Agar
Culture Media
Microbial Sensitivity Tests
Chromogenic Compounds
Evaluation Studies as Topic
Colony Count, Microbial
Bacteria
Staphylococcus
Enterobacteriaceae
Blood
Food Microbiology
Drug Resistance, Microbial
Feces
Bacteria, Anaerobic
Peptones
Staphylococcus aureus
Salmonella
Streptococcus
Candida
Water Microbiology
Cell Transformation, Neoplastic
Esculin
Cosmetics
United States Food and Drug Administration
The Jun kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. (1/1701)
We have previously found that epidermal growth factor (EGF) mediates growth through the Jun N-terminal kinase/stress-activated kinase (JNK/SAPK) pathway in A549 human lung carcinoma cells. As observed here, EGF treatment also greatly enhances the tumorigenicity of A549 cells, suggesting an important role for JNK in cancer cell growth (F. Bost, R. McKay, N. Dean, and D. Mercola, J. Biol. Chem. 272:33422-33429, 1997). Several isoforms families of JNK, JNK1, JNK2, and JNK3, have been isolated; they arise from alternative splicing of three different genes and have distinct substrate binding properties. Here we have used specific phosphorothioate oligonucleotides targeted against the two major isoforms, JNK1 and JNK2, to discriminate their roles in EGF-induced transformation. Multiple antisense sequences have been screened, and two high-affinity and specific candidates have been identified. Antisense JNK1 eliminated steady-state mRNA and JNK1 protein expression with a 50% effective concentration (EC50) of <0.1 microM but did not alter JNK2 mRNA or protein levels. Conversely, antisense JNK2 specifically eliminated JNK2 steady-state mRNA and protein expression with an EC50 of 0.1 microM. Antisense JNK1 and antisense JNK2 inhibited by 40 and 70%, respectively, EGF-induced total JNK activity, whereas sense and scrambled-sequence control oligonucleotides had no effect. The elimination of mRNA, protein, and JNK activities lasted 48 and 72 h following a single Lipofectin treatment with antisense JNK1 and JNK2, respectively, indicating sufficient duration for examining the impact of specific elimination on the phenotype. Direct proliferation assays demonstrated that antisense JNK2 inhibited EGF-induced doubling of growth as well as the combination of active antisense oligonucleotides did. EGF treatment also induced colony formation in soft agar. This effect was completely inhibited by antisense JNK2 and combined-antisense treatment but not altered by antisense JNK1 alone. These results show that EGF doubles the proliferation (growth in soft agar as well as tumorigenicity in athymic mice) of A549 lung carcinoma cells and that the JNK2 isoform but not JNK1 is utilized for mediating the effects of EGF. This study represents the first demonstration of a cellular phenotype regulated by a JNK isoform family, JNK2. (+info)Effect of different lots of Mueller-Hinton agar on the interpretation of the gentamicin susceptibility of Pseudomonas aeruginosa. (2/1701)
Population distributions and quality control data for strains of Pseudomonas aeruginosa tested for gentamicin susceptibility on six lots of Mueller-Hinton agar were analyzed. The lots of agar were used in three University of Washington hospitals from April 1975 through October 1977. The analyses indicated that the performance of members of the P. aeruginosa populations in each hospital closely followed the performance of the quality control strain, P. aeruginosa ATCC 27853, when tested on each lot of Mueller-Hinton medium. The variability of zone diameters with the P. aeruginosa populations and the quality control strain indicated that a fixed indeterminate range (13 to 16 mm) of gentamicin susceptibility was not applicable to these organisms as it was with the Enterobacteriaceae. Variability in gentamicin susceptibility results was demonstrated in both minimal inhibitory concentration and disk diffusion tests when eight selected P. aeruginosa strains and the quality control strain were tested on each lot of medium. This variation in susceptibility to gentamicin was not related to the total Ca(2+), Mg(2+), or Zn(2+) content of each lot of medium. The data demonstrated that a moving indeterminate range of gentamicin susceptibility, 3 to 6 mm below the mean zone diameter of the quality control strain, was a suitable criterion for strains tested on a single medium lot. These results illustrate the importance of defining stringent performance standards for media used in the susceptibility testing of P. aeruginosa with gentamicin and other aminoglycoside antibiotics. (+info)Diffusion through agar blocks of finite dimensions: a theoretical analysis of three systems of practical significance in microbiology. (3/1701)
A number of experimental methods in biology depend on the kinetics of diffusion of a substance through a gel. This paper reviews the diffusion equations, gives the experimental limitations for some useful cases, and presents computer simulations for cases that cannot be treated analytically. While double diffusion is not considered, three single-diffusion situations are treated. (1) Systems for the study of chemotaxis in the gliding bacterium Myxococcus xanthus. Experimental designs used for this in many cases in the literature were inappropriate and mathematical analysis of these is presented. (2) The development of gradient plates. The time necessary for vertical diffusion to become substantially complete and before diffusion in the direction of the original slant has proceeded significantly is calculated. (3) The application to antimicrobial disk susceptibility tests. The basis of the measurement of antibiotic sensitivities with disks containing antimicrobial agents, as routinely used in clinical microbiological and testing laboratories, is analysed and the limitations are assessed and improvements suggested. (+info)Quantitative studies on competitive activities of skin bacteria growing on solid media. (4/1701)
Earlier quantitative investigations of antagonism between skin bacteria were based on the use of liquid cultures, but a more realistic model has now been devised, based on the use of the surfaces of solid media. Pure or mixed inocula were spread evenly over suitable agar media in Petri dishes marked out with a standard grid. Growth curves were constructed from viable counts of the surface bacteria after they had been removed from excised squares of the agar media and dispersed. The method was highly reproducible, and competitive interactions were revealed more clearly than in studies with liquid media. An antibiotic-producing strain of Staphylococcus epidermidis (S6+) readily suppressed strains of Micrococcus, Corynebacterium and Streptococcus species. However, a Staphylococcus aureus strain which was less sensitive to the antibiotic effect of S6+ interacted in a complex manner, depending on the absolute and relative size of the S6+ inoculum. (+info)Mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells. (5/1701)
Mad protein has been shown as an antagonist of c-Myc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL-7404 cell line was investigated experimentally. An eukarryotic vector pCDNA III containing full ORF fragment of mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were cloned. Studies on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the mad-transfected cells were partially inhibited in comparison to control cells. Flow Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc25A, an important regulator gene of G0/G1 to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of serume. Thus, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL-7404 cells. (+info)Use of Dorset egg medium for maintenance and transport of Neisseria meningitidis and Haemophilus influenzae type b. (6/1701)
Studies of bacterial meningitis are hampered by the inability to maintain the viability of etiological agents during transport to reference laboratories. The long-term survival rate of 20 isolates of Neisseria meningitidis and Haemophilus influenzae type b (Hib) on Dorset egg medium, supplemented Columbia agar base medium, chocolate agar, and Amies medium was compared with that on 70% GC agar (chocolate) transport medium. N. meningitidis isolates were also inoculated onto 5% horse blood agar, and Hib was inoculated onto Haemophilus test medium. All of the N. meningitidis isolates remained viable on Dorset egg medium for 21 days; viability on the other media was poor after only 7 days. Recovery rates of Hib isolates were similar on Dorset egg and Haemophilus test media (100% after 21 days) and significantly better than on the other media. Dorset egg medium is inexpensive and easy to make and may be invaluable for studies of bacterial meningitis in developing countries. (+info)Variation in Microbial Identification System accuracy for yeast identification depending on commercial source of Sabouraud dextrose agar. (7/1701)
The accuracy of the Microbial Identification System (MIS; MIDI, Inc. ) for identification of yeasts to the species level was compared by using 438 isolates grown on prepoured BBL Sabouraud dextrose agar (SDA) and prepoured Remel SDA. Correct identification was observed for 326 (74%) of the yeasts cultured on BBL SDA versus only 214 (49%) of yeasts grown on Remel SDA (P < 0.001). The commercial source of the SDA used in the MIS procedure significantly influences the system's accuracy. (+info)Visualization and modelling of the thermal inactivation of bacteria in a model food. (8/1701)
A large number of incidents of food poisoning have been linked to undercooked meat products. The use of mathematical modelling to describe heat transfer within foods, combined with data describing bacterial thermal inactivation, may prove useful in developing safer food products while minimizing thermal overprocessing. To examine this approach, cylindrical agar blocks containing immobilized bacteria (Salmonella typhimurium and Brochothrix thermosphacta) were used as a model system in this study. The agar cylinders were subjected to external conduction heating by immersion in a water bath. They were then incubated, sliced open, and examined by image analysis techniques for regions of no bacterial growth. A finite-difference scheme was used to model thermal conduction and the consequent bacterial inactivation. Bacterial inactivation rates were modelled with values for the time required to reduce bacterial number by 90% (D) and the temperature increase required to reduce D by 90% taken from the literature. Model simulation results agreed well with experimental results for both bacteria, demonstrating the utility of the technique. (+info)Agar is a substance derived from red algae, specifically from the genera Gelidium and Gracilaria. It is commonly used in microbiology as a solidifying agent for culture media. Agar forms a gel at relatively low temperatures (around 40-45°C) and remains stable at higher temperatures (up to 100°C), making it ideal for preparing various types of culture media.
In addition to its use in microbiology, agar is also used in other scientific research, food industry, and even in some artistic applications due to its unique gelling properties. It is important to note that although agar is often used in the preparation of food, it is not typically consumed as a standalone ingredient by humans or animals.
Culture media is a substance that is used to support the growth of microorganisms or cells in an artificial environment, such as a petri dish or test tube. It typically contains nutrients and other factors that are necessary for the growth and survival of the organisms being cultured. There are many different types of culture media, each with its own specific formulation and intended use. Some common examples include blood agar, which is used to culture bacteria; Sabouraud dextrose agar, which is used to culture fungi; and Eagle's minimum essential medium, which is used to culture animal cells.
Microbial sensitivity tests, also known as antibiotic susceptibility tests (ASTs) or bacterial susceptibility tests, are laboratory procedures used to determine the effectiveness of various antimicrobial agents against specific microorganisms isolated from a patient's infection. These tests help healthcare providers identify which antibiotics will be most effective in treating an infection and which ones should be avoided due to resistance. The results of these tests can guide appropriate antibiotic therapy, minimize the potential for antibiotic resistance, improve clinical outcomes, and reduce unnecessary side effects or toxicity from ineffective antimicrobials.
There are several methods for performing microbial sensitivity tests, including:
1. Disk diffusion method (Kirby-Bauer test): A standardized paper disk containing a predetermined amount of an antibiotic is placed on an agar plate that has been inoculated with the isolated microorganism. After incubation, the zone of inhibition around the disk is measured to determine the susceptibility or resistance of the organism to that particular antibiotic.
2. Broth dilution method: A series of tubes or wells containing decreasing concentrations of an antimicrobial agent are inoculated with a standardized microbial suspension. After incubation, the minimum inhibitory concentration (MIC) is determined by observing the lowest concentration of the antibiotic that prevents visible growth of the organism.
3. Automated systems: These use sophisticated technology to perform both disk diffusion and broth dilution methods automatically, providing rapid and accurate results for a wide range of microorganisms and antimicrobial agents.
The interpretation of microbial sensitivity test results should be done cautiously, considering factors such as the site of infection, pharmacokinetics and pharmacodynamics of the antibiotic, potential toxicity, and local resistance patterns. Regular monitoring of susceptibility patterns and ongoing antimicrobial stewardship programs are essential to ensure optimal use of these tests and to minimize the development of antibiotic resistance.
Chromogenic compounds are substances that can be converted into a colored product through a chemical reaction. These compounds are often used in various diagnostic tests, including microbiological assays and immunoassays, to detect the presence or absence of a specific analyte (such as a particular bacterium, enzyme, or antigen).
In these tests, a chromogenic substrate is added to the sample, and if the target analyte is present, it will react with the substrate and produce a colored product. The intensity of the color can often be correlated with the amount of analyte present in the sample, allowing for quantitative analysis.
Chromogenic compounds are widely used in clinical laboratories because they offer several advantages over other types of diagnostic tests. They are typically easy to use and interpret, and they can provide rapid results with high sensitivity and specificity. Additionally, chromogenic assays can be automated, which can help increase throughput and reduce the potential for human error.
Anti-bacterial agents, also known as antibiotics, are a type of medication used to treat infections caused by bacteria. These agents work by either killing the bacteria or inhibiting their growth and reproduction. There are several different classes of anti-bacterial agents, including penicillins, cephalosporins, fluoroquinolones, macrolides, and tetracyclines, among others. Each class of antibiotic has a specific mechanism of action and is used to treat certain types of bacterial infections. It's important to note that anti-bacterial agents are not effective against viral infections, such as the common cold or flu. Misuse and overuse of antibiotics can lead to antibiotic resistance, which is a significant global health concern.
"Evaluation studies" is a broad term that refers to the systematic assessment or examination of a program, project, policy, intervention, or product. The goal of an evaluation study is to determine its merits, worth, and value by measuring its effects, efficiency, and impact. There are different types of evaluation studies, including formative evaluations (conducted during the development or implementation of a program to provide feedback for improvement), summative evaluations (conducted at the end of a program to determine its overall effectiveness), process evaluations (focusing on how a program is implemented and delivered), outcome evaluations (assessing the short-term and intermediate effects of a program), and impact evaluations (measuring the long-term and broad consequences of a program).
In medical contexts, evaluation studies are often used to assess the safety, efficacy, and cost-effectiveness of new treatments, interventions, or technologies. These studies can help healthcare providers make informed decisions about patient care, guide policymakers in developing evidence-based policies, and promote accountability and transparency in healthcare systems. Examples of evaluation studies in medicine include randomized controlled trials (RCTs) that compare the outcomes of a new treatment to those of a standard or placebo treatment, observational studies that examine the real-world effectiveness and safety of interventions, and economic evaluations that assess the costs and benefits of different healthcare options.
A "colony count" is a method used to estimate the number of viable microorganisms, such as bacteria or fungi, in a sample. In this technique, a known volume of the sample is spread onto the surface of a solid nutrient medium in a petri dish and then incubated under conditions that allow the microorganisms to grow and form visible colonies. Each colony that grows on the plate represents an individual cell (or small cluster of cells) from the original sample that was able to divide and grow under the given conditions. By counting the number of colonies that form, researchers can make a rough estimate of the concentration of microorganisms in the original sample.
The term "microbial" simply refers to microscopic organisms, such as bacteria, fungi, or viruses. Therefore, a "colony count, microbial" is a general term that encompasses the use of colony counting techniques to estimate the number of any type of microorganism in a sample.
Colony counts are used in various fields, including medical research, food safety testing, and environmental monitoring, to assess the levels of contamination or the effectiveness of disinfection procedures. However, it is important to note that colony counts may not always provide an accurate measure of the total number of microorganisms present in a sample, as some cells may be injured or unable to grow under the conditions used for counting. Additionally, some microorganisms may form clusters or chains that can appear as single colonies, leading to an overestimation of the true cell count.
Bacteria are single-celled microorganisms that are among the earliest known life forms on Earth. They are typically characterized as having a cell wall and no membrane-bound organelles. The majority of bacteria have a prokaryotic organization, meaning they lack a nucleus and other membrane-bound organelles.
Bacteria exist in diverse environments and can be found in every habitat on Earth, including soil, water, and the bodies of plants and animals. Some bacteria are beneficial to their hosts, while others can cause disease. Beneficial bacteria play important roles in processes such as digestion, nitrogen fixation, and biogeochemical cycling.
Bacteria reproduce asexually through binary fission or budding, and some species can also exchange genetic material through conjugation. They have a wide range of metabolic capabilities, with many using organic compounds as their source of energy, while others are capable of photosynthesis or chemosynthesis.
Bacteria are highly adaptable and can evolve rapidly in response to environmental changes. This has led to the development of antibiotic resistance in some species, which poses a significant public health challenge. Understanding the biology and behavior of bacteria is essential for developing strategies to prevent and treat bacterial infections and diseases.
Staphylococcus is a genus of Gram-positive, facultatively anaerobic bacteria that are commonly found on the skin and mucous membranes of humans and other animals. Many species of Staphylococcus can cause infections in humans, but the most notable is Staphylococcus aureus, which is responsible for a wide range of illnesses, from minor skin infections to life-threatening conditions such as pneumonia, endocarditis, and sepsis.
Staphylococcus species are non-motile, non-spore forming, and typically occur in grape-like clusters when viewed under a microscope. They can be coagulase-positive or coagulase-negative, with S. aureus being the most well-known coagulase-positive species. Coagulase is an enzyme that causes the clotting of plasma, and its presence is often used to differentiate S. aureus from other Staphylococcus species.
These bacteria are resistant to many commonly used antibiotics, including penicillin, due to the production of beta-lactamases. Methicillin-resistant Staphylococcus aureus (MRSA) is a particularly problematic strain that has developed resistance to multiple antibiotics and can cause severe, difficult-to-treat infections.
Proper hand hygiene, use of personal protective equipment, and environmental cleaning are crucial measures for preventing the spread of Staphylococcus in healthcare settings and the community.
Enterobacteriaceae is a family of gram-negative, rod-shaped bacteria that are commonly found in the intestines of humans and animals. Many species within this family are capable of causing various types of infections, particularly in individuals with weakened immune systems. Some common examples of Enterobacteriaceae include Escherichia coli (E. coli), Klebsiella pneumoniae, Proteus mirabilis, and Salmonella enterica.
These bacteria are typically characterized by their ability to ferment various sugars and produce acid and gas as byproducts. They can also be distinguished by their biochemical reactions, such as their ability to produce certain enzymes or resist specific antibiotics. Infections caused by Enterobacteriaceae can range from mild to severe, depending on the species involved and the overall health of the infected individual.
Some infections caused by Enterobacteriaceae include urinary tract infections, pneumonia, bloodstream infections, and foodborne illnesses. Proper hygiene, such as handwashing and safe food handling practices, can help prevent the spread of these bacteria and reduce the risk of infection.
Blood is the fluid that circulates in the body of living organisms, carrying oxygen and nutrients to the cells and removing carbon dioxide and other waste products. It is composed of red and white blood cells suspended in a liquid called plasma. The main function of blood is to transport oxygen from the lungs to the body's tissues and carbon dioxide from the tissues to the lungs. It also transports nutrients, hormones, and other substances to the cells and removes waste products from them. Additionally, blood plays a crucial role in the body's immune system by helping to fight infection and disease.
Food microbiology is the study of the microorganisms that are present in food, including bacteria, viruses, fungi, and parasites. This field examines how these microbes interact with food, how they affect its safety and quality, and how they can be controlled during food production, processing, storage, and preparation. Food microbiology also involves the development of methods for detecting and identifying pathogenic microorganisms in food, as well as studying the mechanisms of foodborne illnesses and developing strategies to prevent them. Additionally, it includes research on the beneficial microbes found in certain fermented foods and their potential applications in improving food quality and safety.
Microbial drug resistance is a significant medical issue that refers to the ability of microorganisms (such as bacteria, viruses, fungi, or parasites) to withstand or survive exposure to drugs or medications designed to kill them or limit their growth. This phenomenon has become a major global health concern, particularly in the context of bacterial infections, where it is also known as antibiotic resistance.
Drug resistance arises due to genetic changes in microorganisms that enable them to modify or bypass the effects of antimicrobial agents. These genetic alterations can be caused by mutations or the acquisition of resistance genes through horizontal gene transfer. The resistant microbes then replicate and multiply, forming populations that are increasingly difficult to eradicate with conventional treatments.
The consequences of drug-resistant infections include increased morbidity, mortality, healthcare costs, and the potential for widespread outbreaks. Factors contributing to the emergence and spread of microbial drug resistance include the overuse or misuse of antimicrobials, poor infection control practices, and inadequate surveillance systems.
To address this challenge, it is crucial to promote prudent antibiotic use, strengthen infection prevention and control measures, develop new antimicrobial agents, and invest in research to better understand the mechanisms underlying drug resistance.
Feces are the solid or semisolid remains of food that could not be digested or absorbed in the small intestine, along with bacteria and other waste products. After being stored in the colon, feces are eliminated from the body through the rectum and anus during defecation. Feces can vary in color, consistency, and odor depending on a person's diet, health status, and other factors.
Anaerobic bacteria are a type of bacteria that do not require oxygen to grow and survive. Instead, they can grow in environments that have little or no oxygen. Some anaerobic bacteria can even be harmed or killed by exposure to oxygen. These bacteria play important roles in many natural processes, such as decomposition and the breakdown of organic matter in the digestive system. However, some anaerobic bacteria can also cause disease in humans and animals, particularly when they infect areas of the body that are normally oxygen-rich. Examples of anaerobic bacterial infections include tetanus, gas gangrene, and dental abscesses.
Peptones are not a medical term per se, but they are commonly used in medical and clinical laboratory settings. Peptones are complex organic compounds that result from the partial hydrolysis of proteins. They consist of a mixture of polypeptides, peptides, and free amino acids.
In medical laboratories, peptones are often used as a nutrient source in various culture media for the growth of microorganisms such as bacteria and fungi. Peptone water is a common liquid medium used to culture and isolate bacteria. It contains peptones, sodium chloride, and other ingredients that provide essential nutrients for bacterial growth.
Peptones are also used in biochemical tests to identify specific microorganisms based on their ability to metabolize certain components of the peptone. For example, in the sulfur-indole-motility (SIM) medium, peptones serve as a source of amino acids and other nutrients that support the growth of bacteria producing enzymes responsible for the production of indole from tryptophan.
Staphylococcus aureus is a type of gram-positive, round (coccal) bacterium that is commonly found on the skin and mucous membranes of warm-blooded animals and humans. It is a facultative anaerobe, which means it can grow in the presence or absence of oxygen.
Staphylococcus aureus is known to cause a wide range of infections, from mild skin infections such as pimples, impetigo, and furuncles (boils) to more severe and potentially life-threatening infections such as pneumonia, endocarditis, osteomyelitis, and sepsis. It can also cause food poisoning and toxic shock syndrome.
The bacterium is often resistant to multiple antibiotics, including methicillin, which has led to the emergence of methicillin-resistant Staphylococcus aureus (MRSA) strains that are difficult to treat. Proper hand hygiene and infection control practices are critical in preventing the spread of Staphylococcus aureus and MRSA.
Salmonella is a genus of rod-shaped, Gram-negative bacteria that are facultative anaerobes and are motile due to peritrichous flagella. They are non-spore forming and often have a single polar flagellum when grown in certain conditions. Salmonella species are important pathogens in humans and other animals, causing foodborne illnesses known as salmonellosis.
Salmonella can be found in the intestinal tracts of humans, birds, reptiles, and mammals. They can contaminate various foods, including meat, poultry, eggs, dairy products, and fresh produce. The bacteria can survive and multiply in a wide range of temperatures and environments, making them challenging to control completely.
Salmonella infection typically leads to gastroenteritis, characterized by symptoms such as diarrhea, abdominal cramps, fever, and vomiting. In some cases, the infection may spread beyond the intestines, leading to more severe complications like bacteremia (bacterial infection of the blood) or focal infections in various organs.
There are two main species of Salmonella: S. enterica and S. bongori. S. enterica is further divided into six subspecies and numerous serovars, with over 2,500 distinct serotypes identified to date. Some well-known Salmonella serovars include S. Typhi (causes typhoid fever), S. Paratyphi A, B, and C (cause paratyphoid fever), and S. Enteritidis and S. Typhimurium (common causes of foodborne salmonellosis).
Oxacillin is a type of antibiotic known as a penicillinase-resistant penicillin. It is used to treat infections caused by bacteria that are resistant to other types of penicillins. Oxacillin is commonly used to treat infections of the skin, soft tissue, and bone.
Here is the medical definition of oxacillin:
Oxacillin is a semisynthetic antibiotic derived from penicillin that is resistant to staphylococcal penicillinases. It is used to treat infections caused by susceptible strains of staphylococci and some streptococci, including penicillinase-producing staphylococci. Oxacillin is available as a sterile powder for injection or as a oral capsule.
It is important to note that the overuse or misuse of antibiotics like oxacillin can lead to the development of antibiotic resistance, which makes infections harder to treat. It's essential to use antibiotics only when necessary and as directed by a healthcare professional.
Streptococcus is a genus of Gram-positive, spherical bacteria that typically form pairs or chains when clustered together. These bacteria are facultative anaerobes, meaning they can grow in the presence or absence of oxygen. They are non-motile and do not produce spores.
Streptococcus species are commonly found on the skin and mucous membranes of humans and animals. Some strains are part of the normal flora of the body, while others can cause a variety of infections, ranging from mild skin infections to severe and life-threatening diseases such as sepsis, meningitis, and toxic shock syndrome.
The pathogenicity of Streptococcus species depends on various virulence factors, including the production of enzymes and toxins that damage tissues and evade the host's immune response. One of the most well-known Streptococcus species is Streptococcus pyogenes, also known as group A streptococcus (GAS), which is responsible for a wide range of clinical manifestations, including pharyngitis (strep throat), impetigo, cellulitis, necrotizing fasciitis, and rheumatic fever.
It's important to note that the classification of Streptococcus species has evolved over time, with many former members now classified as different genera within the family Streptococcaceae. The current classification system is based on a combination of phenotypic characteristics (such as hemolysis patterns and sugar fermentation) and genotypic methods (such as 16S rRNA sequencing and multilocus sequence typing).
'Candida' is a type of fungus (a form of yeast) that is commonly found on the skin and inside the body, including in the mouth, throat, gut, and vagina, in small amounts. It is a part of the normal microbiota and usually does not cause any problems. However, an overgrowth of Candida can lead to infections known as candidiasis or thrush. Common sites for these infections include the skin, mouth, throat, and genital areas. Some factors that can contribute to Candida overgrowth are a weakened immune system, certain medications (such as antibiotics and corticosteroids), diabetes, pregnancy, poor oral hygiene, and wearing damp or tight-fitting clothing. Common symptoms of candidiasis include itching, redness, pain, and discharge. Treatment typically involves antifungal medication, either topical or oral, depending on the site and severity of the infection.
Water microbiology is not a formal medical term, but rather a branch of microbiology that deals with the study of microorganisms found in water. It involves the identification, enumeration, and characterization of bacteria, viruses, parasites, and other microscopic organisms present in water sources such as lakes, rivers, oceans, groundwater, drinking water, and wastewater.
In a medical context, water microbiology is relevant to public health because it helps to assess the safety of water supplies for human consumption and recreational activities. It also plays a critical role in understanding and preventing waterborne diseases caused by pathogenic microorganisms that can lead to illnesses such as diarrhea, skin infections, and respiratory problems.
Water microbiologists use various techniques to study water microorganisms, including culturing, microscopy, genetic analysis, and biochemical tests. They also investigate the ecology of these organisms, their interactions with other species, and their response to environmental factors such as temperature, pH, and nutrient availability.
Overall, water microbiology is a vital field that helps ensure the safety of our water resources and protects public health.
Neoplastic cell transformation is a process in which a normal cell undergoes genetic alterations that cause it to become cancerous or malignant. This process involves changes in the cell's DNA that result in uncontrolled cell growth and division, loss of contact inhibition, and the ability to invade surrounding tissues and metastasize (spread) to other parts of the body.
Neoplastic transformation can occur as a result of various factors, including genetic mutations, exposure to carcinogens, viral infections, chronic inflammation, and aging. These changes can lead to the activation of oncogenes or the inactivation of tumor suppressor genes, which regulate cell growth and division.
The transformation of normal cells into cancerous cells is a complex and multi-step process that involves multiple genetic and epigenetic alterations. It is characterized by several hallmarks, including sustained proliferative signaling, evasion of growth suppressors, resistance to cell death, enabling replicative immortality, induction of angiogenesis, activation of invasion and metastasis, reprogramming of energy metabolism, and evading immune destruction.
Neoplastic cell transformation is a fundamental concept in cancer biology and is critical for understanding the molecular mechanisms underlying cancer development and progression. It also has important implications for cancer diagnosis, prognosis, and treatment, as identifying the specific genetic alterations that underlie neoplastic transformation can help guide targeted therapies and personalized medicine approaches.
Esculin is a glucoside derived from the bark of willow trees and other plants. It has been used in scientific research as a substrate to test the activity of certain types of bacteria, particularly those that have the ability to produce an enzyme called beta-glucosidase. When esculin comes into contact with this enzyme, it is broken down and forms a chemical compound called esculetin, which can be detected and measured. This reaction is often used as a way to identify and study bacteria that produce beta-glucosidase.
Esculin is not typically used in medical treatments or therapies, but it may have some potential uses in the development of new drugs or diagnostic tools. As with any chemical compound, esculin should be handled with care and used only under the guidance of a trained professional.
Glossitis is a medical term that refers to inflammation of the tongue. This condition can cause symptoms such as swelling, redness, pain, and smoothness or discoloration of the tongue's surface. Glossitis can have various causes, including nutritional deficiencies (such as vitamin B12 or folate deficiency), allergic reactions, infections (bacterial, viral, or fungal), irritants (such as hot and spicy foods, alcohol, or tobacco), and autoimmune disorders (such as pemphigus vulgaris or lichen planus). Treatment for glossitis depends on the underlying cause.
Cosmetics are defined in the medical field as products that are intended to be applied or introduced to the human body for cleansing, beautifying, promoting attractiveness, and altering the appearance. According to the U.S. Food and Drug Administration (FDA), cosmetics include skin creams, lotions, makeup, perfumes, lipsticks, fingernail polishes, eye and facial makeup preparations, shampoos, permanent waves, hair colors, toothpastes, and deodorants, as well as any material intended for use as a component of a cosmetic product.
It's important to note that the FDA classifies cosmetics and drugs differently. Drugs are defined as products that are intended to diagnose, cure, mitigate, treat, or prevent disease, and/or affect the structure or function of the body. Some products, such as anti-dandruff shampoos or toothpastes with fluoride, can be considered both a cosmetic and a drug because they have both cleansing and therapeutic properties. These types of products are subject to regulation by both the FDA's Office of Cosmetics and Colors and its Center for Drug Evaluation and Research.
Cosmetics must not be adulterated or misbranded, meaning that they must be safe for use under labeled or customary conditions, properly packaged and labeled, and not contain any harmful ingredients. However, the FDA does not have the authority to approve cosmetic products before they go on the market, with the exception of color additives. Manufacturers are responsible for ensuring that their products are safe and properly labeled.
A laboratory (often abbreviated as lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurements may be performed. In the medical field, laboratories are specialized spaces for conducting diagnostic tests and analyzing samples of bodily fluids, tissues, or other substances to gain insights into patients' health status.
There are various types of medical laboratories, including:
1. Clinical Laboratories: These labs perform tests on patient specimens to assist in the diagnosis, treatment, and prevention of diseases. They analyze blood, urine, stool, CSF (cerebrospinal fluid), and other samples for chemical components, cell counts, microorganisms, and genetic material.
2. Pathology Laboratories: These labs focus on the study of disease processes, causes, and effects. Histopathology involves examining tissue samples under a microscope to identify abnormalities or signs of diseases, while cytopathology deals with individual cells.
3. Microbiology Laboratories: In these labs, microorganisms like bacteria, viruses, fungi, and parasites are cultured, identified, and studied to help diagnose infections and determine appropriate treatments.
4. Molecular Biology Laboratories: These labs deal with the study of biological molecules, such as DNA, RNA, and proteins, to understand their structure, function, and interactions. They often use techniques like PCR (polymerase chain reaction) and gene sequencing for diagnostic purposes.
5. Immunology Laboratories: These labs specialize in the study of the immune system and its responses to various stimuli, including infectious agents and allergens. They perform tests to diagnose immunological disorders, monitor immune function, and assess vaccine effectiveness.
6. Toxicology Laboratories: These labs analyze biological samples for the presence and concentration of chemicals, drugs, or toxins that may be harmful to human health. They help identify potential causes of poisoning, drug interactions, and substance abuse.
7. Blood Banks: Although not traditionally considered laboratories, blood banks are specialized facilities that collect, test, store, and distribute blood and its components for transfusion purposes.
Medical laboratories play a crucial role in diagnosing diseases, monitoring disease progression, guiding treatment decisions, and assessing patient outcomes. They must adhere to strict quality control measures and regulatory guidelines to ensure accurate and reliable results.
The United States Food and Drug Administration (FDA) is a federal government agency responsible for protecting public health by ensuring the safety, efficacy, and security of human and veterinary drugs, biological products, medical devices, our country's food supply, cosmetics, and products that emit radiation. The FDA also provides guidance on the proper use of these products, and enforces laws and regulations related to them. It is part of the Department of Health and Human Services (HHS).
Clinical laboratory techniques are methods and procedures used in medical laboratories to perform various tests and examinations on patient samples. These techniques help in the diagnosis, treatment, and prevention of diseases by analyzing body fluids, tissues, and other specimens. Some common clinical laboratory techniques include:
1. Clinical chemistry: It involves the analysis of bodily fluids such as blood, urine, and cerebrospinal fluid to measure the levels of chemicals, hormones, enzymes, and other substances in the body. These measurements can help diagnose various medical conditions, monitor treatment progress, and assess overall health.
2. Hematology: This technique focuses on the study of blood and its components, including red and white blood cells, platelets, and clotting factors. Hematological tests are used to diagnose anemia, infections, bleeding disorders, and other hematologic conditions.
3. Microbiology: It deals with the identification and culture of microorganisms such as bacteria, viruses, fungi, and parasites. Microbiological techniques are essential for detecting infectious diseases, determining appropriate antibiotic therapy, and monitoring the effectiveness of treatment.
4. Immunology: This technique involves studying the immune system and its response to various antigens, such as bacteria, viruses, and allergens. Immunological tests are used to diagnose autoimmune disorders, immunodeficiencies, and allergies.
5. Histopathology: It is the microscopic examination of tissue samples to identify any abnormalities or diseases. Histopathological techniques are crucial for diagnosing cancer, inflammatory conditions, and other tissue-related disorders.
6. Molecular biology: This technique deals with the study of DNA, RNA, and proteins at the molecular level. Molecular biology tests can be used to detect genetic mutations, identify infectious agents, and monitor disease progression.
7. Cytogenetics: It involves analyzing chromosomes and genes in cells to diagnose genetic disorders, cancer, and other diseases. Cytogenetic techniques include karyotyping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH).
8. Flow cytometry: This technique measures physical and chemical characteristics of cells or particles as they flow through a laser beam. Flow cytometry is used to analyze cell populations, identify specific cell types, and detect abnormalities in cells.
9. Diagnostic radiology: It uses imaging technologies such as X-rays, computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound to diagnose various medical conditions.
10. Clinical chemistry: This technique involves analyzing body fluids, such as blood and urine, to measure the concentration of various chemicals and substances. Clinical chemistry tests are used to diagnose metabolic disorders, electrolyte imbalances, and other health conditions.
Agar
Endo agar
Wes Agar
Agar dilution
Steve Agar
Agar, Gujarat
Agar (disambiguation)
Francilla Agar
John Agar
Agar-Ellis
James Agar
Bernice Agar
M17 agar
Agar Town
It-Agar
Nutrient agar
Ryan Agar
PNP agar
Sabouraud agar
R2A agar
Augustus Agar
Agar... If
Wyatt Agar
Herbert Agar
Agar Panchaitan
Richard Agar
Nicholas Agar
Agar Wynne
Amber Agar
MRS agar
Agar - Wikipedia
Bus from Blairsville to Agar
BAM Media M20: Blood Agar | FDA
Agar Grove Phase 1b
BAM Media M96: Mannitol Maltose Agar | FDA
Agar - Kroger
BD DIFCO™ Oatmeal Agar 500g - 255210 | BD
Oxford Listeria Agar | Neogen
Blogger: User Profile: Agar
Cara Mendidik hati anak agar tumbuh keikhlasan - Ustadz Abdul Kholik Djunaedi
Plate Mueller Hinton II Agar 100 Ea - 221275 | BD
Cara agar forum aman dari spam/spammer?
Agar Agar algae - Botanical online
Class 2 Device Recall BBL Trypticase Soy Agar with 5 Sheep Blood
Agar Efisien, Perusahaan Perlu Optimalkan Jasa Penerjemah Tersumpah
Agar Moisturizing Skincare Benefits - L'Oréal Paris
Contraindications of agar agar - Botanical online
Agar mix? - Advanced Mycology - Shroomery Message Board
Australia skipper Aaron Finch backing Ashton Agar to become T20 'finisher' | Cricket - Hindustan Times
Chocolate Agar
Agar feeling deja vu after nailbiter | Video | Watch TV Show | Sky Sports
Swallow Globe Agar-Agar Powder (Red Jelly Powder) - 1oz | 8991689168018
George Agar - Online Cenotaph - Auckland War Memorial Museum
Jerry Agar on "facts" : Warren Kinsella
Agar Artist Caitlin Cahak | Virology Blog
Tryptic Soy Agar | w Lecithin and Tween 80 | Neogen
Mohammad Rafi - Aap Yun Hi Agar | All The Lyrics
LB Agar plate, plain
Another word for NUTRIENT AGAR > Synonyms &...
Sabouraud-Dextrose Agar1
- Mix 65 grams of Sabouraud-Dextrose Agar with 1 liter of water. (schoolspecialty.com)
Eileen Agar3
- Presented to Tate Archive by Eileen Agar in 1989 and transferred from the photograph collection in 2012. (tate.org.uk)
- Artists Claude Cahun, Eileen Agar and Isabel Rawsthorne have in recent years enjoyed a revival as practitioners of exciting and experimental art forms. (citylit.ac.uk)
- Photographer, painter, sculptor and collagist, Eileen Agar was inexhaustibly curious about the world around her who regarded the flotsam and jetsam found on a beach no less important than oil paint or watercolour in her art. (citylit.ac.uk)
Tons of agar2
- Around the time of World War II, approximately 2,500 tons of agar were produced annually. (wikipedia.org)
- According to Santelices and Doty (1989) close to 5 000 tons of agar are processed annually from 25 000-30 000 tons of Gracilaria . (fao.org)
Autoclave1
- Add 15g agar to 400mL molecular grade H2O and autoclave for 20 minutes. (cdc.gov)
Microbiology3
- Agar was first described for use in microbiology in 1882 by the German microbiologist Walther Hesse, an assistant working in Robert Koch's laboratory, on the suggestion of his wife Fanny Hesse. (wikipedia.org)
- With its newfound use in microbiology, agar production quickly increased. (wikipedia.org)
- The theme for the Agar Art 2023 contest is " Microbiology in Space. (asm.org)
Petri2
- You may use any organism(s), with any type(s) of agar, on any size or shape petri dish. (asm.org)
- As long as researchers have been growing bacteria on Petri dishes using a jelly-like growth medium called agar, they have been struck by the interesting colors and growth patterns that microbes can produce from one experiment to the next. (nih.gov)
Overlays2
Microbes3
- Beginning in the late 19th century, agar began to be used as a solid medium for growing various microbes. (wikipedia.org)
- Agar quickly supplanted gelatin as the base of microbiological media, due to its higher melting temperature, allowing microbes to be grown at higher temperatures without the media liquefying. (wikipedia.org)
- Submissions can be either 'Traditional' (microbes on agar) or 'Open' (any form of media). (asm.org)
Synonym1
- Gracilaria edulis or its synonym G. lichenoides is specifically referred to as agal-agal or Ceylon agar. (wikipedia.org)
Stabilizer3
- Agar has that unique property of holding so much water in its gels (99.9%) so it is used widely for such gelling and stabilizer qualities not only in food but also in other industries. (fao.org)
- An improved stabilizer for icings is prepared by addition of 0.4 to 0.6% surface active agent to an agar level of 1.2 to 2.4% agar. (fao.org)
- The flat or sweet-roll icings are prepared by boiling the agar stabilizer in a sucrose-water solution, then adding this hot mixture to the icing sugar. (fao.org)
Gracilaria2
- Agar (/ˈeɪɡɑːr/ or /ˈɑːɡər/), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from "ogonori" (Gracilaria) and "tengusa" (Gelidiaceae). (wikipedia.org)
- The word agar comes from agar-agar, the Malay name for red algae (Gigartina, Eucheuma, Gracilaria) from which the jelly is produced. (wikipedia.org)
Agaropectin3
- As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. (wikipedia.org)
- The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. (wikipedia.org)
- Agar consists of a mixture of two polysaccharides: agarose and agaropectin, with agarose making up about 70% of the mixture, while agaropectin makes about 30% of it. (wikipedia.org)
Microorganisms1
- Submissions should be high-quality photos (at least 8' x 10' at 300 ppi) of pieces of art that consist, at their core, of one or more microorganisms growing on agar. (asm.org)
Gelatin1
- Agar gives smoothness to sherbets and ices in combination with other gums like locust bean gum at such combination of 0.12% agar, 0.07% LBG and 0.2% gelatin. (fao.org)
Assay2
Jelly1
- The yellow is a jelly-like growth medium called agar that feeds the bacteria, and the orange along the borders was added to enhance the sunburst effect. (nih.gov)
Nutrient1
- Nutrient Agar is ideal for demonstration and teaching purposes where a more prolonged survival of cultures at ambient temperature is often required without risk of overgrowth that can occur with more nutritious substrate. (thomassci.com)
Ingredients1
- once all the other ingredients are in solution, add the agar. (nih.gov)
Powder1
- The Aspergillus screening plates contain four quadrants with RPMI 1640 2% glucose agar: one growth control well with no antifungal powder, one voriconazole well, one itraconazole well, and one posaconazole well. (cdc.gov)
Colonies2
- Production of a hyphal fringe around colonies grown on Pal's agar (sunflower seed agar) at 30 degrees C for 48 to 72 h provides a simple means of discriminating between isolates of C. dubliniensis and C. albicans with 100% accuracy. (nih.gov)
- In this short video, the agar (yellow) is seeded with bacterial colonies and, through the magic of time-lapse photography, you can see the growth of the colonies into what appears to be a lovely bouquet of delicate flowers. (nih.gov)
Search1
- Agar Corporation is always in search of dynamic and energetic candidates to join its team. (agarcorp.com)
Differentiation1
- A chromogenic Agar for enumeration detection and differentiation of Clostridium sp . (sigmaaldrich.com)
Plates1
- 3) Whether inoculated with bacteria or not, store the plates upside down to avoid condensation buildup on the surface of the agar. (nih.gov)
Meat1
- To maintain the freshness of meat products , an agar - sodium alginate (AS) bilayer antibacterial film incorporated with ginger essential oil (GEO) was developed. (bvsalud.org)
Bacteriological1
- Bacteriological agar is commonly used as a culture medium for microorganism. (sigmaaldrich.com)
Media2
- Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. (wikipedia.org)
- [media] Agar Steps is located on the western side of Observatory Hill and connect Kent Street and Upper Fort Street. (dictionaryofsydney.org)
Bacterial1
- In the 1920s, Sir Alexander Fleming, the Scottish biologist who discovered penicillin, was so taken by this phenomenon that he developed his own palette of bacterial "paints" that he used in his spare time to create colorful pictures of houses, ballerinas, and other figures on the agar [1]. (nih.gov)
Diffusion3
- Zone of inhibition (50 mm) for penicillin G disk diffusion method on Leptospira Vanaporn Wuthiekanun (LVW) agar for Leptospira interrogans serovar Autumnalis strain NR-20161. (ajtmh.org)
- The aim of this study was to evaluate the antimicrobial activity of repair materials of endodontic use MTA/Bio Angelus®, white Portland cement, white MTA Angelus® and white MTA ProRoot® on Enterococcus faecalis, Escherichia coli and Staphylococcus aureus by the agar diffusion test and the direct contact test. (bvsalud.org)
- The results showed that MTA/Bio Angelus® presented superior antimicrobial activity against all bacteria in the agar diffusion test and in the direct contact test. (bvsalud.org)
Mixture1
- The chocolate is melted and the agar added, 35 ml of 1.5% agar solution for every 100 g of chocolate or 350 ml of warm agar solution to 1 kg of molten chocolate, stirred continuously until the mixture is well mixed and homogenized. (fao.org)
Application2
- The application of agar as a food additive in Japan is alleged to have been discovered in 1658 by Mino Tarōzaemon (美濃 太郎左衞門), an innkeeper in current Fushimi-ku, Kyoto who, according to legend, was said to have discarded surplus seaweed soup (Tokoroten) and noticed that it gelled later after a winter night's freezing. (wikipedia.org)
- Effects of ginger essential oil on physicochemical and structural properties of agar-sodium alginate bilayer film and its application to beef refrigeration. (bvsalud.org)
Methods1
- What are the best methods for entering Vietnam Agar Agar Gum Market? (menafn.com)
Actions1
- Dr. Agar and MEI are not responsible for any actions you take as a result of watching these talks. (homedialysis.org)
Resistance2
Growth2
- Nutrients must be added to agar for microbial growth. (flinnsci.com)
- What factors are influencing the growth of the Agar Agar Gum Market over the forecast period? (menafn.com)
Heat3
- Heat with agitation to dissolve agar. (fda.gov)
- Make a 1.5% solution of agar by heating water to a boil then adding powdered agar and heat until the agar dissolves. (fao.org)
- Remove the agar solution from the heat while still warm and liquid and add 2.5 to 5 liters of agar sol to 50 kg sausage mass gradually kneading the mass while the agar sol is being added. (fao.org)
Sugar3
University1
- Nicholas Agar is Professor of Philosophy at the University of Waikato, New Zealand, and the author of How to Be Human in the Digital Economy (MIT Press, 2019). (project-syndicate.org)
Water4
- Agar is used in the formulation of water-based, quick-set and slow-set bakery icings needed for wrapped and unwrapped baked goods. (fao.org)
- Put the autoclaved agar solution in the 50⁰C hot water bath and let cool down to 50⁰C. (cdc.gov)
- If the agar starts to cool, place back in the 50⁰C water bath. (cdc.gov)
- This agar is soluble in boiling water but not in cold water. (sigmaaldrich.com)
Page1
- Visit our forums page, Ask Our Experts-John Agar for more Q & A with John! (homedialysis.org)
Medium1
- The medium is more reliable than m-CP Agar and easier to handle. (sigmaaldrich.com)
History3
- Agar fell an agonising two runs short of becoming the first number 11 in cricket history to make a hundred, as he and Hughes (81no) carried Australia to 280 all out on the second afternoon. (irishtimes.com)
- Hughes and Agar were in no mood to go quietly, though, and not before they had chalked up several notable pieces of cricket history and altered the complexion of this eventful match, anyway. (irishtimes.com)
- But, Dr. Agar does not know YOU or your health history. (homedialysis.org)
Industries1
- However, with the outbreak of World War II, many nations were forced to establish domestic agar industries in order to continue microbiological research. (wikipedia.org)
Back1
- Shellshocked England appeared to run out of ideas, holding back Broad - passed fit to bowl at start of play, despite the shoulder injury he suffered last night - for almost two-and-a-half hours as Agar and Hughes extended the morning session 30 minutes past the scheduled lunch break. (irishtimes.com)
Time1
- A cake icing with a slightly slower setting time may be prepared by first dry-blending agar with salt, emulsifier, dextrose, starch and sucrose. (fao.org)
Food2
- The great bulk of agar produced worldwide is used in the food industry. (fao.org)
- Agar-agar serves as a preservative in food processing. (sigmaaldrich.com)
Weight1
- For sausage processing agar keeps the moisture and prevents evaporation and drying up of the sausage and prevents weight loss and preserves the sausage, too. (fao.org)
Current1
- Fleming's enthusiasm for agar art lives on among the current generation of microbiologists. (nih.gov)
Version2
- The first version of Agar Steps is shown on drawings signed by the City Engineer, Edward Bell , in 1869. (dictionaryofsydney.org)
- See Agar m for the male version. (nordicnames.de)
Collection1
- On track to becoming the UK's largest Passivhaus scheme, this substantial regeneration masterplan has transformed the southern portion of Camden's Agar Grove Estate, bringing with it a collection of different residential building typologies with a strong urban identity, and so creating a new and appropriate streetscape. (architecture.com)