTERIA.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Protein Sorting Signals: Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Flagellin: A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Salmonella typhimurium: A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.Thiostrepton: One of the CYCLIC PEPTIDES from Streptomyces that is active against gram-positive bacteria. In veterinary medicine, it has been used in mastitis caused by gram-negative organisms and in dermatologic disorders.Prokaryotic Initiation Factor-2: The largest of the three prokaryotic initiation factors with a molecular size of approximately 80 kD. It functions in the transcription initiation process by promoting the binding of formylmethionine-tRNA to the P-site of the 30S ribosome and by preventing the incorrect binding of elongator tRNA to the translation initiation site.Yersinia pseudotuberculosis: A human and animal pathogen causing mesenteric lymphadenitis, diarrhea, and bacteremia.Chloramphenicol: An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Digestion: The process of breakdown of food for metabolism and use by the body.Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Peptides, Cyclic: Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Eukaryotic Cells: Cells of the higher organisms, containing a true nucleus bounded by a nuclear membrane.Bacillus subtilis: A species of gram-positive bacteria that is a common soil and water saprophyte.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Toll-Like Receptor 5: A pattern recognition receptor that binds FLAGELLIN. It mediates cellular responses to certain bacterial pathogens.Oxazolidinones: Derivatives of oxazolidin-2-one. They represent an important class of synthetic antibiotic agents.Eukaryota: One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Proteome: The protein complement of an organism coded for by its genome.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Kinetics: The rate dynamics in chemical or physical systems.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Bacterial Physiological Phenomena: Physiological processes and properties of BACTERIA.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Glycosyltransferases: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Acetamides: Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Ribosomes: Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.Vacuoles: Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Puromycin: A cinnamamido ADENOSINE found in STREPTOMYCES alboniger. It inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Molecular Chaperones: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.ThiazolesHydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Structural Homology, Protein: The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Salmonella Infections: Infections with bacteria of the genus SALMONELLA.Protein Folding: Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Helicobacter pylori: A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405).Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Molecular Weight: The sum of the weight of all the atoms in a molecule.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Subcellular Fractions: Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)Leucine: An essential branched-chain amino acid important for hemoglobin formation.Chlamydia trachomatis: Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Ribosomal Proteins: Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Streptomyces: A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Dietary Proteins: Proteins obtained from foods. They are the main source of the ESSENTIAL AMINO ACIDS.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.Microscopy, Electron, Transmission: Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.Mice, Inbred BALB CEpitopes: Sites on an antigen that interact with specific antibodies.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Cytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Protein Interaction Mapping: Methods for determining interaction between PROTEINS.Heat-Shock Proteins: Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Protein PrecursorsSequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Protein Structure, Quaternary: The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).Antigen Presentation: The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)Oligopeptides: Peptides composed of between two and twelve amino acids.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Mycobacterium tuberculosis: A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Sequence Deletion: Deletion of sequences of nucleic acids from the genetic material of an individual.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Dimerization: The process by which two molecules of the same chemical composition form a condensation product or polymer.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Time Factors: Elements of limited time intervals, contributing to particular results or situations.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Mice, Inbred C57BLTransfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

*  Fluorescent Protein Vectors for Cloning and Bacterial Expression, Living Colors
Validated with a wide variety of fusion proteins. ... Use Living Colors basic fluorescent protein vectors to study proteins in live cells. ... Protein Expression & Purification * Protein Learning Center * Protein Learning Center * ... Fluorescent Protein Vectors for Cloning & Bacterial Expression. These bacterial ......
http://clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins/Bacterial_Expression?sitex=10020:22372:US&PROD=CUsMEjDXoLSuHQ7e08c19Krv:S&PROD_pses=ZG9FB19F9792A3B030680176D97ACE528789FA0E3BB80579FB41742C5E29BEC6C288CC0FF609C450837DC21A25E51FD0C3B953DEC66D0CBBF4
*  Family » 1.3.16.01. General Bacterial Porin (GBP)
General Bacterial Porin (GBP) 1.B.1 (TCDB) PF00267, PDBsum (16 proteins). Protein Name. ... General Bacterial Porin (GBP) 1.B.1 (TCDB) PF00267, PDBsum. Anion-selective porin. ... Outer membrane protein C. 1osm. Klebsiella pneumoniae. Bact. Gram-neg outer. 3. 48. 24.0 ......
http://opm.phar.umich.edu/families.php?family=43
*  Stuck on you: Scientists lay bare secrets of bacterial attachmen...( An...
... bacterial,attachment,proteins,biological,biology news articles,biology news today,latest ... Scientists discover proteins involved in spread of HIV-1 infection. 8. Scientists fear ... They do this with the help of proteins on their outer surface called adhesins and ... Scientists visualise cellular handmaiden that restores shape to proteins. 11. Scientists ......
http://news.bio-medicine.org/biology-news-2/Stuck-on-you-3A-Scientists-lay-bare-secrets-of-bacterial-attachment-proteins-348-1/
*  Chlamydia Inhibit Host Cell Apoptosis by Degradation of Proapoptotic BH3-only...
This process requires bacterial protein synthesis and operates during infection with both ... The inhibition of bacterial protein synthesis with rifampin prevented the disappearance ( ... bacterial protein synthesis can be estimated by monitoring the production of bacterial ... This release is governed by the Bcl-2 family of proteins. Bcl-2 proteins can be ......
http://jem.rupress.org/content/200/7/905
*  Scientists identify molecular structure of cancer-relate...( NEW YORK December...
... proteins,biological,biology news articles,biology news today,latest biology news,current ... Stuck on you: Scientists lay bare secrets of bacterial attachment proteins. 8. Scientists ... The technique yielded the first-ever detailed pictures of the proteins interacting with ... expressed the corresponding proteins in bacteria, and then purified them '"/> ......
http://news.bio-medicine.org/biology-news-2/Scientists-identify-molecular-structure-of-cancer-related-proteins-8578-1/
*  Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7...
Core functions of autophagy are mediated by ubiquitin-like protein (UBL) cascades, in ... 24373746 - Nucleotide and partner-protein control of bacterial replicative helicase ... As with ubiquitin and SUMO E2s, Atg3 and Atg10 make numerous protein-protein interactions ... A protein conjugation system essential for autophagyNature3953958Year: 19989759731. 33. ......
http://biomedsearch.com/nih/Noncanonical-E2-recruitment-by-autophagy/23142976.html
*  British Library EThOS: The dual-targeted membrane assembly of the Streptomyces...
The Rieske protein is a membrane-bound iron-sulphur protein found in bacterial electron ... There are two major protein translocases for protein export across the bacterial ... The dual-targeted membrane assembly of the Streptomyces coelicolor Rieske protein Author: ... Previous work has shown that the Streptomyces coelicolor Rieske protein is dual targeted ......
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.699487
*  2vl0 » Proton-gated ion channel, inactive conformation 1 - Orientations of...
Localization: Bacterial Gram-negative inner membrane (475 proteins). 2vl0 » Proton-gated ... Prokaryotic pentameric ligand-gated ion channels (21 proteins) 1.A.9 (TCDB) ......
http://opm.phar.umich.edu/protein.php?pdbid=2vl0
*  3kly » Formate transporter 1, FocA - Orientations of Proteins in Membranes (OPM...
Species: Vibrio cholerae (11 proteins). *Localization: Bacterial Gram-negative inner ... Major Intrinsic Protein (MIP)/FNT superfamily (2 families) *Family: 1.1.14.02. Formate/ ... Nitrite transporter (FNT) family (5 proteins) 1.A.16 (TCDB). * ......
http://opm.phar.umich.edu/protein.php?pdbid=3kly
*  3f7y » Potassium channel KcsA, 17A opening conformer - Orientations of Proteins...
Localization: Bacterial Gram-positive plasma membrane (143 proteins). 3f7y » Potassium ... KcsA voltage-gated K+ channels (13 proteins) 1.A.1.1 (TCDB). *Species: Streptomyces ......
http://opm.phar.umich.edu/protein.php?pdbid=3f7y
*  GenProp0059
... . Genome Properties. JCVI Home. Genome Properties Home. TIGRFAMs. Genome Properties List. Top Level Genome Properties. Search CMR Genome Properties. Genome Property Definition Page Accession GenProp0059. Name type I secretion. Type SYSTEM. Description A type I protein secretion system is an ABC transporter. The system is Sec-independent. ATP drives the transport of target proteins across both inner and outer membranes and into the extracellular medium. There is no cleaved N-terminal signal sequence. Instead, the type I system recognizes C-terminal information, localized to the last 50 amino acids or less. Characteristic sequence found nearby, such as repeats of the motif GGXGXDXXX in Erwinia, may play a supporting role, possibly for chaperoning the rest of the protein rather than for recognition of the export signal reference PMID:11157948. This property does NOT cover ABC transporter-mediated processing and secretion of class II bacteriocins of certain Gram-positive bacteria, because of the following di...
http://jcvi.org/cgi-bin/genome-properties/GenomePropDefinition.cgi?prop_acc=GenProp0059
*  TIGR01842
... . TIGRFAMs. JCVI Home. TIGRFAMs Home. Genome Properties. TIGRFAMs Home. TIGRFAMs Terms. TIGRFAMs Complete Listing. TIGRFAMs FTP site. TIGRFAMs Resources. TIGR01842 Seed Alignment. HMM Summary Page: TIGR01842. Accession. TIGR01842. Name. type I sec PrtD. Function. type I secretion system ATPase. Trusted Cutoff. 510.15. Domain Trusted Cutoff. 510.15. Noise Cutoff. 386.60. Domain Noise Cutoff. 386.60. Isology Type. subfamily. HMM Length. 544. Mainrole Category. Protein fate. Subrole Category. Protein and peptide secretion and trafficking. Gene Ontology Term. GO:0008565 : protein transporter activity molecular function. GO:0030253 : protein secretion by the type I secretion system biological process. Author. Haft DH. Entry Date. Mar 3 2003 2:34PM. Last Modified. Feb 14 2011 3:27PM. Comment. Type I protein secretion is a system in some Gram-negative bacteria to export proteins often proteases across both inner and outer membranes to the extracellular medium. This is one of three proteins of the type I secreti...
http://jcvi.org/cgi-bin/tigrfams/HmmReportPage.cgi?acc=TIGR01842
*  Ferric uptake regulator family
... in molecular biology the ferric uptake regulator fur family of proteins includes metal ion uptake regulator protein s these are responsible for controlling the intracellular concentration of iron in many bacteria although iron is essential for most organisms high concentrations can be toxic because of the formation of hydroxyl radical s furs can also control zinc homeostasis and is the subject of research on the pathogenesis of mycobacteria references category protein families...
https://en.wikipedia.org/wiki/Ferric_uptake_regulator_family
*  Anti -FAM84A (Protein FAM84A, Neurologic Sensory Protein 1, NSE1, FLJ35392, PP11517) - United States
anti fam a protein fam a neurologic sensory protein nse flj pp united states biological login email password forgot your password new user remember me home distributors technical about register antibodies biochemicals culture media custom services growth factors bioassay kits lectins molecular biology serum tissues protocols newsletter about us contact you are here home antibodies antibodies fam family with sequence similarity anti fam a protein fam a neurologic sensory protein nse flj pp anti fam a protein fam a neurologic sensory protein nse flj pp pricing for pricing information usa customers sign in outside usa please contact your distributor for pricing specifications clone host grade applications monoclonal mouse affinity purified e b ih fam a family with sequence similarity member a was identified in a screen for genes up regulated in colon cancer it may play a role in cell migration and has a critical role in the progression of colon cancer catalog applications suitable for use in elisa western blot a...
http://usbio.net/item/126611
*  SMART: Trans reg C domain annotation
... SMART MODE: NORMAL GENOMIC. Schultz et al. 1998 Proc. Natl. Acad. Sci. USA 95, 5857-5864 Letunic et al. 2014 Nucleic Acids Res doi: 10.1093/nar/gku949 HOME. SETUP. FAQ. ABOUT. GLOSSARY. WHAT'S NEW. FEEDBACK. Trans reg C Transcriptional regulatory protein, C terminal. SMART accession number: SM00862. Description: This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding. Interpro abstract IPR001867 : Two-component signal transduction systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions. Some bacteria can contain up to as many as 200 two-component systems that need tight regulation to prevent unwanted cross-talk. These pathways have been adapted to response to a wide variety of stimuli, including nutrients, cellular redox state, changes in osmolarity, quorum signals, antibiotics, and more. Two-component systems are comprised of a sensor histidine kinase HK and its cognate res...
http://smart.embl-heidelberg.de/smart/do_annotation.pl?ACC=SM00862&BLAST=DUMMY
*  SMART: Trans reg C domain annotation
... SMART MODE: NORMAL GENOMIC. Schultz et al. 1998 Proc. Natl. Acad. Sci. USA 95, 5857-5864 Letunic et al. 2014 Nucleic Acids Res doi: 10.1093/nar/gku949 HOME. SETUP. FAQ. ABOUT. GLOSSARY. WHAT'S NEW. FEEDBACK. Trans reg C Transcriptional regulatory protein, C terminal. SMART accession number: SM00862. Description: This domain is almost always found associated with the response regulator receiver domain. It may play a role in DNA binding. Interpro abstract IPR001867 : Two-component signal transduction systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions. Some bacteria can contain up to as many as 200 two-component systems that need tight regulation to prevent unwanted cross-talk. These pathways have been adapted to response to a wide variety of stimuli, including nutrients, cellular redox state, changes in osmolarity, quorum signals, antibiotics, and more. Two-component systems are comprised of a sensor histidine kinase HK and its cognate res...
http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=Trans_reg_C&BLAST=DUMMY
*  SecY protein
{{Pfam box Symbol = SecY Name = eubacterial secY protein image = PDB 1rh5 EBI.jpg width = caption = Structure of a protein-conducting channel. 1 Pfam= PF00344 InterPro= IPR002208 SMART= PROSITE = PDOC00612 SCOP = 1rh5 TCDB = 3.A.5 OPM family= 19 OPM protein= 1rh5 PDB= A:69-417. The 'SecY protein ' is the main transmembrane subunit of the eubacterial Sec or Type II secretory pathway and a protein-secreting ATPase complex, also known as a translocon. Secretion of some proteins carrying a signal-peptide across the inner membrane in Gram-negative bacteria occurs via the preprotein translocase pathway. Proteins are produced in the cytoplasm as precursors, and require a chaperone subunit to direct them to the translocase component within the membrane. The translocase pathway comprises 7 proteins, including a chaperone protein SecB, an ATPase SecA, an integral membrane complex SecY, SecE and SecG, and two additional membrane proteins that promote the release of the mature peptide into the periplasm SecD and SecF. Th...
https://en.wikipedia.org/wiki/SecY_protein
*  Annual report of Biology of Cell Interactions for year 2005
The research of this unit focuses on i the mechanisms of entry, signalisation and the intracellular fate of cytokine receptors ii the mechanisms of entry and development of intracellular bacteria, the Chlamydia, which are mainly responsible for pneumopathies, sexually transmitted diseases and blindness. The research of this unit focuses on the mechanisms of entry, signalisation and the intracellular fate of cytokine receptors. Sorting of intracellular receptors : a role for ubiquitin. We have shown that IL2 receptor chains are mono-ubiquitinated, which is essential for this intracellular sorting. 2 Invasion OF HOST CELLS BY INTRACELLULAR BACTERIA, CHLAMYDIA. Chlamydiae are bacteria that proliferate only within eukaryotic host cells. Since Chlamydia are strict intracellular parasites, their development depends on their capacity of being internalized by the host cell, in particular by epithelial cells, which are their main target Figure 4. We have shown that infection by C. The inclusion membrane contains lipid...
http://pasteur.fr/recherche/RAR/RAR2005/print/Ubic-en.html
*  Category:Bacterial proteins
category bacterial proteins category bacterial proteins bacterial proteins are proteins from which any bacterium may be comprised in its natural state category proteins by type of organism category bacteriology...
https://en.wikipedia.org/wiki/Category:Bacterial_proteins
*  Which poisonous bacterial protein is best for you?
Which poisonous bacterial protein is best for you....
http://io9.com/5814140/which-poisonous-bacterial-protein-is-best-for-you/all
*  Category:Bacterial enzymes
category bacterial enzymes category bacterial enzymes bacterial enzymes are bacterial proteins whose main functions include catalytitic operations category bacterial proteins category enzymes...
https://en.wikipedia.org/wiki/Category:Bacterial_enzymes
*  List of strains of Escherichia coli
... escherichia coli is a well studied bacterium that was first identified by theodor escherich after whom it was later named strains innocuous escherichia coli strain nissle also known as mutaflor laboratory e coli k one of two laboratory strains innocuous clifton wild type w dh αe dam dcm strain escherichia coli b the other of the two lab strains from which all lab substrains originate escherichia coli bl de pathogenic enterotoxigenic e coli etec enteropathogenic e coli epec enteroinvasive e coli eiec enterohemorrhagic e coli ehec uropathogenic e coli upec verotoxin producing e coli e coli o h is an enterohemorrhagic strain also north american e coli outbreak e coli o h also e coli o h outbreak escherichia coli o escherichia coli o h escherichia coli k meningitis e coli o escherichia coli nc shigella shigella flexneri shigella dysenteriae shigella boydii shigella sonnei references category enterobacteria category escherichia coli category gram negative bacteria...
https://en.wikipedia.org/wiki/List_of_strains_of_Escherichia_coli
*  E Coli (Bacteria)
E Coli Bacteria. E Coli. Real Estate. Video. {"type":"article","show header text":false,"header":"ARTICLES ABOUT E COLI BACTERIA ","query":" des = \"E COLI BACTERIA \" ","search query":" subject:\"E COLI \\ BACTERIA\\ \" ","num search articles":"10","show summary":true,"show byline":true,"show pub date":true,"hide thumbnails":false,"show kicker":false,"show title":false,"show related topics":true,"show rad links":true,"show subtopics":true,"exclude topics":"E COLI BACTERIA ","more on header":"MORE ON E COLI BACTERIA AND:","alternate index subidx":"","show thumbnails":true}. MORE ON E COLI BACTERIA AND: Editorials, Hamburgers, Cattle, Factory Farming, Meat, Consumer Protection, Food Contamination and Poisoning, Consumer Reports, Pew Charitable Trusts, Beef, Food and Drug Administration, Agriculture Department, E Coli Bacteria, Salmonella Bacteria. MORE ON E COLI BACTERIA AND: Food Contamination and Poisoning, Salmonella Bacteria, E Coli Bacteria, United States, Law and Legislation, Agriculture Department. MORE...
http://topics.nytimes.com/top/reference/timestopics/subjects/e/e_coli_bacteria/index.html?query=Labeling and Labels&field=des&match=exact
*  Isolation, Cloning and Expression of Recombinant Human Renin in Escherichia Coli System - Universi
... ti Putra Malaysia Institutional Repository. Admin Login. Search. Faculty and Institute. Admin Login. Isolation, Cloning and Expression of Recombinant Human Renin in Escherichia Coli System. Ng, Chyan Leong 2002 Isolation, Cloning and Expression of Recombinant Human Renin in Escherichia Coli System. Masters thesis, Universiti Putra Malaysia. Therefore, recombinant protein technologies are used to produce the recombinant human renin protein. In this study, the full-length human renin coding gene REN was isolated from the human kidney cDNA library by using the polymerase chain reaction PCR technique. The PCR amplified REN gene was cloned into pCR-Blunt cloning vector. The REN gene was cloned into two different E. coli expression vectors, pRSETB and pGEX4T l, to express the recombinant protein. coli strains BL2 1-S1 and BL21 DE3 pLysS with the recombinant protein corresponding to the expected size -48 kDa. Both recombinant proteins have been confirmed with western blotting by using monoclonal anti-His antibod...
http://psasir.upm.edu.my/8486/
*  Recombinant Protein Production in Prokaryotic Systems | Wistar
Recombinant Protein Production in Prokaryotic Systems. Wistar. Postdoctoral Programs Predoctoral Programs High School Programs. Annual Biology Essay Contest Biomedical Research for High School Students Awards for Trainee Excellence Chemistry-Biology Interface Training Program Biomedical Technician Training Program Cancer Biology Graduate Program Support Wistar. Wistar Centers. Protein Expression Facility. Services Recombinant Protein Production in Prokaryotic Systems Our Science. Wistar Centers Research Areas Scientists Support and Infrastructure Centers Shared Resources Cores. Recombinant Plasmid DNA Engineering Recombinant Protein Production via BVES Recombinant Protein Production in Prokaryotic Systems Recombinant Protein Purification Retrovirus Production How to Obtain Services Helpful Information Instrumentation Policies and User Fees Contact Us Proteomics Facility Translational Research Management Facility Vector Core. Recombinant Protein Production in Prokaryotic Systems Recombinant Protein Production ...
http://wistar.org/our-science/shared-resources-cores/protein-expression-facility/services/recombinant-protein-produc-0
*  Niekonwencjonalne metody wykrywania Escherichia coli w wodzie do picia - Technologia Wody - Tom Nr 5
... 2012 - BazTech - Yadda. Szukaj. Przeglądaj. Pomoc. O bazie. . Narzędzia. Preferencje. Polski. English. Język Widoczny. Abstrakt 10 20 50 100. Liczba wyników. Artykuł - szczegóły. http://yadda.icm.edu.pl:80/baztech/element/bwmeta1.element.baztech-article-BPC2-0011-0006. Czasopismo Technologia Wody. Tytuł artykułu Niekonwencjonalne metody wykrywania Escherichia coli w wodzie do picia. Autorzy Trela, B. Papciak, D. Treść / Zawartość. Warianty tytułu EN Unconventional methods of detecting Escherichia coli in drinking water. Języki publikacji PL. Abstrakty PL W artykule przedstawiono sposoby wykrywania bakterii Escherichia coli w wodzie, oparte na konwencjonalnych i nowoczesnych metodach detekcji z zastosowaniem biosensor w. Zaprezentowano mechanizmy detekcji oraz techniki przetwarzania sygna u. Biosensory ze wzgl du na szybki pomiar s alternatyw dla konwencjo-nalnych metod opartych na izolowaniu i hodowaniu bakterii. Pozwalaj na szybkie reagowanie w przypadku stwierdzenia ska enia, chocia granica wykrywalno ...
http://yadda.icm.edu.pl/baztech/element/bwmeta1.element.baztech-article-BPC2-0011-0006
*  Common Drugs and Medications to Treat Appendicitis caused by Escherichia Coli Bacteria
... . Skip to content. Enter Search Keywords. Use the arrow keys to navigate suggestions. Symptoms. Doctors. Health Care Reform. Health A-Z. Common Conditions View All. ADD/ADHD. Allergies. Arthritis. Cancer. Cold, Flu Cough. Depression. Diabetes. Eye Health. Heart Disease. Heartburn/GERD. Pain Management. Sexual Conditions. Skin Problems. Sleep Disorders Featured Topics Identifying Bugs and Their Bites. Bothered by Yeast Infections. The Worst Shoes for Your Feet. WebMD Symptom Checker Health concern on your mind. See what your medical symptoms could mean, and learn about possible conditions. Get Started. Resources Second Opinion: Read expert perspectives on popular health topics. Communities: Connect with people like you, and get expert guidance on living a healthy life. Insurance Guide: Get ready for changes to your health care coverage. Physician Directory: Find a doctor in your area. WebMD Pain Coach Track your pain levels, triggers, and treatments. Set goals and get tips with our app for iPhone. Drugs S...
http://webmd.com/drugs/condition-2068-Appendicitis caused by Escherichia Coli Bacteria.aspx?diseaseid=2068&diseasename=Appendicitis caused by Escherichia Coli Bacteria&source=3&sortColumn=4&sortDirection=a
*  Intel Wants to Put a Supercomputer in Your Pock...
You are the content you publish Get Started for FREE Sign up with Facebook. Sign up with Twitter I don't have a Facebook or a Twitter account Need content for your business. Scooped by ComplexInsight onto Complex Insight - Understanding our world. ComplexInsight's insight: IF you are involved in developing or using professional mapping, survey and photogrammetric products - Dielmo have released their back office cloud platform that enables teams to upload, store, process and share LiDAR, Raster, Vector data derived from UAV, Helicopter, Fixed-wing and Mobile platforms. ComplexInsight's insight: As evidence builds that fruit bats may be a vector for the recent ebola outbreak in Western Africa - I was reminded of this paper in CDC's EID journal which found 5 out of 276 3.5% tested bats in Bangladesh had antibodies to Ebola. ComplexInsight's insight: Discussion of limits is key to creating new ideas - Igor Markov's paper is worth reading for exploring lmitations and engineering implications and to trigger off ne...
http://scoop.it/t/complex-insight/p/3179502120/2012/11/02/intel-wants-to-put-a-supercomputer-in-your-pocket
*  Jeff Hawkins Develops a Brainy Big Data Company...
You are the content you publish Get Started for FREE Sign up with Facebook. Sign up with Twitter I don't have a Facebook or a Twitter account Need content for your business. Scooped by ComplexInsight onto Complex Insight - Understanding our world. ComplexInsight's insight: IF you are involved in developing or using professional mapping, survey and photogrammetric products - Dielmo have released their back office cloud platform that enables teams to upload, store, process and share LiDAR, Raster, Vector data derived from UAV, Helicopter, Fixed-wing and Mobile platforms. ComplexInsight's insight: As evidence builds that fruit bats may be a vector for the recent ebola outbreak in Western Africa - I was reminded of this paper in CDC's EID journal which found 5 out of 276 3.5% tested bats in Bangladesh had antibodies to Ebola. ComplexInsight's insight: Discussion of limits is key to creating new ideas - Igor Markov's paper is worth reading for exploring lmitations and engineering implications and to trigger off ne...
http://scoop.it/t/complex-insight/p/3566001896/2012/12/02/jeff-hawkins-develops-a-brainy-big-data-company
*  Interview with Brian Mathews, Group CTO @ Autod...
You are the content you publish Get Started for FREE Sign up with Facebook. Sign up with Twitter I don't have a Facebook or a Twitter account Need content for your business. Scooped by ComplexInsight onto Complex Insight - Understanding our world. ComplexInsight's insight: IF you are involved in developing or using professional mapping, survey and photogrammetric products - Dielmo have released their back office cloud platform that enables teams to upload, store, process and share LiDAR, Raster, Vector data derived from UAV, Helicopter, Fixed-wing and Mobile platforms. ComplexInsight's insight: As evidence builds that fruit bats may be a vector for the recent ebola outbreak in Western Africa - I was reminded of this paper in CDC's EID journal which found 5 out of 276 3.5% tested bats in Bangladesh had antibodies to Ebola. ComplexInsight's insight: Discussion of limits is key to creating new ideas - Igor Markov's paper is worth reading for exploring lmitations and engineering implications and to trigger off ne...
http://scoop.it/t/complex-insight/p/3567407948/2012/12/03/interview-with-brian-mathews-group-cto-autodesk
*  Big Data Myths…BUSTED. | Innovation | Co...
You are the content you publish Get Started for FREE Sign up with Facebook. Sign up with Twitter I don't have a Facebook or a Twitter account Need content for your business. Scooped by ComplexInsight onto Complex Insight - Understanding our world. ComplexInsight's insight: Open science is critically important to future development and increasingly under attack from a wired varied of scources - sadly including the government - worth reading. ComplexInsight's insight: As evidence builds that fruit bats may be a vector for the recent ebola outbreak in Western Africa - I was reminded of this paper in CDC's EID journal which found 5 out of 276 3.5% tested bats in Bangladesh had antibodies to Ebola. ComplexInsight's insight: Discussion of limits is key to creating new ideas - Igor Markov's paper is worth reading for exploring lmitations and engineering implications and to trigger off new discussions and ideas. ComplexInsight's insight: Knowing which machine learning method to apply for a given problem takes time to...
http://scoop.it/t/complex-insight/p/2008326571/2012/06/21/big-data-myths-busted-innovation
*  Interactive Physical Simulation | Complex Insig...
You are the content you publish Get Started for FREE Sign up with Facebook. Sign up with Twitter I don't have a Facebook or a Twitter account Need content for your business. Scooped by ComplexInsight onto Complex Insight - Understanding our world. If interactive physics or comptuer graphics is your thing then you need to read tje collected Research from Computer Graphics Group at University of Copenhagen. ComplexInsight's insight: As evidence builds that fruit bats may be a vector for the recent ebola outbreak in Western Africa - I was reminded of this paper in CDC's EID journal which found 5 out of 276 3.5% tested bats in Bangladesh had antibodies to Ebola. ComplexInsight's insight: Discussion of limits is key to creating new ideas - Igor Markov's paper is worth reading for exploring lmitations and engineering implications and to trigger off new discussions and ideas. ComplexInsight's insight: Knowing which machine learning method to apply for a given problem takes time to learn - fortunately the fine folks ...
http://scoop.it/t/complex-insight/p/2807456924/2012/09/27/interactive-physical-simulation
*  E. Coli Growth Curves
Coli Growth Curves. E Coli Growth Curves Paul N Hengen pnh at ncifcrf.gov. Previous message: Is ligation reaction volume only 10 ul. Coli Growth Curves Messages sorted by:. Joe Boutell boutell at cf.ac.uk wrote:. coli to see how expression of a variety of plasmids affects them; what's the best way. Can you spin down the cells and weigh them, or is taking an OD more accurate and at what wavelength. The OD will not tell you if the plasmid is stable in E. coli. For studying that you'll have to plate dilutions on selective media. But how about if you're growing the E. coli up in selective liquid media. media, and if most of the bugs are chucking out a particular plasmid. Joe Boutell. If you grow a population of bacteria in selective liquid media, a certain proportion of the cells will not carry the plasmid and they will not be resistant to the antibiotic, yet grow in liquid. You can prove this if you want, but my feeling is that growing up a culture in liquid and determining density is different from determining ...
http://bio.net/bionet/mm/methods/1997-June/058458.html
*  .. E Coli Bacteria: A Few Interesting Facts .. Related Articles: .. 9 Responses to “E Coli Bacter
Home » Health Conditions » Viral Infections » Other Viruses E Coli Bacteria: A Few Interesting Facts. E Coli Can Be Helpful or Harmful Depending on the Strain Hand Washing is the Number One Prevention Method for E. Coli Bacteria Since you can’t always make sure that people do their part in avoiding spreading infections, you can be sure to gird up your immune system to combat anything that comes your way. Check out products like Echinacea/Goldenseal w/Org. Alcohol, Ester C 500mg and Sambucus Immune Support/AF to give your immune system the boost it needs. What are the Main Causes of Viral Infections. Differences between Viral and Bacterial Infections. All You Need to Know about Viral Infections. 9 Responses to “E Coli Bacteria: A Few Interesting Facts”. coli is a fecal form of bacteria, and is mainly transmitted in fecal form or growing in the large intestine. coli are not harful but instead helpful, and like sarah said, it would be quite difficult for E. coli to survive in your drinking water unless it was a ...
http://nutralegacy.com/blog/general-healthcare/e-coli-bacteria-a-few-interesting-facts/
*  Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12. -
... Lancaster EPrints. Lancaster EPrints. Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12. Copeland, Nikki and Bingham, Ryan and Georgiou, Theonie and Cooper, Peter and Kleanthous, Colin 2004 Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12. Bacteriophage exclusion is a suicide response to viral infection. In strains of Escherichia coli K-12 infected with T4 phage this process is mediated by the host-encoded Lit peptidase. Lit is activated by a unique sequence in the major head protein of the T4 phage the Gol sequence which then cleaves site-specifically the host translation factor EF-Tu, ultimately leading to cell death. Lit has very low sequence identity with other peptidases, with only a putative metallopeptidase motif, H 160 EXXH, giving an indication of its catalytic activity. The aim of the present study was to ascertain if Lit is a metallopeptidase, identify residues essential for Lit activity, and probe t...
http://eprints.lancs.ac.uk/52425/
*  Number of species of bacteria in mammalian la - bacteria - BNID 105655
... Home \ Search. Browse. Resources. Cell Biology by the Numbers. Login \ Submit. Popular BioNumbers. Recent BioNumbers. Key BioNumbers. Amazing BioNumbers. Find Terms. e.g., ribosome coli, p53 human, transcription, OD. BioNumber Details Page. Click search to return to the full list. ID 105655. Property Number of species of bacteria in mammalian large intestine. Organism bacteria. Range 400-500. Units unitless. Reference Peekhaus N, Conway T. What's for dinner?: Entner-Doudoroff metabolism in Escherichia coli. Reference PubMed ID 9657988. Primary Source Borrelio S P. Microbial flora of the gastro-intestinal tract. In: Hill M J, editor. Microbial metabolism in the digestive tract. Boca Raton, Fla: CRC Press, Inc. 1986. pp. 2–16. AND Finegold S M, Sutter V L, Mathisen G E. Normal indigenous intestinal microflora. In: Hentges D J, editor. Human intestinal microflora in health and disease. New York, N.Y: Academic Press, Inc. 1983. pp. 3–31.AND Hill M J. The normal gut bacterial flora. In: Hill M J, editor. Role...
http://bionumbers.hms.harvard.edu/bionumber.aspx?id=105655&ver=5
*  BL21(DE3) and trc promoter
bl de and trc promoter bl de and trc promoter chenha hzhen at freeuk com tue feb est previous message bl de and trc promoter next message bl de and trc promoter messages sorted by petri kursula wrote confused by all these e coli genotypes and strains i would like to know if anyone has used bl de cells to produce recombinant proteins from a vector with the trc promoter such as ptrc a if yes is this expression tightly regulatable by iptg or do you get leaky expression this is likely to be leaky i haven t used ptrc a for a long time but i think it has a laciq which should help with the regulation in general as long as the expression is reasonably well controlled it should be ok if your protein is not toxic to the cell you only need to worry about how tight the regulation is if your protein is toxic in which case ptrc a is a bad choice and you will have to consider using other vectors btw the de bit of your bl de is unncessary for ptrc a if you are confused by the strains all you need to know is that there are cl...
http://bio.net/bionet/mm/methods/2000-February/080939.html
*  .. Over 400 articles on the E. coli bacteria available online on SpringerLink .. Post navigation
coli bacteria available online on SpringerLink. Springer Science+Business Media is offering all journal articles and book chapters which deal with the E. coli bacteria free of charge on its online information platform www.springerlink.com. The articles can be found by using the search terms “Enterohaemorrhagic and Escherichia and coli” or by using the link www.springer.com/ehec. A total of over 400 scientific articles are available to print out or download from now until 1 September 2011. coli strain of bacterium has the potential to cause severe diarrhea, followed by serious organ system damage. During the past few weeks, a significant increase in the number of patients with the disease has been reported in Europe, especially in Germany. The European Center for Disease Prevention and Control says transmission of the strain of bacterium, commonly found in cattle, usually occurs through contaminated food or water and contact with animals. Eric Merkel-Sobotta, Executive Vice President Corporate Communicatio...
http://libsys.uah.edu/LibraryBlog/wordpress/?p=482
*  Site - glnVSite glnV
CGSC Coli Genetic Stock Center. E coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center. About the CGSC CGSC Home:. The Database Acknowledgment of Support Searching the CGSC Database Help With Query Forms Help with Genetic Nomenclature Strain Query Mutation Query Gene/Site Query Gene Product Query Reference Query Query Help How to Request Strains or Information Contact Information Charges How We Ship Strains Strains Not Found. Other CGSC Information FAQ on Procedures Current Working Map 1998 MMBR Map 1998 MMBR Gene List Map Diagrams PDFs Other Links E. coli Links Other Stock Collections Other Bio Links Yale Web. Site - glnV Site glnV Site glnV Name: glnV Type: Gene. Mnemonic: Glutamine Left End Point: 14.99 Right End Point: 15.00 Products: glutamine tRNA2. Direction: < Properties: Property Comment amber suppressor NULL. tRNA gene glutamine tRNA2. duplicate gene tandem;. glnV22 AS. glnV33 AS. glnV41 AS. glnV42 AS. glnV89 AS. glnV93 AS. External Database Links: Host Site Page Links EcoGene.org EG30...
http://cgsc.biology.yale.edu/Site.php?ID=695
*  Mutation - lpxL10(ts)::mini-Tn10Mutation lpxL10(ts)::mini-Tn10
cgsc coli genetic stock center e coli genetic resources at yale cgsc the coli genetic stock center pay an invoice about the cgsc cgsc home scope of the collection the database acknowledgment of support searching the cgsc database help with query forms help with genetic nomenclature strain query mutation query gene site query gene product query reference query query help how to request strains or information contact information charges how we ship strains strains not found other cgsc information faq on procedures current working map mmbr map mmbr gene list map diagrams pdfs other links e coli links other stock collections other bio links yale web funded by a grant from the national science foundation mutation lpxl ts mini tn mutation lpxl ts mini tn mutation lpxl ts mini tn name lpxl ts mini tn type tn mutation of gene lpxl approx map location properties property symbol temperature sensitive ts tetracycline resistant comments grows in rich media below c but not at higher temperatures references karow m s raina...
http://cgsc.biology.yale.edu/Mutation.php?ID=65138
*  OCA Atlas for 1SC4
... 1SC4 Hydrolase. date Feb 11, 2004. . title Crystal Structure Of The Human Caspase-1 C285a Mutant After Malonate authors M.J.Romanowski, J.M.Scheer, T.O'Brien, R.S.Mcdowell compound source. Molecule : Interleukin-1 Beta Convertase Chain : A Fragment : Interleukin-1 Beta Convertase P20 Synonym : Il-1bc, Il-1 Beta Converting Enzyme, Ice, Interleu Converting Enzyme, P45, Caspase-1, Casp-1; Ec : 3.4.22.36 Engineered : Yes Mutation : Yes Organism scientific : Homo Sapiens Organism common : Human Organism taxid : 9606 Gene : Casp1, Il1bc, Il1bce Expression system : Escherichia Coli Expression system taxid : 562 Expression system strain : Bl21 De3 Codon+ Expression system vector type : Plasmid Expression system plasmid : Prset. Molecule : Interleukin-1 Beta Convertase Chain : B Fragment : Interleukin-1 Beta Convertase P10 Synonym : Il-1bc, Il-1 Beta Converting Enzyme, Ice, Interleu Converting Enzyme, P45, Caspase-1, Casp-1; Ec : 3.4.22.36 Engineered : Yes Organism scientific : Homo Sapiens Organism common : Huma...
http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SC4
*  OCA Atlas for 3L9J
... 3L9J Immune System. date Jan 05, 2010. . title Selection Of A Novel Highly Specific Tnfalpha Antagonist: In The Crystal Structure Of The Antagonist-Tnfalpha Complex authors P.Byla, M.H.Andersen, H.C.Thogersen, H.H.Gad, R.Hartmann compound source. Molecule : Tnfalpha Chain : C Engineered : Yes Organism scientific : Homo Sapiens Organism common : Human Organism taxid : 9606 Expression system : Escherichia Coli Expression system taxid : 562 Expression system vector type : Plasmid Expression system plasmid : Pt7ciih6. Molecule : Tumor Necrosis Factor, Soluble Form Chain : T Fragment : Unp Residues 85-233 Synonym : Tnf-Alpha, Tumor Necrosis Factor Ligand Superfamil 2, Tnf-A, Cachectin; Engineered : Yes Organism scientific : Homo Sapiens Organism common : Human Organism taxid : 9606 Expression system : Escherichia Coli Expression system taxid : 562 Expression system vector type : Plasmid Expression system plasmid : Pt7ciih6. symmetry. Space Group : P 63 2 2 R factor 0.173. R Free 0.217. crystal cell. length a ...
http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9J
*  .. E. coli found in Hazy Hill water supply
E coli found in Hazy Hill water supply. March 31, 2012 Williamson County Politics. TRAVIS COUNTY KXAN – Residents of the Hazy Hill community, a small residential area off State Highway 71 near Spicewood, learned E. coli was found in their water system two days after the test came back positive. Gulf Utility Service Inc., the Houston-based company responsible for supplying the water, sent a note to residents dated March 23. Most residents received the letter the following day and were using contaminated water without knowing it. E coli or Escherichia coli bacteria, according to the Mayo Clinic, normally live in the intestines of healthy people and animals. Most varieties of E. coli are harmless or cause relatively brief diarrhea. But a few particularly nasty strains, such as E. Healthy adults usually recover from infection with E. coli O157:H7 within a week, but young children and older adults can develop a life-threatening form of kidney failure called hemolytic uremic syndrome. The letter told residents a w...
http://txwclp.org/2012/03/e-coli-found-in-hazy-hill-water-supply/
*  dut- ung- E. coli strain request
dut ung e coli strain request dut ung e coli strain request the wagner lab ewlab at uci edu thu feb est previous message dut ung e coli strain request next message dut ung e coli strain request messages sorted by ottok at u washington edu kevin g otto wrote we are looking for a dut ung e coli strain named cj or any other transformable dut ung strain thanks kevin otto kevin i know this strain is available from biorad as part of their mutagene kit if you re too cheap to buy it ask some of your neighbors i m sure that someone has to have it as it is a fairly common strain matthew petroski mdpetros at uci edu molecular biology and biochemistry university of california irvine previous message dut ung e coli strain request next message dut ung e coli strain request messages sorted by more information about the methods mailing list...
http://bio.net/bionet/mm/methods/1996-February/041156.html
*  BISC209: Lab4 - OpenWetWare
room temperature BR. room temperature BR. Today you will insert your bacterial 16s rRNA gene fragments into a patented cloning vector pCR-BluntII TOPO® and then transform that vector into a special genetically engineered strain of Escherichia coli bacteria that will express a vector gene for kanamycin resistance, allowing us to select for transformants on media containing kanamycin. 4 Add 1 μL of pCR®II-Blunt-TOPO® cloning vector plasmid. Transforming One Shot® Competent Cells Introduction : Once you have performed the TOPO® Cloning reaction, you will transform your pCR®-Blunt II-TOPO® construct into TOPO10 competent E. Preparing for Transformation For each transformation, you will need one vial of competent cells and two selective plates. When medium size, ISOLATED colonies, have appeared, refrigerate your transformation plates until LAB 5. Make sure that these potentially pure cultures have colonies that look like the original source colony and that all of the colonies look the same they can be of different...
http://openwetware.org/index.php?title=BISC209:_Lab4&diff=424983&oldid=393895
*  SHuffle® Express Competent E. coli | NEB
SHuffle® Express Competent E. coli. NEB. My NEB. My NEB. SHuffle® Express Competent E. coli Products,. coli Expression Strains. E.coli Protein Expression,. FAQs Tech Tips. Other Tools Resources. Related Products. coli B cells engineered to form proteins containing disulfide bonds in the cytoplasm. Highlights Transformation efficiency: 1 x 10 7 cfu/µg pUC19 DNA Engineered E. coli cells. Is there anything I can do to increase protein yield when using SHuffle strains. Can I conduct blue/white screening using alpha complementation of lacZ in SHuffle strains. Datacards The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: Datasheet-Lot. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document. Individual lot data can be found on the Product Summary Sheet...
https://neb.com/products/c3028-shuffle-express-competent-e-coli
*  .. Return to Strain Description
CGSC Strain#: 7145 Strain Designation: KJ173      Source of Strain: J.R. Beckwith Sex: F- Chromosomal Markers:. araA-leu 7697 , B/r , codB-lacI 3 , phoR82 , zaj-2085::Tn10 , secD29 Cs , galK16 , galE15 GalS , - , e14- , relA1 , rpsL150 strR , spoT1 , mcrB1 Strain Comments:. araA-leu 7697 -- Shown by Durfee et al. to be a deletion of the first 295 codons of hepA to the first 82 codons of fruR. araA-leu 7697 -- Gerdes et al. show this deletion to cover from 63.4 kb to 89 kb, from polB through fruR. B/r -- The thr-leu region was transduced from an E. coli B/r strain SB3118 in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B. codB-lacI 3 -- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001. codB-lacI 3 -- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE. codB-lacI 3 -- Shown by Ferrandez et al. 1997 to extend to mhpD secD29 Cs -- : The secD lo...
http://cgsc.biology.yale.edu/StrainRpt.php?ID=38551
*  .. Return to Strain Description
CGSC Strain#: 7729 Strain Designation: ALS225      Source of Strain: E. Altman Sex: F' Episome/Plasmid: F128-xxx Plasmid Markers/Mutations: Chromosomal Markers:. araA-leu 7697, B/r, codB-lacI 3, galK16, galE15 GalS, -, e14-, mcrA0, relA1, rpsL150 strR, spoT1, mcrB9999, hsdR2 Strain Comments:. araA-leu 7697 -- Shown by Durfee et al. to be a deletion of the first 295 codons of hepA to the first 82 codons of fruR. araA-leu 7697 -- Gerdes et al. show this deletion to cover from 63.4 kb to 89 kb, from polB through fruR. B/r -- The thr-leu region was transduced from an E. coli B/r strain SB3118 in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B. codB-lacI 3 -- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001. codB-lacI 3 -- Shown by Ferrandez et al. 1997 to extend to mhpD. codB-lacI 3 -- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE g...
http://cgsc.biology.yale.edu/StrainRpt.php?ID=76211
*  Protein toxity in E.coli
protein toxity in e coli protein toxity in e coli al r plummer aplummer at brain uccs edu fri jun est previous message inappropriate messages next message high copy trp library messages sorted by our lab is trying to overexpress and isolate a rather large yeast s cerevisiae protein in e coli the gene is ca kb and encodes a protein of a a we transformed dh alpha with a plasmid containing the gene but are unable to see it also when the expression is induced iptg the e coli become sick grow slower does anyone have any experience expressing large or toxic proteins in e coli any help would be greatly appreciated al plummer at em uni frankfurt de previous message inappropriate messages next message high copy trp library messages sorted by more information about the yeast mailing list...
http://bio.net/bionet/mm/yeast/1997-June/007099.html
*  E.Coli Westerns
e coli westerns e coli westerns dan szymanski dan szymanski at qms life uiuc edu wed nov est previous message gel database next message e coli westerns messages sorted by greetings netters a couple of us have been trying to detect recombinant e coli proteins with rabbit polyclonal ab backround is horrendous does anyone have an explanation or suggestions to reduce backround thanks dan previous message gel database next message e coli westerns messages sorted by more information about the proteins mailing list...
http://bio.net/bionet/mm/proteins/1994-November/001915.html
*  BMW M3 Forum (E90 E92) - View Single Post - New E93 goodness
bmw m forum e e view single post new e goodness thread new e goodness view single post pm sor lieutenant rep posts drives e m mw fr join date jun location ut itrader no since they re mineral white they catch the light differently at different angles i imagine you re referring to the fourth photo see the first photo quote sor view public profile find more posts by sor...
http://m3post.com/forums/showpost.php?p=8340400&postcount=25
*  Need help:insoluble to soluble
need help insoluble to soluble need help insoluble to soluble jared quentin lemaster lemaster at osu edu mon oct est previous message announce vmd beta next message need help insoluble to soluble messages sorted by hi i expressed foreign protein in e coli to the level of about total protein after iptg induction but it is insoluble does anyone have any experiences that could make it soluble without loss of function or expressed as a soluble protein in e coli pls send your suggestion to wang at osu edu thanks a lot previous message announce vmd beta next message need help insoluble to soluble messages sorted by more information about the proteins mailing list...
http://bio.net/bionet/mm/proteins/1999-October/007531.html
*  Panasonic 14mm F2.5 review data at various sites.: Micro Four Thirds Talk Forum: Digital Photography
... Review. Reviews. Sample Images. Videos. Lenses. Forums. Forum index. Micro Four Thirds Talk Change forum Panasonic 14mm F2.5 review data at various sites. Shop cameras lenses ▾. Forum Parent First Previous Next Next unread. jericho77. Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Reply to thread Reply with quote. Forum Parent First Previous Next Next unread. Posted by When Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 3. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Aug 5, 2013. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. jericho77. Aug 5, 2013. Re: Panasonic 14mm F2.5 review data at various sites. Aug 5, 2013 1. Re: Panasonic 14mm F2.5 review data at various sites. Alumna Gorp. Aug 5, 2013. ...
http://dpreview.com/forums/post/51930238
*  The genetic basis of a plant-insect coevolutionary key innovation. Proc Natl Acad Sci USA (PDF Downl
Proc Natl Acad Sci USA PDF Download Available. Proc Natl Acad Sci USA. © 2007 by The National Academy of Sciences of the USA www.pnas.org?cgi?doi?10.1073?pnas.0706229104PNAS. fossil calibrated, Bayesian relaxed molecular clock estimation of the Pierinae–Coliadinae divergence. Fossil and molecular data agree that the Brassicales appeared by 90 to 85 Mya, which is much earlier than the parallel evolution of glucosinolates in the Euphorbiaceae 58 Mya 28. In this article, we directly address the timing of the appearance of the glucosinolate-feeding Pierinae, using several independent molecular datasets and various calibration methods to generate a robust estimate of when Pieridae evolved relative to their Brassicales host plants. Pieridae-specific temporal reconstruction used a Bayesian relaxed molecular clock method on EF-1. Current and expected diversity, and divergence estimates for the Coliadinae and Pierinae subfamilies of Pieridae. However, there are significantly more species of Pierinae than expected, eve...
http://researchgate.net/publication/5771953_The_genetic_basis_of_a_plant-insect_coevolutionary_key_innovation._Proc_Natl_Acad_Sci_USA
*  Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protecti
... ve biofilms -- ScienceDaily. Your source for the latest research news. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms. Date: April 26, 2012 Source: The Scripps Research Institute Summary: Scientists have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Scientists at The Scripps Research Institute have unraveled a complex chemical pathway that enables bacteria to form clusters called biofilms. Past research had also revealed that nitric oxide is involved in influencing bacterial biofilm formation. Nitric Oxide Modulates Bacterial Biofilm Formation through a Multicomponent Cyclic-di-GMP Signaling Network. The Scripps Research Institute. "Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biofilms." ScienceDaily. The Scripps Research Institute. Mystery of bacterial growth and resistance solved: Findings shed light on how bacteria form protective biof...
http://sciencedaily.com/releases/2012/04/120426135128.htm
*  Mankin Lab - Publications
Browse by year: 2014    2013    2012    2011    2010    2009    2008    2007    2006    2005    2004    2003    2002    2001    2000    1999    1998    1997    1996    1995   . Proc Natl Acad Sci USA 111, 9804-9809. 2014 The general mode of translation inhibition by macrolide antibiotics Proc Natl Acad Sci USA 111, 15958–15963. Top. Top. Top. Proc Natl Acad Sci USA 108, 5931-5932. Proc Natl Acad Sci USA 108, 10496-10501. Vázquez-Laslop, N., Ramu, H., Mankin, A. 2011 Nascent peptide in the ribosome exit tunnel affects functional properties of the A-Site of the peptidyl transferase center Mol Cell 41, 321-330. Top. Proc Natl Acad Sci USA 107, 1983-1988. A., Xiong, L., Mankin, A. H D 2010 Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107, 17152-...
http://mankinlab.cpb.uic.edu/cgi-bin/publication.cgi
*  GO:1900911 regulation of olefin biosynthetic process
... Services. Research. Training. Industry. About us. QuickGO A fast browser for Gene Ontology terms and annotations. EBI Databases QuickGO GO:1900911 regulation of olefin biosynthetic process. Search for terms by keyword or ID: apoptosis GO:0006915 Search for proteins by name or accession: tropomyosin P06727. Web Services. Dataset. Term Basket. Term Information ID GO:1900911. Name regulation of olefin biosynthetic process. Ontology Biological Process. Definition Any process that modulates the frequency, rate or extent of olefin biosynthetic process. GONUTS GO:1900911 Wiki Page. Synonyms. Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope. Click on the icon for more details. Type Synonym. exact regulation of olefin synthesis. exact regulation of olefin biosynthesis. exact regulation of olefin formation. exact regulation of olefin anabolism. Ancestor Chart This chart is interactive; ...
http://ebi.ac.uk/QuickGO/GTerm?id=GO:1900911
*  Albums by Smokey Robinson : Rhapsody
... JavaScript is disabled in your browser settings. rhapsody.com requires JavaScript. Cancel. Free trial. x Music. Apps Devices. Pricing. Sign up. Company Info. Careers. Press Media. Partners. Account. Customer Support. Redeem Coupon. Buy a Gift. 2015 Rhapsody International Inc. ×. Rhapsody App for. Rhapsody International, Inc. Get app. Have the app. Music Apps Devices Pricing Listen Now Try Rhapsody. Sign In. Home. / Music. / Soul/R B. / Motown. / Smokey Robinson. / Facebook. Twitter. Albums by Smokey Robinson. Main Releases. Play. Smokey & Friends. Smokey Robinson. Play. Be Near Me. Smokey Robinson. Play. The Motown Years Live. Smokey Robinson. Play. Ev'ry Man Should Know. Smokey Robinson. Play. The Solo Anthology. Smokey Robinson. Play. The Stripped Mixes. Smokey Robinson. Play. Time Flies When You're Having Fun. Smokey Robinson. Play. Love Songs. Smokey Robinson. Play. Timeless Love. Smokey Robinson. Play. The Definitive Collection. Smokey Robinson. Play. 20th Century Masters: The Millennium Collection....
http://rhapsody.com/artist/smokey-robinson/albums
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/451.aspx?email=1
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/fuchs_appointed_as_general_counsel_16.aspx
*  Robinson News | News | News Releases | Robinson Memorial Hospital
-Any- Foundation News Robinson News. Foundation Awards Scholarships Benefitting Employees September 25, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the awarding of 9 scholarships to employees of Robinson Memorial Hospital and Rob... Robinson wins NorthCoast 99 Award 12 years in a row September 4, 2014 Robinson Memorial Hospital again has been named as one of the best 99 places to work in Northeast Ohio by the Employers Resource Council. Osteoporosis event to celebrate healthy aging month September 4, 2014 During Robinson Memorial Hospital’s celebration of healthy aging month, there will be a program on Osteoporosis on Wednesday, September 10 from 6 ... Foundation Awards Student Scholarships July 10, 2014 The Robinson Memorial Hospital Foundation is pleased to announce the award of seven scholarships to children of Robinson Memorial Hospital and Robi... Robinson Wound Care Center Joins Healogics, Inc. to Raise Awareness of Chronic Wounds May 29, 2014 The Robinson Wound Care Center at ...
http://robinsonmemorial.org/main/news-releases/robinson_memorial_presents_poster_at_the_apic_annu_494.aspx?email=1
*  Robinson Health Center at Aurora | Robinson Memorial Hospital
robinson health center at aurora robinson memorial hospital about us foundation volunteer speaker s bureau press room contact us patients visitors medical services nursing find a doctor locations careers patient portal home locations robinson health center at aurora robinson health center at aurora walden place aurora ohio the robinson health center at aurora offers expert physicians personalized care and the latest in medical technology in one convenient location services and physician practices aurora primary care diagnostic x ray and laboratory services northeast ohio ob gyn robinson rehab center and sport clinic directions view larger map your name your email recipient email your comments word verification visitors patient portal key phone numbers visiting hours wheelchair assistance wi fi at robinson memorial hospital locations robinson memorial hospital robinson medical arts building robinson professional center robinson health center at aurora robinson health center at brimfield robinson health center ...
http://robinsonmemorial.org/Main/Aurora.aspx?email=1
*  Home | Robinson Memorial Hospital
Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Classes Events. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 2:00 PM - 4:00 PM. Holiday Journeys - Grief Support. Mon, Oct 5, 2015. 6:30 PM - 8:30 PM. Ostomy Support Group. Mon, Oct 5, 2015. 7:00 PM - 9:00 PM. Diabetes Support Group. Tue, Oct 6, 2015. 5:00 PM - 6:00 PM. Tue, Oct 6, 2015. 5:00 PM - 6:30 PM. Robinson In The News Robinson Memorial Hospital is now UH Portage Medical Center. Robinson Health System becomes part of University Hospitals on June 1. Robinson Memorial hosts 21st Annual Nursing Research Day. Robinson Memorial Hospital Foundation Hosts 2015 Robinson Society and Heritage Society Distinguished Giving Recognition. Doctors Receiving Top Scores in Patient Satisfaction Congratulations to the following doctors who rank in the 90th percentile on the Press Ganey Patient Satisfaction Survey. Jessica ...
http://robinsonmemorial.org/Main/Home.aspx
*  Rehabilitation | Robinson Memorial Hospital
Rehabilitation. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Locations. Patient Portal. Medical Services. Rehabilitation. Rehabilitation. The Robinson Rehab Center and Sport Clinic works to rehabilitate each patient to his or her maximum potential and works to provide the patient with the necessary skills to maintain his or her progress. Ongoing communication between the patient, therapist and referring physician is the foundation of the center's rehabilitation programs. View Aquatic Classes. Team of Professionals Each therapist strives to stay on top of the latest technology and treatments and passes this knowledge along to patients. All Robinson Rehab Center and Sport Clinic professionals are licensed or certified by the State of Ohio. The center provides:. Aquatic aerobics, aquatic exercise and water arthritis classes in Ravenna. Locations: Robinson Medical Arts Building. 6847 North Chestnut Street Ravenna, Ohio 44266...
http://robinsonmemorial.org/main/rehabilitation.aspx
*  Shamim Patel - UK address and phone number - 192.com
... People. All People Businesses Places Electoral Roll Directors. Make sure the biggest decision you'll make is also the best. People. AZ-People. Surname - Patel. Your results for Shamim Patel. Filter Search. Search by name and/or location using the search form at the top of the page. Get access to likely ages Find old friends and family Double check names of spouses and children Trace your family tree Find out who your neighbours are Verify people are who they say they are. Make sure the biggest decision you'll make is also the best. Find out more. previous. 110 Results for Shamim Patel. Looking for Shamim Patel. Below are the results from the UK Electoral Roll and Company Director data. You can narrow your search by adding a location above if you wish. Name Address Other Occupants Electoral Roll Director Info Length of Occupancy Neighbours Property Price. 1 Shamim Patel. Age Guide: 40-44. Reading, Berkshire, RG2 Full Address. View. 2 Shamim R Patel. Age Guide: 30-34. Leicester, Leicestershire, LE5 Full Ad...
http://192.com/atoz/people/patel/shamim/
*  Phillip Phillips News, Pictures, and Videos | TMZ.com
Home Celebs Phillip Phillips Phillip Phillips. Idol Managers Claim John Mayer's ex-manager plotted to jack "American Idol" champ Phillip Phillips out from under his contract with the show -- according to a new lawsuit, but the bigger news is Phil is a READ MORE > - 9/21/2015 2:51 PM PDT. Phillip Phillips I Want to be Gone, Gone, Gone From My Idol Contract Phillip Phillips says "American Idol" producers are ruining his career ... American Idol Winner Phillip Phillips Mother Popped for DUI Phillip Phillips' mother Sheryl Phillips -- who appeared on several episodes of "American Idol" during Phillip's championship run last season -- was arrested for DUI earlier today, TMZ has READ MORE > - 964 days ago. Phillip Phillips I LOVE MY FAMILY But ... READ MORE > - 1064 days ago. Phillip Phillips Family Idol Champ s Forcing Us To Sell Pawn Shop Phillip Phillips' family says the singer left them high and dry after winning "American Idol" -- refusing to help them out financially -- and now, they've been forced to sell th...
http://tmz.com/person/phillip-phillips/?adid=cr_featured_saga_13
*  Shadowing | Robinson Memorial Hospital
Shadowing. Robinson Memorial Hospital. ABOUT US FOUNDATION VOLUNTEER SPEAKER'S BUREAU PRESS ROOM CONTACT US. Patients Visitors. Medical Services. Nursing. Find a Doctor. Locations. Careers. Patient Portal. Home. Volunteer. Shadowing. Shadowing. Robinson Memorial Hospital offers several types of opportunities for short-term observational learning experiences for high school, college and adult students pursuing healthcare careers. The process to apply for a shadow experience depends on the age of the applicant. Note: Robinson Memorial Hospital has a mandatory flu vaccination policy for all employees, volunteers, contracted workers and shadow students. Students wanting to shadow at Robinson Memorial must provide proof of receiving a flu vaccine during current flu season - either by a receipt showing the student's name and detail of service from the provider, an insurance benefits explanation or detailed note from a physician. Students can bring their vaccine documentation the morning of their assigned shadow exp...
http://robinsonmemorial.org/Main/Shadowing.aspx
*  [Haskell-cafe] System.Random.Shuffle fix
System.Random.Shuffle fix. System.Random.Shuffle fix friggin friggin frigginfriggins at gmail.com. Previous message: A small display problem using Helium Next message: System.Random.Shuffle fix Messages sorted by:. I was looking for a shuffling algorithm to shuffle mp3-playlists so was very happy to see System.Random.Shuffle: http://hackage.haskell.org/cgi-bin/hackage-scripts/package/random-shuffle-0.0.2 However I get errors,non-exhaustive patterns in function shufleTree or extractTree depending how I call it. Errors are at the bottom. I fixed it but I don't have the math skills to see if I perhaps broke it statistically ... Here is my fix, someone don't remember who, helped me a little : http://hpaste.org/fastcgi/hpaste.fcgi/view?id=2789#a2789 the shuffle at the end is with the fix. *Freet S.shuffle Loading package syb ... linking ... Loading package base-3.0.3.0 ... linking ... Loading package old-locale-1.0.0.1 ... linking ... Loading package old-time-1.0.0.1 ... linking ... Loading package random-1.0.0.1 ...
https://mail.haskell.org/pipermail/haskell-cafe/2009-March/058399.html
*  OriGene - LRRC2 (NM 024512) cDNA Clone
OriGene - LRRC2 NM 024512 cDNA Clone. cDNA Clones TrueORF cDNA Clones H/M/R Viral ORF Clones Destination Vector TrueClone Human TrueClone Mouse Organelle Marker Plasmids MicroRNA Tools Mutant and Variant Clones Plasmid Purification Kits Transfection Reagents Gene Synthesis Service. Home TrueClone LRRC2 Clone. LRRC2 NM 024512 Human cDNA Clone. SC310473 LRRC2 untagged -Human leucine rich repeat containing 2 LRRC2, NM 024512.2, 10ug $680 3 weeks. Reference Data RefSeq: NM 024512.2, NP 078788. Synonyms : leucine-rich repeat-containing 2; leucine rich repeat containing 2. More TrueClone Citations >>. LRRC2 Mouse Clones SKU Description Amount Price Shipping. MC211501 Lrrc2 untagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $380 3 weeks. MR216368 Lrrc2 Myc-DDK-tagged - Mouse leucine rich repeat containing 2 Lrrc2 10 ug $380 Next day. MG216368 Lrrc2 GFP-tagged - Mouse leucine rich repeat containing 2 Lrrc2, 10ug 10 ug $420 3 weeks. MR216368L1 Lenti ORF clone of Lrrc2 Myc-DDK-tagged - Mouse leucine ri...
http://origene.com/cdna/trueclone/accession/NM_024512/SC310473.aspx
*  JUST LIKE A WOMAN LYRICS - BOB DYLAN
just like a woman lyrics bob dylan sing lyrics bob dylan biography bob dylan albums bob dylan lyrics bob dylan just like a woman lyrics last updated pm sponsored links nobody feels any pain tonight as i stand inside the rain ev rybody knows that baby s got new clothes but lately i see her ribbons and her bows have fallen from her curls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl queen mary she s my friend yes i believe i ll go see her again nobody has to guess that baby can t be blessed till she finally sees that she s like all the rest with her fog her amphetamine and her pearls she takes just like a woman yes she does she makes love just like a woman yes she does and she aches just like a woman but she breaks just like a little girl it s was raining from the first and i was dying there of thirst so i came in here and your long time curse hurts but what s worse is this pain in here i can t stay i...
http://sing365.com/music/Lyric.nsf/Just-Like-A-Woman-lyrics-Bob-Dylan/659E935998737E48482569690029ABC7
*  Gary Phillips eBooks | epub and pdf downloads | eBookMall
Gary Phillips eBooks. epub and pdf downloads. eBookMall. Search. Sponsored link: What is YOUR Secret Talent. - Take a Free Personality test. Gary Phillips eBooks Epub and PDF format. Gary Phillips eBooks. eBooks found: 15 Occupied Earth: Stories of Aliens, Resistance and Survival at all Costs. Richard Brewer. Gary Phillips Polis Books, October 2015. ISBN: 9781940610528 Format: ePub. List Price: $ 9.99 Our price: $ 7.99. Violent Spring. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087968 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Perdition, U.S.A. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087975 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Bad Night Is Falling. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087982 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. Only the Wicked. Gary Phillips mysteriouspress.com, June 2014. ISBN: 9781784087951 Format: ePub. List Price: $ 13.11 Our price: $ 9.99. The Extractors. Gary Phillips Stark Raving Group, M...
http://ebookmall.com/author/gary-phillips
*  "Beyond Stroke" Support Group | Events | Calendar of Events | Robinson Memorial Hospital
beyond stroke support group events calendar of events robinson memorial hospital about us foundation volunteer speaker s bureau press room contact us patients visitors medical services nursing find a doctor locations careers patient portal beyond stroke support group calendar view back to previous page support groups tuesday october pm pm description stroke survivors and caregivers will benefit from information presented by a variety of speakers as well as from sharing and support of their peers for information call jan bahle at location robinson medical arts building rmab n chestnut st ravenna ohio contact healtheducation rmh org phone visitors patient portal key phone numbers visiting hours wheelchair assistance wi fi at robinson memorial hospital locations robinson memorial hospital robinson medical arts building robinson professional center robinson health center at aurora robinson hea...
http://robinsonmemorial.org/Main/Calendar/659.aspx
*  Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine
... Home About Stories. Featured. BOR Featured. User Stories. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine. Deposition of Bobby Phillips States That Erwin Cain Sold Cocaine 3. By Burnt Orange Report. on October 19, 2010. Bobby Phillips, Jr. Phillips states that he met Erwin Cain in October of 1977, when he was engaged to Erwin's sister, Gloria Cain. Attorney : Did you ever see Mr. Erwin Cain sell drugs. Phillips : Yeah, I've seen him deliver to his brother-in-law, to his brother, and to other people that came by the house when he was there. Attorney : Do you know where Thomas Cain, Jr. Attorney: which brother would that be. Phillips : Erwin. Attorney: Just to clarify, and let me repeat it's your statement that you have seen, in the past, a man you can identify as Erwin Cain use marijuana. Phillips : Yes. Attorney: Have you, and is it your statement also that you have seen him sell marijuana to someone. Phillips : Yeah. Phillips : It was usually just we used to go over to his h...
http://burntorangereport.com/diary/10880/deposition-of-bobby-phillips-states-that-erwin-cain-sold-cocaine
*  Anthony Robinson | dafont.com
Anthony Robinson. dafont.com. English. Fran ais. Espa ol. Deutsch. Italiano. Portugu s Login. Register. Themes New fonts Authors Top Forum FAQ Submit a font Tools. 4 matching requests on the forum Anthony Robinson 1. 2 3. Anthony Robinson. View profile Send a private message http://www.redbubble.com/people/anfa/portfolio 58 fonts - 4,331,510 downloads 2,129 yesterday. Preview. Fonts 10 20 50 100. Show variants. Size Tiny Small Medium Large Sort by Name Popularity Newer first. More options. Only as. Public domain / GPL / OFL. 100% Free. Free for personal use. Donationware. Shareware. Demo. Unknown Only fonts with. Accents. Euro. Newcastle by Anthony Robinson. in Fancy. Decorative 15,200 downloads 4 yesterday. 100% Free - 2 font files Download Donate to author. Static Buzz by Anthony Robinson. in Fancy. Distorted 104,264 downloads 4 yesterday. 100% Free Download Donate to author. Lasso Of Truth by Anthony Robinson. in Gothic. Modern 5,855 downloads 4 yesterday. 100% Free Download Donate to author. Punched by An...
http://dafont.com/anthony-robinson.d2796?page=3
*  Anthony Robinson | dafont.com
Anthony Robinson. dafont.com. English. Fran ais. Espa ol. Deutsch. Italiano. Portugu s Login. Register. Themes New fonts Authors Top Forum FAQ Submit a font Tools. 4 matching requests on the forum Anthony Robinson 1. 2 3. Anthony Robinson. View profile Send a private message http://www.redbubble.com/people/anfa/portfolio 58 fonts - 4,333,980 downloads 2,470 yesterday. Preview. Fonts 10 20 50 100. Show variants. Size Tiny Small Medium Large Sort by Name Popularity Newer first Reinitialize More options. Only as. Public domain / GPL / OFL. 100% Free. Free for personal use. Donationware. Shareware. Demo. Unknown Only fonts with. Accents. Euro. First by Anthony Robinson. in Techno. Sci-fi 12,112 downloads 3 yesterday. 100% Free Download Donate to author. Newcastle by Anthony Robinson. in Fancy. Decorative 15,203 downloads 3 yesterday. 100% Free - 2 font files Download Donate to author. New York Punk by Anthony Robinson. in Fancy. Destroy 20,765 downloads 3 yesterday. 100% Free Download Donate to author. Lasso Of T...
http://dafont.com/anthony-robinson.d2796?page=5&text=AUBURN
*  Phillip Phillips Ringtones Free Download - Ringtone Maker Audiko
... Sublime, Kendrick Lamar, DevilDriver, K. Michelle, Five Finger Death Punch. Mobile apps. Login. JavaScript must be enabled in order for you to use Audiko in standard view. A B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S. T. U. V. W. X. Y. Z. 0...9. Thousands of ringtones on Audiko.net. Here is a chance to download a free Phillip Phillips ringtones for your iPhone or mobile. Specify your favorite line from your favorite award-winning track - Beggin, Get Up Get Down, Unpack Your Heart, or any other track. You may download your ringtone both in the MP3 and m4a formats, supported by both mobile phones and iPhones. Just select a track, and choose free Phillip Phillips ringtones download option. Download Phillip Phillips Ringtones. Top free ringtones. Name. Gone, Gone, Gone. Home. Raging Fire. Alive Again. Face. Get Up Get Down. Searchlight. Volcano. U Got It Bad. We've Got Tonight. Albums. Behind the Light Deluxe Version. Phillip Phillips. Weve Got Tonight American Idol Performance Single. Phillip Phil...
http://audiko.net/ringtone/phillip-phillips
*  Coles Phillips
... 'Clarence Coles Phillips' October 1880June 13, 1927 was an American artist and illustrator who signed his early works 'C. Coles Phillips', but after 1911 worked under the abbreviated name, 'Coles Phillips'. He is known for his stylish images of women and a signature use of negative space in the paintings he created for advertisements and the covers of popular magazines. Early life Career Personal life Notes References External links. Early life. ' ' Michael Schau, Watson-Guptill, 1975, p. Image:Coles Phillips2 Life.jpg The work of Phillips quickly became popular with the 'Life' readers. Schau & Phillips 1975, p. Phillips' use of negative space allowed the viewer to "fill-in" the image; it also reduced printing costs for the magazine, as "the novelty of the technique and the striking design qualities masked the fact that 'Life' was getting by with single color or two-color covers in a day when full-color covers were de rigueur for the better magazines". Phillips worked in watercolor and always painted fro...
https://en.wikipedia.org/wiki/Coles_Phillips
*  Phillip Phillips News, Pictures, and Videos | TMZ.com
Idol Managers Claim John Mayer's ex-manager plotted to jack "American Idol" champ Phillip Phillips out from under his contract with the show -- according to a new lawsuit, but the bigger news is Phil is a READ MORE > - 9/21/2015 2:51 PM PDT. Phillip Phillips I Want to be Gone, Gone, Gone From My Idol Contract Phillip Phillips says "American Idol" producers are ruining his career ... American Idol Winner Phillip Phillips Mother Popped for DUI Phillip Phillips' mother Sheryl Phillips -- who appeared on several episodes of "American Idol" during Phillip's championship run last season -- was arrested for DUI earlier today, TMZ has READ MORE > - 963 days ago. READ MORE > - 1063 days ago. Phillip Phillips Family Idol Champ s Forcing Us To Sell Pawn Shop Phillip Phillips' family says the singer left them high and dry after winning "American Idol" -- refusing to help them out financially -- and now, they've been forced to sell their cherished READ MORE > - 1066 days ago. Phillip Phillips Crooks Smash In Pawn Shop, St...
http://tmz.com/person/phillip-phillips/?adid=cr_featured_saga_14
*  HERBICIDE-TOLERANT PLANTS - BASF AGROCHEMICAL PRODUCTS.B.V.
In addition, enzymes of this embodiment will also comprise one or more of a leucine, threonine, a valine, or alanine at position 1,781 Am, a glycine at position l,785 Am, a proline at position l,786 Am, an asparagine at position 1,811 Am, a proline at position l,824 Am, a cysteine or glycine at position l,999 Am, a cysteine or arginine at position 2,027 ' Am, a glycine at position 2,039 Am, an asparagine at position 2,041 Am, a phenylalanine, isoleucine or leucine at position 2,049 Am, a valine at position 2,059 Am, a leucine at position 2,074 Am, a leucine, isoleucine, methionine or additional valine at position 2,075 Am, a glycine or threonine at position 2,078 Am, a phenylalanine at position 2,079 Am, a glutamic acid at position 2,080 Am, a deletion at position 2,080 Am, a deletion at position 2,081 Am, an arginine, tryptophan, phenylalanine, glycine, histidine, lysine, leucine, serine, threonine, or valine at position 2,088 Am, a glutamic acid at position 2,095 Am, an alanine or serine at position 2,096 A...
http://sumobrain.com/patents/wipo/Herbicide-tolerant-plants/WO2011028836.html
*  Bimolecular fluorescence complementation
This was achieved by dissecting GFP into C- and N-terminal GFP fragments. In addition, this study was the first report of an ' in vivo ' technique, now known as the bimolecular fluorescence complementation BiFC assay, to provide insight into the structural basis of protein complex formation through detection of fluorescence caused by the assembly of fluorescent reporter protein fragments tethered to interacting proteins. Generally, YFP is recommended to serve as the reporter protein, cleaved at residue 155 N-terminal consisting of residues 1–154 and C-terminal consisting of residues 155–238 or residue 173 in particular, as these sets of fragments are highly efficient in their complementation when fused to many interacting proteins and they produce low levels fluorescence when fused to non-interacting proteins. It is suggested that each target protein is fused to both the N- and C-terminal fragments of the fluorescent reporter protein in turn, and that the fragments are fused at each of the N- and C-terminal e...
https://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation
*  Candice Olson Family Spaces ISBN 9781118276679 PDF epub | Candice Olson ebook | eBookMall
Candice Olson Family Spaces ISBN 9781118276679 PDF epub. Candice Olson ebook. eBookMall. Candice Olson Family Spaces. Candice Olson. Design : Interior Decorating. Digital Book format: ePub Adobe DRM. Buy ePub. List price: $ 19.99 Our price: $ 16.99. Popular TV star Candice Olson shows you how to create your dream family room Everybody wants their home's main living space to meet the needs of the whole family—and look great doing it. Chock full of tips and ideas, Candice Olson Family Spaces features hundreds of full-color, before-and-after photos of Candice's stunning family room makeovers. Explains how good design accommodates all members of the family and their daily activities Designs offer functionality, durability, and comfort without sacrificing style As a busy mother of two, Candice brings valuable insight to what a family really needs from the spaces they share Redecorating—or even just dreaming about decorating—can transform one's outlook on life, and Candice Olson Family Spaces is the catalyst to get...
http://ebookmall.com/ebook/candice-olson-family-spaces/candice-olson/9781118276679
*  Abner Phillips (1737-1813), p.2
Abner Phillips - Surry County, NC There were Phillips in Surry County, NC as early as 1771, according to tax lists. Lewis' Dist.: Abner PHILLIPS, 400 acres, 1 white poll. LEWIS to Abner PHILLIPS, 300 acres on north side of Yadkin River, east side Turkey Creek, for 75. LEWIS to Abner PHILLIPS, 100 acres on north side of Yadkin River at Turkey Creek, for 75. Phillips Dist.: Abner PHILLIPS, 400 acres, 2 white polls. 1790 Census, Surry Co., NC, p.186: ABNER PHILLIPS 3 white males under 16 2 white males over 16 3 white females no slaves Note: Abner's sons Cornelius PHILLIPS and Ephraim PHILLIPS are also listed in the 1790 Census of Surry Co., NC, p.183. 1800 Census, Surry Co., NC: ABNER PHILLIPS. 1810 Census, Surry Co., NC, p.193: ABNER PHILLIPS. In the name of God Amen this tenth day of November in year of our lord 1812 I ABNER PHILLIPS of Surry County State of North Carolina being sick and weak in Body but in perfect mind memory thanks be given to God For the Same Calling unto mind the mortality of my bo...
http://moonzstuff.com/phillips/abner1737_p2.html
*  "Dear Abby" Advice Columnist Pauline Phillips Dead at 94 - Us Weekly
... US Weekly. News. Photos. Style. Beauty. Moms. Body. Entertainment. Subscribe. Give a Gift. Us Weekly. Newsletter. Subscribe. ADVERTISEMENT. "Dear Abby" Advice Columnist Pauline Phillips Dead at 94. Celebrity News. Jan. Tweet. Columnist Abigail Van Buren attends Friends Of Mario Cuomo Fundraiser Dinner on November 30, 1989 at the Sheraton Center in New York City. Newspapers -- and readers -- the world over lost an icon Wednesday, Jan. 16, after "Dear Abby" advice columnist Pauline Phillips died in Minneapolis at the age of 94. Phillips had suffered from Alzheimer's disease for years, but was still dishing out advice to readers as recently as 2002, though her daughter Jeanne took on co-authoring duties in the mid-1990s. PHOTOS: Stars we lost in 2012. Phillips, who wrote under the pen name Abigail Van Buren, shared advice on everything from dating to snoring spouses for more than 40 years to a readership of more than 90 million newspaper readers. "They gave her a bunch of letters, thinking that they would n...
http://usmagazine.com/celebrity-news/news/dear-abby-advice-columnist-pauline-phillips-dead-at-94-2013171
*  Joanna Phillips | UCSF Medical Center
Joanna Phillips. UCSF Medical Center. About UCSF. Find a Doctor. Conditions Treatments. Conditions Treatments A-Z. Patient Education. Doctors. Find a Doctor. Getting to UCSF Medical Center. Careers at UCSF Medical Center. UCSF Medical Group. Home > Doctors > Joanna Phillips. Find a Doctor. Joanna Phillips, M.D. Joanna Phillips is a neuropathologist at UCSF Medical Center who helps diagnose neurologic disease through the examination of central nervous system tissue. As an academic neuropathologist, her goal is to improve the treatment of brain tumors through research and patient care. She completed a pathology residency at the University of Washington in Seattle, followed by a fellowship in neuropathology at UCSF. Phillips is a member of several professional organizations, including the American Society for Investigative Pathology, American Association of Neuropathologists, American Association for Cancer Research and Glycobiology Research and Training Program. Safaee M, Fakurnejad S, Bloch O, Clark AJ, Ivan M...
http://ucsfhealth.org/joanna.phillips
*  SMART: Secondary literature for GLUCA domain
smart secondary literature for gluca domain smart mode normal genomic schultz et al proc natl acad sci usa letunic et al nucleic acids res doi nar gku home setup faq about glossary what s new feedback secondary literature sources for gluca the following references were automatically generated lopez lc frazier ml su cj kumar a saunders gf mammalian pancreatic preproglucagon contains three glucagon related peptides proc natl acad sci u s a display abstract we have isolated cdna clones encoding bovine pancreatic preproglucagon twenty five putative preproglucagon clones were selected by screening clones of a fetal bovine pancreas cdna library with a synthetic oligodeoxynucleotide probe the probe was a mixture of synthetic base dna oligomers constructed to correspond to the six carboxyl terminal amino acids residues of mature glucagon restriction mapping of six of these clones suggested that they represented a single mrna species primary sequence analysis of one clone containing a base pair dna insert revealed tha...
http://smart.embl-heidelberg.de/smart/show_secondary.cgi?domain=GLUCA
*  viruses share common: Topics by Science.gov
... Toggle navigation. Topics by Science.gov Home. Site Map. Index. Alerts. Help. Contact Us. About. Sample records for viruses share common. 1 2. 3. 4. 5. . . To gain insights into the role and specificity of hepaciviral nonstructural protein 2 NS2, which is required for HCV polyprotein processing and particle morphogenesis, we investigated whether NS2 structural and functional features are conserved between HCV and GBV-B. We found that GBV-B NS2, like HCV NS2, has cysteine protease activity responsible for cleavage at the NS2/NS3 junction, and we experimentally confirmed the location of this junction within the viral polyprotein. A model for GBV-B NS2 membrane topology was experimentally established by determining the membrane association properties of NS2 segments fused to green fluorescent protein GFP and their nuclear magnetic resonance structures using synthetic peptides as well as by applying an N-glycosylation scanning approach. Similar glycosylation studies confirmed the HCV NS2 organization. Togeth...
http://science.gov/topicpages/v/viruses share common.html
*  VPR: Andrea Phillips thanks President Obama, military for her husband's rescue
... .  Archive Home .  VPR.net Home . Andrea Phillips thanks President Obama, military for her husband's rescue. 04/13/09 5:42PM By Ross Sneyd.  MP3 .  Download MP3 . AP Photo/Toby Talbot. Andrea Phillips is flanked by her son, Daniel, and daughter, Mariah. Host A day after her husband was rescued from Somali pirates, Andrea Phillips said she wanted to thank friends, officials and especially President Obama for their support during the crisis. In a short news conference in South Burlington today, Phillips also said she had also been able to speak with her husband by telephone. VPR's Ross Sneyd has more. Sneyd Andrea Phillips has had the flu since a day before her husband's capture - and she's just now recovering. Since last week, when it became clear that Richard Phillips had given himself up to the pirates to protect the crew on his Merchant Marine ship, she's stayed out of the spotlight. She said her husband got a laugh out of her condition, knowing that she would be appeari...
http://vpr.net/news_detail/84680/andrea-phillips-thanks-president-obama-military-fo/
*  Protein superfamily
A 'protein superfamily' is the largest grouping clade of proteins for which common ancestry can be inferred see homology. Usually this common ancestry is based on structural alignment. and mechanistic similarity even though no sequence similarity is evident. 2 Superfamilies typically contain several protein families which show sequence similarity within the family. The term 'protein clan' is commonly used for protease superfamilies based on the MEROPS protease classification system. Identification Sequence homology. Structural homology. Mechanistic similarity. Evolutionary significance Examples Protein superfamily resources See also References. Sequence homology. sequence homology. Superfamily members typically show no detectable sequence homology. In the PA clan of protease s, for example, not a single residue is conserved through the superfamily, not even those in the catalytic triad. Conversely, the individual families that make up a superfamily are defined on the basis of their sequence alignment, for exa...
https://en.wikipedia.org/wiki/Protein_superfamily
*  Publications | NEB
FEBS Lett. Proc.Natl.Acad.Sci.USA J Parasitol. Proteins Mol.Cell.Biol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Protein Sci Proc. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J B...
https://neb.com/research/publications?page=3&start=30&num=10&sorting=Year&asc=False
*  Publications | NEB
Proc.Natl.Acad.Sci.USA Molecular Paradigms for Eradicating Helmenthic Parasites Exp.Parasitol. Appl.Environ.Microbiol., Trop.Med.Parasitol. Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Biochem J. NY Proc.Natl.Acad.Sci. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbio...
https://neb.com/research/publications?num=50&sorting=&asc=False
*  Publications | NEB
FEBS Lett. Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology B...
https://neb.com/sitecore/content/nebsg/home/research/publications?Page=2&sorting=Year
*  Par stability determinant
... notoc the par stability determinant is a bp locus of the pad plasmid which encodes a type i toxin antitoxin system in enterococcus faecalis it was the first such plasmid addiction module to be found in gram positive bacteria the par locus contains two gene s fst which encodes a amino acid toxic protein and a gene for rnaii the small rna anti toxin which inhibits fst translation the two genes are found on opposite dna strands and share a region which is where they are thought to have an antisense interaction their rna secondary structure s have been predicted computationally the complementary regions appear to be presented on exposed loops for interaction par maintains pad by means of post segregational killing psk if a daughter cell does not inherit the par locus the unstable rnaii will quickly degrade leaving the long lived fst toxin to damage or kill the daughter cell see also references further reading external links see also hok sok system references further reading external links category antisense ...
https://en.wikipedia.org/wiki/Par_stability_determinant
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?Page=29&asc=False
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?Page=55
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/research/publications?page=50&start=500&num=10
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/sitecore/content/nebsg/home/research/publications?Page=47
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. J Bacteriol Biotechnology Journal Biomedical Optics Express Nucleic Acids Res. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front ...
https://neb.com/sitecore/content/nebsg/home/research/publications?page=52&start=520&num=10&asc=False
*  .. Post navigation .. Happy Birthday Duncan Phillips .. Post navigation
post navigation square dancing last chance o is for origin story happy birthday duncan phillips posted on june by sarah osborne bender clockwise from top left milton avery black sea oil on canvas x in the phillips collection washington d c acquired robert motherwell in white and yellow ochre oil charcoal ink tempera and paper collage on paper x in the phillips collection washington d c acquired alexander calder only only bird tin cans and wire x x in the phillips collection washington d c acquired karl knaths the blue heron at the tide wash oil on canvas x in the phillips collection washington d c acquired i had the pleasure of speaking last night to alice phillips swistel great niece of duncan phillips who was born today in it s not surprising that the conversation came around to her memories of him though i ve worked here for over five years and handled many of phillips s belongings his j...
http://blog.phillipscollection.org/2012/06/26/happy-birthday-duncan-phillips/
*  Consensus sequence
In molecular biology and bioinformatics, the 'consensus sequence' is the calculated order of most frequent residues, either nucleotide or amino acid, found at each position in a sequence alignment. It represents the results of multiple sequence alignment s in which related sequences are compared to each other and similar sequence motifs are calculated. Biological significance Sequence analysis Notation. Thus a consensus sequence is a model for a putative DNA binding site : it is obtained by aligning all known examples of a certain recognition site and defined as the idealized sequence that represents the predominant base at each position. Any mutation allowing a mutated nucleotide in the core promoter sequence to look more like the consensus sequence is known as an 'up mutation'. On the contrary, mutations that destroy conserved nucleotides in the consensus sequence are known as 'down mutations'. These types of mutations down-regulate transcription since RNA polymerase can no longer bind as tightly to the cor...
https://en.wikipedia.org/wiki/Consensus_sequence
*  Publications | NEB
Proc.Natl.Acad.Sci.USA J Parasitol. Nature Structure Methods Mol.Biol. CABIOS Glycobiology Proceeding of the Heartworm Symposium Biotechnology Biochemistry Parasitology Today Science Archaea A Laboratory Manual Parasitology Proc Natl Acad Sci Clinical and Diagnostic Laboratory Immunology Abstracts Of Papers Of The American Chemical Society Accountability in Research Genetic Analysis Biomolecular Engineering Enzymes and Proteins from Hyperthermophilic Microorganisms, Advs.Protein Chemistry Genetics Society of America Nuc.Acids Res. Nucleic Acids Research BMC Res Notes J Steroid Biochem Mol Biol World J. Plant Cell Nature Chemistry Methods in Molecular Biology Mol Cell Biol. J Cell Sci BMC Microbiol Protein Cell Gene Ther Appl Microbiol Biotechnol Nat Biotechnol Front Physiol Appl Environ Microbiol Viruses Applications in Plant Sciences G3 Bethesda J Virol Anal Biochem AMB Express PLoS Pathog Curr Opin Chem Biol J Biol Eng Mol Biol Evol PLoS Genet Biology Basel Front Genet Curr Protoc Mol Biol Methods Mol Biol ...
https://neb.com/research/publications?num=100&sorting=Journal&asc=False

Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.List of strains of Escherichia coli: Escherichia coli is a well studied bacterium that was first identified by Theodor Escherich, after whom it was later named.Coles PhillipsProtein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Escherichia coli (molecular biology): Escherichia coli (; commonly abbreviated E. coli) is a gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms).Exogenous bacteria: Exogenous bacteria are microorganisms introduced to closed biological systems from the external world. They exist in aquatic and terrestrial environments, as well as the atmosphere.GA module: In molecular biology, the GA module, or protein G-related albumin-binding module, is a protein domain which occurs on the surface of numerous Gram-positive bacterial pathogens. Protein G of group C and G Streptococci interacts with the constant region of IgG and with human serum albumin.Bacterial outer membraneAbomasitis: Abomasitis (abomasal bloat) is a relatively rare ruminant disease characterized by inflammation of abomasum in young calves, lambs, and goat kids. It occurs with gastroenteritis but is seldom diagnosed as a separate condition, and its causes are as yet unknown.Anaerobacter: Anaerobacter are a genus of Gram-positive bacteria related to Clostridium. They are anaerobic chemotrophs and are unusual spore-formers as they produce more than one spore per bacterial cell (up to five spores).Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.FERM domain: In molecular biology, the FERM domain (F for 4.1 protein, E for ezrin, R for radixin and M for moesin) is a widespread protein module involved in localising proteins to the plasma membrane.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Trimeric autotransporter adhesin: In molecular biology, trimeric autotransporter adhesins (TAAs), are proteins found on the outer membrane of Gram-negative bacteria. Bacteria use TAAs in order to infect their host cells via a process called cell adhesion.AB toxin: The AB toxins are two-component protein complexes secreted by a number of pathogenic bacteria. They can be classified as Type III toxins because they interfere with internal cell function.Prokaryotic elongation factorsProximity ligation assay: Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions and modifications with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution and objectively quantified in unmodified cells and tissues.Reaction coordinateProtein synthesis inhibitor: A protein synthesis inhibitor is a substance that stops or slows the growth or proliferation of cells by disrupting the processes that lead directly to the generation of new proteins.CS-BLASTLegionella pneumophila: Legionella pneumophila is a thin, aerobic, pleomorphic, flagellated, non-spore forming, Gram-negative bacterium of the genus Legionella. L.Secretion: Secretion is the process of elaborating, releasing, and oozing chemicals, or a secreted chemical substance from a cell or gland. In contrast to excretion, the substance may have a certain function, rather than being a waste product.BacitracinGating signal: A gating signal is a digital signal or pulse (sometimes called a "trigger") that provides a time window so that a particular event or signal from among many will be selected and others will be eliminated or discarded.Membrane protein: Membrane proteins are proteins that interact with biological membranes. They are one of the common types of protein along with soluble globular proteins, fibrous proteins, and disordered proteins.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.Multidrug-resistant gram-negative bacteria: MDRGN bacteria is an abbreviation for multidrug resistant gram-negative bacteria. For hospitalized patients, and especially patients in intensive care units, these bacterial infections pose a serious and (as of 2010) rapidly emerging threat.Transfer-messenger RNA: Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1.CDP-abequose synthase: CDP-abequose synthase (, rfbJ (gene)) is an enzyme with system name CDP-alpha-D-abequose:NADP+ 4-oxidoreductase. This enzyme catalyses the following chemical reactionChloramphenicol acetyltransferaseTriparental mating: Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis.Listeria monocytogenes: Listeria monocytogenes is the bacterium that causes the infection listeriosis. It is a facultative anaerobic bacterium, capable of surviving in the presence or absence of oxygen.Cell membraneNitrogen deficiencyTripartite ATP-independent periplasmic transporter: Tripartite ATP-independent periplasmic transporters (TRAP transporters) are a large family of solute transporters found in bacteria and archaea, but not in eukaryotes, that appear to be specific for the uptake of organic acids. They are unique in that they utilize a substrate binding protein (SBP) in combination with a secondary transporter.SEA Native Peptide LigationSporulation in Bacillus subtilis: Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore. B.Phase problem: In physics the phase problem is the name given to the problem of loss of information concerning the phase that can occur when making a physical measurement. The name itself comes from the field of x-ray crystallography, where the phase problem has to be solved for the determination of a structure from diffraction data.LinezolidOxymonad: The Oxymonads are a group of flagellated protozoa found exclusively in the intestines of termites and other wood-eating insects. Along with the similar parabasalid flagellates, they harbor the symbiotic bacteria that are responsible for breaking down cellulose.Margaret Jope: Margaret Jope (1913–2004) was a Scottish biochemist, born as Henrietta Margaret Halliday in Peterhead, Scotland.Cholesterol-dependent cytolysin: Cholesterol-dependent cytolysins (CDC) are a family of β-barrel pore-forming exotoxins that are secreted by Gram-positive bacteria. CDC are secreted as water-soluble monomers of 50-70 kDa, that once bound to the target cell, will form a circular homo-oligomeric complex containing up to 40 monomers, although it is possible that some may incorporate more monomers.SaPI: SaPIs (Staphylococcus aureus or superantigen pathogenicity islands) are a family of mobile genetic elements resident in the genome of some strains of Staphylococcus aureus. Much like bacteriophages, SaPIs can be transferred to uninfected cells and integrate into the host chromosome.Translational regulation: Translational regulation refers to the control of the levels of protein synthesized from its mRNA. The corresponding mechanisms are primarily targeted on the control of ribosome recruitment on the initiation codon, but can also involve modulation of the elongation or termination of protein synthesis.DNA binding site: DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from other binding sites in that (1) they are part of a DNA sequence (e.Database of protein conformational diversity: The Database of protein conformational diversity (PCDB) is a database of diversity of protein tertiary structures within protein domains as determined by X-ray crystallography. Proteins are inherently flexible and this database collects information on this subject for use in molecular research.Gentamicin protection assay: The gentamicin protection assay or survival assay or invasion assay is a method used in microbiology. It is used to quantify the ability of pathogenic bacteria to invade eukaryotic cells.Plant Proteome Database: The Plant Proteome Database is a National Science Foundation-funded project to determine the biological function of each protein in plants.Sun Q, Zybailov B, Majeran W, Friso G, Olinares PD, van Wijk KJ.Virulence: Virulence is, by MeSH definition, the degree of pathogenicity within a group or species of parasites as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenicity of an organism - its ability to cause disease - is determined by its virulence factors.Lactic acid fermentationBurst kinetics: Burst kinetics is a form of enzyme kinetics that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase".Proteinogenic amino acid: Proteinogenic amino acids are amino acids that are precursors to proteins, and are incorporated into proteins cotranslationally — that is, during translation. There are 23 proteinogenic amino acids in prokaryotes (including N-Formylmethionine, mainly used to initiate protein synthesis and often removed afterward), but only 21 are encoded by the nuclear genes of eukaryotes.Molecular evolution: Molecular evolution is a change in the sequence composition of cellular molecules such as DNA, RNA, and proteins across generations. The field of molecular evolution uses principles of evolutionary biology and population genetics to explain patterns in these changes.Protein subcellular localization prediction: Protein subcellular localization prediction (or just protein localization prediction) involves the computational prediction of where a protein resides in a cell.Short linear motifXyloglucan endotransglucosylase: Xyloglucan endotransglucosylase (XET) is an apoplastic enzyme found across the plant kingdom. The enzyme catalyzes the endotransglucosylation of two xyloglucan polysaccharides, effectively 'stitching' them together.DimethylacetamideMediated transportGyrA RNA motif: The gyrA RNA motif is a conserved RNA structure identified by bioinformatics. The RNAs are present in multiple species of bacteria within the order Pseudomonadales.Internal ribosome entry site: An internal ribosome entry site, abbreviated IRES, is a nucleotide sequence that allows for translation initiation in the middle of a messenger RNA (mRNA) sequence as part of the greater process of protein synthesis. Usually, in eukaryotes, translation can be initiated only at the 5' end of the mRNA molecule, since 5' cap recognition is required for the assembly of the initiation complex.Food vacuole: The food vacuole, or digestive vacuole, is an organelle found in parasites that cause malaria. During the stage of the parasites' lifecycle where it resides within a human (or other mammalian) red blood cell, it is the site of haemoglobin digestion and the formation of the large haemozoin crystals that can be seen under a light microscope.AAA proteins: For other uses see AAA (disambiguation)PuromycinHuman Proteinpedia: Human Proteinpedia is a portal for sharing and integration of human proteomic data,.Kandasamy et al.Proteomics Standards Initiative: The Proteomics Standards Initiative (PSI) is a working group of Human Proteome Organization. It aims to define data standards for proteomics in order to facilitate data comparison, exchange and verification.Chaperone (protein): 250px|right|thumb|A top-view of the [[GroES/GroEL bacterial chaperone complex model]]Beef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.ThiazolineAlkaliphile: Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline (pH roughly 8.5-11) environments, growing optimally around a pH of 10.MIPModDB: MIPModDB is a database of comparative protein structure models of MIP (Major intrinsic proteins) family of proteins.Ammonia transporterMatrix model: == Mathematics and physics ==Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.

(1/65263) The Drosophila kismet gene is related to chromatin-remodeling factors and is required for both segmentation and segment identity.

The Drosophila kismet gene was identified in a screen for dominant suppressors of Polycomb, a repressor of homeotic genes. Here we show that kismet mutations suppress the Polycomb mutant phenotype by blocking the ectopic transcription of homeotic genes. Loss of zygotic kismet function causes homeotic transformations similar to those associated with loss-of-function mutations in the homeotic genes Sex combs reduced and Abdominal-B. kismet is also required for proper larval body segmentation. Loss of maternal kismet function causes segmentation defects similar to those caused by mutations in the pair-rule gene even-skipped. The kismet gene encodes several large nuclear proteins that are ubiquitously expressed along the anterior-posterior axis. The Kismet proteins contain a domain conserved in the trithorax group protein Brahma and related chromatin-remodeling factors, providing further evidence that alterations in chromatin structure are required to maintain the spatially restricted patterns of homeotic gene transcription.  (+info)

(2/65263) Molecular chaperones: small heat shock proteins in the limelight.

Small heat shock proteins have been the Cinderellas of the molecular chaperone world, but now the crystal structure of a small heat shock protein has been solved and mutation of two human homologues implicated in genetic disease. Intermediate filaments appear to be one of the key targets of their chaperone activity.  (+info)

(3/65263) Insect evolution: Redesigning the fruitfly.

Homeotic mutations in Drosophila can result in dramatic phenotypes that suggest the possibility for rapid morphological evolution, but dissection of the genetic pathway downstream of Ultrabithorax is beginning to reveal how wing morphology may have evolved by more gradual transformations.  (+info)

(4/65263) Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation.

The telomerase reverse transcriptase component (TERT) is not expressed in most primary somatic human cells and tissues, but is upregulated in the majority of immortalized cell lines and tumors. Here, we identify the c-Myc transcription factor as a direct mediator of telomerase activation in primary human fibroblasts through its ability to specifically induce TERT gene expression. Through the use of a hormone inducible form of c-Myc (c-Myc-ER), we demonstrate that Myc-induced activation of the hTERT promoter requires an evolutionarily conserved E-box and that c-Myc-ER-induced accumulation of hTERT mRNA takes place in the absence of de novo protein synthesis. These findings demonstrate that the TERT gene is a direct transcriptional target of c-Myc. Since telomerase activation frequently correlates with immortalization and telomerase functions to stabilize telomers in cycling cells, we tested whether Myc-induced activation of TERT gene expression represents an important mechanism through which c-Myc acts to immortalize cells. Employing the rat embryo fibroblast cooperation assay, we show that TERT is unable to substitute for c-Myc in the transformation of primary rodent fibroblasts, suggesting that the transforming activities of Myc extend beyond its ability to activate TERT gene expression and hence telomerase activity.  (+info)

(5/65263) A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA.

The nature of the broad substrate specificity phenomenon, as manifested by multidrug resistance proteins, is not yet understood. In the Escherichia coli multidrug transporter, MdfA, the hydrophobicity profile and PhoA fusion analysis have so far identified only one membrane-embedded charged amino acid residue (E26). In order to determine whether this negatively charged residue may play a role in multidrug recognition, we evaluated the expression and function of MdfA constructs mutated at this position. Replacing E26 with the positively charged residue lysine abolished the multidrug resistance activity against positively charged drugs, but retained chloramphenicol efflux and resistance. In contrast, when the negative charge was preserved in a mutant with aspartate instead of E26, chloramphenicol recognition and transport were drastically inhibited; however, the mutant exhibited almost wild-type multidrug resistance activity against lipophilic cations. These results suggest that although the negative charge at position 26 is not essential for active transport, it dictates the multidrug resistance character of MdfA. We show that such a negative charge is also found in other drug resistance transporters, and its possible significance regarding multidrug resistance is discussed.  (+info)

(6/65263) Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast.

Cell division in a number of eukaryotes, including the fission yeast Schizosaccharomyces pombe, is achieved through a medially placed actomyosin-based contractile ring. Although several components of the actomyosin ring have been identified, the mechanisms regulating ring assembly are still not understood. Here, we show by biochemical and mutational studies that the S.pombe actomyosin ring component Cdc4p is a light chain associated with Myo2p, a myosin II heavy chain. Localization of Myo2p to the medial ring depended on Cdc4p function, whereas localization of Cdc4p at the division site was independent of Myo2p. Interestingly, the actin-binding and motor domains of Myo2p are not required for its accumulation at the division site although the motor activity of Myo2p is essential for assembly of a normal actomyosin ring. The initial assembly of Myo2p and Cdc4p at the division site requires a functional F-actin cytoskeleton. Once established, however, F-actin is not required for the maintenance of Cdc4p and Myo2p medial rings, suggesting that the attachment of Cdc4p and Myo2p to the division site involves proteins other than actin itself.  (+info)

(7/65263) Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation.

The proton motive force (PMF) renders protein translocation across the Escherichia coli membrane highly efficient, although the underlying mechanism has not been clarified. The membrane insertion and deinsertion of SecA coupled to ATP binding and hydrolysis, respectively, are thought to drive the translocation. We report here that PMF significantly decreases the level of membrane-inserted SecA. The prlA4 mutation of SecY, which causes efficient protein translocation in the absence of PMF, was found to reduce the membrane-inserted SecA irrespective of the presence or absence of PMF. The PMF-dependent decrease in the membrane-inserted SecA caused an increase in the amount of SecA released into the extra-membrane milieu, indicating that PMF deinserts SecA from the membrane. The PMF-dependent deinsertion reduced the amount of SecA required for maximal translocation activity. Neither ATP hydrolysis nor exchange with external SecA was required for the PMF-dependent deinsertion of SecA. These results indicate that the SecA deinsertion is a limiting step of protein translocation and is accelerated by PMF, efficient protein translocation thereby being caused in the presence of PMF.  (+info)

(8/65263) Cloning and characterisation of a novel ompB operon from Vibrio cholerae 569B.

The ompB operon of Vibrio cholerae 569B has been cloned and fully sequenced. The operon encodes two proteins, OmpR and EnvZ, which share sequence identity with the OmpR and EnvZ proteins of a variety of other bacteria. Although the order of the ompR and envZ genes of V. cholerae is similar to that of the ompB operon of E. coli, S. typhimurium and X. nematophilus, the Vibrio operon exhibits a number of novel features. The structural organisation and features of the V. cholerae ompB operon are described.  (+info)


Which source of proteins is better the grains source or the animals products source?


Can we live on grains proteins putting aside animals proteins? and which one is better?
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I'd say grains because animal proteins such as meat, milk and eggs are all high in cholesterol, while plant foods usually have no or little cholesterol. Also most of the cholesterol you'll find in plant food is the good type of cholesterol, while animal products have the heart-clogging type.
Animal products can cause a lot of heath issues as well. The most common ones are bowel cancer and heart disease. 
Legumes and veggies are high in protein and extremely good for you while the animal proteins will give you sufficient protein but can cause health issues as well (particularly bowel problems)


What's the difference between these proteins found in shampoo and conditioner?


hydrolzed milk protein
hydrolzed silk protein
hydrolzed wheat protein
hydrolzed corn protein
hydrolzed soy protein
hydrolzed vegetable protein
keratin amino acids
vegetable amino acids

Do they work differently on the hair? 
What is best? Are there any better proteins not listed?
Can you over use proteins in the hair?
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Your hair is nothing but keratin protien and amino acids. Washing and chemical treatments depletes the hair of some of the nutirients.

All the protiens lists above are just derivatives of natural vegitation. They are perfectly fine for replenishing what your hair needs....just make sure you have a good balance of moisture as well.

As with anything you can overuse protiens. However, professional products are made with a certain ratio of ingredients (protiens, moisture, amino acids)....so it's pretty hard to OD on them...;)  Cheap grocery store shampoos are more interested in listing impressive looking ingredients without too much care on what it actually does to the hair.

Put it this way.....you have use professeional products....you have used the cheap stuff....what made your hair feel better??? :)


What are a few good vegetarian protein combos?


I have read that eating one plant protein alone is sometimes not sufficient but mixing vegetable proteins is more effective. I'm working my way slowly into being a vegetarian, what are some food combinations I can use? I'm really into different beans and types of rice right now too.
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The theory that you need "complete" proteins (like those found in meat) or need to combine different types of proteins in one meal has been disproved.

This theory was based upon the assumption that your body needs all essential amino acids at once in order to build proteins. However, it has since been shown that your body is capable of storing amino acids between meals. As long as you consume a variety of protein sources you will be fine!

Also, you should be aware that protein intake is not usually an issue for a vegetarian or vegan as long as they eat a varied diet.

Beans, nuts, legumes, grains, tofu and more are all fantastic sources of protein! 

If you are worried about your protein intake I suggest you take a look at the following website. It lists common plant-based foods and their protein content: http://www.vegparadise.com/protein.html

For more info on the myth of protein combining/complete proteins visit:
http://en.wikipedia.org/wiki/Protein_combining
http://findarticles.com/p/articles/mi_m0820/is_n211/ai_17010257

Hope this helped, and best of luck in your journey to vegetarianism :)


Is there a difference between proteins that is important to consider when becoming vegetarian?


I am becoming a vegetarian, and I am wondering if there is a difference between protein found in food and protein found in things like protein drinks/powders. 

Obviously, I know it is important to get the right amount of protein, but I am unsure of whether protein that I would get out of things like peanut butter/dairy would be better than out of a powder?

Am I making sense here?
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Yes. 

Proteins are made up of amino acids. There are eight (or nine - I can't remember) 'essential' amino acids, and (put simply) the more there are in a food, the better the protein.

There is only one food that contains all eight or nine amino acids in perfect balance - the best protein in existence.

Quinoa - a grain. Completely vegetarian.

EDIT:
You might also find it useful to read this:
http://1stholistic.com/nutrition/hol_nutr_protein.htm

The thing to remember if you're going to try vegetarianism is that you can't just cut out the meat and hope the dairy is enough - you have to replace the meat with other protein rich foods, such as beans, seeds, nuts and grains.

Watch this too - I just saw someone else recommend it and it's got loads of useful info that should answer your questions:
http://www.youtube.com/watch?v=Pjf-r3c4L1M


What do trace proteins in urine indicate if there are no other symptoms of anything?


For the last two prenatal visits (so 4 weeks) I've had trace proteins.  Very normal BP, no sudden weight gain, no swelling, no headaches or visual disturbances.  I had all of these with the proteins with my last pregnancy.  This time I have just the proteins.

I've been tested for a UTI but I don't have one.  What gives?  Is it an indicator of something bad that could happen between now and my due date?  I'm 34.5 weeks now.
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Just a trace of protein is nothing to worry about, especially with the normal bp, until it starts getting to +2-4 they won't be concerned. I had high bp my whole pregnancy and usually had trace or +1 but I was never diagnosed with pre eclampsia,


How much protein is in one egg white? Is there any protein in the yolk?


How much protein is in one egg white? Is there any protein in the yolk?
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An egg white (raw) contains about 3.6 grams of protein. The egg yolk (also raw) contains about 2.7 grams of protein.


What foods containing protein would go well with a tuna bake?


Ok, so for my science homework i have to create a menu that contains a balanced diet. Including  Carbohydrates, Proteins, Vitamins, Minerals, Fats and Water. I have made my starter which is a vegetable soup ( containing carbs, water, vitamins+minerals ) For my main i have done a tuna bake, but i dont really know what i can add to that to make it have more protein. Please help !      P.s, It would really help if you had a balanced pudding and drink for me aswell !
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The protein count of tuna casserole can be increased by adding peas, chopped boiled eggs, cheese, and doubling the tuna or adding the same amount of canned crab as the amount of tuna.


How much protein powder should I add to muffin recipes to make them like the store-bought bars?


I love the protein bars you can buy in stores.  But I have yet to find a recipe to make one that looks good.  Does anyone have any favorite recipes that taste like the protein bars at the store?

Also if I make my own by using a normal muffin recipe, how much protein powder should I add to the batch?  I want them high protein and low calorie/low fat.
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I try to get a lot of protein in my diet so I do a lot of experimenting with protein powders that you can add to other recipes.  I usually start with two servings of whatever protein powder I have and add it to the recipe.  You may have to play around with the recipe to get the correct dry ingredient/wet ingredient ratio.  

I also make these bran muffins.

http://www.cupcakerecipes.com/bran-muffin-recipe.html

They are high in protein and REALLY good.  I use either wheat bran or oat bran.  You can also eat them with peanut butter to add more protein but you increase the calories quite a bit.  

Hope this helps. :)